{"title":"Simulation of arson fire with gasoline in the kitchen and development of a three-dimensional visualization model for evaluation of fire damage","authors":"Kousuke Tsuneyoshi, Kyoichiro Hirata, Ken'ichiro Suzuki, Yukimune Takahashi, Yuji Tobita, Atsushi Ueda, Shintaro Watanabe, Toshikazu Ichikawa, Masahiro Nakagawa, Takao Hagiwara, M. Honma, Katsuhiro Okamoto","doi":"10.3408/jafst.827","DOIUrl":"https://doi.org/10.3408/jafst.827","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73016973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Takahiro Kawakami, Atsushi Ishiba, Kenjiro Ito, S. Okuyama
{"title":"A new field-test for cocaine as an alternative to the Scott-test for use in criminal investigation fields","authors":"Takahiro Kawakami, Atsushi Ishiba, Kenjiro Ito, S. Okuyama","doi":"10.3408/jafst.812","DOIUrl":"https://doi.org/10.3408/jafst.812","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81148018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inspection of the relationship between the car speed and the visually recognizable distance of the person lying on the road in night driving","authors":"Shinichi Ishii, Yasuo Ono, Toshihiko Hayakawa, Yuko Shimizu, Shuta Hiratsuka","doi":"10.3408/jafst.832","DOIUrl":"https://doi.org/10.3408/jafst.832","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91543514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Personal identification based on the radiographic images of the inverted findings of maxillary third molar and supernumerary mesiodens","authors":"H. Tsutsumi, H. Izawa, Akira Furukawa, T. Komuro","doi":"10.3408/jafst.831","DOIUrl":"https://doi.org/10.3408/jafst.831","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89792634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Internal validation for analytical and stochastic thresholds in the GlobalFiler system","authors":"Shojiro Manabe, M. Hashiyada, Atsushi Akane","doi":"10.3408/jafst.845","DOIUrl":"https://doi.org/10.3408/jafst.845","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84227415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Classification of gel fuel by a combination of instrumental analyses","authors":"Munetsugu Moro","doi":"10.3408/jafst.835","DOIUrl":"https://doi.org/10.3408/jafst.835","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89702458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuko Suefusa, N. Otani, H. Wakuda, Kyoko Sato, M. Kuranari, N. Uemura
Blood acetaldehyde levels are often measured to elucidate individual diŠerences and pharmacokinetics of alcohol metabolism due to the gene polymorphisms of aldehyde ship ( R 2 ＝ 0.9994 ) in the range of 3 100 m M indicated strong results. The limit of detection and quantiˆcation was 1 m M and 3 m M, respectively. The intra- and inter-day precision were both below 13.0 and accuracy was in the range of － 8.5 to 2.5 . The extraction recoveries of acetaldehyde in human plasma ranged from 80.4 to 106.8 . The derivative was stable at 4 ° C for 2 days. When acetaldehyde was added in human plasma and derivatized with PQ, acetaldehyde could be quantiˆed by liquid chromatography-mass spectrometry. However, after drinking alcohol, acetaldehyde was measured in healthy subjects with ALDH2 1 / 2 , but not with ALDH2 1 / 1 . Acetaldehyde was di‹cult to detect in subjects with ALDH2 1 / 1 , as they are rapid metabolizers of acetaldehyde resulting in low concentrations of acetaldehyde. Acetaldehyde was detected in those subjects with ALDH2 1 / 2 , however, the measured acetaldehyde levels varied between the analytical methods.
