Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, T. Akutsu
{"title":"Developmental validation of an ABO genotyping method using TaqMan multiplex real-time PCR","authors":"Ken Watanabe, Kochi Toyomane, Takayuki Yamagishi, T. Akutsu","doi":"10.3408/jafst.829","DOIUrl":"https://doi.org/10.3408/jafst.829","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85258307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prediction of pedestrian's head collision position by analysis of 56 accidents and mathematical model simulation","authors":"S. Ishii, Yasuo Ono","doi":"10.3408/jafst.807","DOIUrl":"https://doi.org/10.3408/jafst.807","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83537450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"LC/MS and GC/MS analysis of Δ9-tetrahydrocannabinol, Δ8-tetrahydrocannabinol and their metabolites","authors":"Yu Matsuura, K. Tsujikawa, Y. Iwata, Sayo M Ito","doi":"10.3408/jafst.836","DOIUrl":"https://doi.org/10.3408/jafst.836","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75734424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Matsuoka, M. Kasamatsu, Hiromi Itamiya, Yasuhiro Suzuki
It is necessary to extract ignitable liquid residue from ˆre debris samples at scenes of arson using gasoline or kerosene as accelerants. Because extraction is essential for ˆre debris analysis and greatly in‰uences the composition of ignitable liquid residues, analysts have to understand the features of extraction methods and their proper conditions. To this purpose, we tested MonoTrap as an extraction method and then analyzed extracts by conducting gas chromatography mass spectrometry (GC/MS). We evaluated the eŠects of adsorption temperature, time, desorbing solvents, sample concentration, and the presence of water vapor on the composition of extracts. The result suggested that MonoTrap can be used successfully for forensic purposes and provided its proper conditions for extraction.
{"title":"Forensic analysis of ignitable liquid using MonoTrap and its evaluation","authors":"I. Matsuoka, M. Kasamatsu, Hiromi Itamiya, Yasuhiro Suzuki","doi":"10.3408/jafst.828","DOIUrl":"https://doi.org/10.3408/jafst.828","url":null,"abstract":"It is necessary to extract ignitable liquid residue from ˆre debris samples at scenes of arson using gasoline or kerosene as accelerants. Because extraction is essential for ˆre debris analysis and greatly in‰uences the composition of ignitable liquid residues, analysts have to understand the features of extraction methods and their proper conditions. To this purpose, we tested MonoTrap as an extraction method and then analyzed extracts by conducting gas chromatography mass spectrometry (GC/MS). We evaluated the eŠects of adsorption temperature, time, desorbing solvents, sample concentration, and the presence of water vapor on the composition of extracts. The result suggested that MonoTrap can be used successfully for forensic purposes and provided its proper conditions for extraction.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90036504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Identiˆcation of unknown plant samples collected from crime scenes is carried out by morphological observation or plant DNA barcoding. However, DNA barcoding is rarely performed because the DNA typing performed by forensic science laboratories in Japanese police departments generally does not involve DNA sequencing techniques. Therefore, it is necessary to develop plant identiˆcation and discrimination methods involving DNA fragment analysis that can be performed in these laboratories. A total of 66 plant samples (Poaceae, Typhaceae, and Cyperaceae) were analyzed by examining 8 sets of consensus primer pairs in simple sequence repeat regions of the chloroplast genome. The DNA fragment lengths of the PCR ampliˆcation products were compared and classiˆed into 61 types. The results of this study may aid in the classiˆcation and identiˆcation of unknown plant samples.
