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Non-starch Polysaccharide Degrading Enzymes in Corn and Wheat-Based Broiler Diets: Dual Activity for Major Substrates 玉米和小麦肉鸡饲粮中非淀粉多糖降解酶:对主要基质的双重活性
Pub Date : 2018-02-28 DOI: 10.17265/2161-6256/2018.02.004
K. D. Keyser, N. Dierick, Leni Kuterna, Olivier Maigret, S. Kaczmarek, A. Rutkowski, E. Vanderbeke
This study consolidates the efficacy of a non-starch polysaccharide (NSP)ase enzyme-concept in corn and wheat-based broiler diets. The NSP building blocks of corn, wheat and soybean meal (SBM, 44% protein) were studied first. Cereal NSP primarily consists of arabinose, xylose and glucose molecules that form arabinoxylan, β-glucan and cellulose polysaccharides. In soybean meal (SBM), glucose (cellulose) predominates, next to galactose and uronic acids that form “pectins”. Broiler performance progress using in-feed NSPase enzymes depends on the concentration, complexity and solubility of the substrate and the genetic potential of the animal, mainly. A dual NSPase enzyme-concept for cereal and SBM NSP, predominantly being arabinoxylan, β-glucan and cellulose, was developed. Methods for measuring enzyme activities (endo-1,4-β-xylanase (EC 3.2.1.8) and endo-1,3(4)-β-glucanase (EC 3.2.1.6)) were developed (AVEVE Biochem UNITS) and preparations thereof (XG) standardized to meet requirements for most challenging NSP (corn, barely soluble). Feed intake (FI), bodyweight gain (BWG) and feed:gain ratio (F:G) were assessed in three zootechnical studies, each using 160 Ross 308 broilers split in two feeding groups with 80 birds/group (10 replicates of eight) for 42 d (starter/grower period). Respective corn-SBM, wheat-SBM and corn/wheat-SBM diets were used as negative control (NC) or added with the enzyme-concept (XG). In the total period, XG ameliorated BWG and F:G compared to NC in each study, where BWG increased best in diets with corn and F:G lowered most in diets with wheat. The dual NSPase enzyme-concept offers ingredient flexibility in present setting by enhancing the nutritional content of corn, wheat and SBM, expectedly from cleaving major NSP target molecules. Thereby, broiler professionals increase feed formulation liberty and safety and production result simultaneously.
本研究验证了非淀粉多糖(NSP)酶概念在玉米和小麦型肉鸡饲粮中的有效性。首先研究了玉米、小麦和豆粕(SBM, 44%蛋白质)的NSP构建块。谷物NSP主要由阿拉伯糖、木糖和葡萄糖分子组成,形成阿拉伯木聚糖、β-葡聚糖和纤维素多糖。在豆粕(SBM)中,葡萄糖(纤维素)占主导地位,其次是半乳糖和形成“果胶”的醛酸。肉鸡在饲料中使用NSPase酶的生产性能进展主要取决于底物的浓度、复杂性和溶解度以及动物的遗传潜力。提出了谷类和谷类的双NSPase酶概念,主要是阿拉伯木聚糖、β-葡聚糖和纤维素。建立了酶活性测定方法(endo1,4 -β-木聚糖酶(EC 3.2.1.8)和endo1,3 (4)-β-葡聚糖酶(EC 3.2.1.6)) (AVEVE Biochem UNITS),并对其制剂(XG)进行了标准化,以满足最具挑战性的NSP(玉米,几乎不溶)的要求。采用3项动物技术试验,对采食量(FI)、体重增重(BWG)和料重比(F:G)进行评估,每项试验选用160只罗斯308肉鸡,分为2个饲喂组,每组80只(10个重复,每组8只),饲喂42 d(发育期/生长期)。分别以玉米- sbm、小麦- sbm和玉米/小麦- sbm为阴性对照(NC)或添加酶概念(XG)。在整个周期内,与NC相比,XG改善了体增重和F:G,其中玉米饲粮中体增重增加最多,小麦饲粮中F:G降低最多。双NSPase酶的概念通过提高玉米、小麦和SBM的营养成分,在目前的环境下提供了成分的灵活性,预计将通过切割主要的NSP靶分子。从而使肉鸡专业人员同时提高饲料配方的自由度和安全性及生产效果。
