Manuela Gallón Bedoya, Misael Cortés Rodríguez, J. Gil G, Roberto Lemus Mondaca
BACKGROUND: There is consumer interest in berries due to their nutritional properties, especially biologically active compounds. Spray drying technologies improve products stability and shelf life, and the agglomeration process subsequently enhances powder instantaneousness and flow properties. OBJECTIVE: The aim was to evaluate the effect of the fluidized bed agglomeration process on flow, reconstitution, and antioxidant properties of cape gooseberry, strawberry, and blackberry powder mix obtained by spray drying and a water and vitamin C binding solution. METHODS: A face-centered central composite design with 15 experiments was used; independent variables were fluidization temperature (T), binder solution atomization pressure (P), and process time (t), while dependent variables were moisture, solubility, wettability, particle size, antioxidant capacity, total phenols, Vit. C, β-carotenes (β-car), and total anthocyanins. RESULTS: The best process conditions from experimental optimization were T = 70 °C, P = 1.1 bar, and t = 29.1 min. This agglomerated product had low moisture values, good solubility, and 0.5 min wettability; the values for Vit. C = 246.4±3.4 mg/100 g db, β-car = 4.0±0.5 mg/100 g db, and total anthocyanins = 230.7±3.0 mg C3G/100 g db were noteworthy. CONCLUSIONS: An agglomerate berry-based powder product was obtained with 100% Vit. C RDA (Recommended Dietary Allowances) and 15% vitamin A RDA. Flow and instantaneousness properties of the agglomerated powder also significantly improved with the applied process.
{"title":"Impact of the agglomeration process on the flow, reconstitution, and antioxidant properties of an Andean berry powder mix","authors":"Manuela Gallón Bedoya, Misael Cortés Rodríguez, J. Gil G, Roberto Lemus Mondaca","doi":"10.3233/jbr-211563","DOIUrl":"https://doi.org/10.3233/jbr-211563","url":null,"abstract":"BACKGROUND: There is consumer interest in berries due to their nutritional properties, especially biologically active compounds. Spray drying technologies improve products stability and shelf life, and the agglomeration process subsequently enhances powder instantaneousness and flow properties. OBJECTIVE: The aim was to evaluate the effect of the fluidized bed agglomeration process on flow, reconstitution, and antioxidant properties of cape gooseberry, strawberry, and blackberry powder mix obtained by spray drying and a water and vitamin C binding solution. METHODS: A face-centered central composite design with 15 experiments was used; independent variables were fluidization temperature (T), binder solution atomization pressure (P), and process time (t), while dependent variables were moisture, solubility, wettability, particle size, antioxidant capacity, total phenols, Vit. C, β-carotenes (β-car), and total anthocyanins. RESULTS: The best process conditions from experimental optimization were T = 70 °C, P = 1.1 bar, and t = 29.1 min. This agglomerated product had low moisture values, good solubility, and 0.5 min wettability; the values for Vit. C = 246.4±3.4 mg/100 g db, β-car = 4.0±0.5 mg/100 g db, and total anthocyanins = 230.7±3.0 mg C3G/100 g db were noteworthy. CONCLUSIONS: An agglomerate berry-based powder product was obtained with 100% Vit. C RDA (Recommended Dietary Allowances) and 15% vitamin A RDA. Flow and instantaneousness properties of the agglomerated powder also significantly improved with the applied process.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42434046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Zakaria, P. Vit, Adi Wijaya, A. H. Ahmad, Zahiruddin Othman, B. Mezzetti
Blueberries, strawberries and raspberries are soft fruits marketed worldwide, with unique sensory, nutritional and biological properties. This bibliometric comparative analysis aims to analyse the published scientific research in order to provide a valuable and important reference for berry growers, the fruit industry, health personnel and scientists in berry research communities. The Scopus database was utilised for bibliometric analysis of scientific articles on blueberry, raspberry and strawberry fruits published in English in the last decade (2012 to 2021). The publishing trends in blueberry, raspberry, and strawberry research were increased, with strawberry research showing the most significant increase. The top country and institutions researching the three berries were in the United States, followed by China. The top journal was Hortscience for blueberries and Scientia Horticulturae for raspberry and strawberry. The top authors were Drummond FA, Stoner GD and Peres NA for blueberry, raspberry and strawberry, respectively. The most frequent keywords concerning these berries were analyzed and clustered in thematic groups. Core keywords related to functional foods, medicinal properties, as well as fruit quality and gene expression themes were identified. Apart from medicinal and fruit quality themes, the biological control theme was also identified to be relevant in future research.
