{"title":"Characterization of the pull-up reduction feature on Data collection 4 in forensic STR typing","authors":"Shota Inokuchi, N. Mizuno, K. Sekiguchi","doi":"10.3408/jafst.787","DOIUrl":"https://doi.org/10.3408/jafst.787","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"31 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91497122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
10.3408 / jafst.794 ) The concealed information test ( CIT ) is a psychophysiological memory-detec-tion technique. The judgment to assess whether an examinee recognizes crime-relevant information is based on the diŠerence in autonomic responses between the crime-relevant information and the crime-irrelevant information. The present study examined the within-questionnaire consistency of the diŠerential physiological responses, which is a key point in the above judgment. The mock theft CIT data ob-tained from 167 participants were modeled into three possible models, and Bayesian estimation of parameters and model selection were performed. Results of model selection implied that participants in the encoding group were not clearly divided into high consistency responders and non-responders. Bayesian estimates of parameters revealed that the within-questionnaire consistency of the diŠerential physiological responses had diŠerent characteristics among the physiological measures. Most participants showed high consistency for heart rate and respiratory speed, while some participants show low consistency for skin conductance response, respiratory
{"title":"Within-questionnaire consistency of the differential physiological responses in a Concealed Information Test: hierarchical Bayesian approach","authors":"Yusuke Shibuya","doi":"10.3408/JAFST.794","DOIUrl":"https://doi.org/10.3408/JAFST.794","url":null,"abstract":"10.3408 / jafst.794 ) The concealed information test ( CIT ) is a psychophysiological memory-detec-tion technique. The judgment to assess whether an examinee recognizes crime-relevant information is based on the diŠerence in autonomic responses between the crime-relevant information and the crime-irrelevant information. The present study examined the within-questionnaire consistency of the diŠerential physiological responses, which is a key point in the above judgment. The mock theft CIT data ob-tained from 167 participants were modeled into three possible models, and Bayesian estimation of parameters and model selection were performed. Results of model selection implied that participants in the encoding group were not clearly divided into high consistency responders and non-responders. Bayesian estimates of parameters revealed that the within-questionnaire consistency of the diŠerential physiological responses had diŠerent characteristics among the physiological measures. Most participants showed high consistency for heart rate and respiratory speed, while some participants show low consistency for skin conductance response, respiratory","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82063219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of quantitative method for Δ9-THC in cannabis infused foods using GC-FID and QuEChERS","authors":"Katsumi Tachi, Kei Ieuji, Daisuke Kurakami, Yukari Tsumura","doi":"10.3408/jafst.817","DOIUrl":"https://doi.org/10.3408/jafst.817","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90776661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Tsujikawa, Yuki Okada, Hiroki Segawa, Tadashi Yamamuro, K. Kuwayama, T. Kanamori, Y. Iwata, H. Inoue
{"title":"Evaluation of test performance of Simon's reagent and Marquis reagent for so-called Ecstasy tablets","authors":"K. Tsujikawa, Yuki Okada, Hiroki Segawa, Tadashi Yamamuro, K. Kuwayama, T. Kanamori, Y. Iwata, H. Inoue","doi":"10.3408/jafst.791","DOIUrl":"https://doi.org/10.3408/jafst.791","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"120 2 1","pages":"103-113"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88755917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ricin, which is a proteinous toxin contained in castor beans, is one of the chemical warfare agents and strictly regulated by law. Regardless of the high toxicity, they have been used in some cases such as attempted murder cases due to the easy accessibility of castor beans. For these cases, detection of ricin is important for case control and proper remediation or decontamination. In this work, we have investigated the applicability of Bio-Threat Alert (BTA) test strips for the detection of ricin in beverages. By using BTA test strips, standard ricin protein in various beverages as low as 0.1 mg/mL could be detected by visual detection or by Guardian BTA Test Strip Reader. The calibration curves for ricin in tomato juice and cafe au lait were constructed using sample values obtained from Guardian BTA Test Strip Reader, which is the intensity of the color developed by the reaction of ricin and antibody. At high concentrations, the response seemed to be saturated. From the beverages added with mashed castor beans, ricin could also be detected by BTA test strips. Some beverages such as milk showed interfering behavior, but the reason seemed to not only come from the total protein amount but other factors.
