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Antibacterial Activity of Alcoholic and Aqueous Extracts of Agaricus bisporus Against Food Borne Bacterial Pathogens 双孢蘑菇醇提液和水提液对食源性致病菌的抑菌活性研究
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.17
Alyaa Razooqi Hussein, E. Ali, Ebtehal Hamid
Two extracts of 50 mg.ml-1 Agaricus bisporus alcoholic and aqueous were used in order to study their effect against two pathogenic bacteria. Salmonella typhi and Staphylococcus aureus strains isolated from chicken and minced meat samples respectively, then identified according to biochemical tests. Antibacterial activity of Agaricus bisporus extracts (alcoholic and aqueous) was studied by agar well diffusion method. The results exhibited that aqueous mushroom extract has no effect against the two tested bacteria, while alcoholic extract of mushroom affect against the two tested bacteria and the effect against S. aureus was more than that against Salmonella typhi and the zone of inhibition was 15 mm and 9 mm respectively. The ethanol alcohol 96% and water were used as control. From this study, mushroom extracts especially ethanolic or alcoholic could be used to control the transmission of pathogenic bacteria from food
50毫克提取物两份。采用ml-1双孢蘑菇醇剂和水溶液对两种病原菌进行了抑菌效果研究。分别从鸡肉和肉糜样品中分离出伤寒沙门氏菌和金黄色葡萄球菌菌株,并根据生化试验进行鉴定。采用琼脂孔扩散法研究双孢蘑菇醇提液和水提液的抑菌活性。结果表明:香菇水提物对两种被试菌均无抑制作用,而香菇醇提物对两种被试菌及金黄色葡萄球菌的抑制作用均大于伤寒沙门氏菌,抑制区分别为15 mm和9 mm。以96%乙醇和水为对照。从这项研究中可以看出,蘑菇提取物,特别是乙醇或酒精提取物,可以用来控制食物中致病菌的传播
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引用次数: 1
Structural Characterization of NiO/Cr2O3Composites and Hydrothermal Synthesis, Properties Gas Sensing NiO/ cr2o3复合材料的结构表征及水热合成、性能及气敏
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.10
G. Mohammed, Tunis B.Hassan, Zainab T Abdulhamied
Pure Cr2O3 and NiO/Cr2O3 nanocomposite were luckily synthesized by simple hydrothermal technique. X-ray films diffraction (XRD), Filed Emission scanning electron microscopy (FESEM), Energy dispersive X-ray spectroscopy (EDS), were used to research the crystalline structures, surface morphologies and nanostructures, and element components and their valences of the as-synthesized samples. Moreover, gas sensors based on the synthesized pure Cr2O3 and NiO/ Cr2O3 composites were fabricated and their sensing behavior, to NH3.Gas sensing experiments reference that the NiO/ Cr2O3 composites showed much higher gas response and lower working temperature than those of pure Cr2O3. These results assure that the as-synthesized NiO/ Cr2O3 composites a favorable NH3 sensing material
采用简单水热法制备了纯Cr2O3和NiO/Cr2O3纳米复合材料。利用x射线薄膜衍射(XRD)、场发射扫描电子显微镜(FESEM)、能量色散x射线能谱(EDS)等技术对合成样品的晶体结构、表面形貌、纳米结构、元素组成及其价态进行了研究。此外,制备了基于合成的纯Cr2O3和NiO/ Cr2O3复合材料的气体传感器,并研究了它们对NH3的传感行为。气敏实验表明,与纯Cr2O3相比,NiO/ Cr2O3复合材料具有更高的气敏响应和更低的工作温度。这些结果保证了合成的NiO/ Cr2O3复合材料是一种良好的NH3传感材料
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引用次数: 1
Effect of NaCl and Sorbitol on the Production of Some Alkaloids of Fenugreek Cotyledons Derived Callus NaCl和山梨醇对胡芦巴子叶愈伤组织中部分生物碱生成的影响
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.14
