{"title":"Screening of paraquat and diquat in human body fluid by matrix assisted laser desorption/ionization time-of-flight mass spectrometry after solid-phase dispersive extraction","authors":"Yusuke Suzuki, Tsuyoshi Kaneko, Koichi Saito","doi":"10.3408/JAFST.743","DOIUrl":"https://doi.org/10.3408/JAFST.743","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76455161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
STAGE DOI: 10.3408 / jafst.747 ) We propose the analysis method of spur mark distance for discrimination of inkjet printers using a measuring microscope. The conventional method is the measurement of the pitch and the mutual distance of the spur marks which depend on the model of inkjet printers. Therefore, it is di‹cult to discriminate the same models of inkjet printers by the conventional method. First, we established the analysis method to obtain the spur mark distance indented by one rotation of spur gear and examined the diŠerence of the distances in a printer using correlation coe‹cients. Next, we applied this method to discriminate 60 inkjet printers of the same model. As a result, the correlation coe‹cients between the same printers approximated to 1. On the other hand, the correlation coe‹cients between the diŠerent printers were widely distributed in the range from 1 to 1. These results indicate that our proposed method can distinguish printers of the same model and be a useful tool for discrimination
{"title":"Discrimination of inkjet printers by measurement of spur marks using a measuring microscope","authors":"Masanao Shionaga, Mototsugu Suzuki, Hitomi Kudo","doi":"10.3408/JAFST.747","DOIUrl":"https://doi.org/10.3408/JAFST.747","url":null,"abstract":" STAGE DOI: 10.3408 / jafst.747 ) We propose the analysis method of spur mark distance for discrimination of inkjet printers using a measuring microscope. The conventional method is the measurement of the pitch and the mutual distance of the spur marks which depend on the model of inkjet printers. Therefore, it is di‹cult to discriminate the same models of inkjet printers by the conventional method. First, we established the analysis method to obtain the spur mark distance indented by one rotation of spur gear and examined the diŠerence of the distances in a printer using correlation coe‹cients. Next, we applied this method to discriminate 60 inkjet printers of the same model. As a result, the correlation coe‹cients between the same printers approximated to 1. On the other hand, the correlation coe‹cients between the diŠerent printers were widely distributed in the range from 1 to 1. These results indicate that our proposed method can distinguish printers of the same model and be a useful tool for discrimination","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87363080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
STAGE DOI: 10.3408 / jafst.754 ) Triacylglycerols ( TAG ) are composed of three esteriˆed fatty acids bound to a glycerol backbone and are the main components of several types of edible or cook-ing oils. An oil such as salad oil is occasionally used for a crime and is left in the crime scene as physical evidence. In criminal investigation, one of important evi-dence is to identify the kind of oil. Therefore, matrix assisted laser desorption ionization time of ‰ight mass spectrometry ( MALDI TOF MS ) was used for forensic discrimination of oils. In this study, pencil lead as a matrix was used. And this MALDI TOF MS with pencil lead technique can potentially
{"title":"Forensic Discrimination of Oils by Matrix Assisted Laser Desorption Ionization Time Of Flight Mass Spectrometry (MALDI-TOF-MS)","authors":"Tsuyoshi Kaneko, Yusuke Suzuki","doi":"10.3408/jafst.754","DOIUrl":"https://doi.org/10.3408/jafst.754","url":null,"abstract":" STAGE DOI: 10.3408 / jafst.754 ) Triacylglycerols ( TAG ) are composed of three esteriˆed fatty acids bound to a glycerol backbone and are the main components of several types of edible or cook-ing oils. An oil such as salad oil is occasionally used for a crime and is left in the crime scene as physical evidence. In criminal investigation, one of important evi-dence is to identify the kind of oil. Therefore, matrix assisted laser desorption ionization time of ‰ight mass spectrometry ( MALDI TOF MS ) was used for forensic discrimination of oils. In this study, pencil lead as a matrix was used. And this MALDI TOF MS with pencil lead technique can potentially","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75663507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Motorization produced tra‹c accidents and its con‰icts to be brought into the court of law. The ˆeld of accident reconstruction was born to cope with these litigations. Essentially, tra‹c accident is a crime of negligence. Punishment and insurance payment depend upon the assessment of the case. Vehicle speed plays an important role in the ratio of fault and plays an important role as a standard of fault authorization. An analysis method has been developed ever since. Now in Japan, tra‹c accident is punished with a special law independent from the existing penal code. Speed analysis has become a more important factor under this law. Therefore speed analysis has a long historical path and still plays an important ˆeld within forensic science. Fundamental method of speed analysis has been long based on the application of basic physics principles. Recent evolution of electrical devices brought another approach to the speed analysis. Images of CCTV, drive recorders, and vehicle mounted electrical data are oŠering a good chance to analyze vehicle speed. We would like to review the speed analysis of the traditional methods and overview the trend of recent methods of the new era.
