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The chimeric UreB, FliD and Omp18 proteins for a sensitive and specific diagnosis of Helicobacter pylori infections. 嵌合 UreB、FliD 和 Omp18 蛋白用于幽门螺旋杆菌感染的敏感性和特异性诊断。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15768
Hassan Seyyedhamzeh, Safar Farajnia, Mohammad Kargar, Behzad Baradaran, Farshid Kafilzadeh

Background and objectives: Helicobacter pylori is known as the main cause of gastrointestinal diseases including gastritis, gastric ulcer and stomach cancer. Serodiagnosis of H. pylori infection is a noninvasive and rapid method but the efficiency of this method is highly dependent to the antigens used. This study evaluated the efficacy of recombinant UreB-Omp18 and FliD for serodiagnosis of H. pylori infection.

Materials and methods: The genes encoding for fliD, ureB, and omp18 was amplified by PCR and cloned into pET-22b and pET-28a vectors. The constructs were expressed in E. coli BL21 and purified by affinity chromatography. The antigenic properties and diagnostic potential of the recombinant proteins were analysed by immunoblotting and ELISA, respectively.

Results: The recombinant UreB-Omp18 and FliD with molecular weights of 48 kDa and 25 kDa were observed on SDS-PAGE and purified by the Ni-NTA column. The ELISA results showed that the sensitivity and specificity of recombinant UreB-Omp18 protein in serodiagnosis of H. pylori infection were 89% and 83%, respectively. Also, the sensitivity and specificity of the recombinant FliD protein were calculated to be 91% and 76%, respectively.

Conclusion: The results indicated that the recombinant UreB-Omp18 and FliD could diagnose H. pylori infection with high sensitivity and specificity.

背景和目的:幽门螺杆菌是胃炎、胃溃疡和胃癌等胃肠道疾病的主要致病菌。幽门螺杆菌感染的血清诊断是一种非侵入性的快速方法,但这种方法的效率与所使用的抗原有很大关系。本研究评估了重组 UreB-Omp18 和 FliD 在幽门螺杆菌感染血清诊断中的有效性:通过 PCR 扩增 fliD、ureB 和 omp18 的编码基因,并将其克隆到 pET-22b 和 pET-28a 载体中。构建体在大肠杆菌 BL21 中表达,并通过亲和层析进行纯化。分别用免疫印迹法和酶联免疫吸附法分析了重组蛋白的抗原性和诊断潜力:结果:重组蛋白 UreB-Omp18 和 FliD 在 SDS-PAGE 上的分子量分别为 48 kDa 和 25 kDa,并经 Ni-NTA 柱纯化。ELISA 结果显示,重组 UreB-Omp18 蛋白在幽门螺杆菌感染血清诊断中的灵敏度和特异性分别为 89% 和 83%。重组 FliD 蛋白的敏感性和特异性分别为 91% 和 76%:结果表明,重组 UreB-Omp18 和 FliD 能以较高的灵敏度和特异性诊断幽门螺杆菌感染。
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引用次数: 0
Concomitant tuberculosis and aspergillosis in patients with COVID-19: a case report. COVID-19 患者合并结核病和曲霉菌病:病例报告。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15800
Elahe Sasani, Sadegh Khodavaisy, Mohammadreza Salehi, Sareh Bagheri-Josheghani, Mahsa Abdorahimi, Seyed Ali Dehghan Manshadi, Alireza Abdollahi, Amir Salami, Marjan Sohrabi, Arezoo Salami Khaneshan

Coexisting pulmonary aspergillosis and tuberculosis in a post-COVID-19 patient is rare. Here, we are going to report a case of combined pulmonary aspergillosis and tuberculosis in a 51-year-old female who was previously diagnosed with COVID-19 pneumonia. The patient was treated with voriconazole and anti-tuberculosis agents.

