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Development of Gold Nanoparticle (AuNP)-based Colorimetric Aptasensor for Penicillin G Detection 纳米金(AuNP)基青霉素G比色传感器的研制
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.13
Darmawati Darmawati, A. Z. Mustopa, B. Budiarto, S. Rahmawati, F. Izzati, R. Harmoko, E. Saepudin, A. Mahsunah
Antibiotics are chemical or biological substances that have the ability to kill pathogens selectively. Currently, high-performance liquid chromatography (HPLC) is used routinely in the detection of antibiotics. However, the cost of analysis and running time are bottlenecks for HPLC to be used for routine tests to detect antibiotics. Alternative methods need to be developed to overcome this issue. In this study, the development of a penicillin G specific biosensor by using a DNA aptamer and gold nanoparticles (AuNPs) was done. Optimal aptasensor conditions were achieved with the concentrations of NaCl and aptamer at 0.25 M and 2 μM, respectively. An aptasensor of this type showed LOD for penicillin G at 3 mg/L and was able to detect penicillin G in the range of 3 to 27 mg/L. The established aptasensor showed specific sensitivity toward penicillin G after testing with several antibiotics, i.e., ampicillin, kanamycin, chloramphenicol, and erythromycin. The aptasensor could detect the presence of penicillin G from culture medium of wild-type, ultraviolet irradiation mutant, gamma irradiation mutant, and ultraviolet irradiation and gamma irradiation mutant strains of P. chrysogenum, at detection concentrations of 9.75 ± 0.004; 25.25 ± 0.005; 37.5 ± 0.005; and 45 ± 0.004 mg/L, respectively.
抗生素是具有选择性杀死病原体能力的化学或生物物质。目前,高效液相色谱法是抗生素检测的常用方法。然而,分析成本和运行时间是制约高效液相色谱用于常规检测抗生素的瓶颈。需要开发替代方法来克服这个问题。本研究利用DNA适体和金纳米粒子(AuNPs)制备青霉素G特异性生物传感器。适体浓度为0.25 M和2 μM时,适体传感器的最佳条件为NaCl和适体。该型配体传感器对青霉素G的检出限为3 mg/L,对青霉素G的检出范围为3 ~ 27 mg/L。经氨苄西林、卡那霉素、氯霉素、红霉素等多种抗生素检测,所建立的适体传感器对青霉素G具有特异性敏感性。该传感器能检测出野生型、紫外辐照突变株、γ辐照突变株、紫外辐照和γ辐照突变株培养基中青霉素G的存在,检测浓度为9.75±0.004;25.25±0.005;37.5±0.005;分别为45±0.004 mg/L。
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引用次数: 0
Impact of Al on ZnO Electron Transport Layer in Perovskite Solar Cells 铝对钙钛矿太阳能电池中ZnO电子传递层的影响
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.9
Nur Syafiqah Nadiah Mohd Alias, F. Arith, A. N. Mustafa, MohdMuzafar Ismail, Nur Fatihah Azmi, M. S. Saidon
Perovskite solar cells have shown remarkable performance and improvements in terms of solar cell efficiency. The ETL material is one of the important components in perovskite solar cells in conducting electrons to produce current. Here, ZnO was used as ETL material in a perovskite solar cell using the SCAPS 1D simulation software. The ZnO ETL showed poor cell efficiency due to its reaction with the perovskite material. A small amount of Al doped into ZnO was introduced to enhance the physiochemical properties of the ZnO against perovskite materials. Al concentrations were varied between 1 and 4 mol% to observe the effect on cell efficiency. Compared with a conventional ZnO ETL solar cell with 0 mol% Al perovskite, the Al-doped based solar cell showed better performance. Meanwhile, perovskite solar cells with 1 mol% Al-doping and appropriate layer thickness showed the best cell performance in improving the charge transport mechanism, resulting in increased cell efficiency. Thus, the parameters studied can be a guide in the fabrication process.