{"title":"Trend of speed analysis in accident reconstruction","authors":"Kenji Koide, T. Kodama","doi":"10.3408/JAFST.R020","DOIUrl":"https://doi.org/10.3408/JAFST.R020","url":null,"abstract":"Motorization produced tra‹c accidents and its con‰icts to be brought into the court of law. The ˆeld of accident reconstruction was born to cope with these litigations. Essentially, tra‹c accident is a crime of negligence. Punishment and insurance payment depend upon the assessment of the case. Vehicle speed plays an important role in the ratio of fault and plays an important role as a standard of fault authorization. An analysis method has been developed ever since. Now in Japan, tra‹c accident is punished with a special law independent from the existing penal code. Speed analysis has become a more important factor under this law. Therefore speed analysis has a long historical path and still plays an important ˆeld within forensic science. Fundamental method of speed analysis has been long based on the application of basic physics principles. Recent evolution of electrical devices brought another approach to the speed analysis. Images of CCTV, drive recorders, and vehicle mounted electrical data are oŠering a good chance to analyze vehicle speed. We would like to review the speed analysis of the traditional methods and overview the trend of recent methods of the new era.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78222548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Five commercial DNA extraction kits for microbial DNA in soil were evaluated in terms of the extraction e‹ciencies of artiˆcially-spiked extracellular DNA in soil. Commercially-available puriˆed DNA derived from the sperm of Clupea harengus (Atlantic herring) was homogeneously premixed in blank soil samples and extracted using the kits. The spiked DNA in each of the extracts was quantitated by real-time PCR to evaluate extraction e‹ciency using a speciˆc primer set for the mitochondrial 16S rRNA gene of Clupea harengus. Only the Extrap Soil DNA Kit could extract the DNA from all types of soils spiked at 10 mg/g soil (vegetable garden soil 0.85, courtyard soil 0.30 and virgin andosol 0.027), whereas other kits (Nucleospin Soil, Power Soil DNA Isolation Kit, ISOIL and ISOIL for Beads Beating) could not extract the DNA larger than the quantitation limit (0.020) from the courtyard soil and the virgin andosol which exhibited high phosphate absorption coe‹cient. Skim milk concentration and bead-beating time using the Extrap Soil DNA Kit were optimized for 20 mg/g soil and 30 s, respectively. Signiˆcant PCR inhibition was not observed under the optimized condition.
