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Evaluation of Total Concentrations and Extractable Fractionations of Cd, Co and Ni in Soils from Dumpsites across Rivers State, Nigeria 尼日利亚河流州垃圾场土壤中镉、钴和镍的总浓度和可萃取分馏评估
Pub Date : 2024-01-29 DOI: 10.4314/jasem.v28i1.1
R. N. Oduah, P. Amaibi, J. L. Konne, N. Boisa
The mobility of trace metals in soils strongly depends on the forms in which the metals are bound to major soil components. This study aims to determine the total concentrations and extractable fractionations of Cd, Co and Ni in soil samples collected from dumpsites across Rivers State, Nigeria. Solar Thermo Elemental Atomic Absorption Spectrometer model (SG 71906) was used after mixed acid digestion (HCl: HNO3 in a ratio of 3:1 v/v) and modified BCR sequential extraction procedure. The concentration levels of Cd, Co and Ni in all the samples varied, with mean values of 13.48 11.85, 25.29 17.62 and 20.52 15.66 mg/kg, respectively. Using the Community Bureau of Reference (BCR) sequential extraction procedure, the elements recoveries were within the acceptable range varying between 92.10% and 98.33% for Co and Cd, respectively. Data from the BCR extraction procedure revealed that the majority of Cd fraction was associated with residual fraction, Co fraction bound to the exchangeable fraction, while Ni was found to be associated with oxidisable fraction. These results suggest that the trace elements in the soil were highly mobile and bioavailable for plant uptake. Results from the findings particularly correlation analysis is indicative of the fact that some of the contaminants may have anthropogenic and natural origin. Hence, these contaminants could pose significant threat to human health and the environment.
痕量金属在土壤中的流动性在很大程度上取决于金属与主要土壤成分的结合形式。本研究旨在确定从尼日利亚河流州垃圾场采集的土壤样本中镉、钴和镍的总浓度和可萃取分馏度。在混合酸消化(HCl:HNO3,比例为 3:1 v/v)和改进的 BCR 顺序萃取程序之后,使用了太阳能热元素原子吸收光谱仪(SG 71906)。所有样品中的镉、钴和镍的浓度水平各不相同,平均值分别为 13.48 11.85、25.29 17.62 和 20.52 15.66 毫克/千克。采用社区参照局(BCR)的顺序萃取程序,钴和镉的回收率在可接受范围内,分别为 92.10% 和 98.33%。BCR 萃取程序的数据显示,大部分镉与残留物部分有关,钴与可交换部分有关,而镍与可氧化部分有关。这些结果表明,土壤中的微量元素具有很高的流动性和生物可利用性,可被植物吸收。研究结果特别是相关性分析表明,一些污染物可能来自人为和自然。因此,这些污染物可能对人类健康和环境构成重大威胁。
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引用次数: 0
Energy Sustainability: Bridging the Gap between Oil and Gas Operations and Community Well-Being in the Niger Delta Region, Nigeria 能源可持续性:缩小尼日利亚尼日尔三角洲地区石油和天然气业务与社区福祉之间的差距
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.20
J. Chijioke-Churuba
Sustainability is an important issue in energy provision for meeting increasing needs. However, harvesting energy resources in communities while protecting the ecosystem and reducing issues of environmental degradation to the barest minimum are the ideal target for stakeholders, but usually not always attainable. The objective of this paper is therefore to review the sustainable energy arrangements with ambitions to significantly bridge the gaps between oil and gas operations of firms and improved community well-being in the Niger Delta Region, Nigeria using secondary information and data harvested from previously published studies found Online. Specifically, the paper will examine some effects of oil and gas exploration in communities, examines metrics that can be used to track community well-being, the possible conflicts of interest that could occur between firms and host communities in oil and gas operations as well as measures in the past to ameliorate these challenges and improve the community well-being in issues pertaining to oil and gas operations of firms. The study recommends that continuous stakeholder engagement be carried out in order to make for smooth oil and gas operations. Furthermore, it makes for proffers workable solutions to the challenges that accentuate possible conflicts. The study also identifies different key stakeholders in this gap-bridging process and their various roles.
