Journal of Drug Delivery Science and Technology最新文献

{"title":"Multifunctional fluorescent magnetic molecularly imprinted polymer for curcumin delivery: in vivo wound healing, antibacterial activity, and in silico pharmacokinetic evaluation","authors":"Zeeshan Ali ,&nbsp;Nadeem Raza ,&nbsp;Muhammad Hayat ,&nbsp;Hafiz Muhammad Usman Abid ,&nbsp;Abdelmonaim Azzouz ,&nbsp;Bouchra Rossafi ,&nbsp;Imad Hammoudan ,&nbsp;Anis Ahmad Chaudhary ,&nbsp;Mohamed Khairy ,&nbsp;Mohamed Abdel-Megid","doi":"10.1016/j.jddst.2026.107980","DOIUrl":"10.1016/j.jddst.2026.107980","url":null,"abstract":"<div><div>Molecularly imprinted polymers (MIPs) stand out in various biomedical realms as potent and expanding nanoplatforms. This research employed a surface molecular imprinting approach<strong>,</strong> in which a polydopamine shell was polymerized around Fe₃O₄@SiO₂@FITC nanoparticles in the presence of curcumin (template)<strong>.</strong> After template removal, surface-exposed recognition sites complementary to curcumin were formed, confirming successful imprinting. The synthesized fluorescent magnetic MIPs (FMMIPs) exhibited significant drug loading capacity and responsiveness to magnetic fields, serving as efficient carriers for CUR delivery. Characterization of FMMIPs was executed through FTIR, SEM, TGA, XRD, vibrating sample magnetometry, and dynamic light scattering. The magnetization value of FMMIP was measured at 0.045 emu/g, underscoring its robust magnetic responsiveness to external fields. In-vivo studies for CUR loading and release unveiled that under tissue conditions (pH 5 and 41 °C) a maximal CUR release of 71.33 % occurred, following a sustained profile, compared to the standard physiological environment (pH 7.4 and 37 °C). In diabetic rats, treatment with FMMIP-CUR resulted in 97.55 % wound healing, surpassing the standard treatment with silver sulfadiazine. Histopathological evaluations further confirmed marked enhancements in re-epithelialization, fibroblast activity, and angiogenesis in diabetic wounds after 12 days of treatment. To explore the antibacterial potential of CUR, molecular docking and molecular dynamics (MD) simulations were conducted against <em>P. aeruginosa</em> (4KQR) and <em>S. aureus</em> (3Q8U) targets. CUR exhibited stronger binding affinities compared to ciprofloxacin, forming stable hydrogen bonds and hydrophobic interactions with key residues. The MD simulations over 100 ns revealed greater interaction stability and diversity in the 4KQR–CUR complex, while CUR's interaction with 3Q8U was more dynamic. ADMET predictions confirmed favorable pharmacokinetics and drug-likeness for both compounds, with CUR showing good oral bioavailability, moderate solubility, low toxicity, and acceptable metabolic profiles. The adsorption of CUR onto FMMIPs followed the Freundlich isotherm model (R² = 0.991) indicating heterogeneous surface binding and the presence of multiple adsorption sites with varying affinities. These results demonstrate FMMIP nanocarrier's potential as a viable option for antidiabetic drug delivery systems.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107980"},"PeriodicalIF":4.9,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposomal delivery of Ropivacaine prepared with dextran sulfate as drug-trapping polymer agent for long-acting anesthesia","authors":"Rifda Tarimi Octavia ,&nbsp;Retno Sari ,&nbsp;Ira Sari Yudaniayanti ,&nbsp;Dewi Melani Hariyadi ,&nbsp;Dwi Setyawan ,&nbsp;Gwan Young Kim ,&nbsp;Ji Yeon Heo ,&nbsp;Gwangheum Yoon ,&nbsp;Ahreum Choi ,&nbsp;Qonita Kurnia Anjani ,&nbsp;Andang Miatmoko","doi":"10.1016/j.jddst.2026.107983","DOIUrl":"10.1016/j.jddst.2026.107983","url":null,"abstract":"<div><div>The main problem invariably faced by post-operative patients is persistent pain lasting several days which affects their quality of life. Therefore, therapy providing prolonged analgesic effects is required. Rop is a group of local anesthetics offering