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Porous collagen scaffolds enable endothelial lumen formation in vitro under both static and dynamic growth conditions 多孔胶原支架能在体外静态和动态生长条件下形成内皮腔。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-25 DOI: 10.1002/jbm.b.35444
Lena Rössler, Ramin Nasehi, Nadja Hansen, Sanja Aveic, Horst Fischer

Despite recent advances in the field of tissue engineering, the development of complex tissue-like structures in vitro is compromised by the lack of integration of a functioning vasculature. In this study, we propose a mesoscale three-dimensional (3D) in vitro vascularized connective tissue model and demonstrate its feasibility to prompt the self-assembly of endothelial cells into vessel-like structures. Moreover, we investigate the effect of perfusion on the organization of the cells. For this purpose, primary endothelial cells (HUVECs) and a cell line of human foreskin fibroblasts are cultivated in ECM-like matrices made up of freeze-dried collagen scaffolds permeated with collagen type I hydrogel. A tailored bioreactor is designed to investigate the effect of perfusion on self-organization of HUVECs. Immunofluorescent staining, two-photon microscopy, second-harmonic generation imaging, and scanning electron microscopy are applied to visualize the spatial arrangement of the cells. The analyses reveal the formation of hollow, vessel-like structures of HUVECs in hydrogel-permeated collagen scaffolds under both static and dynamic conditions. In conclusion, we demonstrate the feasibility of a 3D porous collagen scaffolding system that enables and maintains the self-organization of HUVECs into vessel-like structures independent of a dynamic flow.

尽管组织工程领域取得了最新进展,但体外复杂组织样结构的发展因缺乏功能性血管的整合而受到影响。在这项研究中,我们提出了一种中尺度三维体外血管化结缔组织模型,并证明了它在促使内皮细胞自组装成血管样结构方面的可行性。此外,我们还研究了灌注对细胞组织的影响。为此,我们将原代内皮细胞(HUVECs)和人包皮成纤维细胞系培养在类似 ECM 的基质中,该基质由冷冻干燥的胶原支架和 I 型胶原水凝胶组成。为研究灌注对 HUVECs 自组织的影响,设计了一种量身定制的生物反应器。应用免疫荧光染色、双光子显微镜、二次谐波发生成像和扫描电子显微镜观察细胞的空间排列。分析结果显示,在静态和动态条件下,HUVEC 在水凝胶渗透的胶原支架中形成了中空的血管样结构。总之,我们证明了三维多孔胶原支架系统的可行性,该系统能使 HUVEC 自组织成血管样结构,且不受动态流动的影响。
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引用次数: 0
Combined regenerative rehabilitation improves recovery following volumetric muscle loss injury in a rat model 联合再生康复疗法可改善大鼠模型肌肉体积损失损伤后的恢复。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-24 DOI: 10.1002/jbm.b.35438
David Johnson, Connor Tobo, Jeffrey Au, Aakash Nagarapu, Natalia Ziemkiewicz, Hannah Chauvin, Jessica Robinson, Saloni Shringarpure, Jamshid Tadiwala, Julia Brockhouse, Colin A. Flaveny, Koyal Garg

Volumetric muscle loss (VML) injury causes irreversible deficits in muscle mass and function, often resulting in permanent disability. The current standard of care is physical therapy, but it is limited in mitigating functional deficits. We have previously optimized a rehabilitation technique using electrically stimulated eccentric contraction training (EST) that improved muscle mass, strength, and size in VML-injured rats. A biosponge scaffold composed of extracellular matrix proteins has previously enhanced muscle function postVML. This study aimed to determine whether combining a regenerative therapy (i.e., biosponge) with a novel rehabilitation technique (i.e., EST) could enhance recovery in a rat model of VML. A VML defect was created by removing ~20% of muscle mass from the tibialis anterior muscle in adult male Lewis rats. Experimental groups included VML-injured rats treated with biosponge with EST or biosponge alone (n = 6/group). EST was implemented 2 weeks postinjury at 150 Hz and was continued for 4 weeks. A linear increase in eccentric torque over 4 weeks showed the adaptability of the VML-injured muscle to EST. Combining biosponge with EST improved peak isometric torque by ~52% compared with biosponge treatment alone at 6 weeks postinjury. Application of EST increased MyoD gene expression and the percentage of large (>2000 μm2) type 2B myofibers but reduced fibrotic tissue deposition in VML-injured muscles. Together, these changes may provide the basis for improved torque production. This study demonstrates the potential for combined regenerative and rehabilitative therapy to improve muscle recovery following VML.

