Arjun Patra, Swaha Satpathy, Pradeep K. Naik, Mohsin Kazi, Muhammad Hussain Delwar
Cancer is a dreadful disease with a high mortality rate and breast cancer is the most common cancer among females in the world. Different strategies have been used for the treatment of breast cancer, including chemotherapy but it has a wide range of side effects. This problem can be overcome by delivering anticancer agents with nano-formulations. Apigenin (4′,5,7-trihydroxyflavone), present in many different medicinal plants, shows potential anticancer properties in various cancers. However, its use in clinical practice is limited due to its low water solubility and bioavailability. In this study, we examined folate receptor-targeted and PEGylated poly(lactide-co-glycolide) nanoparticles (PLGA-PEG-FA NPs) containing apigenin for targeted delivery to MCF-7 breast cancer cells. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs were small in size, had a negative zeta potential, showed sustained release of apigenin and showed significantly higher anticancer activity than the free drug in breast cancer cells. The half maximal inhibitory concentration (IC50) values of apigenin, apigenin-loaded PLGA, PLGA-PEG and PLGA-PEG-FA NPs were 50.2, 49.4, 18.1 and 13.3 μM, respectively. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs showed 2.79- and 3.77-fold higher cytotoxicity than the pristine drug. Folate-conjugated PLGA nanoparticles could be developed for potential target-specific delivery of apigenin in the treatment of breast cancer.
{"title":"Folate Receptor-Targeted Nanodelivery of Apigenin in Breast Cancer: Formulation Development, Characterization and In Vitro Evaluation","authors":"Arjun Patra, Swaha Satpathy, Pradeep K. Naik, Mohsin Kazi, Muhammad Hussain Delwar","doi":"10.1166/jbn.2024.3803","DOIUrl":"https://doi.org/10.1166/jbn.2024.3803","url":null,"abstract":"Cancer is a dreadful disease with a high mortality rate and breast cancer is the most common cancer among females in the world. Different strategies have been used for the treatment of breast cancer, including chemotherapy but it has a wide range of side effects. This problem can be\u0000 overcome by delivering anticancer agents with nano-formulations. Apigenin (4′,5,7-trihydroxyflavone), present in many different medicinal plants, shows potential anticancer properties in various cancers. However, its use in clinical practice is limited due to its low water solubility\u0000 and bioavailability. In this study, we examined folate receptor-targeted and PEGylated poly(lactide-co-glycolide) nanoparticles (PLGA-PEG-FA NPs) containing apigenin for targeted delivery to MCF-7 breast cancer cells. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs were small in size, had a negative\u0000 zeta potential, showed sustained release of apigenin and showed significantly higher anticancer activity than the free drug in breast cancer cells. The half maximal inhibitory concentration (IC50) values of apigenin, apigenin-loaded PLGA, PLGA-PEG and PLGA-PEG-FA NPs were 50.2,\u0000 49.4, 18.1 and 13.3 μM, respectively. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs showed 2.79- and 3.77-fold higher cytotoxicity than the pristine drug. Folate-conjugated PLGA nanoparticles could be developed for potential target-specific delivery of apigenin in the treatment of\u0000 breast cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140355789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Physical stimulation plays a crucial role in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs). However, the mechanotransductive mechanisms remain uncleared. Recent studies have suggested that the Piezo1 channel is essential for transforming mechanical signals. Therefore, we investigate the Piezo1-mediated mechanisms in mechanical strain-regulated MSC osteogenic differentiation and release of proinflammatory cytokines. The tensile strain was applied to rat MSCs cultured in a monolayer to induce mechanical strain. The immuno-nanomagnetic bead enzyme-linked immunosorbent assay was employed to assess gene and protein expressions, as well as osteogenic biomarkers and interleukin-6 (IL-6) release, both in the presence or absence of a Piezo1 agonist/antagonist. Firstly, biophysical loading through mechanical strain was found to promote MSC osteogenic differentiation. Suppression of Piezo1 using GsMTx4 antagonist or transfection with Piezo1-siRNA effectively inhibited mechanical responses associated with osteogenic gene expressions and IL-6. Activation of Piezo1 by Yoda1 mimicked the effects induced by mechanical strain on osteogenic gene expressions and IL-6 release, which were associated with YAP activation, upregulation, and nuclear accumulation of β-catenin. In conclusion, these findings significantly enhance our understanding of MSC mechanotransduction and hold great promise for drug development to enhance skeletal mass.
