Pub Date : 2024-08-03DOI: 10.1177/08839115241268608
Anh Thi Ngoc Doan, Natsuki Kojima, Kazuo Sakurai
This paper explores the development and characterization of epichlorohydrin-crosslinked β-cyclodextrin nanoparticles (βCDNPs) for drug delivery, focusing on their interaction with hydrophobic drugs and biocompatibility. We synthesized βCDNPs using β-cyclodextrin (βCD) and epichlorohydrin (ECH). Our study assessed the biocompatibility and safety of βCDNPs, particularly in avoiding nephrotoxicity commonly associated with βCD, by examining their interaction with cholesterol and conducting survival analyses in mice at various dosing concentrations. Additionally, we highlight the advantages of using diffusion-ordered NMR spectroscopy (DOSY) to determine the enhanced binding constants of βCDNPs with hydrophobic compounds, in comparison with the solubility method, Job plot analysis, and isothermal titration calorimetry (ITC). We demonstrated the enhanced binding capacity of βCDNPs compared to βCD alone and established the polymerization of βCD as a significant factor in this enhancement. Our findings suggest that βCDNPs show promise as drug delivery systems due to their improved solubility, stability, and safety profiles.
{"title":"Reduced nephrotoxicity of epichlorohydrin-crosslinked β-cyclodextrin nanoparticles (βCDNPs) and its enhanced binding with hydrophobic compounds","authors":"Anh Thi Ngoc Doan, Natsuki Kojima, Kazuo Sakurai","doi":"10.1177/08839115241268608","DOIUrl":"https://doi.org/10.1177/08839115241268608","url":null,"abstract":"This paper explores the development and characterization of epichlorohydrin-crosslinked β-cyclodextrin nanoparticles (βCDNPs) for drug delivery, focusing on their interaction with hydrophobic drugs and biocompatibility. We synthesized βCDNPs using β-cyclodextrin (βCD) and epichlorohydrin (ECH). Our study assessed the biocompatibility and safety of βCDNPs, particularly in avoiding nephrotoxicity commonly associated with βCD, by examining their interaction with cholesterol and conducting survival analyses in mice at various dosing concentrations. Additionally, we highlight the advantages of using diffusion-ordered NMR spectroscopy (DOSY) to determine the enhanced binding constants of βCDNPs with hydrophobic compounds, in comparison with the solubility method, Job plot analysis, and isothermal titration calorimetry (ITC). We demonstrated the enhanced binding capacity of βCDNPs compared to βCD alone and established the polymerization of βCD as a significant factor in this enhancement. Our findings suggest that βCDNPs show promise as drug delivery systems due to their improved solubility, stability, and safety profiles.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141935750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-27DOI: 10.1177/08839115241262036
Ting Bai, Yuemei Han, Chen Qin, Di Hu, Quankui Lin
Cataract is the leading cause of the blindness worldwide. Natural lens removal followed with intraocular lens (IOL) implantation is the main clinical treatment for cataract. However, the shape and the optical power of current IOLs were fixed, which were not favorable for patients, especially for children with congenital cataracts. An injectable IOL is an immerging replacement for a regular IOL due to the accommodation of external packing shapes. In this study, we developed a rapid, in situ gelation of an injectable photo-crosslinked hydrogel as an injectable IOL material. In this investigation, injectable hydrogel (G/D hydrogel) was fabricated from Gelatin methacrylate (GelMA) and N- (3, 4-dihydroxyphenylidene ethyl) methacrylamide (DMA) via photo-crosslinking for injectable IOL applications. Different preparation parameters such as the concentration, proportion, light intensity, and curing time were optimized based on the gelation time, swelling ratio, and mechanical properties of the produced G/D hydrogels. The results of in vitro cellular experiments showed that the G/D hydrogel had good and stable clearance of lens epithelial cells. The hydrogel was implanted into the eyes of the young rabbits for 1 month, and the results also showed that injectable G/D hydrogel can obtain good intraocular implants and have inhibitory effects on posterior capsular opacification. Thus such photo-crosslinked G/D hydrogel can serve as an injectable IOL application in cataract surgery.
