Pub Date : 2017-10-28DOI: 10.17265/2161-6256/2017.10.008S
N. Khánh, N. Diem, L. Nhan, Phan Văn Cư, Trần Quang Khánh Vân, Ngô Thị Hoản
The main objective of the study was to determine the effects of nutritional media and initial cell density on the growth and development of Spirulina platensis. This study was carried out at the Center for Incubation and Technology Transfer, Hue University from May 2015 to August 2016. In the first experiment, S. platensis was cultured in four different nutritional media, including Zarrouk, Spi-RIA3, Nisole and Thuoc medium (Vietnamese). Resulted showed that S. platensis grew significantly in the Zarrouk medium rather than in other nutritional medium at the same time, and gained the maximum density of 2,736.5 mg/L after 13 d inoculums, with specific growth rate of 4.987% per day and the time to doubling population of 0.139 d. In the second experiment, S. platensis was cultured in six different initial cell densities: 100, 400, 700, 1,000, 1,300 and 1,600 mg/L, and the optimum nutritional medium was Zarrouk medium selected from experiment one. Results showed at the initial culture density of 400 mg/L, the highest biomass was gained with the maximum density of 3,071.2 mg/L after 21 d inoculums, with specific growth rate of 9.15% per day and time to double population of 0.076 d. S. platensis biomass from culture in Zarrouk medium at initial cell density 400 mg/L obtained total protein of 51.98%, total lipid of 1.75%, total carbohydrate of 0.75%, carotene of 0.36% and total minerals of 5.2%, on a basis of dry matter.
{"title":"The Effects of Nutritional Media and Initial Cell Density on the Growth and Development of Spirulina platensis","authors":"N. Khánh, N. Diem, L. Nhan, Phan Văn Cư, Trần Quang Khánh Vân, Ngô Thị Hoản","doi":"10.17265/2161-6256/2017.10.008S","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.10.008S","url":null,"abstract":"The main objective of the study was to determine the effects of nutritional media and initial cell density on the growth and development of Spirulina platensis. This study was carried out at the Center for Incubation and Technology Transfer, Hue University from May 2015 to August 2016. In the first experiment, S. platensis was cultured in four different nutritional media, including Zarrouk, Spi-RIA3, Nisole and Thuoc medium (Vietnamese). Resulted showed that S. platensis grew significantly in the Zarrouk medium rather than in other nutritional medium at the same time, and gained the maximum density of 2,736.5 mg/L after 13 d inoculums, with specific growth rate of 4.987% per day and the time to doubling population of 0.139 d. In the second experiment, S. platensis was cultured in six different initial cell densities: 100, 400, 700, 1,000, 1,300 and 1,600 mg/L, and the optimum nutritional medium was Zarrouk medium selected from experiment one. Results showed at the initial culture density of 400 mg/L, the highest biomass was gained with the maximum density of 3,071.2 mg/L after 21 d inoculums, with specific growth rate of 9.15% per day and time to double population of 0.076 d. S. platensis biomass from culture in Zarrouk medium at initial cell density 400 mg/L obtained total protein of 51.98%, total lipid of 1.75%, total carbohydrate of 0.75%, carotene of 0.36% and total minerals of 5.2%, on a basis of dry matter.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87100243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.008
Vladu Marius, Pânzaru Radu Lucian
: In this paper, an analysis of the possibilities of development of milk processing activity was made, using as financing source the public funds provided by the Common Agricultural Policy and the distribution of the investments in Romania. All the EU member states gain 99.6 billion through the European Agricultural Fund for Rural Development, the period 2014-2020, of which Romania has received the total of 8.128 billion euros. Through the 2014-2020 National Rural Development Program (NRDP 2014-2020), sub-measure 4.2 “Support for investment in processing/marketing of agricultural products” (2014), Romania is falling Regulation (EC) 1305/2013 in measure 4 “Investments in physical assets” (2013) and contributes to increasing the value added of products on local markets and inter-professional organizations, the establishment and development of small businesses and job creation. Until now, in the milk processing sector, investments of 93,918,043 euros were made, about 50% (47,667.41 euros) of which from public funds.