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引用次数: 3
Evaluation of Mastitis Related Measures & Their Applications to Classify Buffalo Milk in Chitwan, Nepal 尼泊尔奇旺地区乳腺炎相关措施评价及水牛奶分类应用
Pub Date : 2018-02-28 DOI: 10.17265/2161-6256/2018.02.006
I. P. Dhakal, H. Nagahata
A study was performed to evaluate the epidemiological aspects of buffalo mastitis in the District Chitwan, Nepal for characterizing the California mastitis test (CMT), somatic cell count (SCC), electrical conductivity (EC) values and bacteriological analysis for defining buffalo milk. The CMT was performed by mixing equal volume of milk and 3% sodium lauryl sulphate. The SCC was determined by staining milk film with New Man’s Lampert Stain and EC values were measured by manual digital mastitis detector and expressed as mS/cm. Bacteriological analysis was done on the basis of Gram’s stain, morphological findings, colony characteristics and biochemical tests. The maximum number (16%) of clinical cases of mastitis was observed in the month of July and lowest in the month of April (1.6%). When the temperature and humidity increased, it indicates that there is need for better care of lactating buffaloes during this month. On a quarter basis, 16% of the foremilk samples in buffaloes were diagnosed as having subclinical mastitis and 11% were diagnosed as having clinical mastitis. The results of CMT scores and SCC showed the evidence that subclinical and clinical mastitic milk was having CMT positive scores (+1~+3) with ≥ 200 × 10 cells/mL. The mean pH of clinically normal buffalo milk was 6.75 (range 6.39 to 7.08) and subclinical mastitic and clinical mastitic milk was 6.85 (range 6.37 to 7.10) and 6.88 (range 6.41 to 7.20), respectively. Analysis of EC value in the milk revealed the presence of mastitis in buffaloes and the cut-off values was 3.7 mS/cm. The coagulase negative Staphylococcus (CNS), such as S. albus and S. epidermidis were the predominant organisms associated with subclinical mastitis, and CNS and coliforms in clinical mastitis. This information suggests that environmental mastitis was prevalent in buffaloes of Chitwan District. In this study, 9.5% of the quarters were having bacterial count (BC) more than 250 cfu/mL. The proposed criteria for normal milk are absence of clinical signs, CMT negative, SCC < 200 × 10 cells/mL, EC < 3.7 mS/cm and < 250 cfu/mL bacteria. The parameters for defining subclinically mastitic milk are absence of clinical signs, CMT positive, SCC ≥ 200 × 10 cells/mL, EC > 3.7 mS/cm and > 250 cfu/mL bacteria. Similarly, clinical mastitic milk was defined as milk having presence of clinical signs, CMT positive, SCC ≥ 200 × 10 cells /mL, EC > 3.7 mS/cm and BC > 250 cfu/mL.