可持续性是满足日益增长的能源需求的一个重要问题。然而,在社区收获能源资源的同时保护生态系统并将环境退化问题减少到最低限度是利益相关者的理想目标,但通常并不总是能够实现。因此,本文的目的是利用从网上以前发表的研究报告中收集的二手信息和数据,对可持续能源安排进行审查,以期大大缩小尼日利亚尼日尔三角洲地区企业的石油和天然气业务与改善社区福祉之间的差距。具体而言,本文将研究石油和天然气勘探对社区的一些影响,探讨可用于跟踪社区福祉的衡量标准、企业与所在社区在石油和天然气运营中可能出现的利益冲突,以及过去为改善这些挑战和提高社区福祉而采取的与企业石油和天然气运营相关的措施。研究建议,为了使石油和天然气运营顺利进行,应持续开展利益相关者参与活动。此外,研究还针对可能加剧冲突的挑战提出了可行的解决方案。本研究还确定了这一弥补差距过程中的不同主要利益相关者及其各种作用。
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引用次数: 0
Evaluation of Essential Metal Concentration and Possible Potential Health Risk in Chicken Giblets Commonly Sold to Consumers in Lokoja Main Market, Kogi State, Nigeria 评估尼日利亚科吉州洛科贾主要市场上常见的鸡内脏中的基本金属浓度和可能的潜在健康风险
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.27
Emurotu, JE Habib
The objective of this work was to evaluate the concentrations of essential metals (K, Na, Ca, Mg, Fe, Cu, Se, B, P, and Zn) and potential health risks in chicken giblets commonly sold to consumers in Lokoja's main market, Kogi State, Nigeria. Using appropriate standard methods, samples were digested with 65% HNO3, and the digest was analyzed using an Inductively Coupled Plasma-Optical Emission Spectrometer. The mean concentrations (mg/kg) in gizzard ranged from 0.06±0.08 (Cu) to 28.1±6.9 (P), kidney 0.04±0.01 (Cu) to 24±10 (P), and liver 0.09±0.10 (Cu) to 47±11(P). All the essential metals examined were below the FAO and WHO's permissible standards. The observed trend in the accumulation of metal concentration in chicken giblets was P > Na > Ca > Fe > Mg > Zn > B > Se > K, Cu. The health risk analyses revealed that the current exposure levels will not have a major negative impact on human health, as the hazard quotient for each metal was less than one (<1) and the overall hazard index was also less than 1. Although the population will not be exposed to the potential health risks from these metals presently, there is a need to regularly assess the levels of heavy metals in chicken giblets to maintain protection against negative health effects.
