Pub Date : 2020-08-12DOI: 10.54692/lgujls.2020.0403110
S. Khalid, N. Aslam, S. Bashir, H. Waseem
ABSTRACT:Almost 12% of the world's population is getting renal stones at some stage in their lifetime. Improper diet, high body weight and excessive use of certain supplements are the main reasons to enhance renal issues. The severity of the disease depends on the types, location and age of the person. Present study was conducted to find out the determinants of renal stones among the adults age (18-50 years) attending the Urology Department of Sir Ganga Ram Hospital, and Mayo Hospital, Lahore. For this, 100 patients suffering from renal stones were selected randomly; relevant data were collected through pre-tested questionnaire, SPSS version 21.0 was used for data analysis. Results showed that the prevalence of calcium oxalate stone was 40%, uric acid stones 34%, struvite stones 15% and unknown stones were 11%. Renal stones were more prevalent among 18 to 30-year-old patients in males (54%) as compared to females (46%). Overweight, dietary habits, dehydration, cigarette smoking and sedentary life style, legume consumption, green leafy vegetables and tomatoes without peeling were considered as major risk factors for the prevalence of renal stones.
摘要:全球约12%的人口在一生中的某个阶段会患上肾结石。饮食不当、体重过高和过量使用某些补品是加重肾脏问题的主要原因。这种疾病的严重程度取决于患者的类型、位置和年龄。本研究旨在找出在拉合尔Sir Ganga Ram医院和Mayo医院泌尿科就诊的18-50岁成人肾结石的决定因素。为此，随机选取100例肾结石患者;通过预测问卷收集相关数据，使用SPSS 21.0版本进行数据分析。结果草酸钙结石占40%，尿酸结石占34%，鸟粪石结石占15%，未知结石占11%。肾结石在18 - 30岁男性患者中(54%)比女性(46%)更为普遍。超重、饮食习惯、脱水、吸烟和久坐不动的生活方式、食用豆类、绿叶蔬菜和不去皮的西红柿被认为是肾结石患病率的主要危险因素。
{"title":"Risk Factors of Renal Stones among Adult Patients Visiting Tertiary Care Hospitals, Lahore","authors":"S. Khalid, N. Aslam, S. Bashir, H. Waseem","doi":"10.54692/lgujls.2020.0403110","DOIUrl":"https://doi.org/10.54692/lgujls.2020.0403110","url":null,"abstract":"ABSTRACT:Almost 12% of the world's population is getting renal stones at some stage in their lifetime. Improper diet, high body weight and excessive use of certain supplements are the main reasons to enhance renal issues. The severity of the disease depends on the types, location and age of the person. Present study was conducted to find out the determinants of renal stones among the adults age (18-50 years) attending the Urology Department of Sir Ganga Ram Hospital, and Mayo Hospital, Lahore. For this, 100 patients suffering from renal stones were selected randomly; relevant data were collected through pre-tested questionnaire, SPSS version 21.0 was used for data analysis. Results showed that the prevalence of calcium oxalate stone was 40%, uric acid stones 34%, struvite stones 15% and unknown stones were 11%. Renal stones were more prevalent among 18 to 30-year-old patients in males (54%) as compared to females (46%). Overweight, dietary habits, dehydration, cigarette smoking and sedentary life style, legume consumption, green leafy vegetables and tomatoes without peeling were considered as major risk factors for the prevalence of renal stones.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127500831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402101
Bakhtawar Javed Piracha, Muhammad Shafique, A. A. Shahid, Amna Sajid, Faqeeha Javed Faqeeha Javed, Abida Shehzadi Abida Shehzadi, Muhammad Shehzad
Forensic Science is robustly associated with DNA that has a complex genetic blue print information intended to resolve the queries related to civil cases, judiciary, investigatory purposes and crime scenes. The study was carried out to contribute the data set of RMYSTRs (Rapidly Mutating YSTRs) globally. The study focused on the significance of using RM-YSTRs as compared to the set of 9-17 YSTRs (mutation rate 1 × 10-2), as the later one is not being able to attain a high magnitude of male relatives differentiation. In the present work, 100 Sindh DNA male samples were collected, by genotyping the amplified samples, using RM-YSTRs, having a mutation rate of 1 × 10-3. Forensic efficiency parameters such as, PIC (Polymorphism Information Content) was observed in the range of 0.7745 at locus DYS576 to 0.9313 at locus DYF399S1b, maximum gene diversity (0.9350) was found at locus DYF399S1b, minimum gene diversity (0.7978) was seen at locus DYS576. Highest value of allele frequency was found as 0.350 with the allele no 18 at locus DYS576. The results had shown that haplotype frequency and haplotype diversity value were 0.010 and 0.99297, as no haplotype was repeated, which highlighted on the fact that nearly complete male individualization can be obtainedusing RM YSTRs, thus helpful in avoiding the adventitious matches or exclusion or inclusion of male relatives in forensics cases work. It might be an initiative towards establishing RM YSTR database of Pakistani Sindhi population.
