Hader-Iván Castaño-Peláez, Misael Cortes Rodríguez, J. Gil G, Gloria L. López, R. Ortega-Toro
BACKGROUND: The strawberry is a fruit with great acceptance by the consumer due to its pleasant flavor and functionality; It is rich in vitamin C, tannins, flavonoids, anthocyanins, catechin, quercetin and kaempferol, organic acids and minerals. OBJETIVE: The objective of the research was to evaluate the effect of the feed formulation and the spray-drying process on the quality of strawberry extract microcapsules (EMs). METHODS: The response surface methodology was used with a central composite design centered on the face (α= 1), considering the factors: maltodextrin (MD), inlet air temperature (IAT), outlet air temperature (OAT) and atomizing disk speed (ADS), and the response variables: humidity (Xw), water activity (aw), solubility (S), wettability ( We ), hygroscopicity (Hy), bulk density (ρ b), compacted density (ρ c), total phenols (TP), antioxidant capacity, angle of repose (AR), strawberry powder particle size (D [3 ;2] ), color (CIE-Lab), and process yield (Y). RESULTS: The aw and Xw values guarantee microbiological stability and control over degradation reactions in EMs. For the levels evaluated, the S was affected by OAT and ADS, and interaction IAT*MD, ADS*MD, IAT2, and MD2. The We depended on the factors IAT, OAT, and the interactions IAT*OAT and IAT*MD. ABTS activity was affected by the factors ADS, ADS*MD and IAT2. The Y was affected by OAT, MD, IAT*OAT, IOT*ADS, IOT*MD, OAT*ADS, ADS*MD, IOT2, and OAT2. The increase in MD caused a decrease in Hy. The value of L* was affected by ADS and MD, effects due to temperature did not affect the levels evaluated, and chromaticity a* was positively affected by the increase in ADS. IAT and MD presented a statistical effect on AR. CONCLUSIONS: The experimental optimization reached a desirability of 73.7%, being the optimal conditions: IAT (154°C), OAT (89°C), ADS (16,805 rpm), and MD (11.5%).
{"title":"Optimization of spray-drying process parameters on strawberry (Fragaria ananassa D.) extracts microcapsules quality","authors":"Hader-Iván Castaño-Peláez, Misael Cortes Rodríguez, J. Gil G, Gloria L. López, R. Ortega-Toro","doi":"10.3233/jbr-220047","DOIUrl":"https://doi.org/10.3233/jbr-220047","url":null,"abstract":"BACKGROUND: The strawberry is a fruit with great acceptance by the consumer due to its pleasant flavor and functionality; It is rich in vitamin C, tannins, flavonoids, anthocyanins, catechin, quercetin and kaempferol, organic acids and minerals. OBJETIVE: The objective of the research was to evaluate the effect of the feed formulation and the spray-drying process on the quality of strawberry extract microcapsules (EMs). METHODS: The response surface methodology was used with a central composite design centered on the face (α= 1), considering the factors: maltodextrin (MD), inlet air temperature (IAT), outlet air temperature (OAT) and atomizing disk speed (ADS), and the response variables: humidity (Xw), water activity (aw), solubility (S), wettability ( We ), hygroscopicity (Hy), bulk density (ρ b), compacted density (ρ c), total phenols (TP), antioxidant capacity, angle of repose (AR), strawberry powder particle size (D [3 ;2] ), color (CIE-Lab), and process yield (Y). RESULTS: The aw and Xw values guarantee microbiological stability and control over degradation reactions in EMs. For the levels evaluated, the S was affected by OAT and ADS, and interaction IAT*MD, ADS*MD, IAT2, and MD2. The We depended on the factors IAT, OAT, and the interactions IAT*OAT and IAT*MD. ABTS activity was affected by the factors ADS, ADS*MD and IAT2. The Y was affected by OAT, MD, IAT*OAT, IOT*ADS, IOT*MD, OAT*ADS, ADS*MD, IOT2, and OAT2. The increase in MD caused a decrease in Hy. The value of L* was affected by ADS and MD, effects due to temperature did not affect the levels evaluated, and chromaticity a* was positively affected by the increase in ADS. IAT and MD presented a statistical effect on AR. CONCLUSIONS: The experimental optimization reached a desirability of 73.7%, being the optimal conditions: IAT (154°C), OAT (89°C), ADS (16,805 rpm), and MD (11.5%).","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42117932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hader Castaño, Misael Cortés Rodríguez, Jesús Gil, Gloria L. López, R. Ortega-Toro
BACKGROUND: The microencapsulation process using spray drying (SD) represents an effective alternative in protecting the active components present in strawberries. However, microcapsules of strawberry powder mixtures present problems of instantanisation and flowability; an aspect that can be solved by agglomeration of the particles. OBJECTIVE: The aim of this study was to evaluate the influence of the fluidised bed agglomeration process on the flow, instantaneity and antioxidant properties of strawberry powder obtained by SD. METHODS: The response surface methodology (RSM) was used with a centred composite central design (α=1), considering the factors: fluidisation air temperature (50-70°C), time (30-50 min) and atomisation air pressure of the binder agent (1-2 bar). RESULTS: An increase in particle size was observed in the agglomeration process; the agglomerated particles showed a decrease in wetting time, the agglomerates of strawberry powder mixtures presented excellent instantanisation and fluidity, solving the problems identified in the microcapsules obtained by SD. CONCLUSIONS: Although the moisture and aw levels were increased in the agglomeration process, the values are within the microbiological and physicochemical food safety range; moreover, there was no effect on the phenol content and antioxidant capacity. The experimental optimisation achieved desirability of 68.4%, the optimum conditions being 70°C, 30 min and 1 bar.
