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Bioactive properties of vinegars produced from prunus laurocerasus L. Varieties 月桂李品种食醋的生物活性研究
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-06-19 DOI: 10.3233/jbr-230006
Kubra Yilmaz, C. Baltacı, Seyda Ozturk, Omer Karpuz
BACKGROUND: Cherry laurel fruit (CLF) has distinctive bioactive properties, which may yield new food products based on CLF. OBJECTIVE: This research aimed to realize the production of vinegar from the CLF, thus, valorizing the CLF, which is rich in antioxidants and phenolic substances, and bringing a new nutrient-rich vinegar to the market. METHODS: Seven types of traditional CLF vinegar (CLFV) from three different species were produced, and their bioactive properties were examined. The bioactivity analyses of total phenolic substance (TPC), total flavonoid (TFC), total antioxidant activity (AA), DPPHࢫ (Free radical scavenging activity), FRAP (Ferric reducing antioxidant power), ABTSࢫ + (Radical cation scavenging effect) were conducted. In addition, the composition of phenolic substances was studied. RESULTS: This study showed that CLFV performed better than controls regarding antioxidant activity, phenolic compounds, and substances. The presence of intense phenolic elements in CLFV significantly impacts its antioxidant activity. The antioxidant activities of CLFV were detected to be greater than the previous reports on bioactivities of vinegar from various fruits. CONCLUSIONS: High antioxidant and phenolic content were observed in CLFV, similar to the composition of the CLF. The usability of CLFV as a healthy and nutritious food product seems achievable on an industrial scale.
背景:樱桃月桂果(Cherry laurel fruit, CLF)具有独特的生物活性,可用于开发新型食品。目的:本研究的目的是通过将含丰富抗氧化剂和酚类物质的枸杞发酵成醋,为市场带来一种新的营养丰富的食醋。方法:采用3种不同品种生产7种传统CLFV醋,并对其生物活性进行测定。进行了总酚类物质(TPC)、总黄酮(TFC)、总抗氧化活性(AA)、DPPHࢫ(自由基清除活性)、FRAP(铁还原抗氧化能力)、ABTSࢫ +(自由基阳离子清除作用)的生物活性分析。此外,还对酚类物质的组成进行了研究。结果:本研究表明,CLFV在抗氧化活性、酚类化合物和物质方面优于对照组。CLFV中强酚类元素的存在显著影响其抗氧化活性。CLFV的抗氧化活性高于以往报道的各种水果醋的生物活性。结论:CLFV具有较高的抗氧化性和酚类成分,与CLF成分相似。CLFV作为一种健康和营养食品的可用性似乎可以在工业规模上实现。
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引用次数: 0
The effect of summer pruning time and severity on antioxidant capacity and storage quality of Hayward kiwifruit 夏季修剪时间和修剪程度对海沃德猕猴桃抗氧化能力和贮藏品质的影响
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-06-16 DOI: 10.3233/jbr-220060
Ebrahim Abedi Gheshlaghi, Massoumeh Kia Eshkvarian, M. Shiri, T. Raiesi, D. J. Mojaddad
BACKGROUND Summer pruning of kiwifruit has an important effect on fruit quality and storability. OBJECTIVE: The present study was conducted to investigate the effects of summer pruning time and severity on some quantitative and qualitative characteristics and storability of Hayward kiwifruit. METHODS: The present study was conducted to evaluate summer pruning time (one week after fruit set [1WAFS] and four weeks after fruit set [4WAFS]) and pruning severity (1 - no pruning [control], 2 - shoot pruning after the third or fourth leaf after the last fruit [common pruning], 3 - tip squeezing, and 4 - zero leaf pruning in 20% of fruit-bearing shoots with unlimited growth) on Hayward kiwifruit in 2020 and 2021. RESULTS: The results showed that, at harvest time, the highest average fruit weight and total acid were 124.00 g and 1.74% in the tip squeezing treatment observed in the 4WAFS pruning time in 2021. Zero leaf pruning produced fruits with the highest firmness and antioxidant capacity in 2020 and the highest vitamin C and total phenols in 2021. At the end of 90 days of cold storage, in the tip squeezing treatment, fruit firmness at 4WAFS pruning time was higher than in 1WAFS pruning time in the second year, and the highest antioxidant capacity was in 1WAFS pruning time in the first year. In the second year, the highest vitamin C, firmness, and dry matter were obtained in the zero leaf pruning treatment. In this treatment, the highest total phenols and antioxidant capacity were 133.96 mg GAE/100 g FW and 86.75% , respectively, observed in 4WAFS pruning time in the second year. CONCLUSIONS: Overall, tip squeezing and zero leaf pruning at the time of 4WAFS improved the quantitative and qualitative characteristics at the harvest and cold storage.
