Xun Che, Yu-Ling Wu, Qing-Ling He, Zhen Wu, Shi-Bo Wang, Han Zhang, M. Zubair Iqbal, Fei Liu, Jia-Ju Lu, Xiangdong Kong
The wound healing in the presence of bacterial infections is a complex process and prolonged challenge with severe implications for human health. To address this issue, injectable thermo-sensitive hydrogels as wound dressings have garnered considerable attention as a potential solution. In this study, we have developed an injectable and antibacterial thermosensitive hydrogel for wound healing, based on chitosan (CS) and β-glycerophosphate (β-GP) and modified with a range of cinnamaldehyde (CA). The CS/β-GP/CA gels demonstrate a phase transition at body temperatures, and exhibit good biocompatibility. Compared with the unmodified CS/β-GP hydrogels, incorporating CA can promote endothelial cell growth and the antibacterial property of the hydrogel system, which may lead to faster wound healing and tissue regeneration. Our findings suggest that the CS/β-GP/CA thermosensitive hydrogel emerges as a promising candidate for an injectable wound dressing in wound treatments.
{"title":"Synthesis of Injectable Thermosensitive Cinnamaldehyde-Loaded Chitosan Hydrogel for Antibacterial and Wound Dressing Application","authors":"Xun Che, Yu-Ling Wu, Qing-Ling He, Zhen Wu, Shi-Bo Wang, Han Zhang, M. Zubair Iqbal, Fei Liu, Jia-Ju Lu, Xiangdong Kong","doi":"10.1166/jbn.2024.3788","DOIUrl":"https://doi.org/10.1166/jbn.2024.3788","url":null,"abstract":"The wound healing in the presence of bacterial infections is a complex process and prolonged challenge with severe implications for human health. To address this issue, injectable thermo-sensitive hydrogels as wound dressings have garnered considerable attention as a potential solution.\u0000 In this study, we have developed an injectable and antibacterial thermosensitive hydrogel for wound healing, based on chitosan (CS) and β-glycerophosphate (β-GP) and modified with a range of cinnamaldehyde (CA). The CS/β-GP/CA gels demonstrate a phase transition\u0000 at body temperatures, and exhibit good biocompatibility. Compared with the unmodified CS/β-GP hydrogels, incorporating CA can promote endothelial cell growth and the antibacterial property of the hydrogel system, which may lead to faster wound healing and tissue regeneration. Our\u0000 findings suggest that the CS/β-GP/CA thermosensitive hydrogel emerges as a promising candidate for an injectable wound dressing in wound treatments.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhangsong Peng, Jie Chen, Yan Chen, Yuxin Chen, Yuheng Wang, Chao Han
Breast cancer stands as the foremost prevalent malignancy affecting women worldwide. The restoration of soft tissue volume is often necessary in cases of soft tissue loss, such as burn defects, surgical area defects, or posttumor resection defects, particularly in breast cancers. Conventional surgeries interventions, such as flap transplantation, can result in various complications and malformations, while prosthesis implantation may not be suitable due to skin weakness caused by radiation therapy. Autologous fat transplantation to the breast exhibits limited efficacy due to the unpredictable of resorption and low graft survival rates. Tissue engineering has emerged as a promising field offering novel therapeutic possibilities in regenerative medicine over the past two decades. Extensive exploration has been conducted on synthetic materials for adipose tissue engineering applications. This review delineates the current advancements and future directions in adipose tissue engineering strategies, cell applications, adipogenesismechanisms, and key factors, while focusing on the utilization of degradable synthetic polymers and the integration of 3D printing technologies.