{"title":"Effect of Piezo1 Channel-Mediated Mechanotransduction on Osteogenic Differentiation and Interleukin-6 Secretion in Bone Mesenchymal Stem Cells Under Tensile Strain","authors":"Xu Yan, Su Fu, Ying Xie, Xuejian Wu","doi":"10.1166/jbn.2024.3730","DOIUrl":"https://doi.org/10.1166/jbn.2024.3730","url":null,"abstract":"Physical stimulation plays a crucial role in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs). However, the mechanotransductive mechanisms remain uncleared. Recent studies have suggested that the Piezo1 channel is essential for transforming mechanical\u0000 signals. Therefore, we investigate the Piezo1-mediated mechanisms in mechanical strain-regulated MSC osteogenic differentiation and release of proinflammatory cytokines. The tensile strain was applied to rat MSCs cultured in a monolayer to induce mechanical strain. The immuno-nanomagnetic\u0000 bead enzyme-linked immunosorbent assay was employed to assess gene and protein expressions, as well as osteogenic biomarkers and interleukin-6 (IL-6) release, both in the presence or absence of a Piezo1 agonist/antagonist. Firstly, biophysical loading through mechanical strain was found to\u0000 promote MSC osteogenic differentiation. Suppression of Piezo1 using GsMTx4 antagonist or transfection with Piezo1-siRNA effectively inhibited mechanical responses associated with osteogenic gene expressions and IL-6. Activation of Piezo1 by Yoda1 mimicked the effects induced by mechanical\u0000 strain on osteogenic gene expressions and IL-6 release, which were associated with YAP activation, upregulation, and nuclear accumulation of β-catenin. In conclusion, these findings significantly enhance our understanding of MSC mechanotransduction and hold great promise for drug\u0000 development to enhance skeletal mass.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinglin Wu, Yang Jiao, Jieshi Wu, Sujiajun Zhang, Ruisheng Xu, Quanming Zhao, K. Lu, Pengpeng Zhang, Lu Zhang, Xiaohui Ni
Titanium and titanium alloys are receiving widespread attention due to their excellent comprehensive mechanical properties, corrosion resistances, and biocompatibilities. However, titanium metal itself is biologically inert in physiological environments, and after implantation, it is surrounded by a layer of encapsulating fibrous membrane, making it difficult to form solid bonds with the tissue. Plasma electrolytic oxidation is a new technology used to prepare bioactive porous ceramic membranes on the surfaces of titanium and titanium alloys. It has application prospects for biomimetic surface modifications of titanium alloys. In this study, a cobalt, strontium and fluorine codoped oxide film (TAM-CSF) was prepared on a titanium surface via plasma electrolytic oxidation. The surface characteristics of the film were evaluated with field emission scanning electron microscopy, energy spectrum analyses, atomic force microscopy, profilometry and X-ray photoelectron spectroscopy. Additionally, the corrosion performance of the material was evaluated with an electrochemical workstation. The biocompatibility and bioactivity of the film were tested with in vitro cell experiments. The results showed that the TAM-CSF on the titanium surface had a porous morphology, and the CSF was uniformly doped on the film surface. TAM-CSF improved the surface roughness of the titanium. This film exhibited good biocompatibility and promoted the extension and proliferation of MC3T3-E1 cells. It was possible to prepare TAM-CSF on titanium surfaces via plasma electrolytic oxidation. The film exhibited a good morphology and good biological activity and has clinical application prospects.