背景猕猴桃夏季修剪对果实品质和贮藏性有重要影响。目的:研究夏季修剪时间和修剪程度对海沃德猕猴桃某些数量、质量特征和贮藏性的影响。方法:本研究评估了海沃德的夏季修剪时间(坐果后一周[1WAFS]和坐果后四周[4WAFS])和修剪严重程度(1-不修剪[对照],2-在最后一个果实后第三或第四叶后修剪[普通修剪],3-顶端挤压和4-零叶修剪,对20%的无限制生长的结果枝)2020年和2021年的猕猴桃。结果:结果表明,在收获期,平均果重和总酸最高为124.00 g和1.74%。零叶修剪产生了2020年硬度和抗氧化能力最高的水果,2021年维生素C和总酚含量最高的水果。在冷藏90天结束时,在挤尖处理中,第二年4WAFS修剪时间的果实硬度高于1WAFS修剪时间,第一年1WAFS修剪时的抗氧化能力最高。在第二年,零叶修剪处理获得了最高的维生素C、硬度和干物质。在该处理中,总酚和抗氧化能力最高为133.96 mg GAE/100 g FW和86.75%。结论:总体而言,4WAFS时的顶端挤压和零叶修剪改善了收获和冷藏时的定量和定性特征。
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引用次数: 0
Influence of growing environment and pruning techniques on storage and quality traits of Vaccinium corymbosum L., cv ‘Duke’ 生长环境和修剪技术对“杜克”伞房越橘贮藏及品质性状的影响
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-06-09 DOI: 10.3233/jbr-220067
Varaldo Alice, Chiabrando Valentina, Giacalone Giovanna
BACKGROUND: Due to the increasing market demand for blueberries, the amount of land dedicated to their production is expanding. Therefore, cultivation techniques to ensure high quality and a longer shelf life have become key concerns for the blueberry industry and consumers. OBJECTIVE: This study investigated the impact of various growing environments and pruning techniques on the quality and shelf life of highbush blueberry (Vaccinium corymbosum L. cv. ‘Duke’) during cold storage. METHODS: In this study, six commercial orchards were selected based on the type of pruning (light or hard) and the plot’s altitude (lowland, hill, or mountain). After harvest, the blueberries were stored at 1°C and 90% RH for 21 days. Quality analyses were performed on days 0, 10, and 21 of storage. The data were processed with a factor-designed analysis of variance (one-way ANOVA). RESULTS: Blueberries from the lowland cultivation area with light pruning had a longer shelf life. At harvest, the lowland area had indeed produced firmer blueberries that were sweeter and less acidic, and cold storage positively preserved these characteristics compared to fruit grown at other altitudes. CONCLUSIONS: In summation, the lowland area produced firmer berries that retained their characteristics after 10 days of storage, especially those harvested from hard-pruned plants. Notably, the bioactive compounds were not affected by the pruning method. However, when combined with 10 days of storage, light pruning was associated with better preservation of the total phenol content.
背景:由于市场对蓝莓的需求不断增加,用于蓝莓生产的土地数量正在扩大。因此,确保高质量和更长保质期的栽培技术已成为蓝莓行业和消费者关注的焦点。目的:研究不同生长环境和修剪技术对高丛越橘(Vaccinium corymbolsum L.cv.‘Duke’)冷藏期间品质和保质期的影响。方法:在本研究中,根据修剪类型(轻型或硬质)和地块海拔(低地、丘陵或山地)选择了6个商业果园。收获后,将蓝莓在1°C和90%RH下储存21天。在储存的第0、10和21天进行质量分析。采用因子设计方差分析(单因素方差分析)对数据进行处理。结果:低地栽培区轻修剪蓝莓的保质期较长。在收获时,低地地区确实生产出了更结实、更甜、酸性更低的蓝莓,与其他海拔地区种植的水果相比,冷藏确实保留了这些特性。结论:总之,低地地区出产的浆果更结实,储存10天后仍保持其特性,尤其是那些从硬修剪植物中收获的浆果。值得注意的是,生物活性化合物不受修剪方法的影响。然而,当与10天的储存相结合时,轻度修剪与总酚含量的更好保存有关。
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引用次数: 0
Comparative characterization and expression analysis revealed cinnamyl alcohol dehydrogenase genes differentially responding to Colletotrichum fructicola in woodland and cultivated strawberries 比较表征和表达分析揭示了肉桂醇脱氢酶基因对林地和栽培草莓炭疽病的不同反应
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-06-09 DOI: 10.3233/jbr-230012
Zi-Yi Li, K. Duan, Jiayue Zhu, Ling Bin, Xinyi Wang, Si-Yu Chen, Xue Li, Chunnu Geng, Q. Gao
BACKGROUND: Strawberry (Fragaria×ananassa) production is hampered by anthracnose caused by Colletotrichum spp. Cinnamyl alcohol dehydrogenase (CAD) catalyzes lignin biosynthesis to fortify plant cell wall upon various stresses, but their involvement in strawberry resistance is elusive. OBJECTIVE: Current work aims to uncover the involvement of CAD genes in both woodland and cultivated strawberry responses to Colletotrichum spp. METHODS: Genome-wide identification of CAD genes was updated in cultivated strawberry. Comparative molecular characterization integrated with pathogenesis phenotyping after C. fructicola inoculation, were followed by RT-qPCR analysis and RNAseq data retrieval. RESULTS: Cultivated strawberry comprises 32 CAD genes of three classes. Thirteen syntenic relationships were observed between cultivated and woodland strawberry, with high homology in protein coding regions while promoters being greatly diversified. F. vesca ‘Hawaii4’ was least susceptible, followed by octoploid cultivar ‘ShenQi’, and ‘Benihoppe’ the most susceptible to C. fructicola. The class I CAD4 and CAD5, preferentially expressed in leaves and crowns, respectively, were up-regulated in susceptible varieties at 72 h post inoculation (hpi). Of class II, CAD8 was up-regulated in three genotypes at distinct timepoints, while CAD12 was quickly down-regulated in ‘ShenQi’ upon inoculation. CAD1 and CAD2 of class III were down-regulated in ‘ShenQi’ and ‘Hawaii4’ at distinct stages. The strong involvement of CAD genes in strawberry response to Colletotrichum spp. sets a basis for future functional study. CONCLUSION: F. vesca is less susceptible to Colletotrichum spp. than cultivated strawberries but the expression of CAD family in F. vesca and F.×ananassa were differentially provoked in timing and magnitude hinting no correlation with strawberry resistance to this pathogen.