COVID-19 后患者同时患有肺曲霉菌病和结核病的情况非常罕见。在此,我们将报告一例合并肺曲霉菌病和肺结核的病例,患者是一名 51 岁的女性,曾被诊断为 COVID-19 肺炎。患者接受了伏立康唑和抗结核药物治疗。
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引用次数: 0
Designing the fusion protein of rotavirus VP8 and hepatitis A virus VP1 and evaluating the immunological response in BALB/c mice. 设计轮状病毒 VP8 和甲型肝炎病毒 VP1 的融合蛋白并评估 BALB/c 小鼠的免疫反应。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15797
Hassan Yarmohammadi, Mohammadreza Aghasadeghi, Abbas Akhavan Sepahi, Mojtaba Hamidi-Fard, Golnaz Bahramali

Background and objectives: Rotavirus and Hepatitis A virus are responsible for causing gastroenteritis and jaundice. The current vaccination approaches have proven insufficient, especially in low-income countries. In this study, we presented a novel dual-vaccine candidate that combines the rotavirus VP8 protein and the hepatitis A virus VP1.

Materials and methods: The VP8*-rotavirus+AAY+HAV-VP1 fusion protein was produced using an Escherichia coli expression system. The recombinant protein had a molecular weight of approximately 45.5 kDa and was purified through affinity chromatography. BALB/c mice were injected subcutaneously with the recombinant protein, VP1, VP8 and vaccines for rotavirus and hepatitis A virus, both with and without ALUM and M720 adjuvants. ELISA assays were used to measure total IgG, IgG1, IgG2, and short-term and long-term IL-5 and IFN-γ responses.

Results: The fusion protein, when combined with adjuvants, elicited significantly higher total IgG, IgG1, and IgG2 responses compared to VP1 and VP8 alone, as well as the rotavirus and hepatitis A vaccines. Furthermore, it induced a higher short-term IL-5 and IFN-γ response while demonstrating a higher long-term IL-5 response compared to the rotavirus and hepatitis A vaccines.

Conclusion: This study demonstrates that the VP8*-rotavirus+AAY+HAV-VP1 fusion protein is a promising dual vaccine candidate for immunization against hepatitis A and rotaviruses.

背景和目的:轮状病毒和甲型肝炎病毒可引起肠胃炎和黄疸。目前的疫苗接种方法已被证明是不够的,尤其是在低收入国家。在这项研究中,我们提出了一种新型双疫苗候选方案,它结合了轮状病毒 VP8 蛋白和甲型肝炎病毒 VP1:采用大肠杆菌表达系统生产出 VP8*-rotavirus+AAY+HAV-VP1 融合蛋白。重组蛋白的分子量约为 45.5 kDa,并通过亲和层析进行纯化。给 BALB/c 小鼠皮下注射重组蛋白、VP1、VP8 以及轮状病毒和甲型肝炎病毒疫苗(含或不含 ALUM 和 M720 佐剂)。采用 ELISA 方法测定总 IgG、IgG1、IgG2 以及短期和长期 IL-5 和 IFN-γ 反应:结果:与单独的 VP1 和 VP8 疫苗以及轮状病毒和甲型肝炎疫苗相比,融合蛋白与佐剂结合可引起明显更高的总 IgG、IgG1 和 IgG2 反应。此外,与轮状病毒疫苗和甲型肝炎疫苗相比,它能诱导更高的短期 IL-5 和 IFN-γ 反应,同时表现出更高的长期 IL-5 反应:本研究表明,VP8*-轮状病毒+AAY+HAV-VP1融合蛋白是一种很有前景的候选双联疫苗,可用于甲型肝炎和轮状病毒的免疫接种。
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引用次数: 0
Epidemiological and phylogenetic assessment of human respiratory syncytial virus among pediatric patients presenting acute respiratory infections in Shiraz, Iran during 2015-2016. 2015-2016 年伊朗设拉子急性呼吸道感染儿科患者中人类呼吸道合胞病毒的流行病学和系统发育评估。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15798
Saber Mojarrad, Nahid Tavakoli Movaghar, Fahime Edalat, Arash Letafati, Zahra Kargar Jahromi, Afagh Moattari

Background and objectives: The pediatric population worldwide bears a significant morbidity and death burden due to acute respiratory infections (ARIs). Human Orthopneumovirus, sometimes referred to as the Human Respiratory Syncytial Virus (HRSV), is one of the main causes of ARIs in infants. The main goal of this study was to identify the genetic diversity of HRSV strains that were circulating in the Iranian population at a certain time of year.