肌肉体积损失(VML)损伤会导致肌肉质量和功能出现不可逆转的缺陷,通常会造成永久性残疾。目前的治疗标准是物理疗法,但这种疗法在减轻功能障碍方面效果有限。我们之前优化了一种康复技术,即使用电刺激偏心收缩训练(EST)来改善 VML 损伤大鼠的肌肉质量、力量和大小。由细胞外基质蛋白组成的生物海绵支架也曾增强了 VML 后的肌肉功能。本研究旨在确定将再生疗法(即生物海绵)与新型康复技术(即EST)相结合是否能促进 VML 大鼠模型的恢复。通过切除成年雄性 Lewis 大鼠胫骨前肌约 20% 的肌肉质量,形成 VML 缺陷。实验组包括使用含有EST的生物海绵或单独使用生物海绵治疗的VML损伤大鼠(n = 6只/组)。损伤后 2 周开始使用EST,频率为 150 Hz,并持续使用 4 周。偏心力矩在 4 周内的线性增加表明 VML 损伤肌肉对 EST 的适应性。与受伤后 6 周时单独使用生物海绵治疗相比,结合使用生物海绵和EST 可使峰值等长扭矩提高约 52%。EST的应用增加了MyoD基因的表达和大(>2000 μm2)2B型肌纤维的百分比,但减少了VML损伤肌肉中纤维组织的沉积。这些变化共同为提高扭矩提供了基础。这项研究表明,结合再生疗法和康复疗法有可能改善 VML 后的肌肉恢复。
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引用次数: 0
The biological and therapeutic assessment of a P(3HB-co-4HB)-bioactive glass-graphene composite biomaterial for tissue regeneration 用于组织再生的 P(3HB-co-4HB)-生物活性玻璃-石墨烯复合生物材料的生物学和治疗学评估。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-24 DOI: 10.1002/jbm.b.35441
Nik S. A. N. Sharifulden, Lady V. Barrios Silva, Nandin-Erdene Mandakhbayar, Seong-Jin Shin, Hae-Won Kim, Jonathan C. Knowles, Linh T. B. Nguyen, David Y. S. Chau

An ideal wound dressing should create a healing environment that relieves pain, protects against infections, maintains moisture, removes debris, and speeds up wound closure and repair. However, conventional options like gauze often fall short in fulfilling these requirements, especially for chronic or nonhealing wounds. Hence there is a critical need for inventive formulations that offer efficient, cost-effective, and eco-friendly alternatives. This study focuses on assessing the innovative formulation based on a microbial-derived copolymer known as poly(3-hydroxybutyrate-co-4-hydroxybutyrate), P(3HB-co-4HB) bioactive glass and graphene particles, and exploring their biological response in vitro and in vivo—to find the best combination that promotes cell adhesion and enhances wound healing. The formulation optimized at concentration of bioactive glass (1 w/w%) and graphene (0.01 w/w%) showed accelerated degradation and enhanced blood vessel formation. Meanwhile biocompatibility was evaluated using murine osteoblasts, human dermal fibroblasts, and standard cell culture assays, demonstrating no adverse effects after 7 days of culture and well-regulated inflammatory kinetics. Whole thickness skin defect using mice indicated the feasibility of the biocomposites for a faster wound closure and reduced inflammation. Overall, this biocomposite appears promising as an ideal wound dressing material and positively influencing wound healing rates.

理想的伤口敷料应能创造一个愈合环境,缓解疼痛、防止感染、保持湿度、清除碎屑并加速伤口闭合和修复。然而,纱布等传统敷料往往无法满足这些要求,尤其是对于慢性或不愈合的伤口。因此,亟需能提供高效、经济、环保替代品的创新配方。本研究重点评估了基于微生物衍生共聚物聚(3-羟基丁酸-4-羟基丁酸)、P(3HB-co-4HB)生物活性玻璃和石墨烯颗粒的创新配方,并探索了它们在体外和体内的生物反应,以找到促进细胞粘附和增强伤口愈合的最佳组合。在生物活性玻璃(1 w/w%)和石墨烯(0.01 w/w%)浓度下优化的配方显示出加速降解和增强血管形成的效果。同时,使用小鼠成骨细胞、人类真皮成纤维细胞和标准细胞培养实验对生物相容性进行了评估,结果表明培养 7 天后无不良影响,炎症动力学调节良好。使用小鼠进行的全厚度皮肤缺损试验表明,生物复合材料可加快伤口愈合并减少炎症反应。总之,这种生物复合材料有望成为一种理想的伤口敷料,并对伤口愈合率产生积极影响。
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引用次数: 0
Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells 构建柯蒂器官模型,研究硫酸小檗碱对受损听觉细胞的影响。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-24 DOI: 10.1002/jbm.b.35439
Junming Zhang, Li Liu, Rong Shen, Xiangxin Lou

