Pub Date : 2024-03-18DOI: 10.1177/08839115241237327
Yue Xu, Xueyuan Liao, Zhengbi Liu, Lin Zhang
As a microscale three-dimensional bionic model, a microtissue array is a promising method for disease modeling and drug screening. Digital light processing (DLP) 3D bio-printing technology with the capabilities of excellent printing speed and resolution shows great potential for fabricating microtissue arrays. However, the outcomes of the microtissue arrays using DLP are limited by the shortage of biomaterials. In this work, we prepare hydrogel microtissues by combining DLP printing technology with biphasic bio-ink consisting of gelatin methacrylate (GelMA) and dextran. GelMA/ dextran is mixed to form bio-inks with different volume ratios, which can achieve high-precision printing of various patterns. Due to the presence of dextran, the bio-inks form the internal porous structure of the hydrogel in the printed patterns. The pore size is related to the proportion of dextran in bio-inks, and the pore size of hydrogel becomes smaller with the increase of dextran ratio. In addition, the adipose-derived stem cells (ADSCs) cells encapsulated in the porous hydrogels can maintain a high survival rate of 98.01% after 3 days of culture, and the larger pore size in the hydrogels promotes cell migration. Combining the DLP printing system with the porous hydrogel allows multiple micro-size geometric pattern hydrogels to be printed simultaneously to form microtissue arrays. In this study, a new bio-ink suitable for microtissue arrays is proposed, and a simple, accurate, and high-throughput biological manufacturing method for microtissue arrays is developed, thus providing a valuable tool for drug screening and tissue engineering.
{"title":"Digital light processing-based bioprinting of microtissue hydrogel arrays using dextran-induced aqueous emulsion ink","authors":"Yue Xu, Xueyuan Liao, Zhengbi Liu, Lin Zhang","doi":"10.1177/08839115241237327","DOIUrl":"https://doi.org/10.1177/08839115241237327","url":null,"abstract":"As a microscale three-dimensional bionic model, a microtissue array is a promising method for disease modeling and drug screening. Digital light processing (DLP) 3D bio-printing technology with the capabilities of excellent printing speed and resolution shows great potential for fabricating microtissue arrays. However, the outcomes of the microtissue arrays using DLP are limited by the shortage of biomaterials. In this work, we prepare hydrogel microtissues by combining DLP printing technology with biphasic bio-ink consisting of gelatin methacrylate (GelMA) and dextran. GelMA/ dextran is mixed to form bio-inks with different volume ratios, which can achieve high-precision printing of various patterns. Due to the presence of dextran, the bio-inks form the internal porous structure of the hydrogel in the printed patterns. The pore size is related to the proportion of dextran in bio-inks, and the pore size of hydrogel becomes smaller with the increase of dextran ratio. In addition, the adipose-derived stem cells (ADSCs) cells encapsulated in the porous hydrogels can maintain a high survival rate of 98.01% after 3 days of culture, and the larger pore size in the hydrogels promotes cell migration. Combining the DLP printing system with the porous hydrogel allows multiple micro-size geometric pattern hydrogels to be printed simultaneously to form microtissue arrays. In this study, a new bio-ink suitable for microtissue arrays is proposed, and a simple, accurate, and high-throughput biological manufacturing method for microtissue arrays is developed, thus providing a valuable tool for drug screening and tissue engineering.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"59 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140172306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to apply a novel green polymerization process to produce a new copolymer with potential antimicrobial activity for industrial application. The glycidol was polymerized into hyperbranched polyglycidol (HPG) polymer which in turn has been esterified with rosin to produce hyperbranched copoly(glycidol-Rosin) (HPGR), a new co-polymer. The process used Maghnite-H+, a montmorillonite silicate sheet clay, as an eco-catalyst and ultrasound was applied to enhance the interaction during polymerization. Different catalyst amounts were tested to assess their effects on the polymerization process. Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) were used to analyze the polymer products. The antimicrobial activity of HPGR was assessed against six human standard microbial strains using the agar disc diffusion method. The catalyst percentage of 10% resulted in the best yield for HPGR (35%). Thermogravimetric Analysis (TGA) revealed a best thermal stability of HPGR compared to that of HPG. The HPGR co-polymer displayed the best antibacterial activity against Klebsiella pneumonia, Bacillus cereus, Staphylococcus aureus, and Escherichia coli, producing inhibition zones of 12.33 ± 0.57, 11.00 ± 1.00, 10.66 ± 1.15, and 10.33 ± 1.15 mm, respectively. The hyperbranched copoly(glycidol-Rosin), an eco-catalyst-synthesized co-polymer, displayed interesting physical and antimicrobial properties for industrial application.
