Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005386
Khedidja Rennak, Mira Chikhaoui, Fatima Mahouz
Background: Improved reproduction requires understanding the biometry of goats’ female reproductive system. Objectives: The present study was conducted to record the biometry of the female genital organs of the Arabia (Arbia) goats in Algeria according to age, body condition score, and pregnancy. Methods: A total of 149 reproductive tracts (from 80.54% non-pregnant and 19.46% pregnant goats) from three slaughterhouses were collected and measured. Each organ’s length, width, thickness, and weight were measured using slide calipers and an electric weighing balance. The ages of the fetuses in pregnant goats were determined by measuring their crown-rump length. Analysis was carried out using SPSS software, version 21. Results: The reproductive tract dimensions increase with age, body condition score, and stages of pregnancy. The left ovary’s weight, length, and width showed very significant (P<0.001) increases across age. The measurements of the uterine horn (except thickness) increased significantly (P<0.001) with age. The fallopian tube, the uterine body, and the cervix measures showed significant increases (P<0.001) between the first and third age groups. The ovary and the uterine body dimensions showed highly significant (P<0.001) increases across body condition scores. Pregnancy had no significant effect on the ovary and cervix dimensions. The uterine horn measurements showed significant increases (P<0.05) throughout gestation, and those of the uterine body showed highly significant increases (P<0.001) along the pregnancy stages. Conclusion: This study will provide baseline information on the morphology of the female Arbia breed reproductive system.
{"title":"iometrical Changes in Reproductive Tract of Arbia Goats According to Age, Body Condition, and Pregnancy","authors":"Khedidja Rennak, Mira Chikhaoui, Fatima Mahouz","doi":"10.32598/ijvm.18.3.1005386","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005386","url":null,"abstract":"Background: Improved reproduction requires understanding the biometry of goats’ female reproductive system. Objectives: The present study was conducted to record the biometry of the female genital organs of the Arabia (Arbia) goats in Algeria according to age, body condition score, and pregnancy. Methods: A total of 149 reproductive tracts (from 80.54% non-pregnant and 19.46% pregnant goats) from three slaughterhouses were collected and measured. Each organ’s length, width, thickness, and weight were measured using slide calipers and an electric weighing balance. The ages of the fetuses in pregnant goats were determined by measuring their crown-rump length. Analysis was carried out using SPSS software, version 21. Results: The reproductive tract dimensions increase with age, body condition score, and stages of pregnancy. The left ovary’s weight, length, and width showed very significant (P<0.001) increases across age. The measurements of the uterine horn (except thickness) increased significantly (P<0.001) with age. The fallopian tube, the uterine body, and the cervix measures showed significant increases (P<0.001) between the first and third age groups. The ovary and the uterine body dimensions showed highly significant (P<0.001) increases across body condition scores. Pregnancy had no significant effect on the ovary and cervix dimensions. The uterine horn measurements showed significant increases (P<0.05) throughout gestation, and those of the uterine body showed highly significant increases (P<0.001) along the pregnancy stages. Conclusion: This study will provide baseline information on the morphology of the female Arbia breed reproductive system.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"347 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141691505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005502
B. Albadrani, M. Al-Farwachi, Muhammad Naeem Iqbal, A. Ashraf
Malaria, a mosquito-borne infectious disease caused by the parasite Plasmodium, has been predominantly associated with humans. However, recent studies have uncovered a potential reservoir for Plasmodium spp., within livestock populations. Malaria, caused by the parasitic protozoan Plasmodium spp., is a deadly disease that affects millions of people worldwide. Although human is the primary host for the malaria parasite, it has been discovered that certain animals can also serve as reservoirs. One such potential reservoir is livestock populations, as they have been found to carry Plasmodium spp. parasites. This article will discuss the implications of livestock being potential reservoirs for malaria and the importance of understanding this relationship for effective disease control. Livestock populations have long been overlooked as potential reservoirs for malaria transmission, but recent advancements in diagnostic techniques and surveillance studies have sparked interest in exploring this field. By prioritizing thorough assessments of zoonotic transmission risks, we can gain valuable insights into the role of livestock in malaria transmission and develop effective strategies to reduce the burden of this devastating disease.