背景:炭疽菌引起的炭疽病阻碍了草莓(Fragaria×ananassa)的生产。肉桂醇脱氢酶(CAD)在各种胁迫下催化木质素生物合成以增强植物细胞壁,但它们与草莓抗性的关系尚不清楚。目的:目前的工作旨在揭示CAD基因在林地和栽培草莓对炭疽菌的反应中的作用。方法:更新栽培草莓CAD基因的全基因组鉴定。将比较分子特征与接种后的发病机制表型相结合,然后进行RT-qPCR分析和RNAseq数据检索。结果:栽培草莓含有3类32个CAD基因。在栽培草莓和林地草莓之间观察到13个同基因关系,在蛋白质编码区具有高度同源性,而启动子则非常多样化。黄颡鱼‘Hawaii4’最不易感,其次是八倍体品种‘ShenQi’,而‘Benihope’最易感。I类CAD4和CAD5分别优先在叶片和树冠中表达,在72岁时在易感品种中上调 接种后h(hpi)。在II类中,CAD8在三种基因型中在不同的时间点上调,而CAD12在“神芪”中在接种后迅速下调。Ⅲ类CAD1和CAD2在‘申启’和‘夏威夷’不同阶段均下调。CAD基因在草莓对炭疽菌的反应中的强烈参与为未来的功能研究奠定了基础。结论:与栽培草莓相比,vesca对Colletotrichum spp.的敏感性较低,但CAD家族在vesca和×ananassa中的表达在时间和数量上存在差异,这与草莓对该病原体的抗性无关。
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引用次数: 0
A powdered mix of cape gooseberry-based pulp, seed, and peel: Optimization of formulation and spray-drying process 以醋栗为原料的果肉、种子和果皮的粉末状混合物:配方和喷雾干燥工艺的优化
4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-06-07 DOI: 10.3233/jbr-220079
Soany Eraso-Grisales, Misael Cortés-Rodríguez, Manuela Gallón-Bedoya, Edgar Herrera-Herrera, Rodrigo Ortega-Toro
BACKGROUND: Cape gooseberry (CG) is a fruit with nutritional and sensory attributes that generate significant consumer interest. Consumption patterns of this fruit must be in line with the diversification of its value-added products to be offered to new markets. OBJECTIVE: The research aim was to optimize the encapsulants formulation and the spray-drying (SD) process for developing of a powdered mix (PMCG) of CG based pulp, seed and peel, using the response surface methodology (RSM). METHODS: A RSM was used, with a face-centered central composite experimental design (α= 1). The independent variables (IV) were gum arabic (GA) (1 – 3% w/w), maltodextrin (MD) (11,5 – 13,5% w/w), air inlet temperature (AIT) (130 – 160 °C), air outlet temperature (AOT) (75 – 85 °C), and atomizer disc speed (ADS) (18000 – 22000 rpm). The dependent variables (DV) of the PMCG were moisture, solubility, hygroscopicity, wettability (We), color coordinates L* and b*, total phenols (TP), antioxidant capacity, and yield. RESULTS: The experimental optimization presented a desirability of 68.0%, with GA and MD levels in the PMCG of 2.2 and 10.1% p/p, respectively, and process conditions of AIT = 160 °C, AOT = 77.8 °C and ADS = 21450 rpm. The IV with the most significant effect on the DV was the MD. It was observed that the increase in MD decreases the PMCG moisture content, hygroscopicity, and the content of bioactive compounds, but increases the solubility and the values of L* and b*. The SD process was an effective technology that allowed obtaining PMCG with excellent quality attributes. CONCLUSIONS: In the process, greater use was made of the structure of the CG (pulp, seed, and peel), which gave high CG solids to the PMCG obtained and allowed more effective use of the fruit and higher economic viability in the SD process.
