Pub Date : 2021-05-30DOI: 10.37231/JAB.2021.12.1.219
R. Widowati, Yasinta Marescoty Rosana, Vivi Silawati, Albiruni Raushanfikri
This study aims to examine the effect of honey from Apis cerana bee colonies on nutritional status in children aged two to five years old in the working area of Mok Community Health Center, Manggarai Regency, East Nusa Tenggara - Indonesia. The study was conducted in May - June 2018, with quasi-experiment design with pre and post test in one group. The sample in this study amounted to 35 children under five years old, with malnutritional status. Nutritional status is determined by the weight-for-age. The used honey was honey from the A. cerana bee colonies harvested in Manggarai Regency. Honey was consumed by children as much as 5 mL, twice a day, morning and evening, for 8 weeks. Nutritional status measurements were taken at 0 weeks, 4 weeks and 8 weeks before and after honey consumed regularly. Post-Hoc data analysis using the Wilcoxon Signed Rank Test. The results showed a significant increase in children's nutritional status at week 4 and week 8. The percentage of children with malnutritional status after being given honey A. cerana regularly for 4 weeks fell to 57% and good nutrition increased to 43%. Meanwhile, after 8 weeks of honey, the malnutritional status has decreased to 31%, and vice versa 69% of children have increased their nutritional status to good nutrition. The results of a comparative test of increasing nutritional status between 4 and 8 weeks showed that there was a significant difference between improving the nutritional status of children under five years old who consumed honey for 4 weeks and for 8 weeks. The research shows the length of time consumed A. cerana honey regularly in children aged two to five years old, will have a positive effect to the nutritional status from poor to good nutritional status.
{"title":"Honey and Children: The Effect of Honey from Apis cerana Bees on Children’ Nutritional Status in East Nusa Tenggara - Indonesia","authors":"R. Widowati, Yasinta Marescoty Rosana, Vivi Silawati, Albiruni Raushanfikri","doi":"10.37231/JAB.2021.12.1.219","DOIUrl":"https://doi.org/10.37231/JAB.2021.12.1.219","url":null,"abstract":"This study aims to examine the effect of honey from Apis cerana bee colonies on nutritional status in children aged two to five years old in the working area of Mok Community Health Center, Manggarai Regency, East Nusa Tenggara - Indonesia. The study was conducted in May - June 2018, with quasi-experiment design with pre and post test in one group. The sample in this study amounted to 35 children under five years old, with malnutritional status. Nutritional status is determined by the weight-for-age. The used honey was honey from the A. cerana bee colonies harvested in Manggarai Regency. Honey was consumed by children as much as 5 mL, twice a day, morning and evening, for 8 weeks. Nutritional status measurements were taken at 0 weeks, 4 weeks and 8 weeks before and after honey consumed regularly. Post-Hoc data analysis using the Wilcoxon Signed Rank Test. The results showed a significant increase in children's nutritional status at week 4 and week 8. The percentage of children with malnutritional status after being given honey A. cerana regularly for 4 weeks fell to 57% and good nutrition increased to 43%. Meanwhile, after 8 weeks of honey, the malnutritional status has decreased to 31%, and vice versa 69% of children have increased their nutritional status to good nutrition. The results of a comparative test of increasing nutritional status between 4 and 8 weeks showed that there was a significant difference between improving the nutritional status of children under five years old who consumed honey for 4 weeks and for 8 weeks. The research shows the length of time consumed A. cerana honey regularly in children aged two to five years old, will have a positive effect to the nutritional status from poor to good nutritional status.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"293 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83437246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-09DOI: 10.37231/JAB.2021.12.1.242
T. Usman, S. M. Sir, S. Haladu, A. Kari
In poultry breeding program the success of artificial insemination (AI) is highly influenced by the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.