对5种市售土壤微生物DNA提取试剂盒对土壤中人工加钉细胞外DNA的提取效果进行了评价。从大西洋鲱鱼(Clupea harengus)精子中提取的市售纯化DNA在空白土壤样品中均匀混合，并使用试剂盒提取。采用实时荧光定量PCR对各提取液中的加标DNA进行定量分析，以评价提取效果，采用的引物为Clupea harengus线粒体16S rRNA基因特异性引物。在10 mg/g土壤中，只有Extrap土壤DNA试剂盒能从所有类型的土壤(菜园土壤0.85、庭院土壤0.30、原生土0.027)中提取DNA，而其他试剂盒(Nucleospin土壤、Power土壤DNA分离试剂盒、ISOIL和ISOIL for Beads Beating)均不能从庭院土壤和具有高磷酸盐吸收率的原生土中提取出大于定量限(0.020)的DNA。使用Extrap Soil DNA Kit优化脱脂奶浓度和打珠时间，分别为20 mg/g土壤和30 s。在优化条件下，未观察到明显的PCR抑制作用。
{"title":"Evaluation of the commercial kits for the extraction of extracellular DNA in soil","authors":"H. Miyaguchi, Hiroaki Nakahara, R. Sugita","doi":"10.3408/JAFST.748","DOIUrl":"https://doi.org/10.3408/JAFST.748","url":null,"abstract":"Five commercial DNA extraction kits for microbial DNA in soil were evaluated in terms of the extraction e‹ciencies of artiˆcially-spiked extracellular DNA in soil. Commercially-available puriˆed DNA derived from the sperm of Clupea harengus (Atlantic herring) was homogeneously premixed in blank soil samples and extracted using the kits. The spiked DNA in each of the extracts was quantitated by real-time PCR to evaluate extraction e‹ciency using a speciˆc primer set for the mitochondrial 16S rRNA gene of Clupea harengus. Only the Extrap Soil DNA Kit could extract the DNA from all types of soils spiked at 10 mg/g soil (vegetable garden soil 0.85, courtyard soil 0.30 and virgin andosol 0.027), whereas other kits (Nucleospin Soil, Power Soil DNA Isolation Kit, ISOIL and ISOIL for Beads Beating) could not extract the DNA larger than the quantitation limit (0.020) from the courtyard soil and the virgin andosol which exhibited high phosphate absorption coe‹cient. Skim milk concentration and bead-beating time using the Extrap Soil DNA Kit were optimized for 20 mg/g soil and 30 s, respectively. Signiˆcant PCR inhibition was not observed under the optimized condition.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78087949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daisuke Imoto, K. Kurosawa, K. Tsuchiya, K. Kuroki, Manato Hirabayashi, N. Akiba, H. Kakuda
STAGE DOI: 10.3408 / jafst.745 ) Gait recognition is one of recently evolving techniques by which we can recog-nize individuals by one's gait. There are two major approaches; silhouette-based and model-based. In Japan, a method based on GEI ( Gait Energy Image ) , which is one of the silhouette-based approaches, is used for forensic purposes. Sometimes, it is a problem of silhouettes' variabilities in one person due to diŠerent clothing that les-sen recognition reliability under the GEI method. Here, we analyzed and evaluated the average error rates under clothing variation conditions using the method called Dynamic-features method, which we previously proposed. The Dynamic-features method was built inspired by previous studies of model-based gait recognition, which uses time-series of feature points and local shape features around the points automatically extracted from silhouette sequences. Before analysis, we roughly categorize whole data in the OU-ISIR gait database -treadmill dataset B-, which con-tains side-view data, into ˆve clothing categories in order to deal with realistic oŠ-line forensic situation, where we cannot strictly control utilization of dynamic properties of human's gait.
{"title":"Evaluation of gait recognition using dynamics of feature points and local shape features under clothing variation conditions","authors":"Daisuke Imoto, K. Kurosawa, K. Tsuchiya, K. Kuroki, Manato Hirabayashi, N. Akiba, H. Kakuda","doi":"10.3408/JAFST.745","DOIUrl":"https://doi.org/10.3408/JAFST.745","url":null,"abstract":" STAGE DOI: 10.3408 / jafst.745 ) Gait recognition is one of recently evolving techniques by which we can recog-nize individuals by one's gait. There are two major approaches; silhouette-based and model-based. In Japan, a method based on GEI ( Gait Energy Image ) , which is one of the silhouette-based approaches, is used for forensic purposes. Sometimes, it is a problem of silhouettes' variabilities in one person due to diŠerent clothing that les-sen recognition reliability under the GEI method. Here, we analyzed and evaluated the average