背景:角醋栗(CG)是一种具有营养和感官属性的水果,引起了消费者的极大兴趣。这种水果的消费模式必须与其提供给新市场的增值产品的多样化相一致。目的:利用响应面法(RSM)优化包封剂配方和喷雾干燥工艺,以制备CG基纸浆、种子和果皮粉末混合物。方法:采用面心中心复合实验设计(α= 1)的RSM,自变量为阿拉伯胶(GA) (1 ~ 3% w/w)、麦芽糊精(MD) (11,5 ~ 13,5% w/w)、进风口温度(AIT)(130 ~ 160℃)、出风口温度(AOT)(75 ~ 85℃)、雾滴盘转速(ADS) (18000 ~ 22000 rpm)。PMCG的因变量为水分、溶解度、吸湿性、润湿性(We)、颜色坐标L*和b*、总酚(TP)、抗氧化能力和产率。结果:优化后的工艺条件为:AIT = 160℃,AOT = 77.8℃,ADS = 21450 rpm, PMCG中GA和MD含量分别为2.2 p/p和10.1% p/p。对DV影响最显著的IV是MD, MD的增加降低了PMCG的含水率、吸湿性和生物活性化合物的含量,但增加了溶解度和L*、b*的值。SD工艺是一种有效的技术,可以获得具有优良质量属性的PMCG。结论:在该工艺中,更多地利用了CG(果肉、种子和果皮)的结构,使所获得的PMCG具有较高的CG固含量,使果实得到更有效的利用,并在SD工艺中具有更高的经济可行性。
{"title":"A powdered mix of cape gooseberry-based pulp, seed, and peel: Optimization of formulation and spray-drying process","authors":"Soany Eraso-Grisales, Misael Cortés-Rodríguez, Manuela Gallón-Bedoya, Edgar Herrera-Herrera, Rodrigo Ortega-Toro","doi":"10.3233/jbr-220079","DOIUrl":"https://doi.org/10.3233/jbr-220079","url":null,"abstract":"BACKGROUND: Cape gooseberry (CG) is a fruit with nutritional and sensory attributes that generate significant consumer interest. Consumption patterns of this fruit must be in line with the diversification of its value-added products to be offered to new markets. OBJECTIVE: The research aim was to optimize the encapsulants formulation and the spray-drying (SD) process for developing of a powdered mix (PMCG) of CG based pulp, seed and peel, using the response surface methodology (RSM). METHODS: A RSM was used, with a face-centered central composite experimental design (α= 1). The independent variables (IV) were gum arabic (GA) (1 – 3% w/w), maltodextrin (MD) (11,5 – 13,5% w/w), air inlet temperature (AIT) (130 – 160 °C), air outlet temperature (AOT) (75 – 85 °C), and atomizer disc speed (ADS) (18000 – 22000 rpm). The dependent variables (DV) of the PMCG were moisture, solubility, hygroscopicity, wettability (We), color coordinates L* and b*, total phenols (TP), antioxidant capacity, and yield. RESULTS: The experimental optimization presented a desirability of 68.0%, with GA and MD levels in the PMCG of 2.2 and 10.1% p/p, respectively, and process conditions of AIT = 160 °C, AOT = 77.8 °C and ADS = 21450 rpm. The IV with the most significant effect on the DV was the MD. It was observed that the increase in MD decreases the PMCG moisture content, hygroscopicity, and the content of bioactive compounds, but increases the solubility and the values of L* and b*. The SD process was an effective technology that allowed obtaining PMCG with excellent quality attributes. CONCLUSIONS: In the process, greater use was made of the structure of the CG (pulp, seed, and peel), which gave high CG solids to the PMCG obtained and allowed more effective use of the fruit and higher economic viability in the SD process.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135494062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of drying kinetics and quality parameters of rosehip pestils dried by three different methods 三种不同干燥方法对玫瑰果干燥动力学及品质参数的评价
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-05-19 DOI: 10.3233/jbr-230013
A. Ö. Karabacak
BACKGROUND: Rosehip fruits cannot be eaten as entire fruits in their fresh feature so they processed into various products including pestils. OBJECTIVE: In the current work, rosehip pestil as a new healthy snack food was developed by using hot air (HA) drying at 55, 65 and 75°C, vacuum (VC) drying at 55, 65 and 75°C with 25 kPa and microwave (MCW) drying at 90 and 180 W. METHODS: The effect of three different drying methods on drying kinetics and quality parameters i.e., total phenolic content (TPC), antioxidant capacity (AC), Hydroxymethylfurfural (HMF) formation, lycopene, β-carotene content, element composition, color, texture and sensorial properties of rosehip pestils was studied. RESULTS: Wang and Singh model was best fitted for MCW drying at 90 W, however Page and Modified Page models were best fitted for other drying conditions. The rosehip pestils dried by MCW drying method had highest drying rate, TPC, AC, lycopene and β-carotene content, element concentration, color values, whereas their HMF content and sensorial properties were not acceptable. Sensory evaluation results indicated that rosehip pestils dried with HA was rated the most acceptable. CONCLUSIONS: It was concluded that rosehip pestil with its sensorial and nutritive properties could be an alternative product for the functional food market. However, it has been seen that the drying methods used alone have some disadvantages. For this reason, it may be possible to eliminate these disadvantages if hybrid drying methods (for example MCW drying followed by HA or VC drying) are preferred in future studies.
