Pub Date : 2019-12-26DOI: 10.54987/jobimb.v7i2.481
Shehab E. Talat, Fatma H. M. Ali, Abdelrahim H. A. Hassan
Beef meat is an animal flesh food that is considered a good diet for consumption as it contains many minerals, vitamins, basic amino acids and essential fatty acids. The people prefer marinated beef meat from a few decades. Microbiological count affection food and especially pathogenic organisms of public health importance. The tenderness and flavour considered as the main factors that affect consumer’s acceptability and satisfaction. World’s beef meat production in 2016 is approximately about 321,000,000 tons. The importance of post marinated beef meat on quality and acceptability with the importance of consumer point of view as the health and diet increases as beef meat is a high source of minerals, protein, vitamins as well as some essential compounds. Samples from meat food were collected from supermarkets, Egypt. This study evaluates the effects of marinade on meat microbial quality as reduction of the microbiological count. Microbial count was carried out by using selective medium and to determine the best marinating method. The results indicated that SDS+1% give best microbial controlling buffer where decreasing bacterial and fungal count to around zero that gave marvellous results.
{"title":"Efficacy of Different Marination Methods on Microbial Quality of Meat","authors":"Shehab E. Talat, Fatma H. M. Ali, Abdelrahim H. A. Hassan","doi":"10.54987/jobimb.v7i2.481","DOIUrl":"https://doi.org/10.54987/jobimb.v7i2.481","url":null,"abstract":"Beef meat is an animal flesh food that is considered a good diet for consumption as it contains many minerals, vitamins, basic amino acids and essential fatty acids. The people prefer marinated beef meat from a few decades. Microbiological count affection food and especially pathogenic organisms of public health importance. The tenderness and flavour considered as the main factors that affect consumer’s acceptability and satisfaction. World’s beef meat production in 2016 is approximately about 321,000,000 tons. The importance of post marinated beef meat on quality and acceptability with the importance of consumer point of view as the health and diet increases as beef meat is a high source of minerals, protein, vitamins as well as some essential compounds. Samples from meat food were collected from supermarkets, Egypt. This study evaluates the effects of marinade on meat microbial quality as reduction of the microbiological count. Microbial count was carried out by using selective medium and to determine the best marinating method. The results indicated that SDS+1% give best microbial controlling buffer where decreasing bacterial and fungal count to around zero that gave marvellous results.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76958667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soils present a biodiversity of bacteria, part of this microflora is cultivable, it can be estimated, characterized and identified. Four zones each comprising three sites were sampled in Brazzaville in the Republic of Congo. Phenotypic characterization, molecular identification and proteolytic capacity of soil bacteria were conducted. One of the factors, the pH was measured, the pH values were between 6 and 7. From the classical microbiology techniques, we counted the total flora of the microorganisms in (CFU / g.103), it is between 47 ± 11.2 and 214 ± 58.2 and that of bacteria of the genus Bacillus from 51.3 ± 5.0 to 74 ± 30.7. Cocci and bacilli were obtained, Gram- and Gram + bacteria distinguished, all bacteria were catalase +, some had sporulation, others not. Thirty-one (31) isolates were phenotypically characterized and 16S rDNA PCR was performed. Ten (10) strains were sequenced and the phylogenetic classification of the identified strains presented. Blastn's research on the 16S rDNA sequences of the different strains shows similarity rates (96.77% -100%) and E. value (9.00E-93 -0.0), these two indices allowed the identification of the strains studied. The percentages of the identified strains are as follows: Bacillus cereus (20%) = MN6 and MN14, Bacillus pumilus (10%) = MN7, Bacillus thuringiensis (10%) = MN12, B sp. (10%) = MN17, Bacillus subtilis (10%) = MN26, Staphylococcus haemolyticus (10%) = MN8, Staphylococcus saprophyticus (10%) = MN21, Staphylococcus sp. (10%) = MN22, Staphylococcus gallinarum (10%) = MN24. The phylogenetic tree shows that the bacteria belong to two main monophyletic genera, the genus Bacillus and the genus Staphylococcus. The proteolytic capacity of Bacillus bacteria was assessed in parallel with growth. The optical density was between (0.8- 0.93) and proteolytic enzyme production between (9-20mm). The parallelism between growth and enzymatic production shows that the two phenomena are distinct.