{"title":"Evolution of blueberry (Vaccinium corymbosum L), raspberry (Rubus idaeus L) and strawberry (Fragaria x ananassa Duch.) research: 2012–2021","authors":"R. Zakaria, P. Vit, Adi Wijaya, A. H. Ahmad, Zahiruddin Othman, B. Mezzetti","doi":"10.3233/jbr-220001","DOIUrl":"https://doi.org/10.3233/jbr-220001","url":null,"abstract":"Blueberries, strawberries and raspberries are soft fruits marketed worldwide, with unique sensory, nutritional and biological properties. This bibliometric comparative analysis aims to analyse the published scientific research in order to provide a valuable and important reference for berry growers, the fruit industry, health personnel and scientists in berry research communities. The Scopus database was utilised for bibliometric analysis of scientific articles on blueberry, raspberry and strawberry fruits published in English in the last decade (2012 to 2021). The publishing trends in blueberry, raspberry, and strawberry research were increased, with strawberry research showing the most significant increase. The top country and institutions researching the three berries were in the United States, followed by China. The top journal was Hortscience for blueberries and Scientia Horticulturae for raspberry and strawberry. The top authors were Drummond FA, Stoner GD and Peres NA for blueberry, raspberry and strawberry, respectively. The most frequent keywords concerning these berries were analyzed and clustered in thematic groups. Core keywords related to functional foods, medicinal properties, as well as fruit quality and gene expression themes were identified. Apart from medicinal and fruit quality themes, the biological control theme was also identified to be relevant in future research.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42657426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camilo Andrés Reyes-Álvarez, J. Gamboa‐Santos, M. C. Lanari
BACKGROUND: Arazá, a tropical berry rich in thermolabile antioxidants (ascorbic-acid/phenolics/flavonoids/carotenoids), is a potential ingredient for preparing functional foods; however, its high perishability hinders its industrial application. Osmotic-dehydration (OD), a processing method that operates at low temperature ranges, may be adequate for improving arazá's shelf-life without reducing its palatability/nutritional quality. Physicochemical and nutritional properties depend on the time/temperature/osmotic-solution concentration applied; therefore, parameters optimization is required. Processing selection must also consider treatment effect on antioxidants composition/activity/bioaccessibility in the gastro-intestinal (GI) tract, key factors in bioactives health benefits. OBJECTIVES: a) Optimize OD duration/temperature/sucrose concentration to maximize process efficiency/total-polyphenol content/overall-acceptability using principal component analysis and Response-Surface-Methodology/Desirability-Function b) Determine OD effect on antioxidant content/activity/bioaccessibilities before and after gastro-intestinal digestion. RESULTS AND CONCLUSIONS: Processing conditions have opposite effects on sensory and chemical properties; reaching antioxidant content/activity highest levels in the osmo-dehydrated fruit required 60 min/20°Bx; whereas, the best organoleptic scores demanded 180 min/60°Bx. Although osmotic-drying reduced the fruit's antioxidant content/activity 39–76%, the process improved antioxidants bioaccessibilities, since these values were: 63–85% (untreated fruit) and 72–90% (osmodehydrated arazá (ODA)), whereas the activity retention levels were 67–76% (untreated fruit/ODA).