error rates under clothing variation conditions using the method called Dynamic-features method, which we previously proposed. The Dynamic-features method was built inspired by previous studies of model-based gait recognition, which uses time-series of feature points and local shape features around the points automatically extracted from silhouette sequences. Before analysis, we roughly categorize whole data in the OU-ISIR gait database -treadmill dataset B-, which con-tains side-view data, into ˆve clothing categories in order to deal with realistic oŠ-line forensic situation, where we cannot strictly control utilization of dynamic properties of human's gait.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81571470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Izawa, Akira Furukawa, Sayaka Maruyama, H. Tsutsumi, T. Komuro
1Department of Special Needs Dentistry, Division of Hygiene and Oral Health, Showa University School of Dentistry 158, Hatanodai, Shinagawa-ku, Tokyo 1428555, Japan 2Forensic Dental Examiner Association for Aomori 1311, Aoyagi, Aomori 0300811, Japan 3Department of Legal Medicine, Nihon University School of Dentistry 1018310, Kanda-Surugadai, Chiyoda-ku, Tokyo 1018310, Japan 4Devision of Social Dentistry, Nihon University School of Dentistry 1018310, Kanda-Surugadai, Chiyoda-ku, Tokyo 1018310, Japan
{"title":"A case of personal identification based on the radiographic image of a remnant of a broken endodontic instrument and alveolar bone resorption","authors":"H. Izawa, Akira Furukawa, Sayaka Maruyama, H. Tsutsumi, T. Komuro","doi":"10.3408/JAFST.749","DOIUrl":"https://doi.org/10.3408/JAFST.749","url":null,"abstract":"1Department of Special Needs Dentistry, Division of Hygiene and Oral Health, Showa University School of Dentistry 158, Hatanodai, Shinagawa-ku, Tokyo 1428555, Japan 2Forensic Dental Examiner Association for Aomori 1311, Aoyagi, Aomori 0300811, Japan 3Department of Legal Medicine, Nihon University School of Dentistry 1018310, Kanda-Surugadai, Chiyoda-ku, Tokyo 1018310, Japan 4Devision of Social Dentistry, Nihon University School of Dentistry 1018310, Kanda-Surugadai, Chiyoda-ku, Tokyo 1018310, Japan","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"199 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76014390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasuhiro Suzuki, Takao Igawa, M. Kasamatsu, D. Kokubu, Atsushi Funatsuki
Applications of ICP-QMS/QMS using H2 as a reaction gas were investigated for the accurate and precise determination of Fe in glass fragments. The maximum signal to background ratio was calculated by dividing ion counts for the sample solution (50 ng mL-1 Fe in 0.8 mol L-1 HNO3) by that obtained for a blank solution (0.8 mol L-1 HNO3) at H2 gas ‰ow rates of 8 and 6 mL min-1 for m/z=54 and 56, respectively. The obtained values for 54Fe and 56Fe in a certiˆed reference glass material (NIST 612) under the optimized condition in the H2 gas mode showed a good match with the reference value (51±2 mg g-1) with a precision of <7.1 by RSD. The number of indistinguishable pairs among the 13 sheet glass samples manufactured using the same production line was limited to 12 of 78 by comparison of only Fe concentrations measured using the method developed herein, demonstrating the potential contribution of Fe analysis to enhanced evidence collection.
研究了以H2为反应气体的ICP-QMS/QMS对玻璃碎片中铁的精确测定。最大信本比是用样品溶液(50 ng mL-1 Fe在0.8 mol L-1 HNO3中)的离子计数除以空白溶液(0.8 mol L-1 HNO3)在H2气流速为8和6 mL min-1时(m/z=54和56)的离子计数计算得到的。在优化条件下，在H2气体模式下，在经过认证的参考玻璃材料(NIST 612)中得到的54Fe和56Fe值与参考值(51±2 mg g-1)吻合良好，RSD精度<7.1。在使用同一条生产线生产的13个平板玻璃样品中，仅通过使用本文开发的方法测量的铁浓度进行比较，无法区分的样品数量限制在78对中的12对，这表明铁分析对增强证据收集的潜在贡献。
