Pub Date : 2023-02-22DOI: 10.1177/08839115221149725
Azam Bozorgi, M. Khazaei, Maryam Bozorgi, Z. Jamalpoor
Fabricating degradable polymer-based membranes has attracted much attention for guided bone regeneration. Chitosan/gelatin (Cs/Gel) composites are among the most known scaffolds with structural similarity to bone matrix and a high potential to support cell attachment and proliferation. Recently, plant-derived phenolic compound apigenin has been identified to direct the osteogenic differentiation of mesenchymal stem cells and retain osteoblast metabolic functions. We incorporated apigenin into Cs/Gel membranes to improve apigenin bioavailability and get proper concentrations for efficient biological activities. Apigenin-loaded Cs/Gel membranes were prepared using a solution casting method with various apigenin contents (0, 10, 25, 50, and 100 µM). Chemical composition, morphological characteristics, swelling behavior, degradation rate, and apigenin release from membranes were evaluated. Saos-2 osteoblasts were cultured on membranes to investigate cell-membrane interaction, proliferation, viability, and mineralization under the osteogenic culture condition. The results showed that membranes had homogeneous and moderate rough surfaces, facilitating osteoblast attachment and expansion. Swelling ratios exceeded 200%, reaching a stable rate in 24 h. Apigenin-loaded membranes degraded slower in vitro. Membranes containing lower apigenin concentrations exhibited a higher cargo release profile over 21 days. Apigenin improved osteoblast proliferation and viability, but the mineralization depended on apigenin dose, with optimized values at low concentrations. These data suggested that Cs/Gel membranes loaded with low apigenin contents improved osteoblast survival, proliferation, and mineralization.
{"title":"Fabrication and characterization of apigenin-loaded chitosan/gelatin membranes for bone tissue engineering applications","authors":"Azam Bozorgi, M. Khazaei, Maryam Bozorgi, Z. Jamalpoor","doi":"10.1177/08839115221149725","DOIUrl":"https://doi.org/10.1177/08839115221149725","url":null,"abstract":"Fabricating degradable polymer-based membranes has attracted much attention for guided bone regeneration. Chitosan/gelatin (Cs/Gel) composites are among the most known scaffolds with structural similarity to bone matrix and a high potential to support cell attachment and proliferation. Recently, plant-derived phenolic compound apigenin has been identified to direct the osteogenic differentiation of mesenchymal stem cells and retain osteoblast metabolic functions. We incorporated apigenin into Cs/Gel membranes to improve apigenin bioavailability and get proper concentrations for efficient biological activities. Apigenin-loaded Cs/Gel membranes were prepared using a solution casting method with various apigenin contents (0, 10, 25, 50, and 100 µM). Chemical composition, morphological characteristics, swelling behavior, degradation rate, and apigenin release from membranes were evaluated. Saos-2 osteoblasts were cultured on membranes to investigate cell-membrane interaction, proliferation, viability, and mineralization under the osteogenic culture condition. The results showed that membranes had homogeneous and moderate rough surfaces, facilitating osteoblast attachment and expansion. Swelling ratios exceeded 200%, reaching a stable rate in 24 h. Apigenin-loaded membranes degraded slower in vitro. Membranes containing lower apigenin concentrations exhibited a higher cargo release profile over 21 days. Apigenin improved osteoblast proliferation and viability, but the mineralization depended on apigenin dose, with optimized values at low concentrations. These data suggested that Cs/Gel membranes loaded with low apigenin contents improved osteoblast survival, proliferation, and mineralization.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91028561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-07DOI: 10.1177/08839115221149723
Patrick N. Charron, Irfan Tahir, Sierra N McConnell, Danielle Sedler, R. Floreani
A leading cancer diagnosis in women worldwide is cervical cancer, with current treatments all posing a risk of serious side effects. Less toxic, but effective treatments are sought after. Aloe vera (barbadensis miller), known for its beneficial properties, has been studied for cancer treatment. While aloe gel has been shown to exhibit anti-cancer activity, it cannot form a hydrogel alone. Therefore, an interpenetrating network comprising alginate blended with aloe was examined as a cervical cancer treatment. We hypothesized the antioxidant properties of aloe gel would decrease cancer cell viability while the alginate hydrogel would improve mucoadhesion. We further hypothesized the antioxidant activity of aloe gel would induce cancer cell death at levels similar to common chemotherapeutics, and aimed to determine if these chemotherapeutic behaviors are constructive or destructive. Material and adhesive properties, drug encapsulation, and cancer cell viability were investigated and validated. The effect of aloe-alginate hydrogels on cervical cancer cell viability was not significantly different compared to aloe-blends containing doxorubicin (DOX), indicating that the aloe alone decreased cancer cell viability rendering the additional cytotoxic therapeutic not impactful as an adjuvant therapy. This study provides insight into the potential of natural biopolymers for treating cervical cancer without systemic toxic compounds.
