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Network pharmacology analysis and experimental validation to explore the effect and mechanism of tetramethylpyrazine for spinal cord injury 通过网络药理学分析和实验验证探索四甲基吡嗪治疗脊髓损伤的效果和机制
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-03 DOI: 10.1016/j.jchemneu.2023.102386
Guodong Qi , Shujun Li , Qiong Jiang , Zhijuan Yu , Zhenggang Peng , Qiurui Li , Wei Qi , Mingjun Guo

Objective

To investigate the effect and mechanism of Tetramethylpyrazine (TMP) in treating Spinal Cord Injury (SCI) using network pharmacology analysis and animal experiments.

Methods

This study was based on public databases, including PharmMapper, BATMAN-TCM, and STRING, as well as KEGG pathway analysis and other methods of network pharmacology were used to preliminarily explore the molecular mechanism of TMP in the treatment of SCI. Using a mouse SCI compression injury model, the efficacy of TMP was evaluated, and the expression of predictive targets on the PI3K/AKT and MAPK signaling pathways was measured using Western blotting and q-PCR.

Results

Network pharmacology analysis showed that TMP may exert therapeutic effects through the MAPK and PI3K/AKT signaling pathways. In animal experimental validation studies, it was shown that after treatment with TMP, the hind limb motor function scores and ramp test scores of the TMP-treated mice improved significantly. HE staining showed that after treatment with TMP, cavities decreased, fewer glial cells proliferated, and fewer inflammatory cells infiltrated; Nielsen staining showed less neuronal loss. Western blot studies showed that compared with the model group, expression of RAS, ERK1/2, RAF1, PI3K, and p-AKT proteins in the spinal cord tissue of mice treated with high-dose TMP was significantly lower. Accordingly, q-PCR studies showed that compared with the model group, the expression levels of RAS, ERK1/2, RAF1, PI3K, and p-AKT genes in the spinal cords of mice in the high-dose TMP group were significantly lower.

Conclusion

TMP exhibits a good neuroprotective effect after SCI, which may be related to inhibition of the MAPK and PI3K/AKT signaling pathways.

方法 本研究基于PharmMapper、BATMAN-TCM和STRING等公共数据库,采用KEGG通路分析等网络药理学方法,初步探讨了四甲基吡嗪(TMP)治疗脊髓损伤(SCI)的作用和机制。结果网络药理学分析表明,TMP可能通过MAPK和PI3K/AKT信号通路发挥治疗作用。在动物实验验证研究中,经 TMP 治疗后,小鼠的后肢运动功能评分和斜坡测试评分明显改善。HE 染色显示,使用 TMP 治疗后,空洞减少,胶质细胞增生减少,炎症细胞浸润减少;Nielsen 染色显示神经元丢失减少。Western 印迹研究显示,与模型组相比,大剂量 TMP 治疗小鼠脊髓组织中 RAS、ERK1/2、RAF1、PI3K 和 p-AKT 蛋白的表达明显降低。相应地,q-PCR 研究表明,与模型组相比,大剂量 TMP 组小鼠脊髓中 RAS、ERK1/2、RAF1、PI3K 和 p-AKT 基因的表达水平明显降低。
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引用次数: 0
Neurobehavioral deficits, histoarchitectural alterations, parvalbumin neuronal damage and glial activation in the brain of male Wistar rat exposed to Landfill leachate 暴露于垃圾填埋场沥滤液的雄性 Wistar 大鼠脑部的神经行为缺陷、组织结构改变、副发光体神经元损伤和神经胶质激活
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-02 DOI: 10.1016/j.jchemneu.2023.102377
Usende Ifukibot Levi , Mofio M. Bintu , Osinachi Chinonyerem Daniella , Oyelowo-Abdulraheem Fatima Oyenike , Adikpe Oluwa Agbonu , Azeez Mariam Adedamola , Enefe Ndidi , Sanni Fatimah Saka , Beselia V. Gela , Smart I. Mbagwu , Edem Ekpenyong Edem , Olopade James Olukayode , Connor James

Concerns about inappropriate disposal of waste into unsanitary municipal solid waste landfills around the world have been on the increase, and this poses a public health challenge due to leachate production. The neurotoxic effect of Gwagwalada landfill leachate (GLL) was investigated in male adult Wistar rats. Rats were exposed to a 10% concentration of GLL for 21 days. The control group received tap water for the same period of the experiment. Our results showed that neurobehavior, absolute body and brain weights and brain histomorphology as well as parvalbumin interneurons were severely altered, with consequent astrogliosis and microgliosis after 21 days of administrating GLL. Specifically, there was severe loss and shrinkage of Purkinje cells, with their nucleus, and severe diffused vacuolations of the white matter tract of GLL-exposed rat brains. There was severe cell loss in the granular layer of the cerebellum resulting in a reduced thickness of the layer. Also, there was severe loss of dendritic arborization of the Purkinje cells in GLL-exposed rat brains, and damage as well as reduced populations of parvalbumin-containing fast-spiking GABAergic interneurons in various regions of the brain. In conclusion, data from the present study demonstrated the detrimental effects of Gwagwalada landfill leachate on the brain which may be implicated in neuropsychological conditions.

