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Journal of biomedical materials research. Part B, Applied biomaterials最新文献

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The landscape of clinical trials in corneal regeneration: A systematic review of tissue engineering approaches in corneal disease 角膜再生临床试验的前景:角膜疾病组织工程方法的系统回顾。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-20 DOI: 10.1002/jbm.b.35449
Safieh Boroumand, Mahya Rahmani, Faraz Sigaroodi, Camellia Ganjoury, Azim Parandakh, Alireza Bonakdar, Mohammad-Mehdi Khani, Masoud Soleimani

The limited availability of a healthy donor cornea and the incidence of allograft failure led researchers to seek other corneal substitutes via tissue engineering. Exploring the trend of clinical trials of the cornea with the vision of tissue engineering provides an opportunity to reveal future potential corneal substitutes. The results of this clinical trial are beneficial for future study designs to overcome the limitations of current therapeutic approaches. In this study, registered clinical trials of bio-based approaches were reviewed for corneal regeneration on March 22, 2024. Among the 3955 registered trials for the cornea, 392 trials were included in this study, which categorized in three main bio-based scaffolds, stem cells, and bioactive macromolecules. In addition to the acellular cornea and human amniotic membrane, several bio-based materials have been introduced as corneal substrates such as collagen, fibrin, and agarose. However, some synthetic materials have been introduced in recent studies to improve the desired properties of bio-based scaffolds for corneal substitutes. Nevertheless, new insights into corneal regenerative medicine have recently emerged from cell sheets with autologous and allogeneic cell sources. In addition, the future perspective of corneal regeneration is described through a literature review of recent experimental models.

健康供体角膜的有限性和异体移植失败的发生率促使研究人员通过组织工程学寻找其他角膜替代物。以组织工程学的视野探索角膜临床试验的趋势,为揭示未来潜在的角膜替代品提供了机会。这项临床试验的结果有利于未来的研究设计,以克服当前治疗方法的局限性。本研究对 2024 年 3 月 22 日已登记的角膜再生生物方法临床试验进行了审查。在已登记的 3955 项角膜临床试验中,有 392 项被纳入本研究,主要分为生物基支架、干细胞和生物活性大分子三大类。除了无细胞角膜和人羊膜外,还有一些生物基材料被引入作为角膜基质,如胶原蛋白、纤维蛋白和琼脂糖。不过,最近的研究还引入了一些合成材料,以改善角膜替代品生物基支架的理想特性。尽管如此,自体细胞和异体细胞来源的细胞片最近对角膜再生医学有了新的认识。此外,通过对最新实验模型的文献综述,对角膜再生的未来前景进行了描述。
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引用次数: 0
Reactive oxygen-scavenging polydopamine nanoparticle coated 3D nanofibrous scaffolds for improved osteogenesis: Toward an aging in vivo bone regeneration model 活性氧清除聚多巴胺纳米粒子涂层三维纳米纤维支架用于改善骨生成:建立老化体内骨再生模型。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-19 DOI: 10.1002/jbm.b.35456
Jacob Miszuk, Jue Hu, Zhuozhi Wang, Obiora Onyilagha, Hammad Younes, Collin Hill, Alexei V. Tivanski, Zhengtao Zhu, Hongli Sun

Tissue engineered scaffolds aimed at the repair of critical-sized bone defects lack adequate consideration for our aging society. Establishing an effective aged in vitro model that translates to animals is a significant unmet challenge. The in vivo aged environment is complex and highly nuanced, making it difficult to model in the context of bone repair. In this work, 3D nanofibrous scaffolds generated by the thermally-induced self-agglomeration (TISA) technique were functionalized with polydopamine nanoparticles (PD NPs) as a tool to improve drug binding capacity and scavenge reactive oxygen species (ROS), an excessive build-up that dampens the healing process in aged tissues. PD NPs were reduced by ascorbic acid (rPD) to further improve hydrogen peroxide (H2O2) scavenging capabilities, where we hypothesized that these functionalized scaffolds could rescue ROS-affected osteoblastic differentiation in vitro and improve new bone formation in an aged mouse model. rPDs demonstrated improved H2O2 scavenging activity compared to neat PD NPs, although both NP groups rescued the alkaline phosphatase activity (ALP) of MC3T3-E1 cells in presence of H2O2. Additionally, BMP2-induced osteogenic differentiation, both ALP and mineralization, was significantly improved in the presence of PD or rPD NPs on TISA scaffolds. While in vitro data showed favorable results aimed at improving osteogenic differentiation by PD or rPD NPs, in vivo studies did not note similar improvements in ectopic bone formation an aged model, suggesting that further nuance in material design is required to effectively translate to improved in vivo results in aged animal models.