{"title":"Physico-mechanical and ex vivo analysis of aloe-alginate hydrogels for cervical cancer treatment","authors":"Patrick N. Charron, Irfan Tahir, Sierra N McConnell, Danielle Sedler, R. Floreani","doi":"10.1177/08839115221149723","DOIUrl":"https://doi.org/10.1177/08839115221149723","url":null,"abstract":"A leading cancer diagnosis in women worldwide is cervical cancer, with current treatments all posing a risk of serious side effects. Less toxic, but effective treatments are sought after. Aloe vera (barbadensis miller), known for its beneficial properties, has been studied for cancer treatment. While aloe gel has been shown to exhibit anti-cancer activity, it cannot form a hydrogel alone. Therefore, an interpenetrating network comprising alginate blended with aloe was examined as a cervical cancer treatment. We hypothesized the antioxidant properties of aloe gel would decrease cancer cell viability while the alginate hydrogel would improve mucoadhesion. We further hypothesized the antioxidant activity of aloe gel would induce cancer cell death at levels similar to common chemotherapeutics, and aimed to determine if these chemotherapeutic behaviors are constructive or destructive. Material and adhesive properties, drug encapsulation, and cancer cell viability were investigated and validated. The effect of aloe-alginate hydrogels on cervical cancer cell viability was not significantly different compared to aloe-blends containing doxorubicin (DOX), indicating that the aloe alone decreased cancer cell viability rendering the additional cytotoxic therapeutic not impactful as an adjuvant therapy. This study provides insight into the potential of natural biopolymers for treating cervical cancer without systemic toxic compounds.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87326096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-07DOI: 10.1177/08839115221143445
Meiling Shao, Zhan Shi, Chi Zhang, Zhongyi Li, B. Zhai
As a biosynthetic polymer, Bacterial cellulose (BC) has been largely used in biomedical and technological fields for the excellent biocompatibility and water holding capability. In this study, BC hydrogel were mass-produced from G. xylinus. A novel gel, BC nanocomposite (BC/NC) hydrogel, was prepared via in situ free radical aqueous polymerization from NIPAM in the presence of Clay was added as physical crosslinker. The physical and chemical properties were evaluated, and the results showed that the properties of the composite hydrogel were improved, for example, the Young’s modulus rose by nearly 30%, from 4.7 to 6.0 Mpa with the increasing of NIPAM. BC/NC-lys hydrogel were prepared by treating BC/NC hydrogel with Lysostaphin solution, and the cytocompatibility and antibacterial activities were assessed in vitro. The effects of composite hydrogel on wound healing were examined in rat skin models, the cure rate was up to 92.35% in the test group and only 78.83% in the control group after 14 days. The composite BC/NC3-lys hydrogel were developed in the hope of accelerating the wound healing process as well as decreasing the infection rate.
