M. Fan, Fei Xie, Jianfeng Li, Junquan Li, Ting Liang
This study explores the protective mechanism of cardiomyocyte exosome nanovesicles on myocardial infarction. Enzyme activity was measured and apoptosis of rat cardiomyocytes was assessed by TdT-mediated dUTP Nick-End Labeling (TUNEL) along with analysis of myocardial function by color Doppler echocardiogram. In addition, triphenyl tetrazolium chloride (TTC) staining evaluated the myocardial ischemia. BMSCs were co-cultured with primary cardiomyocytes followed by measuring autophagy and exosome morphology of myocardial tissue by transmission electron microscope and protein expression by Western blot. Exosomes secreted by Bone marrow mesenchymal stem cells (BMSCs) were successfully isolated. LDH, CK, CK-MB activity, cardiomyocyte apoptosis rate, LVESD, LVEDD, LVEDP, myocardial infarction area, cell area occupied by autophagic vesicles and p53 expression in model group were higher than control group (P <0.05) and they were lower in exosome group than model group. In addition, LVEF, LVFS, and LVSP were lower in model group and higher in exosome group (P <0.05). Model group showed significantly promoted autophagy activation and autophagosome formation, which were inhibited by cardiomyocyte exosomes. Moreover, exosome-like vesicles were found with a diameter of 60–90 nm. Furthermore, PKH-26 staining showed that cardiomyocytes could uptake exosomes well. Myocardial injury significantly promotes autophagy activation. Moreover, the apoptosis rate of cardiomyocytes and the area of myocardial ischemia were reduced and the cardiac function of rats with myocardial infarction was improved.
{"title":"Cardiomyocyte Exosomal Nanovesicles Mediating p53 to Regulate Autophagy and Protect Myocardial Infarction","authors":"M. Fan, Fei Xie, Jianfeng Li, Junquan Li, Ting Liang","doi":"10.1166/jbn.2023.3646","DOIUrl":"https://doi.org/10.1166/jbn.2023.3646","url":null,"abstract":"This study explores the protective mechanism of cardiomyocyte exosome nanovesicles on myocardial infarction. Enzyme activity was measured and apoptosis of rat cardiomyocytes was assessed by TdT-mediated dUTP Nick-End Labeling (TUNEL) along with analysis of myocardial function by color\u0000 Doppler echocardiogram. In addition, triphenyl tetrazolium chloride (TTC) staining evaluated the myocardial ischemia. BMSCs were co-cultured with primary cardiomyocytes followed by measuring autophagy and exosome morphology of myocardial tissue by transmission electron microscope and protein\u0000 expression by Western blot. Exosomes secreted by Bone marrow mesenchymal stem cells (BMSCs) were successfully isolated. LDH, CK, CK-MB activity, cardiomyocyte apoptosis rate, LVESD, LVEDD, LVEDP, myocardial infarction area, cell area occupied by autophagic vesicles and p53 expression in model\u0000 group were higher than control group (P <0.05) and they were lower in exosome group than model group. In addition, LVEF, LVFS, and LVSP were lower in model group and higher in exosome group (P <0.05). Model group showed significantly promoted autophagy activation and autophagosome\u0000 formation, which were inhibited by cardiomyocyte exosomes. Moreover, exosome-like vesicles were found with a diameter of 60–90 nm. Furthermore, PKH-26 staining showed that cardiomyocytes could uptake exosomes well. Myocardial injury significantly promotes autophagy activation. Moreover,\u0000 the apoptosis rate of cardiomyocytes and the area of myocardial ischemia were reduced and the cardiac function of rats with myocardial infarction was improved.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45138822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to explore the effect of orthokeratology (Ortho-K) combined with visual motor training in juvenile myopia. We included 156 myopia patients, which were randomly divided into the combined (visual motor training+Ortho-K) and Ortho-K (only) groups. Corneal morphological changes (horizontal keratometry [HK], vertical keratometry [VK], central corneal thickness [CCT], and corneal endothelial cell density), regulatory function (positive and negative relative accommodation [PRA, NRA], regulatory amplitude, and regulatory sensitivity), ocular axis, visual acuity (naked visual acuity, Diopter), tear film stability (BUT test and SIT test), intraocular pressure, and safety before and after treatment were compared between groups. Moreover, we performed a Spearman correlation analysis on the effects of initial age and diopter on axial growth. After treatment, HK, VK of cornea, and CCT levels significantly decreased in both groups while no significant changes were found in endothelial cell density. In the combined group, there was a higher improvement in PRA, accommodation amplitude, and accommodation sensitivity, which could delay ocular axis growth and improve naked eye vision and diopters. Meanwhile, a significant negative correlation was found between patients’ initial age and axial growth. Moreover, BUT and SIT recovery in the combined group were relatively better after wearing Ortho-K. In conclusion, Ortho-K combined with visual motor training could better correct the vision of juvenile myopia patients and delay ocular axis growth. In addition, the earlier the intervention, the better the effect.