旨在修复临界骨缺损的组织工程支架缺乏对老龄化社会的充分考虑。建立有效的老化体外模型并将其应用于动物是一项尚未解决的重大挑战。体内老化环境非常复杂且细微,因此很难在骨修复中建立模型。在这项研究中,通过热诱导自团聚(TISA)技术生成的三维纳米纤维支架被多多巴胺纳米颗粒(PD NPs)功能化,以此提高药物结合能力并清除活性氧(ROS),因为活性氧的过度积累会抑制老化组织的愈合过程。为了进一步提高过氧化氢(H2O2)清除能力,我们用抗坏血酸(rPD)还原了 PD NPs,并假设这些功能化支架可以挽救受 ROS 影响的体外成骨细胞分化,并改善老化小鼠模型中新骨的形成。与纯 PD NPs 相比,rPDs 提高了 H2O2 清除活性,尽管两组 NP 都能在 H2O2 存在的情况下挽救 MC3T3-E1 细胞的碱性磷酸酶活性(ALP)。此外,在 TISA 支架上有 PD 或 rPD NP 存在的情况下,BMP2 诱导的成骨分化(包括 ALP 和矿化)都得到了显著改善。虽然体外数据显示了 PD 或 rPD NPs 在改善成骨分化方面的良好效果,但体内研究并没有注意到异位骨形成在老化模型中也有类似的改善,这表明要想有效改善老化动物模型的体内结果,还需要在材料设计方面做进一步的细微调整。
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引用次数: 0
Development of a composite using calcined bone powder and silane cross-linked alginate as bone substitute material 使用煅烧骨粉和硅烷交联藻酸盐作为骨替代材料,开发出一种复合材料。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-19 DOI: 10.1002/jbm.b.35457
Shigeo M. Tanaka

Calcined bone is an attractive natural material for use as a bone substitute because of its cost-effectiveness and high biocompatibility, which are comparable to that of synthetic hydroxyapatite. However, the calcination process has significantly weakened the mechanical properties. In this study, a composite of calcined bovine bone powder reinforced with silane cross-linked alginate was prepared to assess its biocompatibility, osteoconductivity, and mechanical compatibility as a bone substitute material. Culture studies with osteoblast-like cells (MC3T3-E1) showed no cytotoxicity toward the composite and exhibited general cell proliferative properties in its presence. In contrast, the composite reduced the alkaline phosphatase activity of osteoblasts but led to significant noncellular apatite deposition on the surface. In addition, quasi-static compression tests of the composite revealed mechanical properties comparable to those of human cancellous bone. The mechanical properties remained stable under wet conditions and did not deteriorate significantly even after 2 weeks of immersion in simulated body fluid at 37°C. The results show that this composite, composed of calcined bone powder and silane cross-linked alginate, is a promising bone substitute material with biocompatibility, osteoconductivity, and mechanical compatibility.

煅烧骨是一种极具吸引力的天然材料,可用作骨替代品,因为它具有成本效益和较高的生物相容性,可与合成羟基磷灰石相媲美。然而,煅烧过程会大大削弱其机械性能。本研究制备了一种煅烧牛骨粉与硅烷交联海藻酸盐增强的复合材料,以评估其作为骨替代材料的生物相容性、骨传导性和机械相容性。对类成骨细胞(MC3T3-E1)的培养研究表明,该复合材料没有细胞毒性,并且在其存在下表现出一般的细胞增殖特性。相反,该复合材料降低了成骨细胞的碱性磷酸酶活性,但却导致表面出现大量非细胞磷灰石沉积。此外,复合材料的准静态压缩测试显示,其机械性能与人体松质骨相当。机械性能在潮湿条件下保持稳定,即使在 37°C 的模拟体液中浸泡 2 周后也没有明显退化。结果表明,这种由煅烧骨粉和硅烷交联海藻酸盐组成的复合材料是一种很有前途的骨替代材料,具有生物相容性、骨传导性和机械相容性。
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引用次数: 0
Development of hyaluronic acid hydrogel containing prednisolone-encapsulated nonphospholipid liposomes for the treatment of rheumatoid arthritis 开发含有泼尼松龙包裹的非磷脂脂质体的透明质酸水凝胶,用于治疗类风湿性关节炎。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-17 DOI: 10.1002/jbm.b.35453
Wei-Bor Tsai, Chin-Ju Chen