{"title":"Molecular Identification, Phylogenetic Classification and Proteolytic Capacity of Cultivable Bacteria Isolated from Soils in Brazzaville, Republic of Congo","authors":"Ngo Itsouhou, Nguimbi Etienne, Kayath Aimé Christian, Ampa Raoul","doi":"10.54987/jobimb.v7i2.475","DOIUrl":"https://doi.org/10.54987/jobimb.v7i2.475","url":null,"abstract":"Soils present a biodiversity of bacteria, part of this microflora is cultivable, it can be estimated, characterized and identified. Four zones each comprising three sites were sampled in Brazzaville in the Republic of Congo. Phenotypic characterization, molecular identification and proteolytic capacity of soil bacteria were conducted. One of the factors, the pH was measured, the pH values were between 6 and 7. From the classical microbiology techniques, we counted the total flora of the microorganisms in (CFU / g.103), it is between 47 ± 11.2 and 214 ± 58.2 and that of bacteria of the genus Bacillus from 51.3 ± 5.0 to 74 ± 30.7. Cocci and bacilli were obtained, Gram- and Gram + bacteria distinguished, all bacteria were catalase +, some had sporulation, others not. Thirty-one (31) isolates were phenotypically characterized and 16S rDNA PCR was performed. Ten (10) strains were sequenced and the phylogenetic classification of the identified strains presented. Blastn's research on the 16S rDNA sequences of the different strains shows similarity rates (96.77% -100%) and E. value (9.00E-93 -0.0), these two indices allowed the identification of the strains studied. The percentages of the identified strains are as follows: Bacillus cereus (20%) = MN6 and MN14, Bacillus pumilus (10%) = MN7, Bacillus thuringiensis (10%) = MN12, B sp. (10%) = MN17, Bacillus subtilis (10%) = MN26, Staphylococcus haemolyticus (10%) = MN8, Staphylococcus saprophyticus (10%) = MN21, Staphylococcus sp. (10%) = MN22, Staphylococcus gallinarum (10%) = MN24. The phylogenetic tree shows that the bacteria belong to two main monophyletic genera, the genus Bacillus and the genus Staphylococcus. The proteolytic capacity of Bacillus bacteria was assessed in parallel with growth. The optical density was between (0.8- 0.93) and proteolytic enzyme production between (9-20mm). The parallelism between growth and enzymatic production shows that the two phenomena are distinct.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82282019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-26DOI: 10.54987/jobimb.v7i2.479
Rusnam, B. Gunasekaran, M. K. Sabullah
Numerous publications ignore statistical diagnosing of the nonlinear model utilized, and the data might be nonrandom- an essential necessity for all of the parametric statistical evaluation approaches. In cases where the diagnostic tests demonstrate that the residuals reveal a pattern, then a variety of remedies for example nonparametric analysis or shifting to another model should cure the problem. The subject of this study is test for the randomness of the residual for the Four-parameter Logistic model used in obtaining the IC50 Value for Allivum sativum methanolic extract against Aeromonas hydrophila using the Wald–Wolfowitz runs test. The result shows that the number of runs was 10, the expected number of runs under the assumption of randomness was 5.8, indicating the series of residuals had adequate runs. As the p-value was greater than 0.05, the null hypothesis is not rejected demonstrating no substantial evidence that the residuals were nonrandom, and the residuals represent noise.
