Pub Date : 2024-03-19DOI: 10.35516/jjps.v17i1.1809
Alaa Sirhan, Yazan Alrashdan, Yousef Al-Ebini, L. Hassouneh, Tamara Ghrear, L. Abdulra’uf
Food poisoning from histamine, a biogenic amine formed due to the decarboxylation of histidine by bacteria in fish and fish products, has become a pivotal concern in food safety. This study measured the concentration of histamine in various fish products available in the Jordanian market, but manufactured in multiple countries, utilizing an Enzyme-Linked Immunosorbent Assay (ELISA). The ELISA kit and the protocol were provided by Veratox for histamine. Approximately 93.69% of the samples tested positive for the presence of histamine, with levels ranging between 0.317 and 230.41 mg/kg. Solely 0.9% of the samples exceeded the maximum permissible level established by the European Union (EU) and only 4.5% of the fish samples were free of histamine. The Principal Component Analysis (PCA) revealed that the type of fish was the most significant source of variability in histamine concentration, explaining 31.2% of the variability. Conversely, the sample weight accounted for the least variability (only 20.2%), implying that it has little or no effect on the concentration of histamine in the fish samples.
{"title":"Comparative Analysis of Histamine in Fresh and Processed Fish Sold in Jordanian Market","authors":"Alaa Sirhan, Yazan Alrashdan, Yousef Al-Ebini, L. Hassouneh, Tamara Ghrear, L. Abdulra’uf","doi":"10.35516/jjps.v17i1.1809","DOIUrl":"https://doi.org/10.35516/jjps.v17i1.1809","url":null,"abstract":"Food poisoning from histamine, a biogenic amine formed due to the decarboxylation of histidine by bacteria in fish and fish products, has become a pivotal concern in food safety. This study measured the concentration of histamine in various fish products available in the Jordanian market, but manufactured in multiple countries, utilizing an Enzyme-Linked Immunosorbent Assay (ELISA). The ELISA kit and the protocol were provided by Veratox for histamine. Approximately 93.69% of the samples tested positive for the presence of histamine, with levels ranging between 0.317 and 230.41 mg/kg. Solely 0.9% of the samples exceeded the maximum permissible level established by the European Union (EU) and only 4.5% of the fish samples were free of histamine. The Principal Component Analysis (PCA) revealed that the type of fish was the most significant source of variability in histamine concentration, explaining 31.2% of the variability. Conversely, the sample weight accounted for the least variability (only 20.2%), implying that it has little or no effect on the concentration of histamine in the fish samples.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140231196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-19DOI: 10.35516/jjps.v17i1.1692
I. Omoyayi, Süleyman Aşır, Abdullahi U. Ibrahim
Excipients are used in drug delivery systems as a means of effectively delivering drugs to their target site. Multi-criteria decision-making (MCDM) methods are tools for decision-making that consider multidimensional factors. Such methods are a comparative technology used in medicine that combines individual criteria into the total assessment of selected alternatives. This study aims to enhance the solubility and bioavailability of drugs through the application of MCDM-based excipient selection. By incorporating the Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE), various excipients can be evaluated and ranked based on their suitability for specific applications, considering parameters related to drug solubility and bioavailability. The results highlight the potential of cyclodextrins (net flow: 0.0023) and Eudragit polymers (net flow: 0.0016) as preferred options for drug carriers, while Poloxamer 188 (P188) (net flow: -0.0030) is identified as the least preferred option. This study demonstrates the effectiveness of the PROMETHEE method in improving the performance of poorly soluble and bioavailable drugs, ultimately contributing to the development of new drug delivery systems. The findings have significant implications for therapeutic outcomes in the treatment of diseases.