{"title":"Forensic Genetic Analysis of 11 Rapidly Mutating Y-STRs (RM-YSTRs) in Sindhi Population of Pakistan","authors":"Bakhtawar Javed Piracha, Muhammad Shafique, A. A. Shahid, Amna Sajid, Faqeeha Javed Faqeeha Javed, Abida Shehzadi Abida Shehzadi, Muhammad Shehzad","doi":"10.54692/lgujls.2019.0402101","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402101","url":null,"abstract":"Forensic Science is robustly associated with DNA that has a complex genetic blue print information intended to resolve the queries related to civil cases, judiciary, investigatory purposes and crime scenes. The study was carried out to contribute the data set of RMYSTRs (Rapidly Mutating YSTRs) globally. The study focused on the significance of using RM-YSTRs as compared to the set of 9-17 YSTRs (mutation rate 1 × 10-2), as the later one is not being able to attain a high magnitude of male relatives differentiation. In the present work, 100 Sindh DNA male samples were collected, by genotyping the amplified samples, using RM-YSTRs, having a mutation rate of 1 × 10-3. Forensic efficiency parameters such as, PIC (Polymorphism Information Content) was observed in the range of 0.7745 at locus DYS576 to 0.9313 at locus DYF399S1b, maximum gene diversity (0.9350) was found at locus DYF399S1b, minimum gene diversity (0.7978) was seen at locus DYS576. Highest value of allele frequency was found as 0.350 with the allele no 18 at locus DYS576. The results had shown that haplotype frequency and haplotype diversity value were 0.010 and 0.99297, as no haplotype was repeated, which highlighted on the fact that nearly complete male individualization can be obtainedusing RM YSTRs, thus helpful in avoiding the adventitious matches or exclusion or inclusion of male relatives in forensics cases work. It might be an initiative towards establishing RM YSTR database of Pakistani Sindhi population.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"293 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122794197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402109
M. A. Rana, Asmara Imtiaz, K. Ahmad, Iqra Shafiq
ABSTRACT:Most of the bacteria of Enterobacteriaceae family are pathogenic and cause urinary tract infections, Pneumonia and diarrheal diseases. It was noticed that antimicrobials resistance of bacteria is increasing day by day that brought expanded healthcare costs due to morbidity and mortality from treatment failures. In the present study three of gram negative bacteria Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis of Enterobacteriaceae family were considered. Antibiotic susceptibility of these bacteria was checked against clinical samples by Kirby Bauer test commonly known as disc diffusion method. Eleven commercially available antibiotics i.e. Fosmomycin (FOS), Imipenem (IMP), Tazobactum (TZP), Meropenem (MEM), Amikacin (AK), Tobramycin (TOB), Nnitrofurantoin (F), Doxycycline (DO), Norfloxacin (NOR), Gentamycin (CN), and Nalidixic Acid (NA) were used to check efficacy against selected microbes.Comparison of results produced by these bacteria showed variability in resistance pattern as FOS>IPM>MEM>AK>F>F>TZP>CN>TOB>DO>NOR>NA. Fosmomycin, Imipenem, Amikacin and Meropenem were found as most effective one among all selected antibiotics
{"title":"Antibiogram of Uropathogenic Bacterial Isolates from Urine Samples","authors":"M. A. Rana, Asmara Imtiaz, K. Ahmad, Iqra Shafiq","doi":"10.54692/lgujls.2019.0402109","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402109","url":null,"abstract":"ABSTRACT:Most of the bacteria of Enterobacteriaceae family are pathogenic and cause urinary tract infections, Pneumonia and diarrheal diseases. It was noticed that antimicrobials resistance of bacteria is increasing day by day that brought expanded healthcare costs due to morbidity and mortality from treatment failures. In the present study three of gram negative bacteria Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis of Enterobacteriaceae family were considered. Antibiotic susceptibility of these bacteria was checked against clinical samples by Kirby Bauer test commonly known as disc diffusion method. Eleven commercially available antibiotics i.e. Fosmomycin (FOS), Imipenem (IMP), Tazobactum (TZP), Meropenem (MEM), Amikacin (AK), Tobramycin (TOB), Nnitrofurantoin (F), Doxycycline (DO), Norfloxacin (NOR), Gentamycin (CN), and Nalidixic Acid (NA) were used to check efficacy against selected microbes.Comparison of results produced by these bacteria showed variability in resistance pattern as FOS>IPM>MEM>AK>F>F>TZP>CN>TOB>DO>NOR>NA. Fosmomycin, Imipenem, Amikacin and Meropenem were found as most effective one among all selected antibiotics","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133275918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402108
