AFB1-8,9-exo-epoxide (AFBO) is the highly toxic product of Aflatoxin B1 (AFB1). Glutathione S-transferases (GSTs) play pivotal roles in detoxifying AFB1 by catalyzing the conjugation of AFBO with glutathione (GSH). Although there are over 20 GST isozymes that have been identified in chicken, GST isozymes involved in the detoxification process of AFB1 have not been identified yet. The objective of this study was to determine which GST isozymes played key role in detoxification of AFB1. A total of 17 pcDNA3.1(+)-GST isozyme plasmids were constructed and the GST isozyme genes were overexpressed by 80–2,500,000 folds in the chicken Leghorn male hepatoma (LMH) cells. Compared to the AFB1 treatment, overexpression of GSTA2X, GSTA3, GSTT1L, GSTZ1-1, and GSTZ1-2 increased the cell viability by 6.5%–17.0% in LMH cells. Moreover, overexpression of five GST isozymes reduced the release of lactate dehydrogenase and reactive oxygen species by 8.8%–64.4%, and 57.2%–77.6%, respectively, as well as enhanced the production AFBO-GSH by 15.8%–19.6%, thus mitigating DNA damage induced by AFB1. After comprehensive evaluation of various indicators, GSTA2X displayed the best detoxification effects against AFB1. GSTA2X was expressed in Pichia pastoris X-33 and its enzymatic properties for catalyzing the conjugation of AFBO with GSH showed that the optimum temperature and pH were 20–25 °C and 7.6–8.6 as well as the enzymatic kinetic parameter Vmax was 0.23 nmol/min/mg and the Michaelis constant was 86.05 μmol/L with the AFB1 as substrate. In conclusion, GSTA2X, GSTA3, GSTT1L, GSTZ1-1, and GSTZ1-2 played key roles in AFB1 detoxification, which will provide new remediation strategies to prevent aflatoxicosis in chickens.
{"title":"Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin B1 in chicken liver","authors":"Jiang Deng, Zhe Peng, Zhiyuan Xia, Yixin Mo, Lijia Guo, Jintao Wei, Lvhui Sun, Meng Liu","doi":"10.1186/s40104-025-01189-7","DOIUrl":"https://doi.org/10.1186/s40104-025-01189-7","url":null,"abstract":"AFB1-8,9-exo-epoxide (AFBO) is the highly toxic product of Aflatoxin B1 (AFB1). Glutathione S-transferases (GSTs) play pivotal roles in detoxifying AFB1 by catalyzing the conjugation of AFBO with glutathione (GSH). Although there are over 20 GST isozymes that have been identified in chicken, GST isozymes involved in the detoxification process of AFB1 have not been identified yet. The objective of this study was to determine which GST isozymes played key role in detoxification of AFB1. A total of 17 pcDNA3.1(+)-GST isozyme plasmids were constructed and the GST isozyme genes were overexpressed by 80–2,500,000 folds in the chicken Leghorn male hepatoma (LMH) cells. Compared to the AFB1 treatment, overexpression of GSTA2X, GSTA3, GSTT1L, GSTZ1-1, and GSTZ1-2 increased the cell viability by 6.5%–17.0% in LMH cells. Moreover, overexpression of five GST isozymes reduced the release of lactate dehydrogenase and reactive oxygen species by 8.8%–64.4%, and 57.2%–77.6%, respectively, as well as enhanced the production AFBO-GSH by 15.8%–19.6%, thus mitigating DNA damage induced by AFB1. After comprehensive evaluation of various indicators, GSTA2X displayed the best detoxification effects against AFB1. GSTA2X was expressed in Pichia pastoris X-33 and its enzymatic properties for catalyzing the conjugation of AFBO with GSH showed that the optimum temperature and pH were 20–25 °C and 7.6–8.6 as well as the enzymatic kinetic parameter Vmax was 0.23 nmol/min/mg and the Michaelis constant was 86.05 μmol/L with the AFB1 as substrate. In conclusion, GSTA2X, GSTA3, GSTT1L, GSTZ1-1, and GSTZ1-2 played key roles in AFB1 detoxification, which will provide new remediation strategies to prevent aflatoxicosis in chickens.\u0000","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"23 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Regulating the regional deposition of fat is crucial for improving the carcass characteristics of pigs. The intestine, as an important organ for lipid absorption and homeostasis maintenance, secretes various biological signals that participate in the crosstalk between the intestine and adipose tissue. Extracellular vesicles, as novel extracellular genetic factors that mediate metabolic signal exchange among multiple tissues, have emerged as a hotspot and breakthrough in revealing the mechanisms of physiological homeostasis. However, how extracellular vesicles regulate the intestinal-adipose signaling axis, especially in relation lipid metabolism and deposition is still unclear. Thus, in the current study, intestinal extracellular vesicles from Chinese fat-type piglets of Lantang and typical lean-type piglets of Landrace were isolated and identified, and to reveal the regulatory mechanisms of lipid metabolism via intestinal extracellular vesicles in mediating intestinal-adipose crosstalk. We isolated and identified intestinal extracellular vesicles from the jejunum of 3-day-old Lantang and Landrace piglets (LT-EVs and LD-EVs) and further investigated their effects on lipid accumulation in porcine primary adipocytes. Compared to LD-EVs, LT-EVs promoted lipid deposition in porcine primary adipocytes, with intestinal-derived miRNAs playing a critical role in the crosstalk between the intestine and adipose tissue. Further analysis of extracellular vesicles-derived miRNA sequencing revealed that miR-30b-5p, enriched in LD-EVs, is involved in the regulation of lipid metabolism. Notably, the enrichment of miR-30b-5p in extracellular vesicles derived from IPEC-J2 cells also influenced lipid metabolism. Mechanistically, the targeted binding of miR-30b-5p and FMO3 may be critical for the extracellular vesicle-mediated regulation of lipid metabolism. Our findings suggest that jejunal-derived extracellular vesicles play a critical role in regulating lipid metabolism, and the regulatory effect of extracellular vesicles from obese piglets was higher than that of lean piglets. Furthermore, the different expression of miRNAs, such as miR-30b-5p, in intestinal extracellular vesicles may be the key to determining lipid deposition phenotypes across the two pig breeds.