Rheumatoid arthritis (RA) requires therapeutic approaches that alleviate symptoms and inhibit the progression of joint damage. Glucocorticoids (GCs) have been a cornerstone of RA treatment, yet their use is often limited by side effects. Recent advancements suggest that liposome-based delivery systems can improve GC biodistribution, minimizing toxicity. This study introduces an innovative tool for RA treatment using prednisone-encapsulated nonphospholipid liposomes (NPLs) in combination with a hyaluronic acid (HA) hydrogel. Our methodology involved incorporating prednisone (PR) with palmitic acid and cholesterol to formulate stable NPLs using a thin-film hydration technique. The synthesized PR-NPLs, characterized by a mean size of 150 nm, demonstrated uniform distribution and higher drug encapsulation in comparison with conventional phospholipid liposomes. In vitro assays revealed that PR-NPL markedly reduced inflammatory responses in macrophages. Additionally, we successfully incorporated PR-NPL into an HA hydrogel, employing a photoinitiated cross-linking process. This novel composite offered modulable PR release, governed by the degree of hydrogel cross-linking. The developed system presents a promising advancement in RA management, especially suited for intraarticular injections. It potentially enables targeted, controlled drug release with a reduced risk of side effects, signifying a significant improvement over existing RA therapies.

类风湿性关节炎(RA)需要能够缓解症状并抑制关节损伤进展的治疗方法。糖皮质激素(GCs)一直是治疗类风湿关节炎的基石,但其使用往往受到副作用的限制。最近的研究进展表明,基于脂质体的给药系统可以改善糖皮质激素的生物分布,将毒性降至最低。本研究采用泼尼松包封的非磷脂脂质体(NPLs)与透明质酸(HA)水凝胶相结合,介绍了一种治疗风湿性关节炎的创新工具。我们的方法是将泼尼松(PR)与棕榈酸和胆固醇结合,利用薄膜水合技术配制出稳定的非磷脂脂质体。合成的 PR-NPLs 平均粒径为 150 nm,与传统磷脂脂质体相比,分布均匀,药物包封率更高。体外实验表明,PR-NPL 能显著降低巨噬细胞的炎症反应。此外,我们还采用光引发交联工艺,成功地将 PR-NPL 融合到了 HA 水凝胶中。这种新型复合材料可根据水凝胶交联程度调节 PR 释放量。所开发的系统为急性髓鞘炎的治疗带来了希望,尤其适用于关节内注射。它有可能实现有针对性的、可控的药物释放,同时降低副作用风险,这标志着与现有的 RA 疗法相比有了重大改进。
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引用次数: 0
A triple growth factor strategy for optimizing bone augmentation in mice 优化小鼠骨质增生的三重生长因子策略。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-12 DOI: 10.1002/jbm.b.35447
Taichi Tenkumo, Rie Koide, Toru Ogawa, Hirofumi Yamaguchi, Shigeki Suzuki, Makiko Miyashita, Keisuke Nakamura, Han Wang, Nobuhiro Yoda, Keiichi Sasaki

With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V). The prepared scaffolds were surgically implanted into the calvarias of C57BL/6JJcl mice, and hard tissue formation was assessed after 10 and 28 days through histological, tomographic, and biochemical analyses. The combination of BMP-2 and IGF-1 induced a greater volume of hard tissue augmentation compared with that of BMP-2 alone, regardless of VEGF supplementation, and these groups had increased levels of cartilage compared with others. The volume of hard tissue formation was greatest in the BIV group. In contrast, the BI + V group exhibited a hard tissue volume similar to that of the BI group. While VEGF and CD31 levels were highest in the BIV group at 10 days, there was no correlation at the same time point between hard tissue formation and the quantity of M2 macrophages. In conclusion, the simultaneous release of BMP-2, IGF-1, and VEGF proved to be effective in promoting bone augmentation.