{"title":"The Implications of Malaria in Livestock: Reservoirs, Challenges, and Future Directions","authors":"B. Albadrani, M. Al-Farwachi, Muhammad Naeem Iqbal, A. Ashraf","doi":"10.32598/ijvm.18.3.1005502","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005502","url":null,"abstract":"Malaria, a mosquito-borne infectious disease caused by the parasite Plasmodium, has been predominantly associated with humans. However, recent studies have uncovered a potential reservoir for Plasmodium spp., within livestock populations. Malaria, caused by the parasitic protozoan Plasmodium spp., is a deadly disease that affects millions of people worldwide. Although human is the primary host for the malaria parasite, it has been discovered that certain animals can also serve as reservoirs. One such potential reservoir is livestock populations, as they have been found to carry Plasmodium spp. parasites. This article will discuss the implications of livestock being potential reservoirs for malaria and the importance of understanding this relationship for effective disease control. Livestock populations have long been overlooked as potential reservoirs for malaria transmission, but recent advancements in diagnostic techniques and surveillance studies have sparked interest in exploring this field. By prioritizing thorough assessments of zoonotic transmission risks, we can gain valuable insights into the role of livestock in malaria transmission and develop effective strategies to reduce the burden of this devastating disease.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"58 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141691061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005521
Hatice Yaren Kuloğlu, Murat Boydak
Background: The present study aimed at determining histological structure and histochemical properties of the henna partridge (Alectoris chukar) Harderian gland periodically. Objectives: For this purpose, the Harderian glands of 12 (6 females + 6 males) healthy henna partridges aged 3 and 6 months were used. Methods: Totally removed Harderian gland tissue samples were kept in 10% neutral formolin for 36 hours and fixed. After washing, dehydrating, and polishing with known histological techniques, tissue pieces were blocked in paraffin. Results: We found that a thin connective tissue capsule surrounds the Harderian gland of the henna partridge and that the capsule sends septums into the gland and divides the gland into lobes and lobules. It was observed that the corpus glandulae consist of low or high prismatic epithelial cells. Within each lobe, there is a single main draining channel which has a very large lumen along with primary draining channels. Primary and main draining channel epithelial cells have a single-layered cuboidal structure. By Gordon-Sweet staining, it was found that reticular fiber bundles started from the connective tissue capsule that surrounded the organ and spread by branching. The reticular fiber bundles extended in thin strands to the periphery of the corpus glandulae. These reticular fiber bundles in the regions where the connective tissue septums widened are thicker and form a network. The reticular fiber bundles are seen in the basal part of the epithelial cells forming the main draining channel and especially in the crypt areas. Reticular fibers are also found around blood vessels. Strong Alcian blue (AB)-positive epithelial cells are observed in the corpus glandulae in AB pH=2.5 staining, but no periodic acid-Schiff (PAS)-positive epithelial cells are observed in PAS staining. While the majority of primary draining channel cells show a strong AB-positive reaction, a few cells showed a weak PAS-positive reaction. Regarding the main draining channel epithelium, goblet cells show a weak and strong AB-positive reaction in AB pH=2.5 staining method, respectively and a weak PAS-positive reaction in PAS staining method. Conclusion: In line with these findings, no significant difference was found in terms of general histological structure and histochemical properties of the 3- and 6-month-old henna partridge Harderian gland.
背景：本研究旨在定期确定鸡冠花（Alectoris chukar）哈氏腺的组织学结构和组织化学特性。研究目的：为此，研究人员使用了 12 只（6 只雌性 + 6 只雄性）3 个月和 6 个月的健康鸡冠花鹧鸪的硬腺。方法将完全摘除的硬腺组织样本在 10%的中性福尔马林中保存 36 小时并固定。用已知的组织学技术清洗、脱水和抛光后，用石蜡将组织块封存。结果：我们发现，鸡冠花鹧鸪的哈氏腺周围有一层薄的结缔组织囊，囊将隔膜送入腺体，并将腺体分成小叶和小叶。据观察，腺体由低或高的棱形上皮细胞组成。每个腺叶内都有一个主引流道，该引流道的管腔非常大，同时还有主引流道。初级引流道和主引流道上皮细胞呈单层立方体结构。通过戈登-斯weet 染色法发现，网状纤维束从包围器官的结缔组织囊开始，以分支的方式扩散。网状纤维束细细地延伸到腺体外围。在结缔组织隔增宽的区域，网状纤维束较粗并形成网络。网状纤维束出现在上皮细胞的基底部分，形成主要的排水通道，尤其是在隐窝区域。网状纤维也出现在血管周围。在 pH=2.5 的 AB 染色中，可在腺体中观察到强阿尔新蓝（AB）阳性上皮细胞，但在 PAS 染色中未观察到周期性酸-希夫（PAS）阳性上皮细胞。虽然大多数主引流通道细胞显示出强烈的 AB 阳性反应，但也有少数细胞显示出微弱的 PAS 阳性反应。在主引流道上皮细胞中，鹅口疮细胞在 AB pH=2.5 染色法中分别显示弱和强 AB 阳性反应，在 PAS 染色法中显示弱 PAS 阳性反应。结论：与上述研究结果一致，3 个月和 6 个月雏鸡哈氏腺的总体组织学结构和组织化学性质均无明显差异。
{"title":"A Periodic Comparison of Harderian Gland in Henna Partridge (Alectoris chukar) According to Different Developmental Stages","authors":"Hatice Yaren Kuloğlu, Murat Boydak","doi":"10.32598/ijvm.18.3.1005521","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005521","url":null,"abstract":"Background: The present study aimed at determining histological structure and histochemical properties of the henna partridge (Alectoris chukar) Harderian gland periodically. Objectives: For this purpose, the Harderian glands of 12 (6 females + 6 males) healthy henna partridges aged 3 and 6 months were used. Methods: Totally removed Harderian gland tissue samples were kept in 10% neutral formolin for 36 hours and fixed. After washing, dehydrating, and polishing with known histological techniques, tissue pieces were blocked in paraffin. Results: We found that a thin connective tissue capsule surrounds the Harderian gland of the henna partridge and that the capsule sends septums into the gland and divides the gland into lobes and lobules. It was observed that the corpus glandulae consist of low or high prismatic epithelial cells. Within each lobe, there is a single main draining channel which has a very large lumen along with primary draining channels. Primary and main draining channel epithelial