{"title":"Hyperbranched polyglycidol grafted with rosin by a natural catalyst under ultrasound: Synthesis, characterization and antimicrobial activity","authors":"Amina Mostefai, Mahmoud Belalia, Amine Harrane, Djahira Hamed, Mohammed Amin Bezzekhami, Louiza Belkacemi","doi":"10.1177/08839115241237258","DOIUrl":"https://doi.org/10.1177/08839115241237258","url":null,"abstract":"This study aimed to apply a novel green polymerization process to produce a new copolymer with potential antimicrobial activity for industrial application. The glycidol was polymerized into hyperbranched polyglycidol (HPG) polymer which in turn has been esterified with rosin to produce hyperbranched copoly(glycidol-Rosin) (HPGR), a new co-polymer. The process used Maghnite-H+, a montmorillonite silicate sheet clay, as an eco-catalyst and ultrasound was applied to enhance the interaction during polymerization. Different catalyst amounts were tested to assess their effects on the polymerization process. Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) were used to analyze the polymer products. The antimicrobial activity of HPGR was assessed against six human standard microbial strains using the agar disc diffusion method. The catalyst percentage of 10% resulted in the best yield for HPGR (35%). Thermogravimetric Analysis (TGA) revealed a best thermal stability of HPGR compared to that of HPG. The HPGR co-polymer displayed the best antibacterial activity against Klebsiella pneumonia, Bacillus cereus, Staphylococcus aureus, and Escherichia coli, producing inhibition zones of 12.33 ± 0.57, 11.00 ± 1.00, 10.66 ± 1.15, and 10.33 ± 1.15 mm, respectively. The hyperbranched copoly(glycidol-Rosin), an eco-catalyst-synthesized co-polymer, displayed interesting physical and antimicrobial properties for industrial application.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"87 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140105841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-05DOI: 10.1177/08839115241230969
Shungui Liu, Ziqiu Chen
Diabetes is a prevalent medical condition that often leads to complications, including diabetic wounds that can have serious consequences such as infections, amputations, and death. In the current research, lodoxamide was loaded into electrospun polycaprolacton/gelatin scaffolds In order to improve wound healing in a rat model. Our developed dressings were characterized in vitro using various methods and then applied on a rat model of excisional wound. Study showed that the dressings were not toxic and promoted migration potential of skin-derived fibroblast cells. Wound healing study showed that lodoxamide-loaded dressings had markedly higher healing potential than other scaffolds and augmented collagen deposition and epithelialization. Gene expression studies showed that TNF-α, glutathione peroxidase, and superoxide dismutase genes were significantly downregulated by lodoxamide-loaded scaffolds.
{"title":"Drug-eluting nanofibrous wound dressings composed of polycaprolactone and gelatin augment diabetic wounds healing via reducing oxidative stress and inflammation: An in vitro and in vivo study","authors":"Shungui Liu, Ziqiu Chen","doi":"10.1177/08839115241230969","DOIUrl":"https://doi.org/10.1177/08839115241230969","url":null,"abstract":"Diabetes is a prevalent medical condition that often leads to complications, including diabetic wounds that can have serious consequences such as infections, amputations, and death. In the current research, lodoxamide was loaded into electrospun polycaprolacton/gelatin scaffolds In order to improve wound healing in a rat model. Our developed dressings were characterized in vitro using various methods and then applied on a rat model of excisional wound. Study showed that the dressings were not toxic and promoted migration potential of skin-derived fibroblast cells. Wound healing study showed that lodoxamide-loaded dressings had markedly higher healing potential than other scaffolds and augmented collagen deposition and epithelialization. Gene expression studies showed that TNF-α, glutathione peroxidase, and superoxide dismutase genes were significantly downregulated by lodoxamide-loaded scaffolds.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"39 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140045672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-04DOI: 10.1177/08839115241237217
Hamid Omidian
This study chronicles the seminal contributions of Dr. Raphael M. Ottenbrite, the late Founding Editor of the Journal of Bioactive and Compatible Polymers, to polymer science from the 1970s through 2016. Beginning with an exploration of the biological impacts of anionic polymers, Dr. Ottenbrite’s research evolved to include their applications in immunology and cancer therapy. The 1980s marked a pivotal shift toward the synthesis of polymers geared specifically toward biomedical uses. In the 1990s, his work advanced to include sophisticated synthesis methods and surface modification techniques. The 2000s were characterized by a focus on nanotechnology and the innovation of drug delivery systems. His final research phase, from 2011 to 2016, concentrated on the development of biodegradable materials and advanced drug delivery systems. Dr. Ottenbrite’s work was a unique blend of polymer chemistry, materials science, and biomedicine, laying a vital groundwork for future interdisciplinary research.
