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Correlation of Density and Water Quality to Cortisol of Cantang Grouper Fish (Epinephelus fuscoguttatus X Epinephelus lanceolatus) in Floating Net Cages 坎塘石斑鱼浮网网箱密度和水质与皮质醇的关系
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.36661
L. Sulmartiwi, Bintang Eka Nugraha Satia Aditama, Isnatul Umu Shohifah, Alfian Rahmadhani Satria Hani Saputra
Cantang grouper is a hybrid resulting from crosses between female tiger grouper (E. fuscoguttatus) and male kertang grouper (E. lanceolatus). Cultivation with floating net cages is one of the attempts made to cultivate cantang grouper fish. Problems that arise are the non-uniformity of the size of the fish when harvesting, this problem can lead to a decrease in selling prices and maintenance that tends to be longer. Metabolic disorders in living beings can be caused due to physiological stress and one of the causes can arise from water quality. Water quality is closely related to feeding and stocking density because the increase in feed and stocking density can affect the accumulation of feces to stress which causes slow growth. This study was conducted to find out the influence of density and water quality factors that play the most important role in the stress of cantang grouper fish based on indicators of cortisol levels. Cortisol testing is performed using the elisa method with Bioassay Technology Laboratory E0014Fi kit type. Data are processed using multiple linear regression test analysis that serves to determine the relationship and influence of the free variable (independent) X on non-free variables (dependent) Y. Regression analysis is used to determine the relationship between factors that are causal relationships. After the regression test, then the Analysis of Variance (ANNOVA) test is conducted, to find out the difference and influence of dependent parameters with significantly independent. There is a strong influence and correlation between water quality factors simultaneously to the cortisol levels of cantang grouper fish in floating nets, as can be seen from the R value of 0.546. The most influential water quality factor was nitrite with an effect of 19.7%. The next most significant water quality factor was ammonia with an effect of 37.1%. The indicated cortisol value can be known that fish are not in a state of stress due to increased NO2 and NH3. Density has no significant effect on cortisol because of its significance value above (p>0.05).
坎塘石斑鱼是由雌性虎石斑鱼(E.fuscoguttatus)和雄性柯塘石斑鱼类(E.lanceolatus)杂交而成的杂交种。浮网网箱养殖是康塘石斑鱼养殖的尝试之一。出现的问题是捕捞时鱼的大小不一致,这个问题可能导致售价下降,维护时间往往更长。生物代谢紊乱可能是由生理压力引起的,其中一个原因可能是水质。水质与饲养和放养密度密切相关,因为饲养密度和放养密度的增加会影响粪便的积累,从而导致生长缓慢。本研究旨在根据皮质醇水平指标,找出在康塘石斑鱼应激中起最重要作用的密度和水质因素的影响。皮质醇测试采用elisa方法,生物测定技术实验室E0014Fi试剂盒类型。使用多元线性回归测试分析来处理数据,该分析用于确定自由变量(自变量)X对非自由变量(因变量)Y的关系和影响。回归分析用于确定因果关系因素之间的关系。回归检验后,进行方差分析(ANNOVA)检验,找出具有显著独立性的相关参数的差异和影响。从0.546的R值可以看出,水质因素同时对浮网中康塘石斑鱼的皮质醇水平有很强的影响和相关性。影响最大的水质因素是亚硝酸盐,影响19.7%。其次是氨,影响37.1%。所显示的皮质醇值可以看出,由于NO2和NH3的增加,鱼类没有处于应激状态。密度对皮质醇没有显著影响,因为其显著性值高于(p>0.05)。
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引用次数: 0
Characterization of Salt Quality from Artemia (Artemia franciscana) Cultured Water with Dunaliella salina as Natural Feed 盐藻作为天然饲料对卤虫(Artemia francicana)培养水盐品质的表征
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.34790
H. S. Islamiyah, Mochammad Amin Alamsjah, Laksmi Sulmartiwi
The high demand for domestic salt cannot be achieved by local salt production from traditional salt and PT. Garam fluctuates every year. In addition, the quality of traditional salt is still low. This causes the need for domestic salt consumption to be met by imported salt. Therefore, it is necessary to have an alternative to meet the domestic salt demand, namely the revitalization of salt ponds for the development of mass Artemia cultivation that produces both Artemia production and biomass and produces salt in one location. In its natural habitat, Artemia uses microalgae as its main food source. The quality of microalgae feeds greatly determines the production of Artemia franciscana. The availability of nutrients is a factor that determines the rate of growth, so the amount and quality of feed are the main factors to meet the nutritional content for optimal development. Therefore, the salt production in this study used saltwater cultured Artemia with Dunaliella salina as a natural feed with a water age of 3rd,7th, and 14th days. This study aimed to determine the best age of Artemia water cultured with Dunaliella salina as a natural feed in salt production. The results showed that the different ages in Artemia water cultured with Dunaliella salina had a significant effect (p < 0.05) on salt characteristics. The best result was D7 which is salt from Artemia cultured water with Dunaliella salina as a natural feed with water age 7th day. Their respective values are 0.66a + 0.33 water content, 91.55 + 0.32 NaCl, 0.87 water-insoluble part, 0.39% + 0.01 Ca, and 1.25a + 0.05 Mg.
