Qianhao Zhu, Rikao Yu, Renqiang He, Dawei Song, Weihua Liu
We investigated the role of the lncRNA MSC-AS1 in prostate cancer (PCa) by analyzing its expression in 56 pairs of PCa tissues and adjacent tissues. We examined the relationship between MSC-AS1 expression, clinicopathological indicators, and patient prognosis. In PCa cell lines, we overexpressed or knocked down MSC-AS1 and assessed its impact on cell function using transwell assays and wound healing tests. We explored the interaction between MSC-AS1 and miR-190a-3p using luciferase reporter assays. Our findings showed significantly higher MSC-AS1 expression in PCa tumor specimens compared to adjacent tissues. High MSC-AS1 expression correlated with increased incidence of lymph node and distant metastasis. Overexpressing MSC-AS1 reduced cell invasiveness and migration, while knocking it down enhanced these abilities. We observed decreased miR-190a-3p expression in PCa tissues, negatively correlating with MSC-AS1 expression. Modulating miR-190a-3p expression counteracted the effects of MSC-AS1 on cell invasiveness and migration. In conclusion, our study highlights the association of MSC-AS1 with metastasis and poor prognosis in PCa patients, suggesting its involvement in the malignant progression of the disease via miR-190a-3p modulation. MSC-AS1 holds potential as a prognostic biomarker for PCa and a therapeutic target for novel treatment strategies. Further research is needed to understand the underlying mechanisms and validate the clinical implications of targeting MSC-AS1 and miR-190a-3p in PCa management.
{"title":"Long Chain Non-Coding RNA MSC-AS1 Promote Invasion and Migration of Prostate Cancer Through Regulating microRNA-190a-3p","authors":"Qianhao Zhu, Rikao Yu, Renqiang He, Dawei Song, Weihua Liu","doi":"10.1166/jbn.2023.3683","DOIUrl":"https://doi.org/10.1166/jbn.2023.3683","url":null,"abstract":"We investigated the role of the lncRNA MSC-AS1 in prostate cancer (PCa) by analyzing its expression in 56 pairs of PCa tissues and adjacent tissues. We examined the relationship between MSC-AS1 expression, clinicopathological indicators, and patient prognosis. In PCa cell lines, we overexpressed or knocked down MSC-AS1 and assessed its impact on cell function using transwell assays and wound healing tests. We explored the interaction between MSC-AS1 and miR-190a-3p using luciferase reporter assays. Our findings showed significantly higher MSC-AS1 expression in PCa tumor specimens compared to adjacent tissues. High MSC-AS1 expression correlated with increased incidence of lymph node and distant metastasis. Overexpressing MSC-AS1 reduced cell invasiveness and migration, while knocking it down enhanced these abilities. We observed decreased miR-190a-3p expression in PCa tissues, negatively correlating with MSC-AS1 expression. Modulating miR-190a-3p expression counteracted the effects of MSC-AS1 on cell invasiveness and migration. In conclusion, our study highlights the association of MSC-AS1 with metastasis and poor prognosis in PCa patients, suggesting its involvement in the malignant progression of the disease via miR-190a-3p modulation. MSC-AS1 holds potential as a prognostic biomarker for PCa and a therapeutic target for novel treatment strategies. Further research is needed to understand the underlying mechanisms and validate the clinical implications of targeting MSC-AS1 and miR-190a-3p in PCa management.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"111 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To improve the detection rate of non-small-cell lung cancer (NSCLC) exfoliated cells in pleural effusion, we designed nano-MSN-DNA fluorescent probes that could efficiently bind to mutated oncogenes in tumor cells. Mutated NSCLC cells could be detected directly by fluorescence intensity through confocal microscopy without using conventional polymerase chain reaction (PCR). In addition, the DNA probe was highly permeable in NSCLC cells and was stable in methanol at low temperatures. Using the nano-MSN-DNA fluorescent probes, we detected a significantly higher incidence of epidermal growth factor receptor (EGFR) and KRAS mutations in NSCLC pleural effusions and cells compared to those in normal patients, especially in lung adenocarcinoma cells. EGFR and KRAS mutations were more likely to occur in poorly differentiated and clinically advanced NSCLC, and the mutations enhanced tumor aggressiveness, leading to poor prognosis. The nano-MSN-DNA fluorescent probe was significantly more sensitive than Wright staining for screening pleural fluid exfoliated lung squamous carcinoma and adenocarcinoma cells. Thus, the nano-MSN-DNA fluorescent probe shows great potential for screening exfoliated cells from pleural fluid of patients with lung cancer and guiding targeted therapies.
