Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, K. Kawamura
{"title":"Erratum: Experimental simulation of the closed deivertor system for nuclear fusion, (Vol.23, 3&4, pp.22-25)","authors":"Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, K. Kawamura","doi":"10.2978/JSAS.23.E1","DOIUrl":"https://doi.org/10.2978/JSAS.23.E1","url":null,"abstract":"","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74537798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isolation of short, tandem repeat sequences from mayfly Rhithrogena japonica Uéno 1928 has been carried out by probing the multicopy DNA regions using labeled total genomic DNA. Several microsatellites comprised of pyrimidine/purine-biased (Pyr/Pur) repeating units (CT, TCTG, and TCTCTG) and two minisatellites were identified. No locus containing (CA) n repeat has been identified so far by screening with total genomic DNA and (CA) 25 probes. The Pur/Pyr microsatellites may be more abundant than (CA) n microsatellite in the R. japonica genome. Both minisatellite sequences, termed Rja66 family (107bp repeating unit) and Taq I-family (repeating unit about 160bp), have self-complementary regions within the repeating units, thereby conforming to stem-loop structures in single-stranded DNA. As for microsatellite loci, a preliminary analysis on two microsatellites Rja18-1 (CT repeat, FJ827088) and Rja130F (TCTCTG repeat, FJ826512) was done for a local population of R. japonica , showing that they were highly polymorphic. Characterization of genetic structures of local R. japonica populations using Rja18-1 locus, demonstrated significant differences between geographically distant populations indicating an impaired genetic flow between such populations.
{"title":"Isolation of repetitive sequence elements from the genome of a mayfly Rhithrogena japonica (Ephemeroptera: Heptageniidae)","authors":"Pathirana Erandi, Takemon Yasuhiro, Takayanagi Atsushi, M. Eman, Tanida Kazumi, Shimizu Nobuyoshi, Kato Mikio","doi":"10.2978/JSAS.24.1","DOIUrl":"https://doi.org/10.2978/JSAS.24.1","url":null,"abstract":"Isolation of short, tandem repeat sequences from mayfly Rhithrogena japonica Uéno 1928 has been carried out by probing the multicopy DNA regions using labeled total genomic DNA. Several microsatellites comprised of pyrimidine/purine-biased (Pyr/Pur) repeating units (CT, TCTG, and TCTCTG) and two minisatellites were identified. No locus containing (CA) n repeat has been identified so far by screening with total genomic DNA and (CA) 25 probes. The Pur/Pyr microsatellites may be more abundant than (CA) n microsatellite in the R. japonica genome. Both minisatellite sequences, termed Rja66 family (107bp repeating unit) and Taq I-family (repeating unit about 160bp), have self-complementary regions within the repeating units, thereby conforming to stem-loop structures in single-stranded DNA. As for microsatellite loci, a preliminary analysis on two microsatellites Rja18-1 (CT repeat, FJ827088) and Rja130F (TCTCTG repeat, FJ826512) was done for a local population of R. japonica , showing that they were highly polymorphic. Characterization of genetic structures of local R. japonica populations using Rja18-1 locus, demonstrated significant differences between geographically distant populations indicating an impaired genetic flow between such populations.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"309 5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72959272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, T. Iijima, K. Kawamura
{"title":"Experimental simulation of the closed deivertor system for nuclear fusion","authors":"Suguru Tanaka, Hiroyasu Komukai, A. Tonegawa, T. Iijima, K. Kawamura","doi":"10.2978/JSAS.23.22","DOIUrl":"https://doi.org/10.2978/JSAS.23.22","url":null,"abstract":"","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"6 4 1","pages":"22-25"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79515573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, a higher level of medical technology has been required. One such technology is regenerative medicine. In regenerative medicine, much research has been done on stem cells. Likewise, much research has been done on scaffold fabrication techniques. Ink-jet techniques have attracted attention as a method of fabrication of a three-dimensional scaffold. However, scaffolds fabricated by ink-jet techniques require further research. In this study, we have fabricated a highly biocompatible scaffold utilizing an ink-jet technique. The fabrication utilized an electrostatic ink-jet technology called the PELID method. Micro-fiber scaffolds must be suitable for the growth of cells, and film thickness must also be able to be controlled by a few microns. The scaffolds that we have fabricated were designed to satisfy these two requirements, using collagen. We fabricated the scaffolds in spinning mode, which is one of the ink-jet modes. When an applied voltage was high, the diameter of fibers narrowed and film thickness became uniform. In addition, we layered a film of this collagen (scaffold) on MDCK cells, and the cells grew well. We demonstrated that it is possible to fabricate scaffolds with a high biocompatibility by using this method. In the future, we hope to fabricate simple layered body tissue by applying this method.