{"title":"Investments of Milk Processing Capacities from Romania, through the Funds Provided by the “NPRD 2014-2020”","authors":"Vladu Marius, Pânzaru Radu Lucian","doi":"10.17265/2161-6256/2017.08.008","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.008","url":null,"abstract":": In this paper, an analysis of the possibilities of development of milk processing activity was made, using as financing source the public funds provided by the Common Agricultural Policy and the distribution of the investments in Romania. All the EU member states gain 99.6 billion through the European Agricultural Fund for Rural Development, the period 2014-2020, of which Romania has received the total of 8.128 billion euros. Through the 2014-2020 National Rural Development Program (NRDP 2014-2020), sub-measure 4.2 “Support for investment in processing/marketing of agricultural products” (2014), Romania is falling Regulation (EC) 1305/2013 in measure 4 “Investments in physical assets” (2013) and contributes to increasing the value added of products on local markets and inter-professional organizations, the establishment and development of small businesses and job creation. Until now, in the milk processing sector, investments of 93,918,043 euros were made, about 50% (47,667.41 euros) of which from public funds.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84599796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.005
I. Karaca, M. Avcı, Ö. Güven
Eucalypt-feeding psyllids, Glycaspis brimblecombei Moore (Hemiptera: Psylloidea: Aphalaridae), native to the Australian region, has emerged as an aggressive pest on Eucalyptus in many countries as it was described recently in Greece and Turkey. This study aimed to report this invasive species and its parasitoids on Eucalyptus growing area in Northern Cyprus. G. brimblecombei infested leaves were collected and brought to the laboratory for identification and collection of parasitoids emerged from the pest. In this paper, presence of G. brimblecombei and the parasitoid of the red gum lerp psyllid Psyllaephagus bliteus Riek (Hymenoptera: Encyrtidae) were reported for the first time in Northern Cyprus. Detailed information about the pest and its parasitoids were discussed.
{"title":"Glycaspis brimblecombei Moore (Hemiptera: Aphalaridae), the New Exotic Pest of Eucalyptus in Northern Cyprus","authors":"I. Karaca, M. Avcı, Ö. Güven","doi":"10.17265/2161-6256/2017.08.005","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.005","url":null,"abstract":"Eucalypt-feeding psyllids, Glycaspis brimblecombei Moore (Hemiptera: Psylloidea: Aphalaridae), native to the Australian region, has emerged as an aggressive pest on Eucalyptus in many countries as it was described recently in Greece and Turkey. This study aimed to report this invasive species and its parasitoids on Eucalyptus growing area in Northern Cyprus. G. brimblecombei infested leaves were collected and brought to the laboratory for identification and collection of parasitoids emerged from the pest. In this paper, presence of G. brimblecombei and the parasitoid of the red gum lerp psyllid Psyllaephagus bliteus Riek (Hymenoptera: Encyrtidae) were reported for the first time in Northern Cyprus. Detailed information about the pest and its parasitoids were discussed.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87372881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.003
P. Varma, K. Kumar, V. Sekhar, D. Adilakshmi, M. Suresh, N. Kumar, K. Jayachandra, R. Anitha
Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus (B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis) and Paenibacillus pabuli. There were three antimicrobial peptides (AMPs) producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens (SE1, SE7), B. siamensis (SE4, SE16), B. subtilis (SE2, SE3) and B. velezensis (SE15). The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide (HCN), protease, cellulase and indole acetic acid (IAA). Few strains (SE1 and SE4) were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to Fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.
{"title":"Evaluation of Endophytic Bacteria for Plant Growth Promotion and Pathogen Suppression Traits in Saccharum officinarum","authors":"P. Varma, K. Kumar, V. Sekhar, D. Adilakshmi, M. Suresh, N. Kumar, K. Jayachandra, R. Anitha","doi":"10.17265/2161-6256/2017.08.003","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.003","url":null,"abstract":"Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus (B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis) and Paenibacillus pabuli. There were three antimicrobial peptides (AMPs) producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens (SE1, SE7), B. siamensis (SE4, SE16), B. subtilis (SE2, SE3) and B. velezensis (SE15). The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide (HCN), protease, cellulase and indole acetic acid (IAA). Few strains (SE1 and SE4) were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to Fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"8 23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91211513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.007
C. C. D. Oliveira, A. Moro, L. Ulbricht
This research identified difficulties faced by dairy farmers in family farming, describing this diagnosis through ergonomic demand items (EDIs). The objective was to elaborate an organization model of work related to dairy farming (WRDF). As a methodology, a descriptive exploratory study was carried out in 14 family dairy farms that were visited and had their activities monitored. As main results, 11 EDIs were identified. The ones grouped in the infrastructure factor showed the highest resolution priority. The WRDF model was composed of three categories: management, public policies management and infrastructure, which were further subdivided into technical and social aspects. From this perspective, it is believed that the implementation of the model can bring organizational improvement, once adjustments are made in the procedures and working conditions. This study sheds light on the conditions that need to be resolved with higher priority so that the farmers will remain in the dairy business.