{"title":"Analysis of <i>In Situ</i> Fluorescent Probes for Rapid Screening of Epidermal Growth Factor Receptor and KRAS Mutations in Exfoliated Cells from Pleural Fluid in Patients with Lung Cancer","authors":"Yaozheng Zhou, Qing Wang, Linxia Xie, Sha Lu","doi":"10.1166/jbn.2023.3687","DOIUrl":"https://doi.org/10.1166/jbn.2023.3687","url":null,"abstract":"To improve the detection rate of non-small-cell lung cancer (NSCLC) exfoliated cells in pleural effusion, we designed nano-MSN-DNA fluorescent probes that could efficiently bind to mutated oncogenes in tumor cells. Mutated NSCLC cells could be detected directly by fluorescence intensity through confocal microscopy without using conventional polymerase chain reaction (PCR). In addition, the DNA probe was highly permeable in NSCLC cells and was stable in methanol at low temperatures. Using the nano-MSN-DNA fluorescent probes, we detected a significantly higher incidence of epidermal growth factor receptor (EGFR) and KRAS mutations in NSCLC pleural effusions and cells compared to those in normal patients, especially in lung adenocarcinoma cells. EGFR and KRAS mutations were more likely to occur in poorly differentiated and clinically advanced NSCLC, and the mutations enhanced tumor aggressiveness, leading to poor prognosis. The nano-MSN-DNA fluorescent probe was significantly more sensitive than Wright staining for screening pleural fluid exfoliated lung squamous carcinoma and adenocarcinoma cells. Thus, the nano-MSN-DNA fluorescent probe shows great potential for screening exfoliated cells from pleural fluid of patients with lung cancer and guiding targeted therapies.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"158 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135707291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wenji Li, Shu Huang, Lihong Xie, Yilin Zhai, Fengqiu Gong
This study was established to explore the therapeutic effect of nanoknife ablation in the treatment of pancreatic cancer with comprehensive perioperative nursing. The clinical data of 92 patients with pancreatic cancer who underwent nanoknife ablation surgery in The First Affiliated Hospital, Sun Yat-sen University and Sun Yat-sen University Cancer Center from January 2015 to December 2018 were retrospectively studied. These 92 patients were randomly and equally divided into a group with comprehensive perioperative nursing care and a group with routine nursing care. After the intervention period, the satisfaction with the nursing, serum albumin, total protein, and hemoglobin levels, length of hospital stay, and incidence of postoperative complications were compared between the two groups. Meanwhile, blood biochemical indicators and related immune indicators of 30 patients in the comprehensive nursing group were analyzed and compared between before and after the operation. Results: The hospitalization time of patients in the routine care group was significantly longer than that in the comprehensive care group ( P < 0.05). Meanwhile, the levels of albumin, total protein, and hemoglobin in the serum of patients in the comprehensive care group were significantly higher than those in the routine care group ( P < 0.05). All patients exhibited a certain increase in serum amylase level on the day after the operation, which then gradually decreased. There was no statistically significant difference in myocardial enzyme indexes at different time points before and after the operation. The glycoprotein antigen tumor markers carbohydrate antigen (CA)-19-9 and CA-242 increased on the 1st day after surgery, and gradually decreased thereafter. This study indicated that the safety of nanoknife ablation in patients with pancreatic cancer is relatively high. In addition, there is a clear clinical effect of comprehensive nursing care in the perioperative period, which can effectively improve the levels of albumin, total protein, and hemoglobin, shorten the hospitalization time, and reduce the incidence of postoperative complications. Such nursing care also relieved the tumor-related immunosuppression after surgery and significantly decreased the levels of tumor markers.