{"title":"Preparation and performance of bacterial cellulose-based enzyme-carrying composite hydrogels as wound healing material","authors":"Meiling Shao, Zhan Shi, Chi Zhang, Zhongyi Li, B. Zhai","doi":"10.1177/08839115221143445","DOIUrl":"https://doi.org/10.1177/08839115221143445","url":null,"abstract":"As a biosynthetic polymer, Bacterial cellulose (BC) has been largely used in biomedical and technological fields for the excellent biocompatibility and water holding capability. In this study, BC hydrogel were mass-produced from G. xylinus. A novel gel, BC nanocomposite (BC/NC) hydrogel, was prepared via in situ free radical aqueous polymerization from NIPAM in the presence of Clay was added as physical crosslinker. The physical and chemical properties were evaluated, and the results showed that the properties of the composite hydrogel were improved, for example, the Young’s modulus rose by nearly 30%, from 4.7 to 6.0 Mpa with the increasing of NIPAM. BC/NC-lys hydrogel were prepared by treating BC/NC hydrogel with Lysostaphin solution, and the cytocompatibility and antibacterial activities were assessed in vitro. The effects of composite hydrogel on wound healing were examined in rat skin models, the cure rate was up to 92.35% in the test group and only 78.83% in the control group after 14 days. The composite BC/NC3-lys hydrogel were developed in the hope of accelerating the wound healing process as well as decreasing the infection rate.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75253410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-21DOI: 10.1177/08839115221149724
M. Attia, M. A. Khalf, Mahmoud Abou‐Okada, M. Shamseldean, M. Salem, M. N. Al-Sabi
This study evaluates the use of chitosan–silver nanocomposites (CSN) as an insecticide against the bed bug (Cimex lectularius). Adult bed bugs were collected from infested residential areas and identified using light microscopy and scanning electron microscopy. CSN were prepared and photographed for characterization using transmission electron microscopy, dynamic light scattering, and zeta potential. The insecticidal effect of different concentrations of CSN (400–1000 ppm) was compared to that of 0.1% cypermethrin as a positive control and normal saline as a negative control. The bugs (n = 25) were immersed for 20 min in the corresponding medium, dried with filter papers, and then incubated at 27–28°C and 70% RH with a 12:12 h light–dark photoperiod. The mortality rates were recorded at different time intervals (2, 4, 6, 12, and 24 h post-incubation (hPI)), and the entire experiment was repeated five times. Image analysis showed round- to spherical-shaped CSN ranging in size from 34 to 72 nm. The mortality rates were positively associated with increasing concentrations of CSN. The mortality rate first reached 100% for concentrations of 800 ppm at 24 hPI and 1000 ppm at 12 hPI. The calculated LC50 was found at a concentration of 1165 ppm at 2 hPI, and the LC99 was found at a concentration of 1914 ppm at 2 hPI. The positive control, cypermethrin, induced 100% mortality among the bugs at 2 hPI, while the negative control caused no mortality. These results clearly show the potential of CSN as an insecticide against C. lectularius. Future studies on best practices for implementing these particles in clinical settings are recommended.
{"title":"Chitosan–silver nanocomposites as a promising tool for controlling the bed bug: Cimex lectularius (Heteroptera: Cimicidae)","authors":"M. Attia, M. A. Khalf, Mahmoud Abou‐Okada, M. Shamseldean, M. Salem, M. N. Al-Sabi","doi":"10.1177/08839115221149724","DOIUrl":"https://doi.org/10.1177/08839115221149724","url":null,"abstract":"This study evaluates the use of chitosan–silver nanocomposites (CSN) as an insecticide against the bed bug (Cimex lectularius). Adult bed bugs were collected from infested residential areas and identified using light microscopy and scanning electron microscopy. CSN were prepared and photographed for characterization using transmission electron microscopy, dynamic light scattering, and zeta potential. The insecticidal effect of different concentrations of CSN (400–1000 ppm) was compared to that of 0.1% cypermethrin as a positive control and normal saline as a negative control. The bugs (n = 25) were immersed for 20 min in the corresponding medium, dried with filter papers, and then incubated at 27–28°C and 70% RH with a 12:12 h light–dark photoperiod. The mortality rates were recorded at different time intervals (2, 4, 6, 12, and 24 h post-incubation (hPI)), and the entire experiment was repeated five times. Image analysis showed round- to spherical-shaped CSN ranging in size from 34 to 72 nm. The mortality rates were positively associated with increasing concentrations of CSN. The mortality rate first reached 100% for concentrations of 800 ppm at 24 hPI and 1000 ppm at 12 hPI. The calculated LC50 was found at a concentration of 1165 ppm at 2 hPI, and the LC99 was found at a concentration of 1914 ppm at 2 hPI. The positive control, cypermethrin, induced 100% mortality among the bugs at 2 hPI, while the negative control caused no mortality. These results clearly show the potential of CSN as an insecticide against C. lectularius. Future studies on best practices for implementing these particles in clinical settings are recommended.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84049294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.1177/08839115221143443