{"title":"Porcine jejunal-derived extracellular vesicles participate in the regulation of lipid metabolism","authors":"Yaotian Fan, Haibin Deng, Jiahao Zhu, Junyi Luo, Ting Chen, Jiajie Sun, Yongliang Zhang, Qianyun Xi","doi":"10.1186/s40104-025-01185-x","DOIUrl":"https://doi.org/10.1186/s40104-025-01185-x","url":null,"abstract":"Regulating the regional deposition of fat is crucial for improving the carcass characteristics of pigs. The intestine, as an important organ for lipid absorption and homeostasis maintenance, secretes various biological signals that participate in the crosstalk between the intestine and adipose tissue. Extracellular vesicles, as novel extracellular genetic factors that mediate metabolic signal exchange among multiple tissues, have emerged as a hotspot and breakthrough in revealing the mechanisms of physiological homeostasis. However, how extracellular vesicles regulate the intestinal-adipose signaling axis, especially in relation lipid metabolism and deposition is still unclear. Thus, in the current study, intestinal extracellular vesicles from Chinese fat-type piglets of Lantang and typical lean-type piglets of Landrace were isolated and identified, and to reveal the regulatory mechanisms of lipid metabolism via intestinal extracellular vesicles in mediating intestinal-adipose crosstalk. We isolated and identified intestinal extracellular vesicles from the jejunum of 3-day-old Lantang and Landrace piglets (LT-EVs and LD-EVs) and further investigated their effects on lipid accumulation in porcine primary adipocytes. Compared to LD-EVs, LT-EVs promoted lipid deposition in porcine primary adipocytes, with intestinal-derived miRNAs playing a critical role in the crosstalk between the intestine and adipose tissue. Further analysis of extracellular vesicles-derived miRNA sequencing revealed that miR-30b-5p, enriched in LD-EVs, is involved in the regulation of lipid metabolism. Notably, the enrichment of miR-30b-5p in extracellular vesicles derived from IPEC-J2 cells also influenced lipid metabolism. Mechanistically, the targeted binding of miR-30b-5p and FMO3 may be critical for the extracellular vesicle-mediated regulation of lipid metabolism. Our findings suggest that jejunal-derived extracellular vesicles play a critical role in regulating lipid metabolism, and the regulatory effect of extracellular vesicles from obese piglets was higher than that of lean piglets. Furthermore, the different expression of miRNAs, such as miR-30b-5p, in intestinal extracellular vesicles may be the key to determining lipid deposition phenotypes across the two pig breeds. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-06DOI: 10.1186/s40104-025-01178-w
Mara Heckmann, Nadiia Sadova, Georg Sandner, Cathrina Neuhauser, Bernhard Blank-Landeshammer, Bettina Schwarzinger, Alice König, Meizhen Liang, Michael Spitzer, Julian Weghuber, Verena Stadlbauer
Maintaining intestinal health is crucial for the overall well-being and productivity of livestock, as it impacts nutrient absorption, immune function, and disease resistance. Oxidative stress and inflammation are key threats to intestinal integrity. This study explored the antioxidant, anti-inflammatory, and barrier-strengthening properties of a fermented plant macerate (FPM) derived from 45 local herbs, using a specifically developed fermentation process utilizing the plants’ inherent microbiota to enhance bioactivity and sustainability. In vitro experiments with IPEC-J2 cells showed that FPM significantly reduced intracellular reactive oxygen species (ROS) levels, improved barrier integrity, and enhanced cell migration under stress. Similar antioxidant effects were observed in THP-1 macrophages, where FPM reduced ROS production and modulated inflammatory responses by decreasing pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α), monokine induced by gamma interferon (MIG), interferon-inducible T cell alpha chemoattractant (I-TAC), macrophage inflammatory proteins (MIP)-1α and -1β] and increasing anti-inflammatory interleukin (IL)-10 levels. Mechanistic studies with HEK-Blue reporter cell lines revealed that FPM inhibited nuclear factor kappa B (NF-κB) activation via a toll-like receptor (TLR)4-independent pathway. In vivo, FPM significantly reduced ROS levels in Drosophila melanogaster and improved activity and LT50 values in Caenorhabditis elegans under oxidative stress, although it did not affect intestinal barrier integrity in these models. The findings indicate that FPM shows promising application as a functional feed supplement for improving intestinal health in livestock by mitigating oxidative stress and inflammation. Further studies, including livestock feeding trials, are recommended to validate these results.
{"title":"Herbal extract fermented with inherent microbiota improves intestinal health by exerting antioxidant and anti-inflammatory effects in vitro and in vivo","authors":"Mara Heckmann, Nadiia Sadova, Georg Sandner, Cathrina Neuhauser, Bernhard Blank-Landeshammer, Bettina Schwarzinger, Alice König, Meizhen Liang, Michael Spitzer, Julian Weghuber, Verena Stadlbauer","doi":"10.1186/s40104-025-01178-w","DOIUrl":"https://doi.org/10.1186/s40104-025-01178-w","url":null,"abstract":"Maintaining intestinal health is crucial for the overall well-being and productivity of livestock, as it impacts nutrient absorption, immune function, and disease resistance. Oxidative stress and inflammation are key threats to intestinal integrity. This study explored the antioxidant, anti-inflammatory, and barrier-strengthening properties of a fermented plant macerate (FPM) derived from 45 local herbs, using a specifically developed fermentation process utilizing the plants’ inherent microbiota to enhance bioactivity and sustainability. In vitro experiments with IPEC-J2 cells showed that FPM significantly reduced intracellular reactive oxygen species (ROS) levels, improved barrier integrity, and enhanced cell migration under stress. Similar antioxidant effects were observed in THP-1 macrophages, where FPM reduced ROS production and modulated inflammatory responses by decreasing pro-inflammatory cytokines [tumor necrosis factor alpha (TNF-α), monokine induced by gamma interferon (MIG), interferon-inducible T cell alpha chemoattractant (I-TAC), macrophage inflammatory proteins (MIP)-1α and -1β] and increasing anti-inflammatory interleukin (IL)-10 levels. Mechanistic studies with HEK-Blue reporter cell lines revealed that FPM inhibited nuclear factor kappa B (NF-κB) activation via a toll-like receptor (TLR)4-independent pathway. In vivo, FPM significantly reduced ROS levels in Drosophila melanogaster and improved activity and LT50 values in Caenorhabditis elegans under oxidative stress, although it did not affect intestinal barrier integrity in these models. The findings indicate that FPM shows promising application as a functional feed supplement for improving intestinal health in livestock by mitigating oxidative stress and inflammation. Further studies, including livestock feeding trials, are recommended to validate these results.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"23 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-05DOI: 10.1186/s40104-025-01187-9