{"title":"Progress of Degradable Synthetic Polymers in Adipose Tissue Engineering-A Review","authors":"Zhangsong Peng, Jie Chen, Yan Chen, Yuxin Chen, Yuheng Wang, Chao Han","doi":"10.1166/jbn.2024.3798","DOIUrl":"https://doi.org/10.1166/jbn.2024.3798","url":null,"abstract":"Breast cancer stands as the foremost prevalent malignancy affecting women worldwide. The restoration of soft tissue volume is often necessary in cases of soft tissue loss, such as burn defects, surgical area defects, or posttumor resection defects, particularly in breast cancers. Conventional\u0000 surgeries interventions, such as flap transplantation, can result in various complications and malformations, while prosthesis implantation may not be suitable due to skin weakness caused by radiation therapy. Autologous fat transplantation to the breast exhibits limited efficacy due to the\u0000 unpredictable of resorption and low graft survival rates. Tissue engineering has emerged as a promising field offering novel therapeutic possibilities in regenerative medicine over the past two decades. Extensive exploration has been conducted on synthetic materials for adipose tissue engineering\u0000 applications. This review delineates the current advancements and future directions in adipose tissue engineering strategies, cell applications, adipogenesismechanisms, and key factors, while focusing on the utilization of degradable synthetic polymers and the integration of 3D printing technologies.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140087533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Zhang, Jianfei Wang, Hao Dai, Ning Li, Fen Hu, Bolin Zhang, Yiming Yang, Haifeng Cai, Jinyin Yan
Cellulose nanopaper (CNP) is a kind of flat foldable film material composed of cellulose nanofibers. Cellulose nanopaper is based on nanocellulose (NC) as the basic unit, with excellent mechanical properties, thermal properties, optical properties and other characteristics, is a high-performance new material. This study discusses the mechanism of cis-platinum and CXCR4 siRNA carried with CNP in restraining the biological effect of breast carcinoma cells. CXCR4 siRNA carried with CNP was established and identified. The MCF-7 cells were divided into control set, si-CXCR4 set, cis-platinum set and united set randomly. The proliferative and apoptotic activity, CXCR4 presentation, presentation of EMT, and invasive ability were detected. mRNA and protein expression of CXCR4 was restrained by knockdown of CXCR4 notably. The proliferation was restrained and apoptosis was prompted. Expression of E-cadherin was increased and Zeb1 presentation was reduced. The invasive ability was restrained. The action was more notable in united set. Breast carcinoma cell proliferation was restrained and the apoptosis was prompted by cis-platinum and CXCR4 siRNA carried with CNP. The occurrence of EMT and invasion were therefore restrained. The sensibility of breast carcinoma on drugs was elevated.
{"title":"Cis-Platinum and CXCR4 siRNA Carried with Cellulose Nanopaper Restrain the Biological Effect of Breast Carcinoma Cells","authors":"Yang Zhang, Jianfei Wang, Hao Dai, Ning Li, Fen Hu, Bolin Zhang, Yiming Yang, Haifeng Cai, Jinyin Yan","doi":"10.1166/jbn.2024.3802","DOIUrl":"https://doi.org/10.1166/jbn.2024.3802","url":null,"abstract":"Cellulose nanopaper (CNP) is a kind of flat foldable film material composed of cellulose nanofibers. Cellulose nanopaper is based on nanocellulose (NC) as the basic unit, with excellent mechanical properties, thermal properties, optical properties and other characteristics, is a high-performance\u0000 new material. This study discusses the mechanism of cis-platinum and CXCR4 siRNA carried with CNP in restraining the biological effect of breast carcinoma cells. CXCR4 siRNA carried with CNP was established and identified. The MCF-7 cells were divided into control set, si-CXCR4 set, cis-platinum\u0000 set and united set randomly. The proliferative and apoptotic activity, CXCR4 presentation, presentation of EMT, and invasive ability were detected. mRNA and protein expression of CXCR4 was restrained by knockdown of CXCR4 notably. The proliferation was restrained and apoptosis was prompted.\u0000 Expression of E-cadherin was increased and Zeb1 presentation was reduced. The invasive ability was restrained. The action was more notable in united set. Breast carcinoma cell proliferation was restrained and the apoptosis was prompted by cis-platinum and CXCR4 siRNA carried with CNP. The\u0000 occurrence of EMT and invasion were therefore restrained. The sensibility of breast carcinoma on drugs was elevated.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanzheng Chen, Degan Lu, Wei Li, Xiaomin Dang, Xinju Li