{"title":"The enduring impact of a visionary scientist","authors":"Hamid Omidian","doi":"10.1177/08839115241237217","DOIUrl":"https://doi.org/10.1177/08839115241237217","url":null,"abstract":"This study chronicles the seminal contributions of Dr. Raphael M. Ottenbrite, the late Founding Editor of the Journal of Bioactive and Compatible Polymers, to polymer science from the 1970s through 2016. Beginning with an exploration of the biological impacts of anionic polymers, Dr. Ottenbrite’s research evolved to include their applications in immunology and cancer therapy. The 1980s marked a pivotal shift toward the synthesis of polymers geared specifically toward biomedical uses. In the 1990s, his work advanced to include sophisticated synthesis methods and surface modification techniques. The 2000s were characterized by a focus on nanotechnology and the innovation of drug delivery systems. His final research phase, from 2011 to 2016, concentrated on the development of biodegradable materials and advanced drug delivery systems. Dr. Ottenbrite’s work was a unique blend of polymer chemistry, materials science, and biomedicine, laying a vital groundwork for future interdisciplinary research.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"71 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140033415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biomaterials play an important role in biomedical applications serving as scaffolds in tissue engineering and substrates for cell growth by providing structural supports. Signaling cues for cellular events can be also presented, including cell viability, adhesion, spreading, and function developments. In this study, we fabricated a hierarchical nanofibrous scaffold through electrospinning silk fibroin (SF) and decellularized extracellular matrix of the small intestine submucosa (SIS-dECM). The addition of SIS-dECM improved the hydrophilic features of the resultant composite nanofibrous substrates as compared to SF nanofibrous substrates. The biocompatibility of the nanofibrous scaffolds was thoroughly investigated by culturing meningeal fibroblasts on SF and SF/SIS-dECM nanofibrous substrates. Cell viability, proliferation, focal adhesion, and quantitative measurements of cell adhesion-related gene expression levels revealed that the addition of SIS-dECM favored the adhesion and spreading of fibroblast cells. Therefore, the SF/SIS-dECM nanofibers provided a suitable substrate for cell growth and the present study suggested that the nanofibrous substrates hold high potential for bioapplications.