a safer alternative to non-opioid analgesics. This study aims to develop and optimize Ropivacaine (Rop)-loaded liposome using Dextran Sulfate as a trapping polymer to enable a sustained release effect. Liposomes were composed of hydrogenated soya phosphatidylcholine and cholesterol and prepared by using the thin-film method. Optimization parameters included drug-to-lipid (D/L) ratios of 1:1 and 1:3, and incubation times of 10, 20, and 30 min. The optimized formulation (D/L ratio 1:1 and 10-min incubation) resulted in a particle size of 177.8 ± 3.82 nm, a PDI of 0.193 ± 0.01, zeta potential of −6.89 ± 4.22 mV, and an encapsulation efficiency of 72.14 ± 1.96 %. <em>In vivo</em> pain behavior was assessed in a postoperative mouse model using a hot plate test at 55 °C. The Rop liposome formulation provided effective analgesia, maintaining % Maximum Possible Effect (%MPE) values above 50 % for up to 8 h longer compared to free Rop with rapid onset within 1 h. The effect was sustained for up to 72 h without adverse reactions. The ability of the liposomal formulation to alleviate chronic pain was also demonstrated by the reduced levels of TNF-α and IL-6 in the postoperative mouse model compared to those of free Rop. In conclusion, the use of Dextran Sulfate has successfully improved the long-acting anesthesia effect of Rop-loaded liposomes.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"116 ","pages":"Article 107983"},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self nano-emulsifying drug delivery system based delivery of ketamine for enhanced therapeutic efficacy through behavioral performance in animal model","authors":"Muhammad I. Afridi ,&nbsp;Abdul Jabbar ,&nbsp;Muhammad Naseer Abbas ,&nbsp;Aziz Balouch ,&nbsp;Kausar Amir ,&nbsp;Tasmania Kanwal ,&nbsp;Huma Ikram ,&nbsp;Amjad Khan ,&nbsp;Muhammad R. Shah ,&nbsp;Sirajuddin ,&nbsp;Abdulaziz K. Al Mouslem ,&nbsp;Ghallab Alotaibi","doi":"10.1016/j.jddst.2026.107990","DOIUrl":"10.1016/j.jddst.2026.107990","url":null,"abstract":"<div><div>Ketamine drug has gained substantial interest owing to its potential for treating depression and chronic pain but its short half-life in body makes it less effective. This study was aimed at enhancing the therapeutic efficacy and safe use of anesthetic, analgesic, and antidepressant drug ketamine for its controlled drug release through self–nanoemulsifying drug delivery system (SNEDDS), following oral administration. Different excipients including Tween 80, cinnamon oil, and propylene glycol with concentration, 30 %, 53.33 %, and 16.67 %, respectively were selected for optimized SNEDDs formulation. All the developed formulations were evaluated for their droplet morphology, zeta potential, size, size distribution, robustness to dilution and thermodynamic stability. Animal studies including Skinner's box activity, open field activity, light dark box activity, plus elevated maze test, forced swim test, hot plate test and novel object recognition test, were carriedout as per standard protocol. Pharmacokinetics analysis was carried out in animal model. KT-SNEDDS demonstrated stability in pH 6.8 buffer with 1000 times dilution without precipitation or phase separation. Less than 100 nm globule size of KT-SNEDDS in solution depicted excellent flow properties. For all tests conducted on animals among all treatment groups KT-SNEDDs proved to be the best with 0.5 mg/kg dose for 12 days. <em>In-vitro</em> study demonstrated controlled and persistent release of 30–84 % drug through KT-SNEDDs as compared to burst release of 60–88 % free KT for 1–12h. <em>In-vivo</em> phamacokinetic study of KT-SNEDDS exhibited maximum time (T_max) of 4h with maximum residence time (MRT), 4.654h and maximum concentration (C_max), 339.995 μg/mL with initial dosage of 0.5 mg/kg body weight as compared to free KT with T_max of 2h, MRT, 2.637h and C_max, 253.587 μg/mL despite initial dosage of 1 mg/kg body weight. It is concluded from the study that the developed KT-SNEDDS showed better pharmaceutical, pharmacological and pharmacokinetics results, which will lead to its better therapeutic efficacy.