{"title":"Fluidised bed agglomeration of strawberry powder mix obtained for spray drying","authors":"Hader Castaño, Misael Cortés Rodríguez, Jesús Gil, Gloria L. López, R. Ortega-Toro","doi":"10.3233/jbr-220036","DOIUrl":"https://doi.org/10.3233/jbr-220036","url":null,"abstract":"BACKGROUND: The microencapsulation process using spray drying (SD) represents an effective alternative in protecting the active components present in strawberries. However, microcapsules of strawberry powder mixtures present problems of instantanisation and flowability; an aspect that can be solved by agglomeration of the particles. OBJECTIVE: The aim of this study was to evaluate the influence of the fluidised bed agglomeration process on the flow, instantaneity and antioxidant properties of strawberry powder obtained by SD. METHODS: The response surface methodology (RSM) was used with a centred composite central design (α=1), considering the factors: fluidisation air temperature (50-70°C), time (30-50 min) and atomisation air pressure of the binder agent (1-2 bar). RESULTS: An increase in particle size was observed in the agglomeration process; the agglomerated particles showed a decrease in wetting time, the agglomerates of strawberry powder mixtures presented excellent instantanisation and fluidity, solving the problems identified in the microcapsules obtained by SD. CONCLUSIONS: Although the moisture and aw levels were increased in the agglomeration process, the values are within the microbiological and physicochemical food safety range; moreover, there was no effect on the phenol content and antioxidant capacity. The experimental optimisation achieved desirability of 68.4%, the optimum conditions being 70°C, 30 min and 1 bar.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45865656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raju Mondal, Sreya Antony, M. C. Thriveni, G. Thanavendan, G. Ravikumar, B. Sreenivasa
BACKGROUND: Morin, (3,5,7,2′,4′-pentahydroxyflavone), is a polyphenolic compound belonging to bio-flavonoids and is predominantly isolated from the family Moraceae. Previously, studies demonstrated the health benefits of morin using human and animal models. Despite its importance as a bioactive compound, the genetic architecture of the morin biosynthetic pathway is still unclear. OBJECTIVE: To understand the genetic architecture of the morin biosynthetic pathway, the following components were analyzed: (1) cis-responsive element (CRE)-mediated regulation, (2) microRNAs (miRNA)-mediated post-transcriptional silencing, and (3) tissue-specific in silico gene expression. METHODS: To understand the genetic architecture of morin biosynthetic pathway, in silico survey was carried out using different web servers (MorusDB, MEME suite, NCBI database, PlantCARE, and psRNATarget) and collected mRNA, protein sequences, and expressed microarray data. TBtools was employed for depicting protein and promoter motifs and the heatmap preparation of tissue-specific expression of genes involved in the morin biosynthesis. RESULTS: The current data mining study highlighted the morin biosynthetic pathway associated genes, namely, phenylalanine ammonia-lyase (MnPAL), chalcone synthase A (MnCSA), chalcone-flavonone isomerase (MnCFI), and flavonoid 3′,5′-hydroxylase (MnFH) are transcriptionally regulated by different growth, development, and stress-responsive CREs. Differential expression profiles how MnPAL (L484_024373) and MnCFI (L484_011241) genes were upregulated across selected tissues. Moreover, miRNA-mediated post-transcriptional silencing was identified. CONCLUSIONS: This study will improve our understanding of morin biosynthesis, thus helping improve production via metabolic engineering.