In this study, we employed bioinformatics techniques to investigate the radiosensitization mechanisms of graphene oxide (GO) and silver-platinum (AgPt) nanocomposites (NCs) on microRNAs (miRNAs) in non-small cell lung cancer (NSCLC). The GO@AgPt nanocomposites were synthesized through a hydrothermal method involving graphene oxide. Characterization and structural analysis were performed using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Our experimental model was A549 cells, categorized into three groups: the blank group, control group, and GO@AgPt group. The blank group remained untreated, while the control group was exposed to 4Gy X-ray irradiation. The GO@AgPt group received 15 μg/mL GO@AgPt for 4 hours before exposure to 4Gy X-ray irradiation. Cellular RNA was extracted from each group, and a transcriptome sequencing library was constructed. Subsequent analysis identified differential genes, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway assessments. Our results revealed the uniform attachment of AgPt spherical nanoparticles (NPs) with an approximate diameter of 10 nm to the GO surface. The GO@AgPt nanocomposites comprised four single-layer GO sheets, each approximately 4 nm in thickness, with the Ag peak area being about six times that of the Pt peak area. A total of 197 miRNAs exhibited differential expression between the GO@AgPt and control groups, with 94 up-regulated and 103 down-regulatedmiRNAs. These miRNAs were associated with biological processes such as positive regulation of gene expression, cell surface interactions, and growth factor binding. Furthermore, they were implicated in various pathways, including microRNAs in cancer, fatty acid metabolism, human T-cell leukemia virus 1 infection, FoxO signaling, and alcoholic liver disease. Our findings demonstrate that GO@AgPt nanocomposites enhance the radio-sensitization effect in NSCLC, with 197 differentialmiRNAs participating in the process.
{"title":"Enhancing Radiosensitization of Non-Small Cell Lung Cancer with Graphene Oxide@AgPt Nanocomposites: A Bioinformatics Study","authors":"Nanzheng Chen, Degan Lu, Wei Li, Xiaomin Dang, Xinju Li","doi":"10.1166/jbn.2024.3797","DOIUrl":"https://doi.org/10.1166/jbn.2024.3797","url":null,"abstract":"In this study, we employed bioinformatics techniques to investigate the radiosensitization mechanisms of graphene oxide (GO) and silver-platinum (AgPt) nanocomposites (NCs) on microRNAs (miRNAs) in non-small cell lung cancer (NSCLC). The GO@AgPt nanocomposites were synthesized through\u0000 a hydrothermal method involving graphene oxide. Characterization and structural analysis were performed using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Our experimental\u0000 model was A549 cells, categorized into three groups: the blank group, control group, and GO@AgPt group. The blank group remained untreated, while the control group was exposed to 4Gy X-ray irradiation. The GO@AgPt group received 15 μg/mL GO@AgPt for 4 hours before exposure to 4Gy\u0000 X-ray irradiation. Cellular RNA was extracted from each group, and a transcriptome sequencing library was constructed. Subsequent analysis identified differential genes, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway assessments. Our results revealed the\u0000 uniform attachment of AgPt spherical nanoparticles (NPs) with an approximate diameter of 10 nm to the GO surface. The GO@AgPt nanocomposites comprised four single-layer GO sheets, each approximately 4 nm in thickness, with the Ag peak area being about six times that of the Pt peak area. A\u0000 total of 197 miRNAs exhibited differential expression between the GO@AgPt and control groups, with 94 up-regulated and 103 down-regulatedmiRNAs. These miRNAs were associated with biological processes such as positive regulation of gene expression, cell surface interactions, and growth factor\u0000 binding. Furthermore, they were implicated in various pathways, including microRNAs in cancer, fatty acid metabolism, human T-cell leukemia virus 1 infection, FoxO signaling, and alcoholic liver disease. Our findings demonstrate that GO@AgPt nanocomposites enhance the radio-sensitization effect\u0000 in NSCLC, with 197 differentialmiRNAs participating in the process.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lei Yang, Jiaxin Liu, Wene Zhao, Amaia Huguet-Casquero, Ander Pino Castresana, J. Pedraz, Murugan Ramalingam, Shuwei Li
Objective: To explore whether shikonin can alleviate vascular leakage in burn wounds, inhibit skin inflammation, and exert protective effects on skin. Methods: A mouse skin burn wound model was routinely established, and fluorescent microspheres were injected through the tail vein 2 h before sampling to characterize the degree of vascular leakage. Dorsal skin was obtained by surgical dissection and embedded in OCT, and frozen sections were prepared. CD31 immunofluorescence was used to determine the distribution of blood vessels in burnt skin, and Ly6G immunofluorescence staining was used to determine the level of neutrophil recruitment in the skin. Results: Skin microvessels were mainly distributed in the dermis. We found that severe vascular leakage occurred in the blood vessels of the burned dermis, and shikonin significantly alleviated vascular leakage in the burned area. Furthermore, shikonin significantly inhibited neutrophil recruitment to burn sites. Most importantly, we also found that shikonin can alleviate vascular leakage and inhibit skin inflammation at burn sites through the Wnt/β-catenin signaling pathway. Conclusion: Shikonin can alleviate vascular leakage, and inhibit skin inflammation in burn wounds through the Wnt/β-catenin signaling pathway. This experimental study provides a proof-of-concept and a new avenue for the repair and treatment of burn skin vascular injuries.
