Pub Date : 2023-12-07DOI: 10.1177/08839115231216998
Gulsah Yildiz, Y. E. Arslan, Burak Derkuş, Billur Sezgin, Yusuf Ziya Menceloglu, G. R. Bayar
Tissue-engineered skin substitutes have great potential to treat chronic wounds and high-degree burns. Existing solutions, such as Integra Dermal Template, are extensively used for skin defects. However, these templates are still lacking in terms of recreating the functionality of the native tissue and providing scarless healing. In this study, polycaprolactone/silk fibroin (PCL/SF)-based nanofibers with varying blends were fabricated and characterized to develop a novel skin substitute. Morphological analysis showed that the nanofiber distribution of each sample was homogenous without showing any beads. In terms of mechanical properties, all the samples other than SF showed sufficient mechanical strength. It was observed that adding a specific amount of SF into the PCL nanofiber improves the tensile strength of the samples due to the introduction of intermolecular interactions from the functional groups of SF. In addition, incorporating SF into PCL improved Young’s modulus of the PCL nanofibers since SF provides stiffness and structural integrity to the overall structure. Water contact angle analysis was performed as the hydrophilicity of a biomaterial is a significant factor in cell functionality. Each sample had a contact angle between 33° and 48°, indicating the adequate hydrophilicity of nanofibers for advanced cell proliferation other than PCL. Cell proliferation and viability studies were conducted with the seeding of primary human keratinocytes on the samples. It was examined that scaffolds containing blends of PCL and SF resulted in higher cell proliferation and viability after 7 days compared to pure PCL and SF nanofibers.
{"title":"Development and characterization of skin substitutes from electrospun polycaprolactone/silk fibroin","authors":"Gulsah Yildiz, Y. E. Arslan, Burak Derkuş, Billur Sezgin, Yusuf Ziya Menceloglu, G. R. Bayar","doi":"10.1177/08839115231216998","DOIUrl":"https://doi.org/10.1177/08839115231216998","url":null,"abstract":"Tissue-engineered skin substitutes have great potential to treat chronic wounds and high-degree burns. Existing solutions, such as Integra Dermal Template, are extensively used for skin defects. However, these templates are still lacking in terms of recreating the functionality of the native tissue and providing scarless healing. In this study, polycaprolactone/silk fibroin (PCL/SF)-based nanofibers with varying blends were fabricated and characterized to develop a novel skin substitute. Morphological analysis showed that the nanofiber distribution of each sample was homogenous without showing any beads. In terms of mechanical properties, all the samples other than SF showed sufficient mechanical strength. It was observed that adding a specific amount of SF into the PCL nanofiber improves the tensile strength of the samples due to the introduction of intermolecular interactions from the functional groups of SF. In addition, incorporating SF into PCL improved Young’s modulus of the PCL nanofibers since SF provides stiffness and structural integrity to the overall structure. Water contact angle analysis was performed as the hydrophilicity of a biomaterial is a significant factor in cell functionality. Each sample had a contact angle between 33° and 48°, indicating the adequate hydrophilicity of nanofibers for advanced cell proliferation other than PCL. Cell proliferation and viability studies were conducted with the seeding of primary human keratinocytes on the samples. It was examined that scaffolds containing blends of PCL and SF resulted in higher cell proliferation and viability after 7 days compared to pure PCL and SF nanofibers.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"28 7","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138594471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-06DOI: 10.1177/08839115231216997
M. A. A. Fraga, C. S. Garfias, Patricia Akemi Nishitani Shibasaki, A. Correr, M. D. De Goes, R. Puppin-Rontani
To identify the effectiveness of polymer-induced liquid precursors (PILP) on dentin remineralization and the assessment approaches used to evaluate remineralization. The analyses were done into six stages: (1) Identifying the research question; (2) Identifying the studies; (3) Selecting relevant studies; (4) Plotting the data; (5) Reporting results; and (6) Risk of bias. The searches were carried out in the following databases: PubMed, Web of Science, and Scopus. A total of 247 articles were identified in the electronic database search. After applying the eligibility criteria, only 12 articles were included for data extraction. The outcomes of dentin remineralization patterns were plotted in association to PILP agent type and application mode. The intrafibrillar mineralization occurred in 78% of studies where polyaspartic acid (pAsp) was used, and 50% when the polyacrylic acid (PAA) was used. All the studies where PILP was used in a restorative material (resin composite, glass ionomer, and adhesive system)—extra and intrafibrillar mineralization pattern was found. The association of PILP agents with other materials with potential for mineralization showed beneficial results in remineralization, since they provide a medium to high concentration of calcium and phosphate. The pAsp showed better results for interfibrillar remineralization when compared to PAA. The association with Ca/P release materials or solution Ca P are essentials for mineralization via PILP agent. Further studies are needed to assess the effectiveness of remineralization through PILP agents because the level of evidence of the studies was low.
