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Fungal pathogens of mango (Mangifera indica L.) inflorescences 芒果(Mangifera indica L.)花序真菌病原菌
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-04 DOI: 10.46429/JAUPR.V104I2.19039
L. Rivera-Vargas, Manuel Pérez-Cuevas, Irma Cabrera-Asencio, M. R. Suárez-Rozo, Luz M. Serrato-Díaz
This is the first comprehensive study to identify fungal pathogens of mango (Mangifera indica L.) inflorescences in Puerto Rico. A total of 452 mango inflorescences were collected from four cultivars at seven developmental stages during two blooming seasons. Samples were gathered from the germplasm collection at the Agricultural Experiment Station of the University of Puerto Rico in Juana Díaz, Puerto Rico. Eight different symptoms were observed: cankers, flower abortion, powdery mildew, rachis necrotic lesions, rachis soft rot, tip blight, vascular wilt, and insect perforations with necrotic borders. Necrosis was the most prevalent symptom (47%), followed by powdery mildew (19%) and tip blight (6%). Symptoms of malformation were never observed in the field. Using a modified Horsfall and Barratt scale, data on all mango cultivars pooled from two blooming seasons showed that the full bloom stage, the last inflorescence developmental stage (G), displayed the highest mean disease severity (42.67%). This severity value was significantly higher than those of the other developmental stages evaluated (P<0.05). Early inflorescence developmental stages were asymptomatic or showed the lowest percentage of disease severity. An ANOVA was performed to compare disease severity among all mango cultivars regardless of developmental stage. Results showed that there were significant differences (P<0.05) between mean disease severity of cultivars ‘Parvin’ and ‘Haden’. Mean disease severity was higher in ‘Haden’ (20%) when compared to ‘Parvin’ (10.7%). There were no statistical differences in mean disease severity between cultivars ‘Irwin’, ‘Keitt’ and ‘Parvin’, or between ‘Irwin’, ‘Haden’ and ‘Keitt’. In addition to the powdery mildew caused by Pseudoidium anacardii, 26 genera of fungi, mainly of Ascomycetes, 1Manuscript submitted to Editorial Board 18 June 2019. 2This work was supported by the USDA National Institute of Food and Agriculture, Hatch project H-425. The authors thank agronomists Víctor M. González, Luis E. Collazo and Lorena L. Simbaña for their assistance in the development of this research. 3Professor, Department of Agro-environmental Sciences, University of Puerto RicoMayagüez, Mayagüez, P.R. 00680; e-mail: lydiai.rivera@upr.edu. 4Ex-graduate student, Department of Agro-environmental Sciences, University of Puerto Rico-Mayagüez. 5Professor, Department of Agro-environmental Sciences, University of Puerto Rico, Agricultural Experiment Station, Juana Díaz, P.R. 0079. 6Research Associate, Department of Agro-environmental Sciences, University of Puerto Rico-Mayagüez. 7Research Plant Pathologist, USDA-ARS-Tropical Agriculture Research Station, 2200 P.A. Campos Avenue Ste. 201, Mayagüez, Puerto Rico 00680-5470. 140 RiveRa-vaRgas et al./Fungi oF Mango inFloRescences were identified from a total of 569 fungal isolates, from symptomatic and asymptomatic inflorescences. The most common fungi were: Diaporthe spp. (29%), followed by members of the Botryosphaeri
这是首次在波多黎各鉴定芒果(Mangifera indica L.)花序真菌病原体的综合研究。在两个开花季节,收集了4个品种7个发育阶段的452个芒果花序。样本从波多黎各胡安娜Díaz的波多黎各大学农业实验站的种质收集中收集。观察到8种不同的症状:溃疡病、花流产、白粉病、轴坏死性病变、轴软腐病、尖端枯萎病、血管枯萎病和带坏死边界的昆虫穿孔。坏死是最常见的症状(47%),其次是白粉病(19%)和叶枯病(6%)。在现场从未观察到畸形症状。利用改良的Horsfall和Barratt量表,收集了两个开花季节的所有芒果品种的数据,结果表明,盛开期(花序发育的最后阶段)的平均疾病严重程度最高(42.67%)。该严重程度值显著高于其他发育阶段(P<0.05)。早期花序发育阶段无症状或疾病严重程度百分比最低。采用方差分析比较不同发育阶段所有芒果品种的疾病严重程度。结果表明,‘Parvin’和‘Haden’品种的平均病害严重程度差异显著(P<0.05)。“Haden”的平均疾病严重程度(20%)高于“Parvin”(10.7%)。栽培品种' Irwin '、' Keitt '和' Parvin '之间,或' Irwin '、' Haden '和' Keitt '之间的平均疾病严重程度无统计学差异。除由假丝胞菌引起的白粉病外,还有26属真菌,主要是子囊菌,1稿提交编委会2019年6月18日。这项工作得到了美国农业部国家食品和农业研究所H-425项目的支持。作者感谢农学家Víctor M. González、Luis E. Collazo和Lorena L. Simbaña对这项研究发展的帮助。3波多黎各大学农业环境科学系教授,波多黎各玛雅 ez,波多黎各00680;电子邮件:lydiai.rivera@upr.edu。4波多黎各大学农业环境科学系研究生。5波多黎各大学农业环境科学系教授,农业实验站,胡安娜Díaz, P.R. 0079。6波多黎各大学农业环境科学系研究助理,波多黎各;7美国农业部热带农业研究站植物病理学研究中心,波多黎各马亚 ez市坎波斯大道201街2200号,00680-5470。140 RiveRa-vaRgas等人/芒果花序真菌从有症状和无症状花序中共分离出569株真菌。最常见的真菌是:Diaporthe spp.(29%),其次是Botryosphaeriaceae成员(16%),曲霉属(11%)和镰刀菌属(11%)。本研究中发现的许多真菌病原体都是从无症状组织中分离出来的,以内生菌或潜伏病原体的形式出现:A. alternata, botryosphaeriiaceae的各种成员,C. gloeosporioides复合体,Cladosporium spp.和F. decemcellulare。因此,使用保护性杀菌剂在控制真菌方面不如使用系统性杀菌剂有效。在实施检疫规定时,正确鉴定影响芒果花序的真菌病原体非常重要。此外,这些信息将有助于制定更好的芒果果园管理战略。