Arianna N. Lopez, Makenzie G. Newton, Claire Stenhouse, Erin Connolly, Karina L. Hissen, Scott Horner, Guoyao Wu, William Foxworth, Fuller W. Bazer
Lactational performance depends heavily on age, health, and nutrition. L-Citrulline (Cit) is an effective precursor of L-arginine (Arg), an amino acid that has important roles in synthesis of nitric oxide (NO) and polyamines. Ruminal microbes degrade extracellular Arg; however, extracellular L-citrulline (Cit) is not degraded by ruminal microbes due to lack of uptake and can be fed unencapsulated as a precursor for Arg. As NO is a vasodilator, an increase in blood flow and transport of molecules to mammary tissue may enhance lactational performance and milk composition. Increases in polyamine production may increase milk protein synthesis within mammary tissue, thus increasing milk protein content. This study determined, for the first time, effects of dietary Cit supplementation on milk production and milk composition of Alpine dairy goats. Does were synchronized to estrus and bred to Alpine bucks. Parturition was induced on d 149 of gestation and does were suckled overnight allowing kid(s) to obtain colostrum before being milked 24 h later (d 1 of lactation). Does were assigned to either control (CON, n = 24) or Cit (CIT, n = 23) diets. The isonitrogenous control diet consisted of 97.63% basal diet and 2.37% supplement (1.37% L-alanine and 1.00% soybean hydrogenated oil). The CIT supplemented diet consisted of 97.63% basal diet and 2.37% supplement (0.5% Cit, 0.5% L-glutamine, 1% soybean hydrogenated oil, 0.37% cornstarch). Diets were group fed ad-libitum by treatment group. Blood samples were collected on d 0 and 30 of lactation, milk volumes measured twice daily, and on d 10, 20, and 40 of lactation, milk samples were collected. CIT-treated does had greater daily milk production (P < 0.05) and there was an effect of day of lactation on daily milk production (P < 0.0001). Sire had significant effect on daily milk production as well (P < 0.05). Milk compositional analyses revealed Cit supplementation increased solid-non-fat (SNF; P < 0.05) and protein (P < 0.05) content in milk. Our novel results indicate that dietary supplementation of Cit fed ad-libitum in Alpine does increased daily milk yield, milk SNF content, and protein content. Supplemental Cit may be a proxy for Arg in goats to enhance lactational performance.
泌乳性能在很大程度上取决于年龄、健康和营养。l -瓜氨酸（Cit）是l -精氨酸（Arg）的有效前体，在一氧化氮（NO）和多胺的合成中起重要作用。瘤胃微生物降解胞外精氨酸；然而，由于缺乏吸收，细胞外l -瓜氨酸（Cit）不会被瘤胃微生物降解，可以作为精氨酸的前体而不被包裹。由于一氧化氮是一种血管扩张剂，血流量的增加和分子向乳腺组织的运输可能会提高泌乳性能和乳成分。多胺产量的增加可能会增加乳腺组织内乳蛋白的合成，从而增加乳蛋白含量。本研究首次确定了饲粮中添加Cit对高寒奶山羊产奶量和乳成分的影响。它们与发情期同步，繁殖成阿尔卑斯雄鹿。在妊娠第149天诱导分娩，夜间哺乳，使患儿获得初乳，24 h后（哺乳期第1天）再挤奶。将小鼠分为对照组（CON, n = 24）和对照组（Cit, n = 23）。等氮对照饲粮为97.63%基础饲粮加2.37%补充饲粮（1.37% l -丙氨酸和1.00%大豆氢化油）。CIT补充饲粮为基础饲粮的97.63%和添加物（0.5% CIT、0.5% l -谷氨酰胺、1%大豆氢化油、0.37%玉米淀粉）的2.37%。治疗组随机分组饲喂。在泌乳第0天和第30天采集血液样本，每天测量两次产奶量，在泌乳第10、20和40天采集乳汁样本。cit处理的日产奶量显著高于对照组（P < 0.05），泌乳天数对日产奶量有显著影响（P < 0.0001）。父系对日产奶量也有显著影响（P < 0.05）。牛奶成分分析显示，添加Cit增加了固体非脂肪（SNF）；P < 0.05)和蛋白质含量（P < 0.05）。我们的新结果表明，在阿尔卑斯地区，饲粮中随意添加Cit确实提高了日产奶量、牛奶SNF含量和蛋白质含量。在山羊中添加精氨酸可能是提高泌乳性能的替代饲料。
{"title":"Dietary citrulline supplementation enhances milk production in lactating dairy goats","authors":"Arianna N. Lopez, Makenzie G. Newton, Claire Stenhouse, Erin Connolly, Karina L. Hissen, Scott Horner, Guoyao Wu, William Foxworth, Fuller W. Bazer","doi":"10.1186/s40104-025-01187-9","DOIUrl":"https://doi.org/10.1186/s40104-025-01187-9","url":null,"abstract":"Lactational performance depends heavily on age, health, and nutrition. L-Citrulline (Cit) is an effective precursor of L-arginine (Arg), an amino acid that has important roles in synthesis of nitric oxide (NO) and polyamines. Ruminal microbes degrade extracellular Arg; however, extracellular L-citrulline (Cit) is not degraded by ruminal microbes due to lack of uptake and can be fed unencapsulated as a precursor for Arg. As NO is a vasodilator, an increase in blood flow and transport of molecules to mammary tissue may enhance lactational performance and milk composition. Increases in polyamine production may increase milk protein synthesis within mammary tissue, thus increasing milk protein content. This study determined, for the first time, effects of dietary Cit supplementation on milk production and milk composition of Alpine dairy goats. Does were synchronized to estrus and bred to Alpine bucks. Parturition was induced on d 149 of gestation and does were suckled overnight allowing kid(s) to obtain colostrum before being milked 24 h later (d 1 of lactation). Does were assigned to either control (CON, n = 24) or Cit (CIT, n = 23) diets. The isonitrogenous control diet consisted of 97.63% basal diet and 2.37% supplement (1.37% L-alanine and 1.00% soybean hydrogenated oil). The CIT supplemented diet consisted of 97.63% basal diet and 2.37% supplement (0.5% Cit, 0.5% L-glutamine, 1% soybean hydrogenated oil, 0.37% cornstarch). Diets were group fed ad-libitum by treatment group. Blood samples were collected on d 0 and 30 of lactation, milk volumes measured twice daily, and on d 10, 20, and 40 of lactation, milk samples were collected. CIT-treated does had greater daily milk production (P < 0.05) and there was an effect of day of lactation on daily milk production (P < 0.0001). Sire had significant effect on daily milk production as well (P < 0.05). Milk compositional analyses revealed Cit supplementation increased solid-non-fat (SNF; P < 0.05) and protein (P < 0.05) content in milk. Our novel results indicate that dietary supplementation of Cit fed ad-libitum in Alpine does increased daily milk yield, milk SNF content, and protein content. Supplemental Cit may be a proxy for Arg in goats to enhance lactational performance.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143782485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-04DOI: 10.1186/s40104-025-01182-0