钛和钛合金因其优异的综合机械性能、耐腐蚀性和生物相容性而受到广泛关注。然而，钛金属本身在生理环境中具有生物惰性，植入人体后被一层包裹性纤维膜包围，很难与组织形成牢固的结合。等离子电解氧化是一种在钛和钛合金表面制备生物活性多孔陶瓷膜的新技术。它在钛合金的仿生物表面改性方面具有应用前景。本研究通过等离子电解氧化法在钛表面制备了钴锶氟共掺氧化物薄膜（TAM-CSF）。利用场发射扫描电子显微镜、能谱分析、原子力显微镜、轮廓仪和 X 射线光电子能谱对薄膜的表面特性进行了评估。此外，还利用电化学工作站对材料的腐蚀性能进行了评估。体外细胞实验测试了薄膜的生物相容性和生物活性。结果表明，钛表面的 TAM-CSF 呈多孔状，CSF 均匀地掺杂在薄膜表面。TAM-CSF 改善了钛的表面粗糙度。这种薄膜具有良好的生物相容性，能促进 MC3T3-E1 细胞的扩展和增殖。通过等离子电解氧化法可以在钛表面制备 TAM-CSF。该薄膜具有良好的形态和生物活性，具有临床应用前景。
{"title":"Preparation, Characterization, and Bioactivities of Cobalt, Strontium and Fluorine Co-Doped Oxide Films on Titanium Surface for Clinical Application","authors":"Xinglin Wu, Yang Jiao, Jieshi Wu, Sujiajun Zhang, Ruisheng Xu, Quanming Zhao, K. Lu, Pengpeng Zhang, Lu Zhang, Xiaohui Ni","doi":"10.1166/jbn.2024.3813","DOIUrl":"https://doi.org/10.1166/jbn.2024.3813","url":null,"abstract":"Titanium and titanium alloys are receiving widespread attention due to their excellent comprehensive mechanical properties, corrosion resistances, and biocompatibilities. However, titanium metal itself is biologically inert in physiological environments, and after implantation, it is\u0000 surrounded by a layer of encapsulating fibrous membrane, making it difficult to form solid bonds with the tissue. Plasma electrolytic oxidation is a new technology used to prepare bioactive porous ceramic membranes on the surfaces of titanium and titanium alloys. It has application prospects\u0000 for biomimetic surface modifications of titanium alloys. In this study, a cobalt, strontium and fluorine codoped oxide film (TAM-CSF) was prepared on a titanium surface via plasma electrolytic oxidation. The surface characteristics of the film were evaluated with field emission scanning electron\u0000 microscopy, energy spectrum analyses, atomic force microscopy, profilometry and X-ray photoelectron spectroscopy. Additionally, the corrosion performance of the material was evaluated with an electrochemical workstation. The biocompatibility and bioactivity of the film were tested with in\u0000 vitro cell experiments. The results showed that the TAM-CSF on the titanium surface had a porous morphology, and the CSF was uniformly doped on the film surface. TAM-CSF improved the surface roughness of the titanium. This film exhibited good biocompatibility and promoted the extension\u0000 and proliferation of MC3T3-E1 cells. It was possible to prepare TAM-CSF on titanium surfaces via plasma electrolytic oxidation. The film exhibited a good morphology and good biological activity and has clinical application prospects.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigates the role of the long non-coding RNA EGOT in colorectal cancer (CRC) by examining its expression in 40 pairs of CRC and adjacent normal tissues and assessing its impact on clinical outcomes. EGOT was found to be downregulated in CRC tissues, and low EGOT levels were associated with a higher likelihood of lymphatic and distant metastasis, as well as poorer overall and progression-free survival in CRC patients. Functional experiments revealed that overexpression of EGOT in SW480 cells reduced cell viability, migration, and wound closure, while knockdown of EGOT in LoVo cells had the opposite effect. In vivo experiments with nude mice confirmed that EGOT downregulation accelerated CRC growth, whereas its overexpression slowed tumor growth. The study identified BTG3 as the target gene of EGOT, and they exhibited a negative correlation in CRC tissues. Rescue experiments demonstrated that BTG3 could reverse the effects of EGOT on CRC cell phenotypes. In conclusion, EGOT is a downregulated molecule in CRC, closely associated with metastasis and patient prognosis. It exerts a suppressive influence on CRC cell proliferation, migration, and tumorigenesis by negatively regulating BTG3.