这项工作的目的是评估尼日利亚科吉州洛科贾主要市场上通常出售给消费者的鸡内脏中必需金属(K、Na、Ca、Mg、Fe、Cu、Se、B、P 和 Zn)的浓度以及潜在的健康风险。采用适当的标准方法,用 65% 的 HNO3 对样品进行消化,然后用电感耦合等离子体-光发射光谱仪对消化液进行分析。胗中的平均浓度(毫克/千克)为 0.06±0.08(铜)至 28.1±6.9(磷),肾中的平均浓度(毫克/千克)为 0.04±0.01(铜)至 24±10(磷),肝中的平均浓度(毫克/千克)为 0.09±0.10(铜)至 47±11(磷)。所有检测的基本金属含量均低于粮农组织和世卫组织的允许标准。鸡内脏中金属浓度的累积趋势为 P > Na > Ca > Fe > Mg > Zn > B > Se > K、Cu。健康风险分析表明,目前的暴露水平不会对人类健康产生重大负面影响,因为每种金属的危害商数均小于 1(<1),总体危害指数也小于 1。虽然目前人们不会暴露于这些金属的潜在健康风险中,但有必要定期评估鸡内脏中的重金属水平,以保持对健康的负面影响。
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引用次数: 0
Effects of Fly Ash on Compressive Strength and Durability Properties of Lean Concrete 粉煤灰对轻质混凝土抗压强度和耐久性能的影响
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.32
J. O. Ohwofasa, C. Ikumapayi, C. Arum
Concrete production and waste generation are among human activities that negatively impact the environment. This study investigated the effects of fly ash on compressive strength and durability properties of lean concrete using appropriate standard methods. The results showed that the compressive strength of the control mix is higher than those of fly ash concrete mixes. The compressive strength of the fly ash blended mixes decreased as the replacement level increased. However, the strength increased with hydration time, and the optimal replacement level being 5%. While the chloride ion penetration and water absorption rate decreased, the sulfate resistance increased with hydration time and replacement level. The fly ash mixes exhibited better durability properties than the control mix in all durability tests conducted. The SEM-EDX analysis showed that mixes with well-packed microstructures exhibited favorable compressive and durability properties. In conclusion, Class C fly ash is recommended for lean concrete mixes at a replacement level of up to 15% when compressive strength is the primary concern, while mixes with a replacement level of 25% or more are recommended in areas susceptible to sulfate attack.
混凝土生产和废物产生是对环境产生负面影响的人类活动之一。本研究采用适当的标准方法研究了粉煤灰对瘦身混凝土抗压强度和耐久性能的影响。结果表明,对照混合料的抗压强度高于粉煤灰混凝土混合料。粉煤灰掺合料的抗压强度随着掺量的增加而降低。然而,强度随着水化时间的延长而增加,最佳掺量为 5%。虽然氯离子渗透率和吸水率有所下降,但抗硫酸盐性能却随着水化时间和掺量的增加而提高。在所有耐久性测试中,粉煤灰混合料的耐久性能都优于对照混合料。SEM-EDX 分析表明,具有良好堆积微结构的混合料具有良好的抗压和耐久性能。总之,当抗压强度是主要考虑因素时,建议将 C 级粉煤灰用于掺量不超过 15%的瘦身混凝土拌合物,而在易受硫酸盐侵蚀的地区,建议将掺量控制在 25%或以上。
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引用次数: 0
Oral Vitamin C Intake Ameliorates Crude Oil-Polluted Water-Induced Jejunal Contractile Dysfunctions in Wistar rats 口服维生素 C 可改善原油污染水诱发的 Wistar 大鼠空肠收缩功能障碍
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.26
S. A. Salami, A. J. Oyemakinde, M. O. Allen, H. Salahdeen, I. I. Olatunji-Bello, B. Murtala
Inhabitants of rural Niger-Delta oil communities in Nigeria often, unintentionally, consume crude oil polluted water (COCW) due to crude oil spills in the region. The impact of vitamin C supplementation during COCW ingestion on the contractile mechanism of the jejunum is not fully known. Hence, this study investigates the outcomes of COCW water and vitamin C intake on the smooth muscle activity of the jejunum in Wistar rats using standard techniques. Data obtained showed that jejunal tissue SOD concentration was significantly reduced, while jejunal tissue MDA concentration was significantly increased in the COCW-only treated group. Contraction mediated by acetylcholine was significantly increased in the COCW-only treated rats. Calcium and potassium ion influx significantly increased jejunal contraction in the COCW-only rats. Incubation of the jejunal tissue in nifedipine, indomethacin, and L-NAME significantly increased acetylcholine-mediated contractions in the jejunal tissue of the COCW-only treated rats when compared to other groups. COCW causes free radical-induced jejunal damage that results in impaired jejunal contractile activity mediated by the M2 muscarinic receptor, nitric oxide synthase activity, voltage-gated large-conductance calcium channels, potassium channels, and prostaglandins. However, the oral intake of Vitamin C supplementation significantly ameliorated impairments by enhancing jejunal antioxidant activity.
