Pub Date : 2024-10-21DOI: 10.1016/j.jddst.2024.106312
Yogita Ale, Nidhi Nainwal
Exosomes are nanosized extracellular vesicles that influence different facets of cell biology and mediate both short and long-range intercellular communication in health and illness. Exosomes also play important roles in the development of tumours, immunological responses, and neurological diseases. Therefore, it can be utilized as a potential biomarker for diagnosis and prognosis as well as for the treatment of various diseases. Exosomes are regarded as the safest and most selective vehicles for transporting therapeutic molecules to the brain. Exosomes assist in the aggregation, transmission, and efficient elimination of harmful proteins in neurodegenerative diseases. Exosomes are beneficial in the treatment of brain diseases due to their ability to cross the blood-brain barrier (BBB). However, its application to brain diseases as a diagnostic tool and treatment therapy is in the early stages of development. Here, we review recent efforts to deliver drugs via exosomes for the treatment of brain cancer and neurodegenerative diseases, as well as the challenges and potential ways for their clinical application. This article provides a comprehensive discussion of biogenesis, composition, and sources of exosomes as well as techniques for their isolation, purification, characterization, and cargo loading. Various challenges associated with exosomes in the form of scalability, stability, and regulation have been reviewed and the strategies to overcome these challenges are also discussed.
{"title":"Exosomes as nanocarrier for Neurotherapy: Journey from application to challenges","authors":"Yogita Ale, Nidhi Nainwal","doi":"10.1016/j.jddst.2024.106312","DOIUrl":"10.1016/j.jddst.2024.106312","url":null,"abstract":"<div><div>Exosomes are nanosized extracellular vesicles that influence different facets of cell biology and mediate both short and long-range intercellular communication in health and illness. Exosomes also play important roles in the development of tumours, immunological responses, and neurological diseases. Therefore, it can be utilized as a potential biomarker for diagnosis and prognosis as well as for the treatment of various diseases. Exosomes are regarded as the safest and most selective vehicles for transporting therapeutic molecules to the brain. Exosomes assist in the aggregation, transmission, and efficient elimination of harmful proteins in neurodegenerative diseases. Exosomes are beneficial in the treatment of brain diseases due to their ability to cross the blood-brain barrier (BBB). However, its application to brain diseases as a diagnostic tool and treatment therapy is in the early stages of development. Here, we review recent efforts to deliver drugs via exosomes for the treatment of brain cancer and neurodegenerative diseases, as well as the challenges and potential ways for their clinical application. This article provides a comprehensive discussion of biogenesis, composition, and sources of exosomes as well as techniques for their isolation, purification, characterization, and cargo loading. Various challenges associated with exosomes in the form of scalability, stability, and regulation have been reviewed and the strategies to overcome these challenges are also discussed.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-21DOI: 10.1016/j.jddst.2024.106313
Mona M. Agwa , Heba Elmotasem , Sarah A. El-Lakany
Oncological phototherapy has been receiving an immense attention from the medical community for treating malignancies. It merges two interventions of phototherapy approaches, photodynamic and photothermal therapies with chemotherapeutic drug-loaded nanocarriers. This assimilation leverages the safe, non-invasive nature of near irradiated (NIR) light to irradicate the tumor zone, along with the capability of the nanocarriers to passively target the compromised cancer vasculature. Targeting potential is further enhanced through decorating the surface of nanocarriers by targeting ligands allowing the recognition and binding of nanocarriers to tumor cells. This eventually leads to the selective delivery of their chemotherapeutic cargo into the cancerous cells while sparing the healthy ones. Small molecules and peptides are particularly appealing targeting moieties due to their ease of targeting, reduced immunogenicity, and attractive pharmacokinetics properties. This review emphasizes on the current ongoing advancement in the integration of dual phototherapy/chemotherapy with small molecules and peptide-based targeted nanocarriers.