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"116 ","pages":"Article 107990"},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disulfiram-loaded nanoemulsions for enhanced anticancer efficacy in peritoneal carcinomatosis: Design, optimization, in vitro and in vivo evaluation","authors":"Ping Wang ,&nbsp;Yue Wang ,&nbsp;Changjiang Li ,&nbsp;Zhongcheng Ke ,&nbsp;Yan Gong ,&nbsp;Boyuan Liu ,&nbsp;Meng Wang ,&nbsp;Xiangqun Jin","doi":"10.1016/j.jddst.2026.107994","DOIUrl":"10.1016/j.jddst.2026.107994","url":null,"abstract":"<div><div>Disulfiram (DSF), an FDA-approved anti-alcoholism drug, has demonstrated potent anticancer activity against peritoneal carcinomatosis. However, its therapeutic potential is severely hampered by poor aqueous solubility and instability. Nanoemulsion system provides a promising platform to overcome these challenges. Herein, this study developed and optimized a DSF-loaded nanoemulsions (DSF-NE) to enhance the therapeutic efficacy of DSF in peritoneal carcinomatosis. The optimized DSF-NE exhibited several formulation advantages, including a small particle size (∼84 nm), narrow size distribution (∼0.1), high encapsulation efficiency (&gt;90 %) and sustained-release behavior. Subsequently, cytotoxicity assays against MGC-803, SKOV-3 and Caco-2 cells revealed that DSF-NE significantly enhanced cytotoxic effects compared to DSF, with IC₅₀ values reduced by 74 %, 62 % and 56 %, respectively. Consistently, DSF-NE achieved a tumor inhibition rate 108.57 % higher than that of DSF in a murine peritoneal carcinomatosis model. The main mechanism involves enhanced cellular uptake of DSF-NE, which promotes ROS generation and subsequently triggers apoptosis. Collectively, these findings highlight DSF-NE as a nanoformulation strategy that not only improves the therapeutic performance of DSF in peritoneal carcinomatosis but also offers broader implications in the development of effective delivery systems for poorly soluble and unstable drugs.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"116 ","pages":"Article 107994"},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a novel liposome nano-formulation of quercetin with optimized cholesterol level [Lipo-Que] for intravenous administration and in vivo toxicity in mice","authors":"V. Jenitha ,&nbsp;D. David Wilson ,&nbsp;G. Monith ,&nbsp;Nampalli Babu Kokelavani ,&nbsp;Ramachandran ,&nbsp;S. Viswanathan ,&nbsp;M. Srividhya ,&nbsp;Vijay Nandana ,&nbsp;V. M. Berlin Grace","doi":"10.1016/j.jddst.2026.107984","DOIUrl":"10.1016/j.jddst.2026.107984","url":null,"abstract":"<div><div>Quercetin, a synthetic flavonoid phytochemical, has gained attention for its anti-cancer properties due to its anti-oxidant and anti-inflammatory effects, as well as its molecular actions on cancer signaling pathways. However, the therapeutic efficacy of orally administered quercetin is limited due to metabolic conversions. This study aimed to develop a novel liposomal formulation for quercetin administration via intravenous injection, utilizing thermosensitive phospholipids, cholesterol, polyethylene glycol, and folic acid. Two formulations were created (Lipo-Que 7:2:1 and Lipo-Que 5:4:1) and compared against free quercetin [Free-Que] for <em>in vitro</em> characterization, bioactivity, and biocompatibility. Both formulations were tested for anti-oxidant potency and cytotoxicity on a human lung cancer cell line and normal fetal lung fibroblast cells. The optimized dose of 25 μM quercetin was further analyzed for anti-cancer effects. Results showed that Lipo-Que 7:2:1 had significantly higher antioxidant potency and cytotoxicity (IC50 of 5.10 ± 0.19 μM) compared to Lipo-Que 5:4:1 and Free-Que. The formulations demonstrated high drug entrapment efficiency and confirmed successful encapsulation through HPLC, UV–Vis, and FTIR analyses. Additionally, Lipo-Que 7:2:1 showed enhanced quercetin release at higher temperatures and acidic pH, mimicking the cancer microenvironment. In vivo toxicity studies indicated that Lipo-Que 7:2:1 was safer than Free-Que, with no significant toxic effects observed.