H. Sadia
ABSTRACT:In Pakistan, the outbreak of Covid-19 was announced by the Ministry of Health on February 26, 2020 and a single covid-19 case in the city of Karachi was confirmed. The number of cases started to multiply rapidly in Pakistan, although a strict lockdown was observed from 15th March 2020 until May, 2020. The most prevalent number of cases were in Punjab, decreasing to Sindh, KPK, Balochistan, Gilgit Baltistan, Islamabad, Azad Jamu, and Kashmir in fewer numbers. Pakistan had more chances of threats as its border is near to China, but on the contrary to the statistics proposed by WHO, the number of cases started to reduce in Pakistan from August 2020. This may be due to the hot climatic conditions of the country. According to CDC and WHO, 16th August, 2020, in Pakistan, out of 288041 confirmed cases, 6162 deaths occurred and 265624 cases have been recovered, which is a positive indication towards the recovery of other patients too. WHO has praised the strategies adopted by Pakistan to cope with this novel disease. However, it is also necessary to keep on maintaining the SOPs suggested by WHO in advance if the infection reappears.
{"title":"An Update on the Spread of COVID-19 in Pakistan","authors":"H. Sadia","doi":"10.54692/lgujls.2019.0402108","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402108","url":null,"abstract":"ABSTRACT:In Pakistan, the outbreak of Covid-19 was announced by the Ministry of Health on February 26, 2020 and a single covid-19 case in the city of Karachi was confirmed. The number of cases started to multiply rapidly in Pakistan, although a strict lockdown was observed from 15th March 2020 until May, 2020. The most prevalent number of cases were in Punjab, decreasing to Sindh, KPK, Balochistan, Gilgit Baltistan, Islamabad, Azad Jamu, and Kashmir in fewer numbers. Pakistan had more chances of threats as its border is near to China, but on the contrary to the statistics proposed by WHO, the number of cases started to reduce in Pakistan from August 2020. This may be due to the hot climatic conditions of the country. According to CDC and WHO, 16th August, 2020, in Pakistan, out of 288041 confirmed cases, 6162 deaths occurred and 265624 cases have been recovered, which is a positive indication towards the recovery of other patients too. WHO has praised the strategies adopted by Pakistan to cope with this novel disease. However, it is also necessary to keep on maintaining the SOPs suggested by WHO in advance if the infection reappears.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117217450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402105
Minnie Malik, M. Azam, Muhammadasim Raza Basra
ABSTRACT:Free radicals are the unstable electron-deficient species that reacts with different molecules to gain stability and to eliminate their unpaired condition. Antioxidant molecules neutralizes the free radicals by donating their electrons and inhibits the unwanted oxidative reactions in biological system. The imbalance between antioxidants and free radicals generated oxidative stress which leads to severe impairment of the biological systems. The purpose of the present review is to highlight the beneficial role of naturally occurring antioxidant systems in minimizing the damage and maintaining the homeostasis in the biological system. Enzymatic and non-enzymatic antioxidants are the major classes of natural antioxidants which executes diverse functions in the biological system to provide defense against the destructive accumulating effects of ROS/RNS. Superoxide dismutase (SOD) are responsible for providing first line of defense to thebiological system, by converting the superoxide anion radical (O oˉ) into hydrogen 2 peroxide (H O ) which eventually converted into water and oxygen. Non-enzymatic 2 2 antioxidants either endogenous or exogenous provides numerous crucial mechanisms to quench the ROS/RNS in the biological system. Endogenous antioxidants inhibits lipid peroxidation in the cellular membranes while the exogenous antioxidants acts as chain breakers and terminates the oxidative chain reaction. It is significant to sustain the level of exogenous antioxidants in the body through diet so that the normal biological processes can be maintained at optimal levels.