探讨聚合物诱导的液体前体(PILP)对牙本质再矿化的影响及其评价方法。分析分为六个阶段:(1)确定研究问题;(2)确定研究;(3)选择相关研究;(4)绘制数据;(五)报告结果;(6)偏倚风险。检索在以下数据库中进行:PubMed, Web of Science和Scopus。电子数据库检索共检索到247篇论文。应用资格标准后，仅纳入12篇文章进行数据提取。牙本质再矿化模式的结果与PILP药物类型和应用方式有关。在使用聚天冬氨酸(pAsp)的研究中，78%的研究发生了纤维内矿化，而在使用聚丙烯酸(PAA)的研究中，这一比例为50%。所有将PILP用于修复材料(树脂复合材料、玻璃离聚体和粘合剂系统)的研究都发现了纤维外和纤维内矿化模式。PILP剂与其他具有矿化潜力的材料的结合在再矿化中显示出有益的结果，因为它们提供了中等到高浓度的钙和磷酸盐。与PAA相比，pAsp在纤维间再矿化方面表现出更好的效果。与Ca/P释放物质或Ca - P溶液的结合是通过PILP剂矿化的必要条件。由于这些研究的证据水平较低，需要进一步的研究来评估通过PILP药物进行再矿化的有效性。
{"title":"Polymer-induced liquid precursors: How are they being used and what is the effect on dentin remineralization? A scoping review","authors":"M. A. A. Fraga, C. S. Garfias, Patricia Akemi Nishitani Shibasaki, A. Correr, M. D. De Goes, R. Puppin-Rontani","doi":"10.1177/08839115231216997","DOIUrl":"https://doi.org/10.1177/08839115231216997","url":null,"abstract":"To identify the effectiveness of polymer-induced liquid precursors (PILP) on dentin remineralization and the assessment approaches used to evaluate remineralization. The analyses were done into six stages: (1) Identifying the research question; (2) Identifying the studies; (3) Selecting relevant studies; (4) Plotting the data; (5) Reporting results; and (6) Risk of bias. The searches were carried out in the following databases: PubMed, Web of Science, and Scopus. A total of 247 articles were identified in the electronic database search. After applying the eligibility criteria, only 12 articles were included for data extraction. The outcomes of dentin remineralization patterns were plotted in association to PILP agent type and application mode. The intrafibrillar mineralization occurred in 78% of studies where polyaspartic acid (pAsp) was used, and 50% when the polyacrylic acid (PAA) was used. All the studies where PILP was used in a restorative material (resin composite, glass ionomer, and adhesive system)—extra and intrafibrillar mineralization pattern was found. The association of PILP agents with other materials with potential for mineralization showed beneficial results in remineralization, since they provide a medium to high concentration of calcium and phosphate. The pAsp showed better results for interfibrillar remineralization when compared to PAA. The association with Ca/P release materials or solution Ca P are essentials for mineralization via PILP agent. Further studies are needed to assess the effectiveness of remineralization through PILP agents because the level of evidence of the studies was low.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"49 7","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138597522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Developing wound dressings with a high potential to cover damaged skin tissue and facilitate cell adhesion and migration at the injury site is crucial in skin tissue engineering to accelerate wound healing. Electrospun nanofibers from natural/synthetic polymers are amongst the favorable wound dressings with appropriate physicochemical and biological properties. As well, nanoformulations of phenolic phytochemical “eugenol” have been shown to fasten wound healing via various anti-inflammatory and anti-oxidant effects. Herein, we developed a bi-component wound dressing of PCL/Cs electrospun nanofibers and eugenol nanogel to investigate its effects on tissue healing in vivo. PCL/Cs nanofibers were fabricated using an electrospinning method at the 15:1 ratio, and eugenol-loaded nanogels were synthesized by adding carboxymethylcellulose as the gelling agent, and their physicochemical characteristics were assessed. Scaffolds were implanted in a full-thickness excision wound model in Wistar rats, followed up for 21 days. The results showed that electrospun nanofibers had an average diameter of 228 nm with uniform and smooth morphology aligned randomly. Eugenol-loaded nanogel showed an average size distribution of 126 nm. Eugenol-loaded nanogel and nanogel + nanofiber groups significantly reduced wound surface area over 21 days. Histological evaluations showed that Eugenol-loaded nanogel and nanogel + nanofiber groups developed the full-thickness epidermis with the complete epithelium and stratum corneum, angiogenesis, and low macrophage infiltration in which predominantly mature collagen fibers were poorly and well organized, respectively. The combination of eugenol-loaded nanogel + PCL/Cs nanofiber accelerated wound healing by reducing inflammation, and edema along with enhancing angiogenesis, collagen synthesis, and re-epithelialization.