Sensorineural hearing loss (SNHL) is mainly caused by injury or loss of hair cells (HCs) and associated spiral ganglion neurons (SGNs) in the inner ear. At present, there is still no effective treatment for SNHL in clinic. Recently, advances in organoid bring a promising prospect for research and treatment of SNHL. Meanwhile, three-dimensional (3D) printing provides a tremendous opportunity to construct versatile organoids for tissue engineering and regenerative medicine. In this study, gelatin (Gel), sodium alginate (SA), and polyvinyl alcohol (PVA) were used to fabricate biomimetic scaffold through 3D printing. The organ of Corti derived from neonatal mice inner ear was seeded on the PVA/Gel/SA scaffold to construct organ of Corti organoid. Then, the organ of Corti organoid was used to study the potential protective effects of berberine sulfate on neomycin-juried auditory HCs and SGNs. The results showed that the PVA/Gel/SA biomimetic 3D scaffolds had good cytocompatibilities and mechanical properties. The constructed organoid could maintain organ of Corti activity well in vitro. In addition, the injury intervention results showed that berberine sulfate could significantly inhibit neomycin-induced HC and SGN damage. This study suggests that the fabricated organoid is highly biomimetic to the organ of Corti, which may provide an effective model for drug development, cell and gene therapy for SNHL.

感音神经性听力损失(SNHL)主要是由内耳毛细胞(HC)和相关螺旋神经节神经元(SGN)的损伤或缺失引起的。目前,临床上仍没有有效治疗 SNHL 的方法。最近,类器官的发展为 SNHL 的研究和治疗带来了广阔的前景。同时,三维(3D)打印技术为组织工程和再生医学提供了构建多功能类器官的巨大机会。在这项研究中,明胶(Gel)、海藻酸钠(SA)和聚乙烯醇(PVA)被用来通过三维打印制造仿生物支架。将取自新生小鼠内耳的Corti器官播种到PVA/Gel/SA支架上,构建Corti器官模型。然后,利用Corti器官模型研究硫酸小檗碱对新霉素疹听觉HC和SGN的潜在保护作用。结果表明,PVA/凝胶/SA仿生三维支架具有良好的细胞相容性和机械性能。构建的类器官在体外能很好地维持 Corti 器官的活性。此外,损伤干预结果表明,硫酸小檗碱能显著抑制新霉素诱导的 HC 和 SGN 损伤。这项研究表明,所构建的类器官与Corti器官具有高度的生物仿真性,可为SNHL的药物开发、细胞和基因治疗提供有效的模型。
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引用次数: 0
Analysis of the broad-spectrum potential of nitric oxide for antibacterial activity against clinically isolated drug-resistant bacteria 分析一氧化氮对临床分离的耐药细菌的广谱抗菌潜力。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-24 DOI: 10.1002/jbm.b.35442
Lori M. Estes Bright, Manjyot Kaur Chug, Stephen Thompson, Megan Brooks, Elizabeth J. Brisbois, Hitesh Handa