{"title":"Work Organization Model Applied on Smallholder Dairy Farms in Brazil","authors":"C. C. D. Oliveira, A. Moro, L. Ulbricht","doi":"10.17265/2161-6256/2017.08.007","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.007","url":null,"abstract":"This research identified difficulties faced by dairy farmers in family farming, describing this diagnosis through ergonomic demand items (EDIs). The objective was to elaborate an organization model of work related to dairy farming (WRDF). As a methodology, a descriptive exploratory study was carried out in 14 family dairy farms that were visited and had their activities monitored. As main results, 11 EDIs were identified. The ones grouped in the infrastructure factor showed the highest resolution priority. The WRDF model was composed of three categories: management, public policies management and infrastructure, which were further subdivided into technical and social aspects. From this perspective, it is believed that the implementation of the model can bring organizational improvement, once adjustments are made in the procedures and working conditions. This study sheds light on the conditions that need to be resolved with higher priority so that the farmers will remain in the dairy business.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84310887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.004
I. Kepenekçi̇, O. Dura, S. Dura
The aim of this study was to determine the nematicidal effects of some biopesticides againist root-knot nematodes (RKNs) (Meloidogyne incognita) on Bruno type kiwifruit seedlings. Research was conducted in the Atatürk Central Horticultural Research Institute (ACHRI, Yalova, Turkey) greenhouse during 2013-2014. Biopesticides observed in this study were: sesame oil (Devalone EC), castor oil (Ricinus communis), Myrothecium verrucaria (M. verrucaria strain AARC-0255, Inferno DF), Glomus spp. (Endomycorrhizal fungi, Endo Roots Soluble SP) and Paecilomyces lilacinus (Entomopathogenic fungi, P. lilacinus strain PL1, Bionematon SL). Nematicide Nemacur EC 400 (fenamiphos) was used as treated control (TC) and trials were set up as randomized parcel design (RPD) with seven characters and 10 replicates. Reproduction ratio was determined according to scale values in trial applications and second stage juvenile (J2) populations of M. incognita in soil. The results showed that the best nematicidal effects against RKNs were determined in castor oil (77.92% in 2013 and 73.61% in 2014) and P. lilacinus (61.03% in 2013 and 55.55% in 2014).