{"title":"Effect of Nanoknife Ablation Therapy in Patients with Pancreatic Cancer","authors":"Wenji Li, Shu Huang, Lihong Xie, Yilin Zhai, Fengqiu Gong","doi":"10.1166/jbn.2023.3675","DOIUrl":"https://doi.org/10.1166/jbn.2023.3675","url":null,"abstract":"This study was established to explore the therapeutic effect of nanoknife ablation in the treatment of pancreatic cancer with comprehensive perioperative nursing. The clinical data of 92 patients with pancreatic cancer who underwent nanoknife ablation surgery in The First Affiliated Hospital, Sun Yat-sen University and Sun Yat-sen University Cancer Center from January 2015 to December 2018 were retrospectively studied. These 92 patients were randomly and equally divided into a group with comprehensive perioperative nursing care and a group with routine nursing care. After the intervention period, the satisfaction with the nursing, serum albumin, total protein, and hemoglobin levels, length of hospital stay, and incidence of postoperative complications were compared between the two groups. Meanwhile, blood biochemical indicators and related immune indicators of 30 patients in the comprehensive nursing group were analyzed and compared between before and after the operation. Results: The hospitalization time of patients in the routine care group was significantly longer than that in the comprehensive care group ( P < 0.05). Meanwhile, the levels of albumin, total protein, and hemoglobin in the serum of patients in the comprehensive care group were significantly higher than those in the routine care group ( P < 0.05). All patients exhibited a certain increase in serum amylase level on the day after the operation, which then gradually decreased. There was no statistically significant difference in myocardial enzyme indexes at different time points before and after the operation. The glycoprotein antigen tumor markers carbohydrate antigen (CA)-19-9 and CA-242 increased on the 1st day after surgery, and gradually decreased thereafter. This study indicated that the safety of nanoknife ablation in patients with pancreatic cancer is relatively high. In addition, there is a clear clinical effect of comprehensive nursing care in the perioperative period, which can effectively improve the levels of albumin, total protein, and hemoglobin, shorten the hospitalization time, and reduce the incidence of postoperative complications. Such nursing care also relieved the tumor-related immunosuppression after surgery and significantly decreased the levels of tumor markers.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdominal aortic aneurysm (AAA) is a pathological condition of chronic dilation of the aorta. This study investigated the mechanism underlying the inhibitory effect of resveratrol nanoparticles on AAA endothelial dysfunction. Mice in the control group received normal saline ( n = 18) while those in the model group ( n = 18) were implanted with Alzet micropumps to induce AAA. After modeling, the endothelial cells of abdominal aortic tissue were collected and treated with resveratrol nanoparticles and resveratrol nanoparticles plus sirtuin 1 (SIRT1) (resveratrol nanoparticles+SIRT1 group). CCK-8 method detected proliferation ability of abdominal aortic endothelial cells, flow cytometry assessed cell apoptosis, and transwell method measured the migration ability along with analysis of SIRT1 level, eNOS and NO content. The proliferation ability of endothelial cells was significantly decreased in resveratrol nanoparticles group (0.41±0.04, 0.60±0.05, 0.69±0.04) and resveratrol+SIRT1 group (0.37±0.05, 0.49±0.04, 0.57±0.04), with lower proliferation in resveratrol+SIRT1 group ( P <0.05). Treatment resulted in enhancement of endothelial cell apoptosis and decreased migration ability ( P <0.05), as the effect of combined treatment was more significant. Moreover, resveratrol nanoparticles (0.44±0.02, 0.34±0.05) or resveratrol nanoparticles plus SIRT1 (0.50±0.01, 0.44±0.03) increased SIRT1 level ( P <0.05), eNOS activity and NO secretion ( P <0.05) in the resveratrol+SIRT1 group. Resveratrol nanoparticles can reduce endothelial cell proliferation and migration and induce apoptosis when increasing SIRT1 expression.
{"title":"Resveratrol Nanoparticles Inhibit Endothelial Dysfunction in Abdominal Aortic Aneurysm by Preventing eNOS Uncoupling Through Sirtuin 1 Pathway","authors":"Zhenhuan Chen, Qinzhi Xiong, Junke Luo, Zhiyong Wu, Junfeng Zhan","doi":"10.1166/jbn.2023.3681","DOIUrl":"https://doi.org/10.1166/jbn.2023.3681","url":null,"abstract":"Abdominal aortic aneurysm (AAA) is a pathological condition of chronic dilation of the aorta. This study investigated the mechanism underlying the inhibitory effect of resveratrol nanoparticles on AAA endothelial dysfunction. Mice in the control group received normal saline ( n = 18) while those in the model group ( n = 18) were implanted with Alzet micropumps to induce AAA. After modeling, the endothelial cells of abdominal aortic tissue were collected and treated with resveratrol nanoparticles and resveratrol nanoparticles plus sirtuin 1 (SIRT1) (resveratrol nanoparticles+SIRT1 group). CCK-8 method detected proliferation ability of abdominal aortic endothelial cells, flow cytometry assessed cell apoptosis, and transwell method measured the migration ability along with analysis of SIRT1 level, eNOS and NO content. The proliferation ability of endothelial cells was significantly decreased in resveratrol nanoparticles group (0.41±0.04, 0.60±0.05, 0.69±0.04) and resveratrol+SIRT1 group (0.37±0.05, 0.49±0.04, 0.57±0.04), with lower proliferation in resveratrol+SIRT1 group ( P <0.05). Treatment resulted in enhancement of endothelial cell apoptosis and decreased migration ability ( P <0.05), as the effect of combined treatment was more significant. Moreover, resveratrol nanoparticles (0.44±0.02, 0.34±0.05) or resveratrol nanoparticles plus SIRT1 (0.50±0.01, 0.44±0.03) increased SIRT1 level ( P <0.05), eNOS activity and NO secretion ( P <0.05) in the resveratrol+SIRT1 group. Resveratrol nanoparticles can reduce endothelial cell proliferation and migration and induce apoptosis when increasing SIRT1 expression.