cells have a single-layered cuboidal structure. By Gordon-Sweet staining, it was found that reticular fiber bundles started from the connective tissue capsule that surrounded the organ and spread by branching. The reticular fiber bundles extended in thin strands to the periphery of the corpus glandulae. These reticular fiber bundles in the regions where the connective tissue septums widened are thicker and form a network. The reticular fiber bundles are seen in the basal part of the epithelial cells forming the main draining channel and especially in the crypt areas. Reticular fibers are also found around blood vessels. Strong Alcian blue (AB)-positive epithelial cells are observed in the corpus glandulae in AB pH=2.5 staining, but no periodic acid-Schiff (PAS)-positive epithelial cells are observed in PAS staining. While the majority of primary draining channel cells show a strong AB-positive reaction, a few cells showed a weak PAS-positive reaction. Regarding the main draining channel epithelium, goblet cells show a weak and strong AB-positive reaction in AB pH=2.5 staining method, respectively and a weak PAS-positive reaction in PAS staining method. Conclusion: In line with these findings, no significant difference was found in terms of general histological structure and histochemical properties of the 3- and 6-month-old henna partridge Harderian gland.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"169 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141712362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005369
Ibsa Tasse
Background: Bovine paramphistomosis is one of cattle’s most important parasitic diseases, causing mortality and economic losses in various parts of Ethiopia. Objectives: A cross-sectional study was carried out in the Fedis District from April 2019 to October 2020 to determine the prevalence of Paramphistomum and its associated factors in cattle killed at Boko slaughterhouse. Methods: A total of 384 slaughtered cattle were selected by systematic random sampling. Then, a post-mortem examination of the rumen and reticulum was conducted to check the presence or absence of adult Paramphistomum. The parasite was examined macroscopically and microscopically to study the morphology of adult flukes. Results: Of 384 examined cattle, Paramphistomum was found in 156(40.6%). Of the 156 positive samples, 52.34%, 33.06%, and 14.6% were infected with Paramphistomum clavula, Paramphistomum cervi, and mixed infection, respectively. In this study, the highest infection rate was recorded in the wet season. Among assessed factors, body condition and origin of animals had significant correlations (P<0.05) with infection. Conclusion: This finding indicated the highest infection of Paramphistomum was recorded in cattle. Therefore, the farmers should practice mass deworming of animals using selected anthelmintic therapy for flukes.
{"title":"Prevalence of Paramphistomum and Its Associated Factors in Cattle Slaughtered at Boko Slaughterhouse, Fedis District, Eastern Hararghe Zone, Ethiopia","authors":"Ibsa Tasse","doi":"10.32598/ijvm.18.3.1005369","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005369","url":null,"abstract":"Background: Bovine paramphistomosis is one of cattle’s most important parasitic diseases, causing mortality and economic losses in various parts of Ethiopia. Objectives: A cross-sectional study was carried out in the Fedis District from April 2019 to October 2020 to determine the prevalence of Paramphistomum and its associated factors in cattle killed at Boko slaughterhouse. Methods: A total of 384 slaughtered cattle were selected by systematic random sampling. Then, a post-mortem examination of the rumen and reticulum was conducted to check the presence or absence of adult Paramphistomum. The parasite was examined macroscopically and microscopically to study the morphology of adult flukes. Results: Of 384 examined cattle, Paramphistomum was found in 156(40.6%). Of the 156 positive samples, 52.34%, 33.06%, and 14.6% were infected with Paramphistomum clavula, Paramphistomum cervi, and mixed infection, respectively. In this study, the highest infection rate was recorded in the wet season. Among assessed factors, body condition and origin of animals had significant correlations (P<0.05) with infection. Conclusion: This finding indicated the highest infection of Paramphistomum was recorded in cattle. Therefore, the farmers should practice mass deworming of animals using selected anthelmintic therapy for flukes.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005384
Ali Salavati, S. M. Peighambari, A. Yazdani, J. Razmyar
Background: Histomoniasis is caused by the protozoan Histomonas meleagridis with an intermediate host of Heterakis gallinarum, which results in ulceration of the ceca walls, enlargement of the ceca by large casts, mesenteric inflammation, and liver necrosis. This disease is very important in Iran’s growing turkey breeding industry. Objectives: The present study aims to evaluate the relative frequency of H. meleagridis infection in different turkey flocks to draw a cross-sectional picture of H. meleagridis infection in Golestan, Mazandaran, Guilan, and Tehran provinces of Iran. Methods: This study is a cross-sectional survey of H. meleagridis infection during spring. Dropping samples were taken from backyard and commercial turkey flocks. After taking the fecal samples, they were investigated by Giemsa staining under a light microscope. A PCR test was performed to confirm the diagnosis of infection. Results: Out of 240 samples (from 19 flocks), 20 infected samples were detected by direct microscopic observation of H. meleagridis, and PCR confirmed 15 samples. Conclusion: The results of this study showed that the relative frequency of H. meleagridis infection was lower than in similar studies in other parts of the world. This finding may be due to Iran’s less widespread use of turkey production. Considering the growth of the turkey production industry in Iran over the last decade and its further production over the next few years, it is necessary to evaluate histomoniasis.