Xiaoran Feng, Yunlong Liu, Shengyang Xu, Junnan Ma, Hao Yuan, Haixin Wang, Jiachen Hu, Sijie Jin, Shanji Liu, Jin Zhong, Tao Ma, Yan Tu
Rumen microorganisms are key regulators of ruminant growth and production performance. Identifying probiotic candidates through microbial culturomics presents a promising strategy for improving ruminant production performance. Our previous study identified significant differences in rumen microbial communities of Holstein calves with varying average daily gain (ADG). This study aims to identify a target strain based on the findings from multi-omics analysis and literature review, isolating and evaluating the target microbial strains from both the rumen and hindgut contents for their probiotic potential. Parabacteroides distasonis, a strain closely associated with ADG, was successfully isolated from calf rumen content cultured with Fastidious Anaerobe Agar (FAA) medium and named Parabacteroides distasonis F4. Whole-genome sequencing and pan-genome analysis showed that P. distasonis F4 possesses a core functional potential for carbohydrate and amino acid metabolism, with the ability to produce propionate, acetate, and lactate. The results of targeted and untargeted metabolomics further validated the organic acid production and metabolic pathways of P. distasonis F4. An in vitro simulated rumen fermentation test showed that supplementation with P. distasonis F4 significantly altered rumen microbial community structure and increased the molar proportions of propionate and butyrate in the rumen. Furthermore, an in vivo study demonstrated that dietary supplementation with P. distasonis F4 significantly increased the ADG of pre-weaning calves. This study represents the first isolation of P. distasonis F4 from rumen, highlighting its potential as a probiotic strain for improving rumen development and growth performance in ruminants.
{"title":"Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation","authors":"Xiaoran Feng, Yunlong Liu, Shengyang Xu, Junnan Ma, Hao Yuan, Haixin Wang, Jiachen Hu, Sijie Jin, Shanji Liu, Jin Zhong, Tao Ma, Yan Tu","doi":"10.1186/s40104-025-01182-0","DOIUrl":"https://doi.org/10.1186/s40104-025-01182-0","url":null,"abstract":"Rumen microorganisms are key regulators of ruminant growth and production performance. Identifying probiotic candidates through microbial culturomics presents a promising strategy for improving ruminant production performance. Our previous study identified significant differences in rumen microbial communities of Holstein calves with varying average daily gain (ADG). This study aims to identify a target strain based on the findings from multi-omics analysis and literature review, isolating and evaluating the target microbial strains from both the rumen and hindgut contents for their probiotic potential. Parabacteroides distasonis, a strain closely associated with ADG, was successfully isolated from calf rumen content cultured with Fastidious Anaerobe Agar (FAA) medium and named Parabacteroides distasonis F4. Whole-genome sequencing and pan-genome analysis showed that P. distasonis F4 possesses a core functional potential for carbohydrate and amino acid metabolism, with the ability to produce propionate, acetate, and lactate. The results of targeted and untargeted metabolomics further validated the organic acid production and metabolic pathways of P. distasonis F4. An in vitro simulated rumen fermentation test showed that supplementation with P. distasonis F4 significantly altered rumen microbial community structure and increased the molar proportions of propionate and butyrate in the rumen. Furthermore, an in vivo study demonstrated that dietary supplementation with P. distasonis F4 significantly increased the ADG of pre-weaning calves. This study represents the first isolation of P. distasonis F4 from rumen, highlighting its potential as a probiotic strain for improving rumen development and growth performance in ruminants.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"80 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Caffeic acid (CA) and its derivative, chlorogenic acid (CGA), have shown promise in preventing and alleviating fatty liver disease. CA, compared to CGA, has much lower production costs and higher bioavailability, making it a potentially superior feed additive. However, the efficacy, mechanistic differences, and comparative impacts of CA and CGA on fatty liver disease in laying hens remain unclear. This study aimed to evaluate and compare the effects of CA and CGA on production performance, egg quality, and fatty liver disease in laying hens. A total of 1,440 61-week-old Hyline Brown laying hens were randomly divided into 8 groups and fed diets supplemented with basal diet, 25, 50, 100 and 200 mg/kg of CA, and 100, 200 and 400 mg/kg of CGA (CON, CA25, CA50, CA100, CA200, CGA100, CGA200 and CGA400, respectively) for 12 weeks. Both CA and CGA improved production performance and egg quality, while reducing markers of hepatic damage and lipid accumulation. CA and CGA significantly decreased TG, TC, and LDL-C levels and increased T-SOD activity. Transcriptomic and proteomic analyses revealed that CA and CGA reduced hepatic lipid accumulation through downregulation of lipid biosynthesis-related genes (ACLY, ACACA, FASN, and SCD1) and enhanced lipid transport and oxidation genes (FABPs, CD36, CPT1A, ACOX1, and SCP2). Of note, low-dose CA25 exhibited equivalent efficacy to the higher dose CGA100 group in alleviating fatty liver conditions. Mechanistically, CA and CGA alleviated lipid accumulation via activation of the ADPN-AMPK-PPARα signaling pathway. This study demonstrates that dietary CA and CGA effectively improve laying performance, egg quality, and hepatic lipid metabolism in laying hens, with CA potentially being more economical and efficient. Transcriptomic and proteomic evidence highlight shared mechanisms between CA25 and CGA100. These findings provide a foundation for CA and CGA as therapeutic agents for fatty liver disease and related metabolic diseases in hens, and also offer insights into the targeted modification of CGA (including the isomer of CGA) into CA, thereby providing novel strategies for the efficient utilization of CGA. (1) Dietary CA and CGA improve fatty liver, laying performance and egg quality. (2) Lower dose of CA25 achieves the equivalent improvement as CGA100 or CGA200. (3) CA and CGA mediate the ADPN-AMPK-PPARα pathway to alleviate fatty liver.