{"title":"Long-Noncoding RNA (lncRNA) EGOT Prevents the Malignant Process of Colorectal Carcinoma by Regulating BTG3","authors":"Zhengdong Wang, Dezhi Gu, Aiming Zhou","doi":"10.1166/jbn.2024.3823","DOIUrl":"https://doi.org/10.1166/jbn.2024.3823","url":null,"abstract":"This study investigates the role of the long non-coding RNA EGOT in colorectal cancer (CRC) by examining its expression in 40 pairs of CRC and adjacent normal tissues and assessing its impact on clinical outcomes. EGOT was found to be downregulated in CRC tissues, and low EGOT levels\u0000 were associated with a higher likelihood of lymphatic and distant metastasis, as well as poorer overall and progression-free survival in CRC patients. Functional experiments revealed that overexpression of EGOT in SW480 cells reduced cell viability, migration, and wound closure, while knockdown\u0000 of EGOT in LoVo cells had the opposite effect. In vivo experiments with nude mice confirmed that EGOT downregulation accelerated CRC growth, whereas its overexpression slowed tumor growth. The study identified BTG3 as the target gene of EGOT, and they exhibited a negative correlation\u0000 in CRC tissues. Rescue experiments demonstrated that BTG3 could reverse the effects of EGOT on CRC cell phenotypes. In conclusion, EGOT is a downregulated molecule in CRC, closely associated with metastasis and patient prognosis. It exerts a suppressive influence on CRC cell proliferation,\u0000 migration, and tumorigenesis by negatively regulating BTG3.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140352737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhaodi Liu, Yang Liu, Xinyue Wu, Xiangnan Feng, Wei Liang
This study screened important genes contributing to morbidity from differential ferroptosis-related genes (FRGs) in cervical cancer and to establish a risk assessment model with ferroptosis-related LncRNAs. Total RNA sequencing data were extracted from The cancer genome atlas (TCGA), Gene Expression Omnibus (GEO) and Genotype-Tissue Expression (GTEx). By differential analysis, we identified ferroptosis-related hub genes close to prevalence of cervical cancer. According to receiver operator curves (ROC) curves, hub genes have good diagnostic performance. The diagnostic potential of hub genes for occurrence of the disease was further assessed and verified. Further, a risk-assessing model based on ferroptosis-related LncRNAs was established. Finally, the differential expressions of hub genes were verified through qRT-PCR. Five hub genes were identified, and we found through GO, KEGG and immune infiltration, that the hub genes are connection with cervical cancer. The Area Under Curve (AUC) values were all greater than 0.8 in ROC curve, and the hub genes presented differences between disease and control groups in validation set GSE29570. We created a risk assessment model with 16 ferroptosis-related LncRNAs. There was a difference in survival between high-risk and low-risk groups. The AUC result for risk assessment model reached 0.792, and there were significant expression differences of Hub genes in Huvec and Hela cells. The study screened 5 hub genes and constructed the risk-assessment model based on 16 LncRNAs associated with ferroptosis genes.