{"title":"Test of Randomness of Residuals for the Four-parameter Logistic model used in Obtaining the IC50 Value for Allivum sativum Methanolic Extract Against Aeromonas hydrophila","authors":"Rusnam, B. Gunasekaran, M. K. Sabullah","doi":"10.54987/jobimb.v7i2.479","DOIUrl":"https://doi.org/10.54987/jobimb.v7i2.479","url":null,"abstract":"Numerous publications ignore statistical diagnosing of the nonlinear model utilized, and the data might be nonrandom- an essential necessity for all of the parametric statistical evaluation approaches. In cases where the diagnostic tests demonstrate that the residuals reveal a pattern, then a variety of remedies for example nonparametric analysis or shifting to another model should cure the problem. The subject of this study is test for the randomness of the residual for the Four-parameter Logistic model used in obtaining the IC50 Value for Allivum sativum methanolic extract against Aeromonas hydrophila using the Wald–Wolfowitz runs test. The result shows that the number of runs was 10, the expected number of runs under the assumption of randomness was 5.8, indicating the series of residuals had adequate runs. As the p-value was greater than 0.05, the null hypothesis is not rejected demonstrating no substantial evidence that the residuals were nonrandom, and the residuals represent noise.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87893000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.446
N. Raihan, S. Abbasiliasi, J. Tan, M. Masomian, T. Ibrahim, A. Ariff, S. Mustafa
This study aimed at extraction of protein from the wastes of the threadfin bream, Nemipterus japonicus using pH shift solubilization and precipitation processes and evaluation of the influencing factors on protein extraction. Towards this objective, the wastes of head, skin and internal organs were collected, and their nutritional composition evaluated. Furthermore, the ratio of waste: water for protein extraction was screened and the effect of pH, centrifugation speed and time on protein solubility rate, the amino acid composition and the molecular weight of proteins via SDS-PAGE were determined. Results demonstrated that moisture was the highest percentage (~70-78%) of all wastes. Highest protein content (19.67±1.10%), fat content (1.81±0.09%) and carbohydrate content (4.43±0.23%) were observed in skin, head and internal organs, respectively. The optimum ratio of waste: water for protein extraction is 1:9 for head, 1:8 for internal organs and 1:6 for skin. The protein extraction efficiency for all three samples were high at pH 3 and pH 12. Protein solubility increased with increased centrifugation speed up to 10,000 ×g, and there was no significant difference (P>0.05) between the protein solubility at 10,000 ×g and 20’000 ×g. Furthermore, there was significant difference (P<0.05) between protein solubility with increased centrifugation time. Alkaline process showed a remarkably higher amino acid content as compared to that of acid version. Glutamic acid and lysine were found higher compared to other amino acids. The molecular weight of proteins isolated in this study were low (<100 KDa).
{"title":"pH Shift Solubilization and Precipitation Protein Extraction from the Wastes of the Threadfin Bream, Nemipterus japonicus","authors":"N. Raihan, S. Abbasiliasi, J. Tan, M. Masomian, T. Ibrahim, A. Ariff, S. Mustafa","doi":"10.54987/jobimb.v7i1.446","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.446","url":null,"abstract":"This study aimed at extraction of protein from the wastes of the threadfin bream, Nemipterus japonicus using pH shift solubilization and precipitation processes and evaluation of the influencing factors on protein extraction. Towards this objective, the wastes of head, skin and internal organs were collected, and their nutritional composition evaluated. Furthermore, the ratio of waste: water for protein extraction was screened and the effect of pH, centrifugation speed and time on protein solubility rate, the amino acid composition and the molecular weight of proteins via SDS-PAGE were determined. Results demonstrated that moisture was the highest percentage (~70-78%) of all wastes. Highest protein content (19.67±1.10%), fat content (1.81±0.09%) and carbohydrate content (4.43±0.23%) were observed in skin, head and internal organs, respectively. The optimum ratio of waste: water for protein extraction is 1:9 for head, 1:8 for internal organs