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Effects of Probiotic Bacillus on Growth Performance, Immune Response and Disease Resistance in Aquaculture 益生菌对水产养殖生长性能、免疫反应和抗病性的影响
Pub Date : 2021-03-02 DOI: 10.20944/PREPRINTS202103.0075.V1
Ashikur Rahman, S. H. T. Shefat, M. A. Chowdhury
Aquaculture is one of the fastest-growing animal food-producing agricultural industries in the world and proper performance of fish in morphological, physiological and immunological aspects is important for fish production and sustainable expansion of aquaculture. But several inhibitors like disease, pathogen, and adverse environment can overpower these performances. At present, antibiotics in preventing these inhibitors have been seen as becoming favorable to those inhibitors. So, Bacillus, an important group of probiotic bacteria can be an alternative to these antibiotics in aquaculture. Bacillus has been seen used in different experiments, mainly as a supplement in feed at various concentrations. Bacillus showed effective results like improved growth with minimum cost, improvement in reproduction, hematology, improved immune response and disease, and stress resistance as well as better proximate composition in different fish species. Application of Bacillus strains has proven efficient in improving water quality by reducing ammonia and nitrite toxicity, harmful algal blooms and utilization of H+ ion. Larger application of probiotic Bacillus instead of the hazardous synthetic chemicals would promote eco-friendly low-input sustainable aquaculture for food and nutritional security of the increasing world population. So many more experiments should be conducted in commercially important fishes for better growth and health of fishes which will certainly increase fish
水产养殖是世界上发展最快的动物食品生产农业产业之一,鱼类在形态、生理和免疫方面的良好性能对鱼类生产和水产养殖的可持续发展至关重要。但一些抑制因素,如疾病、病原体和不利的环境,可以压制这些表现。目前,抗生素在预防这些抑制剂方面已被视为对这些抑制剂有利。因此,芽孢杆菌是一种重要的益生菌群,可以在水产养殖中替代这些抗生素。芽孢杆菌已在不同的实验中使用,主要作为不同浓度的饲料补充物。芽孢杆菌在不同鱼类中表现出以最小成本促进生长、改善繁殖、改善血液学、改善免疫反应和疾病、改善抗逆性以及改善近似组成等有效效果。应用芽孢杆菌菌株通过降低氨和亚硝酸盐毒性,减少有害藻华和利用H+离子来改善水质。大量应用益生菌芽孢杆菌代替有害的合成化学品将促进生态友好、低投入的可持续水产养殖,为日益增长的世界人口提供粮食和营养安全。因此,应该在商业上重要的鱼类身上进行更多的实验,以促进鱼类的生长和健康,这肯定会增加鱼类的数量
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引用次数: 7
Effect of Growth Temperature on Muscle Lipid Class and Fatty Acid Composition in Adult Steelhead Trout (Oncorhynchus mykiss) Fed Commercial Diets with Different andomega;6 to andomega;3 Fatty Acid Ratios 生长温度对不同- 6与- 3脂肪酸比例商业饲料对成年虹鳟肌脂类和脂肪酸组成的影响
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.643
M. Wijekoon, Parrish Cc, A. Mansour
The effect of fluctuating temperature on muscle lipid composition was examined in 1.8 Kg steelhead trout fed 3 commercial diets with varying proportions (16.5 - 31% total) of Σω3 fatty acids and large differences (5×) in ω6:ω3 fatty acid ratios. Temperature was increased from 13.5°C to 18.0°C and then dropped back down to 13.5°C over a period of 12 weeks. Diet had no significant effect on growth or on total polyunsaturated fatty acid (PUFA) proportions in muscle tissue, but significant changes were observed in individual fatty acids. There was a greater response to changes in diet in the fatty acids than there was to changes in temperature. Muscle C20–C22 ω3PUFA and C18 unsaturated fatty acids reflected dietary inputs leading to very different (up to 40%) ω6:ω3 fatty acid ratios. Independent of diet, increasing temperature significantly increased total lipids and saturated fatty acids, especially 16:0, and decreased monounsaturated ones, especially 18:1ω9, as well as the PUFA 18:2ω6. The ω6:ω3 fatty acid ratio was also lowest at 18.0°C. Increasing total lipids, sterols and saturated fatty acids with increasing temperature, has important implications for both cultured and wild fish in terms of their nutrition, food quality for humans, and resource availability for aquafeeds.