背景：组织单胞菌病是由原生动物组织单胞菌（Histomonas meleagridis）引起的，中间宿主是Heterakis gallinarum，该病会导致盲肠壁溃疡、盲肠肿大、肠系膜发炎和肝脏坏死。这种疾病对伊朗日益增长的火鸡养殖业非常重要。研究目的本研究旨在评估不同火鸡群中 H. meleagridis 感染的相对频率，以绘制伊朗戈勒斯坦省、马赞达兰省、吉兰省和德黑兰省 H. meleagridis 感染的横断面图。研究方法本研究是对春季 H. meleagridis 感染情况的横断面调查。从散养火鸡群和商业火鸡群中采集滴落样本。采集粪便样本后，用 Giemsa 染色法在光学显微镜下进行检测。进行 PCR 检测以确诊感染。结果：在 240 份样本（来自 19 个鸡群）中，有 20 份样本通过直接显微镜观察发现了 H. meleagridis，有 15 份样本通过 PCR 检测确诊。结论研究结果表明，H. meleagridis 感染的相对频率低于世界其他地区的类似研究。造成这一结果的原因可能是伊朗火鸡生产不太普遍。考虑到伊朗火鸡生产业在过去十年的增长以及未来几年的进一步生产，有必要对组织单胞菌病进行评估。
{"title":"The Relative Frequency of Histomonas meleagridis Infection in Turkey Flocks in Some Provinces of Iran","authors":"Ali Salavati, S. M. Peighambari, A. Yazdani, J. Razmyar","doi":"10.32598/ijvm.18.3.1005384","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005384","url":null,"abstract":"Background: Histomoniasis is caused by the protozoan Histomonas meleagridis with an intermediate host of Heterakis gallinarum, which results in ulceration of the ceca walls, enlargement of the ceca by large casts, mesenteric inflammation, and liver necrosis. This disease is very important in Iran’s growing turkey breeding industry. Objectives: The present study aims to evaluate the relative frequency of H. meleagridis infection in different turkey flocks to draw a cross-sectional picture of H. meleagridis infection in Golestan, Mazandaran, Guilan, and Tehran provinces of Iran. Methods: This study is a cross-sectional survey of H. meleagridis infection during spring. Dropping samples were taken from backyard and commercial turkey flocks. After taking the fecal samples, they were investigated by Giemsa staining under a light microscope. A PCR test was performed to confirm the diagnosis of infection. Results: Out of 240 samples (from 19 flocks), 20 infected samples were detected by direct microscopic observation of H. meleagridis, and PCR confirmed 15 samples. Conclusion: The results of this study showed that the relative frequency of H. meleagridis infection was lower than in similar studies in other parts of the world. This finding may be due to Iran’s less widespread use of turkey production. Considering the growth of the turkey production industry in Iran over the last decade and its further production over the next few years, it is necessary to evaluate histomoniasis.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"70 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141696125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-01DOI: 10.32598/ijvm.18.3.1005389
Olushola Emmanuel Adeleye, Temtope Ajala, Oluwatodimu Adewole Adekoya, A. Adeleye
Background: Diabetes mellitus (DM) is a metabolic disorder characterized by an elevated blood sugar level due to problems with insulin synthesis, effect, or both. Various clinical signs follow DM: Hyperglycemia, polydipsia, polyuria, and polyphagia. Worldwide prevalence is high and predicted to be 592 million by 2035. Animal models are used in the study of diabetes due to ethical issues. Although the streptozotocin (STZ) model is frequently used, it is unreliable due to unexplained acute toxicity and effective dose variability. Objectives: This research was conducted to determine the effective dose regimen of STZ for inducing diabetes in Wister rats. Methods: A total of 28 male Wistar rats (160-190 g) were randomly divided into 4 groups (each 7 rats) and monitored for 21 days after diabetes induction with STZ: Control (CTR), diabetics (DIA)1 (60 mg/kg STZ), DIA2 (60 mg/kg STZ twice at 0 and 24 hours), and DIA3 (60 mg/kg STZ thrice at 0, 24 and 48 hours). Plasma glucose was determined with a glucometer. Body weights, feed intake, and fecal output were weighed with a digital balance, while water intake and urine output were measured with a measuring cylinder. Analyses of data obtained were performed using a one-way ANOVA and Tukey’s test at a significance level of P≤0.05. Results: There was a significant (P<0.05) decrease in body weight of the diabetic groups (-15.53%±1.2%, -26.8%±1.2%, -28.5%±1.9%) compared to the CTR (10.5%±2.5%). There was a significant (P<0.05) increase in fasting blood glucose concentrations (135.2±9.0, 273.2±6.5, 257.0±5.3 mg/dL) in the people with diabetes compared to the CTR (79.3±1.1 mg/dL). Water intake (56.9±0.9, 72.1±1.7, 77.8±5.5 mL), feed intake (19.4±0.6, 23.3±1.9, 42.1±2.1 g), voided urine (6.34±0.1, 8.39±0.88, 9.8±0.50 mL) and voided feces (10.4±0.26, 11.7±0.43, 8.5±0.17 g) in the diabetic groups increased significantly (P<0.05) compared to the CTR (26.5±0.8 mL, 13.4±0.3 g, 1.84±0.08 mL, and 6.5±0.33 g, respectively). Conclusion: The dose regimen of 60 mg/kg STZ administered intraperitoneally twice (24 hours apart) sustained diabetes for 21 days. We recommend adopting this dose regimen in STZ-induced diabetic studies in male Wistar rats.