{"title":"Orthokeratology and Visual Motor Training for Juvenile Myopia","authors":"Yao Chen, Jingjing Zhang, Hui Huang, Bo Su","doi":"10.1166/jbn.2023.3651","DOIUrl":"https://doi.org/10.1166/jbn.2023.3651","url":null,"abstract":"This study aimed to explore the effect of orthokeratology (Ortho-K) combined with visual motor training in juvenile myopia. We included 156 myopia patients, which were randomly divided into the combined (visual motor training+Ortho-K) and Ortho-K (only) groups. Corneal morphological\u0000 changes (horizontal keratometry [HK], vertical keratometry [VK], central corneal thickness [CCT], and corneal endothelial cell density), regulatory function (positive and negative relative accommodation [PRA, NRA], regulatory amplitude, and regulatory sensitivity), ocular axis, visual acuity\u0000 (naked visual acuity, Diopter), tear film stability (BUT test and SIT test), intraocular pressure, and safety before and after treatment were compared between groups. Moreover, we performed a Spearman correlation analysis on the effects of initial age and diopter on axial growth. After treatment,\u0000 HK, VK of cornea, and CCT levels significantly decreased in both groups while no significant changes were found in endothelial cell density. In the combined group, there was a higher improvement in PRA, accommodation amplitude, and accommodation sensitivity, which could delay ocular axis growth\u0000 and improve naked eye vision and diopters. Meanwhile, a significant negative correlation was found between patients’ initial age and axial growth. Moreover, BUT and SIT recovery in the combined group were relatively better after wearing Ortho-K. In conclusion, Ortho-K combined with visual\u0000 motor training could better correct the vision of juvenile myopia patients and delay ocular axis growth. In addition, the earlier the intervention, the better the effect.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42654746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lung cancer is a common and highly lethal tumor worldwide. Research indicates that NOX4 plays a crucial role in apoptosis resistance and sustained proliferation of cancer cells in various types of tumors. In this study, NOX4 expression in lung cancer tissues was analyzed using the Wilcoxon rank sum test, while the Kruskal-Wallis Test was employed to explore the relationship between NOX4 expression and clinical characteristics in lung cancer patients. Prognostic evaluation was conducted using Kaplan-Meier plotter analysis, Cox regression, and receiver operating characteristic (ROC) curve construction. Gene set enrichment analysis (GSEA) was performed to investigate the correlation between NOX4 and immune infiltration. Results showed significantly higher NOX4 expression in lung cancer patients compared to normal tissues. High NOX4 expression was associated with shorter overall survival (OS) in lung cancer patients, as confirmed by Cox analysis. Furthermore, other clinicopathological factors predicted poor prognosis in lung adenocarcinoma (LUAD), and NOX4 demonstrated diagnostic value according to ROC analysis. Additionally, NOX4 overexpression correlated with macrophages and Th1 cells based on SsGSEA analysis. In summary, NOX4 serves as an independent prognostic biomarker and is associated with immune infiltration in lung cancer.