{"title":"Optimization of the processing conditions for producing osmo-dehydrated arazá (Eugenia stipitata, McVaugh) and evaluation of its antioxidant content and activity","authors":"Camilo Andrés Reyes-Álvarez, J. Gamboa‐Santos, M. C. Lanari","doi":"10.3233/jbr-211514","DOIUrl":"https://doi.org/10.3233/jbr-211514","url":null,"abstract":"BACKGROUND: Arazá, a tropical berry rich in thermolabile antioxidants (ascorbic-acid/phenolics/flavonoids/carotenoids), is a potential ingredient for preparing functional foods; however, its high perishability hinders its industrial application. Osmotic-dehydration (OD), a processing method that operates at low temperature ranges, may be adequate for improving arazá's shelf-life without reducing its palatability/nutritional quality. Physicochemical and nutritional properties depend on the time/temperature/osmotic-solution concentration applied; therefore, parameters optimization is required. Processing selection must also consider treatment effect on antioxidants composition/activity/bioaccessibility in the gastro-intestinal (GI) tract, key factors in bioactives health benefits. OBJECTIVES: a) Optimize OD duration/temperature/sucrose concentration to maximize process efficiency/total-polyphenol content/overall-acceptability using principal component analysis and Response-Surface-Methodology/Desirability-Function b) Determine OD effect on antioxidant content/activity/bioaccessibilities before and after gastro-intestinal digestion. RESULTS AND CONCLUSIONS: Processing conditions have opposite effects on sensory and chemical properties; reaching antioxidant content/activity highest levels in the osmo-dehydrated fruit required 60 min/20°Bx; whereas, the best organoleptic scores demanded 180 min/60°Bx. Although osmotic-drying reduced the fruit's antioxidant content/activity 39–76%, the process improved antioxidants bioaccessibilities, since these values were: 63–85% (untreated fruit) and 72–90% (osmodehydrated arazá (ODA)), whereas the activity retention levels were 67–76% (untreated fruit/ODA).","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46514817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. L. Ordóñez, Rodrigo Pérez, A. Barriga, I. Seguel, Paola Guzman, M. C. Zúñiga, C. Delporte
BACKGROUND: Sustained hyperglycemia leads to multiple health complications associated with oxidative balance and metabolic pathways alterations. Current treatments for hyperglycemia are not entirely effective thus, the identification of natural products from food sources, such as Ugni molinae berries, to reduce hyperglycemia and prevent the deleterious effect of oxidative stress is attractive to develop new therapeutics. OBJECTIVE: Our aim was to evaluate the antioxidant capacity and the inhibitory activity on α-glucosidase and glycogen phosphorylase A of polyphenolic extracts from different genotypes of U. molinae berries and to comparatively analyze their polyphenolic profile. METHODS: Berry extraction was performed by exhaustive maceration with increasing-polarity solvents. The antioxidant capacity, and inhibitory activity on enzymes were analyzed by different spectrophotometric methods. Moreover, the chemical profile of bioactive extracts was comparatively evaluated through LC-MS. RESULTS: 16 semi-purified extracts were obtained and showed antioxidant capacity and inhibitory activity on the evaluated enzymes. Moreover, the chemical analysis showed differences in phenolic profile among the extracts and, particularly, the acetonic extracts were more concentrated in phenolic compounds, which is associated with the more potent activities. CONCLUSIONS: Our results indicate that murtilla berries’ acetonic extracts include a mixture of phenolic compounds that inhibit the activity of two enzymes related to carbohydrate metabolism and have a promising antioxidant capacity.