{"title":"Determination of Nematicidal Effects of Some Biopesticides against Root-Knot Nematode (Meloidogyne incognita) on Kiwifruit","authors":"I. Kepenekçi̇, O. Dura, S. Dura","doi":"10.17265/2161-6256/2017.08.004","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.004","url":null,"abstract":"The aim of this study was to determine the nematicidal effects of some biopesticides againist root-knot nematodes (RKNs) (Meloidogyne incognita) on Bruno type kiwifruit seedlings. Research was conducted in the Atatürk Central Horticultural Research Institute (ACHRI, Yalova, Turkey) greenhouse during 2013-2014. Biopesticides observed in this study were: sesame oil (Devalone EC), castor oil (Ricinus communis), Myrothecium verrucaria (M. verrucaria strain AARC-0255, Inferno DF), Glomus spp. (Endomycorrhizal fungi, Endo Roots Soluble SP) and Paecilomyces lilacinus (Entomopathogenic fungi, P. lilacinus strain PL1, Bionematon SL). Nematicide Nemacur EC 400 (fenamiphos) was used as treated control (TC) and trials were set up as randomized parcel design (RPD) with seven characters and 10 replicates. Reproduction ratio was determined according to scale values in trial applications and second stage juvenile (J2) populations of M. incognita in soil. The results showed that the best nematicidal effects against RKNs were determined in castor oil (77.92% in 2013 and 73.61% in 2014) and P. lilacinus (61.03% in 2013 and 55.55% in 2014).","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82530341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.001
M. Mofokeng, H. Araya, P. Mashela, C. P. D. Plooy
: Water is an important factor affecting growth, yield and distribution of different species. Plant response to water deficit can be in the form of physiological disorders, such as reduction in transpiration or assimilating partitioning to root growth. Sustainable use of water has become a priority in agriculture and thus innovative irrigation management practices are critical. The study aimed at investigating how watering frequency and terminal water stress influence growth of Pelargonium sidoides , an important medicinal plant in Southern Africa. The trial was a randomized complete block design with three replicates, and treatment factors were watering frequency (everyday, twice and once a week) and terminal water stress (no watering four weeks before harvesting, no watering two weeks before harvesting and no terminal stress). There was an interacting effect of watering frequency and terminal water stress on biomass and fresh root yield. More frequent watering resulted in significantly higher biomass and fresh root yield, compared to other treatments. Watering everyday with terminal or no terminal water stress resulted in higher fresh root yield, compared to other watering treatments with terminal water stress. Plant height and leaf area were significantly affected by watering frequency and terminal water stress, respectively. A significant drop in stomatal conductance of plants watered everyday was observed 240 d after treatment implementation, such that there was no significant difference across all the three watering frequency treatments. In conclusion, farmers can save on irrigation costs by reducing watering frequency, as there was no significant difference on dry root yield.
{"title":"Stomatal Conductance, Growth and Yield of Pelargonium sidoides DC. in Response to Watering Frequency and Terminal Water Stress","authors":"M. Mofokeng, H. Araya, P. Mashela, C. P. D. Plooy","doi":"10.17265/2161-6256/2017.08.001","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.001","url":null,"abstract":": Water is an important factor affecting growth, yield and distribution of different species. Plant response to water deficit can be in the form of physiological disorders, such as reduction in transpiration or assimilating partitioning to root growth. Sustainable use of water has become a priority in agriculture and thus innovative irrigation management practices are critical. The study aimed at investigating how watering frequency and terminal water stress influence growth of Pelargonium sidoides , an important medicinal plant in Southern Africa. The trial was a randomized complete block design with three replicates, and treatment factors were watering frequency (everyday, twice and once a week) and terminal water stress (no watering four weeks before harvesting, no watering two weeks before harvesting and no terminal stress). There was an interacting effect of watering frequency and terminal water stress on biomass and fresh root yield. More frequent watering resulted in significantly higher biomass and fresh root yield, compared to other treatments. Watering everyday with terminal or no terminal water stress resulted in higher fresh root yield, compared to other watering treatments with terminal water stress. Plant height and leaf area were significantly affected by watering frequency and terminal water stress, respectively. A significant drop in stomatal conductance of plants watered everyday was observed 240 d after treatment implementation, such that there was no significant difference across all the three watering frequency treatments. In conclusion, farmers can save on irrigation costs by reducing watering frequency, as there was no significant difference on dry root yield.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74166406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.006
A. Kelomey, A. Paraïso, H. Sina, H. Legout, A. Adjanohoun, L. Garnery, L. Baba-Moussa
: The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA (mtDNA) as a molecular marker in their cytochrome c oxidase subunit I and II ( COI-COII ) intergenic region. A total of 304 bee colonies were sampled in 27 municipalities of the cashew growing area of Benin. These samples were analyzed by the cleaved amplified polymorphisms technique for determining the haplotypes of subspecies present in the sampled population . Eight PCR-RFLP profiles of African lineage A were then identified in the 304 samples of bees investigated. Forty-nine percent (49%) of the samples showed the profile of haplotype A1 (subspecies adansonii of Zambia), 40% of haplotype A4 (subspecies scutellata of South Africa) and 3% of haplotype A19 (subspecies adansonii of Guinea). Five other haplotypes of the African branch (A) that had been described in a previous study were also identified: new 1 (2%), new 2 (2%), new 3 (1%), new 4 (2%) and new 5 (1%). This study showed that A. mellifera from Benin belonged only to lineage A with the predominance of haplotypes A1 and A4. This study will contribute to the development of coherent policies for conservation of local bees in Benin.