{"title":"NOX4 Upregulation in Lung Cancer: A Potential Therapeutic Target Associated with Immune Infiltration","authors":"Hong Li, Sifang Feng, Tian Yang, Jiejuan Ning, Yuegang Wu, Tian-jun Chen","doi":"10.1166/jbn.2023.3644","DOIUrl":"https://doi.org/10.1166/jbn.2023.3644","url":null,"abstract":"Lung cancer is a common and highly lethal tumor worldwide. Research indicates that NOX4 plays a crucial role in apoptosis resistance and sustained proliferation of cancer cells in various types of tumors. In this study, NOX4 expression in lung cancer tissues was analyzed using the Wilcoxon\u0000 rank sum test, while the Kruskal-Wallis Test was employed to explore the relationship between NOX4 expression and clinical characteristics in lung cancer patients. Prognostic evaluation was conducted using Kaplan-Meier plotter analysis, Cox regression, and receiver operating characteristic\u0000 (ROC) curve construction. Gene set enrichment analysis (GSEA) was performed to investigate the correlation between NOX4 and immune infiltration. Results showed significantly higher NOX4 expression in lung cancer patients compared to normal tissues. High NOX4 expression was associated with\u0000 shorter overall survival (OS) in lung cancer patients, as confirmed by Cox analysis. Furthermore, other clinicopathological factors predicted poor prognosis in lung adenocarcinoma (LUAD), and NOX4 demonstrated diagnostic value according to ROC analysis. Additionally, NOX4 overexpression correlated\u0000 with macrophages and Th1 cells based on SsGSEA analysis. In summary, NOX4 serves as an independent prognostic biomarker and is associated with immune infiltration in lung cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41746015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigated the role of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in non-small cell lung cancer (NSCLC) and its association with N6-methyladenosine (m6A) modification. The study analyzes the expression levels and stability of IGF2BP3, as well as its impact on NSCLC cell functions. The findings indicate that IGF2BP3 is upregulated in NSCLC patients and cell lines. Knocking down IGF2BP3 reduces cell proliferation and promotes ferroptosis in A549 and H1299 cells. Additionally, the study reveals that IGF2BP3 regulates the m6A modification of the fanconi anemia group D2 protein (FANCD2) and influences its mRNA stability. Overexpressing FANCD2 counteracts the effects of IGF2BP3 silencing and increases the aggressiveness of NSCLC. Furthermore, treatment with celastrol induces ferroptosis in NSCLC cells and inhibits tumor growth in vivo. In conclusion, these findings suggest that IGF2BP3 acts as an oncogene in NSCLC. Its interaction with FANCD2 through m6A modification suppresses ferroptosis in NSCLC cells. Thus, the IGF2BP3/FANCD2 signaling pathway may serve as a potential therapeutic target for NSCLC.
{"title":"Insulin-Like Growth Factor 2 mRNA Binding Protein 3 Suppresses Ferroptosis in Non-Small Cell Lung Cancer via Stabilizing m6A Modification of Fanconi Anemia Group D2 Protein","authors":"Haotian Zhang, Junjie Tang, Xiaowei Gong, Chenjun Huang","doi":"10.1166/jbn.2023.3643","DOIUrl":"https://doi.org/10.1166/jbn.2023.3643","url":null,"abstract":"This study investigated the role of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in non-small cell lung cancer (NSCLC) and its association with N6-methyladenosine (m6A) modification. The study analyzes the expression levels and stability of IGF2BP3, as well as its impact\u0000 on NSCLC cell functions. The findings indicate that IGF2BP3 is upregulated in NSCLC patients and cell lines. Knocking down IGF2BP3 reduces cell proliferation and promotes ferroptosis in A549 and H1299 cells. Additionally, the study reveals that IGF2BP3 regulates the m6A modification of the\u0000 fanconi anemia group D2 protein (FANCD2) and influences its mRNA stability. Overexpressing FANCD2 counteracts the effects of IGF2BP3 silencing and increases the aggressiveness of NSCLC. Furthermore, treatment with celastrol induces ferroptosis in NSCLC cells and inhibits tumor growth in\u0000 vivo. In conclusion, these findings suggest that IGF2BP3 acts as an oncogene in NSCLC. Its interaction with FANCD2 through m6A modification suppresses ferroptosis in NSCLC cells. Thus, the IGF2BP3/FANCD2 signaling pathway may serve as a potential therapeutic target for NSCLC.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49522088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N-Glycosylation is one of the most common and important protein posttranslational modifications. Structural aberrations of the N-glycans branching from glycoproteins are closely related to various disease occurrences and progressions. Therefore, global systematic identification of disease-related N-glycans not only largely facilitates the understanding of their cellular functions but also promotes the development of new diagnostic and therapeutic biomarkers. However, N-glycans are low in abundance and hydrophilic, thereby hindering high-throughput, and large-scale N-glycan analysis and N-glycoproteome research. Here, a zwitterion-functionalized polymer brush-grafted silica hybrid hydrophilic material (poly-SBMA-S) was fabricated by in situ growth of polymeric [2-(methacryloyloxy)ethyl]dimethyl(3-sulfopropyl)ammonium hydroxide (SBMA) chains on porous silica particles through a surface-initiated atom transfer radical polymerization technique, and it was used as a new stationary phase for highly selective N-glycan enrichment. Human plasma demonstrated 64 N-glycans due to the densely packed polyzwitterion chains and the significantly increased coverage of hydrophilic binding sites, demonstrating the high potential of the new hydrophilic matrix in the profiling and analysis of N-glycans and other hydrophilic targets.