摘要:自由基是一种不稳定的缺电子物质，它与不同的分子发生反应以获得稳定性并消除它们的不配对状态。抗氧化分子通过提供电子来中和自由基，抑制生物系统中不需要的氧化反应。抗氧化剂和自由基之间的不平衡产生氧化应激，导致生物系统的严重损害。本综述的目的是强调天然存在的抗氧化系统在减少损害和维持生物系统内平衡方面的有益作用。酶促抗氧化剂和非酶促抗氧化剂是天然抗氧化剂的主要类别，它们在生物系统中发挥不同的功能，以防御ROS/RNS的破坏性积累效应。超氧化物歧化酶(SOD)负责为生物系统提供第一道防线，将超氧化物阴离子自由基(O O - H)转化为过氧化氢(H O)，最终转化为水和氧气。内源性或外源性非酶22抗氧化剂提供了许多重要的机制来抑制生物系统中的ROS/RNS。内源性抗氧化剂抑制细胞膜脂质过氧化，外源性抗氧化剂作为断链剂终止氧化链反应。通过饮食维持体内外源性抗氧化剂的水平，使正常的生物过程维持在最佳水平，具有重要意义。
{"title":"Impact of Natural Antioxidants on Biological Systems","authors":"Minnie Malik, M. Azam, Muhammadasim Raza Basra","doi":"10.54692/lgujls.2019.0402105","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402105","url":null,"abstract":"ABSTRACT:Free radicals are the unstable electron-deficient species that reacts with different molecules to gain stability and to eliminate their unpaired condition. Antioxidant molecules neutralizes the free radicals by donating their electrons and inhibits the unwanted oxidative reactions in biological system. The imbalance between antioxidants and free radicals generated oxidative stress which leads to severe impairment of the biological systems. The purpose of the present review is to highlight the beneficial role of naturally occurring antioxidant systems in minimizing the damage and maintaining the homeostasis in the biological system. Enzymatic and non-enzymatic antioxidants are the major classes of natural antioxidants which executes diverse functions in the biological system to provide defense against the destructive accumulating effects of ROS/RNS. Superoxide dismutase (SOD) are responsible for providing first line of defense to thebiological system, by converting the superoxide anion radical (O oˉ) into hydrogen 2 peroxide (H O ) which eventually converted into water and oxygen. Non-enzymatic 2 2 antioxidants either endogenous or exogenous provides numerous crucial mechanisms to quench the ROS/RNS in the biological system. Endogenous antioxidants inhibits lipid peroxidation in the cellular membranes while the exogenous antioxidants acts as chain breakers and terminates the oxidative chain reaction. It is significant to sustain the level of exogenous antioxidants in the body through diet so that the normal biological processes can be maintained at optimal levels.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122413550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402103
Khurram Shahzad, Iqra Karim, Syed Mustansar Abbass, Hira Karim Hira Karim, S. Hussain, Muhammad Ashfaq, Mehrine Rehman
ABSTRACT:In the present study, the degradation of organic dyes was performed in the presence of pure and nickel doped copper oxide nanoparticles. In this co-precipitation method was used for the synthesis of pure and nickel doped copper oxide nanoparticles. Sodium hydroxide was used as a precipitating agent. These nanoparticles were characterized by scanning electron microscopy (SEM), diffuse reflectance microscopy (DRS), and Ultraviolet-Visible spectroscopy. SEM results showed that the prepared particles were uniform, regular, pure, and in nano-range. DRS result showed that the copper oxide nanoparticles had a bandgap of 3.37 eV while nickel oxide had a bandgap of 2.29 eV. After doping of Ni the bandgap of copper oxide decreases from 3.37 eV to 2.39 eV. UVVisspectroscopic analysis showed that the photocatalytic degradation of organic dyes increases with time. Nickel doped copper oxide nanoparticles have excellentphotocatalytic degradation ability.