人们对世界各地不适当地将废物丢弃到不卫生的城市固体废物填埋场的担忧与日俱增,而沥滤液的产生给公共卫生带来了挑战。我们以雄性成年 Wistar 大鼠为研究对象,调查了 Gwagwalada 垃圾填埋场沥滤液(GLL)的神经毒性效应。大鼠连续 21 天接触 10%浓度的 GLL。对照组在同一实验期间饮用自来水。我们的结果表明,服用 GLL 21 天后,大鼠的神经行为、绝对体重和脑重、脑组织形态学以及蛛网膜中间神经元都发生了严重改变,并导致星形胶质细胞和小胶质细胞病变。具体来说,暴露于 GLL 的大鼠大脑白质束中的 Purkinje 细胞及其细胞核严重缺失和萎缩,并出现严重的弥漫性空泡。小脑颗粒层细胞严重脱落,导致颗粒层厚度减少。此外,暴露于 GLL 的大鼠大脑中的浦肯野细胞树突轴化也严重丧失,大脑各区域中含有副发光素的快速尖峰 GABA 能中间神经元的数量也受损和减少。总之,本研究的数据证明了瓜瓜拉达垃圾填埋场沥滤液对大脑的有害影响,这可能与神经心理状况有关。
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引用次数: 0
Supplementation with vitamins D3 and a mitigates Parkinsonism in a haloperidol mice model 补充维生素D3和A减轻氟哌啶醇小鼠模型中的帕金森病。
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.jchemneu.2023.102366
Mujittapha Umar Sirajo , John C. Oyem , Mohammed Ibrahim Badamasi

Background

Earlier reports suggest that vitamin D3 (Vit D3) supplementation attenuates Parkinsonism in drug-induced motor deficits. Moreover, the function of Vit D3 may be optimized by co-administration with vitamin A (Vit A). In line with the synergistic interplay between vitamins, we hypothesized that the efficacy of Vit D3 to attenuate Parkinsonism in a haloperidol-induced mouse model of motor deficits would be more potent when concomitantly administered with Vit A.

Methods

Thirty-six (36) adult male mice were randomly divided into six groups of six animals each: the control group, the PD model (haloperidol-treated only group) (-D2), and four other groups treated with haloperidol together with either one or two of the following vitamin supplementations: Vit D3, Vit A, Vit D3 +VA, or bromocriptine a known PD drug respectively. Motor functions were assessed using a battery of neurobehavioral tests in experimental animals, after which brain tissues were harvested and processed for biochemical and histomorphological analysis.

Results

We recorded a significant decline in motor activity in the PD mice model treated with haloperidol alone compared to other experimental groups that received vitamin supplementations. The significant decrease in motor activity observed in the PD mice model corresponded with marked neurodegenerative features in the cytoarchitecture of the pyramidal cells in the striatum and primary motor cortex (M1). Furthermore, the haloperidol-induced PD mice model treated with Vit D3 +Vit A showed significant improvement in motor activity and attenuation of oxidative stress levels and neurodegenerative features compared to other groups treated with Vit A, Vit D3 and bromocriptine alone.

Conclusion

Altogether, our findings suggest that concomitant administration of both Vit D3 and Vit A prevents the development of Parkinsonism features in the haloperidol mouse model of motor deficit. Thus, supplementation with Vit D3 +Vit A may be a viable option for slowing the onset and progression of motor deficits.