X. Le, T. Tong, Thanh Tung Le, Pham C. T. Dung, L. Bach
A method for purifying the polysaccharides from Sterculia foetida L. gum was developed. The effects of purifying conditions were also studied. Results have shown that the conditions of pH 0.5, temperature of 80°C, and 2.5-h reaction duration gave the best polysaccharides yield. The mineral content and the molecular weight of the obtained polysaccharides were measured for characterization. The cross-linking of the polysaccharides with citric acid in order to achieve a desired polymer was performed successfully. The obtained polymers under different conditions were evaluated for the potential application as a hemostatic agent, including tests of swelling behavior in different fluids, whole-blood clotting time and enzymatic bio-degradation. Results have shown that the citric acid/polysaccharides ratio of 0.01 produced a comparative yield of polymer flakes with increased swelling properties, as well as shortened in vitro blood clotting time and gradual biodegradability in three experimental days. Therefore, the citric acid/polysaccharides ratio of 0.01 was selected for the subsequent experiment to obtain the polymer after 1.5 h of purification, which gave the best swelling properties, 20 times the initial weight, reduced the whole-blood clotting time by 50% and showed a rapid bio-degradation. The results obtained from this study provide essential knowledge on the research of the use of S. foetida gum polysaccharides as a passive hemostatic agent, thereby extending the potential pharmaceutical applications of natural polysaccharides in Vietnam.
{"title":"Synthesis of cross-linked polymer based on purified Sterculia foetida L. gum as a potential hemostatic agent","authors":"X. Le, T. Tong, Thanh Tung Le, Pham C. T. Dung, L. Bach","doi":"10.1177/08839115221143443","DOIUrl":"https://doi.org/10.1177/08839115221143443","url":null,"abstract":"A method for purifying the polysaccharides from Sterculia foetida L. gum was developed. The effects of purifying conditions were also studied. Results have shown that the conditions of pH 0.5, temperature of 80°C, and 2.5-h reaction duration gave the best polysaccharides yield. The mineral content and the molecular weight of the obtained polysaccharides were measured for characterization. The cross-linking of the polysaccharides with citric acid in order to achieve a desired polymer was performed successfully. The obtained polymers under different conditions were evaluated for the potential application as a hemostatic agent, including tests of swelling behavior in different fluids, whole-blood clotting time and enzymatic bio-degradation. Results have shown that the citric acid/polysaccharides ratio of 0.01 produced a comparative yield of polymer flakes with increased swelling properties, as well as shortened in vitro blood clotting time and gradual biodegradability in three experimental days. Therefore, the citric acid/polysaccharides ratio of 0.01 was selected for the subsequent experiment to obtain the polymer after 1.5 h of purification, which gave the best swelling properties, 20 times the initial weight, reduced the whole-blood clotting time by 50% and showed a rapid bio-degradation. The results obtained from this study provide essential knowledge on the research of the use of S. foetida gum polysaccharides as a passive hemostatic agent, thereby extending the potential pharmaceutical applications of natural polysaccharides in Vietnam.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83546650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-07DOI: 10.1177/08839115221138777
Esra Pilavci, Musa Ayran, Dilay Ulubay, E. Kaya, G. Tinaz, O. Bingol Ozakpinar, A. Sancakli, O. Gunduz