{"title":"Genetic architecture of morin (pentahydroxyflavone) biosynthetic pathway in mulberry (Morus notabilis): an in silico approach","authors":"Raju Mondal, Sreya Antony, M. C. Thriveni, G. Thanavendan, G. Ravikumar, B. Sreenivasa","doi":"10.3233/jbr-220032","DOIUrl":"https://doi.org/10.3233/jbr-220032","url":null,"abstract":"BACKGROUND: Morin, (3,5,7,2′,4′-pentahydroxyflavone), is a polyphenolic compound belonging to bio-flavonoids and is predominantly isolated from the family Moraceae. Previously, studies demonstrated the health benefits of morin using human and animal models. Despite its importance as a bioactive compound, the genetic architecture of the morin biosynthetic pathway is still unclear. OBJECTIVE: To understand the genetic architecture of the morin biosynthetic pathway, the following components were analyzed: (1) cis-responsive element (CRE)-mediated regulation, (2) microRNAs (miRNA)-mediated post-transcriptional silencing, and (3) tissue-specific in silico gene expression. METHODS: To understand the genetic architecture of morin biosynthetic pathway, in silico survey was carried out using different web servers (MorusDB, MEME suite, NCBI database, PlantCARE, and psRNATarget) and collected mRNA, protein sequences, and expressed microarray data. TBtools was employed for depicting protein and promoter motifs and the heatmap preparation of tissue-specific expression of genes involved in the morin biosynthesis. RESULTS: The current data mining study highlighted the morin biosynthetic pathway associated genes, namely, phenylalanine ammonia-lyase (MnPAL), chalcone synthase A (MnCSA), chalcone-flavonone isomerase (MnCFI), and flavonoid 3′,5′-hydroxylase (MnFH) are transcriptionally regulated by different growth, development, and stress-responsive CREs. Differential expression profiles how MnPAL (L484_024373) and MnCFI (L484_011241) genes were upregulated across selected tissues. Moreover, miRNA-mediated post-transcriptional silencing was identified. CONCLUSIONS: This study will improve our understanding of morin biosynthesis, thus helping improve production via metabolic engineering.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49331635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hannah Arnold, G. Dobson, Alexandre Foito, C. Austin, J. Sungurtas, J. W. Allwood, D. Stewart, G. McDougall
BACKGROUND: Berry pomaces obtained after commercial juice production contain phytochemicals which may find use as antioxidants, food additives and biomedical products. Oil extraction from seeds provides additional value but the availability of phytochemicals before and after oil extraction is not well established. OBJECTIVE: This pilot study defines the content and composition of phytochemicals from raspberry and black currant pomaces after extraction with water-ethanol mixes, but also before and after milling/ oil extraction. METHODS: The total phenol (TPC), total anthocyanin (TAC) and antioxidant content of extracts was assessed. Their phytochemical composition was studied using liquid chromatography-mass spectrometry (LC-MSn). RESULTS: TPC and TAC increased with increasing % ethanol. Anthocyanins were major components in blackcurrant pomace and were more readily extracted than total phenols. Total oil content and composition was not influenced by solvent pre-extraction. Milling/ oil extraction markedly increased TPC from raspberry but not from blackcurrant pomace. LC-MSn confirmed characteristic phytochemical compositions and that increasing % ethanol increased yield of certain components. Milling increased specific ellagitannins, proanthocyanins and triterpenoids from raspberry. CONCLUSIONS: Milling/ oil extraction increased the yield and phytochemical diversity of extracts from raspberry but not from blackcurrant pomace which suggests that the phytochemicals from blackcurrant pomace are largely available on the pomace surfaces.