{"title":"Optimizing Drug Delivery Vehicle with Multi-Criteria Decision Making (MCDM) - Based Excipient Selection","authors":"I. Omoyayi, Süleyman Aşır, Abdullahi U. Ibrahim","doi":"10.35516/jjps.v17i1.1692","DOIUrl":"https://doi.org/10.35516/jjps.v17i1.1692","url":null,"abstract":"Excipients are used in drug delivery systems as a means of effectively delivering drugs to their target site. Multi-criteria decision-making (MCDM) methods are tools for decision-making that consider multidimensional factors. Such methods are a comparative technology used in medicine that combines individual criteria into the total assessment of selected alternatives. This study aims to enhance the solubility and bioavailability of drugs through the application of MCDM-based excipient selection. By incorporating the Preference Ranking Organization Method for Enrichment Evaluations (PROMETHEE), various excipients can be evaluated and ranked based on their suitability for specific applications, considering parameters related to drug solubility and bioavailability. The results highlight the potential of cyclodextrins (net flow: 0.0023) and Eudragit polymers (net flow: 0.0016) as preferred options for drug carriers, while Poloxamer 188 (P188) (net flow: -0.0030) is identified as the least preferred option. This study demonstrates the effectiveness of the PROMETHEE method in improving the performance of poorly soluble and bioavailable drugs, ultimately contributing to the development of new drug delivery systems. The findings have significant implications for therapeutic outcomes in the treatment of diseases.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-19DOI: 10.35516/jjps.v17i1.1808
O. Maslov, M. Komisarenko, S. Kolisnyk, Lyudmyla Derymedvid
The study aimed to identify the most abundant compounds in raspberry leaf extract via HPLC analysis, conduct theoretical and practical assessments of antioxidant and anti-inflammatory activities both in silico, in vitro, and in vivo, and evaluate the correlation between antioxidant and anti-inflammatory activities. Polyphenols were quantified using HPLC; molecular docking was carried out using AutoDockTools 1.5.6; antioxidant activity was ascertained via the potentiometric method; and anti-inflammatory activity was examined based on the carrageenan edema method. The extract was found to be rich in epicatechin (0.417%), (+)-catechin (0.501%), and ellagitannins (0.401%). The free energy of (+)-catechin and epicatechin was -8.40 and -7.20 respectively for the active sites of cyclooxygenase-2 (COX-2), and -6.60 and -7.11 for nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase). Notably, the antioxidant activity of the raspberry leaf extract was 1.43%, 1.04%, and 10.62% higher than that of green tea leaf extract for doses of 4.00, 2.00, 0.20 mg/mL, respectively. Treatment with the raspberry leaf extract at a dose of 13.0 mg/kg resulted in a significant decrease in edema after 1, 2, and 3 hours by 38.8%, 41.8%, and 48.8%, respectively, compared to the control group. The study demonstrated a correspondence between experimental and theoretical results in evaluating antioxidant and anti-inflammatory activities. Correlation analysis further substantiated that the anti-inflammatory action is dependent on antioxidant activity.
{"title":"Evaluation of Anti-Inflammatory, Antioxidant Activities and Molecular Docking Analysis of Rubus idaeus Leaf Extract","authors":"O. Maslov, M. Komisarenko, S. Kolisnyk, Lyudmyla Derymedvid","doi":"10.35516/jjps.v17i1.1808","DOIUrl":"https://doi.org/10.35516/jjps.v17i1.1808","url":null,"abstract":"The study aimed to identify the most abundant compounds in raspberry leaf extract via HPLC analysis, conduct theoretical and practical assessments of antioxidant and anti-inflammatory activities both in silico, in vitro, and in vivo, and evaluate the correlation between antioxidant and anti-inflammatory activities. Polyphenols were quantified using HPLC; molecular docking was carried out using AutoDockTools 1.5.6; antioxidant activity was ascertained via the potentiometric method; and anti-inflammatory activity was examined based on the carrageenan edema method. The extract was found to be rich in epicatechin (0.417%), (+)-catechin (0.501%), and ellagitannins (0.401%). The free energy of (+)-catechin and epicatechin was -8.40 and -7.20 respectively for the active sites of cyclooxygenase-2 (COX-2), and -6.60 and -7.11 for nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase). Notably, the antioxidant activity of the raspberry leaf extract was 1.43%, 1.04%, and 10.62% higher than that of green tea leaf extract for doses of 4.00, 2.00, 0.20 mg/mL, respectively. Treatment with the raspberry leaf extract at a dose of 13.0 mg/kg resulted in a significant decrease in edema after 1, 2, and 3 hours by 38.8%, 41.8%, and 48.8%, respectively, compared to the control group. The study demonstrated a correspondence between experimental and theoretical results in evaluating antioxidant and anti-inflammatory activities. Correlation analysis further substantiated that the anti-inflammatory action is dependent on antioxidant activity.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140230115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-19DOI: 10.35516/jjps.v17i2.1989
Safa M Abdulateef, S. Ibraheem, Humam S Hussein, B. Dheeb, B. M. Khashman, Dunya Muayed Ahmed, Khaled H Abu-Elteen, L. Abu-Qatouseh