The development of drug-resistant microorganisms is taking a heavy toll on the biomedical world. Clinical infections are costly and becoming increasingly dangerous as bacteria that once responded to standard antibiotic treatment are developing resistance mechanisms that require innovative treatment strategies. Nitric oxide (NO) is a gaseous molecule produced endogenously that has shown potent antibacterial capabilities in numerous research studies. Its multimechanistic antibacterial methods prevent the development of resistance and have shown potential as an alternative to antibiotics. However, there has yet to be a direct comparison study evaluating the antibacterial properties of NO against antibiotic susceptible and antibiotic-resistant clinically isolated bacterial strains. Herein, standardized lab and clinically isolated drug-resistant bacterial strains are compared side-by-side for growth and viability following treatment with NO released from S-nitrosoglutathione (GSNO), an NO donor molecule. Evaluation of growth kinetics revealed complete killing of E. coli lab and clinical strains at 17.5 mM GSNO, though 15 mM displayed >50% killing and significantly reduced metabolic activity, with greater dose dependence for membrane permeability. Clinical P. aeruginosa showed greater susceptibility to GSNO during growth curve studies, but metabolic activity and membrane permeability demonstrated similar effects for 12.5 mM GSNO treatment of lab and clinical strains. MRSA lab and clinical strains exhibited total killing at 17.5 mM treatment, though metabolic activity was decreased, and membrane permeation began at 12.5 mM for both strains. Lastly, both S. epidermidis strains were killed by 15 mM GSNO, with sensitivities in metabolic activity and membrane permeability at 12.5 mM GSNO. The mirrored antibacterial effects seen by the lab and clinical strains of two Gram-negative and two Gram-positive bacteria reveal the translational success of NO as an antibacterial therapy and potential alternative to standard antibiotic treatment.

耐药性微生物的发展给生物医学界带来了巨大损失。临床感染的代价高昂,而且越来越危险,因为曾经对标准抗生素治疗有反应的细菌正在发展出需要创新治疗策略的抗药性机制。一氧化氮(NO)是一种内源性产生的气体分子,在大量研究中显示出强大的抗菌能力。它的多机制抗菌方法可防止抗药性的产生,并显示出作为抗生素替代品的潜力。然而,目前还没有直接的比较研究来评估氮氧化物对抗生素易感菌和抗生素耐药的临床分离细菌菌株的抗菌特性。在本文中,研究人员并列比较了标准化实验室细菌菌株和临床分离的耐药细菌菌株在接受由 S-亚硝基谷胱甘肽(GSNO)(一种 NO 供体分子)释放的 NO 处理后的生长和存活率。生长动力学评估显示,17.5 毫摩尔 GSNO 可完全杀死大肠杆菌实验室菌株和临床菌株,但 15 毫摩尔 GSNO 的杀死率大于 50%,代谢活性显著降低,膜渗透性的剂量依赖性更大。在生长曲线研究中,临床铜绿假单胞菌对 GSNO 表现出更大的敏感性,但代谢活性和膜渗透性在 12.5 毫摩尔 GSNO 处理实验室和临床菌株时表现出相似的效果。MRSA 实验室菌株和临床菌株在 17.5 毫摩尔的处理条件下表现出完全杀灭,但代谢活性降低,两种菌株在 12.5 毫摩尔的处理条件下开始膜渗透。最后,两种表皮葡萄球菌菌株都被 15 毫摩尔的 GSNO 杀死,代谢活性和膜渗透性在 12.5 毫摩尔时敏感。两种革兰氏阴性菌和两种革兰氏阳性菌的实验室菌株和临床菌株的抗菌效果相吻合,揭示了 NO 作为一种抗菌疗法和标准抗生素治疗潜在替代品的成功转化。
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引用次数: 0
Hydroxyapatite synthesis and characterization from waste animal bones and natural sources for biomedical applications 从废弃动物骨骼和天然来源中合成羟基磷灰石并确定其特性,用于生物医学应用。
IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Pub Date : 2024-06-24 DOI: 10.1002/jbm.b.35440
Promise Chinonso Okpe, Oladipo Folorunso, Victor Sunday Aigbodion, Camillus Obayi

Hydroxyapatites (HAps) synthesized from waste animal bones have recently gained attention due to their outstanding properties. This is because there is a need to fabricate scaffolds with desirable mechanical strength, ability to withstand high temperatures, and insoluble in solvents such as water, acetone, ethanol, and isopropyl alcohol. This study is an extensive summary of many articles on the routes of synthesis/preparation of HAp, and the optimum processing parameter, and the biomedical application areas, such as: drug administration, dental implants, bone tissue engineering, orthopedic implant coatings, and tissue regeneration/wound healing. A broad catalog of the synthesis methods (and combination methods), temperature/time, shape/size, and the calcium-to-phosphorous (Ca/P) value of diverse waste animal bone sources were reported. The alkaline hydrolysis method is proposed to be suitable for synthesizing HAp from natural sources due to the technique's ability to produce intrinsic HAp. The method is also preferred to the calcination method owing to the phase transformation that takes place at high temperatures during calcinations. However, calcinations aid in removing impurities and germs during heating at high temperatures. When compared to calcination technique, alkaline hydrolysis method results in crystalline HAp; the higher degree of crystallinity is disadvantageous to HAp bioactivity. In addition, the standardization and removal of impurities and contaminants, thorough biocompatibility to ensure clinical safety of the HAp to the human body, and improvement of the mechanical strength and toughness to match specific requirements for the various biomedical applications are the important areas for future studies.