Materials and methods: Two hundred youngsters less than 12 years old with acute respiratory infections had samples taken from their throat and pharynx secretions. Then, external and hemi-nested PCR were employed, using specific primers targeting the G gene region to detect HRSV. Subsequently, nine randomly selected positive samples were subjected to sequencing. The results were then compared with reference strains cataloged in GeneBank, and phylogenetic tree was constructed using Chromes and MEGA7.

Results: Out of 200 samples, 34 were identified as containing HRSV. Subgroup A was predominant, accounting for 61.76% of cases, followed by subgroup BA (35.29%) and subgroup B (2.94%). Phylogenetic analysis revealed five samples associated with subtype B and four with genotype A. Genomic analysis showed three samples under the GA2 subgroup and one under GA1 for subtype A, and four samples in subgroup BA and one in GB2 for subtype B.

Conclusion: In this study, subgroup A strains, particularly genotype GA2, exhibited a higher prevalence compared to subgroup B strains during the specific period under investigation, shedding light on the genetic landscape of HRSV in this region.

背景和目的:急性呼吸道感染(ARIs)给全球儿科人口带来了巨大的发病和死亡负担。人类正肺炎病毒(有时也称为人类呼吸道合胞病毒(HRSV))是导致婴儿急性呼吸道感染的主要原因之一。本研究的主要目的是确定一年中某个时期在伊朗人群中流行的 HRSV 株系的遗传多样性:从 200 名患有急性呼吸道感染的 12 岁以下青少年的喉咙和咽部分泌物中提取样本。然后,使用针对 G 基因区的特异引物进行外部和半嵌合 PCR,以检测 HRSV。随后,对随机抽取的九个阳性样本进行了测序。然后将结果与基因库中的参考菌株进行比较,并使用 Chromes 和 MEGA7 构建系统发生树:结果:在 200 份样本中,有 34 份被鉴定为含有 HRSV。A亚群占主导地位,占病例总数的61.76%,其次是BA亚群(35.29%)和B亚群(2.94%)。基因组分析显示,A亚型有3个样本属于GA2亚组,1个属于GA1亚组,B亚型有4个样本属于BA亚组,1个属于GB2亚组:在本研究中,A亚群菌株,尤其是基因型为GA2的菌株,与B亚群菌株相比,在调查的特定时期表现出更高的流行率,从而揭示了该地区HRSV的基因状况。
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引用次数: 0
Oral inoculation of Enterococcus faecalis, DNA quantification and histopathological evaluation of gingival, heart and kidney tissue samples in rats. 给大鼠口服粪肠球菌,对牙龈、心脏和肾脏组织样本进行 DNA 定量和组织病理学评估。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15765
Fazle Khuda, Putri Ayu Jayusman, Badiah Baharin, Nur Najmi Mohamad Anuar, Anubhava Sharma, Nurrul Shaqinah Nasruddin

Background and objectives: Enterococcus faecalis is known as common pathogen for endodontic infections and cause secondary and refractory pulp periapical periodontitis. The bacteria can opportunistically colonize periodontal pockets and presents a possibility of infection developing in other organs. This research will investigate the dissemination of E. faecalis from the gingival tissue to the heart and kidney.