{"title":"A Novel Silica Hybrid Nanoparticle with Zwitterion-Functionalized Polymer Chains for Highly Efficient N-Glycan Enrichment","authors":"Yiting Pan, H. Bai, Guocheng Zhang, Wei Liu, Yanqi Wu, Hui Chen, Ying Tian, Zhenqi Yang, Duan Feng, Jiaqi Liu, Dan Wu","doi":"10.1166/jbn.2023.3553","DOIUrl":"https://doi.org/10.1166/jbn.2023.3553","url":null,"abstract":"N-Glycosylation is one of the most common and important protein posttranslational modifications. Structural aberrations of the N-glycans branching from glycoproteins are closely related to various disease occurrences and progressions. Therefore, global systematic identification of disease-related\u0000 N-glycans not only largely facilitates the understanding of their cellular functions but also promotes the development of new diagnostic and therapeutic biomarkers. However, N-glycans are low in abundance and hydrophilic, thereby hindering high-throughput, and large-scale N-glycan analysis\u0000 and N-glycoproteome research. Here, a zwitterion-functionalized polymer brush-grafted silica hybrid hydrophilic material (poly-SBMA-S) was fabricated by in situ growth of polymeric [2-(methacryloyloxy)ethyl]dimethyl(3-sulfopropyl)ammonium hydroxide (SBMA) chains on porous silica particles\u0000 through a surface-initiated atom transfer radical polymerization technique, and it was used as a new stationary phase for highly selective N-glycan enrichment. Human plasma demonstrated 64 N-glycans due to the densely packed polyzwitterion chains and the significantly increased coverage of\u0000 hydrophilic binding sites, demonstrating the high potential of the new hydrophilic matrix in the profiling and analysis of N-glycans and other hydrophilic targets.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48647579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Urologic neoplasm is one of the most general neoplasms, including bladder neoplam, prostate neoplasm and renal neoplasm. Researches have shown that tumor formation is a very complicated process, and it is the result of cell proliferation that is seriously out of control. Long noncoding RNA (lncRNA) and pyroptosis act separately or together on the tumorigenesis, growth and metastasis of urinary tumors, and are related to their treatment and prognosis. In this review, we summarized existing studies and found that lncRNA-mediated pyroptosis can promote or inhibit the development of urinary tumors. Pyroptosis can be achieved by lncRNA-caspase-gasdermin axis. Abnormal lncRNA expression may cause uncontrolled pyroptosis, thus bringing tumor deterioration and adverse prognosis. Controlling pyroptosis of neoplasm cells by regulating pyroptosis-related lncRNAs may be a new entry for the treatment of urinary neoplasms.
{"title":"Crucial Biological Roles of Long Noncoding RNA-Mediated Pyroptosis in Urological Tumors","authors":"Jiaogui Xie, Ying Jiang, Yichao Wang, Dalin He","doi":"10.1166/jbn.2023.3637","DOIUrl":"https://doi.org/10.1166/jbn.2023.3637","url":null,"abstract":"Urologic neoplasm is one of the most general neoplasms, including bladder neoplam, prostate neoplasm and renal neoplasm. Researches have shown that tumor formation is a very complicated process, and it is the result of cell proliferation that is seriously out of control. Long noncoding\u0000 RNA (lncRNA) and pyroptosis act separately or together on the tumorigenesis, growth and metastasis of urinary tumors, and are related to their treatment and prognosis. In this review, we summarized existing studies and found that lncRNA-mediated pyroptosis can promote or inhibit the development\u0000 of urinary tumors. Pyroptosis can be achieved by lncRNA-caspase-gasdermin axis. Abnormal lncRNA expression may cause uncontrolled pyroptosis, thus bringing tumor deterioration and adverse prognosis. Controlling pyroptosis of neoplasm cells by regulating pyroptosis-related lncRNAs may be a\u0000 new entry for the treatment of urinary neoplasms.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48732673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The potential of injectable hydrogels based on natural polysaccharides (NPH) for repairing significant bone defects seems promising. Yet their osteogenic, angiogenic, and mechanical characteristics fall short of expectations. To overcome these disadvantages of chitosan-based hydrogels, silk fibroin-coated magnesium oxide nanoparticles (SF-MgO-NPs) were integrated into fabricated aqueous-soluble phosphocreatine-functionalized chitosan (CMP) solution to create a CMP@SF-MgO-NPs injectable hydrogel via supramolecular assembly. This hydrogel’s phosphocreatine acts as a reservoir to regulate Mg2+ release and as a location for supramolecular interaction with MgO-NPs. Injectable CMP@SF-MgO hydrogels encouraged in vitro calcium phosphate (CaP) deposition, ALP activity, and proliferation to MC3T3-E1 cells. This hydrogel (CMP@SF-MgO-NPs(5)) was also highly effective in stimulating the growth of new bone in calvarial lesions of critical size in rats. Therefore, the injectable hydrogel made of CMP@SF-MgO-NPs shows promising potential for bone regeneration.