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引用次数: 0
Willingness to pay for the conservation of an urban forest at the Montaña farm, Aguadilla, Puerto Rico 愿意为波多黎各阿瓜迪拉Montaña农场的城市森林保护付费
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-02 DOI: 10.46429/JAUPR.V104I2.19037
Kenneth A. Rivera-Acosta
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引用次数: 0
Feeding systems and productive and physiological parameters of lambs raised under heat stress 热应激下饲养羔羊的饲养系统和生产生理参数
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-02 DOI: 10.46429/JAUPR.V104I2.19041
A. Rodríguez-Carías, Jose Israel Suarez-Rodriguez, Jonathan Collazo, J. F. Cleve
The objective of this study was to determine physiological parameters (rectal temperature = RT, respiratory rate = RR, heart rate = HR) and production parameters (dry matter intake = DMI, water intake = WI, average daily weight gain = ADG, and feed conversion = FC) of crossbred and Katahdin lambs raised in rotational grazing of native pastures (NP) and in confinement under heat stress conditions. Nine crossbred lambs (average initial weight = 19.4 kg) were used and fed in a rotational grazing system for 70 days with occupation and rest periods of seven and 21 days, respectively. The lambs had daily access to the grazing area for four to six hours, grass hay and water ad libitum, a daily supplement of 100 g of commercial concentrate and a nutritional block. In the confined system, six lambs of the Katahdin breed (average initial weight = 27.3 kg) were used, and for 28 days these were fed total mixed rations (TMR) containing 30% grass hay, 15.4% soybean meal, 54.6% corn grain and a mineral block. The diet contained 14% Crude Protein (CP) and 66% Total Digestible Nutrients (TDN) and was formulated for an ADG of 200 g. The offered and rejected feed was quantified every day [4% body weight (BW)/dry matter basis (DMB)] to determine the DMI, and the volume of water offered and rejected to determine WI. To validate the stress conditions, the maximum environmental temperature and relative humidity were monitored to calculate the temperature and humidity index (THI), and the RT, HR and RR of the 15 lambs were determined. The lambs were weighed every seven days. In both experiments, the THI oscillated between 78 and 80. The lambs were under environmental heat stress conditions (THI x̅ = 79) during both studies. The averages of HR and RT in the grazing system were 118.0 beats per minute and 38.6° C, while the averages under the confinement system were 108.6 beats per minute and 39.4° C, respectively. The average RR (53.6 and 62.6 breaths per minute, in grazing and in confinement, respectively) was greater than the normal value considered for thermal comfort. Respiratory rate is the main mechanism used by lambs to dissipate heat. The heart rate also showed slightly higher values than those reported for animals in their comfort zone. However, the rectal temperature values were constant. As expected, crossbred lambs fed under grazing conditions with naturalized pastures (NP) had a low ADG (55.6 g). Nevertheless, Katahdin lambs fed in confinement with TMR had a DMI of 935.4 g/day (3.14% BW/DMB), a WI of 3.5 L/day, a FC of 4.74 and an ADG of 226.2 g. In conclusion, raising crossbred sheep in rotational grazing of NP without any type of agronomic management results in ADG that could not be considered viable in traditional production systems. The introduction of pure breeds and feeding them in confined systems with a TMR results in a pre-determined ADG (>200 g/day) and is an alternative that can be evaluated economically for its practical implementation.