{"title":"Fabrication, characterization, and in vivo implantation of eugenol-loaded nanogels and PCL/Cs electrospun nanofibers for wound healing applications","authors":"Fariba Noori, Mahmoud Osanloo, Hamid Reza Moradi, Hamidreza Ghaderi Jafarbeigloo, Mozhgan Jirehnezhadyan, Seyed Amin Kouhpayeh, Mahtab Tirgare, Azam Bozorgi, Arash Goodarzi","doi":"10.1177/08839115231207337","DOIUrl":"https://doi.org/10.1177/08839115231207337","url":null,"abstract":"Developing wound dressings with a high potential to cover damaged skin tissue and facilitate cell adhesion and migration at the injury site is crucial in skin tissue engineering to accelerate wound healing. Electrospun nanofibers from natural/synthetic polymers are amongst the favorable wound dressings with appropriate physicochemical and biological properties. As well, nanoformulations of phenolic phytochemical “eugenol” have been shown to fasten wound healing via various anti-inflammatory and anti-oxidant effects. Herein, we developed a bi-component wound dressing of PCL/Cs electrospun nanofibers and eugenol nanogel to investigate its effects on tissue healing in vivo. PCL/Cs nanofibers were fabricated using an electrospinning method at the 15:1 ratio, and eugenol-loaded nanogels were synthesized by adding carboxymethylcellulose as the gelling agent, and their physicochemical characteristics were assessed. Scaffolds were implanted in a full-thickness excision wound model in Wistar rats, followed up for 21 days. The results showed that electrospun nanofibers had an average diameter of 228 nm with uniform and smooth morphology aligned randomly. Eugenol-loaded nanogel showed an average size distribution of 126 nm. Eugenol-loaded nanogel and nanogel + nanofiber groups significantly reduced wound surface area over 21 days. Histological evaluations showed that Eugenol-loaded nanogel and nanogel + nanofiber groups developed the full-thickness epidermis with the complete epithelium and stratum corneum, angiogenesis, and low macrophage infiltration in which predominantly mature collagen fibers were poorly and well organized, respectively. The combination of eugenol-loaded nanogel + PCL/Cs nanofiber accelerated wound healing by reducing inflammation, and edema along with enhancing angiogenesis, collagen synthesis, and re-epithelialization.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"63 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135566899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hybrid crosslinked hydrogel-based drug delivery systems can deliver therapeutically beneficial drug release effects through controlled release on a temporal and spatial scale. Due to their tuneable physicochemical properties and the ability to prevent drug degradation, these systems facilitate different physicochemical interactions that control drug release. This research aims to synthesize a potent pH-sensitive crosslinked chitosan/polyvinylpyrrolidone hydrogel using different ratios of both the polymers and varying the glutaraldehyde crosslinking agent amount by the solution casting technique for controlled amoxicillin-release properties to mitigate gastrointestinal tract bacterial infection. The effect of polymer ratios and glutaraldehyde amount is investigated by the developing porosity, gel fraction, and extent of swelling in simulated physiological fluids of pH 1.2, 6.8, and 7.4 and in vitro biodegradation at pH 7.4. Interaction between the polymers with the formation of crosslinked structures, good stability, amorphous phase nature, and honeycomb-like structures of the hydrogels is revealed by Fourier-transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. Based on these properties, a chitosan/polyvinylpyrrolidone hydrogel (ratio of 60:40) crosslinked with 600 μL glutaraldehyde in 8 g of the polymers displaying adequate swelling in acidic to basic pH and in vitro biodegradation at pH 7.4 is chosen for the in situ loading of 200 mg of the drug amoxicillin. The cumulative drug release in simulated physiological fluids and the drug release kinetics using different models show that the best-fit Korsmeyer-Peppas model suggests amoxicillin release from the matrix follows diffusion and swelling-controlled time-dependent non-Fickian transport related to hydrogel erosion. This composition displays excellent antimicrobial activity against Streptococcus pyogenes and Escherichia coli.
