Tatsuhiro Niino, T. Harada, Toshiaki Houjou, S. Yamada, K. Oonishi, Yasuo Sato, H. Saito
{"title":"Metabolomic approach for assessing the mode of action of bioactive compounds on the green algae Pseudokirchneriella subcapitata","authors":"Tatsuhiro Niino, T. Harada, Toshiaki Houjou, S. Yamada, K. Oonishi, Yasuo Sato, H. Saito","doi":"10.11403/JSET.11.83","DOIUrl":"https://doi.org/10.11403/JSET.11.83","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87352500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ascidians are marine invertebrate chordates. We have developed a large-scale oligo DNA microarray of the ascidian Ciona intestinalis. We initially obtained expression patterns of 10,000 genes of this animal during its life cycle. We have now constructed a novel risk assessment system for marine chemical pollutants using ascidian DNA microarrays. First, we identify genes upor down-regulated after exposing ascidians to pollutants. Second, we estimate the effects of the pollutants on the ascidians using array data and information about the life stages highly expressing those genes. Third, to verify the estimation, we investigate the inhibitory effects of pollutants on embryogenesis and metamorphosis in ascidians. From DNA microarray data, we estimated that organotin compounds might affect embryogenesis and metamorphosis in ascidians. We performed bioassay using embryo and larvae and found that a low concentration of the chemicals completely inhibited ascidian embryogenesis and metamorphosis. Our new risk assessment system will be a valuable tool for monitoring marine chemical pollutants.
{"title":"New Risk Assessment of Marine Chemical Pollutants using Ascidians","authors":"K. Azumi","doi":"10.11403/JSET.11.95","DOIUrl":"https://doi.org/10.11403/JSET.11.95","url":null,"abstract":"Ascidians are marine invertebrate chordates. We have developed a large-scale oligo DNA microarray of the ascidian Ciona intestinalis. We initially obtained expression patterns of 10,000 genes of this animal during its life cycle. We have now constructed a novel risk assessment system for marine chemical pollutants using ascidian DNA microarrays. First, we identify genes upor down-regulated after exposing ascidians to pollutants. Second, we estimate the effects of the pollutants on the ascidians using array data and information about the life stages highly expressing those genes. Third, to verify the estimation, we investigate the inhibitory effects of pollutants on embryogenesis and metamorphosis in ascidians. From DNA microarray data, we estimated that organotin compounds might affect embryogenesis and metamorphosis in ascidians. We performed bioassay using embryo and larvae and found that a low concentration of the chemicals completely inhibited ascidian embryogenesis and metamorphosis. Our new risk assessment system will be a valuable tool for monitoring marine chemical pollutants.","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76314027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a prediction method for ecotoxicants by transcript profiling","authors":"H. Yokota","doi":"10.11403/JSET.11.91","DOIUrl":"https://doi.org/10.11403/JSET.11.91","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79793567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Fujita, Y. Morimoto, A. Ogami, Toshihiko Myojyo, I. Tanaka, M. Shimada, Wei-Ning Wang, S. Endoh, K. Uchida, S. Kinugasa, H. Kato, Kayori Akahashi, H. Tao, Masaharu Inada, T. Nakazato, Kazuhiro Yamamoto, H. Fukui, M. Horie, Y. Yoshida, H. Iwahashi, J. Nakanishi
Recently, concern over the influence of manufactured nanoparticles on human health has risen due to advances in the development of nanotechnology. We are interested in the influence of nanoparticles on the pulmonary system at a molecular level. In this study, gene expression profiling of the rat lung after intratracheal instillation or whole-body inhalation exposure to ultrafine nickel oxide(Uf-NiO)particles as a positive control of manufactured nanoparticles such as titanium dioxide, C60 fullerene, carbon nanotube were employed to gain insights into these molecular events. In response to Uf-NiO exposure, there were a large number of highly up-regulated genes associated with the inflammatory response and Mmp12 encoding macrophage metalloelastase 12. These results suggest that Uf-NiO particles lead to acute inflammation immediately after intratracheal instillation or for the inhalation exposure period, and the damaged tissues were repaired in a timedependent manner. Analysis by inductively coupled plasma mass spectrometry and transmission electron microscope revealed that the Uf-NiO particles began to be cleared from the lungs immediately after treatment, and that low levels of the particles were present following exposure. These observations were consistent in gene expression profiling. We conclude that gene expression analysis using DNA microarrays can be extremely useful in assessing the influence of utrafine particles on biological systems.