{"title":"Caffeic acid and chlorogenic acid mediate the ADPN-AMPK-PPARα pathway to improve fatty liver and production performance in laying hens","authors":"Wenjie Tian, Gerard Bryan Gonzales, Hao Wang, Youyou Yang, Chaohua Tang, Qingyu Zhao, Junmin Zhang, Huiyan Zhang, Yuchang Qin","doi":"10.1186/s40104-025-01175-z","DOIUrl":"https://doi.org/10.1186/s40104-025-01175-z","url":null,"abstract":"Caffeic acid (CA) and its derivative, chlorogenic acid (CGA), have shown promise in preventing and alleviating fatty liver disease. CA, compared to CGA, has much lower production costs and higher bioavailability, making it a potentially superior feed additive. However, the efficacy, mechanistic differences, and comparative impacts of CA and CGA on fatty liver disease in laying hens remain unclear. This study aimed to evaluate and compare the effects of CA and CGA on production performance, egg quality, and fatty liver disease in laying hens. A total of 1,440 61-week-old Hyline Brown laying hens were randomly divided into 8 groups and fed diets supplemented with basal diet, 25, 50, 100 and 200 mg/kg of CA, and 100, 200 and 400 mg/kg of CGA (CON, CA25, CA50, CA100, CA200, CGA100, CGA200 and CGA400, respectively) for 12 weeks. Both CA and CGA improved production performance and egg quality, while reducing markers of hepatic damage and lipid accumulation. CA and CGA significantly decreased TG, TC, and LDL-C levels and increased T-SOD activity. Transcriptomic and proteomic analyses revealed that CA and CGA reduced hepatic lipid accumulation through downregulation of lipid biosynthesis-related genes (ACLY, ACACA, FASN, and SCD1) and enhanced lipid transport and oxidation genes (FABPs, CD36, CPT1A, ACOX1, and SCP2). Of note, low-dose CA25 exhibited equivalent efficacy to the higher dose CGA100 group in alleviating fatty liver conditions. Mechanistically, CA and CGA alleviated lipid accumulation via activation of the ADPN-AMPK-PPARα signaling pathway. This study demonstrates that dietary CA and CGA effectively improve laying performance, egg quality, and hepatic lipid metabolism in laying hens, with CA potentially being more economical and efficient. Transcriptomic and proteomic evidence highlight shared mechanisms between CA25 and CGA100. These findings provide a foundation for CA and CGA as therapeutic agents for fatty liver disease and related metabolic diseases in hens, and also offer insights into the targeted modification of CGA (including the isomer of CGA) into CA, thereby providing novel strategies for the efficient utilization of CGA. (1) Dietary CA and CGA improve fatty liver, laying performance and egg quality. (2) Lower dose of CA25 achieves the equivalent improvement as CGA100 or CGA200. (3) CA and CGA mediate the ADPN-AMPK-PPARα pathway to alleviate fatty liver.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-02DOI: 10.1186/s40104-025-01188-8
Masroor Sagheer, McKenzie L. J. Haimon, Samuel Hincapie Montoya, Daniella Heredia, Federico Tarnonsky, Mauro E. Venturini, Angella Gonella-Diaza, Nicolas DiLorenzo, Joseph W. McFadden, Gabriela Dalmaso de Melo, Ky G. Pohler, Peter J. Hansen
Supplementation of choline chloride in culture medium programs the preimplantation bovine embryo to increase weaning weight of the resultant calf. Here, it was hypothesized that similar programming actions of choline can be induced by feeding rumen-protected choline (RPC) to beef cows during the periconceptional period. A preliminary experiment was conducted to determine changes in circulating concentrations of choline and its metabolites after RPC supplementation. Suckled beef cows were individually fed 0, 30, 60, and 90 g of RPC (i.e., 0, 8.6, 17.3 and 25.9 g choline chloride) and blood samples were collected at random times after feeding. There were no differences in plasma concentrations of choline or its metabolites between groups. In the second experiment, effects of feeding 60 g/d RPC from d −1 to 7 relative to timed artificial insemination were examined for suckled beef cows. Feeding RPC did not affect pregnancy or calving rates, pregnancy losses, plasma concentrations of pregnancy-associated glycoproteins, gestation length or calf birth weight. Calves from RPC fed dams were lighter than control calves at ~118 days of age (range 75–150; age included in the statistical model) and at weaning at ~248 days of age. There was no effect of treatment on hip height at ~118 days of age although there was a trend for RPC calves to be shorter at weaning. Weight/height ratio was lower for RPC than control at both 118 and 248 days of age. Treatment did not affect testis weight at ~118 days of age. Supplementation of RPC during the periconceptional period programmed development to alter calf phenotype in the postnatal period. The net result, reduced body weight, was the opposite of the phenotype caused by the addition of choline to embryo culture medium.