{"title":"Identification and Verification of Potential Ferroptosis-Related Biomarkers in Cervical Cancer","authors":"Zhaodi Liu, Yang Liu, Xinyue Wu, Xiangnan Feng, Wei Liang","doi":"10.1166/jbn.2024.3810","DOIUrl":"https://doi.org/10.1166/jbn.2024.3810","url":null,"abstract":"This study screened important genes contributing to morbidity from differential ferroptosis-related genes (FRGs) in cervical cancer and to establish a risk assessment model with ferroptosis-related LncRNAs. Total RNA sequencing data were extracted from The cancer genome atlas (TCGA),\u0000 Gene Expression Omnibus (GEO) and Genotype-Tissue Expression (GTEx). By differential analysis, we identified ferroptosis-related hub genes close to prevalence of cervical cancer. According to receiver operator curves (ROC) curves, hub genes have good diagnostic performance. The diagnostic\u0000 potential of hub genes for occurrence of the disease was further assessed and verified. Further, a risk-assessing model based on ferroptosis-related LncRNAs was established. Finally, the differential expressions of hub genes were verified through qRT-PCR. Five hub genes were identified, and\u0000 we found through GO, KEGG and immune infiltration, that the hub genes are connection with cervical cancer. The Area Under Curve (AUC) values were all greater than 0.8 in ROC curve, and the hub genes presented differences between disease and control groups in validation set GSE29570. We created\u0000 a risk assessment model with 16 ferroptosis-related LncRNAs. There was a difference in survival between high-risk and low-risk groups. The AUC result for risk assessment model reached 0.792, and there were significant expression differences of Hub genes in Huvec and Hela cells. The study screened\u0000 5 hub genes and constructed the risk-assessment model based on 16 LncRNAs associated with ferroptosis genes.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140355903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the role of interleukin-37 (IL-37) expression in lung tissues of sepsis-induced acute lung injury (ALI) rats and its impact on ALI, along with the underlying mechanisms. Sprague-Dawley (SD) rats were categorized into three groups: Control, sepsis-induced ALI (via cecal ligation and puncture, CLP), and sepsis-induced ALI with antibiotics (CLP+An). ALI models were established, and lung tissue injuries were assessed through hematoxylineosin staining. mRNA levels of IL-1α, IL-1β, IL-37, and tumor necrosis factor-α (TNF-α) were measured via RT-PCR, while IL-37 protein levels in lung tissues were determined using Western blotting. Additionally, bronchoalveolar lavage fluid (BALF) and blood samples were collected to assess inflammatory factors through ELISA. In the CLP group, there was an increase in pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in lung tissues and serum. However, in the CLP+An group, these factors decreased, IL-37 expression increased, and oxidative stress levels decreased. IL-37 demonstrated an inhibitory effect on the release of pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in sepsis rats, leading to a reduction in lung tissue inflammation. Furthermore, IL-37 exhibited a protective role by reducing oxidative stress in sepsis-induced lung tissues. These findings highlight IL-37 as a potential therapeutic target for mitigating ALI in sepsis.
本研究旨在探讨白细胞介素-37（IL-37）在脓毒症诱导的急性肺损伤（ALI）大鼠肺组织中的表达作用及其对ALI的影响和潜在机制。斯普拉格-道利（SD）大鼠分为三组：对照组、脓毒症诱导的 ALI 组（通过盲肠结扎和穿刺，CLP）和脓毒症诱导的 ALI 加抗生素组（CLP+An）。通过 RT-PCR 测定 IL-1α、IL-1β、IL-37 和肿瘤坏死因子-α（TNF-α）的 mRNA 水平，通过 Western 印迹测定肺组织中 IL-37 蛋白水平。此外，还收集了支气管肺泡灌洗液（BALF）和血液样本，通过酶联免疫吸附法评估炎症因子。在CLP组中，肺组织和血清中的促炎因子（IL-1α、IL-1β和TNF-α）有所增加。但在 CLP+An 组，这些因子减少，IL-37 表达增加，氧化应激水平降低。IL-37 对败血症大鼠促炎因子（IL-1α、IL-1β 和 TNF-α）的释放具有抑制作用，从而减轻了肺组织炎症。此外，IL-37 还能降低败血症诱导的肺组织中的氧化应激，从而起到保护作用。这些发现突出表明，IL-37 是减轻脓毒症 ALI 的潜在治疗靶点。
{"title":"Associations of Inflammation, Oxidative Stress and Prognosis with IL-37 Expression in Sepsis Rats with Lung Injury","authors":"Fei Hong, Jungang Zhao, Mei Yang, Qian Liu, Qin Chen, Feng Liu, Guoji Zhu","doi":"10.1166/jbn.2024.3822","DOIUrl":"https://doi.org/10.1166/jbn.2024.3822","url":null,"abstract":"This study aimed to investigate the role of interleukin-37 (IL-37) expression in lung tissues of sepsis-induced acute lung injury (ALI) rats and its impact on ALI, along with the underlying mechanisms. Sprague-Dawley (SD) rats were categorized into three groups: Control, sepsis-induced\u0000 ALI (via cecal ligation and puncture, CLP), and sepsis-induced ALI with antibiotics (CLP+An). ALI models were established, and lung tissue injuries were assessed through hematoxylineosin staining. mRNA levels of IL-1α, IL-1β, IL-37, and tumor necrosis factor-α\u0000 (TNF-α) were measured via RT-PCR, while IL-37 protein levels in lung tissues were determined using Western blotting. Additionally, bronchoalveolar lavage fluid (BALF) and blood samples were collected to assess inflammatory factors through ELISA. In the CLP group, there was an\u0000 increase in pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in lung tissues and serum. However, in the CLP+An group, these factors decreased, IL-37 expression increased, and oxidative stress levels decreased. IL-37 demonstrated an inhibitory effect\u0000 on the release of pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in sepsis rats, leading to a reduction in lung tissue inflammation. Furthermore, IL-37 exhibited a protective role by reducing oxidative stress in sepsis-induced lung tissues. These findings\u0000 highlight IL-37 as a potential therapeutic target for mitigating ALI in sepsis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang
In this study, the impact of micro ribonucleic acid (miR)-92a on rats with myocardial ischemia-reperfusion injury was investigated, with a focus on its regulation of the Wnt/β-catenin pathway. A total of 36 Sprague Dawley rats were divided into three groups: a sham operation group, a model group, and a miR-92a antagomir group. The sham group underwent thoracotomy without injury, while the model and miR-92a antagomir groups were subjected to myocardial ischemiareperfusion injury and treated with saline and miR-92a antagomir, respectively. Results showed that the myocardial infarction area was significantly reduced in the miR-92a antagomir group compared to the model group. Histological analysis revealed improved myocardial tissue structure in the miR-92a antagomir group. Western blotting demonstrated elevated levels of p-GSK-3β and β-catenin in both the model and miR-92a antagomir groups, with a notable decrease in the miR-92a antagomir group compared to the model group. Additionally, miR-92a expression was higher in both the model and miR-92a antagomir groups compared to the sham group. Lastly, apoptosis rates were increased in both the model and miR-92a antagomir groups, but significantly reduced in the miR-92a antagomir group compared to the model group. Overall, these findings suggest that miR-92a exacerbates apoptosis in rats with myocardial ischemia-reperfusion injury by up-regulating the Wnt/β-catenin signaling pathway.
{"title":"MiR-92a Promotes Apoptosis in Rats with Myocardial Ischemia-Reperfusion Injury via Regulating Wnt/β-Catenin Pathway","authors":"Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang","doi":"10.1166/jbn.2024.3818","DOIUrl":"https://doi.org/10.1166/jbn.2024.3818","url":null,"abstract":"In this study, the impact of micro ribonucleic acid (miR)-92a on rats with myocardial ischemia-reperfusion injury was investigated, with a focus on its regulation of the Wnt/β-catenin pathway. A total of 36 Sprague Dawley rats were divided into three groups: a sham operation\u0000 group, a model group, and a miR-92a antagomir group. The sham group underwent thoracotomy without injury, while the model and miR-92a antagomir groups were subjected to myocardial ischemiareperfusion injury and treated with saline and miR-92a antagomir, respectively. Results showed that the\u0000 myocardial infarction area was significantly reduced in the miR-92a antagomir group compared to the model group. Histological analysis revealed improved myocardial tissue structure in the miR-92a antagomir group. Western blotting demonstrated elevated levels of p-GSK-3β and β-catenin\u0000 in both the model and miR-92a antagomir groups, with a notable decrease in the miR-92a antagomir group compared to the model group. Additionally, miR-92a expression was higher in both the model and miR-92a antagomir groups compared to the sham group. Lastly, apoptosis rates were increased\u0000 in both the model and miR-92a antagomir groups, but significantly reduced in the miR-92a antagomir group compared to the model group. Overall, these findings suggest that miR-92a exacerbates apoptosis in rats with myocardial ischemia-reperfusion injury by up-regulating the Wnt/β-catenin\u0000 signaling pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140354169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study investigate the changes of white matter injury induced by radiation after radiotherapy in patients with nasopharyngeal carcinoma (NPC) and its association with cognitive dysfunction using multiple MRI methods. A total of 42 patients with NPC who underwent radiotherapy at xxx Hospital between December 2018 and June 2021 were included. The patients were randomly divided into 4 groups based on the timing of radiotherapy. Superparamagnetic iron oxide nanoparticles were used as MRI contrast agents. DTI and MRS scans were conducted to measure FA, ADC, NAA/Cho, Cho/Cr, and NAA/Cr ratios in the hippocampus of both temporal lobes. A cognitive assessment was performed using the MoCA and MMSE scales. After radiotherapy, patients experienced a decline in cognitive scores, which stabilized after 6 months. White matter changes were observed in the hippocampus, with decreased FA and increased ADC values that gradually returned to normal levels. Cho value increased and NAA value decreased initially but eventually returned to pre-treatment levels. No significant changes occurred in the Cr value. Metabolite ratios decreased within 3 months post-radiotherapy but gradually increased thereafter, remaining lower than pre-treatment levels at 6 months. Higher radiation doses did not significantly affect FA and ADC values but decreased white matter metabolite ratios. In conclusion, we reveal that the dosage and duration of radiotherapy can influence the degree of brain injury in patients with NPC and highlights the cognitive decline, white matter changes, and changes in metabolite ratios after radiotherapy for NPC, providing insights into the effects of radiation on brain structure and function.