本研究的目的是确定热应激条件下轮牧和圈养条件下杂交羔羊和卡塔丁羔羊的生理参数(直肠温度= RT、呼吸频率= RR、心率= HR)和生产参数(干物质采食量= DMI、水分采食量= WI、平均日增重= ADG和饲料系数= FC)。选用9只平均初重19.4 kg的杂交羔羊,采用轮牧方式饲养70 d,占用期7 d,休息期21 d。这些羔羊每天可在放牧区活动4至6小时,可随意使用干草和水,每天补充100克商业浓缩物和营养块。在密闭饲养系统中,选用6只平均初始体重为27.3 kg的卡塔丁(Katahdin)羔羊,饲喂含30%干草、15.4%豆粕、54.6%玉米和矿物块的全混合口粮(TMR) 28 d。饲粮粗蛋白质(CP)含量为14%,总可消化营养物质(TDN)含量为66%,平均日增重为200 g。每天定量饲喂和拒收饲料[4%体重/干物质基础(DMB)]以确定DMI,定量饲喂和拒收水量以确定WI。为了验证应激条件,监测最高环境温度和相对湿度,计算温湿度指数(THI),并测定15只羔羊的RT、HR和RR。羊羔每七天称一次体重。在两个实验中,THI在78和80之间振荡。在两项研究中,羔羊都处于环境热应激条件下(THI x′s = 79)。放牧条件下的HR和RT平均值分别为118.0次/ min和38.6°C,封闭条件下的HR和RT平均值分别为108.6次/ min和39.4°C。平均呼吸比(放牧和禁闭时分别为53.6次和62.6次/分钟)大于热舒适的正常值。呼吸速率是羔羊散热的主要机制。心率也比在舒适区的动物略高。然而,直肠温度值是恒定的。正如预期的那样,在归化牧场(NP)放牧条件下饲养的杂交羔羊的平均日增重较低(55.6 g),而在TMR条件下饲养的Katahdin羔羊的平均日增重为935.4 g/d (3.14% BW/DMB), WI为3.5 L/d, FC为4.74,平均日增重为226.2 g。综上所述,在没有任何农艺管理的情况下,在NP轮牧条件下饲养杂交绵羊,其平均日增重在传统生产系统中是不可行的。引进纯品种并在具有TMR的密闭系统中饲养,可获得预先确定的日增重(100 - 200克/天),是一种可在实际实施中进行经济评估的替代方案。
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引用次数: 0
Weight gain, carcass characteristics, and meat quality of native lambs fed in confinement with total rations 全日粮月龄本地羔羊的增重、胴体特性和肉质
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-02 DOI: 10.46429/JAUPR.V104I2.19042
J. J. Rosado, A. Rodríguez-Carías, Aixa Rivera, J. F. Cleve
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引用次数: 0
Título en inglés 英文标题
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-02 DOI: 10.46429/JAUPR.V104I2.19044
Wilfredo Robles, María de L. Lugo-Torres, J. P. Morales-Payán
The central giant cell granuloma is an intraosseous, osteolytic and benign lesion of non-odontogenic origin, with no reports published so far that can become a malignant pathology. We presented a 69-years-old male who presented intraoral volume increase on the left side, evolution time was not quantified, non-painful, prevented the placement of his dental prosthesis. Image studies and stereolithographic models were performed and demonstrate the presence of a wide lithic zone located below the piriform aperture. Cystic mass enucleation, aggressive curettage of bony walls and cleaning of surgical field with chemical solution was done. Our objective is to provide necessary guidelines for central giant cell granuloma diagnosis in order to carry out an adequate treatment without risk of recurrence.