{"title":"Assessing available phytochemicals from commercial blackcurrant and raspberry pomaces","authors":"Hannah Arnold, G. Dobson, Alexandre Foito, C. Austin, J. Sungurtas, J. W. Allwood, D. Stewart, G. McDougall","doi":"10.3233/jbr-220017","DOIUrl":"https://doi.org/10.3233/jbr-220017","url":null,"abstract":"BACKGROUND: Berry pomaces obtained after commercial juice production contain phytochemicals which may find use as antioxidants, food additives and biomedical products. Oil extraction from seeds provides additional value but the availability of phytochemicals before and after oil extraction is not well established. OBJECTIVE: This pilot study defines the content and composition of phytochemicals from raspberry and black currant pomaces after extraction with water-ethanol mixes, but also before and after milling/ oil extraction. METHODS: The total phenol (TPC), total anthocyanin (TAC) and antioxidant content of extracts was assessed. Their phytochemical composition was studied using liquid chromatography-mass spectrometry (LC-MSn). RESULTS: TPC and TAC increased with increasing % ethanol. Anthocyanins were major components in blackcurrant pomace and were more readily extracted than total phenols. Total oil content and composition was not influenced by solvent pre-extraction. Milling/ oil extraction markedly increased TPC from raspberry but not from blackcurrant pomace. LC-MSn confirmed characteristic phytochemical compositions and that increasing % ethanol increased yield of certain components. Milling increased specific ellagitannins, proanthocyanins and triterpenoids from raspberry. CONCLUSIONS: Milling/ oil extraction increased the yield and phytochemical diversity of extracts from raspberry but not from blackcurrant pomace which suggests that the phytochemicals from blackcurrant pomace are largely available on the pomace surfaces.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46842805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Antonio Gurrola-Rios, E. Cuevas-Rodríguez, E. Reynoso-Soto, J. Milán-Carrillo, G. López-Angulo, M. Leyva-Acuña, J. Montes-Ávila
BACKGROUND: The green synthesis of nanoparticles based on noble metals has been researched in the last decades. The physical and optical properties of gold, along with the metabolites found in plant extracts that work as capping agents, allow the development of materials that can be used in alternative therapies in human health. OBJECTIVE: This study reports the obtention of gold nanoparticles (AuNPs) through a green synthesis process, using purified anthocyanins (ACNs) from Rubus palmeri berries as reducing and stabilizing agents. RESULTS: The colloidal solution obtained with a ratio of 1.6 mg: 0.6 mM (ACNs:HAuCl4) was monitored through UV-Vis spectroscopy over 10 h at 30°C, producing a surface plasmon resonance (SPR) signal at 540 nm. The interaction between the ACNs and the AuNPs was analyzed through Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) determined an average size of 13 nm with a spherical morphology predominance at 30°C, while at 20°C triangular morphologies appeared. Energy-dispersive X-ray spectroscopy (EDX) and elemental mapping showed predominance of the gold element. The antioxidant activity was analyzed by ORAC and ABTS assays, showing a variation of up to four times the EC50 between the AuNPs and the ACNs. CONCLUSION: These results determined the role of ACNs in the green synthesis of AuNPs and their capability to induce various morphologies at different temperatures.
背景:近几十年来，以贵金属为基材的纳米颗粒绿色合成技术得到了广泛的研究。黄金的物理和光学特性，以及在植物提取物中发现的代谢物作为盖层剂，使人们能够开发出可用于人类健康替代疗法的材料。目的:研究以棕榈莓中纯化的花青素(ACNs)为还原剂和稳定剂，通过绿色合成金纳米颗粒(AuNPs)。结果:以1.6 mg: 0.6 mM (ACNs:HAuCl4)的比例获得的胶体溶液在30°C下，通过紫外可见光谱监测10 h，在540 nm处产生表面等离子体共振(SPR)信号。利用傅里叶变换红外光谱(FTIR)分析了acn与aunp之间的相互作用。扫描电子显微镜(SEM)和透射电子显微镜(TEM)发现，在30°C时平均尺寸为13 nm，以球形形貌为主，而在20°C时则出现三角形形貌。能量色散x射线能谱(EDX)和元素图显示金元素占主导地位。通过ORAC和ABTS分析抗氧化活性，发现aunp和acn之间的EC50差异高达4倍。结论:这些结果确定了acn在绿色合成AuNPs中的作用及其在不同温度下诱导各种形态的能力。