{"title":"Assessment of Semen Characteristics Among Three Phenotypes of Chicken Raised in Akko, Gombe State of Nigeria","authors":"T. Usman, S. M. Sir, S. Haladu, A. Kari","doi":"10.37231/JAB.2021.12.1.242","DOIUrl":"https://doi.org/10.37231/JAB.2021.12.1.242","url":null,"abstract":"In poultry breeding program the success of artificial insemination (AI) is highly influenced by the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87052880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-09DOI: 10.37231/JAB.2021.12.1.243
Nurul Sherina Muzaini, Nurulhuda Mohd Jailani, T. Hamid
Protease is an important industrial enzyme and salt tolerant protease in which has desirable properties that could enhance its uses not only in industries, but also in agriculture and environmental. Marine organisms usually harbour halophilic microorganisms which produce salt tolerant protease. In this study, salt tolerant protease producing bacteria from marine sponge were isolated and screened on skim milk marine agar supplemented with different NaCl concentrations (1.5 % w/v). Out of 11 isolates, 8 isolates (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 and S2-6) showed clearing zones with ability to digest casein on the skim milk agar. Morphologically, these strains are gram negative bacilli which grow in yellow colonies and were found to be catalase-positive but oxidase-negative. They are also non-lactose fermenter that produce gelatinase but not α-amylase. The ribosomal 16S rRNA sequencing was used to identify each isolate (Acc. number of S1-1 for MT645770, S1-2 for MT645 771, S1-3 for MT645 772, S2-1 MT645 773, S2-3 MT645 774, S2-4 MT645 775, S2-5 MT645 776 and S2-6 MT645 7767). The 16S rRNA sequences showed that these isolates were highly similar to Stenotrophomonas maltophilia (S2-6, 99.87%) and to a related strain Pseudomonas hibiscicola (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 (97.16-99.9 %). Further proteolytic studies were carried out using skim milk agar with 1.0 %, 2.0 %, 3.0 % and 4.o % (w/v) of NaCl concentrations. All isolates were able to hydrolyze casein which produced clear zones surrounding each colony at 1.0 % and 2.0 % (w/v) salt. However, only isolate S1-5 and S2-6 showed proteolytic activities at 3.0 % (w/v) salt but none of them at 4.0 % (w/v). The ability of these isolates to produce protease which active at higher salt may indicate their potential to be the sources for enzyme with useful properties.
{"title":"The Characterization of Stenotrophomonas maltophilia Isolated from Marine Sponge Producing Salt Tolerant Proteases","authors":"Nurul Sherina Muzaini, Nurulhuda Mohd Jailani, T. Hamid","doi":"10.37231/JAB.2021.12.1.243","DOIUrl":"https://doi.org/10.37231/JAB.2021.12.1.243","url":null,"abstract":"Protease is an important industrial enzyme and salt tolerant protease in which has desirable properties that could enhance its uses not only in industries, but also in agriculture and environmental. Marine organisms usually harbour halophilic microorganisms which produce salt tolerant protease. In this study, salt tolerant protease producing bacteria from marine sponge were isolated and screened on skim milk marine agar supplemented with different NaCl concentrations (1.5 % w/v). Out of 11 isolates, 8 isolates (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 and S2-6) showed clearing zones with ability to digest casein on the skim milk agar. Morphologically, these strains are gram negative bacilli which grow in yellow colonies and were found to be catalase-positive but oxidase-negative. They are also non-lactose fermenter that produce gelatinase but not α-amylase. The ribosomal 16S rRNA sequencing was used to identify each isolate (Acc. number of S1-1 for MT645770, S1-2 for MT645 771, S1-3 for MT645 772, S2-1 MT645 773, S2-3 MT645 774, S2-4 MT645 775, S2-5 MT645 776 and S2-6 MT645 7767). The 16S rRNA sequences showed that these isolates were highly similar to Stenotrophomonas maltophilia (S2-6, 99.87%) and to a related strain Pseudomonas hibiscicola (S1-1, S1-2, S1-3, S2-1, S2-3, S2-4, S2-5 (97.16-99.9 %). Further proteolytic studies were carried out using skim milk agar with 1.0 %, 2.0 %, 3.0 % and 4.o % (w/v) of NaCl concentrations. All isolates were able to hydrolyze casein which produced clear zones surrounding each colony at 1.0 % and 2.0 % (w/v) salt. However, only isolate S1-5 and S2-6 showed proteolytic activities at 3.0 % (w/v) salt but none of them at 4.0 % (w/v). The ability of these isolates to produce protease which active at higher salt may indicate their potential to be the sources for enzyme with useful properties.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84412629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.37231/jab.2021.12.2.248
Wan Amalina Wan Mamat, Syed Ahmad Tajudin Tuan Johari, Muhammad Yusran Abdul Aziz, Ahmad Syibli Othman, Abdul Manaf Ali
Catunaregam tomentosa (Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family, and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory, antispasmodic, antidysenteric, antifertility and immunomodulatory. In this study, the leaves were extracted using dichloromethane, ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method while total flavonoid was determined by the aluminium chloride calorimetric method. Meanwhile, its antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content. Interestingly, ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.07 ± 0.51µg/mL.