</div></div><div><h3>Applications</h3><div>These findings suggest that optimized liposomal quercetin formulations enhance therapeutic efficacy while maintaining stability under hyperthermic conditions (up to 45 °C). The biocompatibility results support their potential clinical application as intravenous drug candidates for lung cancer treatment.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107984"},"PeriodicalIF":4.9,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactoferrin-functionalized bioinspired grapefruit-extracellular vesicles for targeted delivery of pterostilbene in lung cancer therapy","authors":"Eman M. Nour ,&nbsp;Salma E. El-Habashy ,&nbsp;Ahmed M.R. Fath El-Bab ,&nbsp;Basant A. Bakr ,&nbsp;Riham M. El-Moslemany ,&nbsp;Amal H. El-Kamel","doi":"10.1016/j.jddst.2026.107991","DOIUrl":"10.1016/j.jddst.2026.107991","url":null,"abstract":"<div><div>Lung cancer remains the primary cause of cancer-related mortality worldwide, emphasizing the pressing need for novel phytoconstituent-based therapeutic strategies. This study investigates the lung anti-cancer potential of pterostilbene (PS), a bioactive phytochemical, incorporated into a bioinspired delivery system to improve its efficacy. PS was encapsulated within grapefruit-derived extracellular vesicles (GEV) for the development of novel (PS-GEV). Two isolation techniques were investigated: polymer precipitation and a microfluidic-assisted approach, with polymer precipitation offering superior scalability and efficiency. The resulting PS-GEV, isolated using PEG-precipitation, exhibited excellent colloidal stability, a high encapsulation efficiency exceeding 95 %, and a nanoscale particle size (30.85 ± 8.15 nm). To enhance tumor targeting and therapeutic outcomes, lactoferrin functionalization was performed (LF/PS-GEV). These modified nanocarriers showed a zeta potential of −4.03 ± 0.22 mV, particle size of 48.49 ± 13.14 nm, and a sustained release profile with 59.3 ± 4.93 % PS release over 24 h. In vitro studies on A549 lung cancer cells demonstrated enhanced cellular uptake, reduced cell viability, and inhibited cancer cell migration. The developed vesicles demonstrated high hemocompatibility. In vivo evaluation in a urethane-induced lung cancer mouse model confirmed improved therapeutic outcomes, with LF/PS-GEV treatment significantly reducing the tumor weight-to-body weight ratio (5.05 ± 0.37) compared to the untreated group. Additionally, pro-angiogenic (VEGF) and proliferative markers (cyclin D1) were downregulated, while apoptotic markers BAX (1.89 ± 0.04-fold) and caspase-3 (4.91 ± 0.09-fold) were upregulated. Histological and immunohistochemical analyses confirmed notable suppression of chemotherapy resistance-related p53 protein. Collectively, these findings highlight LF/PS-GEV as a potent, active targeting nanotherapeutic platform for effective lung cancer management through enhanced therapeutic efficacy and tumor-specific action.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107991"},"PeriodicalIF":4.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of ionizable lipid head and tail group modification on mRNA delivery efficiency","authors":"Soan Park ,&nbsp;Kanghun Lee ,&nbsp;Hyun-Jung An ,&nbsp;Enhui Lin ,&nbsp;Sugyeom Kim ,&nbsp;Hyeongseok Kim ,&nbsp;Siheon Jeong ,&nbsp;Snehagni Roy ,&nbsp;Yeon Woo Kang ,&nbsp;Jaehyuk Yang ,&nbsp;Seung-Woo Lee ,&nbsp;Gi-Ra Yi ,&nbsp;Hyomin Lee ,&nbsp;Dong-won Seo ,&nbsp;Dong Soo Hwang ,&nbsp;Joongoo Lee ,&nbsp;Jeong Wook Lee","doi":"10.1016/j.jddst.2026.107981","DOIUrl":"10.1016/j.jddst.2026.107981","url":null,"abstract":"<div><div>Ionizable lipids are crucial