生物材料在生物医学应用中发挥着重要作用，它既是组织工程中的支架，也是通过提供结构支撑促进细胞生长的基质。生物材料还能为细胞事件提供信号线索，包括细胞活力、粘附、扩散和功能发展。在这项研究中，我们通过电纺丝（SF）和脱细胞小肠粘膜下细胞外基质（SIS-dECM）制造了一种分层纳米纤维支架。与 SF 纳米纤维基底相比，SIS-dECM 的加入改善了所得到的复合纳米纤维基底的亲水性。通过在 SF 和 SF/SIS-dECM 纳米纤维基底上培养脑膜成纤维细胞，对纳米纤维支架的生物相容性进行了深入研究。细胞活力、增殖、灶粘附和细胞粘附相关基因表达水平的定量测量结果表明，添加 SIS-dECM 有利于成纤维细胞的粘附和扩散。因此，SF/SIS-dECM 纳米纤维为细胞生长提供了合适的基底，本研究表明纳米纤维基底在生物应用方面具有很大的潜力。
{"title":"Electrospun silk fibroin/SIS-dECM hierarchical scaffolds for cell growth","authors":"Lusi Chen, Yahao Ma, Longyou Xiao, Pengfei Xie, Jianghui Liu, Wanmei Li, Xiaoying Wang, Liumin He","doi":"10.1177/08839115241233344","DOIUrl":"https://doi.org/10.1177/08839115241233344","url":null,"abstract":"Biomaterials play an important role in biomedical applications serving as scaffolds in tissue engineering and substrates for cell growth by providing structural supports. Signaling cues for cellular events can be also presented, including cell viability, adhesion, spreading, and function developments. In this study, we fabricated a hierarchical nanofibrous scaffold through electrospinning silk fibroin (SF) and decellularized extracellular matrix of the small intestine submucosa (SIS-dECM). The addition of SIS-dECM improved the hydrophilic features of the resultant composite nanofibrous substrates as compared to SF nanofibrous substrates. The biocompatibility of the nanofibrous scaffolds was thoroughly investigated by culturing meningeal fibroblasts on SF and SF/SIS-dECM nanofibrous substrates. Cell viability, proliferation, focal adhesion, and quantitative measurements of cell adhesion-related gene expression levels revealed that the addition of SIS-dECM favored the adhesion and spreading of fibroblast cells. Therefore, the SF/SIS-dECM nanofibers provided a suitable substrate for cell growth and the present study suggested that the nanofibrous substrates hold high potential for bioapplications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"6 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139952131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-16DOI: 10.1177/08839115241230970
Kanchan Parate, Pratibha Pandey
Chitosan nanoparticles (CSNP) with an average size of 25.67 nm were synthesized via a novel wet chemical route and characterized using scanning electron microscopy (SEM) and Fourier Transform Infrared (FTIR) spectroscopy. Comparative antibacterial assays of Chitosan NP suspensions prepared in water (at neutral pH) and in dilute acetic acid and chitosan gel prepared in dilute acetic acid/hydrochloric acid (all at concentrations up to 1%) were performed against Escherichia coli ( E. coli) and Bacillus subtilis ( B. subtilis) bacteria using spread plate method. A parallel viability test was conducted to confirm the presence of surviving cells in the bulk test volume. Minimum bactericidal concentration (MBC) of chitosan gel was 0.5 mg/ml for the bulk chitosan dissolved in 0.05% v/v aq. acetic acid and 0.1 mg/ml for chitosan dissolved in 0.01% v/v aqueous (aq.) hydrochloric acid. In comparison, Chitosan NP were found to be growth promoter at neutral pH and exhibited cell protective efficacy in presence of aq. acetic acid. The biocidal activity of chitosan gel in acidic media was higher when prepared in strong inorganic acid, that is, aq. HCl in comparison with the gel prepared in a relatively weak organic acid that is, aq. CH3COOH at the same concentration. Antibacterial action also showed pH dependence with higher activity at lower pH. However, respective aq. acids also gave comparable bactericidal action; indicating that chitosan may not have any inherent antibacterial property and basically it acts as a growth promoter.