{"title":"Comparative study of antioxidant and inhibitory activity on α-glucosidase and glycogen phosphorylase A of berry extracts from Ugni molinae genotypes","authors":"J. L. Ordóñez, Rodrigo Pérez, A. Barriga, I. Seguel, Paola Guzman, M. C. Zúñiga, C. Delporte","doi":"10.3233/jbr-211552","DOIUrl":"https://doi.org/10.3233/jbr-211552","url":null,"abstract":"BACKGROUND: Sustained hyperglycemia leads to multiple health complications associated with oxidative balance and metabolic pathways alterations. Current treatments for hyperglycemia are not entirely effective thus, the identification of natural products from food sources, such as Ugni molinae berries, to reduce hyperglycemia and prevent the deleterious effect of oxidative stress is attractive to develop new therapeutics. OBJECTIVE: Our aim was to evaluate the antioxidant capacity and the inhibitory activity on α-glucosidase and glycogen phosphorylase A of polyphenolic extracts from different genotypes of U. molinae berries and to comparatively analyze their polyphenolic profile. METHODS: Berry extraction was performed by exhaustive maceration with increasing-polarity solvents. The antioxidant capacity, and inhibitory activity on enzymes were analyzed by different spectrophotometric methods. Moreover, the chemical profile of bioactive extracts was comparatively evaluated through LC-MS. RESULTS: 16 semi-purified extracts were obtained and showed antioxidant capacity and inhibitory activity on the evaluated enzymes. Moreover, the chemical analysis showed differences in phenolic profile among the extracts and, particularly, the acetonic extracts were more concentrated in phenolic compounds, which is associated with the more potent activities. CONCLUSIONS: Our results indicate that murtilla berries’ acetonic extracts include a mixture of phenolic compounds that inhibit the activity of two enzymes related to carbohydrate metabolism and have a promising antioxidant capacity.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49450222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Blueberry fruits contain large amounts of phenolic compounds derived from the phenylpropanoid pathway. Their biosynthesis is complex, involving many enzymes. OBJECTIVE: We sought to investigate the content of phenylpropanoid-derived compounds and identify key genes involved in the phenylpropanoid metabolite pathway during half-highbush blueberry fruit development. METHODS: Phenylpropanoid metabolite contents were determined by high-performance liquid chromatography (HPLC) and spectrophotometry. Gene expression was examined through reverse-transcription PCR. RESULTS: Phloretin, chlorogenic acid, total flavonol, quercetin, catechin, and proanthocyanidin contents were highest in small-sized green fruits; myricetin and epicatechin contents were highest in pink fruits; and lignin and anthocyanin were highest in blue fruits. Genes from the 4-coumarate CoA ligase (4CL) family regulate the biosynthesis of phenylpropanoid metabolites. Phenylalanine ammonia-lyase (PAL) and cinnamoyl-CoA reductase (CCR) are key genes in the lignin biosynthetic pathway. Flavonol synthase (FLS) is a key gene affecting total flavonols and the quercetin biosynthetic pathway. PAL and chalcone isomerase (CHI) are key genes in the epicatechin and anthocyanin biosynthetic pathways, respectively. CONCLUSION: Phenylpropanoid metabolites are regulated by multiple genes from the same or different families. Enzymes in different metabolic pathways compete for precursors to form a complex regulatory network for phenylalanine metabolism.
{"title":"Key genes for phenylpropanoid metabolite biosynthesis during half-highbush blueberry (Vaccinium angustifolium×Vaccinium corymbosum) fruit development","authors":"Bofei Yang, Yan-Hai Song, Yuening Li, Xianglong Wang, Qingxun Guo, Lianxia Zhou, Yang Zhang, Chunyu Zhang","doi":"10.3233/jbr-211554","DOIUrl":"https://doi.org/10.3233/jbr-211554","url":null,"abstract":"BACKGROUND: Blueberry fruits contain large amounts of phenolic compounds derived from the phenylpropanoid pathway. Their biosynthesis is complex, involving many enzymes. OBJECTIVE: We sought to investigate the content of phenylpropanoid-derived compounds and identify key genes involved in the phenylpropanoid metabolite pathway during half-highbush blueberry fruit development. METHODS: Phenylpropanoid metabolite contents were determined by high-performance liquid chromatography (HPLC) and spectrophotometry. Gene expression was examined through reverse-transcription PCR. RESULTS: Phloretin, chlorogenic acid, total flavonol, quercetin, catechin, and proanthocyanidin contents were highest in small-sized green fruits; myricetin and epicatechin contents were highest in pink fruits; and lignin and anthocyanin were highest in blue fruits. Genes from the 4-coumarate CoA ligase (4CL) family regulate the biosynthesis of phenylpropanoid metabolites. Phenylalanine ammonia-lyase (PAL) and cinnamoyl-CoA reductase (CCR) are key genes in the lignin biosynthetic pathway. Flavonol synthase (FLS) is a key gene affecting total flavonols and the quercetin biosynthetic pathway. PAL and chalcone isomerase (CHI) are key genes in the epicatechin and anthocyanin biosynthetic pathways, respectively. CONCLUSION: Phenylpropanoid metabolites are regulated by multiple genes from the same or different families. Enzymes in different metabolic pathways compete for precursors to form a complex regulatory network for phenylalanine metabolism.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49006921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Barberry, is the main agricultural product in South Khorasan province of Iran, after saffron. Fresh fruit is perishable with short storage time. OBJECTIVE: Irradiation is an economical modern technology in food processing which maintains nutritional values and enhance shelf life of raw food without using any additives. METHODS: Barberry fruit was harvested in Birjand city by methods including “cutting branches” and “collecting fallen fruit from the cluster”. Fruit on cut branches was dried at ambient temperature under shadow which is known as “puffy barberry” locally. Fruit has fallen under shrubs was dried on a clean cloth under sunlight and locally is famed as “jewel barberry”. Some of fresh barberries were treated by osmotic solution and then they have been dried. Untreated dried fruit was processed by, freezing pasteurization and gamma irradiation at doses of 0, 3, 5 and 10 kGy. Effects of dried barberry types (puffy and jewel), processes, 6 months of storage at temperature of 4 or 25°C on texture, color and sensory properties of fruit were evaluated. RESULTS: Puffy barberry irradiated at 5 kGy by gamma radiations, after 6 months of storage at 4°C showed an acceptable quality. CONCLUSIONS: Combination of irradiation and refrigerated storage were reported as optimal conditions of processing and storage for barberry fruit.