Materials and methods: Three groups were formed, consisting of twelve male Sprague Dawley rats: a control group designated as 0-day, and experimental groups labeled as 7-days and 14-days. Periodontitis induced by concurrent infection with sterile wire 0.2 mm insertion and E. faecalis inoculation is performed into the gingival sulcus located between the maxillary right 1st and 2nd molar teeth area. After euthanasia, tissue samples around the maxillary gingiva, maxillary jaw samples, kidney and heart tissues were obtained for quantitative Real-Time PCR assay and histopathological analysis.

Results: Results showed at 7-days, there was an upregulation of E. faecalis gene expression in the gingiva, heart, and kidney samples as well as infiltration of the inflammatory cells at 7-days post induction, which consequently decreased at 14-days.

Conclusion: Thus, the study suggests dissemination of E. faecalis from gingival tissue to the heart, kidney which could be probable link between periodontal disease, heart, and kidney disease.

背景和目的:众所周知,粪肠球菌是牙髓感染的常见病原体,可引起继发性和难治性牙髓根尖周炎。这种细菌可在牙周袋内伺机定植,并有可能发展为其他器官的感染。本研究将探讨粪大肠杆菌从牙龈组织向心脏和肾脏的传播:由 12 只雄性 Sprague Dawley 大鼠组成三组:对照组称为 0 天组,实验组称为 7 天组和 14 天组。在上颌右侧第 1 和第 2 磨牙区之间的龈沟中插入 0.2 毫米的无菌丝,同时接种粪大肠杆菌,诱发牙周炎。安乐死后,采集上颌龈周围组织样本、上颌颌骨样本、肾脏和心脏组织样本,进行实时定量 PCR 检测和组织病理学分析:结果:结果显示,诱导后 7 天,粪肠球菌基因在牙龈、心脏和肾脏样本中表达上调,炎症细胞浸润,14 天后炎症细胞浸润减少:因此,该研究表明,粪大肠杆菌从牙龈组织扩散到心脏和肾脏,这可能是牙周病、心脏和肾脏疾病之间的联系。
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引用次数: 0
Application of pulsed-field gel electrophoresis for molecular identification of pathogenic Leptospira species in Iran: a rapid and reliable method. 应用脉冲场凝胶电泳对伊朗的病原钩端螺旋体物种进行分子鉴定:一种快速可靠的方法。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15763
Pejvak Khaki, Mohsen Bagherpour, Mehdi Gharakhani, Maryam Sadat Soltani, Fereshteh Shahcheraghi, Vajihe Sadat Nikbin

Background and objectives: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira serovars. The genus Leptospira cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is precious of public health problems and epidemiological studies. Pulsed-field gel electrophoresis facilitates rapid identification of Leptospires to the serovar levels.

Materials and methods: In this study, we employed PFGE to evaluate 28 Leptospira isolates, with animal, human and environmental origin, obtained from Razi Vaccine and Serum Research Institute of Karaj, Iran. PFGE patterns of 28 Leptospira serovars were generated using the Not I restriction enzyme in comparison with the lambda ladder.

Results: Out of 28 serovars evaluated, we identified 22 different pulsed types, designated P1-P22. Out of 22 pulse groups, 3 were found to be a common type, but others were a single Type. Groups consisting of the common type were P3, P9, P14, and P16. The results showed that the discriminatory index of PFGE by Not I enzyme was 0.99, demonstrating heterogeneous differentiation among serovar members.

Conclusion: The PFGE methodology used in this study showed excellent interlaboratory report usability, rapid, reliable, enabling standardization and data sharing between laboratories.