{"title":"Effective Dose Regimen of Streptozotocin for Inducing Diabetes in a Rat Model","authors":"Olushola Emmanuel Adeleye, Temtope Ajala, Oluwatodimu Adewole Adekoya, A. Adeleye","doi":"10.32598/ijvm.18.3.1005389","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005389","url":null,"abstract":"Background: Diabetes mellitus (DM) is a metabolic disorder characterized by an elevated blood sugar level due to problems with insulin synthesis, effect, or both. Various clinical signs follow DM: Hyperglycemia, polydipsia, polyuria, and polyphagia. Worldwide prevalence is high and predicted to be 592 million by 2035. Animal models are used in the study of diabetes due to ethical issues. Although the streptozotocin (STZ) model is frequently used, it is unreliable due to unexplained acute toxicity and effective dose variability. Objectives: This research was conducted to determine the effective dose regimen of STZ for inducing diabetes in Wister rats. Methods: A total of 28 male Wistar rats (160-190 g) were randomly divided into 4 groups (each 7 rats) and monitored for 21 days after diabetes induction with STZ: Control (CTR), diabetics (DIA)1 (60 mg/kg STZ), DIA2 (60 mg/kg STZ twice at 0 and 24 hours), and DIA3 (60 mg/kg STZ thrice at 0, 24 and 48 hours). Plasma glucose was determined with a glucometer. Body weights, feed intake, and fecal output were weighed with a digital balance, while water intake and urine output were measured with a measuring cylinder. Analyses of data obtained were performed using a one-way ANOVA and Tukey’s test at a significance level of P≤0.05. Results: There was a significant (P<0.05) decrease in body weight of the diabetic groups (-15.53%±1.2%, -26.8%±1.2%, -28.5%±1.9%) compared to the CTR (10.5%±2.5%). There was a significant (P<0.05) increase in fasting blood glucose concentrations (135.2±9.0, 273.2±6.5, 257.0±5.3 mg/dL) in the people with diabetes compared to the CTR (79.3±1.1 mg/dL). Water intake (56.9±0.9, 72.1±1.7, 77.8±5.5 mL), feed intake (19.4±0.6, 23.3±1.9, 42.1±2.1 g), voided urine (6.34±0.1, 8.39±0.88, 9.8±0.50 mL) and voided feces (10.4±0.26, 11.7±0.43, 8.5±0.17 g) in the diabetic groups increased significantly (P<0.05) compared to the CTR (26.5±0.8 mL, 13.4±0.3 g, 1.84±0.08 mL, and 6.5±0.33 g, respectively). Conclusion: The dose regimen of 60 mg/kg STZ administered intraperitoneally twice (24 hours apart) sustained diabetes for 21 days. We recommend adopting this dose regimen in STZ-induced diabetic studies in male Wistar rats.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"82 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Prolonged stress plays an essential role in depression disorder through brain inflammation and neurotransmitter imbalances. The natural plant antioxidant is promising to resist the negative impact of stress. Objectives: This study aimed to analyze the effect of total anthocyanin (ANC) extracts from purple sweet potatoes (PSP) on brain neurotransmitters, inflammation, and locomotor behavior in the chronic-stressed mice model. Methods: Twenty male adult BALB/c mice were assigned to control, stress (STR), STR+ANC (10 mg/kg body weight [BW]), STR+ANC (20 mg/kg BW), and STR+ANC (40 mg/kg BW). Restraint stress was applied 2 h/d for 14 days. The enzyme-linked immunosorbent assay (ELISA) was performed to measure brain dopamine, gamma-aminobutyric acid (GABA), and corticosterone levels. The locomotor behavior was analyzed using an open field test before and after ANC treatment. In silico, molecular docking was carried out between ANC and monoamine oxidase-B (MAO-B) enzyme. Results: Administration of ANC decreased brain corticosterone levels. The dopamine neurotransmitter decreased in the stress-induced group and increased following ANC treatment. Increased GABA levels were observed in the stressed and treated groups. Locomotor analysis showed reduced total distance movement and velocity after ANC treatment. Molecular prediction showed that ANC can inhibit the MAO-B enzymes. Conclusion: The ANC extracted from PSP relieved brain inflammation and modified the neurotransmitters of dopamine and GABA, affecting the locomotor function of chronically stressed-induced mice. Furthermore, in vivo studies are necessary to evaluate the molecular mechanism of ANC from PSP in chronic stress exposure, particularly on MAO enzyme regulation.