Panax ginseng (PG), one of the most widely used herbal medicines, has demonstrated various beneficial effects such as anti-inflammatory, antioxidant, and anticancer impacts. Naturally occurring ginsenosides in the ginseng plant inhibit cell proliferation and significantly reduce liver damage induced by certain chemicals. Aflatoxin B1 (AFB1) is a primary mycotoxin due to its hepatotoxic, immunotoxic, and oncogenic effects in animal models and humans. In this study, we examined the effects of assorted doses of PG aqueous crude extract on the expression of matrix metalloproteinase 1 and 7 (MMP-1 and MMP-7) in the kidney, spleen, and liver of experimental AFB1-exposed mice, using immunohistochemistry (IHC). Mice were orally administered 6 mg/kg body weight (bw) of refined AFB1 (isolated and extracted from Aspergillus flavus, conc. 0.05 ppm) twice weekly for two weeks. We then compared the effects of three different doses (50, 100, and 150 mg/kg bw) of crude ginseng. We estimated the expression of MMP-1 and 7 in organs using IHC. We used the 6 mg/kg of purified AFB1, representing a 60% concentration, as a control group. IHC analysis showed that MMP (1 and 7) expression in the spleen, liver, and kidney of mice decreased after treatment with ginseng crude extract. MMP-1 expression was reduced in the liver by approximately 2.6 times, while the effectiveness in the MMP-1 reduction reached 9 and 8 times, respectively, in the spleen and kidney when treated with a higher dose of PG compared to the control. MMP-7 expression was reduced in the liver by approximately 13 times, while the reduction effectiveness fell to 2.3 and 5.6 times in the spleen and kidney when treated with a higher dose of PG compared to the control. The reduction in MMPs expression due to the effect of PG aqueous crude extract was observed to act against the effect of AFB1 on various living organs involved in AFB1 metabolism. IHC analysis indicated a more significant reduction efficiency observed in the expression of MMP-7 compared to both studied markers in the mice's liver.
{"title":"MMP-1 and MMP-7 Expression is Influenced by Ginsenosides in Mice Exposed to Aflatoxin B1: in vivo Study","authors":"Safa M Abdulateef, S. Ibraheem, Humam S Hussein, B. Dheeb, B. M. Khashman, Dunya Muayed Ahmed, Khaled H Abu-Elteen, L. Abu-Qatouseh","doi":"10.35516/jjps.v17i2.1989","DOIUrl":"https://doi.org/10.35516/jjps.v17i2.1989","url":null,"abstract":"Panax ginseng (PG), one of the most widely used herbal medicines, has demonstrated various beneficial effects such as anti-inflammatory, antioxidant, and anticancer impacts. Naturally occurring ginsenosides in the ginseng plant inhibit cell proliferation and significantly reduce liver damage induced by certain chemicals. Aflatoxin B1 (AFB1) is a primary mycotoxin due to its hepatotoxic, immunotoxic, and oncogenic effects in animal models and humans. In this study, we examined the effects of assorted doses of PG aqueous crude extract on the expression of matrix metalloproteinase 1 and 7 (MMP-1 and MMP-7) in the kidney, spleen, and liver of experimental AFB1-exposed mice, using immunohistochemistry (IHC). Mice were orally administered 6 mg/kg body weight (bw) of refined AFB1 (isolated and extracted from Aspergillus flavus, conc. 0.05 ppm) twice weekly for two weeks. We then compared the effects of three different doses (50, 100, and 150 mg/kg bw) of crude ginseng. We estimated the expression of MMP-1 and 7 in organs using IHC. We used the 6 mg/kg of purified AFB1, representing a 60% concentration, as a control group. IHC analysis showed that MMP (1 and 7) expression in the spleen, liver, and kidney of mice decreased after treatment with ginseng crude extract. MMP-1 expression was reduced in the liver by approximately 2.6 times, while the effectiveness in the MMP-1 reduction reached 9 and 8 times, respectively, in the spleen and kidney when treated with a higher dose of PG compared to the control. MMP-7 expression was reduced in the liver by approximately 13 times, while the reduction effectiveness fell to 2.3 and 5.6 times in the spleen and kidney when treated with a higher dose of PG compared to the control. The reduction in MMPs expression due to the effect of PG aqueous crude extract was observed to act against the effect of AFB1 on various living organs involved in AFB1 metabolism. IHC analysis indicated a more significant reduction efficiency observed in the expression of MMP-7 compared to both studied markers in the mice's liver.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140228300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-19DOI: 10.35516/jjps.v17i1.1606
Linda Hsien, Samir Srour
Objective: Polypharmacy among pediatric inpatients is common and exposes children to the risk of drug-drug interactions (DDIs). This study aimed to characterize potential DDIs (pDDIs) and their associated risk factors among pediatric inpatients. Methods: A cross-sectional study was conducted over six months at the University Children's Hospital in Damascus. A total of 575 children taking two drugs or more participated. pDDIs were checked using Lexi-Interact® software. pDDIs within risk category B (No action needed), C (Monitor therapy), D (Modify regimen), and X (Avoid combination) were included. Logistic regression was used to identify factors associated with pDDIs. Results: At least one pDDI was detected in 49.7% of children. Overall, 744 pDDIs were identified. The majority of pDDIs were within risk category C (71.6%), followed by D (14%), B (12.8%), and X (1.6%). The most common pDDIs were: aminoglycosides - penicillins (n=56), aminoglycosides - cephalosporins (n=27), and vitamin D analogs - calcium salts (n=23). The number of prescribed drugs and nervous system drugs were significantly associated with the presence of pDDIs. Conclusion: pDDIs among pediatric inpatients were prevalent. The majority of the pDDIs were within risk category C, which necessitates therapy monitoring and necessary action to avoid adverse consequences.