背景:玫瑰果的新鲜特性不能作为完整的水果食用,因此它们被加工成包括鼠疫在内的各种产品。目的:采用55、65和75°C的热风干燥,55、65、75°C 25kPa的真空干燥,90和180的微波干燥,研制出一种新的健康休闲食品——玫瑰果鼠疫菌 W.方法:研究三种不同干燥方法对玫瑰果鼠疫菌的干燥动力学和质量参数,即总酚含量(TPC)、抗氧化能力(AC)、羟甲基糠醛(HMF)形成、番茄红素、β-胡萝卜素含量、元素组成、颜色、质地和感官特性的影响。结果:王和辛格模型最适合MCW 90干燥 W、 然而Page和Modified Page模型最适合其他干燥条件。用MCW干燥法干燥的玫瑰果鼠疫菌的干燥速率、TPC、AC、番茄红素和β-胡萝卜素含量、元素浓度、颜色值最高,但其HMF含量和感官特性不合格。感官评价结果表明,用HA干燥的玫瑰果鼠疫菌被评为最可接受的。结论:鼠疫玫瑰果具有良好的感官和营养特性,可作为功能性食品市场的替代品。然而,已经发现单独使用的干燥方法有一些缺点。因此,如果在未来的研究中优选混合干燥方法(例如MCW干燥,然后是HA或VC干燥),则可能消除这些缺点。
{"title":"Evaluation of drying kinetics and quality parameters of rosehip pestils dried by three different methods","authors":"A. Ö. Karabacak","doi":"10.3233/jbr-230013","DOIUrl":"https://doi.org/10.3233/jbr-230013","url":null,"abstract":"BACKGROUND: Rosehip fruits cannot be eaten as entire fruits in their fresh feature so they processed into various products including pestils. OBJECTIVE: In the current work, rosehip pestil as a new healthy snack food was developed by using hot air (HA) drying at 55, 65 and 75°C, vacuum (VC) drying at 55, 65 and 75°C with 25 kPa and microwave (MCW) drying at 90 and 180 W. METHODS: The effect of three different drying methods on drying kinetics and quality parameters i.e., total phenolic content (TPC), antioxidant capacity (AC), Hydroxymethylfurfural (HMF) formation, lycopene, β-carotene content, element composition, color, texture and sensorial properties of rosehip pestils was studied. RESULTS: Wang and Singh model was best fitted for MCW drying at 90 W, however Page and Modified Page models were best fitted for other drying conditions. The rosehip pestils dried by MCW drying method had highest drying rate, TPC, AC, lycopene and β-carotene content, element concentration, color values, whereas their HMF content and sensorial properties were not acceptable. Sensory evaluation results indicated that rosehip pestils dried with HA was rated the most acceptable. CONCLUSIONS: It was concluded that rosehip pestil with its sensorial and nutritive properties could be an alternative product for the functional food market. However, it has been seen that the drying methods used alone have some disadvantages. For this reason, it may be possible to eliminate these disadvantages if hybrid drying methods (for example MCW drying followed by HA or VC drying) are preferred in future studies.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43802401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hybrid RSM-GA approach to optimize extraction conditions for blueberry anthocyanins with high antioxidant activity 杂交RSM-GA法优化高抗氧化活性蓝莓花青素提取工艺
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-04-28 DOI: 10.3233/jbr-230016
X. Fang, Weijie Wu, Honglei Mu, Hangjun Chen, Xiaolin Zheng, Haiyan Gao
BACKGROUND: Blueberry contains an abundance of anthocyanins, which are a bioactive component of this fruit. Anthocyanins can be extracted via various methods, and each has pros and cons. OBJECTIVE: This current study reported the optimal conditions for the ultrasonic-assisted enzymatic extraction of blueberry anthocyanins simulated using response surface methodology (RSM) coupled with a genetic algorithm (GA). METHODS: The Box–Behnken design (BBD) was used for the RSM, and the extraction conditions were as follows: temperature, 42°C; ultrasonic power, 310 W; enzyme volume, 0.25% ; and extraction time, 42 min. RESULTS: The maximum predicted extraction yield was 6.67 mg/g. The antioxidant activity of anthocyanins extracted via RSM and GA was based on the hydroxyl free radical activity and supersonic anion free radical activity of 230.50±12.76μg/ml and 4.41±0.36μg/ml, respectively. Anthocyanins exracted by the proposed method has stronger free radical removal capacity than that of Vc. CONCLUSIONS: This study shows that the combination of RSM with GA represents an optimized method for extracting blueberry anthocyanins for use in the food industry. This method can maintain high antioxidant potential and can be used as an alternative strategy for high-value products.