{"title":"Anthocyanin Extract From Purple Sweet Potato Improving Neurotransmitter and Locomotor in Chronic Stressed-mice","authors":"Nia Kurnianingsih, Ariella Ramadhini Hakim, Daffa Salsabila, Agwin Fahmi Fahanani, Novita Titis Harbiyanti, Retty Ratnawati","doi":"10.32598/ijvm.18.3.1005405","DOIUrl":"https://doi.org/10.32598/ijvm.18.3.1005405","url":null,"abstract":"Background: Prolonged stress plays an essential role in depression disorder through brain inflammation and neurotransmitter imbalances. The natural plant antioxidant is promising to resist the negative impact of stress. Objectives: This study aimed to analyze the effect of total anthocyanin (ANC) extracts from purple sweet potatoes (PSP) on brain neurotransmitters, inflammation, and locomotor behavior in the chronic-stressed mice model. Methods: Twenty male adult BALB/c mice were assigned to control, stress (STR), STR+ANC (10 mg/kg body weight [BW]), STR+ANC (20 mg/kg BW), and STR+ANC (40 mg/kg BW). Restraint stress was applied 2 h/d for 14 days. The enzyme-linked immunosorbent assay (ELISA) was performed to measure brain dopamine, gamma-aminobutyric acid (GABA), and corticosterone levels. The locomotor behavior was analyzed using an open field test before and after ANC treatment. In silico, molecular docking was carried out between ANC and monoamine oxidase-B (MAO-B) enzyme. Results: Administration of ANC decreased brain corticosterone levels. The dopamine neurotransmitter decreased in the stress-induced group and increased following ANC treatment. Increased GABA levels were observed in the stressed and treated groups. Locomotor analysis showed reduced total distance movement and velocity after ANC treatment. Molecular prediction showed that ANC can inhibit the MAO-B enzymes. Conclusion: The ANC extracted from PSP relieved brain inflammation and modified the neurotransmitters of dopamine and GABA, affecting the locomotor function of chronically stressed-induced mice. Furthermore, in vivo studies are necessary to evaluate the molecular mechanism of ANC from PSP in chronic stress exposure, particularly on MAO enzyme regulation.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"239 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141692582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.32598/ijvm.18.2.1005347
Razieh Heidari, V. Noaman, Hedieh Jafari
Background: Anaplasma sp. is a blood protozoon that causes economic damage to the livestock industry. Therefore, studying this disease’s epidemiology and distribution pattern in different regions is essential. Objectives: This study aimed to investigate the variety of infections of the Anaplasma sp. in the sheep population of Khuzestan Province in Iran. Methods: A total of 200 sheep blood samples were randomly collected and examined using specific nested polymerase chain reaction (nPCR) based on the 16S rRNA gene. Results: The prevalence of Anaplasma phagocytophilum was 17%, and infected sheep had no clinical signs. The effective risk factors in the spread of infection in Khuzestan Province include sheep aged 3-5 years, low sanitation, high-density farms, use of acaricides in the field, and hot season (P≤0.05). There was no significant association between the occurrence of A. phagocytophilum infection and variables of altitude, farm type, vectors, distance from other farms, and sex. Conclusion: Since the infection often has no clinical symptoms, identifying the risk factors and epidemiology is essential to develop control and prevention planning.