为了评估尼泊尔Chitwan地区水牛乳腺炎的流行病学方面的特征,进行了一项研究,以确定加州乳腺炎试验(CMT)、体细胞计数(SCC)、电导率(EC)值和细菌学分析,以确定水牛奶。将等量牛奶和3%十二烷基硫酸钠混合进行CMT。用新曼氏兰伯特染色法对乳膜进行SCC测定,用手动数字乳腺炎检测器测定EC值,并用mS/cm表示。根据革兰氏染色、形态学、菌落特征和生化试验进行细菌学分析。乳腺炎临床病例以7月最多(16%),4月最少(1.6%)。当温度和湿度增加时,表明在这个月需要更好地照顾泌乳水牛。在四分之一的基础上,16%的水牛前乳样本被诊断为亚临床乳腺炎,11%被诊断为临床乳腺炎。CMT评分和SCC结果显示,亚临床和临床乳乳均有CMT阳性评分(+1~+3),≥200 × 10个细胞/mL。临床正常水牛奶的平均pH值为6.75(范围6.39至7.08),亚临床乳腺炎和临床乳腺炎牛奶的平均pH值分别为6.85(范围6.37至7.10)和6.88(范围6.41至7.20)。牛奶中的EC值分析显示水牛存在乳腺炎,临界值为3.7 mS/cm。亚临床乳腺炎以凝固酶阴性葡萄球菌(CNS)如白色葡萄球菌和表皮葡萄球菌为主,临床乳腺炎以CNS和大肠菌群为主。这一信息表明环境性乳腺炎在奇旺地区的水牛中普遍存在。在本研究中,9.5%的季度细菌计数(BC)超过250 cfu/mL。建议的正常乳汁标准为无临床症状、CMT阴性、SCC < 200 × 10个细胞/mL、EC < 3.7 mS/cm和细菌< 250 cfu/mL。定义亚临床乳腺炎的参数为无临床体征、CMT阳性、SCC≥200 × 10个细胞/mL、EC > 3.7 mS/cm、细菌> 250 cfu/mL。同样,临床乳汁定义为存在临床体征,CMT阳性,SCC≥200 × 10细胞/mL, EC > 3.7 mS/cm, BC > 250 cfu/mL。
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引用次数: 7
Assessment of Climate Change Variability Impacts on Wheat and Barley Production in Palestine 气候变化变率对巴勒斯坦小麦和大麦生产影响的评估
Pub Date : 2018-02-28 DOI: 10.17265/2161-6256/2018.02.002
Imadeddin Albaba
The study aimed at addressing climate variability impacts on wheat and barley production in Palestine. A combination of literature survey and multiple semi-structured interviews with officials from the Palestinian governmental agriculture related agencies and active non-governmental organizations (NGOs) used for gathering information on wheat and barley production in Palestine. The gathered data along with information and subsequent results prevailed that the West Bank since the 1970s has experienced a significant decrease in winter rainfall. Across 15 sites, growing season rainfall (September to May) decreased by an average of 11% and the sum of rainfall in September and October decreased by 45%. Despite the large decline in rainfall, yields based on the actual weather data did not fall. These results were due to the rainfall changes mainly occurring in September and October, a period when rainfall often is less than crop demand. The study arrived at conclusion based on the study results that climate change variabilities have differential impacts on the yield growth of wheat and barley. However, both rainfed dependent crops are adversely affected by the current climate trends. The yields of barley and wheat are decreasing due to increased temperature and decreased precipitation.
该研究旨在解决气候变化对巴勒斯坦小麦和大麦生产的影响。文献调查和与巴勒斯坦政府农业相关机构和活跃的非政府组织官员的多次半结构化访谈相结合,用于收集巴勒斯坦小麦和大麦生产的信息。所收集的数据以及资料和后来的结果表明,自1970年代以来,西岸的冬季降雨量显著减少。在15个站点中,生长季节降雨量(9月至5月)平均减少了11%,9月和10月的降雨量总和减少了45%。尽管降雨量大幅下降,但基于实际天气数据的产量并未下降。这些结果是由于降雨变化主要发生在9月和10月,这一时期的降雨量往往低于作物需求。根据研究结果,得出了气候变化变异对小麦和大麦产量增长有不同影响的结论。然而,这两种依赖雨养的作物都受到当前气候趋势的不利影响。由于气温升高和降水减少,大麦和小麦的产量正在下降。
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引用次数: 2
The Localization of UK Experience in Environmental Education in Fairy Lake Botanical Garden, Shenzhen, China 英国环境教育经验在深圳仙湖植物园的本土化
Pub Date : 2018-02-28 DOI: 10.17265/2161-6256/2018.02.001
Shan Li, Qing Wang, Tao Wang
In China, the environmental education (EE) is a brief new but popular topic in recent several years as a method to cope with the gap between nature and children. EE was started in UK with a long-term development and the useful experience can be utilized for the Chinese EE development. As a case study, the experiences of UK organizations were summarized and applied in the EE system in Fairy Lake Botanical Garden in Shenzhen (SZBG), China. The localization of UK’s experience in the EE development in SZBG in terms of EE program, course content and resource was summarized in this paper. There are also discussions for the future update of EE in SZBG and for other local organizations like botanical gardens, wetland parks, nature reserve etc.