S. A. Hassan, E. H. Jassim
Alkaloids often had pharmacological effects. The aim at this study is to increase some alkaloids in fenugreek. Callus induction occurred on cotyledon segments 5 mm long. Murshige and Skoog medium (MS) supplied with 1 mg.lˉ¹ of 2, 4 – D and 0.4 mg.lˉ¹ of kinetin was prepared. The best medium maintained callus was using MS medium supplied with 0.5 mg.lˉ¹ 2, 4-D and 0.5 mg.lˉ¹ kinetin. Callus formation was allowed to take place for a period of thirty days in the dark at 25°C±2. Calli were analyzed using high performance liquid chromatography (HPLC).Methanol callus extract showed high concentrations of some alkaloids compared to the methanol extract of cotyledons. For increasing, the concentration of secondary metabolites, NaCl was added at concentrations 0, 1, 1.5 or 2 g.lˉ¹, sorbitol at concentrations 0, 7, 8 or 9 g.lˉ¹. NaCl at 2 g.lˉ¹ led to significant increase in trigonelline reaching 1354.72 µg per 100 mg fresh weight of callus. NaCl at 1g.lˉ¹ increased choline and carpaine reached 400.82, 483.92 µg.mlˉ¹ per 100 mg respectively. Treatment with 9 g.lˉ¹ sorbitol increased trigonelline and carpaine reached 1666.41, 742.67 µg per 100 mg respectively, while treatment 8 g.lˉ¹ sorbitol significantly increased in choline recorded 588.87 µg per 100 mg.
生物碱通常具有药理作用。本研究旨在提高胡芦巴中某些生物碱的含量。愈伤组织发生在长5mm的子叶裂片上。Murshige和Skoog培养基(MS),含1mg。2, 4 - D和0.4 mg。制备了L - 1 - 1的激动素。维持愈伤组织的最佳培养基为添加0.5 mg的MS培养基。1 . 2, 4-D和0.5 mg。lˉ¹激动素。在25°C±2的黑暗条件下,让愈伤组织形成30天。采用高效液相色谱法对愈伤组织进行分析。与子叶甲醇提取物相比,甲醇愈伤组织提取物中某些生物碱含量较高。为了提高次生代谢物的浓度,分别在0、1、1.5或2 g.l - 1¹浓度下添加NaCl,在0、7、8或9 g.l - 1¹浓度下添加山梨醇。当NaCl浓度为2 g.l时,愈伤组织中葫芦巴碱含量显著增加,达到1354.72µg / 100 mg鲜重。1g NaCl。L - h + 1分别增加了400.82、483.92µg的胆碱和车碱。Ml - 1 / 100mg。9 g.l山梨醇处理使葫芦巴碱和车碱含量分别增加1666.41、742.67µg / 100 mg, 8 g.l山梨醇处理使胆碱含量显著增加588.87µg / 100 mg。
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引用次数: 4
Detection of luxS Gene in Serratia marcescens and rpoS Gene in Enterobacter cloacae Isolates Using PCR Reaction 粘质沙雷氏菌luxS基因和阴沟肠杆菌rpoS基因的PCR检测
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.18
N. A. Muhammed, M. B. Farhan, Z. Shabeeb
luxS and rpoS genes were involved in the regulation of some biological processes, which interfere with virulence of Serratia marcescens and Enterobacter cloacae bacterial species sequentially. luxS have an important activity in Quorum Sensing (QS) processes, but rpoS interfere with tolerance and resistance processes toward stress conditions correlating with its virulence. The study aimed to detect rpoS presence in E. cloacae and luxS gene presence in S. marcescens separately isolated from 16 leukaemia patients (4 of S. marcescens and 12 of E. cloacae), who characterised with bacteraemia by using primers designed for this purpose by using PCR technique. The results showed that all of the bacterial isolates of S. marcescens contain luxS gene, while no one of E. cloacae isolates show positive results for rpoS gene. The study concluded that S. marcescens has high virulence as a result of luxS presence, which controls Quorum sensing and prodigiosin formation and affecting host immunity. Moreover, the negative results of rpoS gene can be explained according to two possibilities: the gene can be found in the isolates with a copy differ from the registered sequences of the rpoS gene, or the gene did not present in those isolates with presence of alternatives offset its activities