最近,从废弃动物骨骼中合成的羟基磷灰石(HAps)因其出色的性能而备受关注。这是因为需要制造具有理想机械强度、耐高温能力和不溶于水、丙酮、乙醇和异丙醇等溶剂的支架。本研究广泛总结了有关 HAp 的合成/制备路线、最佳加工参数以及生物医学应用领域(如:给药、牙科植入物、骨组织工程、整形外科植入物涂层和组织再生/伤口愈合)的许多文章。报告广泛介绍了各种废弃动物骨源的合成方法(和组合方法)、温度/时间、形状/大小和钙磷(Ca/P)值。由于碱性水解法能够产生固有的 HAp,因此被认为适合从天然资源中合成 HAp。由于煅烧过程中会在高温下发生相变,该方法也比煅烧法更受欢迎。不过,煅烧有助于在高温加热过程中去除杂质和病菌。与煅烧技术相比,碱性水解法产生的 HAp 呈结晶状;较高的结晶度不利于 HAp 的生物活性。此外,杂质和污染物的标准化和去除、彻底的生物相容性以确保 HAp 对人体的临床安全性,以及提高机械强度和韧性以满足各种生物医学应用的特定要求,都是未来研究的重要领域。
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引用次数: 0
Correction to “Bonding of ceramics to silver-coated titanium—A combined theoretical and experimental study” 更正 "陶瓷与银涂层钛的结合--理论与实验的综合研究"。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-06-14 DOI: 10.1002/jbm.b.35437

Vuorinen, V., Kouhia, R., Könönen, M. & Kivilahti, J. K., Bonding of ceramics to silver-coated titanium—a combined theoretical and experimental study, Journal of Biomedical Materials Research – Part B Applied Biomaterials. 112, 5, e35407. doi:10.1002/jbm.b.35407

In the Conclusions section of this article, the word “not” was missing from this sentence:

“As compared with Au, Pt and Pd, silver does react too strongly with titanium, and thus only one relatively thin intermetallic layer, TiAg, is formed and it does not contain as much Kirkendall voids as the intermetallics of the other biometals.”

This should have read: “As compared with Au, Pt and Pd, silver does not react too strongly with titanium, and thus only one relatively thin intermetallic layer, TiAg, is formed and it does not contain as much Kirkendall voids as the intermetallics of the other biometals.”

We apologize for this error.

Vuorinen, V., Kouhia, R., Könönen, M. & Kivilahti, J. K., Bonding of ceramics to silver-coated titanium-a combined theoretical and experimental study, Journal of Biomedical Materials Research - Part B Applied Biomaterials. 112,5,e35407。doi:10.1002/jbm.b.35407 在这篇文章的结论部分,这句话中缺少了 "不是 "一词:"与金、铂和钯相比,银与钛的反应过于强烈,因此只形成了一个相对较薄的金属间层 TiAg,而且它不像其他生物金属的金属间层那样含有较多的 Kirkendall 空隙。"这句话应为:"与金、铂和钯相比,银与钛的反应过于强烈,因此只形成了一个相对较薄的金属间层 TiAg,而且它不像其他生物金属的金属间层那样含有较多的 Kirkendall 空隙:"与金、铂和钯相比,银与钛的反应不太强烈,因此只形成一个相对较薄的金属间层,即 TiAg,它不像其他生物金属间层那样含有较多的 Kirkendall 空隙。"我们对这一错误表示歉意。
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引用次数: 0
Calcium phosphate graphene and Ti3C2Tx MXene scaffolds with osteogenic and antibacterial properties 具有成骨和抗菌特性的磷酸钙石墨烯和 Ti3C2Tx MXene 支架。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-06-14 DOI: 10.1002/jbm.b.35434
Jason D. Orlando, Li Li, Tej B. Limbu, Chenyun Deng, Michelle E. Wolf, Walker M. Vickery, Fei Yan, Stefanie A. Sydlik