{"title":"Photo-crosslinked hydrogel as injectable intraocular lens for cataract surgery implantation","authors":"Ting Bai, Yuemei Han, Chen Qin, Di Hu, Quankui Lin","doi":"10.1177/08839115241262036","DOIUrl":"https://doi.org/10.1177/08839115241262036","url":null,"abstract":"Cataract is the leading cause of the blindness worldwide. Natural lens removal followed with intraocular lens (IOL) implantation is the main clinical treatment for cataract. However, the shape and the optical power of current IOLs were fixed, which were not favorable for patients, especially for children with congenital cataracts. An injectable IOL is an immerging replacement for a regular IOL due to the accommodation of external packing shapes. In this study, we developed a rapid, in situ gelation of an injectable photo-crosslinked hydrogel as an injectable IOL material. In this investigation, injectable hydrogel (G/D hydrogel) was fabricated from Gelatin methacrylate (GelMA) and N- (3, 4-dihydroxyphenylidene ethyl) methacrylamide (DMA) via photo-crosslinking for injectable IOL applications. Different preparation parameters such as the concentration, proportion, light intensity, and curing time were optimized based on the gelation time, swelling ratio, and mechanical properties of the produced G/D hydrogels. The results of in vitro cellular experiments showed that the G/D hydrogel had good and stable clearance of lens epithelial cells. The hydrogel was implanted into the eyes of the young rabbits for 1 month, and the results also showed that injectable G/D hydrogel can obtain good intraocular implants and have inhibitory effects on posterior capsular opacification. Thus such photo-crosslinked G/D hydrogel can serve as an injectable IOL application in cataract surgery.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":2.1,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141797438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-22DOI: 10.1177/08839115241258150
Feng Xu, GuiBin Su
In the current research, the nanofibrous neural channels were produced by electrospinning of PCL/Gelatin solution loaded with Calendula officinalis extract. The produced scaffolds properties were studied using various in vitro characterization methods. Then, the conduits’ healing potential was investigated in a rat model of PNS injury. In vitro results showed that scaffolds were not toxic and quenched DPPH free radicals. In addition, the extract-loaded conduits exhibited significant anti-inflammatory activity. In vivo study showed that the regular treadmill exercise significantly improved the healing efficacy of the Calendula officinalis-delivering conduits by upregulating BDNF and GFAP proteins.
{"title":"Calendula officinalis extract-loaded conduits improved sciatic nerve injury repair through upregulation of BDNF and GFAP","authors":"Feng Xu, GuiBin Su","doi":"10.1177/08839115241258150","DOIUrl":"https://doi.org/10.1177/08839115241258150","url":null,"abstract":"In the current research, the nanofibrous neural channels were produced by electrospinning of PCL/Gelatin solution loaded with Calendula officinalis extract. The produced scaffolds properties were studied using various in vitro characterization methods. Then, the conduits’ healing potential was investigated in a rat model of PNS injury. In vitro results showed that scaffolds were not toxic and quenched DPPH free radicals. In addition, the extract-loaded conduits exhibited significant anti-inflammatory activity. In vivo study showed that the regular treadmill exercise significantly improved the healing efficacy of the Calendula officinalis-delivering conduits by upregulating BDNF and GFAP proteins.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-21DOI: 10.1177/08839115241261421
Antoniyar Arockia Raj, Subramanian Sundaramoorthy
This study presents the synthesis and characterization of an amine-functionalized cellulose nanogel (AFCNG) for potential biomedical applications. AFCNG is synthesized using a combination of acid hydrolysis, TEMPO oxidation, and EDC/NHS-mediated coupling via amide linkage. Biodegradable AFCNG is formulated with a mean size of 74 nm through a regeneration process. The characterization of AFCNG is performed using various techniques such as FTIR spectroscopy, XRD, XPS, Zeta potential, DLS, HRSEM, and TGA. The result reveals that the anionic AFCNG is amorphous, highly stable in colloidal form, and has a higher degradation temperature and water absorption capacity. Biodegradation of AFCNG is also assessed by incubating it with lysozyme for 5 weeks, which results in a degradation level of 83%. There is negligible hemolytic activity and less cytotoxicity toward 3T3 cells induced by the AFCNG. The model drug, curcumin, is effectively loaded on AFCNG, which is highly released at an acidic pH by using the Franz diffusion method. The anionic ligand-based CAFCNG is effectively conjugated with the cationic folic acid receptors on the surface of B16-F10 cell lines through receptor-mediated endocytosis. The resulting CAFCNG shows notable cytotoxicity to B16-F10 cells. The results imply that CAFCNG is a suitable candidate for the treatment of skin cancers by using an effective transdermal drug carrier.