背景:早期的报告表明,补充维生素D3 (Vit D3)可减轻药物性运动缺陷的帕金森病。此外,维生素D3与维生素A (Vit A)共给药可能会优化其功能。根据维生素之间的协同相互作用,我们假设维生素D3与维生素A同时给药对氟哌啶醇诱导的运动缺陷小鼠模型中的帕金森病的缓解效果更强。对照组,PD模型(仅氟哌啶醇治疗组)(-D2)和其他四个组,分别使用氟哌啶醇和以下一种或两种维生素补充剂:维生素D3,维生素A,维生素D3+VA或溴硝子汀(已知的PD药物)。通过对实验动物进行一系列神经行为测试来评估运动功能,之后采集脑组织并进行生化和组织形态学分析。结果:我们记录到,与其他接受维生素补充的实验组相比,氟哌啶醇单独治疗的PD小鼠模型的运动活动明显下降。在PD小鼠模型中观察到的运动活动的显著下降与纹状体和初级运动皮层(M1)锥体细胞结构的显著神经退行性特征相一致。此外,与单独用Vit A、Vit D3和溴隐亭治疗的其他组相比,用Vit D3 +Vit A治疗的氟哌啶醇诱导的PD小鼠模型显示出运动活动、氧化应激水平和神经退行性特征的显著改善。结论:总之,我们的研究结果表明,在氟哌啶醇运动缺陷小鼠模型中,同时给予维生素D3和维生素A可以防止帕金森病特征的发展。因此,补充维生素D3+维生素A可能是减缓运动缺陷发生和进展的可行选择。
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引用次数: 0
Icariin attenuates dopaminergic neural loss in haloperidol-induced Parkinsonism in rats via GSK-3β and tyrosine hydroxylase regulation mechanism 淫羊藿苷通过GSK-3β和酪氨酸羟化酶调控机制减轻氟哌啶醇诱导的帕金森病大鼠多巴胺能神经损失
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-30 DOI: 10.1016/j.jchemneu.2023.102385
Hend A. Sabry , Mai M. Zahra

Parkinson’s Disease (PD) is an age-dependent, incessant, dynamic neurodegenerative illness. In animal models, the administration of the dopaminergic D2 antagonist Haloperidol (HP) affects the nigrostriatal pathway, inducing catalepsy, a state of immobility like PD, bradykinesia, and akinesia. The present study investigated the neural effects of Icariin (ICA), a flavonoid derived from Herba Epimedii, against HP-induced PD in rats compared to a standard drug levodopa (L-DOPA). Twenty-four adult male rats were divided into 4 groups: the control group treated with vehicle, the 2nd group treated with HP intraperitoneally, the 3rd group treated with the same dose of HP+L-DOPA orally, and the 4th one, treated with the same dose of HP+ICA orally. All the groups were treated for fourteen consecutive days. Two days before the last dose, locomotor activity was assessed in open field and rotarod tasks. At the end of the experiment, the malondialdehyde, nitric oxide (NO), iron, glycogen synthase kinase-3beta (GSK-3β), and tyrosine hydroxylase (TH) contents, glutathione S-transferase, catalase, superoxide dismutase, activities were estimated in the midbrain. Also, cortex and midbrain monoamine contents (norepinephrine, dopamine, and serotonin) were determined. Moreover, the midbrain histopathology was detected in all treated groups. The results suggested that the neuroleptic effect of HP was completely improved by ICA. This improvement occurred by decreasing the neurotoxicity via lowering midbrain lipid peroxidation, NO, GSK-3β contents, increasing antioxidant biomarkers, TH, and recovering the treated groups' cortex and midbrain monoamines contents. In conclusion, this study suggests that ICA is a suitable treatment for Parkinson's induced by HP.

帕金森病(Parkinson's Disease,PD)是一种与年龄有关的、持续的、动态的神经退行性疾病。在动物模型中,服用多巴胺能 D2 拮抗剂氟哌啶醇(Haloperidol,HP)会影响黑质神经通路,诱发催眠、类似帕金森病的静止状态、运动迟缓和运动障碍。与标准药物左旋多巴(L-DOPA)相比,本研究探讨了淫羊藿中提取的黄酮类化合物淫羊藿苷对HP诱导的帕金森氏症大鼠神经系统的影响。24只成年雄性大鼠被分为4组:对照组用药物治疗,第2组腹腔注射HP,第3组口服相同剂量的HP+L-DOPA,第4组口服相同剂量的HP+ICA。所有组均连续治疗14天。在最后一次给药的前两天,在开阔地和旋转任务中对运动活动进行评估。实验结束时,对中脑的丙二醛、一氧化氮(NO)、铁、糖原合酶激酶-3β(GSK-3β)、酪氨酸羟化酶(TH)含量、谷胱甘肽 S-转移酶、过氧化氢酶、超氧化物歧化酶活性进行了评估。此外,还测定了皮层和中脑单胺(去甲肾上腺素、多巴胺和血清素)的含量。此外,还对所有治疗组的中脑组织病理学进行了检测。结果表明,ICA 完全改善了 HP 的神经抑制作用。这种改善是通过降低中脑脂质过氧化反应、NO、GSK-3β 含量,增加抗氧化生物标志物、TH,以及恢复治疗组的大脑皮层和中脑单胺含量来实现的。总之,本研究表明,ICA 是治疗 HP 诱发的帕金森病的一种合适方法。
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引用次数: 0
Rehabilitation training enhanced the therapeutic effect of calycosin on neurological function recovery of rats following spinal cord injury 康复训练增强了钙苷对脊髓损伤后大鼠神经功能恢复的治疗效果。
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-26 DOI: 10.1016/j.jchemneu.2023.102384
Mingdong Li , Yanqiang Huan , Tianqi Jiang , Yongxiong He , Zengxin Gao

Background

Calycosin (CA), a flavonoids component, has demonstrated potential neuroprotection effects by inhibiting oxidative stress in spinal cord injury (SCI) models. This study aims to investigate the impact of combined rehabilitation training (RT) and calycosin therapy on neurological function following SCI, primarily by assessing changes in motor function recovery, neuronal survival, neuronal oxidative stress levels, and neural proliferation, in order to provide novel insights for the treatment of SCI.