在中国,环境教育(EE)作为一种应对自然与儿童之间差距的方法,是近年来一个简短而热门的话题。电子商务始于英国,有着长期的发展历程,其经验对中国电子商务的发展具有借鉴意义。以中国深圳仙湖植物园为例,总结了英国机构的经验,并将其应用到EE系统中。本文从英语教学项目、课程内容、资源等方面总结了英国英语教学的本土化经验。会议还讨论了未来SZBG和其他当地组织(如植物园、湿地公园、自然保护区等)的EE更新。
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引用次数: 0
Detection of Acetyl-CoA Carboxylase (ACCase) Inhibitor Herbicides Resistance in Sterile Wild Oat (Avena sterilis L.) Using Agar Quick Test 野生不育燕麦乙酰辅酶a羧化酶(ACCase)抑制剂除草剂抗性检测使用琼脂快速测试
Pub Date : 2018-01-28 DOI: 10.17265/2161-6256/2018.01.002
Abdullatief Mohammed Abdurruhman, S. Uygur, F. Uygur
This study was conducted using seeds in Petri dish containing agar medium in order to determine acetyl-CoA carboxylase (ACCase) herbicides resistance (R) in Avena sterilis that was grown in wheat fields at Adana province, Turkey. Seeds were collected from one large suspected field, where clodinafop-propargyl (Aryloxyphenoxypropionate “FOPs”) and pinoxaden (Phenylpyrazoline “DEN”) have been applied for many years. Susceptible (S) population was collected from the road side on the same region. Agar media of concentration 14 g/L was prepared and it was melted in microwave. Then the amount of 20 mL agar media was added into each Petri dish. Five seeds were placed on agar mediums containing discriminating dose of clodinafop and pinoxaden. Petri dishes were placed in growth incubator operating at 10 °C. After 15 d, both radicle and hypocotyl length were measured. The percentage of germinated seed and dose-response curves were determined. At these different concentration levels, there were more than 50% of R and less than 40% of S seed germinated for pinoxaden. However, for clodinafop, more than 60% of R and less than 50% of S seeds were germinated. At higher concentration levels, the populations of resistant and susceptible were not germinated for both herbicides. The resistance value of R population was then compared with that of the S biotype. From the resistance index (RI), the population was more resistant to pinoxaden (7.43 for radicle and 2.47 for hypocotyl) than the clodinafop-propagyl (1.39 for radicle and 3.77 for hypocotyl). The method provided a simple, quick and cost effective way to identify ACCase herbicides resistance in most grass weeds.