通常测量血液中乙醛水平，以阐明由于醛ship基因多态性(r2 = 0.9994)在3100 m范围内的个体diŠerences和酒精代谢的药代动力学。检测限为1 m m，定量限为3 m m。日内和日间精度均低于13.0，精度在- 8.5~ 2.5之间。人血浆中乙醛的提取回收率为80.4~ 106.8。衍生物在4℃下稳定保存2天。将乙醛加入到人血浆中，用PQ衍生，可以用液相色谱-质谱法定量测定乙醛。然而，在饮酒后，健康受试者用ALDH21 /2测量乙醛，但不用ALDH21 /1。在ALDH2受试者中检测乙醛1 /1，因为他们是乙醛的快速代谢者，导致乙醛浓度低。在ALDH21 /2的受试者中检测到乙醛，然而，测量的乙醛水平在分析方法之间有所不同。
{"title":"Examination of method for the measurement of acetaldehyde in human plasma by using 9,10-phenanthrenequinone as a derivatizing agent","authors":"Yuko Suefusa, N. Otani, H. Wakuda, Kyoko Sato, M. Kuranari, N. Uemura","doi":"10.3408/JAFST.804","DOIUrl":"https://doi.org/10.3408/JAFST.804","url":null,"abstract":"Blood acetaldehyde levels are often measured to elucidate individual diŠerences and pharmacokinetics of alcohol metabolism due to the gene polymorphisms of aldehyde ship ( R 2 ＝ 0.9994 ) in the range of 3 100 m M indicated strong results. The limit of detection and quantiˆcation was 1 m M and 3 m M, respectively. The intra- and inter-day precision were both below 13.0 and accuracy was in the range of － 8.5 to 2.5 . The extraction recoveries of acetaldehyde in human plasma ranged from 80.4 to 106.8 . The derivative was stable at 4 ° C for 2 days. When acetaldehyde was added in human plasma and derivatized with PQ, acetaldehyde could be quantiˆed by liquid chromatography-mass spectrometry. However, after drinking alcohol, acetaldehyde was measured in healthy subjects with ALDH2 1 / 2 , but not with ALDH2 1 / 1 . Acetaldehyde was di‹cult to detect in subjects with ALDH2 1 / 1 , as they are rapid metabolizers of acetaldehyde resulting in low concentrations of acetaldehyde. Acetaldehyde was detected in those subjects with ALDH2 1 / 2 , however, the measured acetaldehyde levels varied between the analytical methods.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74320395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Matsuoka, Takao Hagiwara, M. Kasamatsu, Yasuhiro Suzuki, Masahiro Nakagawa, Toshikazu Ichikawa, Katsuhiro Okamoto, M. Honma
{"title":"Time-dependent changes in chemical composition and detectable period by GC/MS of petroleum-based liquid fuel residues from fire debris of various floor materials","authors":"I. Matsuoka, Takao Hagiwara, M. Kasamatsu, Yasuhiro Suzuki, Masahiro Nakagawa, Toshikazu Ichikawa, Katsuhiro Okamoto, M. Honma","doi":"10.3408/jafst.805","DOIUrl":"https://doi.org/10.3408/jafst.805","url":null,"abstract":"","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"387 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75528375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Takashi Fukagawa, Haruhiko Watahiki, Yusuke Mita, Tetsushi Kitayama, K. Fujii, N. Mizuno
Human DNA quantiˆcation is an important step for subsequent short tandem repeat (STR) analysis and interpretation because it can provide various information on the quantity and quality for the extracted DNA. In this study, we compared four commercially available quantiˆcation kits based on a TaqMan assay and an intercalating-dye based quantiˆcation kit. The following three points were investigated: 1) variations of the average peak heights among individuals when 1 ng of DNA, the amount being determined by each quantiˆcation kit, was ampliˆed using GlobalFiler and Yˆler Plus PCR Ampliˆcation Kits, 2) eŠect of the presence of a female DNA on the quantiˆcation of male DNA, and 3) relationship between ``Degradation Index'' and the STR electropherograms. The average peak heights generated using GlobalFiler showed less individual-dependent variations in the four TaqMan based kits than the intercalating-dye based kit. The average peak heights generated using Yˆler Plus showed similar variations among the three TaqMan based kits capable of male DNA quantiˆcation along with total human DNA. The presence of a large amount of female DNA had little eŠect on male DNA quantiˆcation in all the three kits. When highly degraded DNA samples were quantiˆed, the ``Degradation Indices'' diŠered signiˆcantly among three kits. It was probably due to the diŠerent amplicon sizes of the long fragment targets among kits. Before implementing a new human DNA quantiˆcation kit in casework, it is essential to understand the characteristics of the adopted quantiˆcation kit.