{"title":"Synthesis, Characterization, and Photocatalytic Degradation of Nickel Doped Copper Oxide Nanoparticles","authors":"Khurram Shahzad, Iqra Karim, Syed Mustansar Abbass, Hira Karim Hira Karim, S. Hussain, Muhammad Ashfaq, Mehrine Rehman","doi":"10.54692/lgujls.2019.0402103","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402103","url":null,"abstract":"ABSTRACT:In the present study, the degradation of organic dyes was performed in the presence of pure and nickel doped copper oxide nanoparticles. In this co-precipitation method was used for the synthesis of pure and nickel doped copper oxide nanoparticles. Sodium hydroxide was used as a precipitating agent. These nanoparticles were characterized by scanning electron microscopy (SEM), diffuse reflectance microscopy (DRS), and Ultraviolet-Visible spectroscopy. SEM results showed that the prepared particles were uniform, regular, pure, and in nano-range. DRS result showed that the copper oxide nanoparticles had a bandgap of 3.37 eV while nickel oxide had a bandgap of 2.29 eV. After doping of Ni the bandgap of copper oxide decreases from 3.37 eV to 2.39 eV. UVVisspectroscopic analysis showed that the photocatalytic degradation of organic dyes increases with time. Nickel doped copper oxide nanoparticles have excellentphotocatalytic degradation ability.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121773105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402107
S. Razi, S. Hasnain, Byung-Gee-Kim Byung-Gee-Kim
ABSTRACT:A chromium resistant Synechocystis species, AHZ-HB-MK (DQ 381960) was used in this study. It was aimed to characterize this strain at molecular level andto produce certain mutants with better chromate reduction abilities. The strain was irradiated with a 60Co source in a dose range of 0.5, 1, 2, 5, 10 and 20 Gy atdifferent stages of growth (after 5, 15 and 30 days) of incubation. The selected derivatives were characterized morphologically, physiologically and biochemically. Chromium reduction activity and the amount of Cr (VI) accumulated within the cells of all of the selected derivatives were estimated. Protein profiling was done to study the impact of gamma rays on the proteins. Protein profiling proved that in the case of cultures irradiated after 5 days of incubation, maximum protection of proteins to radiation damage was observed at 0.5, 1, 2 and 5 Gy. Maximum expression of stress proteins was observed in the derivative obtained at 2 Gy. This derivative was labeled as Synechocystis MKTR. Identification of these proteins was done by mass-spectrometry. Seventy-four peptides were identified from ten selected protein bands. In these peptides sll0170 a heat shock protein, slr2076 a 60kD chaperonin and sll0416 identified as 60kD chaperonin 2 and GroEL2. Whereas sll0170 was also a heat shock protein 70, slr1198 was identified to be an antioxidant protein and slr1516 was annotated as superoxide dismutase which may be involved in the chromate resistance in this strain.