Catunaregam tomentosa (Blume ex DC) Tirveng在泰国通常被称为Khet，在马来西亚被称为Bisa Ular或Badang。这种树广泛分布在泰国东北部地区，而在马来西亚，这种树通常生长在登嘉楼的开阔海滨地区。该植物属于茜草科，具有抗炎、抗痉挛、抗痢疾、抗生育和免疫调节等药理作用。本研究以二氯甲烷、乙酸乙酯和乙醇为萃取剂。总酚用福林-乔卡多法测定，总黄酮用氯化铝量热法测定。同时，采用2,2-二苯基-1-苦味酰肼(DPPH)法测定其抗氧化活性。发现乙醇提取物的酚类和类黄酮含量最高。有趣的是，乙醇提取物也显示出较强的DPPH清除活性，IC50为20.07±0.51µg/mL。
{"title":"Evaluation of the DPPH Radical Scavenging Activity, Total Phenolic Content and Total Flavanoid Content of Different Solvent Extracts of Catunaregam tomentosa (Blume ex DC) Tirveng Leaves","authors":"Wan Amalina Wan Mamat, Syed Ahmad Tajudin Tuan Johari, Muhammad Yusran Abdul Aziz, Ahmad Syibli Othman, Abdul Manaf Ali","doi":"10.37231/jab.2021.12.2.248","DOIUrl":"https://doi.org/10.37231/jab.2021.12.2.248","url":null,"abstract":"Catunaregam tomentosa (Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family, and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory, antispasmodic, antidysenteric, antifertility and immunomodulatory. In this study, the leaves were extracted using dichloromethane, ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method while total flavonoid was determined by the aluminium chloride calorimetric method. Meanwhile, its antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content. Interestingly, ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.07 ± 0.51µg/mL.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84122827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-31DOI: 10.37231/jab.2020.11.1s.235
Anis Adilah Mustafa, N. A. Zulkifly, T. Abdullah, N. Alias
The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia, which were in Labis, Johor (L3), Dusun Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30°C and 37°C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall, strain L3 (from Labis, Johor) exhibit promising rate of Pi released in the media at 30°C and 37°C, with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL, respectively. The pH of the cultures was also measured, where it shows that strains grown in cultures at 37°C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30°C. All around, L3 strains has the lowest value of pH when cultured at 30°C and 37°C, with the pH value of 3.62 and 2.37, respectively. From the result obtained, the lower pH indicates the process of phytic acid degradation take place by the phytase in producing inorganic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs, further analysis of temperature optimization using 55°C and even 60°C should be carried out. In the future, biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.