In the present study, the effect of different ratios of GelMA concentration has been exhibited for wound dressing implementation by the electrospinning method using a new polymer combination of Gelatin methacrylate (GelMA)/Polycaprolactone (PCL)/Chitosan (CS). The nanofiber composites were fabricated due to their biocompatible, biodegradable, improved mechanical strength, low degradation rate, and hydrophilic nature to develop cell-mimicking, cell adhesion, proliferation, and differentiation. Different concentrations of GelMA were added to the PCL/CS solution as 5, 10, and 20 wt%, respectively, in the formic acid/acetic acid (7:3) solution. A photoinitiator was added to the solution for photo-crosslinking of GelMA. The influence of different solution concentrations (5, 10, and 20 wt%) on the structure’s nanofiber production and fiber morphology was examined. SEM micrographs revealed that varied GelMA concentrations resulted in suitable and stable nanofiber composites. The average diameter of nanofiber composites grows as the GelMA concentration rises. FTIR, DSC, tensile test, degradation, and swelling test were evaluated. The results demonstrated that high mechanical strength, hydrophilic properties, and a slow degradation rate were observed with the presence and increment of GelMA concentration within the nanofiber composites. The antibacterial potential of GelMA/PCL/CS nanofiber composites was evaluated against P. aeruginosa and S. aureus using a disc diffusion assay. In vitro cell culture research was conducted by seeding NIH 3T3 fibroblast cells on nanofiber composites, proving these cells’ high cell proliferation rate, viability, and adhesion. 10 wt% GelMA-based nanofiber composites were found to have great potential for wound dressing applications.
{"title":"Fabrication and characterization of electrospun GelMA/PCL/CS nanofiber composites for wound dressing applications","authors":"Esra Pilavci, Musa Ayran, Dilay Ulubay, E. Kaya, G. Tinaz, O. Bingol Ozakpinar, A. Sancakli, O. Gunduz","doi":"10.1177/08839115221138777","DOIUrl":"https://doi.org/10.1177/08839115221138777","url":null,"abstract":"In the present study, the effect of different ratios of GelMA concentration has been exhibited for wound dressing implementation by the electrospinning method using a new polymer combination of Gelatin methacrylate (GelMA)/Polycaprolactone (PCL)/Chitosan (CS). The nanofiber composites were fabricated due to their biocompatible, biodegradable, improved mechanical strength, low degradation rate, and hydrophilic nature to develop cell-mimicking, cell adhesion, proliferation, and differentiation. Different concentrations of GelMA were added to the PCL/CS solution as 5, 10, and 20 wt%, respectively, in the formic acid/acetic acid (7:3) solution. A photoinitiator was added to the solution for photo-crosslinking of GelMA. The influence of different solution concentrations (5, 10, and 20 wt%) on the structure’s nanofiber production and fiber morphology was examined. SEM micrographs revealed that varied GelMA concentrations resulted in suitable and stable nanofiber composites. The average diameter of nanofiber composites grows as the GelMA concentration rises. FTIR, DSC, tensile test, degradation, and swelling test were evaluated. The results demonstrated that high mechanical strength, hydrophilic properties, and a slow degradation rate were observed with the presence and increment of GelMA concentration within the nanofiber composites. The antibacterial potential of GelMA/PCL/CS nanofiber composites was evaluated against P. aeruginosa and S. aureus using a disc diffusion assay. In vitro cell culture research was conducted by seeding NIH 3T3 fibroblast cells on nanofiber composites, proving these cells’ high cell proliferation rate, viability, and adhesion. 10 wt% GelMA-based nanofiber composites were found to have great potential for wound dressing applications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86168031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Unlike the central nervous system (CNS), peripheral nervous system (PNS) injuries are partially repairable. Nerve guidance channels (NGCs) have been shown to improve the level of nerve repair after injury. In the present study, we developed a nanofiber NGC for the delivery of acetyl L carnitine (ALC) in a rat model of sciatic nerve injury. NGCs were produced by electrospinning a polymer blend of polycaprolacton and gelatin. The physicochemical and biological properties of developed scaffolds were investigated using Scanning electron microscopy, surface hydrophilicity measurement, porosity measurement, tensile strength studies, cell viability assay, and cell attachment assay. ALC was included in the collagen hydrogels at three weight ratios of 1%, 3%, and 5%. Cell viability assay showed that the hydrogels containing 5% ALC demonstrated a more favorable effect on PC-12 metabolic activity. Therefore, this concentration was chosen to treat PNS injury. The NGCs were implanted in rats and then their lumen was filled with collagen hydrogel + 5%ALC. The results of histopathological examinations and functional recovery studies showed that NGCs filled with ALC containing hydrogel have significant recovery potential compared to NGCs loaded with collagen hydrogels without ALC. Our results support the potential use of ALC-delivering NGCs in the treatment of peripheral nerve injury in the clinic.