luxS和rpoS基因参与调控一些生物过程,依次干扰粘质沙雷氏菌和阴沟肠杆菌的毒力。luxS在群体感应(Quorum Sensing, QS)过程中具有重要的活性,而rpoS则干扰与其毒力相关的胁迫条件的耐受性和抗性过程。本研究利用PCR技术设计的引物,对16例以菌血症为特征的白血病患者(粘质梭菌4例,阴沟梭菌12例)分离的黏质梭菌中rpoS基因和luxS基因进行检测。结果表明,所有粘质大肠杆菌分离株均含有luxS基因,而阴沟大肠杆菌分离株均未检测到rpoS基因。本研究认为,粘质葡萄球菌具有较高的毒力是由于luxS的存在,luxS控制群体感应和prodigisin的形成,并影响宿主免疫。此外,rpoS基因的阴性结果可以根据两种可能性来解释:该基因可以在与rpoS基因的注册序列不同的副本中发现,或者该基因不存在于存在替代其活性的分离株中
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引用次数: 0
Spectrophotometeric Determination of Ponceau 4R in the Several Beverages Using Mixed Micelles after Cloud Point Extraction 浊点萃取混合胶束分光光度法测定几种饮料中苯梭4R的含量
Pub Date : 2018-03-01 DOI: 10.22401/JUNS.21.1.02
M. Thani, S. A. Al-Ameri, M. Sultan
Ponceau 4R(E124) is azo dye that is mostly used in some of food products such as soft drinks, beverages, jelly and ham. A simple and sensitive procedure is established for the determination of ponceau 4R using cloud point extraction (CPE). In order to get the optimum conditions for ponceau 4R extraction, various analytical factors such as pH media, nonionic surfactant TX-114 or Brij-56, cationic surfactant CPC and equilibration temperature was investigated. The linearity of calibration graph was above the range of 0.05-1.7 µg ml-1. The LOD and LOQ using TX-114 were based to be 0.025, 0.075 µg ml-1 whereas for Brij-56 were found as 0.062, 0.187 µg ml-1 respectively. The proposed procedure was successfully employed for ponceau 4R detection in several beverages samples
Ponceau 4R(E124)是偶氮染料,主要用于软饮料、饮料、果冻、火腿等食品中。建立了一种简便、灵敏的云点提取法测定ponceau 4R的方法。考察了pH介质、非离子表面活性剂TX-114或Brij-56、阳离子表面活性剂CPC和平衡温度等因素对ponceau 4R提取的影响,确定了ponceau 4R的最佳提取条件。校准图线性范围在0.05 ~ 1.7µg ml-1以上。TX-114的LOD和LOQ分别为0.025、0.075µg ml-1,而Brij-56的LOD和LOQ分别为0.062、0.187µg ml-1。该方法成功地用于几种饮料样品的ponceau 4R检测
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引用次数: 8
Estimation of some Elements and Acids for the Different Grape Juice 不同葡萄汁中一些元素和酸的测定
Pub Date : 2018-03-01 DOI: 10.22401/JUNS.21.1.04
D. Jamil
This study included estimating the heavy elements of some types of grape juice of different origin and determining their proportions in juices and comparing them with the quality control of Iraq and observing whether it is suitable for human use or not. Calcium salts are observed as 137 ppm in C juice and 9.5 ppm of magnesium in juice B, for heavy metals the highest percentage of lead 0.19ppm recorded in juice A, 1.7 ppm of copper in B juice 3.5 ppm Iron in juice A, 0.8 ppm of zinc in B juice and A, 0.06 ppm of aluminum in B, 0.015 ppm of cobalt in juice A and B, 0.06 ppm of nickel in B juice, Sodium was 168.1 ppm in C juice, but the acids were found to have the highest rates were 3.1 ppm of malic acid in B, 156.7 ppm of acid in Juice of ascorbic B, 238.5 ppm of acid found in lemon juice 130ppm in juice B.