{"title":"Genetic Variability of the Mitochondrial DNA in Honeybees (Apis mellifera L.) from Benin","authors":"A. Kelomey, A. Paraïso, H. Sina, H. Legout, A. Adjanohoun, L. Garnery, L. Baba-Moussa","doi":"10.17265/2161-6256/2017.08.006","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.006","url":null,"abstract":": The aim of this study was to evaluate the genetic variability in bees Apis mellifera from Benin by using mitochondrial DNA (mtDNA) as a molecular marker in their cytochrome c oxidase subunit I and II ( COI-COII ) intergenic region. A total of 304 bee colonies were sampled in 27 municipalities of the cashew growing area of Benin. These samples were analyzed by the cleaved amplified polymorphisms technique for determining the haplotypes of subspecies present in the sampled population . Eight PCR-RFLP profiles of African lineage A were then identified in the 304 samples of bees investigated. Forty-nine percent (49%) of the samples showed the profile of haplotype A1 (subspecies adansonii of Zambia), 40% of haplotype A4 (subspecies scutellata of South Africa) and 3% of haplotype A19 (subspecies adansonii of Guinea). Five other haplotypes of the African branch (A) that had been described in a previous study were also identified: new 1 (2%), new 2 (2%), new 3 (1%), new 4 (2%) and new 5 (1%). This study showed that A. mellifera from Benin belonged only to lineage A with the predominance of haplotypes A1 and A4. This study will contribute to the development of coherent policies for conservation of local bees in Benin.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81406942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-28DOI: 10.17265/2161-6256/2017.08.002
M. R. Mora, P. J. Gómez, C. Valbuena, A. Probanza
: In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques (use of fertilizers, irrigation with river water) may have on the rhizospheric microbiome of Vitis vinifera plants. Therefore, it is postulated the Cenophenoresistome as a novel methodology to evaluate complex communities’ global resistance against different antibiotics, by using and adjusting a serial of techniques traditionally applied to evaluate a monospecific population’s resistance against antibiotics (Vitek ® , ATB ® and disk diffusion methods). Likewise, the metabolic profile (CLPP: comunity level physiological profile) of bacterial communities is studied by Biolog ECO ® . In relation to the functional structure of the bacterial communities, it is observed that the metabolic profile (diversity, kinetics and CLPP) of unexploited soils differs from soils under anthropic influence. It is discussed the causes of resistance in the human clinic antibiotic treatment based on the agrarian management, especially with the contamination transmitted by irrigation water, which could be associated with changes in edaphic communities. The results obtained in the present study through two different approaches ( Cenophenoresistome and metabolic profiles) are consistent with each other, suggesting that both methods can be good bioindicators of the state of humankind-altered soils that host natural ecosystems. Likewise, the concept of Cenophenoresistome is proposed as a bioindicator of soil response to alteration processes, as well as a possible predictor of its evolution in edaphic remediation processes. Bacterial communities, antibiotic resistance, Cenophenoresistome , Vitis vinifera , comunity level physiological profile, Biolog ECO.