蓖麻毒素是蓖麻中含有的一种蛋白质毒素，是一种受法律严格管制的化学战剂。尽管蓖麻有很高的毒性，但由于蓖麻很容易获得，它们被用于某些案件，如谋杀未遂案件。对于这些病例，检测蓖麻毒素对于病例控制和适当补救或去污非常重要。本研究探讨了生物威胁预警(BTA)试纸条在饮料中蓖麻毒素检测中的适用性。利用BTA试纸条，可通过目测或Guardian BTA试纸条读取器检测出低至0.1 mg/mL的各种饮料中的标准蓖麻蛋白。利用Guardian BTA Test Strip Reader获得的样品值(即蓖麻毒素与抗体反应后的显色强度)构建番茄汁和咖啡中蓖麻毒素的校准曲线。在高浓度时，反应似乎是饱和的。在添加蓖麻泥的饮料中，用BTA试纸也能检测出蓖麻毒素。一些饮料，如牛奶，表现出干扰行为，但原因似乎不仅来自蛋白质总量，还有其他因素。
{"title":"Detection of ricin in beverages using the Bio-Threat Alert test strips","authors":"Mai Otsuka, H. Miyaguchi","doi":"10.3408/jafst.818","DOIUrl":"https://doi.org/10.3408/jafst.818","url":null,"abstract":"Ricin, which is a proteinous toxin contained in castor beans, is one of the chemical warfare agents and strictly regulated by law. Regardless of the high toxicity, they have been used in some cases such as attempted murder cases due to the easy accessibility of castor beans. For these cases, detection of ricin is important for case control and proper remediation or decontamination. In this work, we have investigated the applicability of Bio-Threat Alert (BTA) test strips for the detection of ricin in beverages. By using BTA test strips, standard ricin protein in various beverages as low as 0.1 mg/mL could be detected by visual detection or by Guardian BTA Test Strip Reader. The calibration curves for ricin in tomato juice and cafe au lait were constructed using sample values obtained from Guardian BTA Test Strip Reader, which is the intensity of the color developed by the reaction of ricin and antibody. At high concentrations, the response seemed to be saturated. From the beverages added with mashed castor beans, ricin could also be detected by BTA test strips. Some beverages such as milk showed interfering behavior, but the reason seemed to not only come from the total protein amount but other factors.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79714864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Misato Wada, N. Shima, Tooru Kamata, S. Matsuta, Akari Ishikawa, Atsushi Nitta, Ryutaro Asai, Hidenao Kakehashi, Shihoko Fujii, K. Sasaki, H. Kamata, M. Katagi
{"title":"Urinary excretion profiles of etizolam and its main metabolites after a single oral dose","authors":"Misato Wada, N. Shima, Tooru Kamata, S. Matsuta, Akari Ishikawa, Atsushi Nitta, Ryutaro Asai, Hidenao Kakehashi, Shihoko Fujii, K. Sasaki, H. Kamata, M. Katagi","doi":"10.3408/JAFST.801","DOIUrl":"https://doi.org/10.3408/JAFST.801","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83342126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jun Ohta, M. Ohmura, Saki Minegishi, Koichi Sakurada
{"title":"Evaluation of a saliva presumptive test using the α-amylase assay kit","authors":"Jun Ohta, M. Ohmura, Saki Minegishi, Koichi Sakurada","doi":"10.3408/JAFST.802","DOIUrl":"https://doi.org/10.3408/JAFST.802","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78593684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DNA quantiˆcation is an important process in DNA typing. Today, quantitative real-time PCR ( qPCR ) targeting the autosomal D17Z1 region, which cannot distinguish between male and female DNA, is the main method for forensic human DNA quantiˆcation for the Japanese police. For more e‹cient testing Y-STR type, we examined the forensic evaluation ( sensitivity, repeatability, speciˆcity, inhibi-tion, comparison with commercial qPCR reagent and simulated mixture study ) of qPCR assays targeting Y-chromosomal Sex determining region Y ( SRY ) and DYZ5 regions. We evaluated the eŠectiveness of simple and practical male DNA quantiˆcation methods in male and female mixture samples. The sensitivity of SRY and DYZ5 assays were 0.015 ng / m L and 0.0015 ng / m L. Both assays could quantify male DNA in the presence of high concentrations of female DNA. The SRY assay had the same sensitivity as the commercial qPCR reagent, but had more resistant to inhibitors. The DYZ5 assay tended to have higher DNA concentration than the commercial qPCR reagent and the SRY assay, but could detect trace amounts of male DNA. The reason for this is that DYZ5 region is multi-copy locus and there are individual diŠerences in the copy number. The DYZ5 assay was considered to be one of the eŠective methods for proving the presence of male DNA in the sample. Therefore, we consider that these assays can select the regions according to purpose and utilized to forensic applications.