{"title":"Synthesis and characterization of amine functionalized cellulose nanogel for transdermal drug delivery","authors":"Antoniyar Arockia Raj, Subramanian Sundaramoorthy","doi":"10.1177/08839115241261421","DOIUrl":"https://doi.org/10.1177/08839115241261421","url":null,"abstract":"This study presents the synthesis and characterization of an amine-functionalized cellulose nanogel (AFCNG) for potential biomedical applications. AFCNG is synthesized using a combination of acid hydrolysis, TEMPO oxidation, and EDC/NHS-mediated coupling via amide linkage. Biodegradable AFCNG is formulated with a mean size of 74 nm through a regeneration process. The characterization of AFCNG is performed using various techniques such as FTIR spectroscopy, XRD, XPS, Zeta potential, DLS, HRSEM, and TGA. The result reveals that the anionic AFCNG is amorphous, highly stable in colloidal form, and has a higher degradation temperature and water absorption capacity. Biodegradation of AFCNG is also assessed by incubating it with lysozyme for 5 weeks, which results in a degradation level of 83%. There is negligible hemolytic activity and less cytotoxicity toward 3T3 cells induced by the AFCNG. The model drug, curcumin, is effectively loaded on AFCNG, which is highly released at an acidic pH by using the Franz diffusion method. The anionic ligand-based CAFCNG is effectively conjugated with the cationic folic acid receptors on the surface of B16-F10 cell lines through receptor-mediated endocytosis. The resulting CAFCNG shows notable cytotoxicity to B16-F10 cells. The results imply that CAFCNG is a suitable candidate for the treatment of skin cancers by using an effective transdermal drug carrier.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-21DOI: 10.1177/08839115241260096
Kathryn Uhrich
{"title":"Tribute","authors":"Kathryn Uhrich","doi":"10.1177/08839115241260096","DOIUrl":"https://doi.org/10.1177/08839115241260096","url":null,"abstract":"","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141549318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-15DOI: 10.1177/08839115241260060
Reza Aslani, Motahareh Mirzadeh, Seyed Ali Poursamar, Abbas Ali Rabiei, Mohsen Setayeshmehr
Extracellular matrix (ECM) is widely used for clinical purposes in tissue engineering (TE). Since ECM does not have favorable mechanical properties, its use has been limited. Therefore, it is helpful to use synthetic polymers such as polycaprolactone (PCL) to improve the mechanical properties of ECM-based scaffolds. PCL scaffolds were prepared via the three-dimensional (3D) printing method. Cartilaginous ECM was obtained from bovine femur, and then it was decellularized and solubilized. PCL scaffolds were functionalized using 1,6-hexanediamine; consequently, the scaffolds were coated with solubilized decellularized ECM (SDECM) using two types of crosslinkers; namely, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) and genipin. Genipin-crosslinked SDECM-coated (PCL/ECM-Gen) scaffolds and EDC/NHS-crosslinked SDECM-coated (PCL/ECM-EN) scaffolds were characterized by different tests. Following loading the curcumin (Cur) on the scaffolds, the Cur release rate was investigated. Finally, human chondrocyte cells were cultured on the scaffolds to explore cell viability, cell attachment, and histological studies. Following functionalization via amine groups, 5% SDECM was used to coat the scaffolds, and this increased the wettability and cell viability of the PCL. Genipin crosslinked and coated the SDECM more efficiently compared to the EDC/NHS and led to lower porosity, water absorption capacity, degradation rate, higher cell proliferation, and cell attachment. Genipin-crosslinked Cur-loaded (PCL/ECM-Gen + Cur) scaffolds showed higher cell viability but lower antibacterial activity compared to EDC/NHS-crosslinked Cur-loaded (PCL/ECM-EN + Cur) scaffolds, which may indicate EDC/NHS-induced cytotoxicity. This study elucidates the value of PCL/ECM-Gen + Cur scaffolds as highly biocompatible scaffolds that can be considered a promising tool for cartilage TE applications.