Bioactive degradable scaffolds that facilitate bone healing while fighting off initial bacterial infection have the potential to change established strategies of dealing with traumatic bone injuries. To achieve this a composite material made from calcium phosphate graphene (CaPG), and MXene was synthesized. CaPG was created by functionalizing graphene oxide with phosphate groups in the presence of CaBr with a Lewis acid catalyst. Through this transformation, Ca2+ and PO43− inducerons are released as the material degrades thereby aiding in the process of osteogenesis. The 2D MXene sheets, which have shown to have antibacterial properties, were made by etching the Al from a layered Ti3AlC2 (MAX phase) using HF. The hot-pressed scaffolds made of these materials were designed to combat the possibility of infection during initial surgery and failure of osteogenesis to occur. These two failure modes account for a large percentage of issues that can arise during the treatment of traumatic bone injuries. These scaffolds were able to retain induceron-eluting properties in various weight percentages and bring about osteogenesis with CaPG alone and 2 wt% MXene scaffolds demonstrating increased osteogenic activity as compared to no treatment. Additionally, added MXene provided antibacterial properties that could be seen at as little as 2 wt%. This CaPG and MXene composite provides a possible avenue for developing osteogenic, antibacterial materials for treating bone injuries.

生物活性可降解支架既能促进骨愈合,又能抵御初期细菌感染,有望改变处理创伤性骨损伤的既定策略。为此,我们合成了一种由磷酸钙石墨烯(CaPG)和 MXene 制成的复合材料。CaPG 是在路易斯酸催化剂的作用下,在 CaBr 的存在下通过磷酸基团对氧化石墨烯进行官能化而制成的。通过这种转化,材料降解时会释放出 Ca2+ 和 PO4 3-诱导子,从而有助于成骨过程。二维 MXene 片材具有抗菌特性,是通过使用高频从层状 Ti3AlC2(MAX 相)中蚀刻出 Al 而制成的。由这些材料制成的热压支架旨在应对初期手术中可能出现的感染和成骨失败。在治疗创伤性骨损伤的过程中,这两种失败模式占了很大比例。这些支架能够以不同的重量百分比保持诱导剂洗脱特性,并实现成骨,其中单独的 CaPG 和 2 wt% 的 MXene 支架显示出比不处理时更强的成骨活性。此外,添加的 MXene 还具有抗菌特性,其重量仅为 2 wt%。这种 CaPG 和 MXene 复合材料为开发治疗骨损伤的成骨抗菌材料提供了一种可能的途径。
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引用次数: 0
Recent advancements in natural polymers-based self-healing nano-materials for wound dressing 基于天然聚合物的伤口敷料自愈合纳米材料的最新进展。
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-06-12 DOI: 10.1002/jbm.b.35435
Kumar Anand, Rishi Sharma, Neelima Sharma

The field of wound healing has witnessed remarkable progress in recent years, driven by the pursuit of advanced wound dressings. Traditional dressing materials have limitations like poor biocompatibility, nonbiodegradability, inadequate moisture management, poor breathability, lack of inherent therapeutic properties, and environmental impacts. There is a compelling demand for innovative solutions to transcend the constraints of conventional dressing materials for optimal wound care. In this extensive review, the therapeutic potential of natural polymers as the foundation for the development of self-healing nano-materials, specifically for wound dressing applications, has been elucidated. Natural polymers offer a multitude of advantages, possessing exceptional biocompatibility, biodegradability, and bioactivity. The intricate engineering strategies employed to fabricate these polymers into nanostructures, thereby imparting enhanced mechanical robustness, flexibility, critical for efficacious wound management has been expounded. By harnessing the inherent properties of natural polymers, including chitosan, alginate, collagen, hyaluronic acid, and so on, and integrating the concept of self-healing materials, a comprehensive overview of the cutting-edge research in this emerging field is presented in the review. Furthermore, the inherent self-healing attributes of these materials, wherein they exhibit innate capabilities to autonomously rectify any damage or disruption upon exposure to moisture or body fluids, reducing frequent dressing replacements have also been explored. This review consolidates the existing knowledge landscape, accentuating the benefits and challenges associated with these pioneering materials while concurrently paving the way for future investigations and translational applications in the realm of wound healing.