为了测定在土耳其阿达纳省的麦田中种植的麦草对乙酰辅酶a羧化酶(ACCase)除草剂的抗性(R),本研究在含有琼脂培养基的培养皿中对麦草的种子进行了研究。种子采集自一大片疑似农田,该农田多年来一直使用氯硝福丙炔(芳基苯氧丙酸酯“FOPs”)和苯吡唑啉“DEN”。在同一地区的路边采集易感人群。制备浓度为14 g/L的琼脂培养基,微波融化。然后在每个培养皿中加入20 mL琼脂培养基。将5粒种子置于含有克地那福和匹诺莎鉴别剂量的琼脂培养基上。培养皿置于生长培养箱中,温度为10℃。15d后,测量胚根和下胚轴长度。测定了种子发芽率和剂量-反应曲线。在不同浓度水平下,匹诺沙登的R种子萌发率大于50%,S种子萌发率小于40%。而对于clodinafop, R种子的萌发率超过60%,S种子的萌发率不到50%。在较高浓度下,两种除草剂的抗性和敏感群体均不萌发。然后将R群体的抗性值与S生物型的抗性值进行比较。从抗性指数(RI)看,种群对匹诺沙登的抗性(根茎7.43,下胚轴2.47)高于对氯地那福-繁殖虫的抗性(根茎1.39,下胚轴3.77)。该方法为大多数禾本科杂草的ACCase抗性鉴定提供了一种简单、快速、经济的方法。
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引用次数: 2
Additive Effects of Water-Soluble Propolis (Greit 120) and Human Interferon Alfa (HuIFN-αN3) against Influenza Viruses in Vitro 水溶性蜂胶(Greit 120)与人α干扰素(HuIFN-αN3)体外抗流感病毒的加性作用
Pub Date : 2018-01-28 DOI: 10.17265/2161-6256/2018.01.005
Filipič Bratko, Gradišnik Lidija, Pereyra Adriana, Kopinč Rok, Rihar Klemen, Ružić-Sabljić Eva, K. Snežana, Đermić Damir, Šooš Eugen, Volpi Nicola, F. Alfredo, H. Mazija
Influenza virus affects the respiratory tract in humans causing a range of distinct manifestations including fever, nasal secretions, cough, headaches, muscle pain and pneumonia, which could become violent and severe. It was found that influenza A viruses remain resistant to amantadine and rimantadin with high level of oseltamvir resistance. Therefore, there is a need for constant improvement of drugs active against resistant influenza viruses. Propolis has anti-influenza activity both in vitro and in vivo. Human leukocyte interferon (HuIFN-αN3) is a multi- subtype protein that displays an antiviral activity against influenza virus. In this study we elucidated the anti-influenza activity of the mixes of water-soluble propolis (WSP) (Greit 120) and HuIFN-αN3 at different ratios. Greit 120 polyphenols, total phenol acids and bioflavonoid were characterized by HPLC-UV-ESI- MS504971 and HuIFN-αN3 by reverse-phase high- performance liquid chromatography (RP-HPLC). Influenza A and B viruses were separately added to the LLC-MK2 cells treated with WSP (Greit 120) and HuIFN-αN3 alone or in proportions 1:1, 1:2 and 2:1. Plates were incubated and cytopathic effect was determined. The best results (ID50) were obtained with the mix of 10% WSP and HuIFN-αN3 in proportion 1:2, showing ID50 at 12 ± 0.2 μg/mL and 19 ± 0.6 μg/mL for influenza A and B viruses, respectively. When comparing anti- influenza activity of WSP (Greit 120)/HuIFN-αN3 with that of ribavirin, it was found that 1:2 was the optimal ratio for WSP (Greit 120)/HuIFN-αN3 (0.5 and 0.6 for influenza A and B, respectively). This new formulation of WSP (Greit 120) and HuIFN-αN3, showing better anti-Influenza activity, will definitely improve its application in flu infections.