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laryngopharyngeal reflux (LPR) and gastroesophageal reflux disease (GERD) involve in head and neck diseases. Their association with carcinogenesis of head and neck squamous cell carcinoma (HNSCC) has attracted increasing attention in recent years. We established a risk prediction model based on LPR/GERD-related genes (RRGs), to investigate the relationship between LPR/GERD and HNSCC, and explore their relevant mechanisms. To establish the risk model, the TCGA-HNSC project from the Cancer Genome Atlas (TCGA) database was used to conduct training and internal validation assays, and the GSE65858 project and five other TCGA projects involving different kinds of tumors were used to conduct the external validation assays. Twelve RRGs were determined to construct risk signature. Kaplan–Meier curves and receiver operating characteristic curves for the model were used to predict 1-, 3-, and 5-year overall survival (OS), particularly for patients with laryngeal squamous cell carcinoma (LSCC). The proportions of immune cells in high-risk group, including T cells, CD8+ T cells, NK cells, myeloid dendritic cells, B lineage, and monocytic lineage, were significantly lower ( P <0.05). Interestingly, risk score was negatively associated with immune checkpoint-related genes expression. The predicted therapeutic sensitivity of immune checkpoint inhibitors (ICIs) in high-risk group was lower (7.27±1.08 vs. 7.80±1.12, P = 4.6×10 −6 ). Moreover, the predicted IC50 of Erlotinib (EGFR inhibitor) and Parthenolide (NF- κ B inhibitor) was lower in high-risk group ( P = 2.6×10 −12 and 2.7×10 −7 , respectively). LPR/GERD may shorten the OS of HNSCC, especially LSCC. The most important finding from this study is that, the HNSCC patients suffering from LPR/GERD benefit less from ICIs.
{"title":"Reflux May Weaken Therapeutic Effect of Immune Checkpoint Inhibitors on Head and Neck Squamous Cell Carcinoma: A Proposed Risk Prediction Model Based on LPR/GERD-Related Genes","authors":"Jun Ju, Jia Wang, Chun Zhang, Zhi Liu","doi":"10.1166/jbn.2023.3682","DOIUrl":"https://doi.org/10.1166/jbn.2023.3682","url":null,"abstract":"Laryngopharyngeal reflux (LPR) and gastroesophageal reflux disease (GERD) involve in head and neck diseases. Their association with carcinogenesis of head and neck squamous cell carcinoma (HNSCC) has attracted increasing attention in recent years. We established a risk prediction model based on LPR/GERD-related genes (RRGs), to investigate the relationship between LPR/GERD and HNSCC, and explore their relevant mechanisms. To establish the risk model, the TCGA-HNSC project from the Cancer Genome Atlas (TCGA) database was used to conduct training and internal validation assays, and the GSE65858 project and five other TCGA projects involving different kinds of tumors were used to conduct the external validation assays. Twelve RRGs were determined to construct risk signature. Kaplan–Meier curves and receiver operating characteristic curves for the model were used to predict 1-, 3-, and 5-year overall survival (OS), particularly for patients with laryngeal squamous cell carcinoma (LSCC). The proportions of immune cells in high-risk group, including T cells, CD8+ T cells, NK cells, myeloid dendritic cells, B lineage, and monocytic lineage, were significantly lower ( P <0.05). Interestingly, risk score was negatively associated with immune checkpoint-related genes expression. The predicted therapeutic sensitivity of immune checkpoint inhibitors (ICIs) in high-risk group was lower (7.27±1.08 vs. 7.80±1.12, P = 4.6×10 −6 ). Moreover, the predicted IC50 of Erlotinib (EGFR inhibitor) and Parthenolide (NF- κ B inhibitor) was lower in high-risk group ( P = 2.6×10 −12 and 2.7×10 −7 , respectively). LPR/GERD may shorten the OS of HNSCC, especially LSCC. The most important finding from this study is that, the HNSCC patients suffering from LPR/GERD benefit less from ICIs.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We investigated the impact of RanBP9 on cell cycle progression and apoptosis in gastric cancer cells. RanBP9 expression was analyzed in 38 clinical gastric cancer tissues using Western blotting. Lentiviral transfection was utilized to establish GES-1 gastric cancer cell models that either overexpressed or silenced RanBP9. Cell proliferation and apoptosis were assessed using MTT and TUNEL staining assays, respectively. Apoptosis-related factors were analyzed by Western blotting and qRT-PCR. Flow cytometry and qRT-PCR were employed to evaluate cell cycle progression and the mRNA levels of CDK4/CyclinD1. The PTEN/PI3K/AKT signaling pathway was examined by Western blotting. We observed a significant reduction in RanBP9 expression in gastric cancer tissues. Overexpression of RanBP9 in GES-1 cells suppressed cell activity, enhanced apoptosis, increased Caspase3 expression and the Bax/Bcl-2 ratio, and decreased CDK4 and CyclinD1 expression, thereby preventing S phase entry. Conversely, knockdown of RanBP9 yielded opposite results. Furthermore, we found that RanBP9 negatively regulated the PTEN/PI3K/AKT pathway. Our findings demonstrate low expression of RanBP9 in gastric cancer tissues and cell lines. We have also established that RanBP9 negatively regulates the PTEN/PI3K/AKT pathway, resulting in cell cycle prolongation and promotion of apoptosis in GES-1 cells.