尼日利亚尼日尔河三角洲农村石油社区的居民经常无意中饮用因该地区原油泄漏而受到原油污染的水(COCW)。在摄入 COCW 期间补充维生素 C 对空肠收缩机制的影响尚不完全清楚。因此,本研究采用标准技术调查了摄入 COCW 水和维生素 C 对 Wistar 大鼠空肠平滑肌活动的影响。数据显示,仅摄入 COCW 的大鼠空肠组织 SOD 浓度明显降低,而空肠组织 MDA 浓度明显升高。纯 COCW 处理组大鼠由乙酰胆碱介导的收缩明显增加。钙离子和钾离子的流入明显增加了纯 COCW 大鼠的空肠收缩。与其他组相比,用硝苯地平、吲哚美辛和 L-NAME 培养空肠组织可明显增加只用 COCW 处理的大鼠空肠组织由乙酰胆碱介导的收缩。慢性盲肠炎会引起自由基诱导的空肠损伤,导致由 M2 肌卡因受体、一氧化氮合酶活性、电压门控大电导钙通道、钾通道和前列腺素介导的空肠收缩活性受损。然而,口服维生素 C 补充剂可增强空肠抗氧化活性,从而明显改善空肠损伤。
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引用次数: 0
Garden Egg (Solanum aethiopicum) as a Mystical plant in Akabo, South Eastern Nigeria: Health and Economic Implications 尼日利亚东南部阿卡博的神秘植物花园蛋(Solanum aethiopicum):对健康和经济的影响
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.38
E. Opara, G. A. Udourioh
Garden egg, popularly known as eggplant, is one of the strange and enigmatic vegetable plants that can energize and heal the body as well as the spirit. It is a manifestation of the wonders of God’s creation and magnanimity. As recorded in the holy books, God made man and woman and told them to eat vegetables to keep their bodies and spirits healthy and together. Garden egg is one fruit that is highly acceptable and valued when offered to visitors.  In Akabo, South eastern Nigeria, there is a practice called "Ofe", which translates to "garden eggplant mystics". It is a significant mystical practice that helps to maintain harmony among people and between bodies and spirits.  In this study, the mystical, medicinal and economic implications of garden egg as applied in Akabo community are presented. Interview data and library databases were used to analyze the sources of information by theme. The origin, classes, and chemical composition of garden eggs as it affects its medicinal efficacy have been highlighted. Insights to the cultural significance of garden egg in Africa are also documented. The overall benefits of the various parts of the eggplant, viz: fruits, leaves, stems and roots and their economic importance to the global society have been presented. This study will serve as a useful piece to the global community to unravel the untapped potentials of garden egg.