Materials and Methods

The SCI model was constructed by compressing the spinal cord using vascular clamps. Calycosin was injected intraperitoneally into the SCI model rats, and a group of 5 rats underwent RT. The motor function of rats after SCI was evaluated using the Basso Beattle Bresnaha (BBB) score and the inclined plate test. Histopathological changes were evaluated by NeuN immunohistochemistry, HE and Nissl staining. Apoptosis was detected by TUNEL staining. The antioxidant effect of combined treatment was assessed by measuring changes in oxidative stress markers after SCI. Western blot analysis was conducted to examine changes in Hsp90-Akt/ASK1-p38 pathway-related proteins. Finally, cell proliferation was detected by BrdU and Ki67 assays.

Results

RT significantly improved the BBB score and angle of incline promoted by calycosin, resulting in enhanced motor function recovery in rats with SCI. Combining rehabilitation training with calycosin has a positive effect on morphological recovery. Similarly, combined RT enhanced the Nissl and NeuN staining signals of spinal cord neurons increased by calycosin, thereby increasing the number of neurons. TUNEL staining results indicated that calycosin treatment reduced the apoptosis signal in SCI, and the addition of RT further reduced the apoptosis. Moreover, RT combined with calycosin reduced oxidative stress by increasing SOD and GSH levels, while decreasing MDA, NO, ROS, and LDH expressions compared to the calycosin alone. RT slightly enhanced the effect of calycosin in activating Hsp90 and Akt and inhibiting the activation of ASK1 and p38, leading to enhanced inhibition of oxidative stress by calycosin. Additionally, the proliferation indexes (Ki67 and BrdU) assays showed that calycosin treatment alone increased both, whereas the combination treatment further promoted cell proliferation.

Conclusion

Our research findings demonstrate that rehabilitation training enhances the ability of calycosin to reduce oxidative stress, resulting in a decrease in neuronal apoptosis and an increase in proliferation, ultimately promoting neuronal survival.

背景:萼萼素是一种黄酮类成分,在脊髓损伤(SCI)模型中通过抑制氧化应激而显示出潜在的神经保护作用。本研究旨在通过评估运动功能恢复、神经元存活、神经元氧化应激水平和神经增殖等方面的变化,研究康复训练(RT)和萼苷联合疗法对脊髓损伤后神经功能的影响,从而为脊髓损伤的治疗提供新的见解:使用血管钳夹压迫脊髓,构建 SCI 模型。材料和方法:利用血管钳压迫脊髓,构建 SCI 模型,向 SCI 模型大鼠腹腔注射钙磷脂,每 5 只大鼠为一组进行 RT 治疗。使用巴索-贝托-布雷斯纳哈(BBB)评分和斜板试验评估大鼠脊髓损伤后的运动功能。组织病理学变化通过NeuN免疫组化、HE和Nissl染色进行评估。通过 TUNEL 染色检测细胞凋亡。通过测量 SCI 后氧化应激标记物的变化来评估联合治疗的抗氧化效果。进行了 Western 印迹分析,以检测 Hsp90-Akt/ASK1-p38 通路相关蛋白的变化。最后,通过 BrdU 和 Ki67 检测细胞增殖:结果:RT明显改善了BBB评分和钙黄素促进的倾斜角度,从而增强了SCI大鼠的运动功能恢复。康复训练与钙磷脂相结合对形态学恢复有积极作用。同样,联合 RT 可增强钙磷脂增加的脊髓神经元的 Nissl 和 NeuN 染色信号,从而增加神经元的数量。TUNEL 染色结果表明,钙调磷酸治疗可减少 SCI 中的细胞凋亡信号,而 RT 的加入可进一步减少细胞凋亡。此外,与单独使用钙泊三醇相比,RT与钙泊三醇联合使用可提高SOD和GSH水平,降低MDA、NO、ROS和LDH的表达,从而减少氧化应激。RT 稍微增强了钙磷脂激活 Hsp90 和 Akt 的作用,抑制了 ASK1 和 p38 的激活,从而增强了钙磷脂对氧化应激的抑制作用。此外,增殖指标(Ki67和BrdU)检测显示,单独使用钙苷处理可增加细胞增殖,而联合使用可进一步促进细胞增殖:我们的研究结果表明,康复训练能增强钙调素降低氧化应激的能力,从而减少神经元凋亡,增加细胞增殖,最终促进神经元存活。
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引用次数: 0
Electroacupuncture protects against the striatum of ischemia stroke by inhibiting the HMGB1/RAGE/p-JNK signaling pathways 电针通过抑制 HMGB1/RAGE/p-JNK 信号通路保护缺血性脑卒中的纹状体
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-19 DOI: 10.1016/j.jchemneu.2023.102376
Zeyin Nie, Chenying Hu, Huachun Miao, Feng Wu