背景和目的:钩端螺旋体病是一种由致病性钩端螺旋体血清型引起的人畜共患疾病。传统技术无法区分钩端螺旋体属。然而,确定致病性血清型是公共卫生问题和流行病学研究的关键。脉冲场凝胶电泳有助于快速鉴定钩端螺旋体的血清型:在这项研究中,我们采用脉冲场凝胶电泳对从伊朗卡拉季的拉齐疫苗和血清研究所分离的 28 株钩端螺旋体进行了评估,这些钩端螺旋体来自动物、人类和环境。使用 Not I 限制酶与 lambda 梯形图进行比较,得出了 28 个钩端螺旋体血清型的 PFGE 模式:结果:在所评估的 28 个血清型中,我们确定了 22 个不同的脉冲类型,命名为 P1-P22。在 22 个脉冲群中,发现有 3 个是共同类型,其他则是单一类型。由共同类型组成的组别为 P3、P9、P14 和 P16。结果显示,Not I 酶的 PFGE 鉴别指数为 0.99,表明血清成员之间存在异质性区分:结论:本研究中使用的 PFGE 方法在实验室间报告的可用性方面表现出色,快速、可靠,可实现实验室间的标准化和数据共享。
{"title":"Application of pulsed-field gel electrophoresis for molecular identification of pathogenic <i>Leptospira</i> species in Iran: a rapid and reliable method.","authors":"Pejvak Khaki, Mohsen Bagherpour, Mehdi Gharakhani, Maryam Sadat Soltani, Fereshteh Shahcheraghi, Vajihe Sadat Nikbin","doi":"10.18502/ijm.v16i3.15763","DOIUrl":"10.18502/ijm.v16i3.15763","url":null,"abstract":"<p><strong>Background and objectives: </strong>Leptospirosis is a zoonotic disease caused by pathogenic <i>Leptospira</i> serovars. The genus <i>Leptospira</i> cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is precious of public health problems and epidemiological studies. Pulsed-field gel electrophoresis facilitates rapid identification of <i>Leptospires</i> to the serovar levels.</p><p><strong>Materials and methods: </strong>In this study, we employed PFGE to evaluate 28 <i>Leptospira</i> isolates, with animal, human and environmental origin, obtained from Razi Vaccine and Serum Research Institute of Karaj, Iran. PFGE patterns of 28 <i>Leptospira</i> serovars were generated using the <i>Not</i> I restriction enzyme in comparison with the lambda ladder.</p><p><strong>Results: </strong>Out of 28 serovars evaluated, we identified 22 different pulsed types, designated P1-P22. Out of 22 pulse groups, 3 were found to be a common type, but others were a single Type. Groups consisting of the common type were P3, P9, P14, and P16. The results showed that the discriminatory index of PFGE by <i>Not</i> I enzyme was 0.99, demonstrating heterogeneous differentiation among serovar members.</p><p><strong>Conclusion: </strong>The PFGE methodology used in this study showed excellent interlaboratory report usability, rapid, reliable, enabling standardization and data sharing between laboratories.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Virulence genes and pulsed-field gel electrophoresis profiles of Shiga toxin-producing Escherichia coli isolated from different food samples and patients with acute diarrhea. 从不同食物样本和急性腹泻患者中分离出的产志贺毒素大肠杆菌的毒力基因和脉冲场凝胶电泳图谱。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15764
Mojtaba Bonyadian, Farzad Isvand Haidari, Masoud Sami

Background and objectives: Escherichia coli O157: H7 is one of the most important causes of hemorrhagic colitis, and hemolytic uremic syndrome. The present study aimed to isolate E. coli O157: H7 from foods and patients with hemorrhagic colitis, and identify Shiga toxin genes, phylogenetic comparison, and antibiotic resistance of the isolates.

Materials and methods: In total 400 samples, including patients stool and food were taken in Isfahan-Iran province. Phenotypic tests and PCR were performed to identify Shiga toxin-producing E. coli. The isolated strains were compared phylogenetically by PFGE. Agar disk diffusion was performed to identify the antibiotic resistance of the isolates.