国内对盐的高需求无法通过当地传统盐和PT生产来实现。Garam每年都在波动。此外,传统盐的质量仍然很低。这导致国内食盐消费的需要由进口食盐来满足。因此,有必要有一个替代方案来满足国内对盐的需求,即振兴盐池,发展大规模的蒿种植,既生产蒿,又生产生物质,在一个地方生产盐。在其自然栖息地,Artemia以微藻为主要食物来源。微藻饲料的质量在很大程度上决定了苜蓿的产量。营养物质的可利用性是决定生长速度的一个因素,因此饲料的数量和质量是满足最佳发育营养含量的主要因素。因此,本试验采用含盐杜氏藻的盐水养殖Artemia作为天然饲料,水龄分别为3、7、14 d。本研究旨在确定含盐杜氏藻的水中养殖青蒿作为天然饲料用于制盐的最佳年龄。结果表明,不同龄期的盐杜氏藻对水中青蒿的盐分特性有显著影响(p < 0.05)。产盐效果最好的是D7,即以产盐杜氏藻为天然饲料,水龄为7 d的Artemia养殖水中的盐。分别为0.66a + 0.33含水量、91.55 + 0.32 NaCl、0.87水不溶性成分、0.39% + 0.01 Ca、1.25a + 0.05 Mg。
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引用次数: 0
Solid Fermentation of Pelletized Fish Feeds Containing Black Soldier Fly (Hermetia illucens) Larvae Meal to Enhance Growth Performance of Catfish (Clarias sp.) 黑兵蝇幼虫粉颗粒鱼饲料固体发酵提高鲇鱼生长性能的研究
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.34956
Cita Mahardika Hariyono, Catur Sriherwanto, H. Harijono
Unsustainable use of fish meal as protein and lipid ingredients in aquafeed has driven the search for alternative. Black soldier fly (BSF) (Hermetia illucens) larvae have been widely studied for the substitution purpose. This study aims at investigating the effect of solid fermentation of pelleted diets containing BSF larvae meal (BSFLM) on the growth performance of catfish Clarias sp. Four feed pellets were formulated: F1 (non-fermented, 60% BSFLM), F2 (fermented, 60% BSFLM), F3 (non-fermented, 50% BSFLM), and F4 (fermented, 50% BSFLM). These formulated diets and commercial feed pellets (positive control) were separately fed to the catfishes in 5 aquaria (10 fishes per aquaria in triplicate). Results showed that the fermentation increased the protein level by 10–11%, but kept the crude lipid concentrations unchanged. Specific growth rate (SGR) and weight gain (WG) profiles for fermented feeds F2 and F4 were higher than the non-fermented feeds F1 and F3, respectively. F4  gave the best feed conversion ratio (FCR) of 1.78, which was 15–25% more efficient than F1, F2, and F3. Compared to the commercial feed, the 4 formulated feeds underperformed by around 50% in all of the growth parameters except survival rates (SR) which were not significantly different  (93–100%).