The striatum (Str) is injured 20 min after permanent ischemic stroke, leading to neurological deficits. Here, we aimed to explore the effect of electroacupuncture (EA) on ischemic stroke and elucidate the possible underlying mechanism. Rat permanent middle cerebral artery occlusion (pMCAO) model, EA treatment, sham-EA (SEA) treatment, beam-balance test, hematoxylin and eosin (HE) staining, Nissl staining, immunofluorescence staining, and Western blot were used to investigate the role of EA in pMCAO. The results showed that balance ability and motor coordination were obviously injured after pMCAO. EA improved balance ability and motor coordination in pMCAO rats. EA reduced striatal injury by reducing the expression of high-mobility group box 1(HMGB1)/receptor for advanced glycation end products (RAGE)/phosphorylated C-Jun N-terminal kinase (p-JNK), whereas SEA did not. Thus, EA plays a neuroprotective role during pMCAO injury, which may be related to the inhibition of HMGB1/RAGE/p-JNK expression.

永久性缺血性脑卒中发生后20分钟,脑纹状体(Str)就会受到损伤,导致神经功能缺损。在此,我们旨在探讨电针(EA)对缺血性脑卒中的影响,并阐明其可能的内在机制。实验采用大鼠永久性大脑中动脉闭塞(pMCAO)模型、EA治疗、假EA(SEA)治疗、横梁平衡试验、苏木精和伊红(HE)染色、Nissl染色、免疫荧光染色、Western blot等方法研究EA在pMCAO中的作用。结果显示,pMCAO后平衡能力和运动协调能力明显受损。EA改善了pMCAO大鼠的平衡能力和运动协调能力。EA通过减少高迁移率组盒1(HMGB1)/高级糖化终产物受体(RAGE)/磷酸化C-Jun N-末端激酶(p-JNK)的表达来减轻纹状体损伤,而SEA则没有。因此,EA在pMCAO损伤中起到神经保护作用,这可能与抑制HMGB1/RAGE/p-JNK的表达有关。
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引用次数: 0
Solifenacin promotes remyelination in cuprizone mouse model by inhibiting the Wnt/β-catenin signaling pathway 索非那新通过抑制 Wnt/β-catenin 信号通路促进铜绿素小鼠模型的髓鞘再形成
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-18 DOI: 10.1016/j.jchemneu.2023.102375
Xinqi Xu , Xueli Song , Fei Chen , Weixing Yan , Qiqi Meng , Jinfeng Liu , Ruiqin Yao , Yaping Liu , Fuxing Dong

Demyelinating diseases are a type of neurological disorder characterized by the damage to the myelin sheath in the central nervous system. Promoting the proliferation and differentiation of oligodendrocyte precursor cells (OPCs) is crucial for treatment. Non-selective muscarinic receptor (MR) antagonists have been shown to improve remyelination in rodent models, although the mechanisms are still unclear. In this study, we treated cuprizone (CPZ)-induced demyelination mouse model with different concentrations of Solifenacin (Sol), a selective M3 receptor antagonist, to determine the optimal concentration for promoting remyelination. Behavioral tests and Luxol fast blue (LFB) staining were used to observe the extent of remyelination, while immunofluorescence was used to measure the expression levels of myelin-related proteins, including myelin basic protein (MBP) and platelet-derived growth factor receptor alpha (PDGFR-α). Western blot analysis was employed to analyze the expression levels of molecules associated with the Wnt/β-catenin signaling pathway. The results showed that Sol treatment significantly promoted myelin regeneration and OPCs differentiation in CPZ-induced demyelination mouse model. Additionally, Sol treatment inhibited the Wnt/β-catenin signaling pathway and reversed the effects of CPZ on OPCs differentiation. In conclusion, Sol may promote the differentiation of OPCs by inhibiting the Wnt/β-catenin signaling pathway, making it a potential therapeutic option for central nervous system demyelinating diseases.