人类DNA定量是后续短串联重复(STR)分析和解释的重要步骤，因为它可以提供提取DNA的数量和质量的各种信息。在这项研究中，我们比较了四种市售的基于TaqMan检测的定量阳离子试剂盒和一种基于插层染料的定量阳离子试剂盒。我们研究了以下三点:1)使用GlobalFiler和Y - ler Plus PCR扩增试剂盒扩增1 ng DNA时个体间平均峰高的变化，每个定量检测试剂盒测定的数量为1 ng; 2)雄性DNA定量检测中女性DNA的存在eŠect; 3)“降解指数”与STR电泳之间的关系。使用GlobalFiler产生的平均峰高在四种基于TaqMan的试剂盒中显示出比基于插层染料的试剂盒更少的个体依赖性变化。使用Y + ler Plus生成的平均峰高在能够定量男性DNA和总人类DNA的三种TaqMan试剂盒中显示出相似的变化。在三个试剂盒中，大量雌性DNA的存在对雄性DNA的定量阳离子几乎没有eŠect影响。当对高度降解的DNA样本进行定量分析时，三个试剂盒的“降解指数”diŠered显著不同。这可能是由于试剂盒中长片段目标的diŠerent扩增子大小。在案例工作中实施新的人类DNA定量试剂盒之前，有必要了解所采用的定量试剂盒的特性。
{"title":"Comparison of human DNA quantification kits based on a TaqMan assay","authors":"Takashi Fukagawa, Haruhiko Watahiki, Yusuke Mita, Tetsushi Kitayama, K. Fujii, N. Mizuno","doi":"10.3408/jafst.821","DOIUrl":"https://doi.org/10.3408/jafst.821","url":null,"abstract":"Human DNA quantiˆcation is an important step for subsequent short tandem repeat (STR) analysis and interpretation because it can provide various information on the quantity and quality for the extracted DNA. In this study, we compared four commercially available quantiˆcation kits based on a TaqMan assay and an intercalating-dye based quantiˆcation kit. The following three points were investigated: 1) variations of the average peak heights among individuals when 1 ng of DNA, the amount being determined by each quantiˆcation kit, was ampliˆed using GlobalFiler and Yˆler Plus PCR Ampliˆcation Kits, 2) eŠect of the presence of a female DNA on the quantiˆcation of male DNA, and 3) relationship between ``Degradation Index'' and the STR electropherograms. The average peak heights generated using GlobalFiler showed less individual-dependent variations in the four TaqMan based kits than the intercalating-dye based kit. The average peak heights generated using Yˆler Plus showed similar variations among the three TaqMan based kits capable of male DNA quantiˆcation along with total human DNA. The presence of a large amount of female DNA had little eŠect on male DNA quantiˆcation in all the three kits. When highly degraded DNA samples were quantiˆed, the ``Degradation Indices'' diŠered signiˆcantly among three kits. It was probably due to the diŠerent amplicon sizes of the long fragment targets among kits. Before implementing a new human DNA quantiˆcation kit in casework, it is essential to understand the characteristics of the adopted quantiˆcation kit.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82536122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cable tie is one type of evidence submitted to forensic science laboratories, due to its potential as a tool used in crime. In this report, ten commercially available cable tie samples were analyzed and compared to study their potential for forensic discrimination. Samples were ˆrst evaluated through examination of their physical properties, shape, size, and traces from the mold. As a result of these examinations, 9 groups of the possible comparison pairs were discriminated. The chemical compo-sitions of the samples were subsequently characterized through the use of infrared spectroscopy and matrix assisted laser desorption ionization mass spectrometry. With this result, it was possible to discriminate the pair that could not originally be discriminated.
{"title":"Forensic discrimination of plastic cable ties","authors":"Tsuyoshi Kaneko, Y. Hanzawa","doi":"10.3408/JAFST.809","DOIUrl":"https://doi.org/10.3408/JAFST.809","url":null,"abstract":"Cable tie is one type of evidence submitted to forensic science laboratories, due to its potential as a tool used in crime. In this report, ten commercially available cable tie samples were analyzed and compared to study their potential for forensic discrimination. Samples were ˆrst evaluated through examination of their physical properties, shape, size, and traces from the mold. As a result of these examinations, 9 groups of the possible comparison pairs were discriminated. The chemical compo-sitions of the samples were subsequently characterized through the use of infrared spectroscopy and matrix assisted laser desorption ionization mass spectrometry. With this result, it was possible to discriminate the pair that could not originally be discriminated.","PeriodicalId":14709,"journal":{"name":"Japanese Journal of Forensic Science and Technology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88915867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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