{"title":"Ras-Related Nuclear Protein-Binding Protein 9 Regulates Gastric Cancer Cell Cycle and Apoptosis Through the PTEN/PI3K/AKT Signaling Pathway","authors":"Meihua Huang, Qiansheng Chen, Yong Fan, Quanhuii Liao, Bingkai Xie, Shunqing Chen, Shurong Li, Wei Liang, Xiaoling Zheng","doi":"10.1166/jbn.2023.3678","DOIUrl":"https://doi.org/10.1166/jbn.2023.3678","url":null,"abstract":"We investigated the impact of RanBP9 on cell cycle progression and apoptosis in gastric cancer cells. RanBP9 expression was analyzed in 38 clinical gastric cancer tissues using Western blotting. Lentiviral transfection was utilized to establish GES-1 gastric cancer cell models that either overexpressed or silenced RanBP9. Cell proliferation and apoptosis were assessed using MTT and TUNEL staining assays, respectively. Apoptosis-related factors were analyzed by Western blotting and qRT-PCR. Flow cytometry and qRT-PCR were employed to evaluate cell cycle progression and the mRNA levels of CDK4/CyclinD1. The PTEN/PI3K/AKT signaling pathway was examined by Western blotting. We observed a significant reduction in RanBP9 expression in gastric cancer tissues. Overexpression of RanBP9 in GES-1 cells suppressed cell activity, enhanced apoptosis, increased Caspase3 expression and the Bax/Bcl-2 ratio, and decreased CDK4 and CyclinD1 expression, thereby preventing S phase entry. Conversely, knockdown of RanBP9 yielded opposite results. Furthermore, we found that RanBP9 negatively regulated the PTEN/PI3K/AKT pathway. Our findings demonstrate low expression of RanBP9 in gastric cancer tissues and cell lines. We have also established that RanBP9 negatively regulates the PTEN/PI3K/AKT pathway, resulting in cell cycle prolongation and promotion of apoptosis in GES-1 cells.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Rajaduraipandian, E. Amutha, G. Sabeena, M. Ponnani Kaja Mideen, A. Mercy, S. Gandhimathi, G. Annadurai, R. Mariselvam, Tahani Awad Alahmadi, Sulaiman Ali Alharbi
The work intended to evaluate the potential wound healing properties of TiO 2 doped CeO nanoparticles with the assistance of Rhinacanthus nasutus . Furthermore, an assessment was conducted on the nanoparticles to determine their antioxidant, cytotoxic, anti-diabetic, and anti-inflammatory inhibitory properties, as well as their toxicity in albino rats. The nanoparticles were synthesized in the green method and subjected to characterization through various methods including UV-visible spectroscopy, SEM for morphological study, FTIR to identify functional groups, XRD, and for elemental analysis EDAX. The morphology of the observed nanoparticles were predominantly spherical, exhibiting an agglomerated structure. The findings indicated that approximately 49% of the nanoparticles exhibited DPPH antioxidant activity, as determined by an IC50 value of 2.8 g/mL. The nanoparticles exhibited cytotoxicity in the brine shrimp lethality assay when administered at a concentration of 50 g/mL. Additionally, they displayed notable inhibitory activity against-amylase, with an IC50 value of 2.981 g/mL. The Ames test yielded negative results, suggesting that the nanoparticles exhibited non-toxic properties. In general, the study substantiated the prospective biological uses of TiO 2 -doped CeO nanoparticles.
