<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 128 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01088-3</b></p><br/><p>Following publication of the original article [1], the authors reported that in the original Fig. 3A, on W14, the pictures stained by DHE and DAPI fluorochrome, and the merged picture were placed out of order.</p><p>The original Fig. 3 was:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 3</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png?as=webp" type="image/webp"/><img alt="figure 1" aria-describedby="Fig1" height="465" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png" width="685"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; CAT: Catalase; GPX: Glutathione peroxidase; MDA: Malonaldehyde</p><span>Full size image</span><svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-chevron-right-small" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></figure><p>The correct Fig. 3 should be:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 3</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png?as=webp" type="image/webp"/><img alt="figure 2" aria-describedby="Fig2" height="494" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png" width="685"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase;
更正：J Animal Sci Biotechnol 15, 128 (2024)https://doi.org/10.1186/s40104-024-01088-3Following 原文[1]发表后，作者报告原图3A中W14上DHE和DAPI荧光染色的图片以及合并后的图片放置顺序有误。原图3为：图3肝脏氧化还原参数。A 和 B 冷冻肝脏切片的 DHE 染色和 ROS 的荧光强度；C-E 肝脏 H2O2、O2- 和 T-AOC 含量；F-H 肝脏 T-SOD、CAT 和 GPX 活性；I 肝脏 MDA 含量。W0、W1、W4、W7 和 W14 分别代表 21、22、25、28 和 35 天龄。数据以平均值 ± SEM 表示（ROS，n = 3；其他，n = 6）。不同字母的数值差异显著（P < 0.05）。ROS：活性氧；T-AOC：总抗氧化能力；T-SOD：总超氧化物歧化酶；CAT：过氧化氢酶；GPX：谷胱甘肽过氧化物酶；MDA：丙二醛：全图正确的图 3 应该是：图 3 肝脏氧化还原参数。A 和 B 冷冻肝脏切片的 DHE 染色和 ROS 的荧光强度；C-E 肝脏 H2O2、O2- 和 T-AOC 含量；F-H 肝脏 T-SOD、CAT 和 GPX 活性；I 肝脏 MDA 含量。W0、W1、W4、W7 和 W14 分别代表 21、22、25、28 和 35 天龄。数据以平均值 ± SEM 表示（ROS，n = 3；其他，n = 6）。不同字母的数值差异显著（P < 0.05）。ROS：活性氧；T-AOC：总抗氧化能力；T-SOD：总超氧化物歧化酶；CAT：过氧化氢酶；GPX：谷胱甘肽过氧化物酶；MDA：丙二醛：Yu C, Luo Y, Shen C, et al. 微生物衍生抗氧化剂对断奶仔猪生长性能、肝脏氧化应激、线粒体功能和细胞凋亡的影响。J Anim Sci Biotechnol.2024;15:128. https://doi.org/10.1186/s40104-024-01088-3.Article PubMed PubMed Central Google Scholar Download references作者及单位上海交通大学农业与生物学院兽医生物技术上海市重点实验室，上海，200240Chengbing Yu，Yuxiao Luo，Cheng Shen，Zhen Luo，Hongcai Zhang，Jing Zhang，Weina Xu &；Jianxiong Xu作者：Chengbing Yu查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Yuxiao Luo查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Cheng Shen查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Zhen Luo查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Hongcai Zhang查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Hongcai Zhang查看作者发表的论文您也可以在PubMed Google Scholar中搜索Hongcai Zhang查看作者发表的论文发表文章您也可以在 PubMed Google Scholar中搜索该作者张静发表文章您也可以在 PubMed Google Scholar中搜索该作者徐伟娜发表文章您也可以在 PubMed Google Scholar中搜索该作者徐建雄发表文章您也可以在 PubMed Google Scholar中搜索该作者通讯作者：徐建雄。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但必须注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明 (http://creativecommons.org/publicdomain/zero/1.0/) 适用于本文提供的数据。转载与许可引用本文Yu, C., Luo, Y., Shen, C. et al. Correction：微生物源抗氧化剂对断奶仔猪生长性能、肝脏氧化应激、线粒体功能和细胞凋亡的影响。J Animal Sci Biotechnol 15, 151 (2024). https://doi.org/10.1186/s40104-024-01116-2Download citationPublished: 04 November 2024DOI: https://doi.org/10.1186/s40104-024-01116-2Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative
{"title":"Correction: Effects of microbe-derived antioxidants on growth performance, hepatic oxidative stress, mitochondrial function and cell apoptosis in weaning piglets","authors":"Chengbing Yu, Yuxiao Luo, Cheng Shen, Zhen Luo, Hongcai Zhang, Jing Zhang, Weina Xu, Jianxiong Xu","doi":"10.1186/s40104-024-01116-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01116-2","url":null,"abstract":"<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 128 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01088-3</b></p><br/><p>Following publication of the original article [1], the authors reported that in the original Fig. 3A, on W14, the pictures stained by DHE and DAPI fluorochrome, and the merged picture were placed out of order.</p><p>The original Fig. 3 was:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 3</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 1\" aria-describedby=\"Fig1\" height=\"465\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig1_HTML.png\" width=\"685\"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; CAT: Catalase; GPX: Glutathione peroxidase; MDA: Malonaldehyde</p><span>Full size image</span><svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-chevron-right-small\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></figure><p>The correct Fig. 3 should be:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 3</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 2\" aria-describedby=\"Fig2\" height=\"494\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01116-2/MediaObjects/40104_2024_1116_Fig2_HTML.png\" width=\"685\"/></picture><p>The hepatic redox parameters. <b>A</b> and <b>B</b> DHE Staining with frozen liver sections and fluorescence intensity of ROS; <b>C</b>–<b>E</b> Hepatic H<sub>2</sub>O<sub>2</sub>, O<sub>2</sub><sup>−</sup> and T-AOC content; <b>F</b>–<b>H</b> Hepatic T-SOD, CAT and GPX activity; <b>I</b> Hepatic MDA content. W0, W1, W4, W7, and W14 respectively represented 21, 22, 25, 28, and 35 days of age. Data were presented as mean ± SEM (ROS, <i>n</i> = 3; others, <i>n</i> = 6). Values with different letters differ significantly (<i>P</i> < 0.05). ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; T-SOD: Total superoxide dismutase; ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-02DOI: 10.1186/s40104-024-01105-5