{"title":"Regeneration of sciatic nerve injury through nanofiber neural guidance channels containing collagen hydrogel and acetyl L carnitine: An in vitro and in vivo study","authors":"Gholamreza Savari Kouzehkonan, Negar Motakef Kazemi, Mahdi Adabi, Seyyedeh Elaheh Mosavi, Seyed Mahdi Rezayat Sorkhabadi","doi":"10.1177/08839115221137654","DOIUrl":"https://doi.org/10.1177/08839115221137654","url":null,"abstract":"Unlike the central nervous system (CNS), peripheral nervous system (PNS) injuries are partially repairable. Nerve guidance channels (NGCs) have been shown to improve the level of nerve repair after injury. In the present study, we developed a nanofiber NGC for the delivery of acetyl L carnitine (ALC) in a rat model of sciatic nerve injury. NGCs were produced by electrospinning a polymer blend of polycaprolacton and gelatin. The physicochemical and biological properties of developed scaffolds were investigated using Scanning electron microscopy, surface hydrophilicity measurement, porosity measurement, tensile strength studies, cell viability assay, and cell attachment assay. ALC was included in the collagen hydrogels at three weight ratios of 1%, 3%, and 5%. Cell viability assay showed that the hydrogels containing 5% ALC demonstrated a more favorable effect on PC-12 metabolic activity. Therefore, this concentration was chosen to treat PNS injury. The NGCs were implanted in rats and then their lumen was filled with collagen hydrogel + 5%ALC. The results of histopathological examinations and functional recovery studies showed that NGCs filled with ALC containing hydrogel have significant recovery potential compared to NGCs loaded with collagen hydrogels without ALC. Our results support the potential use of ALC-delivering NGCs in the treatment of peripheral nerve injury in the clinic.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79297053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.1177/08839115221138772
J. Sebastian, Jhancy Mary Samuel
Breast cancer in women is amongst the most significant concerns from time immemorial in the field of oncology. This study proposes an anticancerous polymeric material based on an electroactive substituted polyaniline blend, poly(2-aminobenzoic acid)-blend-Aloe vera (PABA/AV) synthesized by the emulsion polymerization method. The structural, thermal, and morphological characteristics determined using FT-IR and UV-Visible Spectroscopy, XRD, TGA, DTA, and SEM-EDX validated the thermally stable, semi-crystalline, emeraldine salt structure. The material is semi-conducting, and the electrical conductivity measured is 1.86 × 10−3 S/cm. It shows bactericidal efficacy against Enterococcus faecalis at a minimum inhibitory and minimum bactericidal concentration of 50 μg/mL. The radical cations in the emeraldine polymer chain reduce the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and exhibit a significant % of DPPH scavenging (89.85%) at 20 μL. The polymer blend is active against the human breast cancer cell line MDA-MB-231 and causes 78.65% cytotoxicity at a concentration of 125 μg/mL. The synergistic effect of the ancient healing Aloe vera plant and the electroactive biocompatible poly(2-aminobenzoic acid) certainly opens up new developments in the field of cancer therapy.