DNA定量是DNA分型的一个重要过程。目前，针对不能区分男性和女性DNA的常染色体D17Z1区域的实时定量PCR (qPCR)是日本警方法医人类DNA定量的主要方法。为了更有效地检测Y- str型，我们检查了针对Y染色体性别决定区Y (SRY)和DYZ5区域的qPCR检测的法医评估(灵敏度、重复性、特异性、抑制、与商业qPCR试剂的比较和模拟混合物研究)。我们对男女混合样品中简便实用的男性DNA定量方法eŠectiveness进行了评价。SRY和DYZ5检测方法的灵敏度分别为0.015 ng / m L和0.0015 ng / m L，两种检测方法均能在高浓度女性DNA存在的情况下定量男性DNA。SRY检测与商用qPCR试剂具有相同的敏感性，但对抑制剂具有更强的抗性。与商用qPCR试剂和SRY法相比，DYZ5法的DNA浓度更高，但可以检测到微量的男性DNA。这是因为DYZ5区域是多拷贝位点，拷贝数中存在个体diŠerences。DYZ5测定被认为是eŠective证明样品中存在男性DNA的方法之一。因此，我们认为这些分析可以根据目的选择区域并用于法医应用。
{"title":"Study of male DNA quantification methods in male-female mixture samples","authors":"Sachi Tanaka, Jun Ohta, Kazuki Yamazaki","doi":"10.3408/JAFST.795","DOIUrl":"https://doi.org/10.3408/JAFST.795","url":null,"abstract":"DNA quantiˆcation is an important process in DNA typing. Today, quantitative real-time PCR ( qPCR ) targeting the autosomal D17Z1 region, which cannot distinguish between male and female DNA, is the main method for forensic human DNA quantiˆcation for the Japanese police. For more e‹cient testing Y-STR type, we examined the forensic evaluation ( sensitivity, repeatability, speciˆcity, inhibi-tion, comparison with commercial qPCR reagent and simulated mixture study ) of qPCR assays targeting Y-chromosomal Sex determining region Y ( SRY ) and DYZ5 regions. We evaluated the eŠectiveness of simple and practical male DNA quantiˆcation methods in male and female mixture samples. The sensitivity of SRY and DYZ5 assays were 0.015 ng / m L and 0.0015 ng / m L. Both assays could quantify male DNA in the presence of high concentrations of female DNA. The SRY assay had the same sensitivity as the commercial qPCR reagent, but had more resistant to inhibitors. The DYZ5 assay tended to have higher DNA concentration than the commercial qPCR reagent and the SRY assay, but could detect trace amounts of male DNA. The reason for this is that DYZ5 region is multi-copy locus and there are individual diŠerences in the copy number. The DYZ5 assay was considered to be one of the eŠective methods for proving the presence of male DNA in the sample. Therefore, we consider that these assays can select the regions according to purpose and utilized to forensic applications.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90053487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sonoda, Ami Nagata, K. Tomonari, Takaaki Ono, Yoshito Tomisaka, Eiji Nishi
Recently, AXO Science released STK Sperm Tracker Lab ( STK sheet ) and STK Sperm Tracker SPRAY ( STK spray ) to identify human semen stains. In this study, we examined the STK sheet and STK spray for their speciˆcity to detect human semen, sensitivity, eŠects on DNA, and response to old semen samples. Additionally, STK sheet was examined for optimal crimping time depending on the type of fabric. STK spray was examined through assessing the eŠect of ultraviolet ( UV ) radiation after spraying. As a result of this study, STK sheet and STK spray were found to ex-hibit high speciˆcity for the detection of human semen. These reagents did not aŠect the yield of extracted DNA or its degradation. STK sheet and STK spray can detect semen up to 1 / 25 th and 1 / 100 th dilutions, respectively. Both STK sheet and STK spray can react with semen samples left at room temperature for over a year. We found that the crimping time on STK sheet diŠers depending on the type of fabric, and the exposure to UV radiation for an hour after using STK spray does not aŠect DNA degradation. Conclusively, both STK sheet and STK spray are suitable to identify the human semen stains.
{"title":"Establishment of the new semen identification method and the examination to practical introduction","authors":"A. Sonoda, Ami Nagata, K. Tomonari, Takaaki Ono, Yoshito Tomisaka, Eiji Nishi","doi":"10.3408/jafst.824","DOIUrl":"https://doi.org/10.3408/jafst.824","url":null,"abstract":"Recently, AXO Science released STK Sperm Tracker Lab ( STK sheet ) and STK Sperm Tracker SPRAY ( STK spray ) to identify human semen stains. In this study, we examined the STK sheet and STK spray for their speciˆcity to detect human semen, sensitivity, eŠects on DNA, and response to old semen samples. Additionally, STK sheet was examined for optimal crimping time depending on the type of fabric. STK spray was examined through assessing the eŠect of ultraviolet ( UV ) radiation after spraying. As a result of this study, STK sheet and STK spray were found to ex-hibit high speciˆcity for the detection of human semen. These reagents did not aŠect the yield of extracted DNA or its degradation. STK sheet and STK spray can detect semen up to 1 / 25 th and 1 / 100 th dilutions, respectively. Both STK sheet and STK spray can react with semen samples left at room temperature for over a year. We found that the crimping time on STK sheet diŠers depending on the type of fabric, and the exposure to UV radiation for an hour after using STK spray does not aŠect DNA degradation. Conclusively, both STK sheet and STK spray are suitable to identify the human semen stains.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80500033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maho Kawamura, Mitsuru Aizawa, Akihiro Nakamoto, A. Namera
{"title":"Full automation of sample pretreatment operations by supported liquid extraction for the determination of psychotropic drugs in blood","authors":"Maho Kawamura, Mitsuru Aizawa, Akihiro Nakamoto, A. Namera","doi":"10.3408/jafst.784","DOIUrl":"https://doi.org/10.3408/jafst.784","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89060031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：