{"title":"Bioactivity of chitosan nanoparticles synthesized by a novel route towards Escherichia coli and Bacillus subtilis","authors":"Kanchan Parate, Pratibha Pandey","doi":"10.1177/08839115241230970","DOIUrl":"https://doi.org/10.1177/08839115241230970","url":null,"abstract":"Chitosan nanoparticles (CSNP) with an average size of 25.67 nm were synthesized via a novel wet chemical route and characterized using scanning electron microscopy (SEM) and Fourier Transform Infrared (FTIR) spectroscopy. Comparative antibacterial assays of Chitosan NP suspensions prepared in water (at neutral pH) and in dilute acetic acid and chitosan gel prepared in dilute acetic acid/hydrochloric acid (all at concentrations up to 1%) were performed against Escherichia coli ( E. coli) and Bacillus subtilis ( B. subtilis) bacteria using spread plate method. A parallel viability test was conducted to confirm the presence of surviving cells in the bulk test volume. Minimum bactericidal concentration (MBC) of chitosan gel was 0.5 mg/ml for the bulk chitosan dissolved in 0.05% v/v aq. acetic acid and 0.1 mg/ml for chitosan dissolved in 0.01% v/v aqueous (aq.) hydrochloric acid. In comparison, Chitosan NP were found to be growth promoter at neutral pH and exhibited cell protective efficacy in presence of aq. acetic acid. The biocidal activity of chitosan gel in acidic media was higher when prepared in strong inorganic acid, that is, aq. HCl in comparison with the gel prepared in a relatively weak organic acid that is, aq. CH<jats:sub>3</jats:sub>COOH at the same concentration. Antibacterial action also showed pH dependence with higher activity at lower pH. However, respective aq. acids also gave comparable bactericidal action; indicating that chitosan may not have any inherent antibacterial property and basically it acts as a growth promoter.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"43 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139956390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-16DOI: 10.1177/08839115241233345
Luan dos Santos Menezes, Daniel Navarro da Rocha, Renato Carajelescov Nonato, Ana Rosa Costa, Ana Rita Morales, Lourenço Correr-Sobrinho, Américo Bortolazzo Correr, José Guilherme Neves
The aim of this study was to synthesize and characterize Cellulose Acetate (CA) porous scaffolds using the electrospinning technique associated with Hydroxyapatite (HA) and different concentrations of graphene oxide (GO), for advanced regenerative therapies application. The scaffolds were categorized into four distinct groups based on their composition: (1) Pure CA scaffolds; (2) CAHA scaffolds; (3) CAHAGO 1.0% scaffolds; (4) CAHAGO 1.5% scaffolds. Transmission Electron Microscopy (TEM) was used for the characterization of the nanocomposite. The scaffolds were analyzed by X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, Scanning Electron Microscopy with Energy Dispersive Spectroscopy (SEM/EDS), and in vitro cell viability assays (WST). For the biological test analysis of Variance (two-way) was used, followed by Tukey’s post-test (α = 0.05). The TEM analysis allowed for the visualization of the deposition of HA on the graphene sheets, confirming the synthesis of the nanocomposite. XRD revealed the predominant presence of CaP phases in the CAHA, CAHAGO 1.0%, and CAHAGO 1.5% groups, underscoring the inherent mineral composition of the scaffolds. FTIR demonstrated cellulose characteristics and PO4 bands in the groups containing HA, confirming the effective incorporation of this material. Raman spectroscopy revealed distinct peaks in the GO groups, conclusively verifying the successful integration of graphene into the scaffold matrix. The micrographs showcased irregular pores filling the entire surface, arising from the intricate overlapping of fibers during scaffold formation. Importantly, all scaffolds exhibited excellent cell viability in the conducted assays. A proliferation process was observed in CAHA and CAHAGO 1.5% groups after 48 h ( p < 0.05). In conclusion, the scaffolds synthesized hold significant promise in the realm of tissue engineering and provide a fresh perspective on the possibilities for regenerative therapies.
本研究旨在利用电纺丝技术合成醋酸纤维素（CA）多孔支架并对其进行表征，同时将其与羟基磷灰石（HA）和不同浓度的氧化石墨烯（GO）结合起来，用于先进的再生疗法。这些支架根据其成分分为四组：(1) 纯 CA 支架；(2) CAHA 支架；(3) CAHAGO 1.0% 支架；(4) CAHAGO 1.5% 支架。纳米复合材料的表征采用了透射电子显微镜（TEM）。通过 X 射线衍射 (XRD)、傅立叶变换红外光谱 (FTIR)、拉曼光谱、扫描电子显微镜与能量色散光谱 (SEM/EDS) 和体外细胞活力检测 (WST) 对支架进行了分析。生物测试采用方差分析（双向），然后进行 Tukey 后检验（α = 0.05）。通过 TEM 分析可观察到 HA 在石墨烯片上的沉积，从而证实了纳米复合材料的合成。XRD 显示，在 CAHA、CAHAGO 1.0% 和 CAHAGO 1.5% 组中主要存在 CaP 相，突出了支架固有的矿物成分。傅立叶变换红外光谱（FTIR）显示了含有 HA 的组中的纤维素特征和 PO4 带，证实了这种材料的有效加入。拉曼光谱在 GO 组中显示出明显的峰值，最终验证了石墨烯与支架基质的成功结合。显微照片显示，整个表面充满了不规则的孔隙，这是支架形成过程中纤维错综复杂的重叠造成的。重要的是，所有支架在实验中都表现出极佳的细胞活力。48 小时后，在 CAHA 和 CAHAGO 1.5% 组中观察到了细胞增殖过程（p < 0.05）。总之，合成的支架在组织工程领域大有可为，为再生疗法提供了一个全新的视角。