{"title":"Small molecules and peptide ligands directed nano-therapeutics for precise oncological phototherapy: Emphasis towards enhancing chemotherapeutic active tumor targeting efficacy","authors":"Mona M. Agwa , Heba Elmotasem , Sarah A. El-Lakany","doi":"10.1016/j.jddst.2024.106313","DOIUrl":"10.1016/j.jddst.2024.106313","url":null,"abstract":"<div><div>Oncological phototherapy has been receiving an immense attention from the medical community for treating malignancies. It merges two interventions of phototherapy approaches, photodynamic and photothermal therapies with chemotherapeutic drug-loaded nanocarriers. This assimilation leverages the safe, non-invasive nature of near irradiated (NIR) light to irradicate the tumor zone, along with the capability of the nanocarriers to passively target the compromised cancer vasculature. Targeting potential is further enhanced through decorating the surface of nanocarriers by targeting ligands allowing the recognition and binding of nanocarriers to tumor cells. This eventually leads to the selective delivery of their chemotherapeutic cargo into the cancerous cells while sparing the healthy ones. Small molecules and peptides are particularly appealing targeting moieties due to their ease of targeting, reduced immunogenicity, and attractive pharmacokinetics properties. This review emphasizes on the current ongoing advancement in the integration of dual phototherapy/chemotherapy with small molecules and peptide-based targeted nanocarriers.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-21DOI: 10.1016/j.jddst.2024.106314
Yasir Iqbal , Faheem Amin , Mahvish Fatima , Mansoor Khalid , Nosheen Kanwal , Amal A. Abdel Hafez , Ayesha Shuja Farooq , Shams A.M. Issa , Hesham M.H. Zakaly , Manawwer Alam
Chronic wounds impose a significant healthcare burden, demanding effective and biocompatible therapies. Chitosan, a natural biopolymer, holds immense potential for wound healing due to its inherent biocompatibility, hemostatic, and antimicrobial properties. However, incorporating bioactive plant extracts can further enhance its efficacy. Plant extract and natural bioactive compounds have shown the higher capability for tissue regeneration in wound healing applications due to their antibacterial, antioxidant, anti-inflammatory activities and hemostasis. The use of plant extract could be more economical as compared to synthetic drugs as well as non-toxic which can reduce the complication in tissue regeneration in chronic wound healing. However, plant extracts and extracted natural bioactive compounds have shown lower stability, bioavailability and solubility in numerous studies. To overcome these challenges in tissue regeneration applications, numerous forms of carrier are being used such as polymeric nanoparticles, hydrogels, membranes, films, sponges and nanofibers. This review comprehensively explores the anti-inflammatory, pro-angiogenic, and wound healing potential of chitosan-based biomaterials containing plant extracts.
{"title":"Chitosan-based functional materials combined with plant extract: A promising strategy in the stimulation of wound healing process","authors":"Yasir Iqbal , Faheem Amin , Mahvish Fatima , Mansoor Khalid , Nosheen Kanwal , Amal A. Abdel Hafez , Ayesha Shuja Farooq , Shams A.M. Issa , Hesham M.H. Zakaly , Manawwer Alam","doi":"10.1016/j.jddst.2024.106314","DOIUrl":"10.1016/j.jddst.2024.106314","url":null,"abstract":"<div><div>Chronic wounds impose a significant healthcare burden, demanding effective and biocompatible therapies. Chitosan, a natural biopolymer, holds immense potential for wound healing due to its inherent biocompatibility, hemostatic, and antimicrobial properties. However, incorporating bioactive plant extracts can further enhance its efficacy. Plant extract and natural bioactive compounds have shown the higher capability for tissue regeneration in wound healing applications due to their antibacterial, antioxidant, anti-inflammatory activities and hemostasis. The use of plant extract could be more economical as compared to synthetic drugs as well as non-toxic which can reduce the complication in tissue regeneration in chronic wound healing. However, plant extracts and extracted natural bioactive compounds have shown lower stability, bioavailability and solubility in numerous studies. To overcome these challenges in tissue regeneration applications, numerous forms of carrier are being used such as polymeric nanoparticles, hydrogels, membranes, films, sponges and nanofibers. This review comprehensively explores the anti-inflammatory, pro-angiogenic, and wound healing potential of chitosan-based biomaterials containing plant extracts.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-21DOI: 10.1016/j.jddst.2024.106315
Sho'leh Ghaedamini , Mohammad Kazemi , Abbasali Rabiei , Ali Honarvar , Maryam Aliakbari , Saeed Karbasi
Numerous investigations have demonstrated that natural bioactive compounds, such as flavonoids, exhibit synergistic effects with anticancer agents utilized in clinical practice. Stem cell therapies play a critical role in the domain of oncology; nevertheless, conflicting results have surfaced regarding the potential pro-cancer or anti-cancer characteristics of mesenchymal stem cells. The primary aim of the current investigation was to examine the anticancer efficacy of ellagic acid (EA) alone or in combination with adipose tissue stem cells derived conditioned medium (ADSCs-CM) on human breast cancer cells. In order to overcome the potential effects of two-dimensional (2D) culture, a three-dimensional (3D) printed polycaprolactone (PCL)/agarose scaffold is utilized to evaluate cancer cell behavior. More recently it was reported this scaffold had open and interconnected pores, as well as good water absorption and mechanical properties. Assessment of MCF-7 cancer cell viability was conducted through MTT assay, flow cytometry, cell cycle assay, and quantitative real-time PCR within a 3D cell culture framework. The outcomes revealed a reduction in cancer cell viability across all treatment cohorts in comparison to the control group. Particularly noteworthy was the significantly enhanced effect of the EA and CM combination relative to each component administered individually. Flow cytometry assessment using annexin V/PI staining indicated a decrease in cancer cells, particularly evident in the combination-treated group. Furthermore, the mRNA expression levels of BAX, BCL2, vascular endothelial growth factor (VEGF), and caspase 3 genes were consistent with pathways associated with apoptosis. Consequently, the synergistic anti-proliferative impact of EA in conjunction with ADSCs-CM on MCF-7 cells has been substantiated. These findings have two important implications for breast cancer research; highlight the utility of the PCL/agarose scaffold as a cancer model and suggest new avenues for the development of adjuvant anticancer therapeutic approaches.