{"title":"Prevalence, Risk Factors, and Molecular Epidemiology of Anaplasma phagocytophilum in Sheep Raising in Khuzestan Province, Iran","authors":"Razieh Heidari, V. Noaman, Hedieh Jafari","doi":"10.32598/ijvm.18.2.1005347","DOIUrl":"https://doi.org/10.32598/ijvm.18.2.1005347","url":null,"abstract":"Background: Anaplasma sp. is a blood protozoon that causes economic damage to the livestock industry. Therefore, studying this disease’s epidemiology and distribution pattern in different regions is essential. Objectives: This study aimed to investigate the variety of infections of the Anaplasma sp. in the sheep population of Khuzestan Province in Iran. Methods: A total of 200 sheep blood samples were randomly collected and examined using specific nested polymerase chain reaction (nPCR) based on the 16S rRNA gene. Results: The prevalence of Anaplasma phagocytophilum was 17%, and infected sheep had no clinical signs. The effective risk factors in the spread of infection in Khuzestan Province include sheep aged 3-5 years, low sanitation, high-density farms, use of acaricides in the field, and hot season (P≤0.05). There was no significant association between the occurrence of A. phagocytophilum infection and variables of altitude, farm type, vectors, distance from other farms, and sex. Conclusion: Since the infection often has no clinical symptoms, identifying the risk factors and epidemiology is essential to develop control and prevention planning.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"24 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140772423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.32598/ijvm.18.2.1005367
Layan Yaseen Khalil, Nadia Hamid Mohammed, N. Alhayali
Background: Farmers raise rabbits in abundance in Iraq. Scabies infestation is one of the most prevalent diseases in rabbits. The current study investigates the rabbits’ infestation with scabies that cause severe complications such as itchy alopecia, hyperkeratosis, anorexia, self-trauma, and weight loss. In short, the disease has high morbidity and mortality and creates heavy economic losses for farmers. Objectives: This study was designed to detect mange infestation in rabbits using microscopic examination of skin scraping. This study is the first to investigate mange infestation in rabbits in Mosul City, Iraq. Methods: A total of 130 rabbits were examined, and their skin lesions were scraped. Results: Of 130 rabbits, 56(43.1%) were infested with mange, including Sarcoptes scabiei var. cuniculi (n=50, 38.5%), Psoroptes cuniculi (n=32, 25%), Notoedres cati var. cuniculi (n=26, 20%), Demodex cuniculi (n=12, 9%), and Cheyletiella spp. (n=4, 3%), with the high infestation rate for S. scabiei var. cuniculi and the lowest rate for Cheyletiella spp. The significant lesions were hyperkeratosis followed by alopecia, then pruritus on many body areas (head, ears, abdomen, back, legs, tail, and perineal area). The highest infestation rate was found on the abdomen and back, while the lowest was on the tail and perineal area. There are significant differences according to age, while no significant differences between the males and females and among three types of infestation (single, double, and mixed). Conclusion: The rabbits were infested with several species with different percentages. There was no significant difference in infestation rates between males and females, although there was a difference between younger and older animals. Lastly, a significant difference was clear among the three types of infestation, and the double one was the dominant infestation rate with 50%.
背景：伊拉克农民大量饲养兔子。疥癣是兔子最常见的疾病之一。本研究调查了兔子感染疥疮后引起的严重并发症，如瘙痒性脱发、角化过度、厌食、自残和体重减轻。总之，该病的发病率和死亡率都很高，给养殖户造成了严重的经济损失。研究目的本研究旨在利用皮肤刮片的显微镜检查来检测兔子的疥癣病。这是首次对伊拉克摩苏尔市兔子疥癣病进行调查。研究方法共检查了 130 只兔子，并对它们的皮损进行了刮片检查。结果：130 只兔子中130只兔子中有56只（43.1%）患有疥癣，包括疥螨变种（Sarcoptes scabiei var. cuniculi，n=50，38.5%）、疥癬螨（Psoroptes cuniculi，n=32，25%）、疥癬螨变种（Notoedres cati var. cuniculi，n=26，20%）、疥癬毛囊虫（Demodex cuniculi，n=12，9%）和疥癬螨属（Cheyletiella spp.(明显的病变是角化过度，然后是脱发，接着是身体多个部位（头部、耳朵、腹部、背部、腿部、尾部和会阴部）的瘙痒。）腹部和背部的感染率最高，而尾部和会阴部的感染率最低。不同年龄段之间存在明显差异，而雌雄之间以及三种虫害类型（单虫、双虫和混合虫）之间则无明显差异。结论兔子身上有几种不同比例的虫害。雄兔和雌兔的虫害率没有明显差异，但幼兔和老兔的虫害率存在差异。最后，三种侵扰类型之间存在明显差异，其中双侵扰是主要侵扰类型，侵扰率为 50%。
{"title":"Incidence of Mange Infestation in Rabbits","authors":"Layan Yaseen Khalil, Nadia Hamid Mohammed, N. Alhayali","doi":"10.32598/ijvm.18.2.1005367","DOIUrl":"https://doi.org/10.32598/ijvm.18.2.1005367","url":null,"abstract":"Background: Farmers raise rabbits in abundance in Iraq. Scabies infestation is one of the most prevalent diseases in rabbits. The current study investigates the rabbits’ infestation with scabies that cause severe complications such as itchy alopecia, hyperkeratosis, anorexia, self-trauma, and weight loss. In short, the disease has high morbidity and mortality and creates heavy economic losses for farmers. Objectives: This study was designed to detect mange infestation in rabbits using microscopic examination of skin scraping. This study is the first to investigate mange infestation in rabbits in Mosul