{"title":"Impact of Radiotherapy on Radiation-Induced Brain Injury in Patients with Nasopharyngeal Carcinoma Using Nano-Magnetic Resonance Imaging","authors":"Baomin Zheng, Hanyong Zhang, Weihu Wang","doi":"10.1166/jbn.2024.3809","DOIUrl":"https://doi.org/10.1166/jbn.2024.3809","url":null,"abstract":"This study investigate the changes of white matter injury induced by radiation after radiotherapy in patients with nasopharyngeal carcinoma (NPC) and its association with cognitive dysfunction using multiple MRI methods. A total of 42 patients with NPC who underwent radiotherapy at\u0000 xxx Hospital between December 2018 and June 2021 were included. The patients were randomly divided into 4 groups based on the timing of radiotherapy. Superparamagnetic iron oxide nanoparticles were used as MRI contrast agents. DTI and MRS scans were conducted to measure FA, ADC, NAA/Cho, Cho/Cr,\u0000 and NAA/Cr ratios in the hippocampus of both temporal lobes. A cognitive assessment was performed using the MoCA and MMSE scales. After radiotherapy, patients experienced a decline in cognitive scores, which stabilized after 6 months. White matter changes were observed in the hippocampus,\u0000 with decreased FA and increased ADC values that gradually returned to normal levels. Cho value increased and NAA value decreased initially but eventually returned to pre-treatment levels. No significant changes occurred in the Cr value. Metabolite ratios decreased within 3 months post-radiotherapy\u0000 but gradually increased thereafter, remaining lower than pre-treatment levels at 6 months. Higher radiation doses did not significantly affect FA and ADC values but decreased white matter metabolite ratios. In conclusion, we reveal that the dosage and duration of radiotherapy can influence\u0000 the degree of brain injury in patients with NPC and highlights the cognitive decline, white matter changes, and changes in metabolite ratios after radiotherapy for NPC, providing insights into the effects of radiation on brain structure and function.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140357298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
miR-302b and DKK1 are two molecules related to the regulation of bone metabolism. Mesoporous silica is a potential drug carrier. This article aims to study the mechanism of mesoporous silica carrying miR-302b targeting DKK1 regulation to improve bone homeostasis imbalance in osteoporotic fractures. Mesoporous silica nanoparticles were synthesized and characterized. miR-302b was loaded into mesoporous silica to form composite nanoparticles. In vivo rat model experiments were performed to evaluate bone metabolism. X-ray examination and μCT scan were used to detect the bone content and trabecular bone status of rats. Alcian blue/hematoxylin/Orange G staining was used to observe changes in trabecular bone in the tibial metaphysis. Immunohistochemical staining showed the formation of trabecular bone in rats in each group and changes in the number of bone cells. Calcein double labeling experiment showed the bone mineralization speed of mice in each group. Pure and stable mesoporous silica nanoparticles were successfully synthesized and miR-302b was successfully loaded into the nanoparticles. The osteoporotic fracture rat model was successfully created. In vivo experimental results showed that after injecting composite nanoparticles into mice, bone density and bone strength were significantly increased and osteoporotic fractures were improved. Mesoporous silica carries miR-302b to target DKK1 regulation, which can improve bone homeostasis imbalance in osteoporotic fractures. Composite nanoparticles can inhibit the expression of DKK1, promote bone formation, and inhibit bone resorption, thereby improving bone density and bone strength.