在培养基中添加氯化胆碱对着床前的牛胚胎进行编程，以增加犊牛断奶体重。本研究假设围孕期饲喂保护瘤胃胆碱（RPC）可以诱导类似的胆碱编程行为。初步实验确定了在补充RPC后循环胆碱及其代谢物浓度的变化。分别饲喂0、30、60和90 g RPC（分别为0、8.6、17.3和25.9 g氯化胆碱），饲喂后随机采集血液样本。各组间血浆胆碱及其代谢物浓度无差异。第二项试验研究了在第1 ~ 7天饲喂60 g/d RPC对哺乳肉牛人工授精的影响。饲喂RPC不影响妊娠率或产犊率、妊娠损失、妊娠相关糖蛋白的血浆浓度、妊娠期长度或小牛出生体重。RPC喂养的犊牛在~118日龄（75 ~ 150日龄）较对照犊牛轻；年龄包括在统计模型中)和~248日龄断奶时。治疗对~118日龄的臀高没有影响，尽管RPC犊牛在断奶时有变短的趋势。118日龄和248日龄RPC的体高比均低于对照组。治疗对~118日龄的睾丸重量没有影响。在妊娠期补充RPC可以改变产后小牛的表型。最终结果是体重减轻，这与在胚培养基中添加胆碱所引起的表型相反。
{"title":"Feeding rumen-protected choline during the periconceptional period programs postnatal phenotype of suckled beef calves","authors":"Masroor Sagheer, McKenzie L. J. Haimon, Samuel Hincapie Montoya, Daniella Heredia, Federico Tarnonsky, Mauro E. Venturini, Angella Gonella-Diaza, Nicolas DiLorenzo, Joseph W. McFadden, Gabriela Dalmaso de Melo, Ky G. Pohler, Peter J. Hansen","doi":"10.1186/s40104-025-01188-8","DOIUrl":"https://doi.org/10.1186/s40104-025-01188-8","url":null,"abstract":"Supplementation of choline chloride in culture medium programs the preimplantation bovine embryo to increase weaning weight of the resultant calf. Here, it was hypothesized that similar programming actions of choline can be induced by feeding rumen-protected choline (RPC) to beef cows during the periconceptional period. A preliminary experiment was conducted to determine changes in circulating concentrations of choline and its metabolites after RPC supplementation. Suckled beef cows were individually fed 0, 30, 60, and 90 g of RPC (i.e., 0, 8.6, 17.3 and 25.9 g choline chloride) and blood samples were collected at random times after feeding. There were no differences in plasma concentrations of choline or its metabolites between groups. In the second experiment, effects of feeding 60 g/d RPC from d −1 to 7 relative to timed artificial insemination were examined for suckled beef cows. Feeding RPC did not affect pregnancy or calving rates, pregnancy losses, plasma concentrations of pregnancy-associated glycoproteins, gestation length or calf birth weight. Calves from RPC fed dams were lighter than control calves at ~118 days of age (range 75–150; age included in the statistical model) and at weaning at ~248 days of age. There was no effect of treatment on hip height at ~118 days of age although there was a trend for RPC calves to be shorter at weaning. Weight/height ratio was lower for RPC than control at both 118 and 248 days of age. Treatment did not affect testis weight at ~118 days of age. Supplementation of RPC during the periconceptional period programmed development to alter calf phenotype in the postnatal period. The net result, reduced body weight, was the opposite of the phenotype caused by the addition of choline to embryo culture medium.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"64 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-01DOI: 10.1186/s40104-025-01170-4
Jonathan T. Baker, Zixiao Deng, Alexa R. Gormley, Sung Woo Kim
This study investigated the effects of different non-starch polysaccharide (NSP) sources with NSP degrading enzymes (NSPases) and the influence on the mucosa-associated microbiota and intestinal immunity of nursery pigs, on growth performance and carcass traits at market weight. One hundred and sixty newly weaned pigs at 7.0 ± 0.3 kg body weight (BW) were allotted in a 2 × 2 factorial with NSP sources and NSPases serving as factors. The 4 dietary treatments were: DDGS, corn distillers’ dried grains with solubles as source of NSP; DDGS + NSPases (DDGS +), DDGS with xylanase at 0.01%, 3,000 U/kg of feed and β-mannanase at 0.05%, 400 U/kg of feed; SHWB, soybean hulls and wheat bran replacing corn DDGS as the source of NSP; SHWB with NSPases (SHWB +), SHWB with xylanase at 0.01%, 3,000 U/kg of feed and β-mannanase at 0.05%, 400 U/kg of feed. Pigs were fed for 37 d and housed in groups of 4 pigs per pen. At d 37, the median body weight pig in each pen was euthanized for sampling to analyze intestinal health parameters. Remaining pigs were fed a common diet for subsequent phases to evaluate the carryover effect on growth and carcass traits. The SHWB decreased (P < 0.05) the relative abundance of Helicobacter, tended to increase (P = 0.074) the relative abundance of Lactobacillus, increased (P < 0.05) immunoglobulin G (IgG) in the jejunal mucosa, tended to increase (P = 0.096) the villus height (VH) in the jejunum, and tended to improve ADG (P = 0.099) and feed efficiency (P = 0.068) during phase 1 compared to DDGS treatment. Supplementation of NSPases increased (P < 0.05) Shannon index of diversity, increased the relative abundance of Streptococcus and Acinetobacter, and tended to increase (P = 0.082) dry matter digestibility. The BW of pigs fed SHWB was more uniform (P < 0.05) at the end of the 120 d study. Additionally, hot carcass weight of pigs fed SHWB tended to be more uniform (P = 0.089) than DDGS treatment. Soybean hulls and wheat bran replacing DDGS in nursery diets improved uniformity of pigs at market weight, which might be attributed to beneficial modulation of the mucosa-associated microbiota and enhanced intestinal morphology during the nursery phase. Supplementation of NSPases had beneficial effects on the intestinal mucosa-associated microbiota, digestibility, and intestinal immunity in SHWB treatment, whereas no carryover effects were overserved at market weight.
{"title":"Impacts of non-starch polysaccharide sources with enzymes influencing intestinal mucosa-associated microbiota and mucosal immunity of nursery pigs on growth and carcass traits at market weight","authors":"Jonathan T. Baker, Zixiao Deng, Alexa R. Gormley, Sung Woo Kim","doi":"10.1186/s40104-025-01170-4","DOIUrl":"https://doi.org/10.1186/s40104-025-01170-4","url":null,"abstract":"This study investigated the effects of different non-starch polysaccharide (NSP) sources with NSP degrading enzymes (NSPases) and the influence on the mucosa-associated microbiota and intestinal immunity of nursery pigs, on growth performance and carcass traits at market weight. One hundred and sixty newly weaned pigs at 7.0 ± 0.3 kg body weight (BW) were allotted in a 2 × 2 factorial with NSP sources and NSPases serving as factors. The 4 dietary treatments were: DDGS, corn distillers’ dried grains with solubles as source of NSP; DDGS + NSPases (DDGS +), DDGS with xylanase at 0.01%, 3,000 U/kg of feed and β-mannanase at 0.05%, 400 U/kg of feed; SHWB, soybean hulls and wheat bran replacing corn DDGS as the source of NSP; SHWB with NSPases (SHWB +), SHWB with xylanase at 0.01%, 3,000 U/kg of feed and β-mannanase at 0.05%, 400 U/kg of feed. Pigs were fed for 37 d and housed in groups of 4 pigs per pen. At d 37, the median body weight pig in each pen was euthanized for sampling to analyze intestinal health parameters. Remaining pigs were fed a common diet for