中央巨细胞肉芽肿是一种非牙源性的骨内溶骨性良性病变,迄今为止尚未发表可成为恶性病理的报告。我们介绍了一名69岁的男性,他左侧口腔内容积增加,进化时间没有量化,没有疼痛,无法放置假牙。进行了图像研究和立体光刻模型,证明梨状孔下方存在一个宽阔的石器地带。进行囊性肿块摘除术、骨壁刮除术和化学溶液清洁手术区域。我们的目的是为中央巨细胞肉芽肿的诊断提供必要的指导,以便在没有复发风险的情况下进行充分的治疗。
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引用次数: 0
Identifying interspecific lines of coffee resistant to Hemileia vastatrix Berk. et Br. and evaluating their adaptability to the coffee growing region of Puerto Rico 咖啡抗半黑叶螨种间系的鉴定。Br。并评估它们对波多黎各咖啡种植区的适应性
Q4 Agricultural and Biological Sciences Pub Date : 2021-06-02 DOI: 10.46429/JAUPR.V104I2.19040
R. Rodríguez, Wigmar González, Oswaldo Bosques
Thirteen lines from the coffee germplasm collection of the Adjuntas Agricultural Experiment Substation, lines T-5175 and T-8667 from Costa Rica, and four advanced multilines from the Portugal Research Center for Coffee Rust (CIFC; ‘Centro de Investigación de la Roya del Café de Portugal’) were evaluated for resistance to Hemileia vastatrix and their performance under coffee production conditions in Puerto Rico. The evaluations were conducted in the laboratory and on selected coffee farms. Lines of “Catimor” and “Sarchimor” types were selected based on their reaction to the rust pathogen and excellent agronomic characteristics and named ‘Frontón’ and ‘Limaní’, respectively. Incidence of black beans was examined in red, medium and green beans and was significantly higher in line T-5175, in plantings <300 meters above sea level, and in medium ripe and green fruits. Catimor lines from the CIFC were superior to the check ‘Caturra’ in fruit production and yield of green coffee. 
13个品系来自Adjuntas农业试验变电站的咖啡种质收集,T-5175和T-8667品系来自哥斯达黎加,以及4个来自葡萄牙咖啡锈病研究中心的先进多品系(CIFC;“Centro de Investigación de la Roya del cafede Portugal”)在波多黎各的咖啡生产条件下,评估了它们对黑蚜的抗性及其表现。评估是在实验室和选定的咖啡农场进行的。根据“Catimor”和“Sarchimor”对锈病病原的反应和优良的农艺性状选择品系,分别命名为“Frontón”和“Limaní”。黑豆的发病率在红豆、中豆和青豆中进行了检测,在T-5175品系、海拔<300米的种植、中熟果和青果中显著高于黑豆。在果产量和生咖啡产量方面,来自CIFC的Catimor品系优于对照‘Caturra’。
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引用次数: 0
Effect of sex and animal type on tenderness and juiciness of beef from cattle with eight permanent incisors in Puerto Rico 性别和动物类型对波多黎各有8个永久门牙的牛肉质嫩度和多汁性的影响
Q4 Agricultural and Biological Sciences Pub Date : 2021-04-05 DOI: 10.46429/JAUPR.V101I1.14293
K. Domenech, Aixa Rivera, Américo Casas, M. Pagán, D. Cianzio, F. Pérez
The effects of sex (female or intact male) and animal type (beef, BT; or dairy, DT) on meat quality attributes of the Longissimus lumborum muscle were evaluated in 89 commercially sourced bovines in Puerto Rico, all having eight permanent incisors (>53 months of age). Mean meat pH was greater (P<0.0001) for BT than for DT (5.75 vs. 5.40), with males of BT and DT having the highest and lowest values, respectively (5.99 vs. 5.32), resulting in a sex x type interaction (P=0.0003). Regarding color parameters, female BT and male DT had lower L* values (darker meat), constituting another sex x type interaction (P<0.0001). Males showed greater a* and b* values than females (more red and yellow colored meat; P<0.05). Females surpassed males (P<0.0001) in intramuscular fat content (9.13 vs. 3.06%) and DT showed a non-significant advantage over BT (8.90 vs. 5.87%). Higher pH was associated with greater water retention (P<0.0001). Warner-Bratzler Shear (WBS) and tenderness scores assigned by untrained sensory panelists indicated non-significant tendencies in favor of females (WBS: 6.13 vs. 7.20 kg), and in favor of BT over DT within males (WBS: 6.69 vs. 7.71 kg). The sensory panel assigned higher juiciness scores to beef from males than females (4.85 vs. 4.16 on a scale from 1 to 8; P=0.0074).