{"title":"Anti-tumor effects of ellagic acid and mesenchymal stem cell-conditioned medium on breast cancer cells on the 3D printed PCL/agarose scaffolds","authors":"Sho'leh Ghaedamini , Mohammad Kazemi , Abbasali Rabiei , Ali Honarvar , Maryam Aliakbari , Saeed Karbasi","doi":"10.1016/j.jddst.2024.106315","DOIUrl":"10.1016/j.jddst.2024.106315","url":null,"abstract":"<div><div>Numerous investigations have demonstrated that natural bioactive compounds, such as flavonoids, exhibit synergistic effects with anticancer agents utilized in clinical practice. Stem cell therapies play a critical role in the domain of oncology; nevertheless, conflicting results have surfaced regarding the potential pro-cancer or anti-cancer characteristics of mesenchymal stem cells. The primary aim of the current investigation was to examine the anticancer efficacy of ellagic acid (EA) alone or in combination with adipose tissue stem cells derived conditioned medium (ADSCs-CM) on human breast cancer cells. In order to overcome the potential effects of two-dimensional (2D) culture, a three-dimensional (3D) printed polycaprolactone (PCL)/agarose scaffold is utilized to evaluate cancer cell behavior. More recently it was reported this scaffold had open and interconnected pores, as well as good water absorption and mechanical properties. Assessment of MCF-7 cancer cell viability was conducted through MTT assay, flow cytometry, cell cycle assay, and quantitative real-time PCR within a 3D cell culture framework. The outcomes revealed a reduction in cancer cell viability across all treatment cohorts in comparison to the control group. Particularly noteworthy was the significantly enhanced effect of the EA and CM combination relative to each component administered individually. Flow cytometry assessment using annexin V/PI staining indicated a decrease in cancer cells, particularly evident in the combination-treated group. Furthermore, the mRNA expression levels of BAX, BCL2, vascular endothelial growth factor (VEGF), and caspase 3 genes were consistent with pathways associated with apoptosis. Consequently, the synergistic anti-proliferative impact of EA in conjunction with ADSCs-CM on MCF-7 cells has been substantiated. These findings have two important implications for breast cancer research; highlight the utility of the PCL/agarose scaffold as a cancer model and suggest new avenues for the development of adjuvant anticancer therapeutic approaches.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gene delivery is a critical aspect of modern molecular biology and biotechnology, offering promising avenues for therapeutic interventions, disease modeling, and genetic engineering. This review paper provides a comprehensive overview of the recent advancements in gene delivery methodologies, highlighting the latest technologies, strategies, and applications. It outlines the fundamental principles of gene delivery and discusses the challenges associated with current approaches. Subsequently, it explores the innovative strategies that have emerged to overcome those challenges, including viral and non-viral vectors, physical methods, and hybrid approaches. The paper also examines the progress made in the design and engineering of delivery vehicles to enhance targeting, efficiency and safety. Furthermore, it discusses the applications of gene delivery in various fields, such as gene therapy, regenerative medicine, cancer treatment, and vaccine development. It presents case studies and examples of successful gene delivery applications, showcasing the potential impact of these advancements on healthcare and biotechnology. Moreover, the review highlights the emerging trends and future directions in gene delivery research, including the integration of nanotechnology, gene editing techniques like CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats) systems, DNA Tattooing and advancements in delivery route optimization. Finally, it discusses the regulatory and ethical considerations associated with gene delivery technologies and their implications for clinical translation and commercialization. Overall, this review paper provides valuable insights into the recent progress and future prospects of gene delivery, offering researchers, clinicians, and industry professionals a comprehensive understanding of this rapidly evolving field.