subsequent phases to evaluate the carryover effect on growth and carcass traits. The SHWB decreased (P < 0.05) the relative abundance of Helicobacter, tended to increase (P = 0.074) the relative abundance of Lactobacillus, increased (P < 0.05) immunoglobulin G (IgG) in the jejunal mucosa, tended to increase (P = 0.096) the villus height (VH) in the jejunum, and tended to improve ADG (P = 0.099) and feed efficiency (P = 0.068) during phase 1 compared to DDGS treatment. Supplementation of NSPases increased (P < 0.05) Shannon index of diversity, increased the relative abundance of Streptococcus and Acinetobacter, and tended to increase (P = 0.082) dry matter digestibility. The BW of pigs fed SHWB was more uniform (P < 0.05) at the end of the 120 d study. Additionally, hot carcass weight of pigs fed SHWB tended to be more uniform (P = 0.089) than DDGS treatment. Soybean hulls and wheat bran replacing DDGS in nursery diets improved uniformity of pigs at market weight, which might be attributed to beneficial modulation of the mucosa-associated microbiota and enhanced intestinal morphology during the nursery phase. Supplementation of NSPases had beneficial effects on the intestinal mucosa-associated microbiota, digestibility, and intestinal immunity in SHWB treatment, whereas no carryover effects were overserved at market weight.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"33 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1186/s40104-025-01183-z
Zhibin Luo, Huimin Ou, Zhiliang Tan, Jinzhen Jiao
Dietary protein level and amino acid (AA) balance are crucial determinants of animal health and productivity. Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to improve the growth performance of ruminants. The colon serves as a crucial conduit for nutrient metabolism during rumen-protected methionine (RPMet) and rumen-protected lysine (RPLys) supplementation, however, it has been challenging to clarify which specific microbiota and their metabolites play a pivotal role in this process. Here, we applied metagenomic and metabolomic approaches to compare the characteristic microbiome and metabolic strategies in the colon of lambs fed a control diet (CON), a low-protein diet (LP) or a LP diet supplemented with RPMet and RPLys (LR). The LP treatment decreased the average daily weight gain (ADG) in lambs, while the LR treatment tended to elicit a remission in ADG. The butyrate molar concentration was greater (P < 0.05), while acetate molar concentration (P < 0.05) was lower for lambs fed the LP and LR diets compared to those fed the CON diet. Moreover, the LP treatment remarkably decreased total AA concentration (P < 0.05), while LR treatment showed an improvement in the concentrations of methionine, lysine, leucine, glutamate, and tryptophan. Metagenomic insights proved that the microbial metabolic potentials referring to biosynthesis of volatile fatty acids (VFAs) and AAs in the colon were remarkably altered by three dietary treatments. Metagenomic binning identified distinct microbial markers for the CON group (Alistipes spp., Phocaeicola spp., and Ruminococcus spp.), LP group (Fibrobacter spp., Prevotella spp., Ruminococcus spp., and Escherichia coli), and LR group (Akkermansia muciniphila and RUG099 spp.). Our findings suggest that RPMet and RPLys supplementation to the low-protein diet could enhance the microbial biosynthesis of butyrate and amino acids, enriche the beneficial bacteria in the colon, and thereby improve the growth performance of lambs.
{"title":"Rumen-protected methionine and lysine supplementation to the low protein diet improves animal growth through modulating colonic microbiome in lambs","authors":"Zhibin Luo, Huimin Ou, Zhiliang Tan, Jinzhen Jiao","doi":"10.1186/s40104-025-01183-z","DOIUrl":"https://doi.org/10.1186/s40104-025-01183-z","url":null,"abstract":"Dietary protein level and amino acid (AA) balance are crucial determinants of animal health and productivity. Supplementing rumen-protected AAs in low-protein diets was considered as an efficient strategy to improve the growth performance of ruminants. The colon serves as a crucial conduit for nutrient metabolism during rumen-protected methionine (RPMet) and rumen-protected lysine (RPLys) supplementation, however, it has been challenging to clarify which specific microbiota and their metabolites play a pivotal role in this process. Here, we applied metagenomic and metabolomic approaches to compare the characteristic microbiome and metabolic strategies in the colon of lambs fed a control diet (CON), a low-protein diet (LP) or a LP diet supplemented with RPMet and RPLys (LR). The LP treatment decreased the average daily weight gain (ADG) in lambs, while the LR treatment tended to elicit a remission in ADG. The butyrate molar concentration was greater (P < 0.05), while acetate molar concentration (P < 0.05) was lower for lambs fed the LP and LR diets compared to those fed the CON diet. Moreover, the LP treatment remarkably decreased total AA concentration (P < 0.05), while LR treatment showed an improvement in the concentrations of methionine, lysine, leucine, glutamate, and tryptophan. Metagenomic insights proved that the microbial metabolic potentials referring to biosynthesis of volatile fatty acids (VFAs) and AAs in the colon were remarkably altered by three dietary treatments. Metagenomic binning identified distinct microbial markers for the CON group (Alistipes spp., Phocaeicola spp., and Ruminococcus spp.), LP group (Fibrobacter spp., Prevotella spp., Ruminococcus spp., and Escherichia coli), and LR group (Akkermansia muciniphila and RUG099 spp.). Our findings suggest that RPMet and RPLys supplementation to the low-protein diet could enhance the microbial biosynthesis of butyrate and amino acids, enriche the beneficial bacteria in the colon, and thereby improve the growth performance of lambs.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"33 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><b>Correction: J Animal Sci Biotechnol 14, 45 (2023)</b></p><p><b>https://doi.org/10.1186/s40104-023–00852-1</b></p><br/><p>Following publication of the original article [1], the authors reported that Fig. 3 were incorrect because there was naming error occurred during the archiving of electron microscopy micrographs of mitochondrial ultrastructure for maternal Zn treatment referring to Zn + pbs and Zn + BHP groups, resulting in the incorrect use of these images in Fig. 3D. The other elements of the Fig. 3D remain the same, and the interpretation of the results remains unchanged. This error does not affect the conclusions drawn in the paper.