{"title":"Fabrication of collagen gel fiber films utilizing PELID method","authors":"H. Akiyama, S. Umezu, H. Hashimoto","doi":"10.2978/JSAS.23.14","DOIUrl":"https://doi.org/10.2978/JSAS.23.14","url":null,"abstract":"In recent years, a higher level of medical technology has been required. One such technology is regenerative medicine. In regenerative medicine, much research has been done on stem cells. Likewise, much research has been done on scaffold fabrication techniques. Ink-jet techniques have attracted attention as a method of fabrication of a three-dimensional scaffold. However, scaffolds fabricated by ink-jet techniques require further research. In this study, we have fabricated a highly biocompatible scaffold utilizing an ink-jet technique. The fabrication utilized an electrostatic ink-jet technology called the PELID method. Micro-fiber scaffolds must be suitable for the growth of cells, and film thickness must also be able to be controlled by a few microns. The scaffolds that we have fabricated were designed to satisfy these two requirements, using collagen. We fabricated the scaffolds in spinning mode, which is one of the ink-jet modes. When an applied voltage was high, the diameter of fibers narrowed and film thickness became uniform. In addition, we layered a film of this collagen (scaffold) on MDCK cells, and the cells grew well. We demonstrated that it is possible to fabricate scaffolds with a high biocompatibility by using this method. In the future, we hope to fabricate simple layered body tissue by applying this method.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"1 1","pages":"14-18"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84746995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental study of stabilized supply flow rate on a small bore journal sliding bearing","authors":"Y. Naruse, M. Ochia","doi":"10.2978/JSAS.24.24","DOIUrl":"https://doi.org/10.2978/JSAS.24.24","url":null,"abstract":"","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"31 1","pages":"24-28"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88260055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rei Sato, Kohdai Hatayama, Munkhjargal Munkhbayar, Yuki Matsuura, T. Arakawa, H. Kudo, K. Mitsubayashi
Improvement of the decompression unit “Organic engine” that can generate kinetic energy from the chemical energy of chemicals such as glucose has been proposed and tested. The organic engine was fabricated by separating a cylindrical cell into a top-cell for gas-phase and a bottom-cell for liquid-phase, which separated by glucose oxidase immobilized membrane. Decompression was generated by catalysis of glucose oxidation. However, the concentration of glucose to actuate the system was 100 mmol/L that is too high compared to the human blood glucose concentration to apply it for artificial pancreas-like devices. In order to increase decompression rate, methods to enlarge area of the enzyme membrane per volume of the gas chamber were proposed and evaluated. Decompression rate in modified cell on 25 mmol/L glucose solution was equal to that of in conventional cell (-7.2 Pa・cm/s) on 100 mmol/L glucose solution. As a result, three times higher output was enable to actuate on low glucose concentration close to blood sugar.