{"title":"Multifunctional Biocompatible <i>Rhinacanthus nasutus</i> Based TiO<sub>2</sub>-Doped CeO Nanoparticles Synthesis and their Biomedical Evaluations; <i>In-Vitro/In-Vivo</i> Approach","authors":"S. Rajaduraipandian, E. Amutha, G. Sabeena, M. Ponnani Kaja Mideen, A. Mercy, S. Gandhimathi, G. Annadurai, R. Mariselvam, Tahani Awad Alahmadi, Sulaiman Ali Alharbi","doi":"10.1166/jbn.2023.3680","DOIUrl":"https://doi.org/10.1166/jbn.2023.3680","url":null,"abstract":"The work intended to evaluate the potential wound healing properties of TiO 2 doped CeO nanoparticles with the assistance of Rhinacanthus nasutus . Furthermore, an assessment was conducted on the nanoparticles to determine their antioxidant, cytotoxic, anti-diabetic, and anti-inflammatory inhibitory properties, as well as their toxicity in albino rats. The nanoparticles were synthesized in the green method and subjected to characterization through various methods including UV-visible spectroscopy, SEM for morphological study, FTIR to identify functional groups, XRD, and for elemental analysis EDAX. The morphology of the observed nanoparticles were predominantly spherical, exhibiting an agglomerated structure. The findings indicated that approximately 49% of the nanoparticles exhibited DPPH antioxidant activity, as determined by an IC50 value of 2.8 g/mL. The nanoparticles exhibited cytotoxicity in the brine shrimp lethality assay when administered at a concentration of 50 g/mL. Additionally, they displayed notable inhibitory activity against-amylase, with an IC50 value of 2.981 g/mL. The Ames test yielded negative results, suggesting that the nanoparticles exhibited non-toxic properties. In general, the study substantiated the prospective biological uses of TiO 2 -doped CeO nanoparticles.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sheng Yang, Chong Liu, Xiaowen Ji, Xuemin Chen, Yun Wang, Rong Tao
Liver disease is one of the most significant diseases in humans. Various factors can lead to liver degeneration and necrosis, fatty liver, cirrhosis, etc. This study evaluated the diagnostic value of circulating miRNAs in liver injury and examined the role of gold nanorods (AuNRs) in the detection of biological indicators. We collected data from patients with liver injury in three groups. Healthy volunteers were used as the control group, and the levels of serum markers of liver injury were detected in all participants. The circulating miRNAs associated with liver injury were screened by searching the database, and circulating miRNAs were detected by qRT-PCR. The sensitivity and specificity of circulating miRNAs in diagnosing liver injury were analyzed using receiver operating characteristic curve. AuNRs were prepared to evaluate the concentrations of glucose, cholesterol, uric acid, and alkaline phosphatase (ALP), which were compared with the test results of the kit. We found that the levels of alanine aminotransferase and aspartate aminotransferase changed significantly in patients with drug-induced liver injury (DILI). Total bilirubin levels increased significantly in patients with both non-alcoholic steatohepatitis (NASH) and DILI, but difference between the two groups was insignificant. MDH and adenosine deaminase levels increased in patients with liver injury, whereas paraoxonase-1 levels decreased, which can be combined with alanine aminotransferase levels to diagnose patients with liver injury. Compared with the control group, circulating miR-106-5p, miR-194-5p, and miR-29b-3p were specifically expressed in tissues with liver injury: miR-106-5p expression was lowest in NASH, miR-194-5p expression was highest in chronic hepatitis B (CHB) and NASH, and miR-29b-3p expression was highest in CHB. Receiver operating characteristic curve analysis revealed that miR-106-5p, miR-194-5p, and miR-29b-3p can be used to diagnose patients with NASH, DILI, and CHB, respectively. The combination of miR-194-5p and miR-29b-3p showed good diagnostic ability. The results of biological indicator detection using AuNRs were similar to those detected by the kit. Therefore, AuNRs are a promising candidate for biological detection. This study validated the diagnostic value of circulating miRNAs in liver disease and the application of AuNRs in the detection of biological indicators, providing more theoretical basis for the role of circulating miRNAs in disease prevention and diagnosis.