{"title":"Extracellular matrix coated three-dimensional-printed polycaprolactone scaffolds containing curcumin for cartilage tissue engineering applications","authors":"Reza Aslani, Motahareh Mirzadeh, Seyed Ali Poursamar, Abbas Ali Rabiei, Mohsen Setayeshmehr","doi":"10.1177/08839115241260060","DOIUrl":"https://doi.org/10.1177/08839115241260060","url":null,"abstract":"Extracellular matrix (ECM) is widely used for clinical purposes in tissue engineering (TE). Since ECM does not have favorable mechanical properties, its use has been limited. Therefore, it is helpful to use synthetic polymers such as polycaprolactone (PCL) to improve the mechanical properties of ECM-based scaffolds. PCL scaffolds were prepared via the three-dimensional (3D) printing method. Cartilaginous ECM was obtained from bovine femur, and then it was decellularized and solubilized. PCL scaffolds were functionalized using 1,6-hexanediamine; consequently, the scaffolds were coated with solubilized decellularized ECM (SDECM) using two types of crosslinkers; namely, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) and genipin. Genipin-crosslinked SDECM-coated (PCL/ECM-Gen) scaffolds and EDC/NHS-crosslinked SDECM-coated (PCL/ECM-EN) scaffolds were characterized by different tests. Following loading the curcumin (Cur) on the scaffolds, the Cur release rate was investigated. Finally, human chondrocyte cells were cultured on the scaffolds to explore cell viability, cell attachment, and histological studies. Following functionalization via amine groups, 5% SDECM was used to coat the scaffolds, and this increased the wettability and cell viability of the PCL. Genipin crosslinked and coated the SDECM more efficiently compared to the EDC/NHS and led to lower porosity, water absorption capacity, degradation rate, higher cell proliferation, and cell attachment. Genipin-crosslinked Cur-loaded (PCL/ECM-Gen + Cur) scaffolds showed higher cell viability but lower antibacterial activity compared to EDC/NHS-crosslinked Cur-loaded (PCL/ECM-EN + Cur) scaffolds, which may indicate EDC/NHS-induced cytotoxicity. This study elucidates the value of PCL/ECM-Gen + Cur scaffolds as highly biocompatible scaffolds that can be considered a promising tool for cartilage TE applications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141337438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-31DOI: 10.1177/08839115241253560
Mohd Ibnu Haikal Ahmad Sohaimy, Muhammad Amir Amzar Ismail, Azwani Sofia Ahmad Khiar, Norizah Mhd Sarbon, Nora Salina Md Salim, Hanis Nadia Yahya, Mohd Ikmar Nizam Mohamad Isa
A wound dressing is important to ensure an efficient healing process while protecting the wound area. This research study combined 2-hydroxyethyl cellulose (2HEC)—an etherified cellulose derivative with salicylic acid (SA) to develop a single layer and investigate the 2HEC viability as wound dressing material. Nine different samples with different compositions of SA, from 5 wt.% to 40 wt.% (with an interval of 5 wt.%) and one control sample without adding SA were prepared via the solution casting method. The 2HEC-SA films were studied regarding the effects of SA composition on antimicrobial properties ( Staphylococcus aureus) via the well-diffusion method. Additionally, degradability, mechanical properties, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) of 2HEC-SA films have also been tested. The strongest antimicrobial effect of 2HEC-SA film was obtained at 40 wt.% with a 16 mm inhibition zone diameter. There was a noticeable decreasing weight loss pattern in the degradation test and the tensile strength of 2HEC-SA film when the composition of salicylic acid is increased. 2HEC-SA film changes phases from amorphous to crystalline starting at 25 wt.% of salicylic acid as seen through XRD, while FTIR shows that complexation of 2HEC and salicylic acid occurred at 1050 cm−1.
{"title":"Development of cellulose-based films containing salicylic acid for wound dressing applications: Fabrication, properties and in vitro assessment","authors":"Mohd Ibnu Haikal Ahmad Sohaimy, Muhammad Amir Amzar Ismail, Azwani Sofia Ahmad Khiar, Norizah Mhd Sarbon, Nora Salina Md Salim, Hanis Nadia Yahya, Mohd Ikmar Nizam Mohamad Isa","doi":"10.1177/08839115241253560","DOIUrl":"https://doi.org/10.1177/08839115241253560","url":null,"abstract":"A wound dressing is important to ensure an efficient healing process while protecting the wound area. This research study combined 2-hydroxyethyl cellulose (2HEC)—an etherified cellulose derivative with salicylic acid (SA) to develop a single layer and investigate the 2HEC viability as wound dressing material. Nine different samples with different compositions of SA, from 5 wt.% to 40 wt.% (with an interval of 5 wt.%) and one control sample without adding SA were prepared via the solution casting method. The 2HEC-SA films were studied regarding the effects of SA composition on antimicrobial properties ( Staphylococcus aureus) via the well-diffusion method. Additionally, degradability, mechanical properties, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) of 2HEC-SA films have also been tested. The strongest antimicrobial effect of 2HEC-SA film was obtained at 40 wt.% with a 16 mm inhibition zone diameter. There was a noticeable decreasing weight loss pattern in the degradation test and the tensile strength of 2HEC-SA film when the composition of salicylic acid is increased. 2HEC-SA film changes phases from amorphous to crystalline starting at 25 wt.% of salicylic acid as seen through XRD, while FTIR shows that complexation of 2HEC and salicylic acid occurred at 1050 cm<jats:sup>−1</jats:sup>.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141194328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-25DOI: 10.1177/08839115241256950