脱髓鞘疾病是一种以神经系统髓鞘受损为特征的神经系统疾病。促进少突胶质前体细胞(OPC)的增殖和分化对治疗至关重要。非选择性毒蕈碱受体(MR)拮抗剂已被证明能改善啮齿类动物模型中的髓鞘再形成,但其机制仍不清楚。在这项研究中,我们用不同浓度的选择性M3受体阻断剂索利芬那辛(Sol)治疗铜绿素(CPZ)小鼠模型,以确定促进髓鞘再形成的最佳浓度。行为测试和卢克索快蓝(LFB)染色用于观察髓鞘再形成的程度,免疫荧光用于测量髓鞘相关蛋白的表达水平,包括髓鞘碱性蛋白(MBP)和血小板衍生生长因子受体α(PDGFRα)。还采用了 Western 印迹分析法来分析与 Wnt/β-catenin 信号通路相关的分子的表达水平。结果表明,在CPZ诱导的小鼠模型中,溶胶处理能明显促进髓鞘再生和OPCs分化。此外,溶胶还能抑制Wnt/β-catenin信号通路,逆转CPZ对OPCs分化的影响。总之,溶胶可通过抑制Wnt/β-catenin信号通路促进OPCs分化,使其成为治疗中枢性脱髓鞘疾病的潜在疗法。
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引用次数: 0
Coenzyme Q10 attenuates neurodegeneration in the cerebellum induced by chronic exposure to tramadol 辅酶Q10减轻慢性曲马多引起的小脑神经退行性变。
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-12-01 DOI: 10.1016/j.jchemneu.2023.102367
Majid Keyhanifard , Roghayeh Javan , Reza Ataee Disfani , Maryam Bahrami , Mohamad Sedigh Mirzaie , Saeid Taghiloo , Hossein Mokhtari , Davood Nasiry , Zahra Sadrzadeh Aghajani , Mahdi Shooraj

Background

Chronic use of tramadol can cause neurotoxic effects and subsequently cause neurodegeneration in the cerebellum. The main damage mechanisms identified are oxidative stress and inflammation. Currently, we investigated the effects of coenzyme Q10 (CoQ10) in attenuates of neurodegeneration in the cerebellum induced by chronic exposure to tramadol.

Material and methods

Seventy-two male mature albino rats were allocated into four equal groups, including; non-treated group, CoQ10 group (which received CoQ10 at 200 mg/kg/day orally for three weeks), tramadol group (which received tramadol hydrochloride at 50 mg/kg/day orally for three weeks), and tramadol+CoQ10 group (which received tramadol and CoQ10 at the same doses as the previous groups). Tissue samples were obtained for stereological, immunohistochemical, biochemical, and molecular evaluations. Also, functional tests were performed to evaluate behavioral properties.

Results

We found a significant increase in stereological parameters, antioxidant factors (catalase, glutathione, and superoxide dismutase), and behavioral function scores in the tramadol+CoQ10 group compared to the tramadol group (p < 0.05). In addition, malondialdehyde levels, the density of apoptotic cells, as well as the expression of pro-inflammatory (tumor necrosis factor-alpha, interleukin 1 beta, and interleukin 6) and autophagy (lysosome-associated membrane protein 2, autophagy-related 5, beclin 1, and autophagy-related 12) genes were considerably reduced in the tramadol+CoQ10 group compared to the tramadol group (p < 0.05).

Conclusion

We conclude that the administration of CoQ10 has neuroprotective effects in the cerebellum of rats that have chronic exposure to tramadol.

背景:长期使用曲马多可引起神经毒性作用,随后引起小脑神经退行性变。确定的主要损伤机制是氧化应激和炎症。目前,我们研究了辅酶Q10 (CoQ10)在慢性曲马多诱导的小脑神经退行性变中的作用。材料与方法:雄性成年白化大鼠72只,随机分为4组:未治疗组,辅酶q10组(口服辅酶q10 200mg/kg/天,持续三周),曲马多组(口服盐酸曲马多50mg/kg/天,持续三周),曲马多+辅酶q10组(曲马多和辅酶q10的剂量与前几组相同)。获得组织样本进行体视学、免疫组织化学、生化和分子评价。此外,还进行了功能测试以评估行为特性。结果:我们发现,与曲马多组相比,曲马多+辅酶q10组的体视学参数、抗氧化因子(过氧化氢酶、谷胱甘肽和超氧化物歧化酶)和行为功能评分显著增加(p结论:我们得出结论,慢性暴露于曲马多的大鼠的小脑中给予辅酶q10具有神经保护作用。
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引用次数: 0
Aqueous leaf extract of Phyllanthus amarus protects against oxidative stress and misfiring of dopaminergic neurons in Paraquat-induced Parkinson’s disease-like model of adult Wistar rats 毛茛叶水提物对百草枯诱导的帕金森病样成年Wistar大鼠氧化应激和多巴胺能神经元失能的保护作用。
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-11-27 DOI: 10.1016/j.jchemneu.2023.102365
Felix U. Enemali, Kingsley Afoke Iteire, Raphael E. Uweigho, Ogunberi Blessing, Gbayisomore Tolulope Judah

Background of the study

Phyllanthus amarus has high nutritional value and is beneficial in managing and treating diverse ailments. This study assessed the role of aqueous leaf extract of Phyllanthus amarus on Paraquat (PQ) induced neurotoxicity in the substantia nigra of Wistar rats.