研究了温度波动对1.8 Kg钢头鳟鱼肌肉脂质组成的影响。该鱼饲喂了3种不同比例(16.5 ~ 31%)Σω3脂肪酸和ω6:ω3脂肪酸比例差异较大(5倍)的商业饲料。温度从13.5°C升高到18.0°C,然后在12周的时间内降回13.5°C。饲料对生长和肌肉组织中总多不饱和脂肪酸(PUFA)比例无显著影响,但对个别脂肪酸有显著影响。饮食变化对脂肪酸的影响比对温度变化的影响更大。肌肉中C20-C22 ω3PUFA和C18不饱和脂肪酸反映了膳食输入导致ω6:ω3脂肪酸比例差异很大(高达40%)。与饮食无关,温度升高显著提高了总脂质和饱和脂肪酸含量,尤其是16:0,显著降低了单不饱和脂肪酸含量,尤其是18:1ω9,显著降低了PUFA含量18:2ω6。ω6:ω3脂肪酸比值在18.0℃时也最低。随着温度的升高,总脂质、甾醇和饱和脂肪酸的增加对养殖鱼类和野生鱼类的营养、人类食品质量和水产饲料资源的可用性都具有重要意义。
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引用次数: 0
Growth Performance and Survival Rates of Milkfish (Chanos chanos) Fed Diets Supplemented with Pineapple Extract. 添加菠萝提取物对遮目鱼生长性能和成活率的影响。
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.637
Kiki Wahyu Nilamsari, J. Hutabarat, D. Rachmawati, Bayu Prakoso Adi
Milkfish (Chanos chanos) is one of commodity which is strategic to fulfill necessity of protein that cheap enough and delighted by customer in Indonesian. The low feed efficiency is one of the obstacles in milkfish intensive farming. This can be overcome by the addition of the exogenous enzyme that an important role to digestion of feed, among exogenous enzyme is bromelain enzyme contained in pineapple extract. Enzyme bromelain is one of enzyme protease, which has the function to dissolve the protein with hydrolyzing of peptide in acid amino. This experiment has purpose to know the influence giving pineapple extract on artificial feed to feed utilization efficiency, growth, and survival rate of milkfish (C. chanos). Fish samples used 42-day old C. chanos with an average weight of 3.54 ± 0.03 g. This research used completely randomized experimental and design methods (RAL) with 4 treatments and 3 replications, respectively. The used treatments were treatment A (0 ml), B (2.5 ml), C (5 ml), and D (7.5 ml). The results showed that the proportion of pineapple extract was very significant influence (P 0.01) to Total Feed Consumed(TKP) and Survival Rate(SR). The optimum dose of pineapple extract is 4.50 ml can be Produce EPP maximum about 52.73%. The dose optimum pineapple extract about 4.26 ml can be produce per in maximum 1.55% and the optimum dose of pineapple extract 4.39 ml can produce RGR about 3.22%.