Hemlata Gautam, Noor Ahmad Shaik, Babajan Banaganapalli, Shelly Popowich, Iresha Subhasinghe, Lisanework E. Ayalew, Rupasri Mandal, David S. Wishart, Suresh Tikoo, Susantha Gomis
Necrotic enteritis (NE) is an economically important disease of broiler chickens caused by Clostridium perfringens (CP). The pathogenesis, or disease process, of NE is still not clear. This study aimed to identify the alterations of metabolites and metabolic pathways associated with subclinical or clinical NE in CP infected birds and to investigate the possible variations in the metabolic profile of birds infected with different isolates of CP. Using a well-established NE model, the protein content of feed was changed abruptly before exposing birds to CP isolates with different toxin genes combinations (cpa, cpb2, netB, tpeL; cpa, cpb2, netB; or cpa, cpb2). Metabolomics analysis of jejunal contents was performed by a targeted, fully quantitative LC-MS/MS based assay. This study detected statistically significant differential expression of 34 metabolites including organic acids, amino acids, fatty acids, and biogenic amines, including elevation of butyric acid at onset of NE in broiler chickens. Subsequent analysis of broilers infected with CP isolates with different toxin gene combinations confirmed an elevation of butyric acid consistently among 21 differentially expressed metabolites including organic acids, amino acids, and biogenic amines, underscoring its potential role during the development of NE. Furthermore, protein-metabolite network analysis revealed significant alterations in butyric acid and arginine-proline metabolisms. This study indicates a significant metabolic difference between CP-infected and non-infected broiler chickens. Among all the metabolites, butyric acid increased significantly in CP-infected birds compared to non-infected healthy broilers. Logistic regression analysis revealed a positive association between butyric acid (coefficient: 1.23, P < 0.01) and CP infection, while showing a negative association with amino acid metabolism. These findings suggest that butyric acid could be a crucial metabolite linked to the occurrence of NE in broiler chickens and may serve as an early indicator of the disease at the farm level. Further metabolomic experiments using different NE animal models and field studies are needed to determine the specificity and to validate metabolites associated with NE, regardless of predisposing factors.
坏死性肠炎（NE）是由产气荚膜梭菌（CP）引起的肉鸡的一种重要经济疾病。坏死性肠炎的发病机制或疾病过程尚不清楚。本研究旨在确定受 CP 感染的禽类体内与亚临床或临床 NE 相关的代谢物和代谢途径的变化，并调查受不同 CP 分离物感染的禽类在代谢方面可能存在的变化。利用一个成熟的NE模型，在鸟类接触具有不同毒素基因组合（cpa、cpb2、netB、tpeL；cpa、cpb2、netB；或cpa、cpb2）的CP分离物之前，突然改变饲料中的蛋白质含量。空肠内容物的代谢组学分析是通过一种基于靶向、完全定量的 LC-MS/MS 方法进行的。该研究检测到 34 种代谢物（包括有机酸、氨基酸、脂肪酸和生物胺）存在统计学意义上的差异表达，其中包括肉鸡 NE 发病时丁酸的升高。随后对感染了具有不同毒素基因组合的 CP 分离物的肉鸡进行的分析证实，在包括有机酸、氨基酸和生物胺在内的 21 种差异表达代谢物中，丁酸的表达量一直在升高，这突显了丁酸在 NE 发生过程中的潜在作用。此外，蛋白质代谢物网络分析显示，丁酸和精氨酸-脯氨酸代谢发生了显著变化。这项研究表明，感染氯化石蜡的肉鸡与未感染氯化石蜡的肉鸡在代谢方面存在明显差异。在所有代谢物中，与未感染 CP 的健康肉鸡相比，感染 CP 的肉鸡体内的丁酸明显增加。逻辑回归分析表明，丁酸（系数：1.23，P < 0.01）与CP感染呈正相关，而与氨基酸代谢呈负相关。这些研究结果表明，丁酸可能是与肉鸡NE发生相关的重要代谢物，可作为鸡场的早期疾病指标。需要利用不同的 NE 动物模型和现场研究开展进一步的代谢组学实验，以确定特异性并验证与 NE 相关的代谢物，而不考虑易感因素。
{"title":"Elevated levels of butyric acid in the jejunum of an animal model of broiler chickens: from early onset of Clostridium perfringens infection to clinical disease of necrotic enteritis","authors":"Hemlata Gautam, Noor Ahmad Shaik, Babajan Banaganapalli, Shelly Popowich, Iresha Subhasinghe, Lisanework E. Ayalew, Rupasri Mandal, David S. Wishart, Suresh Tikoo, Susantha Gomis","doi":"10.1186/s40104-024-01105-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01105-5","url":null,"abstract":"Necrotic enteritis (NE) is an economically important disease of broiler chickens caused by Clostridium perfringens (CP). The pathogenesis, or disease process, of NE is still not clear. This study aimed to identify the alterations of metabolites and metabolic pathways associated with subclinical or clinical NE in CP infected birds and to investigate the possible variations in the metabolic profile of birds infected with different isolates of CP. Using a well-established NE model, the protein content of feed was changed abruptly before exposing birds to CP isolates with different toxin genes combinations (cpa, cpb2, netB, tpeL; cpa, cpb2, netB; or cpa, cpb2). Metabolomics analysis of jejunal contents was performed by a targeted, fully quantitative LC-MS/MS based assay. This study detected statistically significant differential expression of 34 metabolites including organic acids, amino acids, fatty acids, and biogenic amines, including elevation of butyric acid at onset of NE in broiler chickens. Subsequent analysis of broilers infected with CP isolates with different toxin gene combinations confirmed an elevation of butyric acid consistently among 21 differentially expressed metabolites including organic acids, amino acids, and biogenic amines, underscoring its potential role during the development of NE. Furthermore, protein-metabolite network analysis revealed significant alterations in butyric acid and arginine-proline metabolisms. This study indicates a significant metabolic difference between CP-infected and non-infected broiler chickens. Among all the metabolites, butyric acid increased significantly in CP-infected birds compared to non-infected healthy broilers. Logistic regression analysis revealed a positive association between butyric acid (coefficient: 1.23, P < 0.01) and CP infection, while showing a negative association with amino acid metabolism. These findings suggest that butyric acid could be a crucial metabolite linked to the occurrence of NE in broiler chickens and may serve as an early indicator of the disease at the farm level. Further metabolomic experiments using different NE animal models and field studies are needed to determine the specificity and to validate metabolites associated with NE, regardless of predisposing factors.