{"title":"Potential Drug-Drug Interactions and their Associated Factors at the University Children's Hospital in Syria: A Cross-Sectional Study","authors":"Linda Hsien, Samir Srour","doi":"10.35516/jjps.v17i1.1606","DOIUrl":"https://doi.org/10.35516/jjps.v17i1.1606","url":null,"abstract":"Objective: Polypharmacy among pediatric inpatients is common and exposes children to the risk of drug-drug interactions (DDIs). This study aimed to characterize potential DDIs (pDDIs) and their associated risk factors among pediatric inpatients.\u0000Methods: A cross-sectional study was conducted over six months at the University Children's Hospital in Damascus. A total of 575 children taking two drugs or more participated. pDDIs were checked using Lexi-Interact® software. pDDIs within risk category B (No action needed), C (Monitor therapy), D (Modify regimen), and X (Avoid combination) were included. Logistic regression was used to identify factors associated with pDDIs.\u0000Results: At least one pDDI was detected in 49.7% of children. Overall, 744 pDDIs were identified. The majority of pDDIs were within risk category C (71.6%), followed by D (14%), B (12.8%), and X (1.6%). The most common pDDIs were: aminoglycosides - penicillins (n=56), aminoglycosides - cephalosporins (n=27), and vitamin D analogs - calcium salts (n=23). The number of prescribed drugs and nervous system drugs were significantly associated with the presence of pDDIs.\u0000Conclusion: pDDIs among pediatric inpatients were prevalent. The majority of the pDDIs were within risk category C, which necessitates therapy monitoring and necessary action to avoid adverse consequences.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140229962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.35516/jjps.v16i4.1789
Tasneem Basheer Ali, Huda Yousef Almomani, F. Al-Tarawneh, M. Alwadi, Ahmad Shaher Suliman
Objective: This cross-sectional study aims to assess physicians' knowledge regarding theophylline drug and other related characteristics in Jordan. Materials and Method: The study was conducted prospectively among physicians in Jordan. Physicians were interviewed using an online questionnaire consisting of two sections. The first section included demographics and other relevant characteristics, while the second section comprised questions about theophylline drug. Results: A total of 385 participants completed the questionnaire. The majority of participants knew that theophylline is used in clinical practice as a bronchodilator (75.6%). Nearly 39% of participants knew that theophylline can be administered orally and intravenously. The largest share of participants (76.1%) did not know that theophylline dosage is calculated based on ideal body weight. Sixty percent of participants knew that theophylline use was not contraindicated during pregnancy. On the other hand, only 27.3% knew that theophylline use was not contraindicated during breastfeeding. The majority of participants (76.1%) had an overall intermediate knowledge of theophylline. Conclusions: It was noted that physicians had an overall intermediate knowledge of theophylline. Physicians demonstrated unsatisfactory knowledge about theophylline’s indications, clinical use, administration, adverse effects, and other related aspects. These findings highlight the need for educational interventions and training programs to improve physicians’ knowledge of theophylline and enhance its effective and safe use in clinical practice.