本研究的主要目的是寻找不同产植酸酶潜力菌株的最佳生长曲线和产植酸酶的最佳温度。所使用的四种菌株最初是从马来西亚的温泉中分离出来的，分别是柔佛州拉比斯(L3)、雪兰莪州Dusun Tua (RT)、吉打州乌鲁勒贡(A)和沙巴拉瑙(B9)。采用营养琼脂(NA)和改良植酸酶筛选培养基(PSM)液体培养基进行培养富集，并采用摇瓶规模分批培养法进行优化。在不同温度下(30°C和37°C)定量测定菌株生长和酶活性。根据植酸酶与其底物(植酸钠)的反应测定酶活性，以植酸酶活性单位(U/ mL)表示。总体而言，菌株L3(来自柔佛Labis, Johor)在30°C和37°C的培养基中表现出良好的Pi释放率，最佳植酸酶活性值分别为0.2047 U/mL和0.2195 U/mL。还测量了培养物的pH值，结果表明，与在30°C培养的菌株相比，在37°C培养的菌株产生更高的植酸酶活性，pH值读数较低。从各方面来看，L3菌株在30℃和37℃培养时pH值最低，分别为3.62和2.37。从得到的结果来看，较低的pH值表明由于有机酸的积累，植酸酶在产生无机磷酸盐(Pi)时发生了植酸降解过程。由于这些菌株最初是从温泉中提取的，因此需要在55°C甚至60°C下进行进一步的温度优化分析。今后还可开展生化研究和分子鉴定，对菌株进行分子鉴定。
{"title":"Comparison on Effects of Temperature on Different Strains of Phytase Producing Bacteria Isolated from Malaysia’s Hot Spring","authors":"Anis Adilah Mustafa, N. A. Zulkifly, T. Abdullah, N. Alias","doi":"10.37231/jab.2020.11.1s.235","DOIUrl":"https://doi.org/10.37231/jab.2020.11.1s.235","url":null,"abstract":"The main purpose of this research was to find the best growth curve for bacterial growth and the optimum temperature for the production of phytase from different potential phytase producing bacterial strains. A total of four strains used were originally isolated from hot springs in Malaysia, which were in Labis, Johor (L3), Dusun Tua, Selangor (RT), Ulu Legong, Kedah (A) and Ranau, Sabah (B9). Nutrient Agar (NA) and modified Phytase Screening Medium (PSM) liquid media were used for the culture enrichment while optimisation was carried out through batch culture method using a shake-flask scale. Strains growth and enzyme activity were quantitatively measured at different temperatures at (30°C and 37°C) values. Enzyme activity was determined according to the reaction of the phytase with its substrate (sodium phytate) and expressed in units of phytase activity (U/ mL). As for the overall, strain L3 (from Labis, Johor) exhibit promising rate of Pi released in the media at 30°C and 37°C, with optimum phytase activity values of 0.2047 U/mL and 0.2195 U/mL, respectively. The pH of the cultures was also measured, where it shows that strains grown in cultures at 37°C produced a higher phytase activity and resulting a lower reading of pH compared when grown at 30°C. All around, L3 strains has the lowest value of pH when cultured at 30°C and 37°C, with the pH value of 3.62 and 2.37, respectively. From the result obtained, the lower pH indicates the process of phytic acid degradation take place by the phytase in producing inorganic phosphate (Pi) due to the accumulation of organic acid. Since these bacterial strains were originally taken from Hot Springs, further analysis of temperature optimization using 55°C and even 60°C should be carried out. In the future, biochemical research and molecular identification may also be carried out to identify molecular identity in the strains.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89044619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-31DOI: 10.37231/jab.2020.11.1s.239
Salmah Mohamed, Siti Nur Shafiqa Abdullah, Nur Syafiqah Musa, Norhayati Ngah
A study was conducted to attract insects using different colours of sticky trap in pumpkin (Cucurbita moschata) field. Sticky trap was chosen as it is one of a method to estimate the insect population density in field as it requires a low cost and less skilled labour. Four different colours of sticky traps (i.e. red, white, blue and yellow) were used to determine the insect population abundance at the pumpkin field. All the traps were installed at the height of 100 cm at a random of 1 ha of pumpkin plot with five replicates for each colour and the insect samples were collected weekly for three months (October-December 2019). Overall, a total of 13,052 insects were collected throughout 11 weeks of sampling. The results showed that the percentage of insect population abundance recorded the highest was on week eight (15.01%) whilst the least abundance of insects trapped was on week one (2.28%). The most attractive colour to attract insects was significantly the yellow colour (44.34%) followed by blue (20.12%) and white (19.15%) whilst the lowest insect trapped was on red colour (16.40%). A total of nine insect orders recorded were; Diptera, Hemiptera, Coleoptera, Hymenoptera, Lepidoptera, Orthoptera, Blattodea, Isoptera and others. Diptera was the most abundant of insect’s order trapped in the yellow trap with 3427 individuals and followed by Hemiptera order (1022 individuals). Whilst the Isoptera order was the least number of insects caught on a red colour trap with only one individual. In conclusion, our findings showed that the yellow sticky trap colour is the most attractive to attract insects of C. moschata compared to other colours. Therefore, this study could provide essential knowledge that may be useful for the future ecological survey of insects of C. moschata.