{"title":"Effects of non-thermal processes on texture, color and sensory properties of Iranian barberry during storage","authors":"M. Ahmadi-Roshan, N. Bideli, S. B. Ardestani","doi":"10.3233/jbr-211519","DOIUrl":"https://doi.org/10.3233/jbr-211519","url":null,"abstract":"BACKGROUND: Barberry, is the main agricultural product in South Khorasan province of Iran, after saffron. Fresh fruit is perishable with short storage time. OBJECTIVE: Irradiation is an economical modern technology in food processing which maintains nutritional values and enhance shelf life of raw food without using any additives. METHODS: Barberry fruit was harvested in Birjand city by methods including “cutting branches” and “collecting fallen fruit from the cluster”. Fruit on cut branches was dried at ambient temperature under shadow which is known as “puffy barberry” locally. Fruit has fallen under shrubs was dried on a clean cloth under sunlight and locally is famed as “jewel barberry”. Some of fresh barberries were treated by osmotic solution and then they have been dried. Untreated dried fruit was processed by, freezing pasteurization and gamma irradiation at doses of 0, 3, 5 and 10 kGy. Effects of dried barberry types (puffy and jewel), processes, 6 months of storage at temperature of 4 or 25°C on texture, color and sensory properties of fruit were evaluated. RESULTS: Puffy barberry irradiated at 5 kGy by gamma radiations, after 6 months of storage at 4°C showed an acceptable quality. CONCLUSIONS: Combination of irradiation and refrigerated storage were reported as optimal conditions of processing and storage for barberry fruit.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46706498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: SQUAMOSA promoter binding protein-like (SPL) proteins are a class of plant-specific transcription factors that play important roles in plant development. However, the majority of SPL genes in strawberry are functionally uncharacterized. OBJECTIVE: To understand the biological functions and elucidate the molecular regulatory mechanisms of SPL genes in strawberry development. METHODS: The FvSPL1 gene from woodland strawberry was cloned and the phylogenetic tree was analyzed using bioinformatics methods. Subcellular localization, transcriptional activity, DNA binding ability and regulatory mechanisms of FvSPL1 were analyzed through biochemical and genetic approaches. RESULTS: Phylogenetic analysis with SPL genes from Arabidopsis, tomato and chrysanthemum indicated that FvSPL1 clustered in the same group as those of the miR156 target site located at the 3′-untranslated region. Further biochemical analysis indicated that FvSPL1 was exclusively localized in the nucleus. Electrophoretic mobility shift assay demonstrated that FvSPL1 could specifically recognize the GTAC motif. Transcriptional activity analysis showed that FvSPL1 is a transcriptional activator that could activate the expression of the FvAP1 gene. Finally, transgenic Arabidopsis overexpressing the FvSPL1 gene exhibited significantly early flowering. Taken together, our study indicated that FvSPL1, similar to its orthologs in Arabidopsis, mainly functions in regulating plant flowering. CONCLUSIONS: These findings provide insight into the mechanism of flowering in strawberry, and contribute to the understanding of strawberry flowering time manipulation which will facilitate the molecular breeding of strawberries.