鱼粉在水产饲料中作为蛋白质和脂质成分的不可持续使用推动了对替代品的探索。黑兵蝇(BSF)幼虫已被广泛研究用于替代目的。本研究旨在研究含有BSF幼虫粉(BSFLM)的颗粒饲料的固体发酵对鲶鱼Clarias sp.生长性能的影响。配制了四种饲料颗粒:F1(非发酵,60%BSFLM)、F2(发酵,60%SFLM)、F3(非发酵的,50%BSFLM和F4(发酵,50%BSFL)。将这些配方饲料和商业饲料颗粒(阳性对照)分别喂给5个养殖场的鲶鱼(每个养殖场10条鱼,一式三份)。结果表明,发酵使蛋白质水平提高了10-11%,但保持了粗脂质浓度不变。发酵饲料F2和F4的比生长率(SGR)和增重(WG)曲线分别高于未发酵饲料F1和F3。F4的最佳饲料转化率(FCR)为1.78,比F1、F2和F3高15-25%。与商业饲料相比,除了存活率(SR)没有显著差异(93–100%)外,4种配方饲料在所有生长参数上都表现不佳约50%。
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引用次数: 0
Detection of Salmonella spp., Escherichia coli, and Listeria monocytogenes in Tuna by Multiplex PCR 多重PCR检测金枪鱼中沙门氏菌、大肠杆菌和单核增生李斯特菌
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.36509
Kristin Natalia, M. Wahjudi, Tjie Kok
Fishery products are types of commodity with various levels of development including the addition of food additives. They are very susceptible to patogenic microbial contamination hence they can cause food poisoning and cause disease outbreaks in consumers. In this study, tuna fish spiked with Salmonella spp., Escherichia coli, and Listeria monocytogenes bacteria is examined for the bacteria contamination. The examination of these patogenic bacteria is carried out using multiplex PCR (mPCR), a modern technique developed to detect target DNAs of bacteria simultaneously. The results of the examination are compared to biochemical test for sensitivity and time-efficiency. Based on the results obtained, mPCR method is ±1250-fold more sensitive and ±80-fold more time-efficient than the biochemical tests. Therefore, mPCR method could be recommended to be used as a tool for detection of bacteria in fishery products.
渔业产品是一种具有不同发展水平的商品,包括添加食品添加剂。它们非常容易受到致斑微生物污染,因此可能导致食物中毒,并在消费者中引发疾病爆发。在这项研究中,对掺有沙门氏菌、大肠杆菌和李斯特菌的金枪鱼进行了细菌污染检测。使用多重PCR(mPCR)对这些致斑细菌进行检测,这是一种同时检测细菌靶DNA的现代技术。将检查结果与生化测试的灵敏度和时间效率进行了比较。基于所获得的结果,mPCR方法比生化测试的灵敏度高±1250倍,时间效率高±80倍。因此,mPCR方法可作为检测水产中细菌的工具。
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引用次数: 0
Effect of Feeding Fermented Leucaena leucocephala Seed Meal on Growth and Protein Content of Osphronemus gouramy Juvenile 投喂发酵银合欢籽粕对食虾幼鱼生长和蛋白质含量的影响
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.25879
Putri Dista Ananda, S. Safrida, K. Khairil, H. Hasanuddin, Asiah M.D.
Gouramy (Osphronemus gouramy Lac.) is one of the original fish from Indonesia that lives in fresh water and has high economic value because of its dense texture, high nutrition, and delicious taste so that it is widely favored by Indonesians. The results of juvenile cultivation activities have not been going well so far, this is because the growth of gouramy’s juvenile is slower than the growth of other freshwater fish’s juvenile. One alternative that can increase the growth and protein content of gouramy juvenile is to utilize of the availability of lamtoro seeds. The purpose of this study was to determine the effect of feeding lamtoro seed fermentation on growth and protein content of gouramy’s juvenile. This study used experimental method with a completely randomized design (CRD with 6 treatments and 4 replications). Data were analyzed using Analysis of Variance Test (ANOVA) and further test in the form of Honest Significant Difference (Tukey HSD Test) at the 95% confidence level. The results showed that P3 with the addition of fermented lamtoro seed flour 40% can increase the growth and protein content of gouramy juvenile. The conclusion of this study was lamtoro seed fermented feed has effect in increasing growth and protein content of gouramy’s juvenile.