{"title":"Population Abundance of Insect Trapped on Different Colours of Sticky Trap in Pumpkin (Cucurbita moschata) Field","authors":"Salmah Mohamed, Siti Nur Shafiqa Abdullah, Nur Syafiqah Musa, Norhayati Ngah","doi":"10.37231/jab.2020.11.1s.239","DOIUrl":"https://doi.org/10.37231/jab.2020.11.1s.239","url":null,"abstract":"A study was conducted to attract insects using different colours of sticky trap in pumpkin (Cucurbita moschata) field. Sticky trap was chosen as it is one of a method to estimate the insect population density in field as it requires a low cost and less skilled labour. Four different colours of sticky traps (i.e. red, white, blue and yellow) were used to determine the insect population abundance at the pumpkin field. All the traps were installed at the height of 100 cm at a random of 1 ha of pumpkin plot with five replicates for each colour and the insect samples were collected weekly for three months (October-December 2019). Overall, a total of 13,052 insects were collected throughout 11 weeks of sampling. The results showed that the percentage of insect population abundance recorded the highest was on week eight (15.01%) whilst the least abundance of insects trapped was on week one (2.28%). The most attractive colour to attract insects was significantly the yellow colour (44.34%) followed by blue (20.12%) and white (19.15%) whilst the lowest insect trapped was on red colour (16.40%). A total of nine insect orders recorded were; Diptera, Hemiptera, Coleoptera, Hymenoptera, Lepidoptera, Orthoptera, Blattodea, Isoptera and others. Diptera was the most abundant of insect’s order trapped in the yellow trap with 3427 individuals and followed by Hemiptera order (1022 individuals). Whilst the Isoptera order was the least number of insects caught on a red colour trap with only one individual. In conclusion, our findings showed that the yellow sticky trap colour is the most attractive to attract insects of C. moschata compared to other colours. Therefore, this study could provide essential knowledge that may be useful for the future ecological survey of insects of C. moschata.","PeriodicalId":14995,"journal":{"name":"Journal Of Agrobiotechnology","volume":"170 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91464654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-09-06DOI: 10.37231/jab.2020.11.2.221
Md. Amirul Alam, Noor Elyana Ahmad Ismail
The bitter taste of Momordica charantia due to its high level of calcium content limits people from consuming it, although it has enough various beneficial nutrients. Parental line evaluation is prerequisite for any desired improvement activities through breeding. In this regard M. charantia parental lines were evaluated based on bitterness (high Ca content) and morpho-nutritional attributes to augment additional value and opportunity to be commercialized. Field and laboratory experiments were carried out for three open-pollinated M. charantia varieties to screen low bitterness parental lines based on low calcium contents and other morpho-nutritional qualities, targeting to generalize this important vegetable crops for all types of consumers from child to older age. From the overall results of the study, Variety 2 showed better quality for numbers of fruits per plant, fruit weight (g), moisture content (%), chlorophyll content (mmol/m²), stomatal conductance (mmol/m²s) and nutrients content such as calcium (Ca), nitrogen (N), magnesium (Mg) and iron (Fe). Variety 3 had good quality for morphological parameters like plant height (cm), numbers of leaves per plant, leaf area (cm²), fruit length (cm), phosphorus (P), potassium (K), aluminium (Al) and copper (Cu). Variety 1 had the highest values only for number of branches per plant and amount of sodium (Na) content. After that, Variety 2 was identified for having lowest calcium (Ca) contents compared to the other two varieties. Thus, Variety 2 was selected as better parents to hold good promise for hybridization based breeding programs for varietal improvement in obtaining low bitterness bitter gourd but rich in other nutrient contents.
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