鸡蛋花,俗称茄子,是一种奇特而神秘的蔬菜植物,它能给人的身体和精神带来活力和疗效。它体现了上帝创造的奇迹和宽宏大量。正如圣书中记载的那样,上帝创造了男人和女人,并告诉他们要吃蔬菜来保持身体和精神的健康与和谐。园蛋是一种非常容易被游客接受和喜爱的水果。 在尼日利亚东南部的阿卡博,有一种叫做 "Ofe "的习俗,翻译过来就是 "神秘的花园茄子"。这是一种重要的神秘习俗,有助于保持人与人之间以及身体与精神之间的和谐。 本研究介绍了在阿卡博社区应用的园茄的神秘、药用和经济意义。访谈数据和图书馆数据库用于按主题分析信息来源。重点介绍了园蛋的起源、种类和化学成分对其药用功效的影响。此外,还记录了花园蛋在非洲的文化意义。此外,还介绍了茄子各部分(即果实、叶、茎和根)的整体效益及其对全球社会的经济重要性。这项研究将成为全球社会发掘园茄尚未开发的潜力的有用资料。
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引用次数: 0
Biological Aspects of Preferred Fish Species in White Volta at Pwalugu, Ghana: A pre-impoundment Study 加纳 Pwalugu 的白沃尔特海峡首选鱼种的生物学方面:蓄水前研究
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.10
D. N. Akongyuure, M. A. Adakpeya, E. H. Alhassan
The socio-economic benefits derived from impounding rivers are extremely important but the ecological imbalances that are usually created in the process can be devastating. It is therefore imperative to understand some aspects of the biology of fishes before the impoundment of a river. This study was conducted to assess the length-weight relationship, condition factor, feeding, and reproductive ecology of fish species with high local market value. Biometric measurements were obtained from monthly samples of fish at two sites. Specimens were transported to the laboratory for gut and gonad analyses. Auchenoglanis occidentalis, Marcussenius senegalensis and Heterobranchus bidorsalis were the most preferred fish species. All three fish species recorded ‘b’ values less than 3 which implies they exhibited a negative allometric growth. Their condition factors (K) indicated they were in good health since K values were greater than 1. A. occidentalis and H. bidorsalis demonstrated a feeding habit comparable to that of an omnivore, whilst M. senegalensis presented a feeding habit that implied it was an invertivore. M. senegalensis registered the uppermost mean gonado-somatic index (GSI) (2.14 ± 0.83) whereas the highest mean fecundity (495 ± 450.96) was noted in A. occidentalis. This study contributed bio-ecological baseline information about the ichthyofauna prior to the damming of the reservoir in the Pwalugu area of the White Volta.
拦河蓄水带来的社会经济效益极其重要,但在此过程中通常会造成生态失衡,具有破坏性。因此,在河流蓄水之前了解鱼类生物学的某些方面势在必行。本研究旨在评估当地市场价值较高的鱼类物种的体长-体重关系、状态因子、摄食和繁殖生态。每月在两个地点采集鱼类样本,进行生物计量测量。标本被运送到实验室进行肠道和性腺分析。Auchenoglanis occidentalis、Marcussenius senegalensis 和 Heterobranchus bidorsalis 是最受欢迎的鱼种。这三种鱼的 "b "值均小于 3,这意味着它们呈现负异速生长。它们的条件因子(K)表明它们的健康状况良好,因为 K 值大于 1。A occidentalis 和 H. bidorsalis 的摄食习性类似于杂食动物,而 M. senegalensis 的摄食习性则表明它是一种无脊椎动物。M.senegalensis的平均性腺指数(GSI)最高(2.14 ± 0.83),而A. occidentalis的平均繁殖力最高(495 ± 450.96)。这项研究为白沃尔特省普瓦卢古地区水库筑坝前的鱼类提供了生物生态基线信息。
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引用次数: 0
Assessment of Growth Characteristics, Diversity and Structure of Tree Species in Opara Forest Reserve, Oyo State, Southwest Nigeria 尼日利亚西南部奥约州奥帕拉森林保护区树种的生长特征、多样性和结构评估
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.31
O. A. Ogidan, A. F. Aderounmu, A. A. Ekaun, O. A. Ogundana, R. T. Afolabi
Having information about the existing stands and their structure within a forest is important, as it helps in understanding the biodiversity depth and climate change adaptation potential. Therefore, the objective of this study was to assess the growth characteristics, diversity and structure of tree species in the Opara Forest Reserve on the Nigeria-Benin Republic Fringes in Oyo State, Southwest Nigeria using appropriate standard techniques. A total of 218 trees were enumerated and assessed.  The results indicated that the forest reserve is primarily characterized by the prevalence of savanna species. The dominant species within the study area consists of 80 stands of Anogeissus leiocarpus; Vitellaria paradoxa exhibits the highest mean diameter at breast height (dbh), measuring 100.09 cm and Acacia faidherbia had highest value in height. The forest reserve is characterised by the dominance of trees within the 6-10 metre height range. In conclusion, the forest reserve exhibits a moderate level of species variety with favourable growth characteristics and the average height of the trees are typical of a savanna forest.