随着牙科植入治疗成为黄金标准,在植入前进行有效的骨增量治疗的需求日益增长。本研究旨在评估一种整合了骨形态发生蛋白-2(BMP-2)、胰岛素样生长因子1(IGF-1)和血管内皮生长因子(VEGF)这三种关键生长因子的骨增量策略。我们制作了含有 BMP-2、IGF-1 或血管内皮生长因子的胶原支架,并根据其含量将其分为五组:单独的支架;单独的 BMP-2(BMP-2);BMP-2 和 IGF-1(BI);BMP-2、IGF-1 和血管内皮生长因子(BIV);以及 BMP-2 和 IGF-1 并提前释放血管内皮生长因子(BI + V)。将制备好的支架通过手术植入 C57BL/6JJcl 小鼠的小腿,10 天和 28 天后通过组织学、断层扫描和生化分析评估硬组织的形成。与单独使用 BMP-2 相比,无论是否补充 VEGF,BMP-2 和 IGF-1 的组合都能诱导更大体积的硬组织增生。BIV 组的硬组织形成量最大。相比之下,BI + V 组的硬组织体积与 BI 组相似。虽然 BIV 组的血管内皮生长因子和 CD31 水平在 10 天时最高,但在同一时间点,硬组织的形成与 M2 巨噬细胞的数量没有相关性。总之,同时释放 BMP-2、IGF-1 和 VEGF 被证明能有效促进骨增量。
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引用次数: 0
Enhancing electrical conductivity and mechanical properties of decellularized umbilical cord arteries using graphene coatings 利用石墨烯涂层增强脱细胞脐带动脉的导电性和机械性能
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-05 DOI: 10.1002/jbm.b.35448
Fateme Karimi, Morteza Alizadeh, Fateme Sadat Bitaraf, Vahid Shirshahi

Traditional decellularized bioscaffolds possessing intact vascular networks and unique architecture have been extensively studied as conduits for repairing nerve damage. However, they are limited by the absence of electrical conductivity, which is crucial for proper functioning of nervous tissue. This study focuses on investigating decellularized umbilical cord arteries by applying coatings of graphene oxide (GO) and reduced graphene oxide (RGO) to their inner surfaces. This resulted in a homogeneous GO coating that fully covered the internal lumen of the arteries. The results of electrical measurements demonstrated that the conductivity of the scaffolds could be significantly enhanced by incorporating RGO and GO conductive sheets. At a low frequency of 0.1 Hz, the electrical resistance level of the coated scaffolds decreased by 99.8% with RGO and 98.21% with GO, compared with uncoated scaffolds. Additionally, the mechanical properties of the arteries improved by 24.69% with GO and 32.9% with RGO after the decellularization process. The GO and RGO coatings did not compromise the adhesion of endothelial cells and promoted cell growth. The cytotoxicity tests revealed that cell survival rate increased over time with RGO, while it decreased with GO, indicating the time-dependent effect on the cytotoxicity of GO and RGO. Blood compatibility evaluations showed that graphene nanomaterials did not induce hemolysis but exhibited some tendency toward blood coagulation.

传统的脱细胞生物支架具有完整的血管网络和独特的结构,作为修复神经损伤的管道已被广泛研究。然而,它们因缺乏导电性而受到限制,而导电性对神经组织的正常运作至关重要。本研究通过在脐带动脉内表面涂覆氧化石墨烯(GO)和还原氧化石墨烯(RGO),重点研究了脱细胞脐带动脉。这使得均匀的 GO 涂层完全覆盖了动脉的内腔。电学测量结果表明,加入 RGO 和 GO 导电片后,支架的导电性能显著增强。在 0.1 Hz 的低频下,与未涂覆的支架相比,涂覆了 RGO 的支架的电阻水平降低了 99.8%,涂覆了 GO 的支架的电阻水平降低了 98.21%。此外,脱细胞过程后,GO 和 RGO 的动脉机械性能分别提高了 24.69% 和 32.9%。GO 和 RGO 涂层不会影响内皮细胞的粘附性,并能促进细胞生长。细胞毒性测试表明,随着时间的推移,RGO 的细胞存活率上升,而 GO 的细胞存活率下降,这表明 GO 和 RGO 的细胞毒性受时间影响。血液相容性评估表明,石墨烯纳米材料不会诱发溶血,但有一定的血液凝固倾向。
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引用次数: 0
Chronic exposure to TiO2 micro- and nano particles: A biochemical and histopathological experimental study 长期接触二氧化钛微粒和纳米微粒:生化和组织病理学实验研究
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-05 DOI: 10.1002/jbm.b.35443
Mariela Gisele Domingo, Melisa Kurtz, Guillermo Maglione, Maximiliano Martin, Fernando Brites, Deborah Ruth Tasat, Daniel Gustavo Olmedo