基因递送是现代分子生物学和生物技术的一个重要方面，为治疗干预、疾病建模和基因工程提供了前景广阔的途径。本综述全面概述了基因递送方法的最新进展，重点介绍了最新的技术、策略和应用。它概述了基因递送的基本原理，并讨论了与当前方法相关的挑战。随后，它探讨了为克服这些挑战而出现的创新策略，包括病毒和非病毒载体、物理方法和混合方法。论文还探讨了为提高靶向性、效率和安全性而在运载工具的设计和工程方面取得的进展。此外，论文还讨论了基因递送在各个领域的应用，如基因治疗、再生医学、癌症治疗和疫苗开发。报告介绍了成功应用基因递送的案例研究和实例，展示了这些进展对医疗保健和生物技术的潜在影响。此外，综述还强调了基因递送研究的新兴趋势和未来方向，包括纳米技术的整合、CRISPR-Cas（Clustered Regularly Interspaced Short Palindromic Repeats）系统等基因编辑技术、DNA Tattooing 以及递送路径优化方面的进展。最后，论文讨论了与基因递送技术相关的监管和伦理问题，以及这些问题对临床转化和商业化的影响。总之，这篇综述论文为基因递送的最新进展和未来前景提供了有价值的见解，让研究人员、临床医生和行业专业人士对这一快速发展的领域有了全面的了解。
{"title":"Genetic frontiers: Exploring the latest strategies in gene delivery","authors":"Sheikh Shahnawaz Quadir , Devendra Choudhary , Supriya Singh , Deepak Choudhary , Min-Hua Chen , Garima Joshi","doi":"10.1016/j.jddst.2024.106316","DOIUrl":"10.1016/j.jddst.2024.106316","url":null,"abstract":"<div><div>Gene delivery is a critical aspect of modern molecular biology and biotechnology, offering promising avenues for therapeutic interventions, disease modeling, and genetic engineering. This review paper provides a comprehensive overview of the recent advancements in gene delivery methodologies, highlighting the latest technologies, strategies, and applications. It outlines the fundamental principles of gene delivery and discusses the challenges associated with current approaches. Subsequently, it explores the innovative strategies that have emerged to overcome those challenges, including viral and non-viral vectors, physical methods, and hybrid approaches. The paper also examines the progress made in the design and engineering of delivery vehicles to enhance targeting, efficiency and safety. Furthermore, it discusses the applications of gene delivery in various fields, such as gene therapy, regenerative medicine, cancer treatment, and vaccine development. It presents case studies and examples of successful gene delivery applications, showcasing the potential impact of these advancements on healthcare and biotechnology. Moreover, the review highlights the emerging trends and future directions in gene delivery research, including the integration of nanotechnology, gene editing techniques like CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats) systems, DNA Tattooing and advancements in delivery route optimization. Finally, it discusses the regulatory and ethical considerations associated with gene delivery technologies and their implications for clinical translation and commercialization. Overall, this review paper provides valuable insights into the recent progress and future prospects of gene delivery, offering researchers, clinicians, and industry professionals a comprehensive understanding of this rapidly evolving field.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-19DOI: 10.1016/j.jddst.2024.106310
Weimin Jin , Houyuan Yang , Miaomiao Zhang , Zegeng Li , Nianxia Sun , Zhili Han , Chenglin Shu , Zhenbao Li , Dianlei Wang
Inhalation dry powders are in great demand for the treatment of lung diseases due to their unique advantages such as excellent direct delivery, rapid onset of action, and reduced systemic side effects. Herein, this work highlights the successful development of an inhalable paclitaxel dry powder, which is facilely constructed through the optimization of the formulation between chemotherapy components (paclitaxel) and a metal-organic framework (MOF) carrier (γ-cyclodextrin and KOH). Moderate pore and particle size distribution, as well as low hygroscopicity endow the dry powder with excellent loading efficiency and resistance to degradation. All ingredients and their interactions confer favorable emptying rates, flowability, and pulmonary deposition, as well as direct lung drug delivery and release. These attractive characteristics enable the dry powder to effectively enhance cytotoxicity, improve pharmacokinetics, promote lung accumulation, and reduce the side effects of paclitaxel. Thus, this paclitaxel dry powder may open a new avenue for the inhalation treatment of diseases, greatly advancing its clinical application.