{"title":"Shikonin Inhibits Vascular Extravasation and Inflammation in Burn Wounds by Regulating Wnt/β-Catenin Pathway","authors":"Lei Yang, Jiaxin Liu, Wene Zhao, Amaia Huguet-Casquero, Ander Pino Castresana, J. Pedraz, Murugan Ramalingam, Shuwei Li","doi":"10.1166/jbn.2024.3789","DOIUrl":"https://doi.org/10.1166/jbn.2024.3789","url":null,"abstract":"Objective: To explore whether shikonin can alleviate vascular leakage in burn wounds, inhibit skin inflammation, and exert protective effects on skin. Methods: A mouse skin burn wound model was routinely established, and fluorescent microspheres were injected through the\u0000 tail vein 2 h before sampling to characterize the degree of vascular leakage. Dorsal skin was obtained by surgical dissection and embedded in OCT, and frozen sections were prepared. CD31 immunofluorescence was used to determine the distribution of blood vessels in burnt skin, and Ly6G immunofluorescence\u0000 staining was used to determine the level of neutrophil recruitment in the skin. Results: Skin microvessels were mainly distributed in the dermis. We found that severe vascular leakage occurred in the blood vessels of the burned dermis, and shikonin significantly alleviated vascular\u0000 leakage in the burned area. Furthermore, shikonin significantly inhibited neutrophil recruitment to burn sites. Most importantly, we also found that shikonin can alleviate vascular leakage and inhibit skin inflammation at burn sites through the Wnt/β-catenin signaling pathway.\u0000 Conclusion: Shikonin can alleviate vascular leakage, and inhibit skin inflammation in burn wounds through the Wnt/β-catenin signaling pathway. This experimental study provides a proof-of-concept and a new avenue for the repair and treatment of burn skin vascular injuries.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140090640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhaodan Ye, Mengchen Song, Adel Al-Kalei, Wenjing Wang, G. Cao
In this study, we evaluate the effectiveness and safety of conventional transcatheter arterial chemoembolization (C-TACE) and drug-loaded microsphere-based TACE (DEB-TACE) in patients with hepatocellular carcinoma (HCC) accompanied by portal vein tumor thrombus (PVTT). A total of 51 HCC patients with PVTT treated at our hospital between August 2016 and December 2021 were collected and retrospectively analyzed. 39 patients were in the DEB-TACE group and 9 patients were in the C-TACE group, after excluding 3 patients. Serum alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) levels were assessed using magnetic nanoparticle-based chemiluminescence immunoassay. The liver function was evaluated before TACE, at 1 month, and at 3 months after treatment. The therapeutic response and intraoperative and postoperative adverse events were analyzed in two groups. Both TACE treatments effectively reduced tumor thrombus size, tumor count, and PVTT classification, with DEB-TACE demonstrating superior efficacy. The median survival time was slightly longer in the DEB-TACE group (14 months) compared to the c-TACE group (11 months), but there were no significant differences in the survival curves. Both groups experienced decreased postoperative AFP levels, while PIVKA-II levels remained stable without significant differences between the two groups. The PIVKA-II index exhibited no significant alteration, and there was no notable disparity observed between the two groups. There were no statistically significant differences observed in liver function and postoperative adverse reactions between the two groups after the operation. In conclusion, the therapeutic effect and safety of DEB-TACE are found to be equivalent to those of C-TACE.