Materials and methods

The role of aqueous leaves extract of Phyllanthus amarus was assessed using an open field test (OFT) for motor activity, oxidative stress biomarkers [Catalase (CAT), and Superoxide Dismutase (SOD)], histological examination (H and E stained) for cytoarchitectural changes and immunohistochemical studies using tyrosine hydroxylase (TH) as a marker for dopaminergic neurons. Forty-two (42) rats were categorized into six groups (n = 7); group 1: control was administered 0.5 ml/kg distilled water, group 2: received 10 mg/kg PQ + 10 mg/kg L-dopa as reference drug, group 3; received 10 mg/kg PQ, while group 4: received 10 mg/kg PQ + 200 mg/kg P. amarus, group 5: received 10 mg/kg PQ + 300 mg/kg P. amarus, and group 6: received 10 mg/kg PQ + 400 mg/kg P. amarus respectively, for 14 days. All administrations were done orally; a significant difference was set at p < 0.05.

Results and discussion

The study's open field test (OFT) revealed no motor activity deficit with Paraquat (PQ) exposure. Also, cytoarchitectural distortions were not observed with Paraquat (PQ) only treatment group compared to the control and other groups pretreated with P. amarus and L-dopa. Moreover, the Paraquat (PQ) only treatment group showed oxidative stress by significantly decreasing the antioxidant enzyme (SOD) compared to the control and L-dopa pretreated group. A significant decrease in tyrosine hydroxylase (TH) expressing dopaminergic neurons was also observed in Paraquat (PQ) only treatment. However, P. amarus treatment showed therapeutic properties by significantly increasing tyrosine hydroxylase (TH) expressing dopaminergic neuron levels relative to control.

Conclusion

Aqueous leaf extract of Phyllanthus amarus possesses therapeutic properties against Paraquat (PQ) induced changes in the substantia nigra of Wistar rats.

研究背景:毛茛具有很高的营养价值,对多种疾病的管理和治疗有益。本研究评价了毛茛叶水提物对百草枯(PQ)致Wistar大鼠黑质神经毒性的作用。材料和方法:采用开放式实验(OFT)评估毛茛叶水提取物的运动活性,氧化应激生物标志物[过氧化氢酶(CAT)和超氧化物歧化酶(SOD)],组织学检查(H和E染色)检测细胞结构变化,免疫组织化学研究使用酪氨酸羟化酶(TH)作为多巴胺能神经元的标记。42只大鼠分为6组(n = 7);组1:对照组给予0.5ml/kg蒸馏水,组2:给予10mg/kg PQ + 10mg/kg左旋多巴作为对照药,组3;第4组:分别给予10mg/kg PQ + 200mg/kg阿玛兰,第5组:给予10mg/kg PQ + 300mg/kg阿玛兰,第6组:给予10mg/kg PQ + 400mg/kg阿玛兰,连续14天。所有给药均为口服;结果和讨论中有一个显著的差异:该研究的野外测试(OFT)显示百草枯(PQ)暴露后没有运动活动缺陷。此外,与对照组和其他经左旋多巴和紫花草预处理的组相比,仅使用百草枯(PQ)治疗组未观察到细胞结构扭曲。此外,与对照组和左旋多巴预处理组相比,百草枯(PQ)处理组表现出氧化应激,抗氧化酶(SOD)显著降低。在百草枯(PQ)单独处理下,酪氨酸羟化酶(TH)表达多巴胺能神经元显著减少。然而,相对于对照组,野田螺处理通过显著增加酪氨酸羟化酶(TH)表达多巴胺能神经元水平显示出治疗特性。结论:毛茛叶水提物对百草枯(PQ)致Wistar大鼠黑质改变具有治疗作用。
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引用次数: 0
Gallic acid ameliorates behavioral dysfunction, oxidative damage, and neuronal loss in the prefrontal cortex and hippocampus in stressed rats 没食子酸改善应激大鼠前额皮质和海马的行为功能障碍、氧化损伤和神经元丢失。
IF 2.8 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2023-11-26 DOI: 10.1016/j.jchemneu.2023.102364
Gholam Hossein Meftahi , Nahid Aboutaleb