{"title":"Anticancer potential of poly(2-aminobenzoic acid)-blend-Aloe vera against the human breast cancer cell line MDA-MB-231","authors":"J. Sebastian, Jhancy Mary Samuel","doi":"10.1177/08839115221138772","DOIUrl":"https://doi.org/10.1177/08839115221138772","url":null,"abstract":"Breast cancer in women is amongst the most significant concerns from time immemorial in the field of oncology. This study proposes an anticancerous polymeric material based on an electroactive substituted polyaniline blend, poly(2-aminobenzoic acid)-blend-Aloe vera (PABA/AV) synthesized by the emulsion polymerization method. The structural, thermal, and morphological characteristics determined using FT-IR and UV-Visible Spectroscopy, XRD, TGA, DTA, and SEM-EDX validated the thermally stable, semi-crystalline, emeraldine salt structure. The material is semi-conducting, and the electrical conductivity measured is 1.86 × 10−3 S/cm. It shows bactericidal efficacy against Enterococcus faecalis at a minimum inhibitory and minimum bactericidal concentration of 50 μg/mL. The radical cations in the emeraldine polymer chain reduce the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and exhibit a significant % of DPPH scavenging (89.85%) at 20 μL. The polymer blend is active against the human breast cancer cell line MDA-MB-231 and causes 78.65% cytotoxicity at a concentration of 125 μg/mL. The synergistic effect of the ancient healing Aloe vera plant and the electroactive biocompatible poly(2-aminobenzoic acid) certainly opens up new developments in the field of cancer therapy.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78952740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-23DOI: 10.1177/08839115221138773
Manjushree Hk, Prakruti P Acharya, G. Bhat, S. More, Aneesa Fasim
The present study was conducted to examine the bioactive and wound healing properties of collagen hydrolysate derived from Piaractus brachypomus (pacu) fish skin waste. Collagen type I (P coll.) yielding 72.25% was isolated from skin waste by following acid-soluble collagen extraction method. Further, collagen was fragmented using bacterial collagenase and the processed collagen hydrolysate (peptides) was in the range of 10–15 kDa that was further purified using ion-exchange chromatography. The FTIR spectra of both P coll. and collagen hydrolysate (PSCH) were nearly similar showing that PSCH retained the structural and chemical composition similar to its parent molecule (P coll.). Solubility analysis revealed that PSCH has slightly better solubility compared to P coll. Similarly, scanning electron micrographs also exhibited more uniform and porous microstructure of PSCH compared to P coll. Further, PSCH was found to be efficient in peroxide quenching (64.5%) and radical scavenging activities (85.74%). MTT studies confirmed PSCH to be non-toxic displaying 84.68% cell viability at the highest concentration (3 mg/ml) and hemocompatibility test revealed PSCH to be non-hemolytic with minimal lysis of only 2.1% of human RBCs. In addition, PSCH also displayed a remarkable wound closure ability of more than 80% at 12 h and 100% within 24 h. Hence, these findings suggest that recycled PSCH has potent wound healing ability and can be produced economically on a large scale for possible biological applications in regenerative medicine.
{"title":"Biophysical and in vitro wound healing assessment of collagen peptides processed from fish skin waste","authors":"Manjushree Hk, Prakruti P Acharya, G. Bhat, S. More, Aneesa Fasim","doi":"10.1177/08839115221138773","DOIUrl":"https://doi.org/10.1177/08839115221138773","url":null,"abstract":"The present study was conducted to examine the bioactive and wound healing properties of collagen hydrolysate derived from Piaractus brachypomus (pacu) fish skin waste. Collagen type I (P coll.) yielding 72.25% was isolated from skin waste by following acid-soluble collagen extraction method. Further, collagen was fragmented using bacterial collagenase and the processed collagen hydrolysate (peptides) was in the range of 10–15 kDa that was further purified using ion-exchange chromatography. The FTIR spectra of both P coll. and collagen hydrolysate (PSCH) were nearly similar showing that PSCH retained the structural and chemical composition similar to its parent molecule (P coll.). Solubility analysis revealed that PSCH has slightly better solubility compared to P coll. Similarly, scanning electron micrographs also exhibited more uniform and porous microstructure of PSCH compared to P coll. Further, PSCH was found to be efficient in peroxide quenching (64.5%) and radical scavenging activities (85.74%). MTT studies confirmed PSCH to be non-toxic displaying 84.68% cell viability at the highest concentration (3 mg/ml) and hemocompatibility test revealed PSCH to be non-hemolytic with minimal lysis of only 2.1% of human RBCs. In addition, PSCH also displayed a remarkable wound closure ability of more than 80% at 12 h and 100% within 24 h. Hence, these findings suggest that recycled PSCH has potent wound healing ability and can be produced economically on a large scale for possible biological applications in regenerative medicine.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86882464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-19DOI: 10.1177/08839115221126714