{"title":"A Convergent Fabrication of Silk Fibroin Coated Magnesium Oxide Conjugated Phosphate Functionalized Chitosan Hydrogel for Bone Regeneration","authors":"Xi Li, Yong-tao Yi, P. Guo, Ru-dan Zhou, Hua Li","doi":"10.1166/jbn.2023.3652","DOIUrl":"https://doi.org/10.1166/jbn.2023.3652","url":null,"abstract":"The potential of injectable hydrogels based on natural polysaccharides (NPH) for repairing significant bone defects seems promising. Yet their osteogenic, angiogenic, and mechanical characteristics fall short of expectations. To overcome these disadvantages of chitosan-based hydrogels,\u0000 silk fibroin-coated magnesium oxide nanoparticles (SF-MgO-NPs) were integrated into fabricated aqueous-soluble phosphocreatine-functionalized chitosan (CMP) solution to create a CMP@SF-MgO-NPs injectable hydrogel via supramolecular assembly. This hydrogel’s phosphocreatine acts as a\u0000 reservoir to regulate Mg2+ release and as a location for supramolecular interaction with MgO-NPs. Injectable CMP@SF-MgO hydrogels encouraged in vitro calcium phosphate (CaP) deposition, ALP activity, and proliferation to MC3T3-E1 cells. This hydrogel (CMP@SF-MgO-NPs(5)) was also highly\u0000 effective in stimulating the growth of new bone in calvarial lesions of critical size in rats. Therefore, the injectable hydrogel made of CMP@SF-MgO-NPs shows promising potential for bone regeneration.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45802880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingying Kong, Periyannan Velu, A. Vijayalakshmi, Chao Yuan
This study investigated green synthesis of magnetic nanoparticles (MNP) using Oldenlandia diffusa, its cauterization, and therapeutic propertieson liver cancer cells via an in-vitro approach. Anti-cancer, anti-oxidant, and anti-diabetic properties of MNP were evaluated. Synthesis of MNP was confirmed via UV-visible spectroscopy. Oldenlandia diffusa MNP (20–40 nm) showed absorption peak at 327 nm in UV-visible, XRD, EDX and FTIR spectral analysis. MNP were monoclinic, and rhombohedral with two stretching peaks at 646 cm−1, and 1098 cm−1 confirming Fe–O–Fe mode and Fe = O due to the iron element. IC50 values for MNP (IC50 74 μg/ml & 58 μg/ml) using DPPH and ABTS methods were high as compared to aqueous leaf extract of Oldenlandia diffusa confirming anti-oxidant properties. MNP exhibited α-amylase inhibitory activity with maximum inhibition of 82.17±0.82 μg/ml for α-glucosidase inhibitory action of 84.82±0.89 μg/ml. MNP showed dose-dependant in-vitro tumor suppression activity against HepG2 cells (16.08% at 150 μg/ml and 28.31% at 150 μg/ml). MNP also demonstrated anti-leishmanial activity at 400 g/ml (74.26±0.82 g/ml for promastigotes and 69.40±0.69 μg/ml for amastigotes). Oldenlandia diffusa actively bonded with magnetic Fe-nanoparticles and suppressed the growth of HepG2 cells. It demonstrated anti-cancer, antioxidant, anti-leishmanaland anti-diabetic activities. Thus, Oldenlandia diffusa is a potential anti-cancer therapeutic drug for liver cancer.
