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Fecal Matter as a Bio-indicator of Heavy Metal Toxification in Punjab Urial 粪便作为旁遮普乌拉尔地区重金属中毒的生物指标
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010285
Sumaira Mazhar, Adeeba Syed, B. N. Khan, Roheela Yasmin
Heavy metals are a major class of pollutants that are responsible for high level of toxicity in living beings. These metals have the tendency to bio-accumulate in the living tissues; their levels can be determined in the various organs of the body. Most of the old methods used for the indication of heavy metal contamination in the environment usually involve the killing of animals, whereas current study is used to determine the heavy metal contamination in fecal matter, feed, water and soil without causing any harm to the lives of animals. Observed level of heavy metal like cadmium (0.0073 ppm to 0.020 ppm), lead (0.029 ppm to 0.036 ppm), zinc (4.88 ppm to 5.326 ppm) and copper (0.118 ppm to 0.135 ppm) showed that their amounts are significantly high in fecal sample of Punjab Urial as compared to other samples collected both from Lahore zoo as well as Bahawalpur zoo.
重金属是一类对生物具有高度毒性的主要污染物。这些金属有在活组织中生物积累的倾向;它们的水平可以在身体的各个器官中确定。以往用于测定环境中重金属污染程度的方法大多采用宰杀动物的方法,而目前的研究则是在不损害动物生命的情况下测定粪便、饲料、水和土壤中的重金属污染程度。观察到的重金属水平,如镉(0.0073 ppm至0.020 ppm),铅(0.029 ppm至0.036 ppm),锌(4.88 ppm至5.326 ppm)和铜(0.118 ppm至0.135 ppm)表明,与从拉合尔动物园和巴哈瓦尔布尔动物园收集的其他样本相比,旁遮普乌里亚尔粪便样本中的重金属含量明显较高。
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引用次数: 0
Analysis of Human Beta Globin Gene and its Sequence Variants 人-珠蛋白基因及其序列变异分析
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010320
M. Shoaib, H. Mubeen, S. Raza
Hemoglobin is a protein commonly found in in red blood cells of all vertebrates and invertebrates. It consists of two chains, mainly alpha and beta chain. The globin genes are used as a model system to study gene expression in various eukaryotic organisms. Moreover, these genes controls the gene regulation process. Mutations in these genes can cause destruction in various regulatory pathways encoded by beta globin genes. The introns in beta globin genes mostly interrupts the sequence between codons 30-31 and between codons 104-105. The study involves a comparative genomics approach for identification and analysis of selected human beta globin gene (HBB) by using various computational tools. The nucleotide sequence of HBB gene was obtained from Genbank-NCBI and analyzed to study the transcripts within the coding region of gene by using ensemble software. These transcript maps will help to understand the regulatory features of genes, splice variants and putative indels in the reading frame. Also, the protein encoded by coding region of HBB gene was further analyzed to study various protein domains, profiles and signatures. This was done by using Interpro and CDD domain analysis software. The results are presented along with the proposed utilization of the studied gene. Comparative approaches are not only useful for evolutionary analysis but also can help to understand the function of various conserved genes.