components of lipid nanoparticles (LNPs) that significantly impact mRNA therapeutic delivery efficiency. While SM-102 and ALC-0315 demonstrated success in Moderna and Pfizer/BioNTech COVID-19 vaccines, the relationship between their structural features and delivery performance remains unclear. We systematically modified SM-102 by incorporating ALC-0315 features: converting its asymmetric tail to a symmetric structure and replacing the 2-aminoethanol head group with ALC-0315's 4-aminobutanol group. Both modifications decreased mRNA delivery efficiency <em>in vitro</em> and <em>in vivo</em> compared to unmodified SM-102. To investigate the underlying mechanisms, we conducted independent labeling assays using DiI to track LNP internalization, and Cy3 to monitor mRNA cargo delivery. Our results revealed that while tail-modified LNPs exhibited rapid and sustained cellular uptake (DiI signal), head-modified LNPs showed relatively lower internalization. Interestingly, despite the lower uptake, the head-modified LNPs achieved significantly higher GFP expression. These findings demonstrate a clear discrepancy between internalization quantity and translation efficiency. We conclude that the absolute amount of internalized mRNA is not the sole determinant of delivery success; rather, the effective translation (functional delivery) is more critical. This study suggests that LNP structural optimization should prioritize post-internalization functional release and translation quality over simple uptake efficiency.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107981"},"PeriodicalIF":4.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of hairpin DNA-functionalized polydopamine-coated gold nanoparticles as a theranostic nanoplatform for effective delivery of daunorubicin: Binding affinity, drug release and cytotoxicity study","authors":"Jie Liu ,&nbsp;Xiangtai Wang ,&nbsp;Yushu Wu ,&nbsp;Lixia Yuan ,&nbsp;Xiusheng Zhang ,&nbsp;Xiaoyan Wu ,&nbsp;Min Liu","doi":"10.1016/j.jddst.2026.107982","DOIUrl":"10.1016/j.jddst.2026.107982","url":null,"abstract":"<div><div>Enhanced drug efflux in breast cancer cells is mediated by multiple transporters (e.g., P-glycoprotein), and the breast cancer resistance protein (BCRP) represents a key biomarker and therapeutic target among them. To address this, we constructed a pH-responsive multifunctional nanocarrier, hpDNA-PDA-AuNPs, by functionalizing polydopamine-coated gold nanoparticles with hairpin DNA (hpDNA) to deliver daunorubicin (DAU). This system integrated diagnostic imaging with three therapeutic modalities: chemotherapy, gene therapy, and photothermal therapy. It demonstrated specific targeting toward BCRP mRNA, achieving a detection limit of 0.64 nM. Spectroscopic and calorimetric analyses revealed that DAU binds more strongly to hpDNA-PDA-AuNPs than to PDA-AuNPs, which was attenuated under acidic conditions. The hpDNA-PDA-AuNPs + DAU system exhibited pH-responsive drug release, enhanced by NIR irradiation and BCRP mRNA. The combined treatment demonstrated potent synergistic antitumor activity with nuclear accumulation of DAU, offering a promising platform to overcome multidrug resistance via programmable drug release, gene silencing, and photothermal ablation.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"116 ","pages":"Article 107982"},"PeriodicalIF":4.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual release of miRNA-424-3p and doxorubicin using layer-by-layer stimuli-responsive dendritic mesoporous nanoparticles","authors":"Oznur Akbal Vural ,&nbsp;Tayfun Vural ,&nbsp;Vicente Martí-Centelles ,&nbsp;Estela Climent ,&nbsp;Alba García-Fernández ,&nbsp;Ramón Martínez-Máñez","doi":"10.1016/j.jddst.2026.107986","DOIUrl":"10.1016/j.jddst.2026.107986","url":null,"abstract":"<div><div>The prevalence of cancer is on the rise on a global scale, and lung cancer represents one of the most common types of cancer worldwide that leads to high cancer-related