{"title":"Forensic discrimination of automotive sheet glass manufactured in a same production line by quantitative analysis of iron using an ICP-QMS/QMS with an octapole reaction cell","authors":"Yasuhiro Suzuki, Takao Igawa, M. Kasamatsu, D. Kokubu, Atsushi Funatsuki","doi":"10.3408/JAFST.752","DOIUrl":"https://doi.org/10.3408/JAFST.752","url":null,"abstract":"Applications of ICP-QMS/QMS using H2 as a reaction gas were investigated for the accurate and precise determination of Fe in glass fragments. The maximum signal to background ratio was calculated by dividing ion counts for the sample solution (50 ng mL-1 Fe in 0.8 mol L-1 HNO3) by that obtained for a blank solution (0.8 mol L-1 HNO3) at H2 gas ‰ow rates of 8 and 6 mL min-1 for m/z=54 and 56, respectively. The obtained values for 54Fe and 56Fe in a certiˆed reference glass material (NIST 612) under the optimized condition in the H2 gas mode showed a good match with the reference value (51±2 mg g-1) with a precision of <7.1 by RSD. The number of indistinguishable pairs among the 13 sheet glass samples manufactured using the same production line was limited to 12 of 78 by comparison of only Fe concentrations measured using the method developed herein, demonstrating the potential contribution of Fe analysis to enhanced evidence collection.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81063810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hidenao Kakehashi, H. Kamata, Akari Ishikawa, Ryutaro Asai, Atsushi Nitta, Misato Wada, Shihoko Nakano, Syuntaro Matsuta, K. Sasaki, N. Shima, Tooru Kamata, H. Nishioka, A. Miki, M. Katagi
N-tert-Butoxycarbonyl-methamphetamine (t-BOCMA), a tert-butoxycarbonyl (t-BOC) derivative of methamphetamine (MA), which has recently been reported in several countries, was seized for the ˆrst time in Japan in 2017. It deprotected easily in an acidic condition to result in an illicit MA, and recently became a newly designated drug of the Pharmaceutical and Medical Device Act. For drug enforcement, the information of its properties was, therefore, strongly demanded. In this study, we synthesized the t-BOCMA standard, acquired various analytical data, and demonstrated its conversion to MA in high yield in the relatively moderate acidic condition (5 HCl methanol solution, 50°C). Also, the stability of t-BOCMA in simulated gastric juice (0.08 M HCl, 37°C) was explored by using GC/MS. As the result, 19 of t-BOCMA remained even after 120 min incubation, and the T1/2 was calculated to be 50 min. These suggest that the orally ingested t-BOCMA would be absorbed into blood in some degree without conversion to MA.
n -叔丁基羰基甲基苯丙胺(t-BOCMA)是甲基苯丙胺(MA)的叔丁基羰基(t-BOC)衍生物，最近在几个国家有报道，于2017年在日本首次被查获。它在酸性条件下容易失去保护，导致非法MA，最近成为《药品和医疗器械法》的新指定药物。因此，在禁毒执法方面，强烈要求提供有关其性质的资料。在本研究中，我们合成了t-BOCMA标准品，获得了各种分析数据，并证明了其在相对中等酸性条件下(5HCl甲醇溶液，50°C)高产率转化为MA。同时，采用GC/MS法考察了t-BOCMA在模拟胃液(0.08 M HCl, 37°C)中的稳定性。结果表明，t-BOCMA在培养120 min后仍有19存在，T1/2计算为50 min。这表明口服t-BOCMA在一定程度上被血液吸收而不转化为MA。
{"title":"Analyses of a methamphetamine precursor N-tert-butoxycarbonyl-methamphetamine (t-BOCMA) and its behavior in simulated human gastric juice","authors":"Hidenao Kakehashi, H. Kamata, Akari Ishikawa, Ryutaro Asai, Atsushi Nitta, Misato Wada, Shihoko Nakano, Syuntaro Matsuta, K. Sasaki, N. Shima, Tooru Kamata, H. Nishioka, A. Miki, M. Katagi","doi":"10.3408/jafst.753","DOIUrl":"https://doi.org/10.3408/jafst.753","url":null,"abstract":"N-tert-Butoxycarbonyl-methamphetamine (t-BOCMA), a tert-butoxycarbonyl (t-BOC) derivative of methamphetamine (MA), which has recently been reported in several countries, was seized for the ˆrst time in Japan in 2017. It deprotected easily in an acidic condition to result in an illicit MA, and recently became a newly designated drug of the Pharmaceutical and Medical Device Act. For drug enforcement, the information of its properties was, therefore, strongly demanded. In this study, we synthesized the t-BOCMA standard, acquired various analytical data, and demonstrated its conversion to MA in high yield in the relatively moderate acidic condition (5 HCl methanol solution, 50°C). Also, the stability of t-BOCMA in simulated gastric juice (0.08 M HCl, 37°C) was explored by using GC/MS. As the result, 19 of t-BOCMA remained even after 120 min incubation, and the T1/2 was calculated to be 50 min. These suggest that the orally ingested t-BOCMA would be absorbed into blood in some degree without conversion to MA.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80526295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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