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"141 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus). Expose Nile tilapia to alkaline water of different alkalinity for 2 weeks to observe changes in its growth performance and proline metabolism. Meanwhile, to further clarify the role of proline metabolism, RNA interference experiments were conducted to disrupt the normal operation of proline metabolic axis by knocking down pycr (pyrroline-5-carboxylate reductases), the final rate-limiting enzyme in proline synthesis. The results showed that both the synthesis and degradation of proline were enhanced under carbonate alkalinity stress, and the environmental alkalinity impaired the growth performance of tilapia, and the higher the alkalinity, the greater the impairment. Moreover, environmental alkalinity caused oxidative stress in tilapia, enhanced ion transport, ammonia metabolism, and altered the intensity and form of energy metabolism in tilapia. When the expression level of the pycr gene decreased, the proline metabolism could not operate normally, and the ion transport, antioxidant defense system, and energy metabolism were severely damaged, ultimately leading to liver damage and a decreased survival rate of tilapia under alkalinity stress. The results indicated that proline metabolism plays an important role in the alkaline adaptation of Nile tilapia and is a key regulatory process in various biochemical and physiological processes.
{"title":"Proline metabolism is essential for alkaline adaptation of Nile tilapia (Oreochromis niloticus)","authors":"Minxu Wang, Yuxi Yan, Wei Liu, Jinquan Fan, Erchao Li, Liqiao Chen, Xiaodan Wang","doi":"10.1186/s40104-024-01100-w","DOIUrl":"https://doi.org/10.1186/s40104-024-01100-w","url":null,"abstract":"Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus). Expose Nile tilapia to alkaline water of different alkalinity for 2 weeks to observe changes in its growth performance and proline metabolism. Meanwhile, to further clarify the role of proline metabolism, RNA interference experiments were conducted to disrupt the normal operation of proline metabolic axis by knocking down pycr (pyrroline-5-carboxylate reductases), the final rate-limiting enzyme in proline synthesis. The results showed that both the synthesis and degradation of proline were enhanced under carbonate alkalinity stress, and the environmental alkalinity impaired the growth performance of tilapia, and the higher the alkalinity, the greater the impairment. Moreover, environmental alkalinity caused oxidative stress in tilapia, enhanced ion transport, ammonia metabolism, and altered the intensity and form of energy metabolism in tilapia. When the expression level of the pycr gene decreased, the proline metabolism could not operate normally, and the ion transport, antioxidant defense system, and energy metabolism were severely damaged, ultimately leading to liver damage and a decreased survival rate of tilapia under alkalinity stress. The results indicated that proline metabolism plays an important role in the alkaline adaptation of Nile tilapia and is a key regulatory process in various biochemical and physiological processes.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"229 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-13DOI: 10.1186/s40104-024-01103-7
Sangwoo Park, Shuhan Sun, Lauren Kovanda, Adebayo O. Sokale, Adriana Barri, Kwangwook Kim, Xunde Li, Yanhong Liu
Monoglycerides have emerged as a promising alternative to conventional practices due to their biological activities, including antimicrobial properties. However, few studies have assessed the efficacy of monoglyceride blend on weaned pigs and their impacts on performance, immune response, and gut health using a disease challenge model. Therefore, this study aimed to investigate the effects of dietary monoglycerides of short- and medium-chain fatty acids on the immunity and gut health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli F18. Pigs supplemented with high-dose zinc oxide (ZNO) had greater (P < 0.05) growth performance than other treatments, but no difference was observed in average daily feed intake between ZNO and monoglycerides groups during the post-challenge period. Pigs in ZNO and antibiotic groups had lower (P < 0.05) severity of diarrhea than control, but the severity of diarrhea was not different between antibiotic and monoglycerides groups. Pigs fed with monoglycerides or ZNO had lower (P < 0.05) serum haptoglobin on d 2 or 5 post-inoculation than control. Pigs in ZNO had greater (P < 0.05) goblet cell numbers per villus, villus area and height, and villus height:crypt depth ratio (VH:CD) in duodenum on d 5 post-inoculation than pigs in other treatments. Pigs supplemented with monoglycerides, ZNO, or antibiotics had reduced (P < 0.05) ileal crypt depth compared with control on d 5 post-inoculation, contributing to the increase (P = 0.06) in VH:CD. Consistently, pigs in ZNO expressed the lowest (P < 0.05) TNFa, IL6, IL10, IL12, IL1A, IL1B, and PTGS2 in ileal mucosa on d 5 post-inoculation, and no difference was observed in the expression of those genes between ZNO and monoglycerides. Supplementation of ZNO and antibiotic had significant impacts on metabolic pathways in the serum compared with control, particularly on carbohydrate and amino acid metabolism, while limited impacts on serum metabolites were observed in monoglycerides group when compared with control. The results suggest that supplementation of monoglyceride blend may enhance disease resistance of weaned pigs by alleviating the severity of diarrhea and mitigating intestinal and systemic inflammation, although the effectiveness may not be comparable to high-dose zinc oxide.