流感病毒影响人类呼吸道,引起一系列明显的症状,包括发烧、鼻分泌物、咳嗽、头痛、肌肉疼痛和肺炎,这些症状可能变得剧烈和严重。发现甲型流感病毒对金刚烷胺和金刚乙胺仍然具有耐药性,对奥司他韦具有高度耐药性。因此,有必要不断改进对耐药流感病毒有效的药物。蜂胶在体内和体外均具有抗流感活性。人白细胞干扰素(HuIFN-α n3)是一种对流感病毒具有抗病毒活性的多亚型蛋白。本实验研究了水溶性蜂胶(WSP) (Greit 120)与惠氏蛋白-αN3以不同比例混合后的抗流感活性。采用HPLC-UV-ESI- MS504971和反相高效液相色谱(RP-HPLC)对Greit 120多酚、总酚酸和生物类黄酮进行了表征。分别用WSP (Greit 120)和HuIFN-αN3单独或按1:1、1:2和2:1的比例添加甲型流感病毒和乙型流感病毒到lc - mk2细胞中。培养皿孵育,测定细胞病变效应。10% WSP与HuIFN-αN3按1:2的比例混合,对甲型流感病毒和乙型流感病毒的ID50分别为12±0.2 μg/mL和19±0.6 μg/mL。比较WSP (Greit 120)/HuIFN-α n3与利巴韦林的抗流感活性,发现WSP (Greit 120)/HuIFN-α n3的最佳配比为1:2(甲型流感0.5,乙型流感0.6)。该新配方的WSP (Greit 120)和HuIFN-αN3具有较好的抗流感活性,必将提高其在流感感染中的应用。
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引用次数: 0
Assessment of Fruit Fly Trapping System in Mango Orchards in Northern Côte d’Ivoire Côte科特迪瓦北部芒果果园捕蝇系统评价
Pub Date : 2018-01-28 DOI: 10.17265/2161-6256/2018.01.003
M. Y. Minhibo, O. R. N'Dépo, N. Hala, H. Koua, Yalamoussa Tuo, A. N’goran, A. Coulibaly, S. Doumbia, J. A. Djaha, C. Kouakou, A. N. Adopo, H. A. Nda, H. Djidji
Mango is the third largest export fruit in Côte d’Ivoire. However, its yield is limited by the damage caused by fruit flies (Diptera: Tephritidae). This study aimed at assessing the trapping system for fruit fly detection and monitoring in mango orchards in Northern Côte d’Ivoire. Four sites were selected in Northern Côte d’Ivoire in mango production area and in timber yard areas. Five trapping systems were tested: two food attractants (torula yeast, CeraTrap) and three sex attractants (methyl eugenol, trimedlure, cue-lure). Plastic bottles were used for food attractants and commercial traps for sex attractants. Five traps per treatment with three repetitions per site were set up. Ten species of flies have been identified: Bactrocera dorsalis, B. cucurbitae, Ceratitis anonae, C. bremii, C. capitata, C. cosyra, C. punctata, Dacus ciliatus, D. punctatifrons and Dacus spp.. Among these species, B. dorsalis was dominant. It was captured by 94.04% and 90.94%, respectively, in mango orchards and forest areas included wild fruits. The average number of individuals captured varied from one species to another, depending on the attractants. This average was estimated to 379.91, 27.58, 25.55, 15.1 and 11.93, respectively, for méthyl eugénol, torula yeast, CeraTrap, cue-lure, trimedlure. The attractants have shown their specificity in capturing fly species. Methyl eugenol had the highest capture and was the most effective.
芒果是Côte科特迪瓦第三大出口水果。然而,其产量受到果蝇(双翅目:蝗科)危害的限制。本研究旨在评估Côte科特迪瓦北部芒果果园中果蝇检测和监测的诱捕系统。在Côte科特迪瓦北部芒果生产区和木材场区选定了四个场址。试验了5种诱捕系统:2种食物引诱剂(torula yeast, CeraTrap)和3种性引诱剂(methyl eugenol, trimedlure, cue-lure)。食品引诱剂用塑料瓶,性引诱剂用商业诱捕器。每次处理设置5个诱捕器,每个部位重复3次。已鉴定蝇类10种:桔小实蝇、葫芦小实蝇、蝇蛆、短尾小实蝇、头型小实蝇、尾型小实蝇、点状小实蝇、纤毛小实蝇、点状小实蝇和大实蝇。在这些种中,桔粉蚧占优势地位。在芒果果园和有野果的林区,捕获率分别为94.04%和90.94%。根据引诱剂的不同,捕获的平均个体数因物种而异。估计msamthyl - egsamol、torula酵母、CeraTrap、cue-lure、trimedlure的平均值分别为379.91、27.58、25.55、15.1和11.93。诱蚊剂对蝇类具有特异性。甲基丁香酚的捕集量最高,效果最好。
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引用次数: 8
Effect of Parapheromones on the Capture of Fruit Flies (Diptera: Tephritidae) in Burundi 副肾上腺素对布隆迪地区果蝇(双翅目:蝗科)捕获的影响
Pub Date : 2017-12-28 DOI: 10.17265/2161-6256/2017.06.007
L. Ndayizeye, B. Nzigidahera, C. D. Theron