{"title":"Cellulose acetate scaffold containing hydroxyapatite/graphene oxide nanocomposite by electrospinning for advanced regenerative therapies","authors":"Luan dos Santos Menezes, Daniel Navarro da Rocha, Renato Carajelescov Nonato, Ana Rosa Costa, Ana Rita Morales, Lourenço Correr-Sobrinho, Américo Bortolazzo Correr, José Guilherme Neves","doi":"10.1177/08839115241233345","DOIUrl":"https://doi.org/10.1177/08839115241233345","url":null,"abstract":"The aim of this study was to synthesize and characterize Cellulose Acetate (CA) porous scaffolds using the electrospinning technique associated with Hydroxyapatite (HA) and different concentrations of graphene oxide (GO), for advanced regenerative therapies application. The scaffolds were categorized into four distinct groups based on their composition: (1) Pure CA scaffolds; (2) CAHA scaffolds; (3) CAHAGO 1.0% scaffolds; (4) CAHAGO 1.5% scaffolds. Transmission Electron Microscopy (TEM) was used for the characterization of the nanocomposite. The scaffolds were analyzed by X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, Scanning Electron Microscopy with Energy Dispersive Spectroscopy (SEM/EDS), and in vitro cell viability assays (WST). For the biological test analysis of Variance (two-way) was used, followed by Tukey’s post-test (α = 0.05). The TEM analysis allowed for the visualization of the deposition of HA on the graphene sheets, confirming the synthesis of the nanocomposite. XRD revealed the predominant presence of CaP phases in the CAHA, CAHAGO 1.0%, and CAHAGO 1.5% groups, underscoring the inherent mineral composition of the scaffolds. FTIR demonstrated cellulose characteristics and PO<jats:sub>4</jats:sub> bands in the groups containing HA, confirming the effective incorporation of this material. Raman spectroscopy revealed distinct peaks in the GO groups, conclusively verifying the successful integration of graphene into the scaffold matrix. The micrographs showcased irregular pores filling the entire surface, arising from the intricate overlapping of fibers during scaffold formation. Importantly, all scaffolds exhibited excellent cell viability in the conducted assays. A proliferation process was observed in CAHA and CAHAGO 1.5% groups after 48 h ( p < 0.05). In conclusion, the scaffolds synthesized hold significant promise in the realm of tissue engineering and provide a fresh perspective on the possibilities for regenerative therapies.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"4 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139952126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-04DOI: 10.1177/08839115231223963
Maryam Safari, S. Fakhri, Hassan Maleki, Mohammad Hosein Farzaei
Peripheral neuropathies are associated with various detrimental complications, leading to life-debilitating disorders, including neuropathic pain. Hence, the current study aimed to incorporate naringin, a potential natural component, into chitosan nanoparticles (NPs) to ameliorate the complications resulting from chronic constriction injury (CCI) induced painful neuropathy. The prepared NPs had a particle size of 220 nm and PDI = 0.37, with relatively spherical morphology and zeta potential of +41.5 mV. The relevant analyses indicated the loading and high encapsulation efficiency of naringin into the NPs as well as a prolonged release of naringin. The anti-neuropathic evaluations of chronic constriction injury (CCI)-induced rats treated with naringin-loaded NPs (10 mg/kg) remarkably ameliorated hyperalgesia and cold allodynia. In addition, the treatment with naringin-loaded NPs led to improvements in sensory and locomotor impairment, as evidenced by changes in behavioral parameters such as reduced paw licking, increased rearings, and enhanced crossings. The NPs treatment significantly attenuated the elevated levels of nitrite and restored the reduced glutathione level in the serum of CCI-induced rats. Moreover, histopathological analysis exhibited regeneration of the sciatic nerve injury through reducing myelin degeneration, axonal swelling, and nerve fiber derangement. Therefore, these findings suggest that the naringin-loaded chitosan NPs has promising pharmacological activities for the treatment of neuropathic pain sufferers.