{"title":"Effects of monoglyceride blend on systemic and intestinal immune responses, and gut health of weaned pigs experimentally infected with a pathogenic Escherichia coli","authors":"Sangwoo Park, Shuhan Sun, Lauren Kovanda, Adebayo O. Sokale, Adriana Barri, Kwangwook Kim, Xunde Li, Yanhong Liu","doi":"10.1186/s40104-024-01103-7","DOIUrl":"https://doi.org/10.1186/s40104-024-01103-7","url":null,"abstract":"Monoglycerides have emerged as a promising alternative to conventional practices due to their biological activities, including antimicrobial properties. However, few studies have assessed the efficacy of monoglyceride blend on weaned pigs and their impacts on performance, immune response, and gut health using a disease challenge model. Therefore, this study aimed to investigate the effects of dietary monoglycerides of short- and medium-chain fatty acids on the immunity and gut health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli F18. Pigs supplemented with high-dose zinc oxide (ZNO) had greater (P < 0.05) growth performance than other treatments, but no difference was observed in average daily feed intake between ZNO and monoglycerides groups during the post-challenge period. Pigs in ZNO and antibiotic groups had lower (P < 0.05) severity of diarrhea than control, but the severity of diarrhea was not different between antibiotic and monoglycerides groups. Pigs fed with monoglycerides or ZNO had lower (P < 0.05) serum haptoglobin on d 2 or 5 post-inoculation than control. Pigs in ZNO had greater (P < 0.05) goblet cell numbers per villus, villus area and height, and villus height:crypt depth ratio (VH:CD) in duodenum on d 5 post-inoculation than pigs in other treatments. Pigs supplemented with monoglycerides, ZNO, or antibiotics had reduced (P < 0.05) ileal crypt depth compared with control on d 5 post-inoculation, contributing to the increase (P = 0.06) in VH:CD. Consistently, pigs in ZNO expressed the lowest (P < 0.05) TNFa, IL6, IL10, IL12, IL1A, IL1B, and PTGS2 in ileal mucosa on d 5 post-inoculation, and no difference was observed in the expression of those genes between ZNO and monoglycerides. Supplementation of ZNO and antibiotic had significant impacts on metabolic pathways in the serum compared with control, particularly on carbohydrate and amino acid metabolism, while limited impacts on serum metabolites were observed in monoglycerides group when compared with control. The results suggest that supplementation of monoglyceride blend may enhance disease resistance of weaned pigs by alleviating the severity of diarrhea and mitigating intestinal and systemic inflammation, although the effectiveness may not be comparable to high-dose zinc oxide.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-09DOI: 10.1186/s40104-024-01097-2
Jean M. Feugang, Ahmed Gad, Nico G. Menjivar, Ghassan M. Ishak, Samuel Gebremedhn, Melba O. Gastal, Notsile H. Dlamini, Radek Prochazka, Eduardo L. Gastal, Dawit Tesfaye
Ovarian follicular fluid (FF) is a dynamic environment that changes with the seasons, affecting follicle development, ovulation, and oocyte quality. Cells in the follicles release tiny particles called extracellular vesicles (EVs) containing vital regulatory molecules, such as microRNAs (miRNAs). These miRNAs are pivotal in facilitating communication within the follicles through diverse signaling and information transfer forms. EV-coupled miRNA signaling is implicated to be associated with ovarian function, follicle and oocyte growth and response to various environmental insults. Herein, we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses. Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the anovulatory (spring: non-breeding) and ovulatory (spring, summer, and fall: breeding) seasons and subsequent EV isolation and miRNA profiling identified significant variation in EV-miRNA cargo content. We identified 97 miRNAs with differential expression among the groups and specific clusters of miRNAs involved in the spring transition (miR-149, -200b, -206, -221, -328, and -615) and peak breeding period (including miR-143, -192, -451, -302b, -100, and let-7c). Bioinformatic analyses showed enrichments in various biological functions, e.g., transcription factor activity, transcription and transcription regulation, nucleic acid binding, sequence-specific DNA binding, p53 signaling, and post-translational modifications. Cluster analyses revealed distinct sets of significantly up- and down-regulated miRNAs associated with spring anovulatory (Cluster 1) and summer ovulation–the peak breeding season (Clusters 4 and 6). The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons, and their roles in understanding the mechanisms involved in seasonal shifts and ovulation during the breeding season warrant further investigation.