This study aimed to make an inventory of fruit fly species in Burundi, by use of parapheromones. The trapping by parapheromones is a method used to capture fruit fly species. Four types of parapheromones were used, namely, methyl eugenol, cuelure, terpinyl acetate and trimedlure. In total, 70,115 individuals were captured, of which, 68,728 individuals (or 98.02%) were fruit flies, while 1,387 individuals (1.98%) were non-fruit flies. The majority of Tephritidae has been captured by methyl eugenol with 62,538 individuals (90.97%), followed by cuelure with 3,621 individuals (5.27%). Terpinyl acetate and trimedlure have captured 1,900 and 669 individuals, respectively. The effectiveness of parapheromones has varied from one locality to another. In fact, methyl eugenol showed efficiency in the city of Bujumbura, Rumonge, Kigwena and Cibitoke located at an altitude between 780 m and 887 m; cuelure in Bubanza and Kayanza at an altitude between 1,101 m and 2,219 m; trimedlure and terpinyl acetate at Mwaro at an altitude of 1,640 m. From the point of view of abundance of species caught, Bactrocera dorsalis dominates with 62,349 individuals (90.72%), and it is in the Bujumbura city that this species has been captured in abundance.
本研究的目的是利用旁信息素对布隆迪的果蝇物种进行清查。旁信息素诱捕法是一种捕捉果蝇的方法。使用了甲基丁香酚、培养物、醋酸松品酯和三叶草四种副激素。共捕获果蝇70115只,其中果蝇68728只(98.02%),非果蝇1387只(1.98%)。甲基丁香酚捕蝇最多,捕蝇62538只(90.97%),其次为培养捕蝇3621只(5.27%)。乙酸松品酯和三叶虫分别捕获了1,900和669只。副信息素的功效因地而异。事实上,甲基丁香酚在海拔780 ~ 887 m的布琼布拉、鲁蒙格、基格韦纳和奇比托克等城市表现出效率;布班扎和卡扬扎海拔在1101米至2219米之间;海拔1,640米的Mwaro的三叶草和醋酸松油酯。从捕获物种丰度来看,桔小实蝇以62349只(90.72%)居优势地位,在布琼布拉市捕获数量最多。
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引用次数: 4
Comparative Effects of Coated Compound and Mono-component Proteases on Growth Performance and Nutritional Efficiency in Broiler Diets 包被复合蛋白酶与单组分蛋白酶对肉鸡日粮生长性能和营养效率的影响
Pub Date : 2017-12-28 DOI: 10.17265/2161-6256/2017.06.009
S. Chandrasekar, P. Das, Y. Bashir, M. Karthigan, S. Saravanan
Protease as feed additive is being used in poultry production as a partial replacement for protein sources for cost efficiency and reducing nitrogen excretion. However, diverse proteases may yield different responses under field conditions. A pellet diet study was conducted in Cobb broilers to assess the impact of coated compound (CC) and mono-component (MC) proteases with 5% replacement of digestible amino acids and 0.9% crude protein. Birds fed positive control diet had a better growth than those fed negative control diet, regardless of enzyme supplementation. However, CC protease had shown feed conversion ratio (FCR) like control in a reformulated diet, whereas negative control and MC protease missed to gain the feed conversion. In measures of nutritional efficiency, like energy efficiency, protein efficiency and amino acids efficiency (lysine and methionine), the CC protease proved to be better than MC protease. In terms of European efficiency factor (EEF), control and CC protease elicited a closer response, whereas the other two groups showed a drop. In this study, CC protease allowed partial substitution of digestible amino acids and crude protein, while maintaining feed efficiency and animal performance. It could be concluded that incorporating CC proteases is an efficient choice to maximize the utilization feed material resources and efficiency in animal protein production.