The aim of this work was to analyze the effects of long-term exposure to titanium dioxide (TiO2) micro- (MPs) and nanoparticles (NPs) (six and 12 months) on the biochemical and histopathological response of target organs using a murine model. Male Wistar rats were intraperitoneally injected with a suspension of TiO2 NPs (5 nm; TiO2-NP5 group) or MPs (45 μm; TiO2-NP5 group); the control group was injected with saline solution. Six and 12 months post-injection, titanium (Ti) concentration in plasma and target organs was determined spectrometrically (ICP-MS). Blood smears and organ tissue samples were evaluated by light microscopy. Liver and kidney function was evaluated using serum biochemical parameters. Oxidative metabolism was assessed 6 months post-injection (determination of superoxide anion by nitroblue tetrazolium (NBT) test, superoxide dismutase (SOD) and catalase (CAT), lipid peroxidation, and paraoxonase 1). Titanium (Ti) concentration in target organs and plasma was significantly higher in the TiO2-exposed groups than in the control group. Histological evaluation showed the presence of titanium-based particles in the target organs, which displayed no structural alterations, and in blood monocytes. Oxidative metabolism analysis showed that TiO2 NPs were more reactive over time than MPs (p < .05) and mobilization of antioxidant enzymes and membrane damage varied among the studied organs. Clearance of TiO2 micro and nanoparticles differed among the target organs, and lung clearance was more rapid than clearance from the lungs and kidneys (p < .05). Conversely, Ti concentration in plasma increased with time (p < .05). In conclusion, neither serum biochemical parameters nor oxidative metabolism markers appear to be useful as biomarkers of tissue damage in response to TiO2 micro- and nanoparticle deposits at chronic time points.

本研究的目的是利用小鼠模型分析长期暴露于二氧化钛(TiO2)微颗粒(MPs)和纳米颗粒(NPs)(6 个月和 12 个月)对靶器官生化和组织病理学反应的影响。雄性 Wistar 大鼠腹腔注射 TiO2 NPs(5 纳米;TiO2-NP5 组)或 MPs(45 微米;TiO2-NP5 组)悬浮液;对照组注射生理盐水。注射后 6 个月和 12 个月,血浆和靶器官中的钛(Ti)浓度用光谱法(ICP-MS)测定。用光学显微镜对血液涂片和器官组织样本进行评估。利用血清生化指标对肝肾功能进行评估。对注射后 6 个月的氧化代谢进行了评估(通过硝基蓝四氮唑(NBT)测试测定超氧阴离子、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、脂质过氧化和副氧合酶 1)。目标器官和血浆中的钛(Ti)浓度在二氧化钛暴露组明显高于对照组。组织学评估显示,靶器官和血液单核细胞中存在钛基微粒,但未出现结构性改变。氧化代谢分析表明,随着时间的推移,TiO2 NPs 比 MPs 更具活性(p <.05),抗氧化酶的调动和膜损伤在所研究的器官中各不相同。各目标器官对二氧化钛微粒和纳米粒子的清除率不同,肺部的清除率比肺部和肾脏的清除率更快(p <.05)。相反,血浆中的钛浓度随着时间的推移而增加(p < .05)。总之,无论是血清生化指标还是氧化代谢标志物,似乎都不能作为组织在长期时间点上受到二氧化钛微粒和纳米粒子沉积物损伤的生物标志物。
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引用次数: 0
The effects of submicron-textured surface topography on antibiotic efficacy against biofilms 亚微米纹理表面形貌对生物膜抗生素疗效的影响。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-03 DOI: 10.1002/jbm.b.35436
Asma Khursheed, Li-Chong Xu, Christopher A. Siedlecki