miR-302b 和 DKK1 是两种与骨代谢调节有关的分子。介孔二氧化硅是一种潜在的药物载体。本文旨在研究介孔二氧化硅携带miR-302b靶向DKK1调控改善骨质疏松性骨折患者骨平衡失调的机制。研究人员合成了介孔二氧化硅纳米颗粒并对其进行了表征，将miR-302b载入介孔二氧化硅形成复合纳米颗粒。进行了大鼠体内模型实验，以评估骨代谢。X射线检查和μCT扫描用于检测大鼠的骨含量和骨小梁状况。阿尔新蓝/红霉素/橙 G 染色用于观察胫骨干骺端骨小梁的变化。免疫组化染色显示了各组大鼠骨小梁的形成和骨细胞数量的变化。钙素双标记实验显示了各组小鼠的骨矿化速度。成功合成了纯净稳定的介孔二氧化硅纳米颗粒，并将 miR-302b 成功载入纳米颗粒。成功建立了骨质疏松性骨折大鼠模型。体内实验结果表明，小鼠注射复合纳米粒子后，骨密度和骨强度明显增加，骨质疏松性骨折得到改善。介孔二氧化硅携带miR-302b靶向调控DKK1，可改善骨质疏松性骨折的骨平衡失调。复合纳米粒子可抑制DKK1的表达，促进骨形成，抑制骨吸收，从而改善骨密度和骨强度。
{"title":"Improvement of Bone Homeostasis Imbalance in Osteoporotic Fractures by Mesoporous Silica Carrying miR-302b","authors":"Jiaqi Chu, Yuan Si, Song Shao","doi":"10.1166/jbn.2024.3808","DOIUrl":"https://doi.org/10.1166/jbn.2024.3808","url":null,"abstract":"miR-302b and DKK1 are two molecules related to the regulation of bone metabolism. Mesoporous silica is a potential drug carrier. This article aims to study the mechanism of mesoporous silica carrying miR-302b targeting DKK1 regulation to improve bone homeostasis imbalance in osteoporotic\u0000 fractures. Mesoporous silica nanoparticles were synthesized and characterized. miR-302b was loaded into mesoporous silica to form composite nanoparticles. In vivo rat model experiments were performed to evaluate bone metabolism. X-ray examination and μCT scan were used to\u0000 detect the bone content and trabecular bone status of rats. Alcian blue/hematoxylin/Orange G staining was used to observe changes in trabecular bone in the tibial metaphysis. Immunohistochemical staining showed the formation of trabecular bone in rats in each group and changes in the number\u0000 of bone cells. Calcein double labeling experiment showed the bone mineralization speed of mice in each group. Pure and stable mesoporous silica nanoparticles were successfully synthesized and miR-302b was successfully loaded into the nanoparticles. The osteoporotic fracture rat model was successfully\u0000 created. In vivo experimental results showed that after injecting composite nanoparticles into mice, bone density and bone strength were significantly increased and osteoporotic fractures were improved. Mesoporous silica carries miR-302b to target DKK1 regulation, which can improve\u0000 bone homeostasis imbalance in osteoporotic fractures. Composite nanoparticles can inhibit the expression of DKK1, promote bone formation, and inhibit bone resorption, thereby improving bone density and bone strength.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140353673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.
{"title":"Treatment Promotion of Osteoporotic Fractures by microRNA-320 Nanocapsules Through Stimulating Bone Marrow Mesenchymal Stem Cells","authors":"Ligang Qian, Qinggui Li, Qiao Ren","doi":"10.1166/jbn.2024.3784","DOIUrl":"https://doi.org/10.1166/jbn.2024.3784","url":null,"abstract":"We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal\u0000 stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment\u0000 of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic\u0000 defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140085360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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