{"title":"Effect of the Type of Vitis vinifera Cultivation in the Cenophenoresistome and Metabolic Profiling (CLPP) of Edaphic Bacterial Communities","authors":"M. R. Mora, P. J. Gómez, C. Valbuena, A. Probanza","doi":"10.17265/2161-6256/2017.08.002","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.08.002","url":null,"abstract":": In the present work, bacterial soil communities of different grapevine exploitation samples are studied in order to elucidate the possible influence of different agrarian management techniques (use of fertilizers, irrigation with river water) may have on the rhizospheric microbiome of Vitis vinifera plants. Therefore, it is postulated the Cenophenoresistome as a novel methodology to evaluate complex communities’ global resistance against different antibiotics, by using and adjusting a serial of techniques traditionally applied to evaluate a monospecific population’s resistance against antibiotics (Vitek ® , ATB ® and disk diffusion methods). Likewise, the metabolic profile (CLPP: comunity level physiological profile) of bacterial communities is studied by Biolog ECO ® . In relation to the functional structure of the bacterial communities, it is observed that the metabolic profile (diversity, kinetics and CLPP) of unexploited soils differs from soils under anthropic influence. It is discussed the causes of resistance in the human clinic antibiotic treatment based on the agrarian management, especially with the contamination transmitted by irrigation water, which could be associated with changes in edaphic communities. The results obtained in the present study through two different approaches ( Cenophenoresistome and metabolic profiles) are consistent with each other, suggesting that both methods can be good bioindicators of the state of humankind-altered soils that host natural ecosystems. Likewise, the concept of Cenophenoresistome is proposed as a bioindicator of soil response to alteration processes, as well as a possible predictor of its evolution in edaphic remediation processes. Bacterial communities, antibiotic resistance, Cenophenoresistome , Vitis vinifera , comunity level physiological profile, Biolog ECO.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89159165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-28DOI: 10.17265/2161-6256/2017.07.005
Johanis Ly, O. Sjofjan, I. H. Djunaidi, S. Suyadi
Twenty four first weaned sows Duroc × Landrace × Veredeld Duits Landvarken (VDL), average live weight 135-190 kg (11% coefficient of variation (CV)) were allocated to four treatments (six animals/group/treatment) in a randomized block design to evaluate the effect of fermented tamarind seeds (FTS) supplementation during estrus to lactation period on the performance of sows and weaned piglets. There were four treatment diets offered: basal diet (T0), T0 + 5% FTS (T1), T0 + 7.5% FTS (T2) and T0 + 10% FTS (T3). Supplementing sows with 5%-10% FTS increased daily feed intake at all stages and 10% FTS supplementation level performed the highest (P < 0.05) at all stages of pregnancy. Litter size increased 1-2 piglets/sow, piglets born alive increased 1.3-3 piglets/sow, piglet birth weight increased 10-160 g/piglet and weaned piglets increased 3.3-5.3 piglets/sow, while mortality was reduced of 18%-27% per sow. Inclusion of FTS at level of 10% in the diet of sows had the highest daily live weight gain of piglets during four suckling weeks (P < 0.05). However, there was no significant difference (P < 0.05) among treatments on daily live weight gain of piglets at week 1-2. This study revealed that supplementing sows with FTS during estrus to lactation period improved sows’ and piglets’ performances, and reduced piglets’ mortality up to 29% at weaning.
{"title":"Effect of Fermented Tamarind Seeds (FTS) Supplementation to Sows during Estrus to Lactation Period on Sow Reproduction Performances and Piglet Mortality at Weaning","authors":"Johanis Ly, O. Sjofjan, I. H. Djunaidi, S. Suyadi","doi":"10.17265/2161-6256/2017.07.005","DOIUrl":"https://doi.org/10.17265/2161-6256/2017.07.005","url":null,"abstract":"Twenty four first weaned sows Duroc × Landrace × Veredeld Duits Landvarken (VDL), average live weight 135-190 kg (11% coefficient of variation (CV)) were allocated to four treatments (six animals/group/treatment) in a randomized block design to evaluate the effect of fermented tamarind seeds (FTS) supplementation during estrus to lactation period on the performance of sows and weaned piglets. There were four treatment diets offered: basal diet (T0), T0 + 5% FTS (T1), T0 + 7.5% FTS (T2) and T0 + 10% FTS (T3). Supplementing sows with 5%-10% FTS increased daily feed intake at all stages and 10% FTS supplementation level performed the highest (P < 0.05) at all stages of pregnancy. Litter size increased 1-2 piglets/sow, piglets born alive increased 1.3-3 piglets/sow, piglet birth weight increased 10-160 g/piglet and weaned piglets increased 3.3-5.3 piglets/sow, while mortality was reduced of 18%-27% per sow. Inclusion of FTS at level of 10% in the diet of sows had the highest daily live weight gain of piglets during four suckling weeks (P < 0.05). However, there was no significant difference (P < 0.05) among treatments on daily live weight gain of piglets at week 1-2. This study revealed that supplementing sows with FTS during estrus to lactation period improved sows’ and piglets’ performances, and reduced piglets’ mortality up to 29% at weaning.","PeriodicalId":14977,"journal":{"name":"Journal of agricultural science & technology A","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78189523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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