{"title":"Synthesis and characterization of chitosan/polyvinylpyrrolidone hydrogels for controlled amoxicillin release","authors":"Zerihun Feyisa, Neeraj K Gupta, Gemechu Deressa Edossa, Anandhakumar Sundaramurthy, Ashish Kapoor","doi":"10.1177/08839115231207817","DOIUrl":"https://doi.org/10.1177/08839115231207817","url":null,"abstract":"Hybrid crosslinked hydrogel-based drug delivery systems can deliver therapeutically beneficial drug release effects through controlled release on a temporal and spatial scale. Due to their tuneable physicochemical properties and the ability to prevent drug degradation, these systems facilitate different physicochemical interactions that control drug release. This research aims to synthesize a potent pH-sensitive crosslinked chitosan/polyvinylpyrrolidone hydrogel using different ratios of both the polymers and varying the glutaraldehyde crosslinking agent amount by the solution casting technique for controlled amoxicillin-release properties to mitigate gastrointestinal tract bacterial infection. The effect of polymer ratios and glutaraldehyde amount is investigated by the developing porosity, gel fraction, and extent of swelling in simulated physiological fluids of pH 1.2, 6.8, and 7.4 and in vitro biodegradation at pH 7.4. Interaction between the polymers with the formation of crosslinked structures, good stability, amorphous phase nature, and honeycomb-like structures of the hydrogels is revealed by Fourier-transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. Based on these properties, a chitosan/polyvinylpyrrolidone hydrogel (ratio of 60:40) crosslinked with 600 μL glutaraldehyde in 8 g of the polymers displaying adequate swelling in acidic to basic pH and in vitro biodegradation at pH 7.4 is chosen for the in situ loading of 200 mg of the drug amoxicillin. The cumulative drug release in simulated physiological fluids and the drug release kinetics using different models show that the best-fit Korsmeyer-Peppas model suggests amoxicillin release from the matrix follows diffusion and swelling-controlled time-dependent non-Fickian transport related to hydrogel erosion. This composition displays excellent antimicrobial activity against Streptococcus pyogenes and Escherichia coli.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"242 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135371139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
When 3D silk fibroin scaffolds are used for the regeneration of soft tissues with fast regeneration rates, such as skin dermis, one concern is to accelerate the biodegradation of scaffolds and to match the degradation rate of scaffolds with the regeneration rate of tissues. In this study, sericin was incorporated into 3D silk fibroin scaffolds through crosslinking and followed by freeze-drying. The effects of incorporating sericin on the pore wall microstructure within the scaffolds, the biodegradability of scaffolds and cell proliferation within scaffolds were investigated. It was found that a large number of secondary pores and nanoscale particles were generated on the pore walls within the scaffolds due to the incorporation of sericin and that the number of secondary pores and the size of the particles increased with increasing sericin proportion. The results of in vitro biodegradation and coculture with human umbilical vein vascular endothelial cells demonstrated that the incorporation of sericin not only significantly accelerated the degradation of 3D silk fibroin scaffolds, but also promoted cell adhesion and proliferation. The secondary pores and particles generated on the pore walls inside the fibroin/sericin hybrid scaffolds had a positive contribution to promoting cell adhesion and proliferation. This study provides a biocompatible method for the modification of silk fibroin scaffolds, which can not only accelerate the biodegradation of the scaffold but also promote the adhesion and proliferation of seeded cells.