{"title":"Gene expression profiling to assess the biological effects of manufactured nanomaterials","authors":"K. Fujita, Y. Morimoto, A. Ogami, Toshihiko Myojyo, I. Tanaka, M. Shimada, Wei-Ning Wang, S. Endoh, K. Uchida, S. Kinugasa, H. Kato, Kayori Akahashi, H. Tao, Masaharu Inada, T. Nakazato, Kazuhiro Yamamoto, H. Fukui, M. Horie, Y. Yoshida, H. Iwahashi, J. Nakanishi","doi":"10.11403/JSET.11.69","DOIUrl":"https://doi.org/10.11403/JSET.11.69","url":null,"abstract":"Recently, concern over the influence of manufactured nanoparticles on human health has risen due to advances in the development of nanotechnology. We are interested in the influence of nanoparticles on the pulmonary system at a molecular level. In this study, gene expression profiling of the rat lung after intratracheal instillation or whole-body inhalation exposure to ultrafine nickel oxide(Uf-NiO)particles as a positive control of manufactured nanoparticles such as titanium dioxide, C60 fullerene, carbon nanotube were employed to gain insights into these molecular events. In response to Uf-NiO exposure, there were a large number of highly up-regulated genes associated with the inflammatory response and Mmp12 encoding macrophage metalloelastase 12. These results suggest that Uf-NiO particles lead to acute inflammation immediately after intratracheal instillation or for the inhalation exposure period, and the damaged tissues were repaired in a timedependent manner. Analysis by inductively coupled plasma mass spectrometry and transmission electron microscope revealed that the Uf-NiO particles began to be cleared from the lungs immediately after treatment, and that low levels of the particles were present following exposure. These observations were consistent in gene expression profiling. We conclude that gene expression analysis using DNA microarrays can be extremely useful in assessing the influence of utrafine particles on biological systems.","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81196262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Participation Report on SETAC North America 29th Annual Meeting","authors":"S. Kashiwada","doi":"10.11403/JSET.11.171","DOIUrl":"https://doi.org/10.11403/JSET.11.171","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83842325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Process of JSET-establishment and Inevitability of Filed Investigations for Environmental Assessment of chemical Hazards.","authors":"S. Hatakeyama","doi":"10.11403/JSET.11.51","DOIUrl":"https://doi.org/10.11403/JSET.11.51","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84875018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Udaka, T. Ikemoto, Shin Takahashi, H. Iwata, V. Batoev, E. Petrov, S. Tanabe
{"title":"Accumulation of trace elements in the liver and muscle of Baikal seals (Pusa sibirica)","authors":"M. Udaka, T. Ikemoto, Shin Takahashi, H. Iwata, V. Batoev, E. Petrov, S. Tanabe","doi":"10.11403/JSET.11.117","DOIUrl":"https://doi.org/10.11403/JSET.11.117","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83875091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1.はじめに 構造活性相関(SAR)においては,特異的部 分構造の有無やその数から,カテゴリーを決定 したり,logPow等の2次的パラメータを作成す る必要がある。そのため,定性的あるいは定量 的な活性推定プログラムにおいて最も重要でか つ困難な部分が,問題とする化学物質から各種 部分構造を抽出して数えるプログラムである。 SARプログラム自身が利用でき,部分構造を 記述する方法が公開されていても,その数え方 のプログラムの詳細はほとんど公開されていな い 。数え方は,各開発者のノウハウであり, それを保持することにより開発の優位性を保つ ことができる。PAS(Platform for Assessment from Structure)においては,直線的構造を木 構造のように組み立てることによって化学物質 の構造を指定する。この指定を解釈して問題構 造中のその部分構造を数える方法をF I TS (Fragment Identification by Tree Structure)と 名付け,その方法のノウハウに関して報告する。
1.首先，在结构活性相关(SAR)中，需要根据特异性部分结构的有无及其数量来决定类别，制作logPow等二阶参数。因此，在定性或定量的活性估计程序中，最重要也是最困难的部分就是从问题化学物质中提取各种子结构并计数的程序。SAR程序本身就可以使用，即使公开了描述部分结构的方法，其计数程序的详细情况也几乎没有公开。计数方法是各开发者的专有技术，通过保留该技术可以保持开发优势。PAS(Platform for Assessment from Structure, Platform for Assessment from Structure)是通过像树一样构造直线结构来指定化学物质的结构。解释这个指定，计数问题构造中的那个子结构的方法是Fragment Identification by Tree Structure命名，并就该方法的秘诀进行报告。
{"title":"Know-How in the Development of a Program for QSAR Platform; PAS","authors":"Y. Yoshioka","doi":"10.11403/JSET.11.33","DOIUrl":"https://doi.org/10.11403/JSET.11.33","url":null,"abstract":"1.はじめに 構造活性相関(SAR)においては,特異的部 分構造の有無やその数から,カテゴリーを決定 したり,logPow等の2次的パラメータを作成す る必要がある。そのため,定性的あるいは定量 的な活性推定プログラムにおいて最も重要でか つ困難な部分が,問題とする化学物質から各種 部分構造を抽出して数えるプログラムである。 SARプログラム自身が利用でき,部分構造を 記述する方法が公開されていても,その数え方 のプログラムの詳細はほとんど公開されていな い 。数え方は,各開発者のノウハウであり, それを保持することにより開発の優位性を保つ ことができる。PAS(Platform for Assessment from Structure)においては,直線的構造を木 構造のように組み立てることによって化学物質 の構造を指定する。この指定を解釈して問題構 造中のその部分構造を数える方法をF I TS (Fragment Identification by Tree Structure)と 名付け,その方法のノウハウに関して報告する。","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87914566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Sugimura, T. Kubo, Naoko Murata, K. Urano, T. Shigeoka
{"title":"Development of the efficient umu-test system for evaluation of genotoxicity of water samples","authors":"Y. Sugimura, T. Kubo, Naoko Murata, K. Urano, T. Shigeoka","doi":"10.11403/JSET.11.21","DOIUrl":"https://doi.org/10.11403/JSET.11.21","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75031556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Upma Narang, Ashwani Kumar Thukral, R. Bhardwaj, S. Garg
{"title":"Glutathione-Ascorbate Cycle for Phytoremediation of Mercury by Eichhornia crassipes (Mart.) Solms","authors":"Upma Narang, Ashwani Kumar Thukral, R. Bhardwaj, S. Garg","doi":"10.11403/JSET.11.1","DOIUrl":"https://doi.org/10.11403/JSET.11.1","url":null,"abstract":"","PeriodicalId":14585,"journal":{"name":"Japanese journal of environmental toxicology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74656686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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