血红蛋白是一种常见的蛋白质,存在于所有脊椎动物和无脊椎动物的红细胞中。它由两条链组成,主要是α链和β链。珠蛋白基因被用作研究真核生物基因表达的模型系统。此外,这些基因还控制着基因调控过程。这些基因的突变会破坏由-珠蛋白基因编码的各种调控途径。球蛋白基因中的内含子主要中断密码子30-31之间和密码子104-105之间的序列。本研究采用比较基因组学方法,利用各种计算工具对选定的人类-珠蛋白基因(HBB)进行鉴定和分析。从Genbank-NCBI中获取HBB基因的核苷酸序列,利用集成软件对基因编码区内的转录本进行分析研究。这些转录图谱将有助于理解基因的调控特征,剪接变异体和阅读框中假定的索引。进一步分析了HBB基因编码区所编码的蛋白质,研究了各种蛋白质结构域、谱和特征。利用Interpro和CDD域分析软件进行分析。研究结果与所研究基因的利用建议一起提出。比较方法不仅对进化分析有用,而且有助于了解各种保守基因的功能。
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引用次数: 0
Role of Nanoparticles to Improve Agricultural Practices and Plant Production 纳米粒子在改善农业实践和植物生产中的作用
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010317
S. M. Hassan, Farhat Parveen, Asif Ibrahim, S. K. Hassan, A. Majeed, N. Naeem
Nanotechnology is the outmost emerging technology of 21st century. It is an excellent approach to renovate the food and agricultural industry with the help of different modern strategies for the rapid disease detection, molecular management of diseases, improving the aptitude of plants to take up more nutrients. Nanotechnology has the ability to enhance yield of nutrient values and also plays an important role in improving developed systems to monitor ecological conditions. Moreover a number of nano-materials are well known for their potential plant growth promoting effects. This present review article highlights the potential effects of Nanoparticles on flowering, growth, and seed production in plants.
纳米技术是21世纪最重要的新兴技术。借助不同的现代策略,快速检测疾病,进行疾病的分子管理,提高植物吸收更多营养的能力,这是革新食品和农业工业的绝佳途径。纳米技术有能力提高营养价值的产量,而且在改善发达的生态条件监测系统方面也起着重要作用。此外,许多纳米材料以其潜在的植物生长促进作用而闻名。本文综述了纳米颗粒对植物开花、生长和制种的潜在影响。
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引用次数: 0
Comparative assessment of antifungal potential of Zingiber officinale and Allium sativum against standard antifungal drugs Amphotericin B and Nystatin 生姜和葱对标准抗真菌药物两性霉素B和制霉菌素抗真菌潜能的比较评价
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010180
W. Mehmood, Shoaib Ahmad Siddiqi, N. Naeem, Hira Minhas
ABSTRACT: This study was conducted to investigate theantifungal activity of garlic and ginger on a food derived fungus.Aqueous extracts of garlic (Allium sativum) and ginger (Zingiber officinale) were tested for their antifungal activity and their activities were compared with antifungal drugs nystatin and amphotericin B. The extracts were 40-50% serially diluted and different concentrations were obtained. Sterile strips were dipped in different concentrations of garlic and ginger. Strips were placed on petri plates containing fungus culture. After 24-48 hours incubation at 37 oC, antifungal zones of different diameter were obtained and measured in different plates. The results showed that garlic is more effective in its antifungal activity than ginger. The study demonstrated the potent activity of garlic against tested fungi which encourages its use as a suitable alternative drug for controlling fungal food spoilage. Since garlic is an herbal product with no known side-effects on human healthiest active ingredients may serve as safe food preservatives. This can ultimately increase the shelf life and also maintain the quality of preserved food. Purification and formulation of the garlic would give a true antifungal activity comparable to standard antifungal drugs., , antifungal activity,, 
摘要:本试验旨在研究大蒜和生姜对一种食源性真菌的抑菌作用。对大蒜(Allium sativum)和生姜(Zingiber officinale)水提物的抗真菌活性进行了测定,并与抗真菌药物制霉菌素(nystatin)和两性霉素b (amphotericin b)进行了比较。无菌试纸浸泡在不同浓度的大蒜和生姜中。将试纸条置于含有真菌培养物的培养皿上。37℃孵育24-48小时后,得到不同直径的抑菌带,并在不同的平板上测定。结果表明,大蒜的抗真菌活性优于生姜。该研究表明,大蒜对被测试真菌的有效活性,鼓励将其作为控制真菌食品腐败的合适替代药物。由于大蒜是一种草药产品,对人体健康没有已知的副作用,大多数活性成分可以作为安全的食品防腐剂。这最终可以延长保质期,并保持保存食品的质量。大蒜的纯化和配方将提供与标准抗真菌药物相当的真正抗真菌活性。,抗真菌活性,,