{"title":"Physicians' Knowledge of Theophylline Use: A Cross-Sectional Study from Jordan","authors":"Tasneem Basheer Ali, Huda Yousef Almomani, F. Al-Tarawneh, M. Alwadi, Ahmad Shaher Suliman","doi":"10.35516/jjps.v16i4.1789","DOIUrl":"https://doi.org/10.35516/jjps.v16i4.1789","url":null,"abstract":"Objective: This cross-sectional study aims to assess physicians' knowledge regarding theophylline drug and other related characteristics in Jordan. Materials and Method: The study was conducted prospectively among physicians in Jordan. Physicians were interviewed using an online questionnaire consisting of two sections. The first section included demographics and other relevant characteristics, while the second section comprised questions about theophylline drug. Results: A total of 385 participants completed the questionnaire. The majority of participants knew that theophylline is used in clinical practice as a bronchodilator (75.6%). Nearly 39% of participants knew that theophylline can be administered orally and intravenously. The largest share of participants (76.1%) did not know that theophylline dosage is calculated based on ideal body weight. Sixty percent of participants knew that theophylline use was not contraindicated during pregnancy. On the other hand, only 27.3% knew that theophylline use was not contraindicated during breastfeeding. The majority of participants (76.1%) had an overall intermediate knowledge of theophylline. Conclusions: It was noted that physicians had an overall intermediate knowledge of theophylline. Physicians demonstrated unsatisfactory knowledge about theophylline’s indications, clinical use, administration, adverse effects, and other related aspects. These findings highlight the need for educational interventions and training programs to improve physicians’ knowledge of theophylline and enhance its effective and safe use in clinical practice.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139157721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.35516/jjps.v16i4.1128
O. Ibukun, E. Uhunmwangho, Iyanuoluwa Ademola, Nisi-Dominus Olokor, Oluwasina Akinnaso
This study evaluated the anti-obesity properties of the methanol extract of Zingiber officinale leaves in Wistar rats. Thirty male rats were distributed into five groups, with six rats in each group, and different groups were treated with a normal fat diet (NFD), high-fat diet (HFD), HFD + orlistat (20 mg/kg) p.o, HFD + Zingiber officinale (200 mg/kg) p.o, and HFD + Zingiber officinale (400 mg/kg) p.o for fifty-six days. After all administrations, the animals were sacrificed by cervical dislocation, and various biochemical analyses were carried out. Results showed that there was a significant decrease (p < 0.05) in body weight and adiposity in the Zingiber officinale, NFD, and orlistat groups compared to the HFD control. However, there was no significant difference in the body weights of rats in the Zingiber officinale groups compared to the NFD control and orlistat groups. Furthermore, rats in the Zingiber officinale groups had normal lipid concentrations, antioxidant status, adipokines, cytokines, liver, kidney, and cardiac function parameters that were comparable to orlistat and normal control but in contrast with the HFD control. Findings from the study suggest that Zingiber officinale leaves have significant anti-obesity, antioxidant, and anti-inflammatory properties.
{"title":"Methanol Leaves Extract of Zingiber officinale (Roscoe) exhibited Anti-Obesity Effect in Wistar Rats Fed with a High Fat Diet","authors":"O. Ibukun, E. Uhunmwangho, Iyanuoluwa Ademola, Nisi-Dominus Olokor, Oluwasina Akinnaso","doi":"10.35516/jjps.v16i4.1128","DOIUrl":"https://doi.org/10.35516/jjps.v16i4.1128","url":null,"abstract":"This study evaluated the anti-obesity properties of the methanol extract of Zingiber officinale leaves in Wistar rats. Thirty male rats were distributed into five groups, with six rats in each group, and different groups were treated with a normal fat diet (NFD), high-fat diet (HFD), HFD + orlistat (20 mg/kg) p.o, HFD + Zingiber officinale (200 mg/kg) p.o, and HFD + Zingiber officinale (400 mg/kg) p.o for fifty-six days. After all administrations, the animals were sacrificed by cervical dislocation, and various biochemical analyses were carried out. Results showed that there was a significant decrease (p < 0.05) in body weight and adiposity in the Zingiber officinale, NFD, and orlistat groups compared to the HFD control. However, there was no significant difference in the body weights of rats in the Zingiber officinale groups compared to the NFD control and orlistat groups. Furthermore, rats in the Zingiber officinale groups had normal lipid concentrations, antioxidant status, adipokines, cytokines, liver, kidney, and cardiac function parameters that were comparable to orlistat and normal control but in contrast with the HFD control. Findings from the study suggest that Zingiber officinale leaves have significant anti-obesity, antioxidant, and anti-inflammatory properties.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139157958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-25DOI: 10.35516/jjps.v16i4.1027