{"title":"Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid","authors":"Jean M. Feugang, Ahmed Gad, Nico G. Menjivar, Ghassan M. Ishak, Samuel Gebremedhn, Melba O. Gastal, Notsile H. Dlamini, Radek Prochazka, Eduardo L. Gastal, Dawit Tesfaye","doi":"10.1186/s40104-024-01097-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01097-2","url":null,"abstract":"Ovarian follicular fluid (FF) is a dynamic environment that changes with the seasons, affecting follicle development, ovulation, and oocyte quality. Cells in the follicles release tiny particles called extracellular vesicles (EVs) containing vital regulatory molecules, such as microRNAs (miRNAs). These miRNAs are pivotal in facilitating communication within the follicles through diverse signaling and information transfer forms. EV-coupled miRNA signaling is implicated to be associated with ovarian function, follicle and oocyte growth and response to various environmental insults. Herein, we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses. Ultrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the anovulatory (spring: non-breeding) and ovulatory (spring, summer, and fall: breeding) seasons and subsequent EV isolation and miRNA profiling identified significant variation in EV-miRNA cargo content. We identified 97 miRNAs with differential expression among the groups and specific clusters of miRNAs involved in the spring transition (miR-149, -200b, -206, -221, -328, and -615) and peak breeding period (including miR-143, -192, -451, -302b, -100, and let-7c). Bioinformatic analyses showed enrichments in various biological functions, e.g., transcription factor activity, transcription and transcription regulation, nucleic acid binding, sequence-specific DNA binding, p53 signaling, and post-translational modifications. Cluster analyses revealed distinct sets of significantly up- and down-regulated miRNAs associated with spring anovulatory (Cluster 1) and summer ovulation–the peak breeding season (Clusters 4 and 6). The findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons, and their roles in understanding the mechanisms involved in seasonal shifts and ovulation during the breeding season warrant further investigation.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"67 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chronic heat stress (CHS) is a detrimental environmental stressor with a negative impact on the meat quality of broilers. However, the underlying mechanisms are not fully understood. This study investigates the effects of CHS on long non-coding RNA (lncRNA) expression and muscle injury in broilers, with a focus on its implications for meat quality. The results showed that CHS diminished breast muscle yield, elevated abdominal fat deposition, induced cellular apoptosis (P < 0.05), and caused myofibrosis. Transcriptomic analysis revealed 151 differentially expressed (DE) lncRNAs when comparing the normal control (NC) and HS groups, 214 DE lncRNAs when comparing the HS and PF groups, and 79 DE lncRNAs when comparing the NC and pair-fed (PF) groups. After eliminating the confounding effect of feed intake, 68 lncRNAs were identified, primarily associated with cellular growth and death, signal transduction, and metabolic regulation. Notably, the apoptosis-related pathway P53, lysosomes, and the fibrosis-related gene TGF-β2 were significantly upregulated by lncRNAs. These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA, leading to connective tissue accumulation, which likely contributes to reduced breast muscle yield and meat quality in broilers.
{"title":"Unraveling the role of long non-coding RNAs in chronic heat stress-induced muscle injury in broilers","authors":"Zhen Liu, Yingsen Liu, Tong Xing, Jiaolong Li, Lin Zhang, Liang Zhao, Yun Jiang, Feng Gao","doi":"10.1186/s40104-024-01093-6","DOIUrl":"https://doi.org/10.1186/s40104-024-01093-6","url":null,"abstract":"Chronic heat stress (CHS) is a detrimental environmental stressor with a negative impact on the meat quality of broilers. However, the underlying mechanisms are not fully understood. This study investigates the effects of CHS on long non-coding RNA (lncRNA) expression and muscle injury in broilers, with a focus on its implications for meat quality. The results showed that CHS diminished breast muscle yield, elevated abdominal fat deposition, induced cellular apoptosis (P < 0.05), and caused myofibrosis. Transcriptomic analysis revealed 151 differentially expressed (DE) lncRNAs when comparing the normal control (NC) and HS groups, 214 DE lncRNAs when comparing the HS and PF groups, and 79 DE lncRNAs when comparing the NC and pair-fed (PF) groups. After eliminating the confounding effect of feed intake, 68 lncRNAs were identified, primarily associated with cellular growth and death, signal transduction, and metabolic regulation. Notably, the apoptosis-related pathway P53, lysosomes, and the fibrosis-related gene TGF-β2 were significantly upregulated by lncRNAs. These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA, leading to connective tissue accumulation, which likely contributes to reduced breast muscle yield and meat quality in broilers.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"29 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142384217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1186/s40104-024-01094-5
Jiayu Ma, Shenfei Long, Jian Wang, Jie Gao, Xiangshu Piao
<p> <b>Correction: J Animal Sci Biotechnol 13, 16 (2022)</b> </p><p><b>https://doi.org/10.1186/s40104-021-00670-3</b> </p><br/><p>Following publication of the original article [1], the authors reported an error in the MOA product and antibiotic section under Materials and Methods.</p><p>The original texts of this section was:</p><p>The product of MOA combination named PORCINATTM was supplied by Jefo (Jefagro, Canada), which is a selected formulation of essential oils primarily containing thymol, vanillin and eugenol and organic acid mainly containing fumaric, citric, butyric and sorbic acid microencapsulated in the triglyceride matrix of hydrogenated vegetable oils. The chlortetracycline was sourced from Tongli Xingke (Beijing Tonglixingke, China).</p><p>The correct texts should be:</p><p>The product of MOA combination named PORCINAT + ™ was supplied by Jefo Nutrition Inc. (Jefagro, Saint-Hyacinthe, Canada), which is a selected formulation of essential oils primarily containing thymol, vanillin and eugenol and organic acids mainly containing fumaric, sorbic, malic and citric acid microencapsulated in a triglyceride matrix of hydrogenated vegetable oils. The chlortetracycline was sourced from Beijing Tongli Xingke Agriculture Technology Co., Ltd. (Beijing, China).</p><p>The original article [1] has been updated. </p><ol data-track-component="outbound reference" data-track-context="references section"><li data-counter="1."><p>Ma J, Long S, Wang J, et al. Microencapsulated essential oils combined with organic acids improves immune antioxidant capacity and intestinal barrier function as well as modulates the hindgut microbial community in piglets. J Animal Sci Biotechnol. 2022;13:16. https://doi.org/10.1186/s40104-021-00670-3.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-download-medium" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China</p><p>Jiayu Ma, Shenfei Long, Jian Wang, Jie Gao & Xiangshu Piao</p></li><li><p>Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China</p><p>Jie Gao</p></li></ol><span>Authors</span><ol><li><span>Jiayu Ma</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Shenfei Long</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jian Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jie Gao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></