Results: Totally, 5 isolates of fecal samples were E. coli O157, but only 2 isolates carried H7 gene. Also, 9 isolates of E. coli O157 were isolated from food samples that 3 isolates were E. coli O157: H7. The isolates carried stx1, stx2, hlyA and eaeA genes. Also, E. coli non-O157: H7 identified from samples that contained stx1, stx2, hlyA genes. The highest susceptibility to imipenem and the highest resistance to ampicillin and ciprofloxacin were observed. There was a similarity of 100% between the E. coli O157: H7 strains isolated from patients and raw milk and minced beef samples.

Conclusion: Serotypes other than the O157 of E. coli are more prevalent in patients and food. The E. coli O157: H7 isolates from patients had 100% genetic similarity with minced meat and cow milk isolates, which indicates cattle are the most important reservoir of this bacterium in Iran.

背景和目的:大肠杆菌O157:H7是导致出血性结肠炎和溶血性尿毒症的最重要原因之一。本研究旨在从食物和出血性结肠炎患者中分离大肠杆菌 O157: H7,并鉴定分离物的志贺毒素基因、系统发育比较和抗生素耐药性:在伊朗伊斯法罕省总共采集了 400 份样本,包括患者粪便和食物。通过表型检测和 PCR 鉴定产志贺毒素的大肠杆菌。通过 PFGE 对分离出的菌株进行系统发育比较。采用琼脂盘扩散法鉴定分离菌株的抗生素耐药性:结果:粪便样本中共分离出 5 株 O157 型大肠杆菌,但只有 2 株携带 H7 基因。此外,从食物样本中分离出 9 株 O157 型大肠杆菌,其中 3 株为 O157: H7 型大肠杆菌。这些分离物携带 stx1、stx2、hlyA 和 eaeA 基因。此外,还从含有 stx1、stx2 和 hlyA 基因的样本中鉴定出了非 O157: H7 型大肠杆菌。观察到对亚胺培南的敏感性最高,对氨苄西林和环丙沙星的耐药性最高。从患者、生牛奶和碎牛肉样本中分离出的大肠杆菌 O157: H7 菌株之间的相似度为 100%:结论:除 O157 型大肠杆菌外,其他血清型在患者和食物中更为普遍。从患者体内分离出的大肠杆菌 O157: H7 与碎肉和牛奶中分离出的大肠杆菌具有 100%的遗传相似性,这表明牛是伊朗最重要的大肠杆菌储库。
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引用次数: 0
Predictors of thirty-day mortality among patients with blood stream infection with WHO priority pathogens: single centre exploratory study from a referral teaching hospital in central India. 世界卫生组织重点病原体血流感染患者三十天死亡率的预测因素:印度中部一家转诊教学医院的单中心探索性研究。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15748
Akshit Budhiraja, Tadepalli Karuna, Farhan Khan, Shweta Kumar, Namitha Shaji, Ehsaas Bajaj, Shashank Purwar, Abhijit Pakhare, Rajnish Joshi, Saurabh Saigal, Sagar Khadanga

Background and objectives: Bloodstream infection (BSI) is defined by the presence of viable microorganisms in the bloodstream. BSI is one of the major causes of sepsis and subsequent adverse clinical outcomes all across the globe. The present study was undertaken to identify clinico-epidemio-microbiological variables associated with 30-day mortality in patients having BSI with WHO priority pathogens.

Materials and methods: The study was conducted at a public sector tertiary care institute in central India from April 2019 to March 2021. Blood samples collected from patients with clinical suspicion of sepsis, were processed by automated bacterial culture system and interpreted as per CLSI guidelines. Calculated sample size was 150. Data was analyzed by R software.

Results: Respiratory tract infection was the most common source (43.3%) of BSI, followed by the gastrointestinal (20%) and urinary tract (18.7%). Among the patients, 33% required invasive mechanical ventilation, and 31% required inotropes. Diabetes mellitus (DM) was the most common co-morbidity (34%). The incidence of multi-drug resistant organisms (MDRO) was 59.3%. Escherichia coli was the most commonly (24%) isolated organism, followed by Klebsiella pneumoniae (17.3%) and Acinetobacter baumannii (16%).