{"title":"Proteomic Analysis of Cr (VI) Reducing Synechocystis sp. AHZ-HB-MK and its Mutant Obtained by Gamma Irradiation","authors":"S. Razi, S. Hasnain, Byung-Gee-Kim Byung-Gee-Kim","doi":"10.54692/lgujls.2019.0402107","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402107","url":null,"abstract":"ABSTRACT:A chromium resistant Synechocystis species, AHZ-HB-MK (DQ 381960) was used in this study. It was aimed to characterize this strain at molecular level andto produce certain mutants with better chromate reduction abilities. The strain was irradiated with a 60Co source in a dose range of 0.5, 1, 2, 5, 10 and 20 Gy atdifferent stages of growth (after 5, 15 and 30 days) of incubation. The selected derivatives were characterized morphologically, physiologically and biochemically. Chromium reduction activity and the amount of Cr (VI) accumulated within the cells of all of the selected derivatives were estimated. Protein profiling was done to study the impact of gamma rays on the proteins. Protein profiling proved that in the case of cultures irradiated after 5 days of incubation, maximum protection of proteins to radiation damage was observed at 0.5, 1, 2 and 5 Gy. Maximum expression of stress proteins was observed in the derivative obtained at 2 Gy. This derivative was labeled as Synechocystis MKTR. Identification of these proteins was done by mass-spectrometry. Seventy-four peptides were identified from ten selected protein bands. In these peptides sll0170 a heat shock protein, slr2076 a 60kD chaperonin and sll0416 identified as 60kD chaperonin 2 and GroEL2. Whereas sll0170 was also a heat shock protein 70, slr1198 was identified to be an antioxidant protein and slr1516 was annotated as superoxide dismutase which may be involved in the chromate resistance in this strain.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122834401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402102
Ambreen Ahmed, Saboor-ul-Hassan Saboor-ul-Hassan
ABSTRACT:The basic aim of this work is the isolation of mesophilic rhizobacteria from Gilgit- Baltistan region of Pakistan to study their impact on growth promotion of plants. Plant growth promoting rhizobacteria (PGPR) play their part in enhancing overall plant growth and can serve as potential bio-fertilizers. These isolated bacterial strains were screened by checking out their capability to produce auxin. Morphological and physiological characterization was also done. Plant growth-stimulating activities of these isolates were assessed through biological assay. Triticum aestivum seeds were inoculated with these isolates and the effect of these isolates was studied on the growth of wheat. Auxin producing ten bacterial strains were selected and used for the current study. Bacterially treated plants have shown significant enhancement in growth parameters as compared to non-treated plants. All bacterial strains have shown variable behavior. However, bacterial strain Mi5 performed most efficiently. In this case increment of 12.09, 68.82 and 39.89 % have been recorded in plant shoot, root and fresh weight as compared to control. Improvement in various plant growth parameters have shown that these indigenous bacterial strains can be used as biofertilizers in areas like with low temperature.
{"title":"Isolation and Characterization of Mesophilic Rhizobacteria from Gilgit-Baltistan and their Impact on the Growth of Triticum aestivum L.","authors":"Ambreen Ahmed, Saboor-ul-Hassan Saboor-ul-Hassan","doi":"10.54692/lgujls.2019.0402102","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402102","url":null,"abstract":"ABSTRACT:The basic aim of this work is the isolation of mesophilic rhizobacteria from Gilgit- Baltistan region of Pakistan to study their impact on growth promotion of plants. Plant growth promoting rhizobacteria (PGPR) play their part in enhancing overall plant growth and can serve as potential bio-fertilizers. These isolated bacterial strains were screened by checking out their capability to produce auxin. Morphological and physiological characterization was also done. Plant growth-stimulating activities of these isolates were assessed through biological assay. Triticum aestivum seeds were inoculated with these isolates and the effect of these isolates was studied on the growth of wheat. Auxin producing ten bacterial strains were selected and used for the current study. Bacterially treated plants have shown significant enhancement in growth parameters as compared to non-treated plants. All bacterial strains have shown variable behavior. However, bacterial strain Mi5 performed most efficiently. In this case increment of 12.09, 68.82 and 39.89 % have been recorded in plant shoot, root and fresh weight as compared to control. Improvement in various plant growth parameters have shown that these indigenous bacterial strains can be used as biofertilizers in areas like with low temperature.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"173 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123950852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-15DOI: 10.54692/lgujls.2019.0402106
S. Iqbal, Urooj Akhtar, Shoaib Siddique
ABSTRACT:Synthetic medicines are being replaced by those derived from natural resources because of cost burden and side effects of synthetic medicines. Zingiber officinale (Ginger) has been act as home remedy against the microbial infections. Its fresh juice has radio protective action that could be helpful in avoiding gamma radiation which exert harmful effects during the period of cancer treatment. It exhibited chemo defensive and antineoplastic action as well as has been useful to cure the inflammation in the body. Present study investigated in vitro antifungal efficacy against Aspergillus niger, Aspergillus oryzaea, Fusarium oxysporum and Fusarium tricinctum using disc diffusion technique. Maximum inhibitory zone against Aspergillus niger produced after 96 hours i.e. 46.66 ±2.5mm and minimum after 48 hours i.e. 41.66 ± 2.5mm. Maximum inhibitory zone against Aspergillus oryzaea was produced after 96 hours i.e. 23.33±1.73 mm and minimum at 48 hours i.e. 17.66 ± 1.73 mm. Maximum inhibitory zone against Fusarium oxysporum was produced after 96 hours i.e. 24.00 ± 1.5mm and minimum at 48 hours i.e. 18.00 ±1.5mm. Maximum inhibitory zone against Fusarium tricinctum was produced after 96 hours i.e. 38.33 ± 2.00 mm and minimum at 48 hours i.e. 35.00 ± 2.00 mm. These concentrates may have been proved to be effective antimicrobials and can be exploited in controlling superfluous microbial growth.