{"title":"Cloning, characterization, and ectopic expression in Arabidopsis to determine the function of FvSPL1 gene from woodland strawberry (Fragaria vesca)","authors":"Yibo Bai, Moafu Liang, Z. Cheng, Jinsong Xiong","doi":"10.3233/jbr-211548","DOIUrl":"https://doi.org/10.3233/jbr-211548","url":null,"abstract":"BACKGROUND: SQUAMOSA promoter binding protein-like (SPL) proteins are a class of plant-specific transcription factors that play important roles in plant development. However, the majority of SPL genes in strawberry are functionally uncharacterized. OBJECTIVE: To understand the biological functions and elucidate the molecular regulatory mechanisms of SPL genes in strawberry development. METHODS: The FvSPL1 gene from woodland strawberry was cloned and the phylogenetic tree was analyzed using bioinformatics methods. Subcellular localization, transcriptional activity, DNA binding ability and regulatory mechanisms of FvSPL1 were analyzed through biochemical and genetic approaches. RESULTS: Phylogenetic analysis with SPL genes from Arabidopsis, tomato and chrysanthemum indicated that FvSPL1 clustered in the same group as those of the miR156 target site located at the 3′-untranslated region. Further biochemical analysis indicated that FvSPL1 was exclusively localized in the nucleus. Electrophoretic mobility shift assay demonstrated that FvSPL1 could specifically recognize the GTAC motif. Transcriptional activity analysis showed that FvSPL1 is a transcriptional activator that could activate the expression of the FvAP1 gene. Finally, transgenic Arabidopsis overexpressing the FvSPL1 gene exhibited significantly early flowering. Taken together, our study indicated that FvSPL1, similar to its orthologs in Arabidopsis, mainly functions in regulating plant flowering. CONCLUSIONS: These findings provide insight into the mechanism of flowering in strawberry, and contribute to the understanding of strawberry flowering time manipulation which will facilitate the molecular breeding of strawberries.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44266630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahra Narouei, S. Sedaghathoor, B. Kaviani, M. H. Ansari
BACKGROUND: The strawberry is an important commercial crop, the improvement of its yield and quality is an imperative task. OBJECTIVE: The present research aimed to study the effect of colored netting and foliar application of amino acids on the physiological characteristics of strawberries subjected to different irrigation intervals. METHODS: The study was carried out as a factorial experiment based on a randomized complete block design with three factors including colored net at 4 levels (no netting, green, red, and yellow netting), organic acids at 4 levels (control, humic acid, glutamine, and arginine), and three levels of irrigation intervals (2, 4, and 6 days) in the greenhouse of Lahijan Agricultural Research Station, Iran. RESULTS: The results showed that the highest leaf number, shoot weight, chlorophyll and carotenoid content were related to yellow netting. The highest fruit yield, anthocyanins, and flavonoids were observed in the treatments of no-netting, green netting, and red netting, respectively. Data for the effect of organic acids showed that the glutamine-treated plants exhibited the highest yield, the humic acid-treated plants displayed the highest anthocyanin and carotenoid content, and the arginine-treated plants demonstrated the highest vitamin C content. The irrigation interval of 6 days caused to the lowest leaf number, flower and fruit number, shoot weight, fruit yield, and carotenoid content. Data for the trilateral effect of ‘netting×organic acid×irrigation’ showed that the highest flower number and fruit yield were obtained from ‘green netting×glutamine×4 days’, the highest anthocyanin content was obtained from ‘green netting×humic acid×2 days’, and the highest chlorophyll content was obtained from ‘green netting×control×6 days’. The treatment of ‘yellow netting×control×2 days’ was related to the highest flavonoid content. CONCLUSIONS: The application of colored nets provides the strawberry with more optimal vegetative and reproductive growth.