性别(雌性或完整雄性)和动物类型(牛肉、BT;对波多黎各89头商业来源的牛(均有8个恒门牙(>53月龄))的腰最长肌肉质属性进行了评估。肉质平均pH值BT比DT(5.75比5.40)大(P<0.0001),其中BT和DT雄性分别最高和最低(5.99比5.32),导致性别x型相互作用(P=0.0003)。在颜色参数方面,女性BT和男性DT的L*值较低(肉色较深),构成另一种性别x型相互作用(P<0.0001)。雄性的a*和b*值高于雌性(红色和黄色的肉较多;P < 0.05)。女性肌内脂肪含量高于男性(P<0.0001)(9.13比3.06%),DT比BT无显著优势(8.90比5.87%)。较高的pH值与较高的水潴留相关(P<0.0001)。由未受过训练的感官小组成员分配的Warner-Bratzler Shear (WBS)和压痛评分显示,女性的倾向不显著(WBS: 6.13 vs. 7.20 kg),男性的BT优于DT (WBS: 6.69 vs. 7.71 kg)。感官小组给男性牛肉的多汁性评分高于女性(4.85比4.16,评分范围从1到8;P = 0.0074)。
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引用次数: 0
Comparison of body dimensions between normal and double muscled Senepol heifers 正常和双肌塞内波尔小母牛体型的比较
Q4 Agricultural and Biological Sciences Pub Date : 2021-04-05 DOI: 10.46429/JAUPR.V101I1.14291
H. Sánchez, K. Domenech, Gerardo Rivera, M. Pagán, Américo Casas, P. Randel, Gladycia Muñiz
Different body dimensions were compared between 16 normally muscled (NM) and 16 heterocygous double muscled (DM) Senepol heifers. Body weight (BW), withers height (WH), hip height (HH), thorarcic perimeter (TP), barrel (BA), shoulder to pin bone distance (SPD), and the internal pelvic width (PW), height (PH), and area (PA) were recorded in each heifer. Also included were the relationships of BW/body dimensions, except for dimensions PW and PH. Data were analyzed by the GLIMMIX and CORR procedures of SAS. The respective genotypes MN and DM did not differ (P>0.10) in BW (415 and 434 kg), WH (both 113 cm), HH (both 120 cm), TP (both 175 cm), B A (209 and 214 cm), B W/B A (1.99 and 2.03 kg/cm), SPD (both 147 cm), PW (both 13 cm), PA (212 and 220 cm2), and BW/PA (1.96 and 1.98 kg/cm2). However, DM heifers tended to present (P<0.10) higher values than NM heifers for BW/WH, BW/HH, BW/SPD, and PH by margins of 0.14 kg/cm, 0.15 kg/cm, 0.12 kg/cm, and 0.59 cm, respectively. Moreover, the BW/TP value was 0.09 kg/cm greater (P<0.05) in the DM than in NM heifers. The correlations between different pairs of body dimensions were lower in the DM than in the NM heifers. These results suggest that the enhanced musculature visually observed in DM heifers must be accompanied by hypotrophy of other body organs and tissues, but not including the pelvic region. This compensatory effect minimizes the posibility of observing differences in body dimensions in vivo between DM and NM heifers.