本研究包括对几种不同产地葡萄汁的重元素含量进行估算,确定其在葡萄汁中的比例,并与伊拉克的质量控制进行比较,观察其是否适合人类使用。钙盐是观察到的137 ppm C汁和9.5 ppm的镁汁B,对重金属铅的比例最高0.19 ppm记录在果汁,1.7 ppm的铜汁3.5 ppm铁汁,在B果汁和一个0.8 ppm的锌,铝的0.06 ppm B, 0.015 ppm的钴在A和B果汁,0.06 ppm的镍在B果汁,钠是168.1 ppm C汁,但酸被发现的最高利率3.1 ppm的苹果酸B,抗坏血药B汁中含有156.7 ppm的酸,柠檬汁中含有238.5 ppm的酸,B汁中含有130ppm的酸。
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引用次数: 0
Computational Method based Laplace Adomian Decomposition for Solving Delay Differential Equations of Fractional Order 基于拉普拉斯Adomian分解的分数阶时滞微分方程求解方法
Pub Date : 2018-03-01 DOI: 10.22401/jnus.21.1.20
Zaid Mohammed
In this paper we present a computational method for solving delay differential equations of fractional order by employing the Laplace Adomian decomposition method. This method is combined from the Laplace transforms and the Adomian decomposition method taking into account the Caputo derivative as a motivation to describe the fractional derivative. The method is a modification of the Adomian decomposition method and is tested on two examples in order to illustrate the pertinent feature of this method the results shows that the proposed method is an effective and powerful tool for solving delay differential equations of fractional order. A comparison with the exact solution and with the existing methods such as Adomian decomposition method and homotopy analysis method is made
本文给出了用拉普拉斯阿多米安分解方法求解分数阶时滞微分方程的一种计算方法。该方法结合了拉普拉斯变换和阿多米安分解方法,考虑了卡普托导数作为描述分数阶导数的动机。该方法是对Adomian分解方法的改进,并通过两个算例进行了验证,以说明该方法的相关特点,结果表明该方法是求解分数阶时滞微分方程的有效而有力的工具。并与精确解和现有的Adomian分解法、同伦分析法等方法进行了比较
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引用次数: 1
Sirtuin1, Inducible Nitric Oxide Synthase in Newly Diagnosed Iraqi Diabetic Patients 伊拉克新诊断糖尿病患者诱导型一氧化氮合酶Sirtuin1
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.01
Mahmood Abdulhameed Hazim, P. Saifullah, Beydaa Ahmed Abd
In Mammals Sirtuins (SIRTs) are members of the silent information regulator two family Sirtuin1 regulate a variety of cellular functions. It shows anti-oxidative, anti-inflammatory, and anti-apoptotic effects against cellular damage.SIRT1 conserves the cells by the mitochondrial biogenesis organizing, cellular energy and oxido reduction state. Vascular tissues are protected by SIRT1 too. In diabetic patients mutation in exon1 of SIRT1 (L107P) in insulin-generating cells conducted in over production of nitric oxide synthase (iNOS), TNF-α.Assessment of SIRT1 protein levels in newly diagnosed Iraqi diabetic in regards to family history Age, gender, and duration of disease, and its correlation with biochemical parameter in the same group. Measurement of iNOS, TNF-α.This study involved totally 40 volunteers. This group were subdivided (20) volunteers as newly diagnosis (DMT1) duration disease ˂1 year, group1 was subdivided to 10 male and 10 female: (10 with family history and 10 without family history) and group two healthy volunteers. Group1 All DMT1 were under insulin treatment. Healthy