{"title":"Green synthesis and characterization of gold nanoparticles using anthocyanins from Rubus palmeri","authors":"José Antonio Gurrola-Rios, E. Cuevas-Rodríguez, E. Reynoso-Soto, J. Milán-Carrillo, G. López-Angulo, M. Leyva-Acuña, J. Montes-Ávila","doi":"10.3233/jbr-220021","DOIUrl":"https://doi.org/10.3233/jbr-220021","url":null,"abstract":"BACKGROUND: The green synthesis of nanoparticles based on noble metals has been researched in the last decades. The physical and optical properties of gold, along with the metabolites found in plant extracts that work as capping agents, allow the development of materials that can be used in alternative therapies in human health. OBJECTIVE: This study reports the obtention of gold nanoparticles (AuNPs) through a green synthesis process, using purified anthocyanins (ACNs) from Rubus palmeri berries as reducing and stabilizing agents. RESULTS: The colloidal solution obtained with a ratio of 1.6 mg: 0.6 mM (ACNs:HAuCl4) was monitored through UV-Vis spectroscopy over 10 h at 30°C, producing a surface plasmon resonance (SPR) signal at 540 nm. The interaction between the ACNs and the AuNPs was analyzed through Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) determined an average size of 13 nm with a spherical morphology predominance at 30°C, while at 20°C triangular morphologies appeared. Energy-dispersive X-ray spectroscopy (EDX) and elemental mapping showed predominance of the gold element. The antioxidant activity was analyzed by ORAC and ABTS assays, showing a variation of up to four times the EC50 between the AuNPs and the ACNs. CONCLUSION: These results determined the role of ACNs in the green synthesis of AuNPs and their capability to induce various morphologies at different temperatures.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42163025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:The sweetness of highbush blueberries (Vaccinium corymbosum L.) is related to changes in sucrose and other primary sugar metabolites. OBJECTIVE:This study compared the fruit quality and sugar metabolism in the blueberry cultivars Brigitta and Elizabeth at three ripening stages and with apostharvest chitosan treatment to determine which factors are important in sugar metabolism in the two cultivars. METHODS:Blueberries were harvested at three ripening stages. Fully ripe fruit was treated with chitosan and kept at room temperature for 5 and 10 days. GC-MS was used to measure organic acids, individual sugars, and sugar derivatives. Gene expression was analysed using qPCR. RESULTS:Elizabeth fruit was less firm than Brigitta fruit, but contained more soluble solids. The sweeter taste of Elizabeth versus Brigitta was due to down-regulated VcINV, VcSPS, and VcADR transcription and increased sucrose concentration. Chitosan inhibited VcADR and VcINV transcription in Elizabeth, but activated these genes in Brigitta after 5 days of storage. CONCLUSIONS:Aldose reductase might be an essential enzyme in blueberry sugar metabolism. These findings support research on the mechanism of sugar accumulation in fruit during maturation and after postharvest treatments in blueberries.
{"title":"Changes in sucrose and the VcADR gene induced sugar accumulation in blueberries during ripening and after chitosan treatment","authors":"Chau Thi Thu Nguyen, Seung-Ok Yang, Won-Sil Choi","doi":"10.3233/jbr-220016","DOIUrl":"https://doi.org/10.3233/jbr-220016","url":null,"abstract":"BACKGROUND:The sweetness of highbush blueberries (Vaccinium corymbosum L.) is related to changes in sucrose and other primary sugar metabolites. OBJECTIVE:This study compared the fruit quality and sugar metabolism in the blueberry cultivars Brigitta and Elizabeth at three ripening stages and with apostharvest chitosan treatment to determine which factors are important in sugar metabolism in the two cultivars. METHODS:Blueberries were harvested at three ripening stages. Fully ripe fruit was treated with chitosan and kept at room temperature for 5 and 10 days. GC-MS was used to measure organic acids, individual sugars, and sugar derivatives. Gene expression was analysed using qPCR. RESULTS:Elizabeth fruit was less firm than Brigitta fruit, but contained more soluble solids. The sweeter taste of Elizabeth versus Brigitta was due to down-regulated VcINV, VcSPS, and VcADR transcription and increased sucrose concentration. Chitosan inhibited VcADR and VcINV transcription in Elizabeth, but activated these genes in Brigitta after 5 days of storage. CONCLUSIONS:Aldose reductase might be an essential enzyme in blueberry sugar metabolism. These findings support research on the mechanism of sugar accumulation in fruit during maturation and after postharvest treatments in blueberries.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"26 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Jesús Tejada-Alvarado, Jegnes Benjamín Meléndez-Mori, N. C. Vilca-Valqui, Eyner Huaman-Huaman, Yoiner K. Lapiz-Culqui, Juan C. Neri, Maria Loreto Prat, M. Oliva
BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior. CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.