{"title":"In-Vitro Antifungal Efficacy of Zingiber Officinale","authors":"S. Iqbal, Urooj Akhtar, Shoaib Siddique","doi":"10.54692/lgujls.2019.0402106","DOIUrl":"https://doi.org/10.54692/lgujls.2019.0402106","url":null,"abstract":"ABSTRACT:Synthetic medicines are being replaced by those derived from natural resources because of cost burden and side effects of synthetic medicines. Zingiber officinale (Ginger) has been act as home remedy against the microbial infections. Its fresh juice has radio protective action that could be helpful in avoiding gamma radiation which exert harmful effects during the period of cancer treatment. It exhibited chemo defensive and antineoplastic action as well as has been useful to cure the inflammation in the body. Present study investigated in vitro antifungal efficacy against Aspergillus niger, Aspergillus oryzaea, Fusarium oxysporum and Fusarium tricinctum using disc diffusion technique. Maximum inhibitory zone against Aspergillus niger produced after 96 hours i.e. 46.66 ±2.5mm and minimum after 48 hours i.e. 41.66 ± 2.5mm. Maximum inhibitory zone against Aspergillus oryzaea was produced after 96 hours i.e. 23.33±1.73 mm and minimum at 48 hours i.e. 17.66 ± 1.73 mm. Maximum inhibitory zone against Fusarium oxysporum was produced after 96 hours i.e. 24.00 ± 1.5mm and minimum at 48 hours i.e. 18.00 ±1.5mm. Maximum inhibitory zone against Fusarium tricinctum was produced after 96 hours i.e. 38.33 ± 2.00 mm and minimum at 48 hours i.e. 35.00 ± 2.00 mm. These concentrates may have been proved to be effective antimicrobials and can be exploited in controlling superfluous microbial growth.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130144026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-06DOI: 10.54692/lgujls.2018.020147
M. Hanif, Samar Bashir
Swat valley is Himalayan moist temperate forest of Pakistan. It is also called mini Switzerland of Pakistan. This area has diverse flora. A wide diversity of mushrooms is present over here but number of species explored is very small. In the present study, a species of Amanita was collected during field tour and identified on the basis of morpho-anatomical and molecular markers which confirmed its identification as Amanita flavipes. BLAST analysis of this species has been re-investigated from new locality swat and is being reported first time from this area.
{"title":"e-investigation of Amanita flavipes: Identification and Characterization Based on rDNA-ITS Sequence, Morphology and Anatomy","authors":"M. Hanif, Samar Bashir","doi":"10.54692/lgujls.2018.020147","DOIUrl":"https://doi.org/10.54692/lgujls.2018.020147","url":null,"abstract":"Swat valley is Himalayan moist temperate forest of Pakistan. It is also called mini Switzerland of Pakistan. This area has diverse flora. A wide diversity of mushrooms is present over here but number of species explored is very small. In the present study, a species of Amanita was collected during field tour and identified on the basis of morpho-anatomical and molecular markers which confirmed its identification as Amanita flavipes. BLAST analysis of this species has been re-investigated from new locality swat and is being reported first time from this area.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127080278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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