Conclusion: Higher age, higher qSOFA score / SIRS score / mean SOFA score at presentation had higher mortality. Use of mechanical ventilation and inotropes during treatment and isolation of critical category organisms of WPP and multi drug resistant organisms were independent 30-day mortality predictors.

背景和目的:血流感染(BSI)是指血液中存在可存活的微生物。在全球范围内,BSI 是导致败血症及随后不良临床结果的主要原因之一。本研究旨在确定与感染世界卫生组织重点病原体的 BSI 患者 30 天死亡率相关的临床流行病学-微生物学变量:本研究于 2019 年 4 月至 2021 年 3 月在印度中部一家公立三级医疗机构进行。从临床疑似败血症患者处采集的血液样本经自动细菌培养系统处理,并根据 CLSI 指南进行解释。计算样本量为 150 份。数据采用 R 软件进行分析:呼吸道感染是最常见的 BSI 来源(43.3%),其次是胃肠道感染(20%)和泌尿道感染(18.7%)。其中,33%的患者需要侵入性机械通气,31%的患者需要肌注。糖尿病是最常见的并发症(34%)。多重耐药菌(MDRO)的发病率为59.3%。大肠埃希菌是最常见的分离菌(24%),其次是肺炎克雷伯菌(17.3%)和鲍曼不动杆菌(16%):结论:年龄越大、qSOFA评分/SIRS评分/发病时平均SOFA评分越高,死亡率越高。在治疗过程中使用机械通气和肌注以及分离出 WPP 临界类别病原体和多重耐药病原体是预测 30 天死亡率的独立因素。
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引用次数: 0
Effect of collagen hydrogel containing Lavandula officinalis essential oil nanoemulsion in wound healing of infectious burn. 含有薰衣草精油纳米乳液的胶原蛋白水凝胶对感染性烧伤伤口愈合的影响。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15795
Hossein Teymouri, Mojgan Mohammadimehr, Mohammad Ahanjan, Somayeh Sheidaei, Majid Saeedi, Amir Mellati

Background and objectives: The main cause of mortality in burn patients is infection from burns. Drug-resistant bacteria are the main causes of wound infection, so alternative antibiotic therapies hold significant importance. The objective of this study was to examine the impact of a collagen hydrogel that contains a nanoemulsion of Lavandula essential oil on the healing process of infected burn wounds.

Materials and methods: In this experimental study, 20 rats were randomly divided after applying burns with a 10 mm diameter hot plate and infecting the wounds with multidrug-resistant Pseudomonas aeruginosa into four groups, including a positive control, a negative control, the first experiment (collagen hydrogel), and the second experiment (collagen hydrogel containing Lavandula essential oil nanoemulsion). On the 4th, 11th, and 18th days, tissue samples were taken for pathology studies. The important parameters in burn wound healing with hematoxylin and eosin and Masson's trichrome staining methods were investigated and scored according to Abramov's method.

Results: Based on the pathology findings, experimental groups 1 and 2 compared to the negative and positive control groups were effective in rat infection wound healing. The hydrogel scaffold in the experimental groups increased fibroblasts and angiogenesis compared to the control groups. Epithelization was noticed only in the hydrogel group containing nanoemulsion.

Conclusion: The study findings suggest that the use of collagen hydrogel with Lavandula essential oil nanoemulsion has potential as a wound dressing. This is because it has the potential to effectively promote healing and act as an antibacterial agent to prevent infections.