{"title":"Evaluating the anti-neuropathic effects of naringin-loaded chitosan nanocarriers in a murine model of constriction injury","authors":"Maryam Safari, S. Fakhri, Hassan Maleki, Mohammad Hosein Farzaei","doi":"10.1177/08839115231223963","DOIUrl":"https://doi.org/10.1177/08839115231223963","url":null,"abstract":"Peripheral neuropathies are associated with various detrimental complications, leading to life-debilitating disorders, including neuropathic pain. Hence, the current study aimed to incorporate naringin, a potential natural component, into chitosan nanoparticles (NPs) to ameliorate the complications resulting from chronic constriction injury (CCI) induced painful neuropathy. The prepared NPs had a particle size of 220 nm and PDI = 0.37, with relatively spherical morphology and zeta potential of +41.5 mV. The relevant analyses indicated the loading and high encapsulation efficiency of naringin into the NPs as well as a prolonged release of naringin. The anti-neuropathic evaluations of chronic constriction injury (CCI)-induced rats treated with naringin-loaded NPs (10 mg/kg) remarkably ameliorated hyperalgesia and cold allodynia. In addition, the treatment with naringin-loaded NPs led to improvements in sensory and locomotor impairment, as evidenced by changes in behavioral parameters such as reduced paw licking, increased rearings, and enhanced crossings. The NPs treatment significantly attenuated the elevated levels of nitrite and restored the reduced glutathione level in the serum of CCI-induced rats. Moreover, histopathological analysis exhibited regeneration of the sciatic nerve injury through reducing myelin degeneration, axonal swelling, and nerve fiber derangement. Therefore, these findings suggest that the naringin-loaded chitosan NPs has promising pharmacological activities for the treatment of neuropathic pain sufferers.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"56 25","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139384742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.1177/08839115231218732
Oruç Numan Gökçe, D. Aykora, Merve Danışman, Ufuk Demir, Cemre Aydeğer, S. C. Suner, Ayhan Oral, İhsan Karaboğa, Metehan Uzun
Abdominal adhesions are still among the most common postsurgical peritoneal inflammation-related complications. Adhesion-related disorders are still highly costly and prevalent due to advances in surgical techniques, treatment methods, and various drugs. The present study aimed to investigate the effects of Poly ( D,L-lactide-co-glycolide) (PLGA)- polyethylene glycol (PEG6000) Nanofiber + Melatonin on the abdominal adhesion model in rats. For this purpose, PLGA-PEG6000 Nanofiber + Melatonin matrix was fabricated and implanted in an experimental abdominal adhesion model in rats. Our study consisted of an in vitro and an in vivo part. The degradation and release profile of the matrix and Melatonin (Mel) embedded matrix was performed in vitro. In vivo, the procedure was carried out with 18 Wistar male rats. Rats were divided into three groups as follows: Sham, Matrix, and Mel + Matrix, respectively. Consequent to degradation and release profiling in vitro, an experimental adhesion model was created and fabricated pure matrix (2 × 2 cm2), and matrix (2 × 2 cm2, 0.25 mg melatonin/per matrix embedded) was applied to injury area in related groups. Intra-abdominal adhesion scores were determined on post-op 21st day, under general anesthesia. Following, cecum, peritoneal tissue, and adhesive bands were harvested. Macroscopic analysis (severity of adhesion formation), Hematoxlyn&Eosin and Masson’s Trichrome staining (for the examination of the levels of infiltration of inflammatory cells, fibrosis, and neovascularization) were performed for the evaluation of the effects of Mel embedded and pure matrix Our results indicated that PLGA-PEG6000 Nanofiber + Melatonin matrix was degraded completely in rats abdominal cavity and significantly reduced adhesion formation compared to other groups macroscopically ( p < 0.05). On the other hand, the histopathological analysis indicated that the fabricated matrix reduced inflammatory cell infiltration, fibrosis, and neovascularization levels.