近年来,在先进伤口敷料的推动下,伤口愈合领域取得了显著进展。传统敷料具有生物相容性差、不可生物降解、湿度管理不足、透气性差、缺乏固有治疗特性以及环境影响等局限性。为了实现最佳的伤口护理效果,人们迫切需要创新的解决方案来突破传统敷料材料的限制。在这篇内容广泛的综述中,我们阐明了天然聚合物的治疗潜力,它是开发自愈合纳米材料的基础,尤其适用于伤口敷料应用。天然聚合物具有多种优势,具有优异的生物相容性、生物可降解性和生物活性。为了将这些聚合物制成纳米结构,从而赋予其更强的机械坚固性和柔韧性,并实现有效的伤口管理,研究人员采用了复杂的工程策略。通过利用壳聚糖、海藻酸、胶原蛋白、透明质酸等天然聚合物的固有特性,并结合自愈合材料的概念,本综述全面概述了这一新兴领域的前沿研究。此外,还探讨了这些材料与生俱来的自愈属性,即在暴露于湿气或体液时,能自主纠正任何损伤或破坏,从而减少频繁更换敷料的次数。本综述整合了现有的知识体系,强调了与这些先锋材料相关的益处和挑战,同时也为伤口愈合领域的未来研究和转化应用铺平了道路。
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引用次数: 0
Effect of femtosecond laser induced surface patterns on the flexural strength of monolithic zirconia 飞秒激光诱导表面图案对整体氧化锆抗弯强度的影响
IF 3.4 4区 医学 Q2 Engineering Pub Date : 2024-05-30 DOI: 10.1002/jbm.b.35432
Yener Okutan, Yasemin Gundogdu, Mustafa Borga Donmez, Huseyin Seker, Hamdi Sukur Kilic

To investigate how patterns generated by femtosecond (fs) laser and femtosecond laser power affect the surface roughness (Ra) and biaxial flexural strength (BFS) of monolithic zirconia. Eighty disk-shaped zirconia specimens were divided into eight subgroups (n = 10): Control (C), airborne-particle abrasion (APA), 400 mW fs laser (spiral [SP(400)], square [SQ(400)], circular [CI(400)]), and 700 mW fs laser ([SP(700)], [SQ(700)], [CI(700)]). Ra values were calculated by using a surface profilometer. One additional specimen per group was analyzed with scanning electron microscopy and x-ray diffractometry. BFS values were obtained by using the piston-on-3-ball test. One-way ANOVA and either Tukey's HSD (BFS) or Tamhane's T2 (Ra) tests were used to evaluate data (α = 0.05). Regardless of the pattern and power, fs laser groups had higher Ra than C and APA, while SP groups had lower Ra than CI and SQ groups (p ≤ 0.004). For each pattern, Ra increased with higher laser power (p < 0.001), while the laser power did not affect the BFS (p ≥ 0.793). CI and SQ groups had lower BFS than the other groups (p ≤ 0.040), whereas SP groups had similar BFS to C and APA (p ≥ 0.430). Fs laser microstructuring with spiral surface pattern increased the Ra without jeopardizing the BFS of zirconia. Thus, this treatment might be an option to roughen tested zirconia.

研究飞秒(fs)激光产生的图案和飞秒激光功率如何影响整体氧化锆的表面粗糙度(Ra)和双轴抗弯强度(BFS)。80 个圆盘状氧化锆试样被分为 8 个亚组(n = 10):对照组 (C)、空气颗粒磨损 (APA)、400 mW fs 激光(螺旋形 [SP(400)]、方形 [SQ(400)]、圆形 [CI(400)])和 700 mW fs 激光([SP(700)]、[SQ(700)]、[CI(700)])。Ra 值使用表面轮廓仪计算。每组还有一个试样用扫描电子显微镜和 X 射线衍射仪进行分析。BFS 值通过活塞-3 球测试获得。采用单因子方差分析和 Tukey's HSD(BFS)或 Tamhane's T2(Ra)检验来评估数据(α = 0.05)。无论采用哪种模式和功率,fs 激光组的 Ra 值都高于 C 组和 APA 组,而 SP 组的 Ra 值则低于 CI 组和 SQ 组(p ≤ 0.004)。对于每种模式,Ra 随激光功率的增加而增加(p
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Journal of biomedical materials research. Part B, Applied biomaterials
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