{"title":"Green Synthesis of Magnetic Nanoparticles from Oldenlandia diffusa: Characterization and Therapeutic Activities","authors":"Yingying Kong, Periyannan Velu, A. Vijayalakshmi, Chao Yuan","doi":"10.1166/jbn.2023.3660","DOIUrl":"https://doi.org/10.1166/jbn.2023.3660","url":null,"abstract":"This study investigated green synthesis of magnetic nanoparticles (MNP) using Oldenlandia diffusa, its cauterization, and therapeutic propertieson liver cancer cells via an in-vitro approach. Anti-cancer, anti-oxidant, and anti-diabetic properties of MNP were evaluated.\u0000 Synthesis of MNP was confirmed via UV-visible spectroscopy. Oldenlandia diffusa MNP (20–40 nm) showed absorption peak at 327 nm in UV-visible, XRD, EDX and FTIR spectral analysis. MNP were monoclinic, and rhombohedral with two stretching peaks at 646 cm−1, and\u0000 1098 cm−1 confirming Fe–O–Fe mode and Fe = O due to the iron element. IC50 values for MNP (IC50 74 μg/ml & 58 μg/ml) using DPPH and ABTS methods were high as compared to aqueous leaf extract of Oldenlandia diffusa\u0000 confirming anti-oxidant properties. MNP exhibited α-amylase inhibitory activity with maximum inhibition of 82.17±0.82 μg/ml for α-glucosidase inhibitory action of 84.82±0.89 μg/ml. MNP showed dose-dependant in-vitro tumor suppression\u0000 activity against HepG2 cells (16.08% at 150 μg/ml and 28.31% at 150 μg/ml). MNP also demonstrated anti-leishmanial activity at 400 g/ml (74.26±0.82 g/ml for promastigotes and 69.40±0.69 μg/ml for amastigotes). Oldenlandia diffusa actively\u0000 bonded with magnetic Fe-nanoparticles and suppressed the growth of HepG2 cells. It demonstrated anti-cancer, antioxidant, anti-leishmanaland anti-diabetic activities. Thus, Oldenlandia diffusa is a potential anti-cancer therapeutic drug for liver cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47387858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigated the association between HOXD9 expression and prognosis in non-small cell lung cancer (NSCLC) and explored its underlying mechanisms in tumor progression. HOXD9 expression was examined in 42 pairs of NSCLC tissue samples using qRT-PCR, and its effects were evaluated in NSCLC cell lines through lentiviral vector transfection, assessing cell growth and migration using CCK-8 and Transwell assays. Bioinformatics analysis identified KLK9 as a target gene of HOXD9, and their interaction was investigated in vitro and in vivo. HOXD9 mRNA expression was significantly higher in NSCLC tissues compared to adjacent tissues, positively correlating with metastasis incidence and poor prognosis. HOXD9 knockdown reduced NSCLC cell migration and proliferation, while overexpression had the opposite effect. Luciferase assays confirmed HOXD9′s binding to KLK9, leading to negative regulation of KLK9 expression. Knockdown of KLK9 reversed the inhibitory effect of HOXD9 on NSCLC cell migration and proliferation, while co-overexpression of KLK9 and HOXD9 had the opposite effect. Our findings demonstrate the close association of high HOXD9 expression in NSCLC tissues with metastasis incidence and poor prognosis. HOXD9 likely exerts its biological function in NSCLC by negatively regulating KLK9. These results provide valuable insights into the molecular mechanisms driving NSCLC progression, highlighting HOXD9 as a potential prognostic biomarker and therapeutic target.