Adamu Biruk Fentahun, Gao Jing, Yuan Xiangnan, Tan Shaojie
The main aim of this study was to investigate the potential application of an indiginous Ethiopian medicinal plant, Croton macrostachyus, into PCL nanofibers through electrospinning for the first time. Croton macrostachyus (CM) leaf has antibacterial and wound healing properties. Croton macrostachyus of 1%, 3%, 5%, and 7% (w/v) incorporated PCL nanofibrous mats were produced through electrospinning. The produced nanofibrous mats morphology observed by scanning electron microscopy (SEM) was found to be continuous, bead-free, and interconnected. The average diameters of PCL, PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofibrous mat is 309 ± 114, 271 ± 66, 235 ± 56, 226 ± 65, and 216 ± 42 nm, respectively. According to the FTIR results, the CM leaf extract was successfully incorporated into the PCL nanofibers. The bacterial reduction percentage value of PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofiber mats against S. aureus is 77.00%, 99.88%, 99.91%, and 99.99%, and 35.00%, 78.00%, 90.00%, and 99.30% against E. coli. The in vitro release study showed that there was an immediate release of CM within 24 h and then a steady sustained release of 51.36, 53.92, 54.99, and 57.12% up to 72 h at 1%, 3%, 5%, and 7% concentration, respectively. The presence of CM leaf extract in the PCL nanofiber increased the hydrophilicity of the pure PCL nanofiber (100% hydrophilic at 7% leaf extract concentration). In general, the results of the in vitro study confirmed that the PCL nanofibrous mats loaded with CM leaf extract are suitable for use as an effective wound dressing with a broad spectrum of antimicrobial activity and hydrophilic properties.
{"title":"Indigenous Ethiopian traditional medicinal plant leaf extract, Croton macrostachyus, loaded PCL electrospun nanofibrous mat as potential wound dressing: In vitro analysis","authors":"Adamu Biruk Fentahun, Gao Jing, Yuan Xiangnan, Tan Shaojie","doi":"10.1177/08839115241256950","DOIUrl":"https://doi.org/10.1177/08839115241256950","url":null,"abstract":"The main aim of this study was to investigate the potential application of an indiginous Ethiopian medicinal plant, Croton macrostachyus, into PCL nanofibers through electrospinning for the first time. Croton macrostachyus (CM) leaf has antibacterial and wound healing properties. Croton macrostachyus of 1%, 3%, 5%, and 7% (w/v) incorporated PCL nanofibrous mats were produced through electrospinning. The produced nanofibrous mats morphology observed by scanning electron microscopy (SEM) was found to be continuous, bead-free, and interconnected. The average diameters of PCL, PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofibrous mat is 309 ± 114, 271 ± 66, 235 ± 56, 226 ± 65, and 216 ± 42 nm, respectively. According to the FTIR results, the CM leaf extract was successfully incorporated into the PCL nanofibers. The bacterial reduction percentage value of PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofiber mats against S. aureus is 77.00%, 99.88%, 99.91%, and 99.99%, and 35.00%, 78.00%, 90.00%, and 99.30% against E. coli. The in vitro release study showed that there was an immediate release of CM within 24 h and then a steady sustained release of 51.36, 53.92, 54.99, and 57.12% up to 72 h at 1%, 3%, 5%, and 7% concentration, respectively. The presence of CM leaf extract in the PCL nanofiber increased the hydrophilicity of the pure PCL nanofiber (100% hydrophilic at 7% leaf extract concentration). In general, the results of the in vitro study confirmed that the PCL nanofibrous mats loaded with CM leaf extract are suitable for use as an effective wound dressing with a broad spectrum of antimicrobial activity and hydrophilic properties.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141151493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Starch derived from plants plays an essential role in pharmaceuticals due to its components Amylose and Amylopectin, which form essential granules for drug delivery. Its biocompatibility and cost-effectiveness make it indispensable in pharmaceutical formulations, facilitating controlled drug release and tablet breakdown. The effectiveness and safety of drug formulations are often hindered by challenges such as low solubility and stability. In order to overcome these obstacles, current research is focused on modifying the properties of starch. The goal is to improve its solubility, swelling, erosion, stability, and ability to release drugs. A promising solution that has emerged is ultrasound-based modification. This technique has shown great potential in transforming starch granules, leading to improved solubility, degradability, and control over drug release. Not only is this method efficient and quick, but it also has the added benefit of being eco-friendly. This discussion will explore the mechanisms behind the modification of starch based on ultrasound, delving into both the physical and chemical changes that occur in starch granules. The analysis explores the utilization of modified starch induced by ultrasound in the field of drug delivery, investigating its stability and compatibility with biological systems. By exploring the capabilities and difficulties associated with the use of ultrasound to change starch for the delivery of drugs, we highlighted its potential as a leading and effective methodology.