Gouramy (Osphronemus Gouramy Lac.)是印度尼西亚原产鱼类之一,生活在淡水中,因其质地致密、营养丰富、味道鲜美而具有很高的经济价值,受到印尼人的广泛青睐。到目前为止,幼鱼养殖活动的结果并不顺利,这是由于食用鱼幼鱼的生长速度比其他淡水鱼幼鱼的生长速度慢。一种可以提高食用鱼幼鱼的生长和蛋白质含量的方法是利用食用鱼的种子。本试验旨在研究投喂拉托罗种子发酵对食用鱼幼鱼生长和蛋白质含量的影响。本研究采用完全随机设计(CRD, 6个处理,4个重复)的实验方法。数据分析采用方差分析检验(ANOVA),进一步检验采用诚实显著差异检验(Tukey HSD检验),置信水平为95%。结果表明,在P3中添加40%发酵lamtoro籽粉,可提高食糜幼鱼的生长和蛋白质含量。本研究的结论是,拉托罗籽发酵饲料具有提高食糜幼鱼生长和蛋白质含量的作用。
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引用次数: 1
Embryonic and Larval Development Stages of the African Catfish Clarias gariepinus (Burchell, 1822) (Teleostei, Clariidae) in The Ouargla, Algeria 阿尔及利亚瓦尔格拉非洲鲇鱼Clarias gariepinus (Burchell, 1822) (Teleostei, Clariidae)的胚胎和幼虫发育阶段
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.31871
Ibrahim El Khalil Behmene, Benabdallah Bachir Bouiadjra, Miloud Attou, A. Homrani
This study was designed to investigate for the first time in the region of Ouargla, Algeria, the events and timing of the embryonic development of African catfish, C. gariepinus  (Burchell, 1822). The embryonic development was carefully monitored using a binocular magnifier 10 and 20 lenses that reveal details on live specimens from fertilization to the first take of food. The offensive and foul odor continues to be the characteristic smell of the hatching stage. Pigmentation and continuous spread of cephalo-caudal melanophore in fry hatched on the first day. The barely hatched larvae are photo-phobic. The yellow reserve is reduced and leads to the ability to swim easily, and the majority starts feeding on the fourth day. The vitellin reserve is significantly reduced, allowing the larvae to feed exogenously. The events observed in our opinion demonstrate the presence of two exceptionally critical parameters: the use of a high-quality diet and the guarantee of a clean and quality source of water, meeting the standards of aquaculture farming.
本研究旨在首次在阿尔及利亚的瓦格拉地区调查非洲鲶鱼C.gariepinus胚胎发育的事件和时间(Burchell,1822)。使用双目放大镜10和20个镜头仔细监测胚胎发育,这些镜头揭示了从受精到第一次进食的活标本的细节。难闻的气味仍然是孵化阶段的特征气味。第一天孵化的鱼苗头尾黑色素团的色素沉着和持续扩散。刚孵化出来的幼虫有恐光症。黄色的储备减少,使其能够轻松游泳,大多数在第四天开始进食。卵黄蛋白的储量显著减少,使幼虫能够外源性进食。我们认为,观察到的事件表明存在两个异常关键的参数:使用高质量的饮食和保证清洁优质的水源,满足水产养殖的标准。
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引用次数: 0
Analysis of Nutritional Value and Consumer Behavior Diversification of Milkfish (Chanos sp.) Products From Aquaculture Products By Packaging Modification In MSME Sukolilo, Surabaya 泗水Sukolilo中小微企业包装改造水产养殖遮目鱼产品营养价值及消费者行为多样化分析
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.31382
Patmawati Patmawati, S. Subekti, W. Tjahjaningsih, Ida Ayu Puspitasari, Annisa Nur R. P., Mohamad Akmal A.H., Renyta Andini, W., Aliffiansyah Rizky E
Analisis nilai gizi dan prilaku konsumen merupakan aspek penting untuk peningkatan nilai jual dari produk baru. Perbaikan pada kemasan dan informasi nilai gizi adalah salah satu cara untuk meningkatkan branding sebuah produk dan meningkatkan nilai jual produk terutama di masa pandemic Covid-19. Oleh sebab itu, peneltian ini bertujuan untuk menganalisis nilai gizi dan perilaku konsumen pada produk otak-otak dan bandeng presto yang diolah oleh salah satu UMKM di Surabaya. Metode yang digunakan yaitu dengan analysis nilai gizi produk yang diolah oleh UMKM dlabolatorium dan analisis prilaku konsumen dengan menyebar kuisioner ke 50 responden dengan berbagai latar belakang Pendidikan. Hasil uji analisis nilai gizi kedua produk untuk bandeng presto yaitu kadar air sebesar 61%, kadar abu 7.64%, kadar lemak 6%, dan kadar protein 8.79%. Sedangkan untuk otak-otak bandeng mengandung kadar air 58.20%, kadar abu 3.12%, kadar lemak  13.42%, dan