更正：J Animal Sci Biotechnol 13, 16 (2022)https://doi.org/10.1186/s40104-021-00670-3Following 原文[1]发表后，作者报告了 "材料与方法 "中MOA产品和抗生素部分的一处错误。该部分的原文是：MOA 组合产品名为 PORCINATTM，由 Jefo 公司（加拿大 Jefagro 公司）提供，它是一种精选配方，将主要含有百里酚、香兰素和丁香酚的精油和主要含有富马酸、柠檬酸、丁酸和山梨酸的有机酸微囊化在氢化植物油的甘油三酯基质中。正确的文本应为：MOA 组合产品 PORCINAT + ™ 由 Jefo Nutrition Inc.(Jefagro, Saint-Hyacinthe, Canada）提供，它是一种精选配方，由主要含有百里酚、香兰素和丁香酚的精油和主要含有富马酸、山梨酸、苹果酸和柠檬酸的有机酸微囊化在氢化植物油的甘油三酯基质中。金霉素来自北京同力兴科农业科技有限公司（中国北京）。(Ma J, Long S, Wang J, et al. 微胶囊精油与有机酸结合可提高免疫抗氧化能力和肠道屏障功能并调节仔猪后肠微生物群落。J Animal Sci Biotechnol.2022;13:16. https://doi.org/10.1186/s40104-021-00670-3.Article CAS Google Scholar Download references作者及单位中国农业大学动物科技学院动物营养国家重点实验室，北京，100193马家玉，龙申飞，王健，高杰&；中国农业科学院动物研究所，北京，100193、中国Jie Gao作者Jiayu Ma查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Shenfei Long查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Jian Wang查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者作者简介Jie Gao查看作者发表的论文您也可以在 PubMed Google Scholar中搜索该作者Xiangshu Piao查看作者发表的论文您也可以在 PubMed Google Scholar中搜索该作者通信作者Xiangshu Piao.开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但必须注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则创作共用公共领域专用免责声明 (http://creativecommons.org/publicdomain/zero/1.0/) 适用于本文提供的数据。转载与许可引用本文Ma, J., Long, S., Wang, J. et al. Correction：微胶囊精油与有机酸结合可提高免疫抗氧化能力和肠道屏障功能，并调节仔猪后肠微生物群落。J Animal Sci Biotechnol 15, 136 (2024). https://doi.org/10.1186/s40104-024-01094-5Download citationPublished: 27 September 2024DOI: https://doi.org/10.1186/s40104-024-01094-5Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative.
{"title":"Correction: Microencapsulated essential oils combined with organic acids improves immune antioxidant capacity and intestinal barrier function as well as modulates the hindgut microbial community in piglets","authors":"Jiayu Ma, Shenfei Long, Jian Wang, Jie Gao, Xiangshu Piao","doi":"10.1186/s40104-024-01094-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01094-5","url":null,"abstract":"<p>\u0000<b>Correction: J Animal Sci Biotechnol 13, 16 (2022)</b>\u0000</p><p><b>https://doi.org/10.1186/s40104-021-00670-3</b>\u0000</p><br/><p>Following publication of the original article [1], the authors reported an error in the MOA product and antibiotic section under Materials and Methods.</p><p>The original texts of this section was:</p><p>The product of MOA combination named PORCINATTM was supplied by Jefo (Jefagro, Canada), which is a selected formulation of essential oils primarily containing thymol, vanillin and eugenol and organic acid mainly containing fumaric, citric, butyric and sorbic acid microencapsulated in the triglyceride matrix of hydrogenated vegetable oils. The chlortetracycline was sourced from Tongli Xingke (Beijing Tonglixingke, China).</p><p>The correct texts should be:</p><p>The product of MOA combination named PORCINAT + ™ was supplied by Jefo Nutrition Inc. (Jefagro, Saint-Hyacinthe, Canada), which is a selected formulation of essential oils primarily containing thymol, vanillin and eugenol and organic acids mainly containing fumaric, sorbic, malic and citric acid microencapsulated in a triglyceride matrix of hydrogenated vegetable oils. The chlortetracycline was sourced from Beijing Tongli Xingke Agriculture Technology Co., Ltd. (Beijing, China).</p><p>The original article [1] has been updated.\u0000</p><ol data-track-component=\"outbound reference\" data-track-context=\"references section\"><li data-counter=\"1.\"><p>Ma J, Long S, Wang J, et al. Microencapsulated essential oils combined with organic acids improves immune antioxidant capacity and intestinal barrier function as well as modulates the hindgut microbial community in piglets. J Animal Sci Biotechnol. 2022;13:16. https://doi.org/10.1186/s40104-021-00670-3.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China</p><p>Jiayu Ma, Shenfei Long, Jian Wang, Jie Gao & Xiangshu Piao</p></li><li><p>Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China</p><p>Jie Gao</p></li></ol><span>Authors</span><ol><li><span>Jiayu Ma</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Shenfei Long</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jian Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jie Gao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"55 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-19DOI: 10.1186/s40104-024-01079-4
Sara Virdis, Diana Luise, Federico Correa, Luca Laghi, Norma Arrigoni, Roxana Elena Amarie, Andrea Serra, Giacomo Biagi, Clara Negrini, Francesco Palumbo, Paolo Trevisi
The prolificacy of sows (litter size at birth) has markedly increased, leading to higher post-natal mortality. Heat stress can exacerbate this issue. Arginine plays an important role in several physiological pathways; its effect on gestating sows can depend on the period of supplementation. This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons, using a multi-omics approach. A total of 320 sows were divided into 4 groups over 2 seasons (warm/cold); a control group (CO) received a standard diet (including 16.5 g/d of arginine) and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine (38.3 g/d of arginine) either during the first 35 d (Early35), the last 45 d (Late45) or throughout the entire gestation period (COM). The colostrum was analyzed for nutritional composition, immunoglobulins and metabolomic profile. Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles. Piglet body weight and mortality were recorded at birth, d 6, d 26, and on d 14 post-weaning. Interactions between arginine and season were never significant. The Early35 group had a lower percentage of stillborn (P < 0.001), mummified (P = 0.002) and low birthweight (LBW) piglets (P = 0.02) than the CO group. The Late45 group had a lower percentage of stillborn piglets (P = 0.029) and a higher percentage of high birthweight piglets (HBW; P < 0.001) than the CO group. The COM group had a higher percentage of LBW (P = 0.004) and crushed piglets (P < 0.001) than the CO group. Arginine supplementation modifies the metabolome characterization of colostrum, urine, and feces. Creatine and nitric oxide pathways, as well as metabolites related to microbial activity, were influenced in all matrices. A slight trend in the beta diversity index was observed in the microbiome profile on d 35 (P = 0.064). Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets, while in the last third of pregnancy, it favored the percentage of HBW pigs and reduced the percentage of stillbirths, showing that arginine plays a significant role in the physiology of pregnant sows.