{"title":"Ultrasound assisted techniques for starch modification to develop novel drug delivery systems: A comprehensive study","authors":"Vipin Kumar, Vinoth Kumarasamy, Pankaj Bhatt, Raghav Dixit, Mukesh Kumar, Chandra Prakash Shukla, Vetriselvan Subramaniyan, Sunil Kumar","doi":"10.1177/08839115241249143","DOIUrl":"https://doi.org/10.1177/08839115241249143","url":null,"abstract":"Starch derived from plants plays an essential role in pharmaceuticals due to its components Amylose and Amylopectin, which form essential granules for drug delivery. Its biocompatibility and cost-effectiveness make it indispensable in pharmaceutical formulations, facilitating controlled drug release and tablet breakdown. The effectiveness and safety of drug formulations are often hindered by challenges such as low solubility and stability. In order to overcome these obstacles, current research is focused on modifying the properties of starch. The goal is to improve its solubility, swelling, erosion, stability, and ability to release drugs. A promising solution that has emerged is ultrasound-based modification. This technique has shown great potential in transforming starch granules, leading to improved solubility, degradability, and control over drug release. Not only is this method efficient and quick, but it also has the added benefit of being eco-friendly. This discussion will explore the mechanisms behind the modification of starch based on ultrasound, delving into both the physical and chemical changes that occur in starch granules. The analysis explores the utilization of modified starch induced by ultrasound in the field of drug delivery, investigating its stability and compatibility with biological systems. By exploring the capabilities and difficulties associated with the use of ultrasound to change starch for the delivery of drugs, we highlighted its potential as a leading and effective methodology.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140965079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article presents an in-depth examination of recent advancements in medical and biotechnological sensing technologies, focusing on the forefront of innovation in hydrogel-based sensors within the domains of biomedical engineering and regenerative medicine. It delves into cutting-edge sensing technologies that facilitate non-invasive glucose monitoring, highlights progress in the development of intelligent solutions for wound care, and discusses the application of optical and fluorescence-based sensors for real-time diagnostics within the body. Further, it reviews the latest glucose monitoring devices, alongside wearable and implantable sensors designed for the continuous monitoring of health, including the measurement of physiological strain and stress. The exploration extends to the latest in non-invasive and minimally invasive technologies for ongoing health assessment, and to imaging and visualization techniques critical for medical diagnostics and therapeutic procedures. These advancements mark a pivotal move toward more efficient, precise, and patient-focused healthcare technologies, signaling new avenues for diagnosis, monitoring, and treatment in the healthcare sector.
{"title":"Hydrogels in biosensing and medical diagnostics","authors":"Hossein Omidian, Sumana Dey Chowdhury, Arnavaz Akhzarmehr","doi":"10.1177/08839115241253260","DOIUrl":"https://doi.org/10.1177/08839115241253260","url":null,"abstract":"This article presents an in-depth examination of recent advancements in medical and biotechnological sensing technologies, focusing on the forefront of innovation in hydrogel-based sensors within the domains of biomedical engineering and regenerative medicine. It delves into cutting-edge sensing technologies that facilitate non-invasive glucose monitoring, highlights progress in the development of intelligent solutions for wound care, and discusses the application of optical and fluorescence-based sensors for real-time diagnostics within the body. Further, it reviews the latest glucose monitoring devices, alongside wearable and implantable sensors designed for the continuous monitoring of health, including the measurement of physiological strain and stress. The exploration extends to the latest in non-invasive and minimally invasive technologies for ongoing health assessment, and to imaging and visualization techniques critical for medical diagnostics and therapeutic procedures. These advancements mark a pivotal move toward more efficient, precise, and patient-focused healthcare technologies, signaling new avenues for diagnosis, monitoring, and treatment in the healthcare sector.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140926199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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