{"title":"A silk fibroin scaffold that accelerates both biodegradation and cell proliferation by incorporating sericin","authors":"Wenjing Zhang, Xiaoxiao Sun, Peng Pan, Xueping Liu, Cheng Hu, Mengmeng Wang, Mingzhong Li, Yu Liu","doi":"10.1177/08839115231207815","DOIUrl":"https://doi.org/10.1177/08839115231207815","url":null,"abstract":"When 3D silk fibroin scaffolds are used for the regeneration of soft tissues with fast regeneration rates, such as skin dermis, one concern is to accelerate the biodegradation of scaffolds and to match the degradation rate of scaffolds with the regeneration rate of tissues. In this study, sericin was incorporated into 3D silk fibroin scaffolds through crosslinking and followed by freeze-drying. The effects of incorporating sericin on the pore wall microstructure within the scaffolds, the biodegradability of scaffolds and cell proliferation within scaffolds were investigated. It was found that a large number of secondary pores and nanoscale particles were generated on the pore walls within the scaffolds due to the incorporation of sericin and that the number of secondary pores and the size of the particles increased with increasing sericin proportion. The results of in vitro biodegradation and coculture with human umbilical vein vascular endothelial cells demonstrated that the incorporation of sericin not only significantly accelerated the degradation of 3D silk fibroin scaffolds, but also promoted cell adhesion and proliferation. The secondary pores and particles generated on the pore walls inside the fibroin/sericin hybrid scaffolds had a positive contribution to promoting cell adhesion and proliferation. This study provides a biocompatible method for the modification of silk fibroin scaffolds, which can not only accelerate the biodegradation of the scaffold but also promote the adhesion and proliferation of seeded cells.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"240 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135371154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.1177/08839115231207193
{"title":"Corrigendum to “Vancomycin coupled chitosan/PEO nanofibrous scaffold with the desired antibacterial activity as a potential for biomedical application”","authors":"","doi":"10.1177/08839115231207193","DOIUrl":"https://doi.org/10.1177/08839115231207193","url":null,"abstract":"","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136341440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-25DOI: 10.1177/08839115231199700
Yasir Iqbal, Shahzad Ali Shahid Chatha, Zunera Chauhdary, Abdullah Ijaz Hussain, Ikram Ullah Khan
The study aimed to evaluate moringa gum (MG) and carboxymethyl moringa (CMG) for their possible use in hydrogel-based dressings to treat cutaneous wounds. Initially, MG was characterized for its physical, functional, morphological, and thermal properties. The MG and CMG have shown limited toxicity as indicated by hemolytic assay where values were less than 2.5%. Furthermore, these samples also showed antimicrobial potential. The antioxidant potential of MG was slightly greater than its carboxymethyl derivative. Afterward, MG and CMG were encapsulated in sodium alginate-based hydrogel films. These films showed good physical attributes necessary for the end application. These films were tested in an open wound model in rats. The MG containing films showed the highest % wound contraction on the 10th day, that is,79.42 ± 0.46 with low levels of IL6 and TNF alfa. Out of MG and CMG films, the native moringa gum-based thin film showed comparatively higher potential for wound healing and in the future could be a potential candidate for wound healing dressings.