{"title":"The Role of Gold Nanorods in Detecting Circulating microRNAs as Biomarkers in Liver Diseases","authors":"Sheng Yang, Chong Liu, Xiaowen Ji, Xuemin Chen, Yun Wang, Rong Tao","doi":"10.1166/jbn.2023.3676","DOIUrl":"https://doi.org/10.1166/jbn.2023.3676","url":null,"abstract":"Liver disease is one of the most significant diseases in humans. Various factors can lead to liver degeneration and necrosis, fatty liver, cirrhosis, etc. This study evaluated the diagnostic value of circulating miRNAs in liver injury and examined the role of gold nanorods (AuNRs) in the detection of biological indicators. We collected data from patients with liver injury in three groups. Healthy volunteers were used as the control group, and the levels of serum markers of liver injury were detected in all participants. The circulating miRNAs associated with liver injury were screened by searching the database, and circulating miRNAs were detected by qRT-PCR. The sensitivity and specificity of circulating miRNAs in diagnosing liver injury were analyzed using receiver operating characteristic curve. AuNRs were prepared to evaluate the concentrations of glucose, cholesterol, uric acid, and alkaline phosphatase (ALP), which were compared with the test results of the kit. We found that the levels of alanine aminotransferase and aspartate aminotransferase changed significantly in patients with drug-induced liver injury (DILI). Total bilirubin levels increased significantly in patients with both non-alcoholic steatohepatitis (NASH) and DILI, but difference between the two groups was insignificant. MDH and adenosine deaminase levels increased in patients with liver injury, whereas paraoxonase-1 levels decreased, which can be combined with alanine aminotransferase levels to diagnose patients with liver injury. Compared with the control group, circulating miR-106-5p, miR-194-5p, and miR-29b-3p were specifically expressed in tissues with liver injury: miR-106-5p expression was lowest in NASH, miR-194-5p expression was highest in chronic hepatitis B (CHB) and NASH, and miR-29b-3p expression was highest in CHB. Receiver operating characteristic curve analysis revealed that miR-106-5p, miR-194-5p, and miR-29b-3p can be used to diagnose patients with NASH, DILI, and CHB, respectively. The combination of miR-194-5p and miR-29b-3p showed good diagnostic ability. The results of biological indicator detection using AuNRs were similar to those detected by the kit. Therefore, AuNRs are a promising candidate for biological detection. This study validated the diagnostic value of circulating miRNAs in liver disease and the application of AuNRs in the detection of biological indicators, providing more theoretical basis for the role of circulating miRNAs in disease prevention and diagnosis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Current study aims to explore the ameliorative effects of ultrasound in combination with P-selectin-targeted/AM1241-loaded microbubbles (AM1241 MBs) in rats with skeletal muscle ischemia/reperfusion (I/R) injury. AM1241 MB was prepared via rotary evaporation and mechanical shaking. Histological lesions and cellular apoptosis in skeletal muscle were detected by H&E staining and TUNEL staining, respectively. Changes in antioxidant capacity and inflammatory response in skeletal muscle tissue were analyzed by ELISA method. Changes of Nfr2/HO-1 pathway-related proteins were measured via the western blot. As a result, ultrasound-mediated destruction of AM1241 MBs significantly improved the degree of damage and edema in skeletal muscle tissue of rats with I/R injury. Inflammatory response and cellular apoptosis in skeletal muscle tissue were also significantly improved, while the antioxidant capacity was significantly enhanced. Western blot analysis exhibited that ultrasound in combination with AM1241 MBs treatment activated the Nrf2/HO-1 pathway. Nevertheless, selective knockdown of Nrf2 significantly reversed the beneficial effects of ultrasound combined with AM1241 MBs treatment on I/R injuries of the skeletal muscle. Ultrasound-mediated disruption of AM1241 MBs could effectively ameliorate the I/R injuries of skeletal muscle tissues in rats via activating the Nrf2/HO-1 pathway.