{"title":"Effects of irrigation intervals and foliar application of amino acids and humic acid on the physiological traits of strawberries under colored shading nets","authors":"Zahra Narouei, S. Sedaghathoor, B. Kaviani, M. H. Ansari","doi":"10.3233/jbr-211516","DOIUrl":"https://doi.org/10.3233/jbr-211516","url":null,"abstract":"BACKGROUND: The strawberry is an important commercial crop, the improvement of its yield and quality is an imperative task. OBJECTIVE: The present research aimed to study the effect of colored netting and foliar application of amino acids on the physiological characteristics of strawberries subjected to different irrigation intervals. METHODS: The study was carried out as a factorial experiment based on a randomized complete block design with three factors including colored net at 4 levels (no netting, green, red, and yellow netting), organic acids at 4 levels (control, humic acid, glutamine, and arginine), and three levels of irrigation intervals (2, 4, and 6 days) in the greenhouse of Lahijan Agricultural Research Station, Iran. RESULTS: The results showed that the highest leaf number, shoot weight, chlorophyll and carotenoid content were related to yellow netting. The highest fruit yield, anthocyanins, and flavonoids were observed in the treatments of no-netting, green netting, and red netting, respectively. Data for the effect of organic acids showed that the glutamine-treated plants exhibited the highest yield, the humic acid-treated plants displayed the highest anthocyanin and carotenoid content, and the arginine-treated plants demonstrated the highest vitamin C content. The irrigation interval of 6 days caused to the lowest leaf number, flower and fruit number, shoot weight, fruit yield, and carotenoid content. Data for the trilateral effect of ‘netting×organic acid×irrigation’ showed that the highest flower number and fruit yield were obtained from ‘green netting×glutamine×4 days’, the highest anthocyanin content was obtained from ‘green netting×humic acid×2 days’, and the highest chlorophyll content was obtained from ‘green netting×control×6 days’. The treatment of ‘yellow netting×control×2 days’ was related to the highest flavonoid content. CONCLUSIONS: The application of colored nets provides the strawberry with more optimal vegetative and reproductive growth.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42986165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: Vine nutrition affects the composition of grapes, but how it impacts the aroma of grapes is largely unknown. OBJECTIVE: This work aimed to investigate the effect of different fertilizers: chemical fertilizer (CF), sheep manure-based organic fertilizer (OF), 50% organic fertilizer + 50% chemical fertilizer (O + C), 25% organic fertilizer + 25% chemical fertilizer [1/2(O + C)], and soil conditioner (SC) on the aroma accumulation of Cabernet Sauvignon grapes. METHODS: The treatments were applied and samples were collected in 2019 at weekly intervals from August 7 to September 22. The grapes’ chemical characteristics and volatile compounds were analyzed. RESULTS: The chemical results showed that the treatments had a positive effect on grapes, with a strong preference for the 1/2(O + C) treatment. Grape aroma results showed that the concentrations of grape aromas in O + C-treated samples were lower than the other treatments. The OF treated samples had comparatively high (24.8%) volatile concentrations during maturity compared to other treatments, including the control (15.9%). Throughout development, samples treated with OF (17.4%) and CF (15.7%) had higher volatile concentrations than samples treated with SC (14.4%), 1/2(O + C) (12.8%), and O + C (12.4%). However, compared to SC-treated samples, samples treated with 1/2(O + C) increased the accumulation of terpenes and esters. The principal component analysis (PCA) results showed that samples treated with OF were strongly correlated to carbonyls, terpenes, and esters during maturity. CONCLUSION: The type and ratio of fertilizer used had a significant impact on the aroma profile of Cabernet Sauvignon grapes.