{"title":"Efficacy and Safety of Drug-Loaded Microsphere-Based Transcatheter Arterial Chemoembolization for the Treatment of Hepatocellular Carcinoma with Portal Vein Tumor Thrombus","authors":"Zhaodan Ye, Mengchen Song, Adel Al-Kalei, Wenjing Wang, G. Cao","doi":"10.1166/jbn.2024.3778","DOIUrl":"https://doi.org/10.1166/jbn.2024.3778","url":null,"abstract":"In this study, we evaluate the effectiveness and safety of conventional transcatheter arterial chemoembolization (C-TACE) and drug-loaded microsphere-based TACE (DEB-TACE) in patients with hepatocellular carcinoma (HCC) accompanied by portal vein tumor thrombus (PVTT). A total of 51\u0000 HCC patients with PVTT treated at our hospital between August 2016 and December 2021 were collected and retrospectively analyzed. 39 patients were in the DEB-TACE group and 9 patients were in the C-TACE group, after excluding 3 patients. Serum alpha-fetoprotein (AFP) and protein induced by\u0000 vitamin K absence or antagonist-II (PIVKA-II) levels were assessed using magnetic nanoparticle-based chemiluminescence immunoassay. The liver function was evaluated before TACE, at 1 month, and at 3 months after treatment. The therapeutic response and intraoperative and postoperative adverse\u0000 events were analyzed in two groups. Both TACE treatments effectively reduced tumor thrombus size, tumor count, and PVTT classification, with DEB-TACE demonstrating superior efficacy. The median survival time was slightly longer in the DEB-TACE group (14 months) compared to the c-TACE group\u0000 (11 months), but there were no significant differences in the survival curves. Both groups experienced decreased postoperative AFP levels, while PIVKA-II levels remained stable without significant differences between the two groups. The PIVKA-II index exhibited no significant alteration, and\u0000 there was no notable disparity observed between the two groups. There were no statistically significant differences observed in liver function and postoperative adverse reactions between the two groups after the operation. In conclusion, the therapeutic effect and safety of DEB-TACE are found\u0000 to be equivalent to those of C-TACE.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current study, Laurus nobilis extract (ethanolic) was loaded into chitosan nanoparticles and studied for their potential anticancer activity against GC1436 cells using SEM images of LNECHNPs and CHNPs. In vitro studies showed that gastric cancer cells cultured with the nanoparticle system suppressed the proliferation and migration activity of cancer cells through suppressing the PI3K/Akt/mTOR signaling pathway. The SEM images display that both LNECHNPs and CHNPs are round and possess a smooth surface. The smooth surface is a probable outcome of the manufacturing process used to create the nanoparticles, which can employ procedures like sol–gel or chemical reduction methods. Gene expression studies showed that the expression levels of PI3K and Akt genes were significantly reduced in GC1436 cells cultured with LNECHNPs Nephrotoxicity of the nanoparticles was studied in a rat model. In vivo study showed that the intravenous administration of the nanoparticle system did not impart significant nephrotoxicity in the animals 7 days post-injection. These results provide important insights into the safety of these nanocarrier systems, which could be beneficial in their potential application in biomedical and pharmaceutical fields.