{"title":"Improvement of decompression unit in autonomous drug release system for blood sugar control","authors":"Rei Sato, Kohdai Hatayama, Munkhjargal Munkhbayar, Yuki Matsuura, T. Arakawa, H. Kudo, K. Mitsubayashi","doi":"10.2978/JSAS.24.21","DOIUrl":"https://doi.org/10.2978/JSAS.24.21","url":null,"abstract":"Improvement of the decompression unit “Organic engine” that can generate kinetic energy from the chemical energy of chemicals such as glucose has been proposed and tested. The organic engine was fabricated by separating a cylindrical cell into a top-cell for gas-phase and a bottom-cell for liquid-phase, which separated by glucose oxidase immobilized membrane. Decompression was generated by catalysis of glucose oxidation. However, the concentration of glucose to actuate the system was 100 mmol/L that is too high compared to the human blood glucose concentration to apply it for artificial pancreas-like devices. In order to increase decompression rate, methods to enlarge area of the enzyme membrane per volume of the gas chamber were proposed and evaluated. Decompression rate in modified cell on 25 mmol/L glucose solution was equal to that of in conventional cell (-7.2 Pa・cm/s) on 100 mmol/L glucose solution. As a result, three times higher output was enable to actuate on low glucose concentration close to blood sugar.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"3 1","pages":"21-23"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84393503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Kinuta, K. Nakagawa, Yoshiyuki Ichinosawa, Yoshiaki Saita, Masashi Kobayashi, H. Yano, S. Hosaka
In recently, mesh nozzle nebulizer was caused problems of such as nebulizer disorder(Liquid leaking, not in spray). This work used various kinds of metallic nozzles for neburizer. Several liquid, such as skin lotion and solution for cleaning air, were sprayed by these nebulizers. The elution of metals in sprayed solution was analyzed. We show the obtained results with some elucidations.
{"title":"The elusion of metal from neburizer mesh nozzles driven by piezo device","authors":"S. Kinuta, K. Nakagawa, Yoshiyuki Ichinosawa, Yoshiaki Saita, Masashi Kobayashi, H. Yano, S. Hosaka","doi":"10.2978/JSAS.23.1","DOIUrl":"https://doi.org/10.2978/JSAS.23.1","url":null,"abstract":"In recently, mesh nozzle nebulizer was caused problems of such as nebulizer disorder(Liquid leaking, not in spray). This work used various kinds of metallic nozzles for neburizer. Several liquid, such as skin lotion and solution for cleaning air, were sprayed by these nebulizers. The elution of metals in sprayed solution was analyzed. We show the obtained results with some elucidations.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"21 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78482771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ochiai, Shigeomi Hanyuu, Shinichi Harayama, H. Hashimoto
Recently, air bearings are used in high speed and light weight machinery because of their low friction, low environmental load, maintenance-free and so on. On the other hand, the air bearings have low resistant against the outer vibration. Therefore it is important to enhance the stiffness of the bearing. Under the back ground, the authors proposed the high stiffness air bearing which is designed by using the newly developed geometrical optimization method and we conclude the reason for obtaining the high stiffness as generating the negative pressure in the outer vicinity of bearing surface. However, the negative pressure has not been confirmed experimentally yet. In this study, the pressure distribution and load carring capacity of the optimized thrust air bearing are measured under several film thickness conditions by using the high speed experimental setup and then, the reason why the high stiffness is obtained in the optimized bearing was discussed.
{"title":"Measurements of Pressure Distribution and Load Carrying Capacity on Thrust Air Bearing having Optimum Groove Geometry","authors":"M. Ochiai, Shigeomi Hanyuu, Shinichi Harayama, H. Hashimoto","doi":"10.2978/JSAS.23.5","DOIUrl":"https://doi.org/10.2978/JSAS.23.5","url":null,"abstract":"Recently, air bearings are used in high speed and light weight machinery because of their low friction, low environmental load, maintenance-free and so on. On the other hand, the air bearings have low resistant against the outer vibration. Therefore it is important to enhance the stiffness of the bearing. Under the back ground, the authors proposed the high stiffness air bearing which is designed by using the newly developed geometrical optimization method and we conclude the reason for obtaining the high stiffness as generating the negative pressure in the outer vicinity of bearing surface. However, the negative pressure has not been confirmed experimentally yet. In this study, the pressure distribution and load carring capacity of the optimized thrust air bearing are measured under several film thickness conditions by using the high speed experimental setup and then, the reason why the high stiffness is obtained in the optimized bearing was discussed.","PeriodicalId":14991,"journal":{"name":"Journal of Advanced Science","volume":"28 1","pages":"5-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86200004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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