背景和目的:烧伤感染是烧伤患者死亡的主要原因。耐药菌是伤口感染的主要原因,因此替代抗生素疗法具有重要意义。本研究旨在探讨含有薰衣草精油纳米乳液的胶原蛋白水凝胶对感染性烧伤伤口愈合过程的影响:在本实验研究中,用直径为 10 毫米的热板烫伤大鼠并用耐多药铜绿假单胞菌感染伤口后,将 20 只大鼠随机分为四组,包括阳性对照组、阴性对照组、第一实验组(胶原蛋白水凝胶)和第二实验组(含薰衣草精油纳米乳液的胶原蛋白水凝胶)。第 4 天、第 11 天和第 18 天,采集组织样本进行病理学研究。用苏木精、伊红和马森三色染色法检测烧伤创面愈合的重要参数,并根据阿布拉莫夫法进行评分:根据病理结果,实验 1 组和 2 组与阴性对照组和阳性对照组相比,对大鼠感染伤口愈合有效。与对照组相比,实验组的水凝胶支架增加了成纤维细胞和血管生成。结论:研究结果表明,使用含有薰衣草精油纳米乳液的胶原蛋白水凝胶具有作为伤口敷料的潜力。这是因为它具有有效促进伤口愈合和作为抗菌剂预防感染的潜力。
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引用次数: 0
Evaluation of antibiotic resistance changes in Acinetobacter baumannii in the era of COVID-19 in Northern Iran. 评估 COVID-19 时代伊朗北部鲍曼不动杆菌的抗生素耐药性变化。
IF 1.3 Q4 MICROBIOLOGY Pub Date : 2024-06-01 DOI: 10.18502/ijm.v16i3.15762
Golnar Rahimzadeh, Reza Valadan, Shaghayegh Rezai, Mohammad Khosravi, Laleh Vahedi Larijani, Somayeh Sheidaei, Ebrahim Nemati Hevelaee, Faezeh Sadat Movahedi, Raha Rezai, Mohammad Sadegh Rezai

Background and objectives: During the coronavirus pandemic, the overuse of antibiotics to reduce coinfections and mortality may be contributing to the rise of antimicrobial resistance. In this study, we aim to investigate the antibiotic resistance changes of Acinetobacter baumannii post-COVID-19 pandemic in Northern Iran.

Materials and methods: The current study is a cross-sectional study. Between 2022 and 2023, 2190 clinical samples were collected from patients with healthcare-associated infections (HAIs) at four hospitals in Sari, which served as corona centers after the COVID-19 pandemic. Antimicrobial sensitivity was determined using standard broth macro-dilution, and resistance genes were detected using multiplex PCR.

Results: Based on the results co-amoxiclav had a resistance rate of 100%, while piperacillin/tazobactam showed the least resistance rate of 29.82%. In terms of GM MIC values, colistin was the most potent against multi-drug resistant isolates. The frequency of bla OXA-51 , ampC, aphA6, and bla NDM genes were 100%, 99.12%, 90.35%, and 69.30% respectively.

Conclusion: Our study revealed high multi-drug resistance rates. Piperacillin/tazobactam recommended for treating multi-drug resistant Acinetobacter baumannii infections in Northern Iran.

背景和目的:在冠状病毒大流行期间,为减少并发感染和死亡率而过度使用抗生素可能会导致抗生素耐药性的上升。在本研究中,我们旨在调查伊朗北部 COVID-19 大流行后鲍曼不动杆菌的抗生素耐药性变化:本研究为横断面研究。2022 年至 2023 年期间,从 COVID-19 大流行后作为日冕中心的萨里市四家医院的医源性感染(HAI)患者处收集了 2190 份临床样本。使用标准肉汤大稀释法测定抗菌药敏感性,并使用多重 PCR 检测耐药基因:结果显示,共阿莫西林的耐药率为 100%,而哌拉西林/他唑巴坦的耐药率最低,仅为 29.82%。从 GM MIC 值来看,可乐定对耐多药的分离株最有效。bla OXA-51、ampC、aphA6 和 bla NDM 基因的频率分别为 100%、99.12%、90.35% 和 69.30%:结论:我们的研究揭示了较高的多重耐药率。建议在伊朗北部治疗对多种药物耐药的鲍曼不动杆菌感染时使用哌拉西林/他唑巴坦。
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引用次数: 0
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Iranian Journal of Microbiology
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