{"title":"Application of different fertilizers to cabernet sauvignon vines: Effects on grape aroma accumulation","authors":"R. Salifu, Zhen Zhang, Faisal Eudes Sam, Jixin Li, Tengzhen Ma, Jing Wang, Shun-yu Han, Yu-Mei Jiang","doi":"10.3233/jbr-211517","DOIUrl":"https://doi.org/10.3233/jbr-211517","url":null,"abstract":"BACKGROUND: Vine nutrition affects the composition of grapes, but how it impacts the aroma of grapes is largely unknown. OBJECTIVE: This work aimed to investigate the effect of different fertilizers: chemical fertilizer (CF), sheep manure-based organic fertilizer (OF), 50% organic fertilizer + 50% chemical fertilizer (O + C), 25% organic fertilizer + 25% chemical fertilizer [1/2(O + C)], and soil conditioner (SC) on the aroma accumulation of Cabernet Sauvignon grapes. METHODS: The treatments were applied and samples were collected in 2019 at weekly intervals from August 7 to September 22. The grapes’ chemical characteristics and volatile compounds were analyzed. RESULTS: The chemical results showed that the treatments had a positive effect on grapes, with a strong preference for the 1/2(O + C) treatment. Grape aroma results showed that the concentrations of grape aromas in O + C-treated samples were lower than the other treatments. The OF treated samples had comparatively high (24.8%) volatile concentrations during maturity compared to other treatments, including the control (15.9%). Throughout development, samples treated with OF (17.4%) and CF (15.7%) had higher volatile concentrations than samples treated with SC (14.4%), 1/2(O + C) (12.8%), and O + C (12.4%). However, compared to SC-treated samples, samples treated with 1/2(O + C) increased the accumulation of terpenes and esters. The principal component analysis (PCA) results showed that samples treated with OF were strongly correlated to carbonyls, terpenes, and esters during maturity. CONCLUSION: The type and ratio of fertilizer used had a significant impact on the aroma profile of Cabernet Sauvignon grapes.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47301778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blueberries are one of the most popular and widely consumed berries, they are usually consumed fresh, dried or processed into juice. During the production of juices large quantities of berry press residues are generated, which are an industrial by-product and they accumulate as food waste. Optimal management of this industrial fruit by-product could help in utilising berry press residues with the purpose to valorise and add economic value. Blueberry press residues consist of skins and seeds which hold valuable compounds with antioxidant activity –polyphenolics. To facilitate the objective of bioactive compound release, the cell walls must rupture. Pectolytic enzymes could be used to degrade the structural polysaccharides of the berry skin cell wall, thus releasing the contents of the cells into the extraction medium. The physical characteristics of berry skins allows the use of pectolytic enzymes for the disruption of cells; however, the effects of enzymatic hydrolysis could be further improved using ultrasound. In this study the combined effects of enzyme and ultrasonic treatments were evaluated and optimised using the Response Surface Methodology approach in order to increase the release of polyphenolic compounds (especially anthocyanins). The optimised method was further tested to evaluate the application potential of enzyme and ultrasound treatment to prepare blueberry or lingonberry juice with increased antioxidant activity and more vibrant colour. The obtained results provide an optional method of berry press residue valorisation to produce higher quality juice or extract bioactive compounds from this type of food-waste.
{"title":"Optimisation of blueberry (Vaccinium corymbosum L.) press residue extraction using a combination of pectolytic enzyme and ultrasound treatments","authors":"L. Klavins, E.P. Puzule, J. Kviesis, M. Kļaviņš","doi":"10.3233/jbr-210722","DOIUrl":"https://doi.org/10.3233/jbr-210722","url":null,"abstract":"Blueberries are one of the most popular and widely consumed berries, they are usually consumed fresh, dried or processed into juice. During the production of juices large quantities of berry press residues are generated, which are an industrial by-product and they accumulate as food waste. Optimal management of this industrial fruit by-product could help in utilising berry press residues with the purpose to valorise and add economic value. Blueberry press residues consist of skins and seeds which hold valuable compounds with antioxidant activity –polyphenolics. To facilitate the objective of bioactive compound release, the cell walls must rupture. Pectolytic enzymes could be used to degrade the structural polysaccharides of the berry skin cell wall, thus releasing the contents of the cells into the extraction medium. The physical characteristics of berry skins allows the use of pectolytic enzymes for the disruption of cells; however, the effects of enzymatic hydrolysis could be further improved using ultrasound. In this study the combined effects of enzyme and ultrasonic treatments were evaluated and optimised using the Response Surface Methodology approach in order to increase the release of polyphenolic compounds (especially anthocyanins). The optimised method was further tested to evaluate the application potential of enzyme and ultrasound treatment to prepare blueberry or lingonberry juice with increased antioxidant activity and more vibrant colour. The obtained results provide an optional method of berry press residue valorisation to produce higher quality juice or extract bioactive compounds from this type of food-waste.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41893107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：