背景:蓝莓含有丰富的花青素,这是这种水果的生物活性成分。花青素的提取方法多种多样,各有优缺点。目的:采用响应面法(RSM)结合遗传算法(GA)模拟超声辅助酶法提取蓝莓花青素的最佳工艺条件。方法:采用Box-Behnken设计(BBD),提取条件为:温度42℃;超声波功率310 W;酶体积,0.25%;提取时间为42 min。结果:预测最大提取量为6.67 mg/g。RSM法和GA法提取的花青素抗氧化活性分别为:羟基自由基活性为230.50±12.76μg/ml,超音速阴离子自由基活性为4.41±0.36μg/ml。该方法提取的花青素具有比Vc更强的自由基清除能力。结论:本研究表明,RSM - GA联合提取蓝莓花青素是一种可用于食品工业的优化方法。该方法可保持较高的抗氧化潜力,可作为高价值产品的替代策略。
{"title":"Hybrid RSM-GA approach to optimize extraction conditions for blueberry anthocyanins with high antioxidant activity","authors":"X. Fang, Weijie Wu, Honglei Mu, Hangjun Chen, Xiaolin Zheng, Haiyan Gao","doi":"10.3233/jbr-230016","DOIUrl":"https://doi.org/10.3233/jbr-230016","url":null,"abstract":"BACKGROUND: Blueberry contains an abundance of anthocyanins, which are a bioactive component of this fruit. Anthocyanins can be extracted via various methods, and each has pros and cons. OBJECTIVE: This current study reported the optimal conditions for the ultrasonic-assisted enzymatic extraction of blueberry anthocyanins simulated using response surface methodology (RSM) coupled with a genetic algorithm (GA). METHODS: The Box–Behnken design (BBD) was used for the RSM, and the extraction conditions were as follows: temperature, 42°C; ultrasonic power, 310 W; enzyme volume, 0.25% ; and extraction time, 42 min. RESULTS: The maximum predicted extraction yield was 6.67 mg/g. The antioxidant activity of anthocyanins extracted via RSM and GA was based on the hydroxyl free radical activity and supersonic anion free radical activity of 230.50±12.76μg/ml and 4.41±0.36μg/ml, respectively. Anthocyanins exracted by the proposed method has stronger free radical removal capacity than that of Vc. CONCLUSIONS: This study shows that the combination of RSM with GA represents an optimized method for extracting blueberry anthocyanins for use in the food industry. This method can maintain high antioxidant potential and can be used as an alternative strategy for high-value products.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46178396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive evaluation of nine grape varieties based on fundamental physical and chemical indicators, color and volatile compounds 基于基本理化指标、色泽和挥发性物质的9个葡萄品种综合评价
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-04-28 DOI: 10.3233/jbr-220080
W. Cao, Nan Shu, Yiming Yang, Jinli Wen, Wenpeng Lu
BACKGROUND: In todays’ society, the rapid development of the wine industry and the improvement of peoples’ living standards make people pay more and more attention to wine grape quality. OBJECTIVE: To evaluate the wine grape varieties in Northeast Chinas’ grape growing regions for better wine grape quality, we evaluated the quality of different varieties of wine grapes. METHODS: The grape varieties ‘Hassan’ ‘Zuoshaner’ ‘Beibinghong’ ‘Zuoyouhong’ ‘Beta’ ‘Shuanghong’ ‘Zijingganlu’ ‘Cabernet Sauvignon’ and ‘Syrah’ were planted in the grape growing area of Jilin, Northeast China, were used as the subjects of this study. The grape berries were analyzed and tested for morphological indicators, basic physicochemical indicators, color, and phenolic and aromatic composition. RESULTS: According to lab results, ‘Hassan’ contained the highest amount of total phenolics; ‘Zuoyouhong’ had the highest solids and total sugar content; ‘Shuanghong’ had the most elevated total acid and anthocyanin content; ‘Zijngganlu’ had the highest tannin content and acid fixation ratio; Seventy-one volatile compounds were detected in nine grape varieties. CONCLUSIONS: Each of the nine grape varieties has a distinctive flavor, and because of this, grape processing products with regional flavors can be created. The same offer valuable data for future scientific grape resource collection, conservation, and exploitation.