Mahtab Doostan, Maryam Doostan, H. Maleki, R. Faridi Majidi, Fariba Bagheri, H. Ghanbari
Fabrication of a biocompatible nanofibrous dressing with the advantage of the inclusion of bioactive herbal extracts is a promising approach in skin tissue engineering and wound healing applications. Herbal extracts possess many properties to promote the wound healing process, such as antioxidant properties, anti-inflammation activities as well as enhancing fibroblasts proliferation and migration. In this study, Calendula officinalis (C. officinalis) and coffee extracts were loaded into poly(vinyl alcohol)/poly(ɛ-caprolactone) (PVA/PCL) nanofibrous mats. The obtained scaffolds were then characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), contact angle, and mechanical measurements. Also, the antioxidant activity, scratch assay, and cell viability of fibroblast cells were also evaluated. The results showed PVA/PCL scaffold loaded with 10 wt% C. officinalis and coffee extracts displayed smooth homogenous morphology with 317 ± 106 nm average diameter. Moreover, the relevant analyses confirmed that the extracts were incorporated into the nanofibers with suitable hydrophilicity and higher mechanical strength (4 ± 0.4 MPa). The antioxidant assay showed that IC50 values of coffee and C. officinalis extracts were 46 ± 1 ppm and 101 ± 4 ppm, successively, which presented a high antioxidant activity. The combination of both extracts showed a higher rate of migration than individual extracts with not detected cytotoxic effects on the human dermal fibroblast cells. In conclusion, our results confirmed that the coffee and C. officinalis extracts loaded PVA/PCL nanofibrous scaffolds could provide an appropriate construct for wound healing applications.
{"title":"Co-electrospun poly(vinyl alcohol)/poly(ɛ-caprolactone) nanofiber scaffolds containing coffee and Calendula officinalis extracts for wound healing applications","authors":"Mahtab Doostan, Maryam Doostan, H. Maleki, R. Faridi Majidi, Fariba Bagheri, H. Ghanbari","doi":"10.1177/08839115221126714","DOIUrl":"https://doi.org/10.1177/08839115221126714","url":null,"abstract":"Fabrication of a biocompatible nanofibrous dressing with the advantage of the inclusion of bioactive herbal extracts is a promising approach in skin tissue engineering and wound healing applications. Herbal extracts possess many properties to promote the wound healing process, such as antioxidant properties, anti-inflammation activities as well as enhancing fibroblasts proliferation and migration. In this study, Calendula officinalis (C. officinalis) and coffee extracts were loaded into poly(vinyl alcohol)/poly(ɛ-caprolactone) (PVA/PCL) nanofibrous mats. The obtained scaffolds were then characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), contact angle, and mechanical measurements. Also, the antioxidant activity, scratch assay, and cell viability of fibroblast cells were also evaluated. The results showed PVA/PCL scaffold loaded with 10 wt% C. officinalis and coffee extracts displayed smooth homogenous morphology with 317 ± 106 nm average diameter. Moreover, the relevant analyses confirmed that the extracts were incorporated into the nanofibers with suitable hydrophilicity and higher mechanical strength (4 ± 0.4 MPa). The antioxidant assay showed that IC50 values of coffee and C. officinalis extracts were 46 ± 1 ppm and 101 ± 4 ppm, successively, which presented a high antioxidant activity. The combination of both extracts showed a higher rate of migration than individual extracts with not detected cytotoxic effects on the human dermal fibroblast cells. In conclusion, our results confirmed that the coffee and C. officinalis extracts loaded PVA/PCL nanofibrous scaffolds could provide an appropriate construct for wound healing applications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2022-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86482733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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