蛋白酶作为饲料添加剂在家禽生产中被用作部分替代蛋白质来源,以提高成本效益和减少氮排泄。然而,不同的蛋白酶在野外条件下可能产生不同的反应。本试验采用颗粒饲粮,以5%的可消化氨基酸和0.9%的粗蛋白质替代包被复合蛋白酶(CC)和单组分蛋白酶(MC)对科布肉仔鸡的影响。无论是否添加酶,饲喂正对照饲粮的雏鸟生长均优于饲喂负对照饲粮的雏鸟。然而,在重新配制的饲料中,CC蛋白酶表现出与对照组相似的饲料转化率,而阴性对照组和MC蛋白酶则未能获得饲料转化率。在能量效率、蛋白质效率和氨基酸(赖氨酸和蛋氨酸)效率等营养效率指标上,CC蛋白酶优于MC蛋白酶。在欧洲效率因子(EEF)方面,对照组和CC蛋白酶引起的反应更接近,而其他两组则有所下降。在本研究中,CC蛋白酶可以部分替代可消化氨基酸和粗蛋白质,同时保持饲料效率和动物生产性能。由此可见,添加CC蛋白酶是提高饲料资源利用率和动物蛋白生产效率的有效选择。
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引用次数: 1
Most Probable Producing Ability of Bali Cows for Calving Interval and Calf Growth Performance 巴厘牛产犊期最可能产犊能力及犊牛生长性能
Pub Date : 2017-12-28 DOI: 10.17265/2161-6256/2017.06.008
A. Supriyantono, T. Widayati, I. Sumpe
Most probable producing ability (MPPA) is the one factor used to compare the performance potential of dam based on the data of their progeny. The purpose of the research was to study the genetic ability of weaning weight, yearling weight and calving interval of Bali cows. The weaning weight data of 296 cows derived from 99 dams, yearling weight data of 245 cows derived from 86 dams and calving interval data of 194 cows from 63 dams were used to estimate repeatability and MPPA of Bali cows in a herd for the three traits. Repeatability of three traits was estimated by analysis of intra-class correlation, and these in turn were used for calculating the estimated MPPA of cows. The results showed that the means of weaning weight of males and females were 95.56 ± 17.25 kg and 87.57 ± 18.45 kg, respectively; means of yearling weight were 143.39 ± 25.78 kg and 136.90 ± 22.01 kg, respectively; and mean calving interval was 391.62 ± 22.59 d. The estimated repeatability of weaning weight, yearling weight and calving interval were 0.006 ± 0.059, 0.022 ± 0.068 and 0.115 ± 0.078, respectively. The best 10 dams in the herd based on the estimates of MPPA for weaning weight, yearling weight and calving interval were not the same for the three traits.
最可能生产能力(MPPA)是根据子代大坝的数据来比较大坝性能潜力的一个因素。本研究旨在研究巴厘牛断奶重、初生重和产犊间隔的遗传能力。利用来自99个坝的296头奶牛的断奶体重数据、来自86个坝的245头奶牛的初生体重数据和来自63个坝的194头奶牛的产犊间隔数据,估算了巴厘岛奶牛在一个群体中这3个性状的重复性和MPPA。通过类内相关性分析估计3个性状的可重复性,进而计算奶牛的MPPA估计值。结果表明:公、母断奶体重均值分别为95.56±17.25 kg和87.57±18.45 kg;平均出生体重分别为143.39±25.78 kg和136.90±22.01 kg;平均产犊间隔为391.62±22.59 d。断奶重、胎重和产犊间隔的重复性分别为0.006±0.059、0.022±0.068和0.115±0.078。根据MPPA估算的断奶重、初生重和产犊间隔在畜群中最佳的10头母猪在3个性状上不尽相同。
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引用次数: 1
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Journal of agricultural science & technology A
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