背景:在当今社会,葡萄酒工业的快速发展和人们生活水平的提高,使人们越来越关注葡萄酒的质量。目的:为了评价东北葡萄产区的酿酒葡萄品种以获得更好的酿酒葡萄质量,我们对不同品种的酿酒葡萄进行了质量评价。方法:以东北吉林葡萄产区的哈桑、左山儿、北冰红、左右红、贝塔、双红、紫荆甘露、赤霞珠和西拉葡萄品种为研究对象。对葡萄浆果的形态指标、基本理化指标、颜色、酚类和芳香成分进行了分析和测试。结果:根据实验室结果,‘哈桑’的总酚含量最高左右红的固体含量和总糖含量最高双红的总酸和花青素含量最高紫荆甘露的单宁含量和固酸率最高;在9个葡萄品种中检测到71种挥发性化合物。结论:九个葡萄品种中的每一个都有独特的风味,因此可以创造出具有地区风味的葡萄加工产品。这为未来科学的葡萄资源收集、保护和开发提供了宝贵的数据。
{"title":"Comprehensive evaluation of nine grape varieties based on fundamental physical and chemical indicators, color and volatile compounds","authors":"W. Cao, Nan Shu, Yiming Yang, Jinli Wen, Wenpeng Lu","doi":"10.3233/jbr-220080","DOIUrl":"https://doi.org/10.3233/jbr-220080","url":null,"abstract":"BACKGROUND: In todays’ society, the rapid development of the wine industry and the improvement of peoples’ living standards make people pay more and more attention to wine grape quality. OBJECTIVE: To evaluate the wine grape varieties in Northeast Chinas’ grape growing regions for better wine grape quality, we evaluated the quality of different varieties of wine grapes. METHODS: The grape varieties ‘Hassan’ ‘Zuoshaner’ ‘Beibinghong’ ‘Zuoyouhong’ ‘Beta’ ‘Shuanghong’ ‘Zijingganlu’ ‘Cabernet Sauvignon’ and ‘Syrah’ were planted in the grape growing area of Jilin, Northeast China, were used as the subjects of this study. The grape berries were analyzed and tested for morphological indicators, basic physicochemical indicators, color, and phenolic and aromatic composition. RESULTS: According to lab results, ‘Hassan’ contained the highest amount of total phenolics; ‘Zuoyouhong’ had the highest solids and total sugar content; ‘Shuanghong’ had the most elevated total acid and anthocyanin content; ‘Zijngganlu’ had the highest tannin content and acid fixation ratio; Seventy-one volatile compounds were detected in nine grape varieties. CONCLUSIONS: Each of the nine grape varieties has a distinctive flavor, and because of this, grape processing products with regional flavors can be created. The same offer valuable data for future scientific grape resource collection, conservation, and exploitation.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47879399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Optimization of microwave assisted extraction and biological activities of total flavonoids from grape seed using response surface methodology 响应面法优化微波辅助提取葡萄籽总黄酮及其生物活性
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-04-01 DOI: 10.3233/jbr-220081
Yao Wen, Caimei Su, Xueying Mai
Aiming to reduce the environmental contamination and resource wastage generated by discarded grape seed, the management of discarded grape seed should be carried out and their potential bioactivities and pharmacological properties ought to be exploited to the fullest extent possible. A Box–Behnken design of response surface methodology was employed to further optimize microwave-assisted extraction setting for grape seed total flavonoids (GSFE). In addition, the extract (GSFE) of total flavonoids obtained were refined through AB-8 macroporous resin for obtaining more pure flavonoid (GSFP) with determination of their in vitro hypoglycemic activity and antioxidant activity. The results indicated that following optimized extraction conditions were achieved: ethanol concentration 50% liquid-solid ratio 21:1 mL/g, microwave power 540 W, microwave time 126 s. Below mentioned conditions, the extraction yield reached an average experimental value (1.979 mg/g), representing 1.69 times higher than that of water extraction (1.17 mg/g). In contrast to GSFE, the purity of GSFE after purification (GSFP) exhibited a high degree of purity at 13.753 mg/g, representing nearly 2.50 times that of GSFE (3.926 mg/g). GSFP possessed high α-glucosidase and α-amylase inhibitory activities as well as DPPH and ABTS + free radical scavenging activities with an IC50 value of 0.00641, 0.0257, 0.0669 and 0.0708 mg/mL, inferior to that of GSFE (0.0514, 0.186, 0.129 and 0.223 mg/g). Within present work, the microwave technique for extraction of total flavonoids from grape seeds was investigated with an efficient and cost-effective extraction method. Total flavonoids of grape seeds have certain in vitro blood glucose and antioxidant activity, and the purified total flavonoid biological activity has improved, laying the foundation for further study of its pharmacological activity.
为了减少废弃葡萄籽对环境的污染和资源的浪费,应对废弃葡萄籽进行管理,并尽可能充分利用其潜在的生物活性和药理特性。采用响应面的Box-Behnken设计方法进一步优化了葡萄籽总黄酮(GSFE)的微波辅助提取条件。此外,通过AB-8大孔树脂对所获得的总黄酮提取物(GSFE)进行精制,以获得更纯的黄酮类化合物(GSFP),并测定其体外降血糖活性和抗氧化活性。结果表明,最佳提取条件为:乙醇浓度50%液固比21:1 mL/g,微波功率540 W、 微波时间126 在上述条件下,提取产率达到平均实验值(1.979 mg/g),是水提取(1.17)的1.69倍 mg/g)。与GSFE相比,纯化后的GSFE的纯度(GSFP)显示出13.753的高纯度 mg/g,几乎是GSFE的2.50倍(3.926 mg/g)。GSFP具有较高的α-葡萄糖苷酶和α-淀粉酶抑制活性以及DPPH和ABTS+自由基清除活性,IC50值分别为0.00641、0.0257、0.0669和0.0708 mg/mL,低于GSFE(0.0514、0.186、0.129和0.223 mg/g)。在本工作中,研究了微波技术提取葡萄籽中总黄酮的方法,该方法是一种高效、经济的提取方法。葡萄籽总黄酮具有一定的体外血糖和抗氧化活性,纯化后的总黄酮生物活性有所提高,为进一步研究其药理活性奠定了基础。