deaths. Over the past decade, nanotechnology has made tremendous progress in cancer treatment. Moreover, combination therapy, including chemotherapy and gene therapy, has turned out to be a promising strategy that could enhance therapeutic efficacy in the treatment of lung adenocarcinoma. The tumor-suppressive miRNA-424-3p has demonstrated to significantly downregulate galectin-3 (Gal3) which is an anti-apoptotic protein and highly expressed in A549 cells. This has been shown to promote apoptosis, decrease proliferation, and increase sensitivity to the chemotherapeutic drug doxorubicin (Dox). However, miRNA-424-3p-based combination therapies with Dox have not yet been reported. Since dendritic mesoporous silica nanoparticles (DMSNs) have the potential to act as carriers for nucleic acids and drugs simultaneously, in this study we have successfully introduced for the first time anti-Gal3 functionalized DMSNs for the co-delivery of miRNA-424-3p and Dox protected with a layer-by-layer polyethyleneimine and poly-L-lysine coating. Whereas there is no or minimal Dox and miRNA release under neutral pH from the nanoparticles, conversely, a significant delivery of Dox and miRNA is seen in lysosomal extract medium. The effects of the co-delivery system, i.e. miRNA-424-3p and Dox, on A549 cells is evaluated by analyzing cell proliferation, cellular uptake, cell apoptosis assay, and the expression of related proteins and genes. The results demonstrate that miRNA-424-3p effectively transfected into A549 lung cancer cells, dramatically reducing the expression of Gal3 at the protein level. Forward transfection of the cell line with the DMSN loaded with miRNA-424-3p and Dox causes both a decrease in cell viability and an increase in apoptosis induction. Our data shows that the designed DMSN has a good performance as a combined drug and gene delivery system for cancer therapy.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107986"},"PeriodicalIF":4.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae-derived vesicles as potential carriers for therapeutic biomacromolecules","authors":"Mária Klacsová ,&nbsp;Krisztina Sebők-Nagy ,&nbsp;Tibor Páli ,&nbsp;Marcela Chovancová ,&nbsp;László Almásy ,&nbsp;Daniela Uhríková","doi":"10.1016/j.jddst.2026.107985","DOIUrl":"10.1016/j.jddst.2026.107985","url":null,"abstract":"<div><div>Microalgae-derived vesicles (AGVs) were investigated as potential carriers for therapeutic biomacromolecules. AGVs were prepared by rehydration of a lyophilisate of proteolipid ghost membranes from <em>Dunaliella tertiolecta</em> and subsequent extrusion or sonication. Based on our results, extrusion is suggested to be a more suitable method because of the low loss of the lipid and protein content. Applying small-angle neutron scattering and dynamic and electrophoretic light scattering, we found that the formed AGVs are spherical, unilamellar, with moderate polydispersity, and a negative surface charge of −27 mV. EPR findings show that they retain the membrane dynamics, and possibly the degree of lipid chain saturation, of the ‘mother’ algal cells. The drug-binding efficiency of the reconstructed AGVs was tested for two model biomacromolecules, DNA and BSA, using an ethidium bromide exclusion assay and tryptophane autofluorescence, respectively. The electrostatic interaction between AGVs and DNA polyanion was mediated by Ca²⁺ ions, while for the interaction of BSA with AGVs hydrophobic interactions are assumed to play a dominant role. For both macromolecules tested, 40 % binding of the total drug amount was achieved by passive incubation, which indicates that reconstructed AGVs might expand the repertoire of potential delivery systems for therapeutic biomacromolecules.</div></div>","PeriodicalId":15600,"journal":{"name":"Journal of Drug Delivery Science and Technology","volume":"117 ","pages":"Article 107985"},"PeriodicalIF":4.9,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}