比较了16头正常肌型(NM)和16头杂合双肌型(DM)塞内波尔母牛的体尺差异。记录每头母牛的体重(BW)、肩高(WH)、臀高(HH)、胸围(TP)、胸围(BA)、肩钉骨距离(SPD)、骨盆内宽(PW)、高度(PH)和面积(PA)。除PW和ph外,还包括体重/身体尺寸的关系。数据通过SAS的GLIMMIX和CORR程序进行分析。基因型MN和DM在体重(415和434 kg)、WH(均为113 cm)、HH(均为120 cm)、TP(均为175 cm)、B A(209和214 cm)、BW/ B A(1.99和2.03 kg/cm)、SPD(均为147 cm)、PW(均为13 cm)、PA(212和220 cm)和BW/PA(1.96和1.98 kg/cm)方面均无显著差异(P < 0.05)。体重/WH、体重/HH、体重/SPD和PH值均显著高于普通母牛(P<0.10),分别高出0.14 kg/cm、0.15 kg/cm、0.12 kg/cm和0.59 cm。DM犊牛的体重/TP值比NM犊牛高0.09 kg/cm (P<0.05)。不同对体尺之间的相关性在DM母牛中低于NM母牛。这些结果表明,在DM小母牛中,视觉上观察到的肌肉组织增强必然伴随着其他身体器官和组织的萎缩,但不包括骨盆区域。这种代偿效应最大限度地降低了观察DM和NM母牛体内体型差异的可能性。
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引用次数: 0
Caloric contribution of imported and locally produced food in Puerto Rico 波多黎各进口食品和当地生产食品的热量贡献
Q4 Agricultural and Biological Sciences Pub Date : 2021-04-05 DOI: 10.46429/JAUPR.V101I1.14298
J. Hernández, Myrna Comas-Pagán, Alwin Jiménez, S. Blas
The aim of this article is to evaluate the caloric contribution of the top imported and locally produced foods in Puerto Rico. Data from the USDA and Puerto Rico Planning Board were used to estimate caloric needs in different demographic groups and projected until 2025. In analyzing Puerto Rican produce, the caloric contribution of local production was estimated to be around 18 percent. By contrast, when the caloric contribution of 50 foods imported and produced in Puerto Rico was evaluated, these foods account for 115 percent of the calories needed daily for the population to maintain a normal weight. Sugar, rice, vegetable oil, milk and corn make the greatest contribution to the Puerto Rican diet, representing an estimated 58 percent of average number of calories that Puerto Ricans should consume. The distribution of calories identified in these 50 articles consumed in Puerto Rico (e.g., fruits, vegetables, dairy products, proteins, carbohydrates, etc.) shows serious discrepancies from the distribution suggested by the 2015-2020 Dietary Guidelines developed by experts of the U.S. Department of Health and Human Services and the U.S. Department of Agriculture. For example, 34 percent of the calories consumed in Puerto Rico correspond to foods high in sugar and fat.
本文的目的是评估波多黎各进口和当地生产的顶级食品的热量贡献。美国农业部和波多黎各规划委员会的数据被用来估计不同人口群体的热量需求,并预测到2025年。在分析波多黎各农产品时,当地生产的热量贡献估计约为18%。相比之下,当评估波多黎各进口和生产的50种食物的热量贡献时,这些食物占人口维持正常体重每天所需热量的115%。糖、大米、植物油、牛奶和玉米对波多黎各人的饮食贡献最大,估计占波多黎各人应消耗的平均卡路里数的58%。在波多黎各消费的这50种物品(如水果、蔬菜、乳制品、蛋白质、碳水化合物等)中确定的卡路里分布与美国卫生与公众服务部和美国农业部专家制定的2015-2020年膳食指南所建议的分布存在严重差异。例如,波多黎各人消耗的卡路里中有34%来自高糖和高脂肪的食物。
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引用次数: 1
Evaluation of a-amylase and protease on diet nutrient intake and digestibility, and physiological parameters in lambs a-淀粉酶和蛋白酶对羔羊饲粮养分摄入、消化率及生理参数的影响
Q4 Agricultural and Biological Sciences Pub Date : 2021-04-05 DOI: 10.46429/JAUPR.V101I1.14294
A. Rodríguez-Carías, Beatriz A. Quintana, L. C. Solórzano, P. Randel