Tegar Achsendo, T. A. Yuniarta, Gede Ari Sumartha, T. M. Fakih, Rosita Handayani, Dwi Syah, Fitra Ramadhan
Objectives: This study aimed to identify novel antimalarial compounds based on allosteric inhibitor of prolyl-tRNA synthetase using hierarchical virtual screening. Materials and Methods: Pharmacophore model was designed initially, based on the structure-activity relationships data between several pyrazole-urea analogues and their IC50 enzymatic value. The model obtained was applied to screen ZINC15 database, after which followed by drug-likeness, toxicophore, and PAINS filter. The hit compounds were docked against P. falciparum prolyl-tRNA synthetase enzyme, using validated docking method. The resulting docking poses were ranked based on the docking score and re-evaluated based on the pharmacophore criteria. Top five compounds were obtained from this step and then evaluated using molecular dynamics simulation to verify its stability and hydrogen bond dynamics over 50 nanoseconds. MM-PBSA analysis was also performed to estimate their binding free energy. Ultimately, their potential bioactivity as antimalarial candidates have been verified against 3D7 strain. Results: The results showed that all five compounds obtained from virtual screening possess micromolar potency in vitro. Two compounds (ZINC 1029449 and ZINC1029453), yield high antimalarial activity (0.44 and 0.72 μM, respectively) Conclusions: Overall, the virtual screening approach has successfully produced lead compounds which can be further optimized to be antimalarial agents.
{"title":"Discovery of Potential Prolyl-tRNA Synthetase Allosteric Inhibitor Through Virtual Screening and In Vitro Assay against Plasmodium falciparum","authors":"Tegar Achsendo, T. A. Yuniarta, Gede Ari Sumartha, T. M. Fakih, Rosita Handayani, Dwi Syah, Fitra Ramadhan","doi":"10.35516/jjps.v16i4.1027","DOIUrl":"https://doi.org/10.35516/jjps.v16i4.1027","url":null,"abstract":"Objectives: This study aimed to identify novel antimalarial compounds based on allosteric inhibitor of prolyl-tRNA synthetase using hierarchical virtual screening. Materials and Methods: Pharmacophore model was designed initially, based on the structure-activity relationships data between several pyrazole-urea analogues and their IC50 enzymatic value. The model obtained was applied to screen ZINC15 database, after which followed by drug-likeness, toxicophore, and PAINS filter. The hit compounds were docked against P. falciparum prolyl-tRNA synthetase enzyme, using validated docking method. The resulting docking poses were ranked based on the docking score and re-evaluated based on the pharmacophore criteria. Top five compounds were obtained from this step and then evaluated using molecular dynamics simulation to verify its stability and hydrogen bond dynamics over 50 nanoseconds. MM-PBSA analysis was also performed to estimate their binding free energy. Ultimately, their potential bioactivity as antimalarial candidates have been verified against 3D7 strain. Results: The results showed that all five compounds obtained from virtual screening possess micromolar potency in vitro. Two compounds (ZINC 1029449 and ZINC1029453), yield high antimalarial activity (0.44 and 0.72 μM, respectively) Conclusions: Overall, the virtual screening approach has successfully produced lead compounds which can be further optimized to be antimalarial agents.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139159270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The family Fabaceae is the third-largest flowering plant family, and the genus Desmodium has exhibited a wide range of biological activities and a variety of chemical constituents. In the present study, different extracts of Desmodium tortuosum were evaluated for their cytotoxic and antioxidant activities, as well as their total phenolic content (TPC). The antioxidant activities were estimated using the 1,1'-diphenyl-2-picraylhydrazyl free radical (DPPH), while the cytotoxic activity was evaluated via the brine shrimp lethality test (BSLT). The antioxidant activity results revealed that the DPPH radical scavenging activity (SC50) ranged from 1.12 to 61.22 µg/ml with respect to ascorbic acid (SC50 = 7.45 µg/ml). Among all tested fractions, 90% methanol was the most active. On the other hand, the cytotoxic activities were arranged as follows: n-BuOH (LC50 = 310), EtOAc (LC50 = 350), and 70% methanol (LC50 = 380). High-Performance Liquid Chromatography-Fingerprint analyses were used to determine the chemical composition and relative proportions of phenolic compounds. GC-MS analysis indicated the presence of fatty acids and other compounds. The major identified compounds were Benzene (1-butyloctyl) (11.88%) and Himachalene (11.08%) for the ethyl acetate extract and 10-Undecenoic acid, methyl ester (25.50%) for unsaponifiable matter.