Volunteers (20) included in this study as control groups. None Healthy Volunteers were alcoholic, smoke, or having a history of (CVD) cardiovascular disease, thyroid disease, and hormonal abnormalities problems were exempted from this study.In the present study SIRT1, TNF-alpha, iNOS were measured by enzyme linked immune absorbent assay (ELISA). Biochemical parameters fasting glucose, body mass index (BMI), urea, creatinine and lipid profile that were measured by spectrophotometer technique in patients and healthy volunteers groups.The Statistical Analysis System- SAS (2012) program was used to effect of difference factors in study parameters. Least significant difference –LSD test (ANOVA) was used to significant compare between means. Estimate of correlation coefficient between difference parameters in this study.The results showed that a highly significant increase in levels of FBG, Urea, creatinine and lipid profile except HDL was decrease levels, BMI was decrease in newly diagnosis DMT1 with healthy volunteers groups. A significant decrease in SIRT1 protein, and a highly significant increase in TNF-alpha and iNOS in newly diagnosis DMT1 with healthy volunteers groups. A highly negative significantly correlation coefficient between SIRT1 and F.G., TG., VLDL,TG/HDL, A.I. and negative significantly correlation duration, urea, creatinine,T.CH., LDL. And non-significant correlation with age, BMI, HDL, CH/HDL and LDL/HDL.. Effect of gender were insignificant of SIRT1. Effect of family history in patients. SIRT1 were all insignificant in patients with family history as with non-family history group. SIRT1 could decreases in the sera of DMT1 patients with family history, the decreased SIRT1 level reflect a mutation in SIRT1 gene, the decreases in SIRT1 on increases the incident of DMT1 complication.
在哺乳动物中,Sirtuins (SIRTs)是沉默信息调控2家族的成员,Sirtuin1调节多种细胞功能。对细胞损伤具有抗氧化、抗炎、抗凋亡作用。SIRT1通过线粒体生物发生组织、细胞能量和氧化还原状态来保存细胞。血管组织也受到SIRT1的保护。糖尿病患者胰岛素生成细胞中SIRT1 (L107P)外显子1突变导致一氧化氮合酶(iNOS)、TNF-α过量产生。新诊断伊拉克糖尿病患者SIRT1蛋白水平与家族史、年龄、性别、病程的关系及其与同组生化指标的相关性iNOS, TNF-α的测定。这项研究共涉及40名志愿者。将20名志愿者分为新诊断(DMT1)病程小于1年,第1组分为男性10名,女性10名(有家族史10名,无家族史10名),第2组为健康志愿者。组1所有DMT1患者均接受胰岛素治疗。健康志愿者(20名)作为对照组。没有健康的志愿者是酒精,吸烟,或有(CVD)心血管疾病史,甲状腺疾病,激素异常问题被排除在本研究之外。本研究采用酶联免疫吸附试验(ELISA)检测SIRT1、tnf - α、iNOS。采用分光光度计技术测定患者组和健康志愿者组的空腹血糖、体重指数(BMI)、尿素、肌酐、血脂等生化指标。采用统计分析系统SAS(2012)程序对研究参数中不同因素的影响进行分析。采用最小显著性差异-LSD检验(ANOVA)进行均值间的显著性比较。估计本研究中不同参数之间的相关系数。结果显示,新诊断DMT1的健康志愿者组除HDL降低外,FBG、尿素、肌酐和血脂水平均显著升高,BMI降低。在新诊断为DMT1的健康志愿者组中,SIRT1蛋白显著降低,tnf - α和iNOS显著增加。SIRT1与fg、TG呈高度负相关。VLDL、TG/HDL、A.I.与持续时间、尿素、肌酐、T.CH呈显著负相关。低密度脂蛋白。与年龄、BMI、HDL、CH/HDL、LDL/HDL无显著相关。性别对SIRT1的影响不显著。家族史对患者的影响。有家族史组与无家族史组SIRT1均不显著。有家族病史的DMT1患者血清中SIRT1水平可降低,SIRT1水平的降低反映了SIRT1基因的突变,SIRT1水平的降低增加了DMT1并发症的发生率。
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引用次数: 0
Bacteriological Study for Detection The Role of Fructan Produced from Pseudomonas putida as Enhancer for Lactobacillus acidophilus Growth 恶臭假单胞菌产果聚糖对嗜酸乳杆菌生长促进作用的细菌学研究