Submicron-textured surfaces have been a promising approach to mitigate biofilm development and control microbial infection. However, the use of the single surface texturing approach is still far from ideal for achieving complete control of microbial infections on implanted biomedical devices. The use of a surface topographic modification that might improve the utility of standard antibiotic therapy could alleviate the complications of biofilms on devices. In this study, we characterized the biofilms of Staphylococcus aureus and Pseudomonas aeruginosa on smooth and submicron-textured polyurethane surfaces after 1, 2, 3, and 7 days, and measured the efficacy of common antibiotics against these biofilms. Results show that the submicron-textured surfaces significantly reduced biofilm formation and growth, and that the efficacy of antibiotics against biofilms grown on textured surfaces was improved compared with smooth surfaces. The antibiotic efficacy appears to be related to the degree of biofilm development. At early time points in biofilm formation, antibiotic treatment reveals reasonably good antibiotic efficacy against biofilms on both smooth and textured surfaces, but as biofilms mature, the efficacy of antibiotics drops dramatically on smooth surfaces, with lesser decreases seen for the textured surfaces. The results demonstrate that surface texturing with submicron patterns is able to improve the use of standard antibiotic therapy to treat device-centered biofilms by slowing the development of the biofilm, thereby offering less resistance to antibiotic delivery to the bacteria within the biofilm community.

亚微米纹理表面是缓解生物膜发展和控制微生物感染的一种很有前景的方法。然而,要完全控制植入式生物医学设备上的微生物感染,使用单一表面纹理方法还远远不够理想。使用表面形貌改良方法可提高标准抗生素疗法的效用,从而减轻生物膜在设备上的并发症。在这项研究中,我们对金黄色葡萄球菌和铜绿假单胞菌在光滑和亚微米纹理聚氨酯表面 1、2、3 和 7 天后形成的生物膜进行了表征,并测量了普通抗生素对这些生物膜的疗效。结果表明,亚微米纹理表面能明显减少生物膜的形成和生长,与光滑表面相比,抗生素对生长在纹理表面上的生物膜的疗效有所提高。抗生素的疗效似乎与生物膜的发展程度有关。在生物膜形成的早期,抗生素治疗对光滑表面和纹理表面上的生物膜都有相当好的疗效,但随着生物膜的成熟,抗生素对光滑表面的疗效急剧下降,对纹理表面的降幅较小。研究结果表明,亚微米图案的表面纹理可以减缓生物膜的发展速度,从而减少生物膜群落中细菌对抗生素的耐药性,从而改善标准抗生素疗法对以设备为中心的生物膜的治疗效果。
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引用次数: 0
TiO2 nanotubes with customized diameters for local drug delivery systems 用于局部给药系统的定制直径二氧化钛纳米管。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-07-01 DOI: 10.1002/jbm.b.35445
Sayaka Miyabe, Yushi Fujinaga, Hiroaki Tsuchiya, Shinji Fujimoto

In this study, we evaluated the drug release behavior of diameter customized TiO2 nanotube layers fabricated by anodization with various applied voltage sequences: conventional constant applied potentials of 20 V (45 nm) and 60 V (80 nm), a 20/60 V stepped potential (50 nm [two-diameter]), and a 20–60 V swept potential (49 nm [full-tapered]) (values in parentheses indicate the inner tube diameter at the top part of nanotube layers). The structures of the 50 nm (two-diameter) and 49 nm (full-tapered) samples had smaller inner diameters at the top part of nanotube layers than that of the 80 nm sample, while the outer diameters at the bottom part of nanotube layers were almost the same size as the 80 nm sample. The 80 nm sample, which had the largest nanotube diameter and length, exhibited the greatest burst release, followed by the 50 nm (two-diameter), 49 nm (full-tapered), and 45 nm samples. The initial burst released drug amounts and release rates from the 50 nm (two-diameter) and 49 nm (full-tapered) samples were significantly suppressed by the smaller tube top. On the other hand, the largest proportion of the slow released drug amount to the total released drug amount was observed for the 50 nm (two-diameter) sample. Thus, 50 nm (two-diameter) achieved suppressed initial burst release and large storage capacity. Therefore, this study has, for the first time, applied TiO2 nanotube layers with modulated diameters (two-diameter and full-tapered) to the realization of a localized drug delivery system (LDDS) with customized drug release properties.