{"title":"Cytotoxicity, Antioxidant Activities, GC-MS and HPLC Fingerprint Analyses of Different Extracts of Desmodium tortuosum (Sw.) DC","authors":"Maha Elshazly, Fatma A Hamada, L. Refahy","doi":"10.35516/jjps.v16i4.930","DOIUrl":"https://doi.org/10.35516/jjps.v16i4.930","url":null,"abstract":"The family Fabaceae is the third-largest flowering plant family, and the genus Desmodium has exhibited a wide range of biological activities and a variety of chemical constituents. In the present study, different extracts of Desmodium tortuosum were evaluated for their cytotoxic and antioxidant activities, as well as their total phenolic content (TPC). The antioxidant activities were estimated using the 1,1'-diphenyl-2-picraylhydrazyl free radical (DPPH), while the cytotoxic activity was evaluated via the brine shrimp lethality test (BSLT). The antioxidant activity results revealed that the DPPH radical scavenging activity (SC50) ranged from 1.12 to 61.22 µg/ml with respect to ascorbic acid (SC50 = 7.45 µg/ml). Among all tested fractions, 90% methanol was the most active. On the other hand, the cytotoxic activities were arranged as follows: n-BuOH (LC50 = 310), EtOAc (LC50 = 350), and 70% methanol (LC50 = 380). High-Performance Liquid Chromatography-Fingerprint analyses were used to determine the chemical composition and relative proportions of phenolic compounds. GC-MS analysis indicated the presence of fatty acids and other compounds. The major identified compounds were Benzene (1-butyloctyl) (11.88%) and Himachalene (11.08%) for the ethyl acetate extract and 10-Undecenoic acid, methyl ester (25.50%) for unsaponifiable matter.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
صُنعت جسيمات أوكسيد المغنيزيوم النّانويّة والمايكرونية بواسطة صبار التين الشوكي وتم تقييم فعاليتها المضادة للفطور المسببة للأمراض الهامة عند البشر كمبيضات البيض والرشاشيات السّوداء. وتم توصيف الجسيمات الناتجة بـِ UV–Vis، FTIR، DLS، EDX، .FESEMأظهر تحليل مطيافية الأشعة المرئية وفوق البنفسجية قمة عند 300 نانومتر، وبيّن اختبار الأشعة تحت الحمراء أن المستقلبات الحيوية في النبات تلعب دورًا مهمًا في إرجاع أيونات المعدن ثم نموها لتعطي في النهاية جسيماتٍ نانويّة، كما يبين التحليل قمة قريبة من 400 تعود للرابطة Mg-O-Mg. يؤكد تحليل الـ EDX وجود جسيمات أوكسيد المغنيزيوم. تراوحت أبعاد جسيمات أوكسيد المغنيزيوم النّانويّة ذات الشكل الكروي بين 15.5 و78.01 نانومتر (بمتوسط 42.28 نانومتر)، أما أبعاد الجسيمات المايكرونية الكروية تراوحت بين 105.2 و 1313.9 نانومتر (بمتوسط 356.09 نانومتر) وذلك باستخدام المجهر الإلكتروني الماسح عالي النفاذية FESEM. بينما كان متوسط الأبعاد المحسوب بطريقة التشتت الضوء الديناميكي DLS مساوياً لـِ 46.04 و 377 نانومتر. اختبار الفعالية المضادة للفطور في المختبر In vitro للجسيمات الناتجة تمت دراسته بواسطة طريقيتين مايكروبيولوجيتين هما الانتشار في الآبار وتمديد المرق الدقيق في كلتا الطريقتين كانت للجسيمات النّانوية تأثير أكبر من الجسيمات بالحجم الأكبرضد الفطور، وكان التركيز المثبط الأدنى الـ MIC لجسيمات أوكسيد المغنيزيوم النّانويّة مساوياً لـِ 1.5 ، 6.25 ملغ / مل لكل من المبيضات البيض والراشاشيات السوداء على التوالي.