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引用次数: 0
Evaluation of yield, biochemical characteristics and nutrient composition of ‘Camarosa’ strawberry in response to different K/N ratios 不同钾氮比对“Camarosa”草莓产量、生化特性及营养成分的影响
IF 1.7 4区 农林科学 Q3 PLANT SCIENCES Pub Date : 2023-03-15 DOI: 10.3233/jbr-220056
S. Yousefi, S. Eshghi, B. Jamali
BACKGROUND: The normal growth and yield of strawberry plant are affected by lack of balanced fertilization. OBJECTIVE: This study was carried out to evaluate the effects of different N/K ratios of nutrient solution on yield, some biochemical characteristics, macro-, and micronutrient concentrations, absorption, and translocation factor in strawberry ‘Camarosa’. METHODS: Three concentration levels of K (200, 250, and 300 mg l–1, using KNO3, KCl, and K2SO4) and two concentration levels of N (120 and 180 mg l–1, using NH4H2PO4, Ca(NO3)2, and KNO3) were applied during two vegetative and reproductive growth stages. RESULTS: The results showed that the highest productivity (149.05 g/plant), soluble sugars, and starch content were obtained from plants which had received a K/N ratio of 200/180 and 300/120 mg l–1 during the vegetative and reproductive stages, respectively. Plants treated with the highest K and N concentrations (300 and 180 mg l–1, respectively) during the vegetative and reproductive stages had the highest leaf area, photochemical efficiency of photosystem II (Fv/Fm), as well as shoot and root dry weight. High N and low K concentration in the nutrient solution increased concentrations of N, Ca, Mg, Mn, and Zn while decreased K and Fe concentrations. In addition, higher N levels increased the uptake of N, P, K, Ca, Mg, Mn, and Zn. At a K/N ratio of 200/120 mg l–1, the translocation factor of N decreased, while the translocation factor of K and Mg increased. CONCLUSION: In conclusion, the changes in the K/N ratio significantly affected the concentration of nutrient elements, absorption and translocation factor.
背景:草莓植株的正常生长和产量受到缺乏平衡施肥的影响。目的:研究不同氮钾比营养液对卡马罗萨草莓产量、某些生化特性、常量和微量营养素浓度、吸收和转运因子的影响。方法:三种浓度水平的K(200、250和300 mg l–1,使用KNO3、KCl和K2SO4)和两种浓度水平的N(120和180 mg l–1,使用NH4H2PO4、Ca(NO3)2和KNO3)在两个营养生长和生殖生长阶段施用。结果:结果表明,生产效率最高(149.05 g/植物)、可溶性糖和淀粉含量从K/N比为200/180和300/120的植物中获得 在营养期和生殖期分别为mg l–1。用最高K和N浓度处理的植物(300和180 mg l–1)在营养和生殖阶段具有最高的叶面积、光系统II的光化学效率(Fv/Fm)以及地上部和根部干重。营养液中的高N和低K浓度增加了N、Ca、Mg、Mn和Zn的浓度,而降低了K和Fe的浓度。此外,较高的氮水平增加了对N、P、K、Ca、Mg、Mn和Zn的吸收。K/N比为200/120时 mg l–1时,N的易位因子降低,而K和mg的易位因子增加。结论:总的来说,K/N比值的变化显著影响营养元素的浓度、吸收和转运因子。
{"title":"Evaluation of yield, biochemical characteristics and nutrient composition of ‘Camarosa’ strawberry in response to different K/N ratios","authors":"S. Yousefi, S. Eshghi, B. Jamali","doi":"10.3233/jbr-220056","DOIUrl":"https://doi.org/10.3233/jbr-220056","url":null,"abstract":"BACKGROUND: The normal growth and yield of strawberry plant are affected by lack of balanced fertilization. OBJECTIVE: This study was carried out to evaluate the effects of different N/K ratios of nutrient solution on yield, some biochemical characteristics, macro-, and micronutrient concentrations, absorption, and translocation factor in strawberry ‘Camarosa’. METHODS: Three concentration levels of K (200, 250, and 300 mg l–1, using KNO3, KCl, and K2SO4) and two concentration levels of N (120 and 180 mg l–1, using NH4H2PO4, Ca(NO3)2, and KNO3) were applied during two vegetative and reproductive growth stages. RESULTS: The results showed that the highest productivity (149.05 g/plant), soluble sugars, and starch content were obtained from plants which had received a K/N ratio of 200/180 and 300/120 mg l–1 during the vegetative and reproductive stages, respectively. Plants treated with the highest K and N concentrations (300 and 180 mg l–1, respectively) during the vegetative and reproductive stages had the highest leaf area, photochemical efficiency of photosystem II (Fv/Fm), as well as shoot and root dry weight. High N and low K concentration in the nutrient solution increased concentrations of N, Ca, Mg, Mn, and Zn while decreased K and Fe concentrations. In addition, higher N levels increased the uptake of N, P, K, Ca, Mg, Mn, and Zn. At a K/N ratio of 200/120 mg l–1, the translocation factor of N decreased, while the translocation factor of K and Mg increased. CONCLUSION: In conclusion, the changes in the K/N ratio significantly affected the concentration of nutrient elements, absorption and translocation factor.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49209603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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Journal of Berry Research
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