在本研究中,我们评估了通过阳极氧化制造的直径定制 TiO2 纳米管层的药物释放行为,这些纳米管层采用了不同的外加电压序列:20 V(45 nm)和 60 V(80 nm)的传统恒定外加电位、20/60 V 的阶跃电位(50 nm [双直径])和 20-60 V 的扫频电位(49 nm [全锥形])(括号中的值表示纳米管层上部的内管直径)。50 nm(双径)和 49 nm(全锥形)样品的纳米管层上部内径小于 80 nm 样品,而纳米管层下部外径与 80 nm 样品几乎相同。纳米管直径和长度最大的 80 nm 样品的猝灭释放量最大,其次是 50 nm(双径)、49 nm(全锥形)和 45 nm 样品。由于管顶较小,50 nm(双径)和 49 nm(全锥形)样品的初始猝灭释放药物量和释放速率明显受到抑制。另一方面,在 50 纳米(双直径)样品中观察到的缓释药物量占总释放药物量的比例最大。因此,50 nm(双直径)实现了抑制初始猝发释放和较大的存储容量。因此,本研究首次将直径可调(双径和全锥形)的 TiO2 纳米管层用于实现具有定制药物释放特性的局部给药系统(LDDS)。
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引用次数: 0
Development and effect of orodispersible film incorporated with Biosilicate for remineralization of dental enamel subjected to cariogenic and erosive challenge 开发含有生物硅酸盐的口腔可分散膜,并确定其对龋齿和腐蚀性牙釉质再矿化的影响。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-27 DOI: 10.1002/jbm.b.35446
Ayodele Alves Amorim, Eduardo José Soares, Fernanda de Carvalho Panzeri Pires-de-Souza

Objectives

The objective of this in vitro study was to assess the efficiency of incorporating Biosilicate particles (30 and 50 mg) into an experimental orodispersible film and its efficacy in the remineralization process of bovine dental enamel under cariogenic and erosive challenges.

Methods

Ninety-nine intact incisors, devoid of cracks or fractures, yielding 198 samples (6 × 6 × 2 mm) via vestibular sectioning using a low-speed diamond disc under water cooling. After flattening the enamel surface with 600, 1200, and 2000 grit sandpaper, the samples were divided into two groups based on the challenges they underwent: cariogenic (0.1 M lactic acid at pH 5.0) or erosive (0.05 M citric acid solution at pH 2.3). Samples from each challenge were further categorized into 11 groups (n = 9) according to the duration of cariogenic (3, 7, and 14 days) or erosive (3, 7, and 10 days) challenge, along with positive control groups (fragments untreated with challenges and treated with different Biosilicate concentrations) and negative controls (fragments treated with artificial saliva for the same periods established for cariogenic and erosive challenges). Treatments with orodispersible films containing Biosilicate (30 and 50 mg) were administered for 2 min per day for 15 days.

Results

The highest remineralizing potential was observed in samples treated with Biosilicate after 14 days of cariogenic challenge, irrespective of the concentration tested. For samples subjected to erosive challenge, erosion time did not affect Biosilicate's remineralizing potential.

Conclusion

Biosilicate shows promise in terms of remineralizing potential in enamel subjected to cariogenic challenge due to its ability to form hydroxycarbonapatite in mineralized tissues.

研究目的本体外研究的目的是评估将生物硅酸盐颗粒(30 毫克和 50 毫克)加入实验性口腔可分散薄膜的效率及其在致龋和侵蚀挑战下对牛牙釉质再矿化过程的功效:九十九颗完整的门牙,无裂纹或骨折,在水冷条件下使用低速金刚石圆盘进行前庭切片,获得 198 个样本(6 × 6 × 2 毫米)。用 600、1200 和 2000 粗细度的砂纸磨平珐琅质表面后,根据样本所经历的挑战将其分为两组:致龋组(0.1 M 乳酸,pH 值为 5.0)或腐蚀组(0.05 M 柠檬酸溶液,pH 值为 2.3)。根据致龋性(3、7 和 14 天)或侵蚀性(3、7 和 10 天)挑战的持续时间,将每次挑战的样本进一步分为 11 组(n = 9),以及阳性对照组(未经挑战处理并用不同浓度的生物硅酸盐处理的片段)和阴性对照组(用人工唾液处理的片段,处理时间与致龋性和侵蚀性挑战确定的时间相同)。使用含有生物硅酸盐(30 毫克和 50 毫克)的口腔分散膜进行处理,每天 2 分钟,持续 15 天:结果:在经过 14 天的致龋挑战后,使用生物硅酸盐处理的样本具有最高的再矿化潜能,无论测试的浓度是多少。对于受到侵蚀性挑战的样品,侵蚀时间并不影响 Biosilicate 的再矿化潜力:结论:由于生物硅酸盐能在矿化组织中形成羟基碳磷灰石,因此它在龋齿挑战下的珐琅质再矿化潜能方面显示出前景。
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引用次数: 0
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Journal of biomedical materials research. Part B, Applied biomaterials
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