{"title":"Productive and metabolomic consequences of arginine supplementation in sows during different gestation periods in two different seasons","authors":"Sara Virdis, Diana Luise, Federico Correa, Luca Laghi, Norma Arrigoni, Roxana Elena Amarie, Andrea Serra, Giacomo Biagi, Clara Negrini, Francesco Palumbo, Paolo Trevisi","doi":"10.1186/s40104-024-01079-4","DOIUrl":"https://doi.org/10.1186/s40104-024-01079-4","url":null,"abstract":"The prolificacy of sows (litter size at birth) has markedly increased, leading to higher post-natal mortality. Heat stress can exacerbate this issue. Arginine plays an important role in several physiological pathways; its effect on gestating sows can depend on the period of supplementation. This study evaluated the effects of arginine supplementation on the productive performance and physiological status of sows during different gestation periods and seasons, using a multi-omics approach. A total of 320 sows were divided into 4 groups over 2 seasons (warm/cold); a control group (CO) received a standard diet (including 16.5 g/d of arginine) and 3 other groups received the standard diet supplemented with 21.8 g/d of arginine (38.3 g/d of arginine) either during the first 35 d (Early35), the last 45 d (Late45) or throughout the entire gestation period (COM). The colostrum was analyzed for nutritional composition, immunoglobulins and metabolomic profile. Urine and feces were analyzed on d 35 and 106 for the metabolomic and microbial profiles. Piglet body weight and mortality were recorded at birth, d 6, d 26, and on d 14 post-weaning. Interactions between arginine and season were never significant. The Early35 group had a lower percentage of stillborn (P < 0.001), mummified (P = 0.002) and low birthweight (LBW) piglets (P = 0.02) than the CO group. The Late45 group had a lower percentage of stillborn piglets (P = 0.029) and a higher percentage of high birthweight piglets (HBW; P < 0.001) than the CO group. The COM group had a higher percentage of LBW (P = 0.004) and crushed piglets (P < 0.001) than the CO group. Arginine supplementation modifies the metabolome characterization of colostrum, urine, and feces. Creatine and nitric oxide pathways, as well as metabolites related to microbial activity, were influenced in all matrices. A slight trend in the beta diversity index was observed in the microbiome profile on d 35 (P = 0.064). Arginine supplementation during early gestation reduced the percentage of stillborn and LBW piglets, while in the last third of pregnancy, it favored the percentage of HBW pigs and reduced the percentage of stillbirths, showing that arginine plays a significant role in the physiology of pregnant sows.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1186/s40104-024-01083-8
Fernando H. Biase, Sarah E. Moorey, Julie G. Schnuelle, Soren Rodning, Martha Sofia Ortega, Thomas E. Spencer
MicroRNAs (miRNAs) are presented in the uterine lumen of many mammals, and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions. Our aim was to identify and contrast the miRNAs present in extracellular vesicles (EVs) in the uterine lumen fluid (ULF) at the onset of attachment in cattle pregnancies (gestation d 18) initiated by artificial insemination (AI) or by the transfer of an in vitro-produced blastocyst (IVP-ET). A third group had no conceptus after the transfer of an IVP embryo. The abundance of 263 annotated miRNAs was quantified in the EVs collected from ULF. There was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group, while 4 miRNAs had a lower abundance relative to the group not containing a conceptus. Additionally, 4 miRNAs were more abundant in ULF EVs in the AI pregnant group relative to IVP-ET group (bta-mir-17, bta-mir-7-3, MIR7-1, MIR18A). Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endometrium, including genes that are known to be their targets. The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation, as well as in endometrium receptivity. The knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of corrective approaches to reduce conceptus losses during the first month of pregnancy in cattle.