掌握森林中现有林分及其结构的信息非常重要,因为这有助于了解生物多样性的深度和适应气候变化的潜力。因此,本研究的目的是采用适当的标准技术,评估尼日利亚西南部奥约州尼日利亚-贝宁共和国边缘地带奥帕拉森林保护区内树木的生长特征、多样性和结构。共对 218 棵树木进行了计数和评估。 结果表明,该森林保护区的主要特征是热带稀树草原物种盛行。研究区内的主要树种包括 80 株雷公藤;藤黄属植物的平均胸径(dbh)最高,达 100.09 厘米,金合欢属植物的高度值最高。该森林保护区的特点是以 6-10 米高度范围内的树木为主。总之,该森林保护区的物种多样性处于中等水平,具有良好的生长特性,树木的平均高度是典型的热带稀树草原森林。
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引用次数: 0
Multivariate Statistical Analysis for Assessment of Groundwater Quality around Dumpsites in Bauchi metropolis, Bauchi State, Nigeria 尼日利亚包奇州包奇市垃圾场周围地下水质量评估的多元统计分析
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.21
A. F. Tijjani, B. Alhassan, U. Salisu, A. M. Hassan
Groundwater is an important source of drinking water and the protection of groundwater is a major environmental issue as the importance of water quality to human health. Hence, the objective of this study was to assess the groundwater quality around dumpsites in Bauchi metropolis, Bauchi State, Nigeria using standard analytical techniques and multivariate statistical analysis methods such as correlation analysis (CA), principal component analysis (PCA), and hierarchical cluster analysis (HCA).  The mean concentration of the parameters in ground water samples ranged from pH 7.55 to 9.06, temperature 32.55 to 34.25 oC, conductivity 54.80 to 65.38 μS/cm, salinity 0.20 to 0.29 mg/L, TSS 0.36 to 1.93 mg/L, TDS 57.53 to 59.95 mg/L, HCO3 26.80 to 31.00 mg/L, PO43- 1.05 to 2.06 mg/L, SO42- 2.25 to 3.26 mg/L, NO3- 1.40 to 4.50 mg/L, NO2- 0.01 to 0.03 mg/L, and Cl- 2.14 to 8.31 mg/L. The result shows that the investigated parameters were below the WHO, permissible limit, except PO43-. The concentration of the parameters were in following trend; TDS > conductivity > temperature > HCO3> pH > NO3> SO4>Cl> PO4> TSS > salinity > NO2. Multivariate analysis revealed that the main sources of water quality parameters might be related to dumpsites around the study area. According to the HCA, and PCA, the parameters were divided into three groups; the first group correlated with Salinity, TSS, SO4, NO3 and NO2; the second group correlated with TDS and HCO3, and the third with pH, temperature, and conductivity. The hydrochemistry of groundwater from the area reveals that the water could be applied to irrigation.