{"title":"Design and evaluation of moringa gum-based hydrogel dressings for cutaneous wound healing","authors":"Yasir Iqbal, Shahzad Ali Shahid Chatha, Zunera Chauhdary, Abdullah Ijaz Hussain, Ikram Ullah Khan","doi":"10.1177/08839115231199700","DOIUrl":"https://doi.org/10.1177/08839115231199700","url":null,"abstract":"The study aimed to evaluate moringa gum (MG) and carboxymethyl moringa (CMG) for their possible use in hydrogel-based dressings to treat cutaneous wounds. Initially, MG was characterized for its physical, functional, morphological, and thermal properties. The MG and CMG have shown limited toxicity as indicated by hemolytic assay where values were less than 2.5%. Furthermore, these samples also showed antimicrobial potential. The antioxidant potential of MG was slightly greater than its carboxymethyl derivative. Afterward, MG and CMG were encapsulated in sodium alginate-based hydrogel films. These films showed good physical attributes necessary for the end application. These films were tested in an open wound model in rats. The MG containing films showed the highest % wound contraction on the 10th day, that is,79.42 ± 0.46 with low levels of IL6 and TNF alfa. Out of MG and CMG films, the native moringa gum-based thin film showed comparatively higher potential for wound healing and in the future could be a potential candidate for wound healing dressings.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135815691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-20DOI: 10.1177/08839115231198528
Özlem Erdal Altıntaş, Pınar Aytar Çelik
Hydrogels in active wound care products are 3D polymeric scaffolds that provide the desired moisture balance in the wound area with their high water retention capacity. Thanks to their biocompatible properties, flexible and porous structures, and high hydrophilic properties, they have frequently been preferred in the repair and regeneration of cells and tissues and the controlled release of bioactive substances to the target area. In this study, hydrogel structures were developed for the first time with levan produced by Halomonas elongata 153B halophilic bacteria. Extracts from Plantago lanceolata L., commonly known as a wound herb, which has medicinal importance for cell regeneration, ensuring tissue integrity in a short time and reducing infections in the wound area, were obtained and extract-loaded liposome structures were formed for controlled release into the wound area. The wound healing efficacy of the composite material developed by loading herbal liposomes into levan-based hydrogel structures was evaluated in an in vitro wound model. Thus, a natural, biocompatible, biodegradable, and functional wound care product containing herbal liposomes suitable for both clinical applications and point-of-care use has been developed. The developed bioactive hydrogels are a promising therapeutic approach for the healing of acute wounds. Graphical abstract [Formula: see text]
{"title":"Levan-based bioactive hydrogels containing herbal liposome in treating acute wounds","authors":"Özlem Erdal Altıntaş, Pınar Aytar Çelik","doi":"10.1177/08839115231198528","DOIUrl":"https://doi.org/10.1177/08839115231198528","url":null,"abstract":"Hydrogels in active wound care products are 3D polymeric scaffolds that provide the desired moisture balance in the wound area with their high water retention capacity. Thanks to their biocompatible properties, flexible and porous structures, and high hydrophilic properties, they have frequently been preferred in the repair and regeneration of cells and tissues and the controlled release of bioactive substances to the target area. In this study, hydrogel structures were developed for the first time with levan produced by Halomonas elongata 153B halophilic bacteria. Extracts from Plantago lanceolata L., commonly known as a wound herb, which has medicinal importance for cell regeneration, ensuring tissue integrity in a short time and reducing infections in the wound area, were obtained and extract-loaded liposome structures were formed for controlled release into the wound area. The wound healing efficacy of the composite material developed by loading herbal liposomes into levan-based hydrogel structures was evaluated in an in vitro wound model. Thus, a natural, biocompatible, biodegradable, and functional wound care product containing herbal liposomes suitable for both clinical applications and point-of-care use has been developed. The developed bioactive hydrogels are a promising therapeutic approach for the healing of acute wounds. Graphical abstract [Formula: see text]","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.1177/08839115231198529
Mohadese Gharibblouk, Maryam Haji Ghasem Kashani, Meysam Nasiri, Arian Ehterami, Tayebeh Sadat Tabatabai, Majid Salehi
Bone grafts are widely used to improve bone healing but this method is costly and make some serious problems like infection in long-term use. To solve these problems, tissue engineering by using scaffolds made of different materials can be used as a supportive structure to enhance bone healing. In the current study, 3-D chitosan (CS) bone scaffold was developed by freeze-drying techniques for bone tissue engineering. The efficiency of the CS scaffold was improved by loading different concentrations of Hesperidin (Hes). Scaffolds were characterized by different mechanical and biological tests to evaluate their properties. In addition, the effect of scaffolds on bone healing was evaluated by a rat femur defect model. Results showed that the porosity of scaffolds was about 45–257 µm and Hes has a negative effect on the mechanical strength of scaffolds. Also, due to the hydrophilic properties of Hes, the degradation rate increased. Histological and CT-Scan evaluation showed that the treated groups which scaffold loaded with 1% and 10% of Hes were fully replaced by new bone and collagenous matrix compared to control and Hesperidin (0%, 0.01%, 0.1%) treated groups. The Runx2 gene expression was significantly increased by 1% and 10% compared to other groups. These results showed the positive effect of the fabricated scaffold on osteogenesis and bone healing and the possibility of using it in clinical trials.