{"title":"Altered microRNA composition in the uterine lumen fluid in cattle (Bos taurus) pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo","authors":"Fernando H. Biase, Sarah E. Moorey, Julie G. Schnuelle, Soren Rodning, Martha Sofia Ortega, Thomas E. Spencer","doi":"10.1186/s40104-024-01083-8","DOIUrl":"https://doi.org/10.1186/s40104-024-01083-8","url":null,"abstract":"MicroRNAs (miRNAs) are presented in the uterine lumen of many mammals, and in vitro experiments have determined that several miRNAs are important for the regulation of endometrial and trophoblast functions. Our aim was to identify and contrast the miRNAs present in extracellular vesicles (EVs) in the uterine lumen fluid (ULF) at the onset of attachment in cattle pregnancies (gestation d 18) initiated by artificial insemination (AI) or by the transfer of an in vitro-produced blastocyst (IVP-ET). A third group had no conceptus after the transfer of an IVP embryo. The abundance of 263 annotated miRNAs was quantified in the EVs collected from ULF. There was an increase in the transcript abundance of 20 miRNAs in the ULF EVs from the AI pregnant group, while 4 miRNAs had a lower abundance relative to the group not containing a conceptus. Additionally, 4 miRNAs were more abundant in ULF EVs in the AI pregnant group relative to IVP-ET group (bta-mir-17, bta-mir-7-3, MIR7-1, MIR18A). Specific miRNAs in the ULF EVs were co-expressed with messenger RNAs expressed in extra-embryonic tissues and endometrium, including genes that are known to be their targets. The results provide biological insights into the participation of miRNAs in the regulation of trophoblast proliferation and differentiation, as well as in endometrium receptivity. The knowledge that in vitro cultured embryos can contribute to the altered abundance of specific miRNAs in the uterine lumen can lead to the development of corrective approaches to reduce conceptus losses during the first month of pregnancy in cattle.\u0000","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"386 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142174692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Addressing the shortage of high-quality protein resources, this study was conducted to investigate the effects of replacing soybean meal (SBM) with different levels of enzymolysis-fermentation compound protein feed (EFCP) in the diets of growing-finishing pigs, focusing on growth performance, nutrients digestibility, carcass traits, and meat quality. Sixty DLY (Duroc × Landrace × Yorkshire) pigs with an initial body weight of 42.76 ± 2.05 kg were assigned to 5 dietary treatments in a 2 × 2 + 1 factorial design. These dietary treatments included a corn-soybean meal diet (CON), untreated compound protein feed (UCP) substitution 50% (U50) and 100% SBM (U100) diets, and EFCP substitution 50% (EF50) and 100% SBM (EF100) diets. Each treatment had 6 pens (replicates) with 2 pigs per pen, and the experiment lasted 58 d, divided into phase I (1–28 d) and phase II (29–58 d). Following phase I, only the CON, U50, and EF50 groups were continued for phase II, each with 5 replicate pens. On d 59, a total of 15 pigs (1 pig/pen, 5 pens/treatment) were euthanized. During phase I, the EF50 group had a higher average daily gain (ADG) in pigs (P < 0.05) compared to the CON group, whereas the U50 group did not have a significant difference. As the substitution ratio of UCP and EFCP increased in phase I, there was a noticeable reduction in the final body weight and ADG (P < 0.05), along with an increase in the feed-to-gain ratio (F/G) (P < 0.05). In phase II, there were no significant differences in growth performance among the treatment groups, but EF50 increased the apparent digestibility of several nutrients (including dry matter, crude protein, crude fiber, acid detergent fiber, ash, gross energy) compared to U50. The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups (P < 0.05). Moreover, the EF50 group had higher carcass weight and carcass length than those in the CON and U50 groups (P < 0.05), with no significant difference in meat quality. The study findings suggest that replacing 50% SBM with EFCP during the growing-finishing period can improve the growth performance, nutrient digestibility, and carcass traits of pigs without compromising meat quality. This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.
{"title":"Effects of replacing soybean meal with enzymolysis-fermentation compound protein feed on growth performance, apparent digestibility of nutrients, carcass traits, and meat quality in growing-finishing pigs","authors":"Yu Cheng, Jun He, Ping Zheng, Jie Yu, Junning Pu, Zhiqing Huang, Xiangbing Mao, Yuheng Luo, Junqiu Luo, Hui Yan, Aimin Wu, Bing Yu, Daiwen Chen","doi":"10.1186/s40104-024-01080-x","DOIUrl":"https://doi.org/10.1186/s40104-024-01080-x","url":null,"abstract":"Addressing the shortage of high-quality protein resources, this study was conducted to investigate the effects of replacing soybean meal (SBM) with different levels of enzymolysis-fermentation compound protein feed (EFCP) in the diets of growing-finishing pigs, focusing on growth performance, nutrients digestibility, carcass traits, and meat quality. Sixty DLY (Duroc × Landrace × Yorkshire) pigs with an initial body weight of 42.76 ± 2.05 kg were assigned to 5 dietary treatments in a 2 × 2 + 1 factorial design. These dietary treatments included a corn-soybean meal diet (CON), untreated compound protein feed (UCP) substitution 50% (U50) and 100% SBM (U100) diets, and EFCP substitution 50% (EF50) and 100% SBM (EF100) diets. Each treatment had 6 pens (replicates) with 2 pigs per pen, and the experiment lasted 58 d, divided into phase I (1–28 d) and phase II (29–58 d). Following phase I, only the CON, U50, and EF50 groups were continued for phase II, each with 5 replicate pens. On d 59, a total of 15 pigs (1 pig/pen, 5 pens/treatment) were euthanized. During phase I, the EF50 group had a higher average daily gain (ADG) in pigs (P < 0.05) compared to the CON group, whereas the U50 group did not have a significant difference. As the substitution ratio of UCP and EFCP increased in phase I, there was a noticeable reduction in the final body weight and ADG (P < 0.05), along with an increase in the feed-to-gain ratio (F/G) (P < 0.05). In phase II, there were no significant differences in growth performance among the treatment groups, but EF50 increased the apparent digestibility of several nutrients (including dry matter, crude protein, crude fiber, acid detergent fiber, ash, gross energy) compared to U50. The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups (P < 0.05). Moreover, the EF50 group had higher carcass weight and carcass length than those in the CON and U50 groups (P < 0.05), with no significant difference in meat quality. The study findings suggest that replacing 50% SBM with EFCP during the growing-finishing period can improve the growth performance, nutrient digestibility, and carcass traits of pigs without compromising meat quality. This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142170888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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