and 1:6 for skin. The protein extraction efficiency for all three samples were high at pH 3 and pH 12. Protein solubility increased with increased centrifugation speed up to 10,000 ×g, and there was no significant difference (P>0.05) between the protein solubility at 10,000 ×g and 20’000 ×g. Furthermore, there was significant difference (P<0.05) between protein solubility with increased centrifugation time. Alkaline process showed a remarkably higher amino acid content as compared to that of acid version. Glutamic acid and lysine were found higher compared to other amino acids. The molecular weight of proteins isolated in this study were low (<100 KDa).","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73285218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.444
Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, R. Jamal, M. R. Mohd Idris, N. Ismail, W. F. Wan Jamaludin, S. F. Abd Wahid, Muaatamarulain Mustangin, Nur Maya Sabrina Tizen Laim
Per ARNT SIM domain containing 1 (PASD1) protein belongs to the Cancer Testis Antigen (CTA) family. It shows restricted expression in normal tissues but are highly expressed in cancer tissues. This study aims to further investigate PASD1 expression in Malaysian hematological malignancies patients as a potential biomarker for disease progression and vaccine development. Formalin-fixed Paraffin-Embedded (FFPE) tissue specimens from hematological malignancies patients were labeled with anti-PASD1-1 and anti-PASD1-2 antibodies using immunohistochemistry method. Our results show that among DLBCL patients, 7 samples were positive for PASD1-1, 2 samples were positive for PASD1-2 while 3 samples were positive for both of the antibodies. In addition, only 1 sample from T-cell Lymphoblastic Lymphoma (TLL) showed positive staining with PASD1-1. Other classes of hematological malignancies did not show any positive staining with either antibody. Antibody PASD1-1 stained the membrane and cytoplasm of the tumor cells strongly. Moderate nuclear labeling was also observed in some cases of DLBCL with PASD1-2. PASD1-1 staining was more frequent compared to PASD1-2 in most of the samples. Positive PASD1 staining was observed in patients with age range between 36-71 years old, higher in male cases than female by 54% and higher in Malay patients compared to Chinese by 77%. Due to its frequency, the PASD1_v1 protein may play a role in the DLBCL initiation and progression. A higher number of PASD1 staining in DLBCL samples compared to other lymphoma subtypes may suggest that PASD1 may represent a potential for DLBCL subtyping marker.
{"title":"PASD1 Expression in Malaysian Hematological Malignancies Patients","authors":"Nor Adzimah Johdi, Hanif Zulkhairi Mohd Said, R. Jamal, M. R. Mohd Idris, N. Ismail, W. F. Wan Jamaludin, S. F. Abd Wahid, Muaatamarulain Mustangin, Nur Maya Sabrina Tizen Laim","doi":"10.54987/jobimb.v7i1.444","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.444","url":null,"abstract":"Per ARNT SIM domain containing 1 (PASD1) protein belongs to the Cancer Testis Antigen (CTA) family. It shows restricted expression in normal tissues but are highly expressed in cancer tissues. This study aims to further investigate PASD1 expression in Malaysian hematological malignancies patients as a potential biomarker for disease progression and vaccine development. Formalin-fixed Paraffin-Embedded (FFPE) tissue specimens from hematological malignancies patients were labeled with anti-PASD1-1 and anti-PASD1-2 antibodies using immunohistochemistry method. Our results show that among DLBCL patients, 7 samples were positive for PASD1-1, 2 samples were positive for PASD1-2 while 3 samples were positive for both of the antibodies. In addition, only 1 sample from T-cell Lymphoblastic Lymphoma (TLL) showed positive staining with PASD1-1. Other classes of hematological malignancies did not show any positive staining with either antibody. Antibody PASD1-1 stained the membrane and cytoplasm of the tumor cells strongly. Moderate nuclear labeling was also observed in some cases of DLBCL with PASD1-2. PASD1-1 staining was more frequent compared to PASD1-2 in most of the samples. Positive PASD1 staining was observed in patients with age range between 36-71 years old, higher in male cases than female by 54% and higher in Malay patients compared to Chinese by 77%. Due to its frequency, the PASD1_v1 protein may play a role in the DLBCL initiation and progression. A higher number of PASD1 staining in DLBCL samples compared to other lymphoma subtypes may suggest that PASD1 may represent a potential for DLBCL subtyping marker.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91293568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.447