صُنعت جسيمات أوكسيد المغنيزيوم النّانويّة والمايكرونية بواسطة صبار التين الشوكي وتم تقييم فعاليتها المضادة للفطور المسببة لأمراض الهامة عند البشر كمبيضات البيض والرشاشيات السّوداء.وتم توصيف الجسيمات الناتجة بـِ UV-Vis ، FTIR ، DLS ، EDX ، .fesemأظهر تحليل مطيافية الأشعة المرئية وفوق البنفسجية قمة عند 300 نانومتر، وبيّن اختبار الأشعة تحت الحمراء أن المستقلبات الحيوية في النباتتلعب دورًا مهمًا في إرجاع أيونات المعدن ثم نموها لتعطي في النهاية جسيماتٍ نانويّة، كما يبين التحليل قمة قريبة من 400 تعود للرابطة Mg-O-Mg.يؤكد تحليل الـ edx وجود جسيمات أوكسيد المغنيزيوم.تراوحت أبعاد جسيمات أوكسيد المغنيزيوم النّانويّة ذات الشكل الكروي بين 15.5 و78.01 نانومتر (بمتوسط 42.28 نانومتر)، أما أبعاد الجسيمات المايكرونية الكروية تراوحت بين 105.2 و 1313.9 نانومتر (بمتوسط 356.09 نانومتر) وذلك باستخدام المجهر الإلكتروني الماسح عالي النفاذية fesem.بينما كان متوسط الأبعاد المحسوب بطريقة التشتت الضوء الديناميكي dls مساوياً لـِ 46.04 و 377 نانومتر.اختبار الفعالية المضادة لفطور في المختبر 体外 لجسيمات الناتجة تمت دراسته بواسطة طريقيتين مايكروبيولوجيتين هما الانتشار في الآبار وتمديد المرق الدقيق في كلتاالطريقتين كانت لجسيمات النّانوية تأثير أكبر من الجسيمات بالحجم الأكبرضد الفطور، وكان التركيز المثبط الأدنى ال micـ لجسيمات أوكسيد المغنيزيوم النّانويّة مساوياً لـِ 1.5 ، 6.25 ملغ / مل لكل من المبيضات البيض والراشاشيات السوداء على التوالي.
{"title":"الاصْطنَاع الخارج خلوي لجسيمات أوكسيد المغنيزيوم في المستوى النّانويّ والمايكروني باستخدام نبات الصّبار كمعمل حيويّ: وفعاليّتها المضادة لفطور المبيضات البيض والرشاشية السّوداء","authors":"Taif AlHoly, Walid Khaddam","doi":"10.35516/jjps.v16i4.864","DOIUrl":"https://doi.org/10.35516/jjps.v16i4.864","url":null,"abstract":"صُنعت جسيمات أوكسيد المغنيزيوم النّانويّة والمايكرونية بواسطة صبار التين الشوكي وتم تقييم فعاليتها المضادة للفطور المسببة للأمراض الهامة عند البشر كمبيضات البيض والرشاشيات السّوداء. وتم توصيف الجسيمات الناتجة بـِ UV–Vis، FTIR، DLS، EDX، .FESEMأظهر تحليل مطيافية الأشعة المرئية وفوق البنفسجية قمة عند 300 نانومتر، وبيّن اختبار الأشعة تحت الحمراء أن المستقلبات الحيوية في النبات تلعب دورًا مهمًا في إرجاع أيونات المعدن ثم نموها لتعطي في النهاية جسيماتٍ نانويّة، كما يبين التحليل قمة قريبة من 400 تعود للرابطة Mg-O-Mg. يؤكد تحليل الـ EDX وجود جسيمات أوكسيد المغنيزيوم. تراوحت أبعاد جسيمات أوكسيد المغنيزيوم النّانويّة ذات الشكل الكروي بين 15.5 و78.01 نانومتر (بمتوسط 42.28 نانومتر)، أما أبعاد الجسيمات المايكرونية الكروية تراوحت بين 105.2 و 1313.9 نانومتر (بمتوسط 356.09 نانومتر) وذلك باستخدام المجهر الإلكتروني الماسح عالي النفاذية FESEM. بينما كان متوسط الأبعاد المحسوب بطريقة التشتت الضوء الديناميكي DLS مساوياً لـِ 46.04 و 377 نانومتر. اختبار الفعالية المضادة للفطور في المختبر In vitro للجسيمات الناتجة تمت دراسته بواسطة طريقيتين مايكروبيولوجيتين هما الانتشار في الآبار وتمديد المرق الدقيق في كلتا الطريقتين كانت للجسيمات النّانوية تأثير أكبر من الجسيمات بالحجم الأكبرضد الفطور، وكان التركيز المثبط الأدنى الـ MIC لجسيمات أوكسيد المغنيزيوم النّانويّة مساوياً لـِ 1.5 ، 6.25 ملغ / مل لكل من المبيضات البيض والراشاشيات السوداء على التوالي.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139158135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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