{"title":"Inhalable cyclodextrin metal-organic framework dry powder enhanced direct pulmonary delivery of paclitaxel for the treatment of lung cancer","authors":"Weimin Jin , Houyuan Yang , Miaomiao Zhang , Zegeng Li , Nianxia Sun , Zhili Han , Chenglin Shu , Zhenbao Li , Dianlei Wang","doi":"10.1016/j.jddst.2024.106310","DOIUrl":"10.1016/j.jddst.2024.106310","url":null,"abstract":"<div><div>Inhalation dry powders are in great demand for the treatment of lung diseases due to their unique advantages such as excellent direct delivery, rapid onset of action, and reduced systemic side effects. Herein, this work highlights the successful development of an inhalable paclitaxel dry powder, which is facilely constructed through the optimization of the formulation between chemotherapy components (paclitaxel) and a metal-organic framework (MOF) carrier (γ-cyclodextrin and KOH). Moderate pore and particle size distribution, as well as low hygroscopicity endow the dry powder with excellent loading efficiency and resistance to degradation. All ingredients and their interactions confer favorable emptying rates, flowability, and pulmonary deposition, as well as direct lung drug delivery and release. These attractive characteristics enable the dry powder to effectively enhance cytotoxicity, improve pharmacokinetics, promote lung accumulation, and reduce the side effects of paclitaxel. Thus, this paclitaxel dry powder may open a new avenue for the inhalation treatment of diseases, greatly advancing its clinical application.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142525912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The interaction between bacteria and implanted medical device surfaces presents a significant challenge in healthcare. This interaction often leads to biofilm formation, resulting in prolonged bacterial exposure and operational complications. Consequently, the risk of developing multidrug-resistant infections increases, posing a serious threat to patient health and treatment efficacy. Effective prevention of biofilm development requires a comprehensive understanding of the physicochemical properties of biomaterials. Recent advancements in the study of natural antifouling mechanisms have provided valuable insights for developing materials resistant to bacterial colonization. These discoveries offer promising directions for creating more effective antifouling surfaces. However, the existing surface topographies of medical devices, originally designed for optimal tissue integration, may unintentionally facilitate microbial adhesion. This review highlights the crucial need to evaluate the biocompatibility of medical device surfaces, emphasizing the impact of their specific topographical features on bacterial adhesion and biofilm development. We emphasize that surface topography can either promote or inhibit bacterial colonization, depending on specific features such as roughness, pattern, and scale. Understanding these topography-dependent effects is crucial for designing surfaces that minimize bacterial adhesion while maintaining optimal functionality and biocompatibility for the intended medical application. Our analysis reveals significant findings regarding the complex relationship between bacteria and three-dimensional surface properties. This knowledge provides a foundation for further advancements in the development of efficient antifouling materials. By understanding the nuances of bacterial-surface interactions, researchers can design more effective strategies to prevent biofilm formation. Through an extensive examination of preclinical studies, this research not only elucidates the mechanisms of bacterial adhesion but also paves the way for innovative solutions.
{"title":"Microtopographic influence on bacterial biofilm development in habitat-like environments","authors":"Krishna Yadav , Kantrol Kumar Sahu , Sucheta , Sunita Minz , Wasim Raza , Madhulika Pradhan","doi":"10.1016/j.jddst.2024.106311","DOIUrl":"10.1016/j.jddst.2024.106311","url":null,"abstract":"<div><div>The interaction between bacteria and implanted medical device surfaces presents a significant challenge in healthcare. This interaction often leads to biofilm formation, resulting in prolonged bacterial exposure and operational complications. Consequently, the risk of developing multidrug-resistant infections increases, posing a serious threat to patient health and treatment efficacy. Effective prevention of biofilm development requires a comprehensive understanding of the physicochemical properties of biomaterials. Recent advancements in the study of natural antifouling mechanisms have provided valuable insights for developing materials resistant to bacterial colonization. These discoveries offer promising directions for creating more effective antifouling surfaces. However, the existing surface topographies of medical devices, originally designed for optimal tissue integration, may unintentionally facilitate microbial adhesion. This review highlights the crucial need to evaluate the biocompatibility of medical device surfaces, emphasizing the impact of their specific topographical features on bacterial adhesion and biofilm development. We emphasize that surface topography can either promote or inhibit bacterial colonization, depending on specific features such as roughness, pattern, and scale. Understanding these topography-dependent effects is crucial for designing surfaces that minimize bacterial adhesion while maintaining optimal functionality and biocompatibility for the intended medical application. Our analysis reveals significant findings regarding the complex relationship between bacteria and three-dimensional surface properties. This knowledge provides a foundation for further advancements in the development of efficient antifouling materials. By understanding the nuances of bacterial-surface interactions, researchers can design more effective strategies to prevent biofilm formation. Through an extensive examination of preclinical studies, this research not only elucidates the mechanisms of bacterial adhesion but also paves the way for innovative solutions.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-19DOI: 10.1016/j.jddst.2024.106297