City, Iraq. Methods: A total of 130 rabbits were examined, and their skin lesions were scraped. Results: Of 130 rabbits, 56(43.1%) were infested with mange, including Sarcoptes scabiei var. cuniculi (n=50, 38.5%), Psoroptes cuniculi (n=32, 25%), Notoedres cati var. cuniculi (n=26, 20%), Demodex cuniculi (n=12, 9%), and Cheyletiella spp. (n=4, 3%), with the high infestation rate for S. scabiei var. cuniculi and the lowest rate for Cheyletiella spp. The significant lesions were hyperkeratosis followed by alopecia, then pruritus on many body areas (head, ears, abdomen, back, legs, tail, and perineal area). The highest infestation rate was found on the abdomen and back, while the lowest was on the tail and perineal area. There are significant differences according to age, while no significant differences between the males and females and among three types of infestation (single, double, and mixed). Conclusion: The rabbits were infested with several species with different percentages. There was no significant difference in infestation rates between males and females, although there was a difference between younger and older animals. Lastly, a significant difference was clear among the three types of infestation, and the double one was the dominant infestation rate with 50%.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"194 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140777788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.32598/ijvm.18.2.1005381
Mohammad Taghi Vajed Ebrahimi, Farhad Mohammadi Gheshlagh, Abbas Parham
Background: Tissue staining is pivotal in histology and histopathology, shouldering a noteworthy role in identifying and classifying tissues and diseases. Due to their non-production of toxic effluents, the utilization of plant-based dyes aligns harmoniously with environmental sustainability and the well-being of laboratory personnel and the general public. Furthermore, this approach is highly cost-effective, further enhancing its appeal. Objectives: This research study explored the feasibility of staining various tissues in mice, such as the liver, kidney, intestine, and cartilage, utilizing a dye extracted from black carrots. Methods: An ethanol extract of 200 g of fresh black carrots (Daucus carota L.) was prepared using 95% ethanol saturated with two different solvents in 200 mL of distilled water. Subsequently, the prepared sections of mice tissue were immersed in the extracted dye solution for 20 minutes, followed by assessment using a light microscope. Hematoxylin-eosin staining was used as a control. Results: The dye extracted from the black carrot using alum and acetic acid successfully stained the cartilage, kidney, intestine, and liver tissues, giving them a bluish-gray coloration. Phytochemical screening further confirmed the presence of anthocyanins in the black carrot extract. Conclusion: The dye derived from black carrots exhibits natural tissue staining capabilities, making it an alternative to hematoxylin-eosin in histology and histopathology laboratories.
{"title":"Using Black Carrot Extracts as an Alternative Biological Dye for Tissue Staining","authors":"Mohammad Taghi Vajed Ebrahimi, Farhad Mohammadi Gheshlagh, Abbas Parham","doi":"10.32598/ijvm.18.2.1005381","DOIUrl":"https://doi.org/10.32598/ijvm.18.2.1005381","url":null,"abstract":"Background: Tissue staining is pivotal in histology and histopathology, shouldering a noteworthy role in identifying and classifying tissues and diseases. Due to their non-production of toxic effluents, the utilization of plant-based dyes aligns harmoniously with environmental sustainability and the well-being of laboratory personnel and the general public. Furthermore, this approach is highly cost-effective, further enhancing its appeal. Objectives: This research study explored the feasibility of staining various tissues in mice, such as the liver, kidney, intestine, and cartilage, utilizing a dye extracted from black carrots. Methods: An ethanol extract of 200 g of fresh black carrots (Daucus carota L.) was prepared using 95% ethanol saturated with two different solvents in 200 mL of distilled water. Subsequently, the prepared sections of mice tissue were immersed in the extracted dye solution for 20 minutes, followed by assessment using a light microscope. Hematoxylin-eosin staining was used as a control. Results: The dye extracted from the black carrot using alum and acetic acid successfully stained the cartilage, kidney, intestine, and liver tissues, giving them a bluish-gray coloration. Phytochemical screening further confirmed the presence of anthocyanins in the black carrot extract. Conclusion: The dye derived from black carrots exhibits natural tissue staining capabilities, making it an alternative to hematoxylin-eosin in histology and histopathology laboratories.","PeriodicalId":14566,"journal":{"name":"Iranian Journal of Veterinary Medicine","volume":"50 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140771315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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