Muhamad Amin Jahari, S. Mustafa, Y. A. Manap, Dhilia Udie Lamasudin, M. Roslan
Probiotic bacteria are utilized in aquaculture as they exert a crucial function in promoting and maintaining the fish health. Probiotic strains should be present in a viable form during consumption and throughout the gastrointestinal tract for maximum health benefits. Many reports stated that there is poor survival of probiotic in products containing free probiotic cells. Providing probiotic living cells with physical barrier to resist adverse environmental conditions is therefore an approach currently receiving considerable interest. In this study, Lactobacillus plantarum was encapsulated with mannan and sodium alginate to increase probiotic viability. Response surface methodology (RSM) based on Box-Behnken design was used to optimize the encapsulation process with mannan concentration (5 to 30 % w/v) and sodium alginate concentration (1 to 5 % w/v) as the independent variables evaluated. According to the regression coefficients and significance of the polynomial model, the optimum encapsulation parameters were as follows: 24.73 % w/v mannan; 1.6 % w/v sodium alginate. Under these conditions of encapsulation, the total cell production of the Lactobacillus plantarum was increased to 5.3 (108 CFU/g) as compared to the free cell culture, 3.2 (108 CFU/g).
{"title":"Encapsulation of Lactobacillus plantarum with Mannan and Sodium Alginate Improves its Cell Production","authors":"Muhamad Amin Jahari, S. Mustafa, Y. A. Manap, Dhilia Udie Lamasudin, M. Roslan","doi":"10.54987/jobimb.v7i1.447","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.447","url":null,"abstract":"Probiotic bacteria are utilized in aquaculture as they exert a crucial function in promoting and maintaining the fish health. Probiotic strains should be present in a viable form during consumption and throughout the gastrointestinal tract for maximum health benefits. Many reports stated that there is poor survival of probiotic in products containing free probiotic cells. Providing probiotic living cells with physical barrier to resist adverse environmental conditions is therefore an approach currently receiving considerable interest. In this study, Lactobacillus plantarum was encapsulated with mannan and sodium alginate to increase probiotic viability. Response surface methodology (RSM) based on Box-Behnken design was used to optimize the encapsulation process with mannan concentration (5 to 30 % w/v) and sodium alginate concentration (1 to 5 % w/v) as the independent variables evaluated. According to the regression coefficients and significance of the polynomial model, the optimum encapsulation parameters were as follows: 24.73 % w/v mannan; 1.6 % w/v sodium alginate. Under these conditions of encapsulation, the total cell production of the Lactobacillus plantarum was increased to 5.3 (108 CFU/g) as compared to the free cell culture, 3.2 (108 CFU/g).","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87600862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.449
K. I. Karamba, H. Yakasai
The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as detergent in industrial washing, which results in the high effluent level of this contaminant. SDS as anionic surfactant is ubiquitously toxic to the aquatic ecosystem. In this study, the potentials of a previously isolated molybdenum-reducing Bacillus amyloliquefaciens strain KIK-12 to degrade and utilize SDS as sole source of carbon was investigated. The bacterium grew optimally at pH between 6.0 and 7.0, temperature between 30 and 45 °C in 1 g/L SDS as the sole source of carbon, with ammonium sulphate (1 % w/v) as the best nitrogen source. The growth characteristics of strain KIK-12 on various concentrations of SDS (as a carbon source) reveals optimum growth 500 mg/L but was able to tolerate and grow at 1500 mg/L. However, concentrations higher than this results in growth termination. Heavy metals such as mercury, silver and copper significantly inhibit growth of strain KIK-12 on SDS. The ability of this bacterium to tolerate and detoxify multiple toxicants makes it suitable for their bioremediation.