Márcio Robert Mattos da Silva , Tereza Cristina Luque Castellane , Bruna Fernanda Silva de Sousa , Eliana Gertrudes de Macedo Lemos , Elisabete Pereira dos Santos , Catarina Amorim Oliveira , Eduardo Ricci-Junior
Contagious diseases caused by mosquito bites are responsible for epidemic outbreaks around the world. Applying mosquito repellents is the main and most cost-effective prophylactic measure to reduce the transmission risk of arboviruses. In this study, two topical gel Formulations were developed as mosquito repellents which use two different systems to release 20 % Icaridin. The first, Formulation 1b uses Pluronic F127®, and the second, Formulation 2b uses a biopolymer produced by bacteria from the Rhizobium tropici species. The repellent Formulations developed showed non-Newtonian behavior. For Formulation 1b the viscosity increased after adding Icaridin. However, for Formulation 2b, there was no increase in viscosity after adding the active substance. The thixotropy analysis showed that the Formulations have good recovery of their structure and adhesion. Adding of Icaridin led to an increase in gel strength in Formulation 1b and did not change the gel strength for Formulation 2b. The Formulations presented very similar occlusion capacities which were all higher than free Icaridin. The Formulations were not cytotoxic in the concentration range between 0.04 % and 0.3 %. In addition, at the 0.62 % concentration, they showed a slight reduction in cell viability, although the LD50 value was not reached. The mean protection time was longer for Formulation 2b compared to Formulation 1b. Both Formulations had a longer mean protection time than free Icaridin against Aedes aegypti. The combination of Icaridin with Pluronic F127® and the biopolymer is an innovation in the pharmaceutical area.
{"title":"Development, characterization and evaluation of in vitro safety and in vivo efficacy of repellent gel formulations","authors":"Márcio Robert Mattos da Silva , Tereza Cristina Luque Castellane , Bruna Fernanda Silva de Sousa , Eliana Gertrudes de Macedo Lemos , Elisabete Pereira dos Santos , Catarina Amorim Oliveira , Eduardo Ricci-Junior","doi":"10.1016/j.jddst.2024.106297","DOIUrl":"10.1016/j.jddst.2024.106297","url":null,"abstract":"<div><div>Contagious diseases caused by mosquito bites are responsible for epidemic outbreaks around the world. Applying mosquito repellents is the main and most cost-effective prophylactic measure to reduce the transmission risk of arboviruses. In this study, two topical gel Formulations were developed as mosquito repellents which use two different systems to release 20 % Icaridin. The first, Formulation 1b uses Pluronic F127®, and the second, Formulation 2b uses a biopolymer produced by bacteria from the <em>Rhizobium tropici</em> species. The repellent Formulations developed showed non-Newtonian behavior. For Formulation 1b the viscosity increased after adding Icaridin. However, for Formulation 2b, there was no increase in viscosity after adding the active substance. The thixotropy analysis showed that the Formulations have good recovery of their structure and adhesion. Adding of Icaridin led to an increase in gel strength in Formulation 1b and did not change the gel strength for Formulation 2b. The Formulations presented very similar occlusion capacities which were all higher than free Icaridin. The Formulations were not cytotoxic in the concentration range between 0.04 % and 0.3 %. In addition, at the 0.62 % concentration, they showed a slight reduction in cell viability, although the LD<sub>50</sub> value was not reached. The mean protection time was longer for Formulation 2b compared to Formulation 1b. Both Formulations had a longer mean protection time than free Icaridin against <em>Aedes aegypti</em>. The combination of Icaridin with Pluronic F127® and the biopolymer is an innovation in the pharmaceutical area.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.jddst.2024.106306
Laura Ferreira , Beatriz Torres , Huma Hameed , Amélia C.F. Vieira , Sachin Kumar Singh , Kamal Dua , Francisco Veiga , Patrícia C. Pires , Priscila Gava Mazzola , Ana Cláudia Paiva-Santos
The exponential increase in screen usage during the COVID-19 pandemic has raised concerns about higher exposure to blue light (BL) from electronic devices and raising concerns about its effects on skin health. BL significantly contributes to skin photoaging, which has aroused considerable interest among researchers due to its proximity in wavelength range to ultraviolet (UV) radiation. Owing to this high exposure and considering the high dynamism of the dermocosmetic market, formulations started to appear directed to combat skin photoaging, stimulating the interest of consumers with marketing strategies and appealing claims. These formulations have the purpose of preventing skin photoaging through the use of UV filters, providing broad UV and BL protection; and incorporation of compounds with mainly antioxidant and photoprotective properties, that allow the mitigation of various damaging effects that can occur as a result of the formation of free radicals, mainly DNA damage, hyperpigmentation, and deregulation of the circadian rhythm. These substances present limitations in solubility and photostability, i.e. high susceptibility to degradation and, consequently, a higher risk of toxicity. To circumvent these issues and maximize the antioxidant potential of these components, nanodelivery systems offer multifunctional and targeted approaches that can significantly enhance their bioavailability, efficacy, and safety. Overall, this review explores the beneficial and adverse effects of BL on the skin, with a focus on the most recent in vivo and in vitro studies of active ingredients, and the application of nanodelivery systems to enhance its performance. The databases used for literature research were Pubmed, Science Direct, and Web of Science, while research on major patent innovations was made through notorious patent databases, such as Patentscope and Espacenet, through the period of 2019–2024. Additionally, this review addresses the sustainability challenges in the development of cosmetic ingredients, highlighting their importance for effective clinical translation and future research.