The effects of adding exogenous enzymes on the intake and digestibility of dry matter (DM) and on nutrients, and several blood parameters were determined in lambs fed a basal diet of 34% ground corn, 40% tropical grass hay, and 26% soybean meal providing 21% dietary starch. Twelve crossbred lambs (22 kg) were assigned to one of four diets: no additives (1); diets containing α-amylase (2); protease (3); or their combination (4). Diets (DM basis) were offered daily at 4% of animal BW in four 28-day experimental periods each consisting of 21 d of adaptation to the diet followed by 7 d of complete fecal collection. In each period, feed offered, orts and feces were collected, quantified and analyzed for contents of DM, starch, crude protein (CP) and neutral detergent fiber (NDF) to determine intake and digestibility. At the end of each experimental period, blood samples were collected from the individual lambs to determine the concentration of glucose, beta-hidroxy butyrate (BHB), nonesterified fatty acids (NEFA), and insulin. Data were analyzed according to a 4 x 4 Latin Square experimental design. Dietary treatment contrasts were performed using least square means adjustment for multiple comparisons (Tukey-Kramer) as follows: enzymes versus no enzymes, amylase versus no amylase and protease versus no protease. Dry matter intake was similar across treatments (1,106; 1,088; 1,105 and 1,088 g/d for control, and diets containing α-amylase, experimental protease or their combination, respectively). Adding protease to the diet decreased (P<0.05) starch consumption (248 vs. 255 g/d) and increased, but not significantly, NDF digestibility (48.6 vs. 46.8%) as compared to that of lambs fed without the experimental enzyme. Adding enzymes to the diet tended (P<0.10) to decrease blood BHB concentration below the control level (4.26 vs. 4.68 mg/dL). Blood NEFA concentration tended to increase (P<0.10) in lambs fed α-amylase compared to those fed without α-amylase (0.17 vs. 0.14 mEq/L). The insulin level tended (P<0.10) to increase in lambs fed protease versus lambs not receiving the enzyme (80.3 vs. 71.8 pmol/L). Glucose levels were similar for all treatments. Both exogenous enzymes influenced blood metabolites; however, a greater effect was observed in lambs fed the protease.
在饲粮中添加34%的玉米粉、40%的热带干草和26%的豆粕(饲粮淀粉含量为21%),研究添加外源酶对羔羊干物质采食量和消化率、营养物质和血液指标的影响。12只杂交羔羊(22公斤)被分配到四种饲粮中的一种:不添加添加剂(1);饲粮中添加α-淀粉酶(2);蛋白酶(3);或两者的组合(4)。在4个为期28天的试验期中,以动物体重的4%饲喂日粮(以DM为基础),每个试验期21 d为日粮适应期,7 d为完全粪便收集期。每期收集所供饲料、粪便和粪便,定量分析DM、淀粉、粗蛋白质(CP)和中性洗涤纤维(NDF)含量,确定采食量和消化率。在每个实验期结束时,采集羔羊的血液样本,测定葡萄糖、丁酸乙氧基(BHB)、非酯化脂肪酸(NEFA)和胰岛素的浓度。数据分析采用4 × 4拉丁方实验设计。采用多重比较的最小二乘均数调整(Tukey-Kramer)进行膳食处理对比,如下:酶与无酶、淀粉酶与无淀粉酶、蛋白酶与无蛋白酶。各处理的干物质采食量相似(1106;1088;对照为1105和1088 g/d, α-淀粉酶、试验蛋白酶或两者组合为对照)。与不添加蛋白酶的羔羊相比,饲粮中添加蛋白酶降低了(P<0.05)淀粉消耗量(248比255 g/d),提高了NDF消化率(48.6%比46.8%),但不显著。饲粮中添加酶可使血BHB浓度(4.26 vs 4.68 mg/dL)降至对照水平以下(P<0.10)。α-淀粉酶组羔羊血NEFA浓度有升高趋势(P<0.10) (0.17 mEq/L vs. 0.14 mEq/L)。与未饲喂蛋白酶的羔羊相比,饲喂蛋白酶的羔羊胰岛素水平有升高趋势(P<0.10) (80.3 pmol/L比71.8 pmol/L)。所有治疗组的血糖水平相似。两种外源性酶都影响血液代谢物;然而,在饲喂蛋白酶的羔羊中观察到更大的效果。
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Journal of Agriculture of The University of Puerto Rico
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