{"title":"Growth Characterization of Bacillus amyloliquefaciens strain KIK-12 on SDS","authors":"K. I. Karamba, H. Yakasai","doi":"10.54987/jobimb.v7i1.449","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.449","url":null,"abstract":"The pollution caused by sodium dodecyl sulfate (SDS) arise mainly from its utilization as detergent in industrial washing, which results in the high effluent level of this contaminant. SDS as anionic surfactant is ubiquitously toxic to the aquatic ecosystem. In this study, the potentials of a previously isolated molybdenum-reducing Bacillus amyloliquefaciens strain KIK-12 to degrade and utilize SDS as sole source of carbon was investigated. The bacterium grew optimally at pH between 6.0 and 7.0, temperature between 30 and 45 °C in 1 g/L SDS as the sole source of carbon, with ammonium sulphate (1 % w/v) as the best nitrogen source. The growth characteristics of strain KIK-12 on various concentrations of SDS (as a carbon source) reveals optimum growth 500 mg/L but was able to tolerate and grow at 1500 mg/L. However, concentrations higher than this results in growth termination. Heavy metals such as mercury, silver and copper significantly inhibit growth of strain KIK-12 on SDS. The ability of this bacterium to tolerate and detoxify multiple toxicants makes it suitable for their bioremediation.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"162 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86294363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.453
M. Manogaran, H. Yakasai, K. I. Karamba
Cyanide is extremely toxic to living organisms. It is utilized in industries for gold and silver extraction, pharmaceuticals, plastic processing, electroplating, and agricultural chemistry. Cyanide can persevere for a long time in the soil and its bio-degradation is the best economical practice. A formerly isolated cyanide-degrading bacterium Azotobacter vinelandii exhibited substrate inhibition to degradation rate. Significant degradation inhibition constants were achieved reliably by means of non-linear regression modeling of the degradation rate profile utilizing models for substrate inhibition like Haldane, Teissier-Edward, Monod, Yano and Koga, Luong, Edward (Webb) Luong and Aiba models. Aiba model was selected as the top model established on statistical assessments like root mean square error, adjusted coefficient of determination, bias factor and accuracy factor. The calculated values for the Aiba constants qmax (the maximum specific substrate degradation rate (h−1), Ks (concentration of substrate at the half maximal degradation rate (mM) and Ki (inhibition constant (mM)) were 0.060 (95% CI, 0.024 to 0.096), 0.302 (95% CI, 0.381 to 0.222) and 0.953 (95% CI, 0.568 to 1.338), respectively. The novel constants gotten from the modeling application can be valuable for advanced secondary modeling implicating the influence of media conditions and some other dynamics on cyanide biodegradation by this bacterium.
{"title":"Kinetics of Cyanide Degradation by Azotobacter vinelandii","authors":"M. Manogaran, H. Yakasai, K. I. Karamba","doi":"10.54987/jobimb.v7i1.453","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.453","url":null,"abstract":"Cyanide is extremely toxic to living organisms. It is utilized in industries for gold and silver extraction, pharmaceuticals, plastic processing, electroplating, and agricultural chemistry. Cyanide can persevere for a long time in the soil and its bio-degradation is the best economical practice. A formerly isolated cyanide-degrading bacterium Azotobacter vinelandii exhibited substrate inhibition to degradation rate. Significant degradation inhibition constants were achieved reliably by means of non-linear regression modeling of the degradation rate profile utilizing models for substrate inhibition like Haldane, Teissier-Edward, Monod, Yano and Koga, Luong, Edward (Webb) Luong and Aiba models. Aiba model was selected as the top model established on statistical assessments like root mean square error, adjusted coefficient of determination, bias factor and accuracy factor. The calculated values for the Aiba constants qmax (the maximum specific substrate degradation rate (h−1), Ks (concentration of substrate at the half maximal degradation rate (mM) and Ki (inhibition constant (mM)) were 0.060 (95% CI, 0.024 to 0.096), 0.302 (95% CI, 0.381 to 0.222) and 0.953 (95% CI, 0.568 to 1.338), respectively. The novel constants gotten from the modeling application can be valuable for advanced secondary modeling implicating the influence of media conditions and some other dynamics on cyanide biodegradation by this bacterium.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78158676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.448
Rusnam, Syafrawati, N. Gusmanizar
Microorganisms from thirty soil samples from various locations in West Sumatera was screened for their ability to grow on xenobiotics such as acrylamide, propionamide, polyethylene glycol (PEG) 1000, Sodium dodecyl Sulfate (SDS), Sodium dodecylbenzene sulfonates (SDBS), diesel and phenol. The screening media contain all of these xenobiotics as carbon sources, ammonium sulphate as nitrogen sources and trace elements. The growth of these microorganisms on the xenobiotics was monitored at A600 nm on a microplate. Of the isolates screened, the number of bacterial isolates than can grow on acrylamide, propionamide, polyethylene glycol (PEG) 1000, SDS, SDBS, diesel and phenol were 23, 22, 13, 17, 3, 17 and 17, respectively, all as carbon sources. These xenobiotics-degrading isolates can be further utilized in the future as bioremediation agent.