在 COVID-19 大流行期间，屏幕使用量呈指数级增长，这引起了人们对电子设备蓝光（BL）照射量增加的担忧，以及对蓝光对皮肤健康影响的担忧。蓝光在很大程度上会导致皮肤光老化，由于其波长范围接近紫外线（UV）辐射，这引起了研究人员的极大兴趣。由于这种高度暴露性，并考虑到皮肤化妆品市场的高度活力，开始出现针对皮肤光老化的配方，通过营销策略和吸引人的声称来激发消费者的兴趣。这些配方的目的是通过使用紫外线过滤剂来防止皮肤光老化，提供广泛的紫外线和蓝光保护；并加入主要具有抗氧化和光保护特性的化合物，以减轻自由基形成可能产生的各种破坏作用，主要是 DNA 损伤、色素沉着和昼夜节律失调。这些物质在溶解性和光稳定性方面存在局限性，即极易降解，因此毒性风险较高。为了规避这些问题并最大限度地发挥这些成分的抗氧化潜力，纳米给药系统提供了多功能和有针对性的方法，可显著提高其生物利用率、功效和安全性。总之，本综述探讨了BL对皮肤的有益和不利影响，重点是活性成分的最新体内和体外研究，以及纳米给药系统在提高其性能方面的应用。文献研究使用的数据库有 Pubmed、Science Direct 和 Web of Science，而有关主要专利创新的研究则是通过知名专利数据库（如 Patentscope 和 Espacenet）进行的，时间跨度为 2019-2024 年。此外，本综述还探讨了化妆品成分开发过程中的可持续发展挑战，强调了这些挑战对于有效的临床转化和未来研究的重要性。
{"title":"Updated insights of active cosmetic ingredients against blue light: In vivo and in vitro evidence","authors":"Laura Ferreira , Beatriz Torres , Huma Hameed , Amélia C.F. Vieira , Sachin Kumar Singh , Kamal Dua , Francisco Veiga , Patrícia C. Pires , Priscila Gava Mazzola , Ana Cláudia Paiva-Santos","doi":"10.1016/j.jddst.2024.106306","DOIUrl":"10.1016/j.jddst.2024.106306","url":null,"abstract":"<div><div>The exponential increase in screen usage during the COVID-19 pandemic has raised concerns about higher exposure to blue light (BL) from electronic devices and raising concerns about its effects on skin health. BL significantly contributes to skin photoaging, which has aroused considerable interest among researchers due to its proximity in wavelength range to ultraviolet (UV) radiation. Owing to this high exposure and considering the high dynamism of the dermocosmetic market, formulations started to appear directed to combat skin photoaging, stimulating the interest of consumers with marketing strategies and appealing claims. These formulations have the purpose of preventing skin photoaging through the use of UV filters, providing broad UV and BL protection; and incorporation of compounds with mainly antioxidant and photoprotective properties, that allow the mitigation of various damaging effects that can occur as a result of the formation of free radicals, mainly DNA damage, hyperpigmentation, and deregulation of the circadian rhythm. These substances present limitations in solubility and photostability, i.e. high susceptibility to degradation and, consequently, a higher risk of toxicity. To circumvent these issues and maximize the antioxidant potential of these components, nanodelivery systems offer multifunctional and targeted approaches that can significantly enhance their bioavailability, efficacy, and safety. Overall, this review explores the beneficial and adverse effects of BL on the skin, with a focus on the most recent <em>in vivo</em> and <em>in vitro</em> studies of active ingredients, and the application of nanodelivery systems to enhance its performance. The databases used for literature research were Pubmed, Science Direct, and Web of Science, while research on major patent innovations was made through notorious patent databases, such as Patentscope and Espacenet, through the period of 2019–2024. Additionally, this review addresses the sustainability challenges in the development of cosmetic ingredients, highlighting their importance for effective clinical translation and future research.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1016/j.jddst.2024.106305
Sujata Saha , Rishav Kar , Kunal Sikder , Dipak Manna , Ritesh Ranjan Pal , Soumyananda Chakraborti , Ali Hossain Khan , Sourav Barman , Amit Ranjan Maity , Arnab Basu
The emergence of antibiotic resistance is one of the most challenging problems of modern times as the armory of conventional antibiotics is quickly exhausting due to the rapid rise of resistance in pathogenic microorganisms. Acinetobacter baumannii, a potential member of ESKAPE group of bacteria, has a great deal of clinical importance causing severe nosocomial infections which often become life-threatening. According to the World Health Organization (WHO), antimicrobial resistance accounts for 2–3 million deaths every year across the world. Since the pathogen has the remarkable ability to acquire or upregulate various resistant determinants in the