kadar protein 7.69%. Hasil analisis prilaku konsumen menunjukkan bahwa konsumen memilih produk yang menggunakan kemasan yang menarik yang dilengkapi kemasan sekunder dan primer dengan teknologi vakum dibandingkan dengan kemasan mika untuk produk bandeng presto atau daun pisang untuk produk otak. Pengaruh penggunaan kemasan juga tentunya akan bepengaruh terhadap nilai tambah dan umur simpan produk. Hasil riset menunjukan juga bahwa konsumen lebih memilih belanja online sebanyak 94% responden dengan mempertimbangan beberapa informasi seperti nilai gizi yang disajikan dalam kemasan. Oleh sebab itu, penjualan produk diversifikasi perikanan memupnya potensi pemasaran yang lebih luas menggunakan platform online dibanding penjualan langsung dipasar. Hal ini juga akan berpengaruh terhadap omzet dan peningkatan perekonomian UMKM jika terus didampingi dalam pengembangan penjualan secara digital. 
营养价值分析和消费者行为是促进新产品销售价值的重要方面。改善包装和营养价值信息是提高产品品牌和提高产品销售价值的一种方式,尤其是在流行年代的Covid-19。因此,该研究的目的是分析泗水中心一种UMKM (UMKM)生产的小脑产品和旗手的营养价值和消费者行为。使用的方法是对UMKM dlabolalab进行的产品营养价值分析和消费者行为分析,将问卷分发给有不同教育背景的50名受访者。直接营养素两种产品的营养价值分析结果为61%,含盐量为7.4%,脂肪含量为6%,蛋白质含量为8.79%。而ban邓脑的含水率为58.20%,含灰3.12%,脂肪水平为13.42%,蛋白质水平为7.69%。消费者行为分析表明,消费者选择使用具有真空技术的次级和初级包装包装的产品,而不是将mika包装用于脑产品或香蕉叶。包装的使用也将影响产品的附加值和保质期。研究还发现,消费者更喜欢94%的在线购物,将食品包装上的营养价值等信息进行分类。因此,渔业多样化产品的销售使其在在线平台上的营销潜力超过了直接市场销售。这也将影响omzet和UMKM的经济增长,如果继续伴随着数字销售的发展。
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引用次数: 0
Vertical Infections and Prevalence of Viral Nervous Necrosis (VNN) in Milkfish (Chanos chanos) 遮目鱼垂直感染及病毒性神经坏死(VNN)的流行
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.32798
Sang Ayu Made Putri Suryani, N. M. Darmadi, Made Goura Premananda, G. N. Permana
Milkfish juvenile is the leading fishery species in Bali with an export value in 2019. The increase in demand is related to the intensification of aquaculture which causes various diseases, especially Viral Nervous Necrosis (VNN). VNN infection in juveniles has been through water media. The VNN infection in milkfish may occur vertically. This study aims to determine the vertical infection and prevalence of VNN in milkfish. The VNN examination was carried out using the reverse transcription real-time Polymerase Chain Reaction (rRT-PCR) method. This research was conducted at BKIPM Denpasar. Samples were milkfish eggs taken from 15 spawning ponds in Gerogak District, Buleleng Regency. The results showed 3 positive samples from the 15 samples tested. A4 VNN positive sample with Ct 39.29 showed a virus concentration of 146 copies, Sample F positive for VNN with Ct 38.79 showing the 1978 copies of virus concentration, Sample G was positive for VNN with Ct 39.16 showing a virus concentration of 1588. Positive results of VNN examination on milkfish eggs with real time PCR indicates the presence of vertical transmission of VNN. It is also supported by measurement of water quality in milkfish spawning ponds shows the parameters of temperature, DO and pH in optimal and appropriate conditions standard. The results of the observation of clinical symptoms also show the condition of milkfish in a healthy state seen from swimming behavior that does not show symptoms of VNN infection. there are 3 positive samples in sample A4 with a virus concentration of 1,466 copies, sample F with a virus concentration of 1,978 copies and sample G with a virus concentration of 1,588. Detected VNN in eggs milkfish showed vertical VNN infection. Prevalence of infection. Milkfish egg against VNN shows a value of 20% which is included in the frequent infection category.