{"title":"Evaluation of the therapeutic effect of chitosan scaffolds loaded with the different concentrations of Hesperidin in bone regeneration in rat model","authors":"Mohadese Gharibblouk, Maryam Haji Ghasem Kashani, Meysam Nasiri, Arian Ehterami, Tayebeh Sadat Tabatabai, Majid Salehi","doi":"10.1177/08839115231198529","DOIUrl":"https://doi.org/10.1177/08839115231198529","url":null,"abstract":"Bone grafts are widely used to improve bone healing but this method is costly and make some serious problems like infection in long-term use. To solve these problems, tissue engineering by using scaffolds made of different materials can be used as a supportive structure to enhance bone healing. In the current study, 3-D chitosan (CS) bone scaffold was developed by freeze-drying techniques for bone tissue engineering. The efficiency of the CS scaffold was improved by loading different concentrations of Hesperidin (Hes). Scaffolds were characterized by different mechanical and biological tests to evaluate their properties. In addition, the effect of scaffolds on bone healing was evaluated by a rat femur defect model. Results showed that the porosity of scaffolds was about 45–257 µm and Hes has a negative effect on the mechanical strength of scaffolds. Also, due to the hydrophilic properties of Hes, the degradation rate increased. Histological and CT-Scan evaluation showed that the treated groups which scaffold loaded with 1% and 10% of Hes were fully replaced by new bone and collagenous matrix compared to control and Hesperidin (0%, 0.01%, 0.1%) treated groups. The Runx2 gene expression was significantly increased by 1% and 10% compared to other groups. These results showed the positive effect of the fabricated scaffold on osteogenesis and bone healing and the possibility of using it in clinical trials.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135347169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.1177/08839115231195791
Abigail F Wu, Audrey L. Wu, T. Yeh, Pei-Chun Wong, Jia-Lin Wu
To enhance osteointegration between bone implants and bone tissues in implantation surgery, this study aimed to develop a hydrogel using chitosan and dextran to improve the cell proliferative ability, cell migratory capacity, and cell osteogenic ability. Herein, we developed two different hydrogel compositions with three different amounts of magnesium sulfate to produce six hydrogels for application as a filler for total knee replacement surgery to replace bone cement. The swelling property, degradation behavior, composition, and structure of the hydrogel were systemically investigated. Moreover, an MTT assay, scratch test, and alizarin red S staining were conducted to analyze the cell viability, migratory ability, and osteogenic response after being stimulated by the hydrogel. MC3T3-E1 preosteoblasts exhibited significantly improved cell proliferation, migratory capacity, osteogenesis, and mineralization due to MgSO4 in the hydrogel. Our work provides insights into the development of biomimetic and osteogenic hydrogels for bone integration in implantation surgery.
{"title":"Chitosan and dextran hydrogel as a filler for possible application in orthopedic implantation surgery","authors":"Abigail F Wu, Audrey L. Wu, T. Yeh, Pei-Chun Wong, Jia-Lin Wu","doi":"10.1177/08839115231195791","DOIUrl":"https://doi.org/10.1177/08839115231195791","url":null,"abstract":"To enhance osteointegration between bone implants and bone tissues in implantation surgery, this study aimed to develop a hydrogel using chitosan and dextran to improve the cell proliferative ability, cell migratory capacity, and cell osteogenic ability. Herein, we developed two different hydrogel compositions with three different amounts of magnesium sulfate to produce six hydrogels for application as a filler for total knee replacement surgery to replace bone cement. The swelling property, degradation behavior, composition, and structure of the hydrogel were systemically investigated. Moreover, an MTT assay, scratch test, and alizarin red S staining were conducted to analyze the cell viability, migratory ability, and osteogenic response after being stimulated by the hydrogel. MC3T3-E1 preosteoblasts exhibited significantly improved cell proliferation, migratory capacity, osteogenesis, and mineralization due to MgSO4 in the hydrogel. Our work provides insights into the development of biomimetic and osteogenic hydrogels for bone integration in implantation surgery.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":"17 1","pages":"358 - 367"},"PeriodicalIF":1.7,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82348868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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