遮目鱼(Chanos Chanos)是满足印尼消费者对蛋白质需求的战略性商品之一,价格低廉,深受消费者喜爱。饲料效率低是遮目鱼集约化养殖的障碍之一。这可以通过添加外源酶来克服,外源酶对饲料的消化起重要作用,其中菠萝提取物中含有菠萝蛋白酶酶。菠萝蛋白酶是蛋白酶的一种,具有水解氨基酸多肽溶解蛋白质的功能。本试验旨在了解添加菠萝提取物对遮目鱼饲料利用效率、生长和存活率的影响。鱼样选用42日龄查诺鱼,平均体重为3.54±0.03 g。本研究采用完全随机试验和设计方法(RAL),分别设4个处理,3个重复。采用处理A (0 ml)、处理B (2.5 ml)、处理C (5 ml)、处理D (7.5 ml)。结果表明:菠萝提取物的添加比例对饲料总耗料(TKP)和成活率(SR)有极显著影响(p0.01)。菠萝提取物的最佳剂量为4.50 ml,可产生EPP,最高可达52.73%。以4.26 ml菠萝提取物为最佳剂量时,RGR最高可达1.55%;以4.39 ml为最佳剂量时,RGR最高可达3.22%。
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引用次数: 0
Combination of Aquaculture and Hydroponics 水产养殖与水培相结合
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.655
Rakshitha Kotha
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引用次数: 0
Nutrient Supplementation to Aquatic Species and their Metabolic Growth 水生物种的营养补充及其代谢生长
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.657
Rakshitha Kotha
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引用次数: 0
Commercial Phase of Hatchery System 孵化系统的商业阶段
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.656
Rakshitha Kotha
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引用次数: 0
Formulation of Diet Using Conventional and Non-Conventional Protein Sources 使用常规和非常规蛋白质来源的日粮配方
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.648
Maria Lizanne Ac, Jeana Ida Rodrigues, Miriam Triny Fern, Es
A 60-day feeding experiment was conducted on Swordtail, Xiphophorus helleri to correlate the growth and the crude protein percentage of the feed. Nine experimental diets (Treatments) were formulated using different locally and cheaply available conventional and nonconventional protein sources keeping the basal ingredients same. These diets were tested on three replicate groups of 10 fishes (initial body weight: 0.7 ± 0.5 g) bred in circular fiber reinforced plastic tanks of 120 liter capacity with 100 liter of seasoned de-chlorinated tap water. Fishes were fed 3% of their body weight. The growth performance of Swordtail was studied in terms of Weight Gain, Feed Conversion Ratio (FCR), Specific Growth Rate (SGR%/day) and Protein Efficiency Ratio (PER). The results indicated that Weight Gain was more in the Treatment that contained Chicken Waste and least in the Treatment that contained Marine Fish Waste. Specific growth rate%/per day and Protein efficiency ratio showed similar results. The Feed conversion ratio was greater in Treatment that contained Marine Fish Waste and least in Treatment that contained Chicken Waste. The study suggests that Chicken Waste as a major source of Protein can be effectively considered in the formulation of practical diet for better growth of Swordtail, Xiphophorus helleri.
本试验对剑尾、剑尾鱼进行了为期60 d的饲养试验,研究了剑尾鱼生长与饲料粗蛋白质含量的关系。9种试验饲粮(处理)采用不同的当地廉价常规和非常规蛋白质来源配制,基本成分相同。试验采用3个重复组,每组10尾鱼(初始体重:0.7±0.5 g),在容量为120升的圆形纤维增强塑料水箱中饲养,并加入100升调味过的去氯自来水。鱼被喂食体重的3%。以增重、饲料系数(FCR)、特定生长率(SGR%/d)和蛋白质效率(PER)为指标研究剑尾鱼的生长性能。结果表明,鸡粪处理的增重较大,海鱼粪处理的增重较小。特定生长率%/ d和蛋白质效率也有相似的结果。含海鱼废物处理的饲料转化率较高,含鸡废物处理的饲料转化率最低。本研究提示,为促进剑尾鱼的生长,可在实际饲料配方中有效考虑鸡粪作为蛋白质的主要来源。
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引用次数: 0
Morphological and Pathogenic Variability in Different Isolates of Fusarium oxysporum f. sp. cumini 黄瓜尖孢镰刀菌不同分离株的形态及致病变异
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.646
S. Choudhary, S. Godika
Wilt of cumin incited by Fusarium oxysporum f. sp. cumini is one of the important disease and a big constraint in successful cultivation.  Therefore, in the present study, 7 isolates of Fusarium oxysporum f. sp. cumini collected from different cumin growing areas of Rajasthan were assessed for morphological and pathogenic variations. The different isolates produced sickle shaped macro conidia and spores of varying width, length having variable number of septa. The isolate I2 produced highest length and number of septa in macro conidia. The length of macro conidia varied from 22.77 µm in isolate I3 to 29.65 µm in isolate I2 whereas, micro conidia length varied from 7.54 µm in I4 to 11.53 µm in I6. In study of pathogenic variability, among different isolate, the isolate I1 proved most virulent as it showed disease symptoms earliest whereas, isolate I5 proved least virulent.