{"title":"In vitro and In vivo Characterization of Laurus nobilis Loaded Chitosan Nanoparticles as a Potential Treatment for Gastric Cancer Using SEM Images, PCR and Histopathology","authors":"Qunguang Gao, Xuegang Liu, Zhiguang Wang","doi":"10.1166/jbn.2024.3743","DOIUrl":"https://doi.org/10.1166/jbn.2024.3743","url":null,"abstract":"In the current study, Laurus nobilis extract (ethanolic) was loaded into chitosan nanoparticles and studied for their potential anticancer activity against GC1436 cells using SEM images of LNECHNPs and CHNPs. In vitro studies showed that gastric cancer cells cultured with\u0000 the nanoparticle system suppressed the proliferation and migration activity of cancer cells through suppressing the PI3K/Akt/mTOR signaling pathway. The SEM images display that both LNECHNPs and CHNPs are round and possess a smooth surface. The smooth surface is a probable outcome of the manufacturing\u0000 process used to create the nanoparticles, which can employ procedures like sol–gel or chemical reduction methods. Gene expression studies showed that the expression levels of PI3K and Akt genes were significantly reduced in GC1436 cells cultured with LNECHNPs Nephrotoxicity of the nanoparticles\u0000 was studied in a rat model. In vivo study showed that the intravenous administration of the nanoparticle system did not impart significant nephrotoxicity in the animals 7 days post-injection. These results provide important insights into the safety of these nanocarrier systems, which\u0000 could be beneficial in their potential application in biomedical and pharmaceutical fields.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: ultrasound imaging based on nanobubbles was used as the examination method in this study. Abdominal examination was performed on patients with and without diabetes during pregnancy, and the examination data between patients in different groups were compared and analyzed. Methods: 74 pregnant women were selected and enrolled in this study. Thirty-five pregnant women with diabetes were enrolled in the experimental group (Exp group) and 39 pregnant women without diabetes were assigned to the control group (Ctrl group). Results: there were no significant differences in fetal abdominal circumference, femoral length, and biparietal diameter between the two groups. The liver length and head circumference in the Exp group were higher based on the Ctrl group, with a statistical significance. Moreover, the occurrence probability of abnormal fetal growth and development indexes in the Exp group was higher and exhibited a statistical significance to that in the Ctrl group. Conclusion: patients with diabetes were more likely to have abnormal pregnancies. It is necessary to adopt color examination for clinical screening of diabetic pregnant women, so that the life safety of pregnant women and fetus can be guaranteed, reflecting the good application prospect of ultrasound examination.
{"title":"Comparative Analysis of Abnormal Pregnancy Between Non-Diabetic Pregnant Women and Diabetic Pregnant Women by Using Nanobubbles-Based Ultrasound Images","authors":"Jue Wu, Jun Liu, Hongping Ou, Zheyu Hao","doi":"10.1166/jbn.2024.3510","DOIUrl":"https://doi.org/10.1166/jbn.2024.3510","url":null,"abstract":"Objective: ultrasound imaging based on nanobubbles was used as the examination method in this study. Abdominal examination was performed on patients with and without diabetes during pregnancy, and the examination data between patients in different groups were compared and analyzed.\u0000 Methods: 74 pregnant women were selected and enrolled in this study. Thirty-five pregnant women with diabetes were enrolled in the experimental group (Exp group) and 39 pregnant women without diabetes were assigned to the control group (Ctrl group). Results: there were no significant\u0000 differences in fetal abdominal circumference, femoral length, and biparietal diameter between the two groups. The liver length and head circumference in the Exp group were higher based on the Ctrl group, with a statistical significance. Moreover, the occurrence probability of abnormal fetal\u0000 growth and development indexes in the Exp group was higher and exhibited a statistical significance to that in the Ctrl group. Conclusion: patients with diabetes were more likely to have abnormal pregnancies. It is necessary to adopt color examination for clinical screening of diabetic\u0000 pregnant women, so that the life safety of pregnant women and fetus can be guaranteed, reflecting the good application prospect of ultrasound examination.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140085139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fan Mei, Dan Qian, Yujun Nie, Bin Wang, Aimin Liang, Hua Li