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引用次数: 1
Mediterranean Diet Pyramid: a Pivot Role in Dilemma of Anti-Aging 地中海饮食金字塔:在抗衰老困境中的支点作用
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010322
N. Naeem, Saima Ahmad, Shoaib Ahmad Siddiqi, Mona Hassan
Mediterranean diet reflects traditional dietary habits and foods of mediterrranean people, historically associated with good health.Mediterranean diet pyramid having the basis of dietary habits and food pattern more likely according to Crete, Greece and Italy, where life expectancy ratio among adults is greatest in the world. The diet is categorized by plentiful plant foods, fresh fruits as the usual daily dessert, olive oil as the major source of fat, dairy products particularly cheese and milk,fish and poultry in moderate to low amount, zero to four eggs consumed weekly with the consumption of red meats and wine in low amounts.All research studies included in this study emphasize on the fact that combinations of the foods in mediterrranean diet impart beneficial impact on the chronic disease prevention as well as their management primarily due to the nutraceutical nature of the ingredients rich in substances like omega 3 & 6, vitamins, minerals, polyphenols and functional fibers likely to be effective in cardiovascular disease risk factors and biomarkers of metabolic syndrome.
地中海饮食反映了地中海人的传统饮食习惯和食物,历史上与良好的健康联系在一起。以克里特岛、希腊和意大利的饮食习惯和饮食模式为基础的地中海饮食金字塔,这些国家的成年人预期寿命是世界上最高的。这种饮食包括丰富的植物性食物,新鲜水果作为日常甜点,橄榄油作为脂肪的主要来源,乳制品,尤其是奶酪和牛奶,适量食用鱼和家禽,每周食用0到4个鸡蛋,少量食用红肉和葡萄酒。本研究中包含的所有研究都强调这样一个事实,即地中海饮食中的食物组合对慢性疾病的预防和管理具有有益的影响,这主要是由于富含omega - 3和6、维生素、矿物质、多酚和功能性纤维等物质的营养性质,可能对心血管疾病的危险因素和代谢综合征的生物标志物有效。
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引用次数: 0
Response of Microbes Associated with Internal and External Surfaces of Blattella germanica towards Hydrophobicity and Autoaggregation 德国小蠊内外表面相关微生物对疏水性和自聚集的响应
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010283
A. Qurashi, U. Rafi, Muqaddas Niaz
German cockroaches (Blattella germanica) are distributed worldwide. Pathogens present on cockroach have always raised safety concerns. Present study aimed to check the bacterial autoaggregation and hydrophobicity potential. Cockroaches were trapped from house sewerage of Lahore, Pakistan in March 2016 and bacteria were isolated from external as well as internal part of cockroach. For isolation from internal colon part of digestive tract were serially diluted and selected (IM1 and IM3) from bacterial growth on agar plates while isolates from external surfaces of cockroach were selected by surface swabbing (EM4, EM5 and EM6). Morphological and biochemical characterization of isolates showed diverse morphology. The auto aggregation was recorded in all isolates after 4 hours of culture incubation. Results of hydrophobicity assay showed that isolates IM1, IM3, EM5 showed 98.09 %, 80.5 %, 90.33 % affinity towards toluene, respectively. Bacteria commonly associated with common pests, cockroaches showed the tendency to adhere to different biotic and abiotic surfaces. Isolate IM3 showed 35 % auto-aggregation after 4 hours while isolate EM4 showed 47 % aggregation as compared to rest of isolates. Result showed that on the basis of bacterial cell surface hydrophobicity and auto aggregation tendency of cells towards adherence and aggregation on host increases.