Gallic acid (GA) is known to be a natural phenolic compound with antioxidant and neuroprotective effects. This study aims to investigate the impact of GA against restraint stress-induced oxidative damage, anxiety-like behavior, neuronal loss, and spatial learning and memory impairment in male Wistar rats. The animals were divided into four groups (n = 8) and subjected to restraint stress for 4 h per day for 14 consecutive days or left undisturbed (control without inducing stress). In the treatment group, the animals were treated with 2 mL normal saline plus 100 mg/kg GA per day for 14 consecutive days (STR + GA group). The animals received the drug or normal saline by gavage 2 h before inducing restraint stress. ELISA assay measured oxidative stress factors. Elevated-plus maze and Morris water maze tests assessed anxiety-like behavior and spatial learning and memory, respectively. Also, neuronal density was determined using Nissl staining. Restraint stress significantly increased MDA and reduced the activities of GPX and SOD in the stressed rats, which were reserved by treatment with 100 mg/kg GA. Restraint stress markedly enhanced the anxiety-like behavior and spatial learning and memory impairment that were reserved by GA. In addition, treatment with GA reduced the neuronal loss in the stressed rats in the hippocampus and prefrontal cortex (PFC) regions. Taken together, our findings suggest that GA has the potential to be used as a good candidate to attenuate neurobehavioral disorders as well as neuronal loss in the hippocampus and PFC induced by restraint stress via reducing oxidative damage.

没食子酸(GA)是一种天然酚类化合物,具有抗氧化和神经保护作用。本研究旨在探讨GA对雄性Wistar大鼠应激性氧化损伤、焦虑样行为、神经元丧失和空间学习记忆障碍的影响。将大鼠分为4组(n = 8),分别连续14天每天施加约束应激4小时或不受干扰(对照组不诱导应激)。治疗组给予生理盐水2mL + 100mg/kg GA / d,连续14 d (STR + GA组)。小鼠在诱导约束应激前2h灌胃给药或生理盐水。ELISA法测定氧化应激因子。高架迷宫和莫里斯水迷宫测试分别评估了焦虑样行为和空间学习和记忆。采用尼氏染色法测定神经元密度。抑制应激使应激大鼠MDA含量显著升高,GPX和SOD活性显著降低,而100mg/kg GA对应激大鼠的GPX和SOD活性有保留作用。约束应激显著增强了GA保留的焦虑样行为和空间学习记忆障碍。此外,GA治疗减少了应激大鼠海马和前额叶皮质(PFC)区域的神经元损失。综上所述,我们的研究结果表明,GA有可能通过减少氧化损伤来减轻由约束应激引起的神经行为障碍以及海马和PFC中的神经元损失。
{"title":"Gallic acid ameliorates behavioral dysfunction, oxidative damage, and neuronal loss in the prefrontal cortex and hippocampus in stressed rats","authors":"Gholam Hossein Meftahi ,&nbsp;Nahid Aboutaleb","doi":"10.1016/j.jchemneu.2023.102364","DOIUrl":"10.1016/j.jchemneu.2023.102364","url":null,"abstract":"<div><p>Gallic acid<span><span><span> (GA) is known to be a natural phenolic compound with antioxidant and neuroprotective effects. This study aims to investigate the impact of GA against restraint stress-induced oxidative damage, anxiety-like </span>behavior<span><span><span>, neuronal loss, and spatial learning and memory impairment in male Wistar rats. The animals were divided into four groups (n = 8) and subjected to restraint stress for 4 h per day for 14 consecutive days or left undisturbed (control without inducing stress). In the treatment group, the animals were treated with 2 mL normal saline plus 100 mg/kg GA per day for 14 consecutive days (STR + GA group). The animals received the drug or normal saline by gavage 2 h before inducing restraint stress. </span>ELISA assay measured </span>oxidative stress factors. Elevated-plus maze and </span></span>Morris water maze tests<span><span> assessed anxiety-like behavior and spatial learning and memory, respectively. Also, neuronal density was determined using Nissl staining. Restraint stress significantly increased MDA and reduced the activities of GPX and </span>SOD<span> in the stressed rats, which were reserved by treatment with 100 mg/kg GA. Restraint stress markedly enhanced the anxiety-like behavior and spatial learning and memory impairment that were reserved by GA. In addition, treatment with GA reduced the neuronal loss in the stressed rats in the hippocampus and prefrontal cortex (PFC) regions. Taken together, our findings suggest that GA has the potential to be used as a good candidate to attenuate neurobehavioral disorders as well as neuronal loss in the hippocampus and PFC induced by restraint stress via reducing oxidative damage.</span></span></span></p></div>","PeriodicalId":15324,"journal":{"name":"Journal of chemical neuroanatomy","volume":"134 ","pages":"Article 102364"},"PeriodicalIF":2.8,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of chemical neuroanatomy
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