We aimed to investigate the biomedical methods of wavelet transform algorithm on ultrasonic image denoising algorithm and the risk factors for the adverse prognostic events of patients with myocarditis and analyse its correlation with free triiodothyronine (FT3) level. A retrospective study was performed to include 68 patients diagnosed with acute myocarditis (AM). The included patients were enrolled into adverse event (AE) group (n = 7) and non-adverse event (NAE) group (n = 61). The clinical data, laboratory examination indicators, echocardiographic parameters, and thyroid functions between the patients in the two groups at admission were compared. Besides, wavelet transform (WT) algorithm was employed to process ultrasonic images containing noises. Univariate and multivariate analysis were performed using Logistic regression model. It was demonstrated that peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) (35.279, 0.847) of wavelet transform algorithm were higher than those of denoising convolutional neural network (DnCNN) algorithm (30.673, 0.582) and Red-Net algorithm (28.489, 0.638). In the AE group, the QRS interval was longer ([102 (93, 135) ms] versus [86 (79, 102) ms]), the proportion of prolonged QRS period was higher (28.57%, 9.83%), and the creatine kinase isoenzyme, a marker of myocardial injury, was higher [32.87 (13.64, 78.62) U/L] versus 12.02 (9.89, 27.65) U/L], and the level of FT3 was lower [1.87 (1.23, 2.36) pg/mL versus 2.83 (1.83, 3.45) pg/mL] compared to the NAE group. The left ventricular ejection fraction (LVEF) in the adverse event group was lower than that in the non-adverse event group [45.78 (36.18, 54.32) % versus 63.72 (54.82, 64.68)]. Multivariate Logistic regression analysis showed that the risk factors for adverse events in patients with acute myocarditis included QRS interval > 120 ms (OR = 1.021), creatine kinase isoenzyme > 24 U/L (OR = 1.024), FT3 (OR = 0.067), and LVEF < 50% (OR = 0.973). This work confirmed that the wavelet transform algorithm can optimize the image quality of echocardiography, improve the clarity, and provide a feasible idea for improving the prognosis of patients with acute myocarditis.
{"title":"Biomedical Applications of Wavelet Transform Algorithm on Deep Learning Ultrasonic Image Optimization as a Prognosis Model for Acute Myocarditis","authors":"Fan Mei, Dan Qian, Yujun Nie, Bin Wang, Aimin Liang, Hua Li","doi":"10.1166/jbn.2024.3787","DOIUrl":"https://doi.org/10.1166/jbn.2024.3787","url":null,"abstract":"We aimed to investigate the biomedical methods of wavelet transform algorithm on ultrasonic image denoising algorithm and the risk factors for the adverse prognostic events of patients with myocarditis and analyse its correlation with free triiodothyronine (FT3) level. A retrospective\u0000 study was performed to include 68 patients diagnosed with acute myocarditis (AM). The included patients were enrolled into adverse event (AE) group (n = 7) and non-adverse event (NAE) group (n = 61). The clinical data, laboratory examination indicators, echocardiographic parameters,\u0000 and thyroid functions between the patients in the two groups at admission were compared. Besides, wavelet transform (WT) algorithm was employed to process ultrasonic images containing noises. Univariate and multivariate analysis were performed using Logistic regression model. It was demonstrated\u0000 that peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) (35.279, 0.847) of wavelet transform algorithm were higher than those of denoising convolutional neural network (DnCNN) algorithm (30.673, 0.582) and Red-Net algorithm (28.489, 0.638). In the AE group, the QRS interval\u0000 was longer ([102 (93, 135) ms] versus [86 (79, 102) ms]), the proportion of prolonged QRS period was higher (28.57%, 9.83%), and the creatine kinase isoenzyme, a marker of myocardial injury, was higher [32.87 (13.64, 78.62) U/L] versus 12.02 (9.89, 27.65) U/L], and the level of FT3 was lower\u0000 [1.87 (1.23, 2.36) pg/mL versus 2.83 (1.83, 3.45) pg/mL] compared to the NAE group. The left ventricular ejection fraction (LVEF) in the adverse event group was lower than that in the non-adverse event group [45.78 (36.18, 54.32) % versus 63.72 (54.82, 64.68)]. Multivariate Logistic regression\u0000 analysis showed that the risk factors for adverse events in patients with acute myocarditis included QRS interval > 120 ms (OR = 1.021), creatine kinase isoenzyme > 24 U/L (OR = 1.024), FT3 (OR = 0.067), and LVEF < 50% (OR = 0.973). This work confirmed that the wavelet transform algorithm\u0000 can optimize the image quality of echocardiography, improve the clarity, and provide a feasible idea for improving the prognosis of patients with acute myocarditis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaodong Wang, Yanli Yang, Xiaowen Meng, Fuhai Ji, Cunxian Shi