德国小蠊(Blattella germanica)分布于世界各地。蟑螂身上的病原体一直引起人们对安全的担忧。本研究旨在检测细菌的自聚集性和疏水性。2016年3月,在巴基斯坦拉合尔的住宅污水中捕获了蟑螂,并从蟑螂的外部和内部分离出细菌。采用琼脂平板上细菌生长的方法,从蟑螂的外表面(EM4、EM5和EM6)进行擦拭,并对大肠内消化道部分进行连续稀释和筛选(IM1和IM3)。菌株形态和生化特征表现出多样性。培养4小时后,所有分离株均出现自聚集现象。结果表明,分离株IM1、IM3、EM5对甲苯亲合力分别为98.09%、80.5%、90.33%。蟑螂通常与常见害虫有关,蟑螂表现出粘附在不同生物和非生物表面的倾向。分离物IM3在4小时后显示35%的自聚集,而分离物EM4与其他分离物相比显示47%的自聚集。结果表明,在细菌细胞表面疏水性和自聚集的基础上,细胞对宿主的粘附和聚集倾向增加。
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引用次数: 0
Statistical Aspects of Thalassemia Major: A Case Study in Punjab Pakistan 地中海贫血的统计方面:巴基斯坦旁遮普的案例研究
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010177
Fehmeeda Naz, M. Ismail, Shahid Pervaiz Rana
Thalassemia major is becoming one of the leading health problems in Pakistan. A case study on 204 patients was carried out at Fatimid Foundation Lahore. The objective of the current study was to investigate the prevalence of the disease in patients related to physical and socio-demographic aspects. The questionnaire was designed to collect the information on factors regarding the gender, age, weight, height, blood group, Hb level, ferritin level, age at the diagnosis, age at the first transfusion, complications associated with the disease and some information was collected from parents relating their education, awareness about disease and their relation. Descriptive and analytical analysis were performed by using numbers & percentages, Chi- Square, Phi and V values, Kendall’s Tau-B techniques. Descriptive analysis showed that disease is more common in males (61.3%) and risk factor blood group observed where (91.6%) patients had positive blood groups. Consanguinity was found to be a great risk factor. In bivariate analysis factors age group, caste, Splenectomy, enlarged liver, patients’ education, hepatitis C are found to be significantly associated with the gender and blood transfusion in a month, ferritin level, bones problem and hepatitis Care associated with age. It has observed from the results that thalassemia is an inherited blood disease and can be prevented by creating awareness in people of our country.
严重地中海贫血正在成为巴基斯坦的主要健康问题之一。在拉合尔法蒂玛基金会对204名患者进行了个案研究。本研究的目的是调查与身体和社会人口方面有关的患者中该病的流行情况。问卷收集了性别、年龄、体重、身高、血型、Hb水平、铁蛋白水平、诊断年龄、首次输血年龄、与疾病相关的并发症等因素的信息,并从父母那里收集了一些有关其教育程度、疾病意识及其关系的信息。描述性和分析性分析采用数字和百分比、卡方、Phi和V值、Kendall的Tau-B技术进行。描述性分析显示,男性多见(61.3%),观察到危险因素血型,其中91.6%的患者为阳性血型。血缘关系被发现是一个很大的危险因素。在双因素分析中发现,年龄、种姓、脾切除术、肝肿大、患者受教育程度、丙型肝炎与性别、一个月输血次数、铁蛋白水平、骨骼问题和肝炎护理与年龄相关。它从结果中观察到,地中海贫血是一种遗传性血液疾病,可以通过提高我国人民的认识来预防。
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引用次数: 0
Morpho-Physiological Variations in Pearl Millet [Pennisetum glaucum L. (R.Br)] in Response to Foliar Applications of NPK 珍珠粟[Pennisetum glaucum L. (R.Br)]形态生理变化对氮磷钾叶面施用的响应
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010282
K. Hussain, K. Nawaz, A. Majeed, E. H. Siddiqi
This study was carried out to evaluate the response of Pearl millet to foliar applications of NPK fertilizer with different levels i.e. T (control), T (NPK 4:4:3), T (NPK 8:8:6). Foliar applications of NPK fertilizer enhanced the growth and chlorophyll 0 1 2 contents of pearl millet. Maximum increase in growth attributes were recorded in T (NPK 8:8:6) while, high increase in chlorophyll 2 and carotenoides contents of pearl millet was measured in T (NPK 4:4:3) as compared to T0 (control) and T (NPK 8:8:6) at both 1 2 + seedling and vegetative stages. Variations were also observed in chemical analysis for N and K at both stages of growth under NPK applications. It was concluded that NPK are the major nutrients required for plant growth but its applications are more effective than applications in root medium.