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.15
A. R. Kazim
Fructan is a kind of biopolymer produced from plants, fungi, yeast and bacteria. It is classified as prebiotics food and enhanced the growth of probiotics bacterial strains which improves the health of the individual. This article aimed to study the production and extraction of fructan from Pseudomonas putida isolated from food samples and study the effect of fructan on the viable cell number of Lactobacillus acidophilus. The results showed that Pseudomonas putida have the ability to produce fructan when cultured on production medium (YPS), fructan exhibited as white powder when extracted from YPS and the amount of fructan was 3.6 mg/100 ml, also the results showed that fructan can increase the viable cell number of Lactobacillus acidophilus when added to MRS. FTIR analysis of fructan produced from Pseudomonas putida revealed that the functional groups found in chemical structure of fructan, which it was (C-O, C-H, O-H and C=O). [DOI: 10.22401/JUNS.21.1.15]
果聚糖是一种从植物、真菌、酵母和细菌中提取的生物聚合物。它被归类为益生元食品,促进益生菌菌株的生长,改善个体的健康状况。本文旨在研究从食品中分离的恶臭假单胞菌中提取果聚糖的工艺,并研究果聚糖对嗜酸乳杆菌活菌数的影响。结果表明,恶臭假单胞菌在生产培养基(YPS)上培养时具有产果聚糖的能力,从YPS中提取的果聚糖为白色粉末,果聚糖的量为3.6 mg/100 ml,加入mrs中,果聚糖可以增加嗜酸乳杆菌的活细胞数量,FTIR分析表明,由恶臭假单胞菌生产的果聚糖的化学结构中发现的官能团为(C-O);C- h, O- h和C=O)(DOI: 10.22401 / JUNS.21.1.15)
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引用次数: 1
Numerical Solution for Fractional Order Space-Time Burger's Equation Using Legendre Wavelet - Chebyshev Wavelet Spectral Collocation Method 用Legendre小波- Chebyshev小波谱配点法数值解分数阶时空Burger方程
Pub Date : 2018-03-01 DOI: 10.22401/JNUS.21.1.19
O. H. Mohammed, Mohammed G. S. AL-Safi, A. A. Yousif
In this article, a Legendre wavelet- Chebyshev wavelet spectral collocation method is proposed for solving fractional order space-time Burger's equation with the Legendre wavelet and Chebyshev wavelet operational matrices of fractional derivatives. The fractional derivative is described in the Caputo sense. The proposed method is based on Legendre wavelet-Chebyshev wavelet for space and time variables respectively. This method will reduc the problem under consideration to the solution of nonlinear algebraic equations. In order to confirm the efficiency of the proposed method, two numerical examples are implemented and comparing the numerical solution with the exact one, as well as, of other methods in given literatures, we demonstrate the high accuracy and efficiency of the proposed method.
本文利用分数阶导数的Legendre小波和Chebyshev小波运算矩阵,提出了求解分数阶时空Burger方程的Legendre小波- Chebyshev小波谱搭配方法。分数阶导数是用卡普托意义来描述的。该方法分别基于空间变量和时间变量的勒让德小波-切比雪夫小波。这种方法将所考虑的问题简化为非线性代数方程的解。为了验证所提方法的有效性,通过两个数值算例的算例,将所提方法的数值解与精确解以及文献中其他方法的数值解进行了比较,验证了所提方法的高精度和高效性。
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引用次数: 5
期刊
Journal of Al-Nahrain University-Science
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