</p><p>Figure 3 is corrected from:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 3</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-025-01179-9/MediaObjects/40104_2025_1179_Fig1_HTML.png?as=webp" type="image/webp"/><img alt="figure 1" aria-describedby="Fig1" height="477" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-025-01179-9/MediaObjects/40104_2025_1179_Fig1_HTML.png" width="685"/></picture><p>Maternal Zn addition attenuated in ovo injected BHP-induced mitochondrial dysfunction in embryo. The maternal Con and Zn groups diets were supplemented with either 0 or 220 mg Zn/kg diet for female broiler breeders. The embryos from Con and Zn groups were subjected to in ovo injection of either pbs or 600 μmol/L BHP on E14.<b> A</b> Effect maternal Zn addition on egg yolk Zn content. <b>B</b> Effect maternal Zn addition on hatchability performance. <b>C</b> Effect maternal Zn addition and in ovo injected BHP treatment on mitochondrial ROS and MMP. <b>D </b>Representative electron microscopy micrographs of mitochondrial ultrastructure. <b>E–G</b> Effect maternal Zn addition and in ovo injected BHP treatment on MDA, GSH, and MT4 contents in isolated cytoplasm and mitochondria. <b>H</b> Effect maternal Zn addition and in ovo injected BHP treatment on CuZnSOD activity in isolated cytoplasm. <b>I–J</b> Effect maternal Zn addition and in ovo injected BHP treatment on hepatic ATP content and mtDNA copy number. <b>K</b> and <b>L</b> Effect maternal Zn addition and in ovo injected BHP treatment on hepatic MT4, Nrf-2, PGC-1α, PPAR-α protein expressions. Graph bars in A and B were analyzed using unpaired two-tailed Student’s <i>t</i>-test (<sup><span>∗</span></sup><i>P</i> < 0.05, <i>n</i> = 6), while graph bars in <b>C</b>, <b>E</b>, <b>G</b>, <b>H</b>,<b> I</b> and<b> J</b> marked with different letters on top represent statistically significant results (<i>P </i>< 0.05, <i>n </i>= 4–6) based on Tukey’s post hoc analysis, whereas bars labelled with the same letter correspond to results that show no statistically significant differences. Data were mean ± SEM</p><span>Full size image</span><svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#i
中国恩平，恩平市，529400ChinaJu LiuAuthorsLiang HuangView Author publications您也可以在PubMed Google Scholar中搜索该作者Wei GaoView Author publications您也可以在PubMed Google Scholar中搜索该作者Xuri HeView Author publications您也可以在PubMed Google Scholar中搜索该作者TongYuanView作者发表的作品您也可以在PubMed Google Scholar中搜索该作者Huaqi ZhangView作者发表的作品您也可以在PubMed Google Scholar中搜索该作者Xiufen ZhangView作者发表的作品您也可以在PubMed Google Scholar中搜索该作者Wenxuan ZhengView作者发表的作品您也可以在PubMed Google Scholar中搜索该作者Wenxuan ZhengView作者发表的作品发表文章您也可以在 PubMed Google Scholar中搜索该作者Qilin Wu查看作者发表文章您也可以在 PubMed Google Scholar中搜索该作者Ju Liu查看作者发表文章您也可以在 PubMed Google Scholar中搜索该作者Wence Wang查看作者发表文章您也可以在 PubMed Google Scholar中搜索该作者在PubMed Google Scholar中搜索该作者杨林查看作者发表的论文您也可以在 PubMed Google Scholar中搜索该作者朱永文查看作者发表的论文您也可以在 PubMed Google Scholar中搜索该作者通讯作者：杨林或朱永文。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但须注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。如需查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明 (http://creativecommons.org/publicdomain/zero/1.0/) 适用于本文提供的数据。转载与许可引用本文Huang, L., Gao, W., He, X. et al. Correction：母体锌通过激活Nrf2/PGC-1α途径缓解叔丁基过氧化氢诱导的线粒体氧化应激对胚胎发育的影响。J Animal Sci Biotechnol 16, 45 (2025). https://doi.org/10.1186/s40104-025-01179-9Download citationPublished: 17 March 2025DOI: https://doi.org/10.1186/s40104-025-01179-9Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative.
{"title":"Correction: Maternal zinc alleviates tert-butyl hydroperoxide-induced mitochondrial oxidative stress on embryonic development involving the activation of Nrf2/PGC-1α pathway","authors":"Liang Huang, Wei Gao, Xuri He, Tong Yuan, Huaqi Zhang, Xiufen Zhang, Wenxuan Zheng, Qilin Wu, Ju Liu, Wence Wang, Lin Yang, Yongwen Zhu","doi":"10.1186/s40104-025-01179-9","DOIUrl":"https://doi.org/10.1186/s40104-025-01179-9","url":null,"abstract":"<p><b>Correction: J Animal Sci Biotechnol 14, 45 (2023)</b></p><p><b>https://doi.org/10.1186/s40104-023–00852-1</b></p><br/><p>Following publication of the original article [1], the authors reported that Fig. 3 were incorrect because there was naming error occurred during the archiving of electron microscopy micrographs of mitochondrial ultrastructure for maternal Zn treatment referring to Zn + pbs and Zn + BHP groups, resulting in the incorrect use of these images in Fig. 3D. The other elements of the Fig. 3D remain the same, and the interpretation of the results remains unchanged. This error does not affect the conclusions drawn in the paper.</p><p>Figure 3 is corrected from:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 3</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-025-01179-9/MediaObjects/40104_2025_1179_Fig1_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 1\" aria-describedby=\"Fig1\" height=\"477\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-025-01179-9/MediaObjects/40104_2025_1179_Fig1_HTML.png\" width=\"685\"/></picture><p>Maternal Zn addition attenuated in ovo injected BHP-induced mitochondrial dysfunction in embryo. The maternal Con and Zn groups diets were supplemented with either 0 or 220 mg Zn/kg diet for female broiler breeders. The embryos from Con and Zn groups were subjected to in ovo injection of either pbs or 600 μmol/L BHP on E14.<b> A</b> Effect maternal Zn addition on egg yolk Zn content. <b>B</b> Effect maternal Zn addition on hatchability performance. <b>C</b> Effect maternal Zn addition and in ovo injected BHP treatment on mitochondrial ROS and MMP. <b>D </b>Representative electron microscopy micrographs of mitochondrial ultrastructure. <b>E–G</b> Effect maternal Zn addition and in ovo injected BHP treatment on MDA, GSH, and MT4 contents in isolated cytoplasm and mitochondria. <b>H</b> Effect maternal Zn addition and in ovo injected BHP treatment on CuZnSOD activity in isolated cytoplasm. <b>I–J</b> Effect maternal Zn addition and in ovo injected BHP treatment on hepatic ATP content and mtDNA copy number. <b>K</b> and <b>L</b> Effect maternal Zn addition and in ovo injected BHP treatment on hepatic MT4, Nrf-2, PGC-1α, PPAR-α protein expressions. Graph bars in A and B were analyzed using unpaired two-tailed Student’s <i>t</i>-test (<sup><span>∗</span></sup><i>P</i> < 0.05, <i>n</i> = 6), while graph bars in <b>C</b>, <b>E</b>, <b>G</b>, <b>H</b>,<b> I</b> and<b> J</b> marked with different letters on top represent statistically significant results (<i>P </i>< 0.05, <i>n </i>= 4–6) based on Tukey’s post hoc analysis, whereas bars labelled with the same letter correspond to results that show no statistically significant differences. Data were mean ± SEM</p><span>Full size image</span><svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#i","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"199 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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