Liposomes present a promising strategy for microRNA (miRNA) delivery, capitalizing on their unique properties to enable effective therapeutic interventions. In this study, we investigate lipid nanoparticles (LNPs) as carriers to delivery miR-22, aiming to mitigate neuronal pyroptosis by targeting nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3). In vitro, HT-22 cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to assess cell viability, lactate dehydrogenase (LDH) levels, and pyroptosis. The pyroptosis-related protein expression was determined by Western blot analysis. The interaction between miR-22 and NLRP3 was assessed by dual luciferase assays. LNPs were employed to deliver miR-22 precursor oligonucleotides (LNP/miR-22) to HT-22 cells. miR-22 overexpression models were constructed to investigate its impact on OGD/R-induced pyroptosis. In vivo, a mouse model of cerebral ischemia-reperfusion was established to investigate the effects of LNP/miR-22 treatment, NLRP3 inhibitor (MCC950), or NLRP3 activator (Nigericin sodium salt). Neural damage and pyroptosis in the hippocampi were evaluated using staining techniques and immunofluorescence. The expression levels of pyroptosis-related proteins in the hippocampi were analyzed by western blotting. Results demonstrated that OGD/R reduced cell viability, increased LDH levels, and induced pyroptosis In vitro. NLRP3 overexpression exacerbated OGD/R-induced pyroptosis. miR-22 was found to target and downregulate NLRP3 expression, leading to reduced pyroptosis. In vivo, miR-22 overexpression suppressed NLRP3 activation, effectively attenuating pyroptosis. In conclusion, LNP-mediated delivery of miR-22 offers a promising strategy to alleviate neuronal pyroptosis by targeting NLRP3, holding potential for the treatment of cerebral ischemia-reperfusion injury.
{"title":"Regulation of Neuronal Pyroptosis Through NLRP3 by Delivering miR-22 Using Lipid Nanoparticles in Mice with Cerebral Ischemia-Reperfusion Injury","authors":"Xiaodong Wang, Yanli Yang, Xiaowen Meng, Fuhai Ji, Cunxian Shi","doi":"10.1166/jbn.2024.3786","DOIUrl":"https://doi.org/10.1166/jbn.2024.3786","url":null,"abstract":"Liposomes present a promising strategy for microRNA (miRNA) delivery, capitalizing on their unique properties to enable effective therapeutic interventions. In this study, we investigate lipid nanoparticles (LNPs) as carriers to delivery miR-22, aiming to mitigate neuronal pyroptosis\u0000 by targeting nucleotide-binding oligomerization domain (NOD)-like receptor family pyrin domain containing 3 (NLRP3). In vitro, HT-22 cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to assess cell viability, lactate dehydrogenase (LDH) levels, and pyroptosis.\u0000 The pyroptosis-related protein expression was determined by Western blot analysis. The interaction between miR-22 and NLRP3 was assessed by dual luciferase assays. LNPs were employed to deliver miR-22 precursor oligonucleotides (LNP/miR-22) to HT-22 cells. miR-22 overexpression models were\u0000 constructed to investigate its impact on OGD/R-induced pyroptosis. In vivo, a mouse model of cerebral ischemia-reperfusion was established to investigate the effects of LNP/miR-22 treatment, NLRP3 inhibitor (MCC950), or NLRP3 activator (Nigericin sodium salt). Neural damage and pyroptosis\u0000 in the hippocampi were evaluated using staining techniques and immunofluorescence. The expression levels of pyroptosis-related proteins in the hippocampi were analyzed by western blotting. Results demonstrated that OGD/R reduced cell viability, increased LDH levels, and induced pyroptosis\u0000 In vitro. NLRP3 overexpression exacerbated OGD/R-induced pyroptosis. miR-22 was found to target and downregulate NLRP3 expression, leading to reduced pyroptosis. In vivo, miR-22 overexpression suppressed NLRP3 activation, effectively attenuating pyroptosis. In conclusion, LNP-mediated\u0000 delivery of miR-22 offers a promising strategy to alleviate neuronal pyroptosis by targeting NLRP3, holding potential for the treatment of cerebral ischemia-reperfusion injury.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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