{"title":"Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation","authors":"Oruç Numan Gökçe, D. Aykora, Merve Danışman, Ufuk Demir, Cemre Aydeğer, S. C. Suner, Ayhan Oral, İhsan Karaboğa, Metehan Uzun","doi":"10.1177/08839115231218732","DOIUrl":"https://doi.org/10.1177/08839115231218732","url":null,"abstract":"Abdominal adhesions are still among the most common postsurgical peritoneal inflammation-related complications. Adhesion-related disorders are still highly costly and prevalent due to advances in surgical techniques, treatment methods, and various drugs. The present study aimed to investigate the effects of Poly ( D,L-lactide-co-glycolide) (PLGA)- polyethylene glycol (PEG6000) Nanofiber + Melatonin on the abdominal adhesion model in rats. For this purpose, PLGA-PEG6000 Nanofiber + Melatonin matrix was fabricated and implanted in an experimental abdominal adhesion model in rats. Our study consisted of an in vitro and an in vivo part. The degradation and release profile of the matrix and Melatonin (Mel) embedded matrix was performed in vitro. In vivo, the procedure was carried out with 18 Wistar male rats. Rats were divided into three groups as follows: Sham, Matrix, and Mel + Matrix, respectively. Consequent to degradation and release profiling in vitro, an experimental adhesion model was created and fabricated pure matrix (2 × 2 cm2), and matrix (2 × 2 cm2, 0.25 mg melatonin/per matrix embedded) was applied to injury area in related groups. Intra-abdominal adhesion scores were determined on post-op 21st day, under general anesthesia. Following, cecum, peritoneal tissue, and adhesive bands were harvested. Macroscopic analysis (severity of adhesion formation), Hematoxlyn&Eosin and Masson’s Trichrome staining (for the examination of the levels of infiltration of inflammatory cells, fibrosis, and neovascularization) were performed for the evaluation of the effects of Mel embedded and pure matrix Our results indicated that PLGA-PEG6000 Nanofiber + Melatonin matrix was degraded completely in rats abdominal cavity and significantly reduced adhesion formation compared to other groups macroscopically ( p < 0.05). On the other hand, the histopathological analysis indicated that the fabricated matrix reduced inflammatory cell infiltration, fibrosis, and neovascularization levels.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"16 12","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138589797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.1177/08839115231216999
Yan Li, Qunzi Ge, Lie Ma
Mesenchymal stem cells (MSCs) possess self-renewal ability, multi-differentiation potential and low immunogenicity, thus serving as an ideal choice for cell therapies. Ex-vivo expansion systems that have been developed to meet clinical demands are faced with two crucial barriers, limited quantity and stemness loss of expanded cells. Hence, it is crucial and feasible to construct microcarriers that can show high and specific affinity to MSCs, and support highly efficient cell expansion with minimal stemness loss. In this study, EPLQLKM (E7) peptides were modified onto gelatin microcarriers by poly (ethylene glycol) (PEG) linkers, which showed great antifouling ability against xenogenic components. The rat bone marrow-derived mesenchymal stem cells (rBMSCs) harvested from the E7-modified gelatin microcarriers achieved better cell attachment, stemness maintenance, viability, and multilineage differentiation potentials, especially those with a higher E7 density. Attributing to the promotion for cell adhesion, E7 functionalization increased the expansion efficiency of rBMSCs with improved quantity and quality simultaneously, thereby providing a novel strategy for scalable expansion to optimize the clinical performance of MSCs.
{"title":"E7-modified gelatin microcarriers for efficient expansion and stemness preservation of mesenchymal stem cells","authors":"Yan Li, Qunzi Ge, Lie Ma","doi":"10.1177/08839115231216999","DOIUrl":"https://doi.org/10.1177/08839115231216999","url":null,"abstract":"Mesenchymal stem cells (MSCs) possess self-renewal ability, multi-differentiation potential and low immunogenicity, thus serving as an ideal choice for cell therapies. Ex-vivo expansion systems that have been developed to meet clinical demands are faced with two crucial barriers, limited quantity and stemness loss of expanded cells. Hence, it is crucial and feasible to construct microcarriers that can show high and specific affinity to MSCs, and support highly efficient cell expansion with minimal stemness loss. In this study, EPLQLKM (E7) peptides were modified onto gelatin microcarriers by poly (ethylene glycol) (PEG) linkers, which showed great antifouling ability against xenogenic components. The rat bone marrow-derived mesenchymal stem cells (rBMSCs) harvested from the E7-modified gelatin microcarriers achieved better cell attachment, stemness maintenance, viability, and multilineage differentiation potentials, especially those with a higher E7 density. Attributing to the promotion for cell adhesion, E7 functionalization increased the expansion efficiency of rBMSCs with improved quantity and quality simultaneously, thereby providing a novel strategy for scalable expansion to optimize the clinical performance of MSCs.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"76 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138587044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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