小茴香枯萎病(Fusarium oxysporum f. sp. cumini)是小茴香的重要病害之一,也是成功栽培的一大制约因素。因此,本研究对从拉贾斯坦邦不同孜然种植区采集的7株枯镰刀菌进行了形态和致病变异分析。不同的分离株产生镰刀状的大分生孢子和孢子,孢子的宽度、长度和间隔数不等。分离物I2在大分生孢子中产生的间隔长度和数量最多。分离物I3的大分生孢子长度为22.77µm ~ 29.65µm,分离物I2的小分生孢子长度为7.54µm ~ 11.53µm。在致病变异性的研究中,不同的分离物中,分离物I1表现出最早的发病症状,毒力最强,而分离物I5表现出最低的毒力。
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引用次数: 0
Growth and Yield Response of Food Barley (Hordeum vulgare L.) Varieties to Nitrogen Fertilizer Rates Inkofele District, Southeastern Oromia 食用大麦的生长与产量响应奥罗米亚东南部Inkofele地区氮肥品种比率
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.645
KedirJemal, Mohammed Aliyi
Despite the importance of barely, there are several factors affecting its production. Among these inadequate uses of N fertilizer and lack of using improved food barley variety are the most important ones. A field experiment was conducted under rainy season conditions to investigate the effects of food barley varieties and rates of nitrogen fertilizer application in the combinations of three food barley varieties, namely Adoshe, Robera and Abdene and four levels of N (23 Kg N/ha , 46 Kg N/ha , 69 Kg N/ha  and 92 Kg N/ha) arranged in randomized complete block design in factorial arrangement with three replications on vegetative growth, yield and yield components of barley ( Hordeum vulgare L.) at Kofele, Oromia Regional State of Ethiopia. Results indicated that different nitrogen fertilizer rate had a very highly significant (p 0.05). Moreover, barley phonological, growth, yield and yield components were highly significantly (p 0.05). the maximum and minimum mean values of grain yield production of barley were observed from Adoshe barley variety treated with 92 Kg ha-1 nitrogen fertilizer rate (6.01 tone /ha) and Robera barley variety treated with 23 Kg ha-1 nitrogen fertilizer rate (3.29 tone/ha), respectively. The partial budget analysis revealed that, maximum net benefit of Birr 99350.00 ha-1 with an acceptable marginal rate of returns (MRR) of 3348.23% with the treatment Adoshe variety with combination of 92 Kg N ha-1. However, the lowest net benefit of (Birr 55225.00 ha-1) was recorded from Robera barley variety treated with 23 Kg ha-1 nitrogen fertilizer rate. Therefore, Production of Adoshe variety with combination of 92 Kg N ha-1 was economical, and uncertainly recommended for production of food barley in the study area and other areas with similar agro-ecological condition.
尽管勉强的重要性,有几个因素影响它的生产。其中氮肥施用不足和改良食用大麦品种使用不足是最主要的原因。本试验在雨季条件下,采用随机完全区组设计,3个重复,研究了阿多施、罗伯A和阿伯丁3个食用大麦品种和4个N水平(23 Kg N/ha、46 Kg N/ha、69 Kg N/ha和92 Kg N/ha)组合施氮量对小麦营养生长的影响。埃塞俄比亚奥罗米亚州科菲勒地区大麦(Hordeum vulgare L.)的产量和产量组成部分。结果表明,不同施氮量对青枯病的影响极显著(p < 0.05)。此外,大麦的音系、生长、产量和产量组成均极显著(p 0.05)。92 Kg hm -1施氮量(6.01吨/公顷)处理的阿多什和23 Kg hm -1施氮量(3.29吨/公顷)处理的大麦籽粒产量均值最高和最小。局部预算分析结果表明,与阿多什品种配合92 Kg N ha-1处理,Birr 99350.00 ha-1的最大净效益为3348.23%,可接受边际收益率为3348.23%。而施氮量为23 Kg hm -1的罗伯大麦品种(Birr 55225.00 hm -1)的净效益最低。因此,92 Kg N / hm -1组合生产阿多舍品种是经济的,不确定推荐用于研究区和其他类似农业生态条件的地区生产食用大麦。
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引用次数: 2
Nursery Rearing and Seed Stocking of Silver Pompano 银鲳鱼苗圃饲养与种子放养
Pub Date : 2021-01-01 DOI: 10.35248/2155-9546.21.12.653
Rakshitha Kotha
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引用次数: 0
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Journal of Aquaculture Research and Development
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