{"title":"Use of chloroplast SSR regions to analyze DNA polymorphisms in Poaceae","authors":"Naoyuki Araki, Hitomi S. Kikkawa, Kouichiro Tsuge","doi":"10.3408/jafst.826","DOIUrl":"https://doi.org/10.3408/jafst.826","url":null,"abstract":"Identiˆcation of unknown plant samples collected from crime scenes is carried out by morphological observation or plant DNA barcoding. However, DNA barcoding is rarely performed because the DNA typing performed by forensic science laboratories in Japanese police departments generally does not involve DNA sequencing techniques. Therefore, it is necessary to develop plant identiˆcation and discrimination methods involving DNA fragment analysis that can be performed in these laboratories. A total of 66 plant samples (Poaceae, Typhaceae, and Cyperaceae) were analyzed by examining 8 sets of consensus primer pairs in simple sequence repeat regions of the chloroplast genome. The DNA fragment lengths of the PCR ampliˆcation products were compared and classiˆed into 61 types. The results of this study may aid in the classiˆcation and identiˆcation of unknown plant samples.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78928523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tadashi Yamamuro, Yuki Okada, Hiroki Segawa, K. Kuwayama, K. Tsujikawa, T. Kanamori, Y. Iwata
{"title":"Evaluation of a cannabis seed examination method without cultivation process","authors":"Tadashi Yamamuro, Yuki Okada, Hiroki Segawa, K. Kuwayama, K. Tsujikawa, T. Kanamori, Y. Iwata","doi":"10.3408/jafst.837","DOIUrl":"https://doi.org/10.3408/jafst.837","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90322400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The comparison of physiological responses by knowledge and those by auditory stimulus changes when asking questions in the concealed information tests","authors":"Natsu Todoriki, Natsumi Maeno, Shintaro Sugino, Mayu Kunishima, Yuko Yasuda, Risa Maeda, Ryuta Same, Taito Mori, Michiko Tsuneoka, Tokihiro Ogawa","doi":"10.3408/jafst.806","DOIUrl":"https://doi.org/10.3408/jafst.806","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87271381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Katagiri, Yukako Hayashi, Ryoya Tozaka, Y. Tsumura
{"title":"Development of a marijuana field test method using Fast Blue BB coloration and thin-layer chromatography (TLC)","authors":"N. Katagiri, Yukako Hayashi, Ryoya Tozaka, Y. Tsumura","doi":"10.3408/jafst.842","DOIUrl":"https://doi.org/10.3408/jafst.842","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78698386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daisuke Imoto, M. Honma, N. Akiba, Manato Hirabayashi, Shintaro Onozuka, Kimiya Akita, K. Kurosawa
{"title":"Development for geometric image analysis programs for forensic engineering examination","authors":"Daisuke Imoto, M. Honma, N. Akiba, Manato Hirabayashi, Shintaro Onozuka, Kimiya Akita, K. Kurosawa","doi":"10.3408/jafst.833","DOIUrl":"https://doi.org/10.3408/jafst.833","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73358419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The multiple shots from the close range to a vehicle had occurred, and seven entrance holes was observed on the side window of the vehicle. After the analysis of the residues collected around the holes, the part of the residues had included the speciˆc elements: lead, barium, and antimony. Based on the analysis results, the residues were concluded as GSR. However, the collected samples had included few numbers of the particle consisted of the speciˆc three components comparing to the one or two components. So, we arranged the ˆring experiment to clarify the reason why the less amount of three components particles was detected. The experimental results showed that the total amount of lead-containing particles had increased and the ratio of three components particles had decreased, with the shooting distance getting close. In this paper, we suggested the two points below for the identiˆcation of GSR collected from the target subjected to a close-range shot. (1) Analyze as many particles as possible using the automatic analysis function so as not to miss the three components particles. (2) Focus on not only the three components particles but also particles containing the one or two speciˆc elements to improve the reliability of the positive results.
{"title":"Identification case report of gunshot residues deposited on a vehicle window subjected to multiple shots at close range","authors":"Kazunari Shibasaki, Masaru Nakamura","doi":"10.3408/jafst.810","DOIUrl":"https://doi.org/10.3408/jafst.810","url":null,"abstract":"The multiple shots from the close range to a vehicle had occurred, and seven entrance holes was observed on the side window of the vehicle. After the analysis of the residues collected around the holes, the part of the residues had included the speciˆc elements: lead, barium, and antimony. Based on the analysis results, the residues were concluded as GSR. However, the collected samples had included few numbers of the particle consisted of the speciˆc three components comparing to the one or two components. So, we arranged the ˆring experiment to clarify the reason why the less amount of three components particles was detected. The experimental results showed that the total amount of lead-containing particles had increased and the ratio of three components particles had decreased, with the shooting distance getting close. In this paper, we suggested the two points below for the identiˆcation of GSR collected from the target subjected to a close-range shot. (1) Analyze as many particles as possible using the automatic analysis function so as not to miss the three components particles. (2) Focus on not only the three components particles but also particles containing the one or two speciˆc elements to improve the reliability of the positive results.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76551471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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