遮目鱼幼鱼是巴厘岛的主要渔业品种,2019年出口额为出口额。需求的增加与水产养殖的集约化有关,水产养殖引起各种疾病,特别是病毒性神经坏死(VNN)。青少年的VNN感染是通过水媒介传播的。遮目鱼的VNN感染可垂直发生。本研究旨在了解遮目鱼VNN的垂直感染及流行情况。VNN检测采用逆转录实时聚合酶链反应(rRT-PCR)法。这项研究是在BKIPM登巴萨进行的。样本是从布勒连县Gerogak区的15个产卵池中采集的遮目鱼卵。结果显示,15份样本中有3份呈阳性。A4 VNN阳性标本Ct值39.29显示病毒浓度为146个拷贝,F标本VNN阳性,Ct值38.79显示病毒浓度为1978个拷贝,G标本VNN阳性,Ct值39.16显示病毒浓度为1588个拷贝。实时PCR检测遮目鱼卵VNN阳性,提示存在VNN垂直传播。遮目鱼产卵池水质测量结果表明,温度、溶解氧和pH值均处于最佳适宜条件标准。临床症状的观察结果也显示遮目鱼从游泳行为上看处于健康状态,没有出现VNN感染的症状。样本A4中有3个阳性样本,病毒浓度为1466份,样本F中有1978份,样本G中有1588份。目鱼卵中检测到的VNN呈垂直感染。感染流行程度。遮目鱼卵对VNN的感染率为20%,属于频繁感染类别。
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引用次数: 1
Morphometric and Meristic Analysis of Rasbora in East Java Province 东爪哇省Rasbora的形态计量学和美学分析
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.30956
P. Kumaladewi, Mufasirin, N. D. Lastuti, M. A. Alamsjah, W. Darmanto, S. Andriyono
Rasbora (Rasbora sp.) is one of the endemic freshwater fish species in East Java, which is currently being threatened due to environmental degradation, human activities and pollution. In East Java, it is known that there are several species of Rasbora (Rasbora sp.). Domestication is one method to preserve Rasbora in East Java, preceded by identifying potential species of Rasbora sp. from the natural catchment. This study aims to determine the diversity of Rasbora sp in East Java. Samples were taken at five different locations, specifically: Nganjuk (Brantas Watersheds), Ngawi (Bengawan Solo Watersheds, Silowo Springs Tuban, Umbulan Springs Pasuruan and the Setail Watersheds Banyuwangi. The main parameters observed were morphology and morphometry. The morphological analysis used identification guidelines, while morphometric data were obtained through 15 parameters measurement then analyzed using one-way Anova test on SPSS 25 software. Based on the morphological and morphometric analysis, it is known that the species of Rasbora in Nganjuk and Ngawi regencies are Rasbora argyrotaenia and the species of Rasbora in Tuban, Pasuruan and Banyuwangi regencies are Rasbora lateristriata. One-way Anova test showed Rasbora from the five locations were significantly different based on the result value of p<0.05.