{"title":"Screening of Xenobiotics-degrading Microorganisms from Sumatera’s Soil","authors":"Rusnam, Syafrawati, N. Gusmanizar","doi":"10.54987/jobimb.v7i1.448","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.448","url":null,"abstract":"Microorganisms from thirty soil samples from various locations in West Sumatera was screened for their ability to grow on xenobiotics such as acrylamide, propionamide, polyethylene glycol (PEG) 1000, Sodium dodecyl Sulfate (SDS), Sodium dodecylbenzene sulfonates (SDBS), diesel and phenol. The screening media contain all of these xenobiotics as carbon sources, ammonium sulphate as nitrogen sources and trace elements. The growth of these microorganisms on the xenobiotics was monitored at A600 nm on a microplate. Of the isolates screened, the number of bacterial isolates than can grow on acrylamide, propionamide, polyethylene glycol (PEG) 1000, SDS, SDBS, diesel and phenol were 23, 22, 13, 17, 3, 17 and 17, respectively, all as carbon sources. These xenobiotics-degrading isolates can be further utilized in the future as bioremediation agent.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84141197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-31DOI: 10.54987/jobimb.v7i1.455
Che Nur Mazadillina Che Zahari, N. S. Azmi, Muhammad Zaki Ramli
Glycosaminoglycans (GAGs) are usually found in mammals or invertebrates organisms. Since there are many potential active ingredients in plants that have not been investigated till dates, this review will be discussing the plants’ potential to be the alternative for GAGs source. The study will enlighten the correlation between two crucial properties, anticoagulant and anticancer properties. This is because these two properties are usually found in organisms with GAGs presence. GAGs have obviously played an important role to elicit these two properties within an organism’s biological system. The study found that based on previous researches investigations, plants have the potential to have GAGs which then act as replacement of anticoagulant carrying anticancer properties.
{"title":"Glycosaminoglycans From Plant Sources and The Potential Uses as Anticoagulant and Anticancer Agents","authors":"Che Nur Mazadillina Che Zahari, N. S. Azmi, Muhammad Zaki Ramli","doi":"10.54987/jobimb.v7i1.455","DOIUrl":"https://doi.org/10.54987/jobimb.v7i1.455","url":null,"abstract":"Glycosaminoglycans (GAGs) are usually found in mammals or invertebrates organisms. Since there are many potential active ingredients in plants that have not been investigated till dates, this review will be discussing the plants’ potential to be the alternative for GAGs source. The study will enlighten the correlation between two crucial properties, anticoagulant and anticancer properties. This is because these two properties are usually found in organisms with GAGs presence. GAGs have obviously played an important role to elicit these two properties within an organism’s biological system. The study found that based on previous researches investigations, plants have the potential to have GAGs which then act as replacement of anticoagulant carrying anticancer properties.","PeriodicalId":15132,"journal":{"name":"Journal of Biochemistry, Microbiology and Biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74575829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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