{"title":"Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization","authors":"José Jesús Tejada-Alvarado, Jegnes Benjamín Meléndez-Mori, N. C. Vilca-Valqui, Eyner Huaman-Huaman, Yoiner K. Lapiz-Culqui, Juan C. Neri, Maria Loreto Prat, M. Oliva","doi":"10.3233/jbr-211565","DOIUrl":"https://doi.org/10.3233/jbr-211565","url":null,"abstract":"BACKGROUND: Blueberry production has generated great commercial expectations, therefore for its agricultural expansion it is necessary to overcome the challenges at the time of mass propagation. OBJECTIVE: Evaluate the effect of a set of factors influencing micropropagation, as well as the influence of substrates on the ex vitro morpho-physiological performance of blueberry seedlings. METHODS: A set of protocols were developed to optimize all stages of micropropagation (aseptic establishment, multiplication, rooting, and acclimatization) of blueberries. RESULTS: Explants immersed in 1.5% NaClO for 8 min and then in 0.1% HgCl2 for 2 min achieved 100% sterility and a viability rate of 86.67% for ‘Biloxi’ and 93.33% for ‘Bluecrop’. At the multiplication stage, the maximum number of shoots of ‘Biloxi’ (3.53) and ‘Bluecrop’ (2.27) were obtained on the medium supplemented with 0.2 and 10 mg L–1 silver nanoparticles (AgNPs), respectively. The percentage of in vitro rooting was significantly improved on media containing activated charcoal, with levels between 80% and 100% . In the acclimatization phase, plants grown in a substrate composed of peat and cocomix® (2:1 ratio) showed greater uniformity and better morpho-physiological behavior. CONCLUSIONS: The present results could be successfully used for large-scale commercial production of blueberries of the varieties ‘Biloxi’ and ‘Bluecrop’.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47371772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND: As the interest in foods with positive effects on human health has increased in recent decades, the importance of the mineral contents and oil compositions of grape seeds has been better understood. However, research on grape seeds to date has largely focused on effects on health and the usability of grape seeds in the food industry and animal feeds. In agricultural research, grape seeds have generally been evaluated as a source of genes in breeding new varieties and as propagation material. OBJECTIVE: Unlike previous studies, this study not only investigates the effect of variety on the changes of pomological properties, fatty acid compositions, and oil, protein, and mineral contents of grape seeds; it also aims to determine the effects of grape rootstocks and growing seasons on those changes. METHODS: The seeds of five grape varieties (Red Globe, Trakya Ilkeren, Ata Sarisi, Hatun Parmagi, and Horoz Karasi) grafted onto drought-tolerant grape rootstocks (1103P and 110R) were analyzed during the growing seasons of 2017 and 2018. RESULTS: Seed size, moisture and oil contents, major fatty acids (palmitic, oleic, and linoleic), and levels of saturated fatty acids, monounsaturated fatty acids, unsaturated fatty acids, and boron varied by variety, rootstock, and season. However, the effects of growing seasons and varieties on the seeds were not limited to these findings; effects were also seen for seed vitality, protein content, and N, K, Ca, and Fe levels of the seeds, among others. CONCLUSIONS: Genotype was found to be the main factor causing changes in the fatty acid compositions and mineral contents of grape seeds. When the factors that we investigated were ordered based on the changes they caused for seeds in general, the sequence that appeared was genotype > growing season > grape rootstock.