地下水是重要的饮用水源,由于水质对人类健康的重要性,保护地下水是一个重要的环境问题。因此,本研究的目的是采用标准分析技术和多元统计分析方法,如相关分析 (CA)、主成分分析 (PCA) 和层次聚类分析 (HCA),评估尼日利亚包奇州包奇市垃圾场周围的地下水质量。 地下水样本中各参数的平均浓度范围为:pH 值 7.55 至 9.06,温度 32.55 至 34.25 oC,电导率 54.80 至 65.38 μS/cm,盐度 0.20 至 0.29 mg/L,总悬浮固体 0.36 至 1.93 毫克/升,TDS 57.53 至 59.95 毫克/升,HCO3 26.80 至 31.00 毫克/升,PO43- 1.05 至 2.06 毫克/升,SO42- 2.25 至 3.26 毫克/升,NO3- 1.40 至 4.50 毫克/升,NO2- 0.01 至 0.03 毫克/升,Cl- 2.14 至 8.31 毫克/升。结果表明,除 PO43- 外,其他调查参数均低于世界卫生组织的允许限值。各参数的浓度变化趋势如下:TDS > 电导率 > 温度 > HCO3> pH > NO3> SO4>Cl> PO4> TSS > 盐度 > NO2。多变量分析表明,水质参数的主要来源可能与研究区域周围的垃圾场有关。根据 HCA 和 PCA,参数被分为三组,第一组与盐度、TSS、SO4、NO3 和 NO2 相关,第二组与 TDS 和 HCO3 相关,第三组与 pH、温度和电导率相关。该地区地下水的水化学特征表明,这些水可用于灌溉。
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引用次数: 0
Bioactive And Cytotoxic Potentials of the Extract Fractions of Strophanthus Hispidus on Neuroblastoma (SH-SY5Y) Cell Line Model of Alzheimer’s Disease 石蒜提取物对阿尔茨海默病神经母细胞瘤(SH-SY5Y)细胞系模型的生物活性和细胞毒性潜力
Pub Date : 2023-11-28 DOI: 10.4314/jasem.v27i11.36
P. A. Akinyemi, O. A. Adebesin, O. M. Arotiba, A. Adewole, J. E. Okpuzor
Alzheimer disease (AD) is a neurodegenerative disorder that affect memory and cognitive function. Currently, the available agents used in managing this condition are associated with severe side effects and have limited efficacy in reducing or modulating events associated with AD. Hence, there is a need for an alternative therapy with fewer side effects and better efficacy. Strophantus hispidus (SH) is a plant product widely used in Africa for its therapeutic potential.  This study was conducted to assess the bioactive constituent of the SH extract fractions, as well as the cytotoxic potential of these fractions.  The root of SH was pulverized and extracted with 80% methanol. The crude extract was then fractionated with Chloroform, Hexane, and Ethylacetate. The extract and fractions were then subjected to Gas Chromatography-Mass Spectrometry (GC-MS).  SH-SY5Y cell line model of AD was treated with graded concentrations (9.77 – 5000 µg/mL) of the SH extract and fractions for 24 hours. Cell viability assay was performed using WST-8 proliferation assay. The EC50 was determined to be 48.4 μg/mL, 15.16 μg/mL, and 151 μg/mL for cells treated with Chloroform, Ethylacetate, and Hexane fractions of Strophantus hispidus respectively. The selectivity index (SI) greater than 3 of the hexane and ethylacetate fractions were determined to be safe, less toxic, and effective in improving cellular growth.
阿尔茨海默病(AD)是一种影响记忆和认知功能的神经退行性疾病。目前,用于治疗这种疾病的药物都有严重的副作用,而且在减少或调节与阿尔茨海默病相关的事件方面疗效有限。因此,需要一种副作用小、疗效好的替代疗法。Strophantus hispidus(SH)是一种在非洲广泛使用的植物产品,具有治疗潜力。 本研究旨在评估糙叶芹提取物馏分的生物活性成分以及这些馏分的细胞毒性潜力。 将 SH 的根部粉碎并用 80% 的甲醇提取。然后用氯仿、正己烷和乙酸乙酯对粗提取物进行分馏。然后对提取物和馏分进行气相色谱-质谱分析(GC-MS)。 用不同浓度(9.77 - 5000 µg/mL)的 SH 提取物和馏分处理 AD 的 SH-SY5Y 细胞株模型 24 小时。使用 WST-8 增殖试验进行细胞活力检测。氯仿、乙酸乙酯和正己烷馏分处理细胞的 EC50 分别为 48.4 μg/mL、15.16 μg/mL 和 151 μg/mL。经测定,正己烷和乙酸乙酯馏分的选择性指数(SI)大于 3,因此安全、毒性低,并能有效改善细胞生长。
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引用次数: 0
期刊
Journal of Applied Sciences and Environmental Management
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