{"title":"Ultrasound-Mediated Destruction of P-Selectin-Targeted and Cannabinoid Type 2 Receptor Agonist-Loading Microbubbles Ameliorates Skeletal Muscle Ischemia-Reperfusion Injury in Rat","authors":"Lei Zhang, Qi Yang, Wei Wu, Huiyang Wang, Zhichao Ding, Hebin Zhang, Laizhi Zhang, Hao Wu","doi":"10.1166/jbn.2023.3560","DOIUrl":"https://doi.org/10.1166/jbn.2023.3560","url":null,"abstract":"Current study aims to explore the ameliorative effects of ultrasound in combination with P-selectin-targeted/AM1241-loaded microbubbles (AM1241 MBs) in rats with skeletal muscle ischemia/reperfusion (I/R) injury. AM1241 MB was prepared via rotary evaporation and mechanical shaking. Histological lesions and cellular apoptosis in skeletal muscle were detected by H&E staining and TUNEL staining, respectively. Changes in antioxidant capacity and inflammatory response in skeletal muscle tissue were analyzed by ELISA method. Changes of Nfr2/HO-1 pathway-related proteins were measured via the western blot. As a result, ultrasound-mediated destruction of AM1241 MBs significantly improved the degree of damage and edema in skeletal muscle tissue of rats with I/R injury. Inflammatory response and cellular apoptosis in skeletal muscle tissue were also significantly improved, while the antioxidant capacity was significantly enhanced. Western blot analysis exhibited that ultrasound in combination with AM1241 MBs treatment activated the Nrf2/HO-1 pathway. Nevertheless, selective knockdown of Nrf2 significantly reversed the beneficial effects of ultrasound combined with AM1241 MBs treatment on I/R injuries of the skeletal muscle. Ultrasound-mediated disruption of AM1241 MBs could effectively ameliorate the I/R injuries of skeletal muscle tissues in rats via activating the Nrf2/HO-1 pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dequan He, Jiawei Zhang, Youquan Chen, Zhiliang Li
This study evaluated the role and underlying mechanisms of Tanshinone IIA (Tan IIA) in atherosclerosis. C57BL mice (control group) and ApoE mice (model group) were administered a conventional and high-fat diet for 20 weeks. The Tan IIA group was obtained by administering a high-fat diet plus 8 weeks of Tan IIA to other mice for 20 weeks, followed by oil red O staining and lipid examination. RAW264.7 cells were transfected with PPAR α siRNA+Tan IIA to measure their expression. The results showed little change in body weight between the three groups ( P < 0.05). Liver index was significantly increased in the model and Tan IIA groups ( P <0.05). Atherosclerotic plaques, plaque cross-sectional area, human oxidized low-density lipoprotein (ox-LDL), low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) levels, p-NF- κ B, p-IKK α , P-Ikk α / β , TNF- α and IL-1 β levels were significantly increased in the model group and decreased in the Tan IIA group ( P < 0.05). We also noted a decrease in PPAR α , PGC-1 α and ABCA1 in the model group and an increase in the Tan IIA group. NF- κ B expression was increased in the nucleus and decreased in the cytoplasm in the model group, which was reversed by Tan IIA treatment. Tan IIA significantly reduced ox-LDL, LDL-C and TG levels, plaque size and plaque cross-sectional area in atherosclerosis. Tan IIA effectively inhibited NF- κ B, activated the PPAR α /ABCA1 signalling pathway, and reduce inflammatory pathways, thereby improving lipid deposition and acting as an anti-atherosclerotic agent.
{"title":"Tanshinone IIA Alleviates Atherosclerosis Through Inhibition of NF-<i>κ</i>B and PPAR<i>α</i>/ABCA1 Signaling Pathways","authors":"Dequan He, Jiawei Zhang, Youquan Chen, Zhiliang Li","doi":"10.1166/jbn.2023.3689","DOIUrl":"https://doi.org/10.1166/jbn.2023.3689","url":null,"abstract":"This study evaluated the role and underlying mechanisms of Tanshinone IIA (Tan IIA) in atherosclerosis. C57BL mice (control group) and ApoE mice (model group) were administered a conventional and high-fat diet for 20 weeks. The Tan IIA group was obtained by administering a high-fat diet plus 8 weeks of Tan IIA to other mice for 20 weeks, followed by oil red O staining and lipid examination. RAW264.7 cells were transfected with PPAR α siRNA+Tan IIA to measure their expression. The results showed little change in body weight between the three groups ( P < 0.05). Liver index was significantly increased in the model and Tan IIA groups ( P <0.05). Atherosclerotic plaques, plaque cross-sectional area, human oxidized low-density lipoprotein (ox-LDL), low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) levels, p-NF- κ B, p-IKK α , P-Ikk α / β , TNF- α and IL-1 β levels were significantly increased in the model group and decreased in the Tan IIA group ( P < 0.05). We also noted a decrease in PPAR α , PGC-1 α and ABCA1 in the model group and an increase in the Tan IIA group. NF- κ B expression was increased in the nucleus and decreased in the cytoplasm in the model group, which was reversed by Tan IIA treatment. Tan IIA significantly reduced ox-LDL, LDL-C and TG levels, plaque size and plaque cross-sectional area in atherosclerosis. Tan IIA effectively inhibited NF- κ B, activated the PPAR α /ABCA1 signalling pathway, and reduce inflammatory pathways, thereby improving lipid deposition and acting as an anti-atherosclerotic agent.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135708230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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