{"title":"Effects of drought-tolerant grapevine rootstocks on the mineral contents and fatty acid compositions of grape seeds","authors":"M. I. Odabasioglu, S. Gürsöz","doi":"10.3233/jbr-220007","DOIUrl":"https://doi.org/10.3233/jbr-220007","url":null,"abstract":"BACKGROUND: As the interest in foods with positive effects on human health has increased in recent decades, the importance of the mineral contents and oil compositions of grape seeds has been better understood. However, research on grape seeds to date has largely focused on effects on health and the usability of grape seeds in the food industry and animal feeds. In agricultural research, grape seeds have generally been evaluated as a source of genes in breeding new varieties and as propagation material. OBJECTIVE: Unlike previous studies, this study not only investigates the effect of variety on the changes of pomological properties, fatty acid compositions, and oil, protein, and mineral contents of grape seeds; it also aims to determine the effects of grape rootstocks and growing seasons on those changes. METHODS: The seeds of five grape varieties (Red Globe, Trakya Ilkeren, Ata Sarisi, Hatun Parmagi, and Horoz Karasi) grafted onto drought-tolerant grape rootstocks (1103P and 110R) were analyzed during the growing seasons of 2017 and 2018. RESULTS: Seed size, moisture and oil contents, major fatty acids (palmitic, oleic, and linoleic), and levels of saturated fatty acids, monounsaturated fatty acids, unsaturated fatty acids, and boron varied by variety, rootstock, and season. However, the effects of growing seasons and varieties on the seeds were not limited to these findings; effects were also seen for seed vitality, protein content, and N, K, Ca, and Fe levels of the seeds, among others. CONCLUSIONS: Genotype was found to be the main factor causing changes in the fatty acid compositions and mineral contents of grape seeds. When the factors that we investigated were ordered based on the changes they caused for seeds in general, the sequence that appeared was genotype > growing season > grape rootstock.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41347899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUND:Abundant germplasm resources of Vaccinium uliginosum are present in Changbai Mountain in China, which have considerable research and development value. They include the naturally distributed alpine V. uliginosum, the white-fruited V. uliginosum variety, and various fruit shapes dominatedby ellipses. However, the relevant research is limited, and the taxonomic status of various specific germplasm resources has not been addressed. OBJECTIVE:The genetic relationship and taxonomic status of four species of V. uliginosum germplasm resources in Changbai Mountain were investigated. METHODS:The chloroplast matK gene and SRAP molecular markers of 94 V. uliginosum samples were analyzed. RESULTS:According to the analysis of chloroplast matK gene, all four types were divided into three branches. The results showed low variation among natural samples of V. uliginosum in China and a relationship between haplotype distribution and geographical distribution. The results of the SRAP molecular marker analysis divided the V. uliginosum population into five branches. The multiple individuals of the same trait were clustered together, and different partial samples were clustered together. CONCLUSIONS:Diploid alpine V. uliginosum and tetraploid V. uliginosum were identified as two subspecies of V. uliginosum. The elliptical fruit shapewas the variant of tetraploid round blue fruit V. uliginosum, and the white-fruited V. uliginosum variety is a variant.
{"title":"Taxonomic status of intraspecific germplasm resources of Vaccinium uliginosum based on chloroplast matK gene and SRAP molecular markers","authors":"Jia-Zhuo Chen, Tong Li, Xin Tian, Yu-Hang Zheng, Zhen-Ling Jin, Hou-Nan Cao, Cheng-Wen Zong","doi":"10.3233/jbr-211559","DOIUrl":"https://doi.org/10.3233/jbr-211559","url":null,"abstract":"BACKGROUND:Abundant germplasm resources of Vaccinium uliginosum are present in Changbai Mountain in China, which have considerable research and development value. They include the naturally distributed alpine V. uliginosum, the white-fruited V. uliginosum variety, and various fruit shapes dominatedby ellipses. However, the relevant research is limited, and the taxonomic status of various specific germplasm resources has not been addressed. OBJECTIVE:The genetic relationship and taxonomic status of four species of V. uliginosum germplasm resources in Changbai Mountain were investigated. METHODS:The chloroplast matK gene and SRAP molecular markers of 94 V. uliginosum samples were analyzed. RESULTS:According to the analysis of chloroplast matK gene, all four types were divided into three branches. The results showed low variation among natural samples of V. uliginosum in China and a relationship between haplotype distribution and geographical distribution. The results of the SRAP molecular marker analysis divided the V. uliginosum population into five branches. The multiple individuals of the same trait were clustered together, and different partial samples were clustered together. CONCLUSIONS:Diploid alpine V. uliginosum and tetraploid V. uliginosum were identified as two subspecies of V. uliginosum. The elliptical fruit shapewas the variant of tetraploid round blue fruit V. uliginosum, and the white-fruited V. uliginosum variety is a variant.","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":"12 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138539069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ping Wang, Jing Yang, Zi-Yi Li, Jia Zhu, Q. Gao, D. Ni, K. Duan
{"title":"Erratum to: Genome-wide identification and expression analysis revealed cinnamyl alcohol dehydrogenase genes correlated with fruit-firmness in strawberry","authors":"Ping Wang, Jing Yang, Zi-Yi Li, Jia Zhu, Q. Gao, D. Ni, K. Duan","doi":"10.3233/jbr-220950","DOIUrl":"https://doi.org/10.3233/jbr-220950","url":null,"abstract":"","PeriodicalId":15194,"journal":{"name":"Journal of Berry Research","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2022-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48832689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：