{"title":"Homeobox D9 Promotes Malignant Progression of Non-Small Cell Lung Cancer Through Negatively Regulating Kallikrein Related Peptidase 9","authors":"Juntao Hao, Zengqiang Shen, Junjun Ma","doi":"10.1166/jbn.2023.3664","DOIUrl":"https://doi.org/10.1166/jbn.2023.3664","url":null,"abstract":"We investigated the association between HOXD9 expression and prognosis in non-small cell lung cancer (NSCLC) and explored its underlying mechanisms in tumor progression. HOXD9 expression was examined in 42 pairs of NSCLC tissue samples using qRT-PCR, and its effects were evaluated in\u0000 NSCLC cell lines through lentiviral vector transfection, assessing cell growth and migration using CCK-8 and Transwell assays. Bioinformatics analysis identified KLK9 as a target gene of HOXD9, and their interaction was investigated in vitro and in vivo. HOXD9 mRNA expression\u0000 was significantly higher in NSCLC tissues compared to adjacent tissues, positively correlating with metastasis incidence and poor prognosis. HOXD9 knockdown reduced NSCLC cell migration and proliferation, while overexpression had the opposite effect. Luciferase assays confirmed HOXD9′s\u0000 binding to KLK9, leading to negative regulation of KLK9 expression. Knockdown of KLK9 reversed the inhibitory effect of HOXD9 on NSCLC cell migration and proliferation, while co-overexpression of KLK9 and HOXD9 had the opposite effect. Our findings demonstrate the close association of high\u0000 HOXD9 expression in NSCLC tissues with metastasis incidence and poor prognosis. HOXD9 likely exerts its biological function in NSCLC by negatively regulating KLK9. These results provide valuable insights into the molecular mechanisms driving NSCLC progression, highlighting HOXD9 as a potential\u0000 prognostic biomarker and therapeutic target.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47593813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huiying Zuo, Hui Fang Xiao, Deqiang Wang, Ranran Wang
The establishment of a tumor theranostic platform with high efficacy is attracting considerable interest in cancer treatment. Nevertheless, considerable obstacles need to be overcome in developing such a platform. In this study, a versatile theranostic system (CM-FMNPs-Cur) was produced utilizing MnO2 nanoflowers, which provided targeted and synergistic therapy for cancer through the incorporation of sonodynamic and chemodynamic therapies. By integrating Fe3O4, MnO2, and curcumin, CM-FMNPs-Cur manifests a substantial amount of reactive oxygen species toward deep-seated cancers. As a sonosensitizer, curcumin was used for chemodynamic therapy (CDT)_and sonodynamic therapy (SDT). In the acidic environment of malignant cells, CM-FMNPs-Cur can decompose into Mn2+ and Fe2+, triggering a Fenton-like reaction and generating ·OH radicals and oxygen. Ameliorating tumor hypoxia also enhanced the effectiveness of SDT in neoplasms. The homologous targeting effect of CM-FMNPs-Cur on cancer cell membranes led to its accumulation in the tumor region. Here, the mutual promotion of CDT and SDT resulted in an amplified therapeutic outcome. All these results revealed the vast potential of CM-FMNPs-Cur for secure and highly effective collaborative tumor treatment, indicating future clinical applications.
{"title":"Ultrasound-Promoted Nanoreagent for Homologous Targeted Synergistic Chemodynamic/Sonodynamic Tumor Therapy","authors":"Huiying Zuo, Hui Fang Xiao, Deqiang Wang, Ranran Wang","doi":"10.1166/jbn.2023.3655","DOIUrl":"https://doi.org/10.1166/jbn.2023.3655","url":null,"abstract":"The establishment of a tumor theranostic platform with high efficacy is attracting considerable interest in cancer treatment. Nevertheless, considerable obstacles need to be overcome in developing such a platform. In this study, a versatile theranostic system (CM-FMNPs-Cur) was produced\u0000 utilizing MnO2 nanoflowers, which provided targeted and synergistic therapy for cancer through the incorporation of sonodynamic and chemodynamic therapies. By integrating Fe3O4, MnO2, and curcumin, CM-FMNPs-Cur manifests a substantial amount of reactive\u0000 oxygen species toward deep-seated cancers. As a sonosensitizer, curcumin was used for chemodynamic therapy (CDT)_and sonodynamic therapy (SDT). In the acidic environment of malignant cells, CM-FMNPs-Cur can decompose into Mn2+ and Fe2+, triggering a Fenton-like reaction\u0000 and generating ·OH radicals and oxygen. Ameliorating tumor hypoxia also enhanced the effectiveness of SDT in neoplasms. The homologous targeting effect of CM-FMNPs-Cur on cancer cell membranes led to its accumulation in the tumor region. Here, the mutual promotion of CDT and SDT resulted\u0000 in an amplified therapeutic outcome. All these results revealed the vast potential of CM-FMNPs-Cur for secure and highly effective collaborative tumor treatment, indicating future clinical applications.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48859292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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