form of secreting of β-lactamase, aminoglycoside modifying enzymes, uplifting the function of efflux pumps or altering the target sites of different antibiotics; conventional antibiotic therapy often falls short to eradicate the infection. As a possible solution, the application of alternative therapy is therefore the need of the hour. Here we emphasize potentiating the antimicrobial efficacy of pexiganan, a well-known antimicrobial peptide against Gram-negative bacteria. Conjugation with nanoparticles composed of sodium-alginate and chitosan-cholesterol has increased the antimicrobial effect of the peptide on this opportunist pathogen. The evaluation of the bactericidal analysis and minimum inhibitory concentration indicates this nanocomposite can kill the bacteria at a very low concentration (3 μg/ml). When applied to the bacterial biofilm, the key factor for producing recalcitrant infection; our synthesized complex could effectively reduce the biomass in a dose-dependent manner. Later we enfold the mechanism of killing by measuring oxidative stress, cell membrane perforation by biochemical analysis. Our analysis indicates this unique nanocomposite can bypass the need of antibiotic treatment by its pore-forming ability on bacterial membrane. The nominal in-vivo toxicity and non-specific mode of action make it an interesting candidate for future therapeutics.
{"title":"Deciphering the inhibitory mechanism of antimicrobial peptide pexiganan conjugated with sodium-alginate chitosan-cholesterol nanoparticle against the opportunistic pathogen Acinetobacter baumannii","authors":"Sujata Saha , Rishav Kar , Kunal Sikder , Dipak Manna , Ritesh Ranjan Pal , Soumyananda Chakraborti , Ali Hossain Khan , Sourav Barman , Amit Ranjan Maity , Arnab Basu","doi":"10.1016/j.jddst.2024.106305","DOIUrl":"10.1016/j.jddst.2024.106305","url":null,"abstract":"<div><div>The emergence of antibiotic resistance is one of the most challenging problems of modern times as the armory of conventional antibiotics is quickly exhausting due to the rapid rise of resistance in pathogenic microorganisms. <em>Acinetobacter baumannii</em>, a potential member of ESKAPE group of bacteria, has a great deal of clinical importance causing severe nosocomial infections which often become life-threatening. According to the World Health Organization (WHO), antimicrobial resistance accounts for 2–3 million deaths every year across the world. Since the pathogen has the remarkable ability to acquire or upregulate various resistant determinants in the form of secreting of β-lactamase, aminoglycoside modifying enzymes, uplifting the function of efflux pumps or altering the target sites of different antibiotics; conventional antibiotic therapy often falls short to eradicate the infection. As a possible solution, the application of alternative therapy is therefore the need of the hour. Here we emphasize potentiating the antimicrobial efficacy of pexiganan, a well-known antimicrobial peptide against Gram-negative bacteria. Conjugation with nanoparticles composed of sodium-alginate and chitosan-cholesterol has increased the antimicrobial effect of the peptide on this opportunist pathogen. The evaluation of the bactericidal analysis and minimum inhibitory concentration indicates this nanocomposite can kill the bacteria at a very low concentration (3 μg/ml). When applied to the bacterial biofilm, the key factor for producing recalcitrant infection; our synthesized complex could effectively reduce the biomass in a dose-dependent manner. Later we enfold the mechanism of killing by measuring oxidative stress, cell membrane perforation by biochemical analysis. Our analysis indicates this unique nanocomposite can bypass the need of antibiotic treatment by its pore-forming ability on bacterial membrane. The nominal in-vivo toxicity and non-specific mode of action make it an interesting candidate for future therapeutics.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142526194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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