Rasbora (Rasbora sp.)是东爪哇特有的淡水鱼种之一,目前由于环境退化、人类活动和污染而受到威胁。在东爪哇,已知有几种拉斯博拉(拉斯博拉sp.)。驯化是保护东爪哇拉斯博拉的一种方法,在驯化之前要从自然集水区确定拉斯博拉属的潜在物种。本研究旨在确定东爪哇省拉氏蝇的多样性。在五个不同的地点采集了样本,具体来说是:恩甘珠克(布兰塔斯流域)、恩加维(班加万索罗流域)、西洛沃泉图班、翁布兰泉帕苏鲁和塞泰尔流域班尤旺吉。观察的主要参数是形态学和形态计量学。形态学分析采用鉴定指南,形态计量学数据通过15个参数测量获得,然后使用SPSS 25软件进行单因素方差分析。通过形态学和形态测定学分析,认为位于Nganjuk和Ngawi地区的拉斯博拉属argyrotaenia,而位于Tuban、Pasuruan和Banyuwangi地区的拉斯博拉属Rasbora lateristriata。单因素方差分析显示,5个地点的Rasbora在p<0.05的基础上差异有统计学意义。
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引用次数: 0
Combination of Nigella sativa and Phyllanthus niruri as An Immunostimulant for The Prevention of White Spot Disease in Litopenaeus vannamei 黑皮草与千叶草联合免疫促进剂预防凡纳滨对虾白斑病的研究
Pub Date : 2022-09-02 DOI: 10.20473/jafh.v11i3.27470
Wida Lesmanawati, W. Manalu, M. Rahminiwati, M. Suprayudi, S. Nuryati
Pacific whiteleg shrimp (Litopenaeus vannamei) is a very important commodity, which accounts for almost 70% of the total world shrimp production. However, this production is still threatened by disease attacks, one of which is caused by white spot disease (WSD). Herbs are widely used as immunostimulants as an effort to prevent diseases. Black cumin (Nigella sativa) and stone breaker (Phyllanthus niruri) have long been known as excellent immunostimulants in human. This study aimed to evaluate the potential of N. sativa and its combination with P. niruri as an immunostimulant in L. vannamei for preventing WSD. Tested shrimp (±3.0 g) were treated by N. sativa (N), P. niruri (P) and the combination of both (1N:1P, 2/3N:1/3P, 3/4N:1/4P, 1/3N:2/3, 3/5N:2/5P, 1/4N: 3/4P, 2/5N:3/5P) through feed for 28 days. They were then infected by white spot syndrome virus (WSSV) and observed for 7 days. The parameters observed included immune response and production performance.  N. sativa was able to reduce the mortality of test shrimp infected with WSSV, with an RPS value of 71%. N. sativa can be combined with P. niruri in the right composition (1/2N:1/2P and 2/3N:1/3P), while the other combinations are antagonistic. The administration of the best treatments in this study, relatively did not affect the value of total hemocyte count, phenoloxidase activity, respiratory burst activity, hemolymph clotting time and production performance of tested shrimp.
太平洋白腿虾(Litopenaeus vannamei)是一种非常重要的商品,几乎占世界虾总产量的70%。然而,这种生产仍然受到疾病发作的威胁,其中一种是由白斑病(WSD)引起的。草药被广泛用作免疫刺激剂,以预防疾病。黑孜然(Nigella sativa)和石竹(Phyllanthus nirui)长期以来一直被认为是人类优秀的免疫刺激剂。本研究旨在评估N.sativa及其与P.nirui联合作为南美白对虾免疫刺激剂预防WSD的潜力。试验虾(±3.0g)用N.sativa(N)、P.nirui(P)和二者的组合(1N:1P、2/3N:1/3P、3/4N:1/4P、1/3N:2/3、3/5N:2/5P、1/4N:3/4P、2/5N:3/5P)通过饲料处理28天。然后用白斑综合征病毒(WSSV)感染它们并观察7天。观察到的参数包括免疫反应和生产性能。N.sativa能够降低感染WSSV的试验虾的死亡率,RPS值为71%。N.sativa可以与P.nirui以合适的组成(1/2N:1/2P和2/3N:1/3P)组合,而其他组合是拮抗的。在本研究中,最佳处理的给药相对不影响受试虾的总血细胞计数、酚氧化酶活性、呼吸爆发活性、血淋巴凝固时间和生产性能。
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引用次数: 1
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Journal of Aquaculture and Fish Health
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