本试验旨在评价珍珠粟对不同氮磷钾水平(T(对照)、T (NPK 4:4:3)、T (NPK 8:8:6)叶面施肥的响应。叶面施氮磷钾促进了珍珠粟的生长和叶绿素0 - 12含量。在12 +苗期和营养期,与对照和T (NPK 8:8:6)相比,T (NPK 4:4:3)处理珍珠谷子的叶绿素2和类胡萝卜素含量均有较高的提高。氮磷钾施用对两个生长阶段氮素和钾素的化学分析也有影响。综上所述,氮磷钾是植物生长所需的主要养分,但施用氮磷钾比根基质施用更有效。
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引用次数: 0
Crimean-Congo Hemorrhagic Fever--Distribution, Diagnosis, Treatment and Control Measures 克里米亚-刚果出血热的分布、诊断、治疗和控制措施
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010319
SamrahTahir Khan, H. Hashim, Wajeeha Hamid, S. Mehmood, F. Qamar
The Crimean Congo Hemorrhagic Fever (CCHF), is a highly pathogenic disease that contributes to high morbidity and mortality. It is due to a tick-borne virus, capable of causing hemorrhagic fever that leads to death, specially affecting the human beings, therefore is of outmost importance in the current decade. The disease is widely distributed covering many continents with 40-60% of mortality. Although it is lethal, but still can be avoided; at both community as well as nosocomial level, by adopting proper strategy for management of the disease. This review is a comprehensive discussion about the Crimean Congo Hemorrhagic fever and covers the preventive and curative measures that can be adopted to prevent the outbreak of CCHF.
克里米亚刚果出血热(CCHF)是一种高致病性疾病,导致高发病率和死亡率。这是由于一种蜱传播的病毒,能够引起导致死亡的出血热,特别影响人类,因此是当前十年中最重要的。该病广泛分布于许多大洲,占死亡率的40-60%。虽然是致命的,但还是可以避免的;在社区和医院两级,通过采取适当的策略来管理疾病。这篇综述是对克里米亚刚果出血热的全面讨论,涵盖了预防克里米亚刚果出血热爆发可采取的预防和治疗措施。
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引用次数: 0
Evaluation of Water Quality of Nearby Village During Process of Composting at Industrial Scal 工业规模堆肥过程中村附近水质评价
Pub Date : 2020-05-05 DOI: 10.54692/lgujls.2017.010174
A. Ameen, J. Ahmad, S. Raza
The Lahore Compost Private Limited operates a composting plant. This company utilizes tons of organic waste to make a conditioner and organic fertilizer. The organic waste is transported to the Mehmood booti , landfill site ring road Lahore. This study was conducted to evaluate the parameters that effect the water quality of nearby village due to process of composting takes place in composting plant. The results from this study concluded that composting plant do not affect the water quality and all physical, chemical parameters are according to the WHO standards.
拉合尔堆肥私人有限公司经营一家堆肥厂。这家公司利用成吨的有机废物制造调理剂和有机肥料。这些有机废物被运送到拉合尔环城路的马哈茂德布提垃圾填埋场。本研究旨在评估堆肥厂进行堆肥过程对附近村庄水质的影响参数。研究结果表明,堆肥厂对水质无影响,各项理化指标均符合世界卫生组织标准。
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引用次数: 0
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Lahore Garrison University Journal of Life Sciences
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