钙钛矿太阳能电池在太阳能电池效率方面表现出了显著的性能和改进。ETL材料是钙钛矿太阳能电池中导电电子产生电流的重要组成部分之一。在这里,使用SCAPS 1D模拟软件将ZnO用作钙钛矿太阳能电池的ETL材料。ZnO ETL由于与钙钛矿材料发生反应,电池效率较低。将少量Al掺杂到ZnO中,提高了ZnO对钙钛矿材料的理化性能。Al浓度在1 ~ 4 mol%之间变化,观察对细胞效率的影响。与含有0 mol% Al钙钛矿的传统ZnO ETL太阳能电池相比,Al掺杂基太阳能电池表现出更好的性能。同时,钙钛矿太阳能电池中掺杂1mol % al和适当层厚的钙钛矿太阳能电池在改善电荷输运机制方面表现出最好的电池性能,从而提高了电池效率。因此,所研究的参数可以指导加工过程。
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引用次数: 3
The Determination of Algae Group as Bioindicator of Water Quality Change Affected by Mercury Release from Artisanal Small-Scale Gold Mining (ASGM) 小型手工金矿汞排放对水质变化影响的藻类群测定
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.14
H. D. Ariesyady, Indah Yusliga Sari Purba
Artisanal small scale gold mining (ASGM) practices typically use mercury for amalgamation. Near water environments this can degrade water quality and aquatic biota, including algae. Changes in algal communities can reflect water environment disturbance. The aim of this study was to determine if algae can be used as bioindicator of river water quality impacted by ASGM activities. The research was conducted from July to October 2018 at thirty sampling sites along rivers near ASGM areas in several regencies of Indonesia. Composite samples of water and sediment were collected. A plankton net and brushing methods were used to collect planktonic and benthic algae, respectively. The physicochemical parameters of the water and the sediment as well as the dominant algae genera were analyzed statistically with principal component analysis. The results showed that the total mercury concentration in the water ranged from <0.04 to 20 µg.L-1, while in the sediment the maximum value was 13,500 µg.kg-1. The total mercury content in the sediment was negatively correlated with the dominant benthic Navicula at a significance level of p < 0.05. This means that a low density of benthic Navicula can be proposed as a bioindicator of water quality, indicating the increase of mercury pollution in sediment.
手工小规模金矿开采(ASGM)通常使用汞进行合并。在近水环境中,这会降低水质和水生生物群,包括藻类。藻类群落的变化可以反映水环境的扰动。本研究的目的是确定藻类是否可以用作受ASGM活动影响的河流水质的生物指标。这项研究于2018年7月至10月在印度尼西亚几个县ASGM地区附近河流沿岸的30个采样点进行。采集了水和沉积物的复合样品。浮游生物网和刷洗法分别用于收集浮游藻类和底栖藻类。采用主成分分析法对水体和沉积物的理化参数以及优势藻类属进行了统计分析。结果表明,水中的总汞浓度在<0.04至20µg.L-1之间,而沉积物中的最大值为13500µg.kg-1。沉积物中的总汞含量与优势底栖Navicula呈负相关,显著性水平为p<0.05。这意味着可以将低密度的海底Navicula作为水质的生物指标,表明沉积物中汞污染的增加。
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引用次数: 0
Comparative Analysis of Compression Molded Products of Recycled Waste Poly(Vinyl Chloride) and Virgin Poly(Vinyl Chloride) Fill Material 回收废旧聚氯乙烯与原聚氯乙烯填充材料压缩成型制品的对比分析
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.12
S. Sultana, Md. Khabir Uddin Sarker, Z. Islam, M. S. Islam
Waste poly(vinyl chloride) fill material from the cooling tower of a power plant was used for mechanical recycling. Mechanical recycling is the processing of plastic waste without changing the original chemical structure of the plastic. The waste rigid poly(vinyl chloride) fill material was cleaned, grinded and compression molded at optimized conditions of time, temperature and pressure using a compression molding machine. Virgin poly(vinyl chloride) was purchased and compression molded by the same compression molding machine. The compression molded sheets of waste poly(vinyl chloride) and virgin poly(vinyl chloride) were characterized by attenuated total reflection Fourier transform infrared analyses, tensile properties analyses, and thermal properties analyses. The results revealed that waste rigid poly(vinyl chloride) fill material is mechanically recyclable into new products such as pipes, profiles, furniture and other related products.
发电厂冷却塔的废弃聚氯乙烯填料被用于机械回收。机械回收是指在不改变塑料原有化学结构的情况下处理塑料垃圾。使用压缩成型机在优化的时间、温度和压力条件下对废弃的硬质聚氯乙烯填充材料进行清洁、研磨和压缩成型。购买原始聚氯乙烯,并用同一台压缩成型机进行压缩成型。通过衰减全反射傅立叶变换红外分析、拉伸性能分析和热性能分析,对废弃聚氯乙烯和原始聚氯乙烯压制成型片材进行了表征。结果表明,废弃的硬质聚氯乙烯填充材料可以机械回收,制成新产品,如管道、型材、家具和其他相关产品。
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引用次数: 1
Strength Enhancement, Ductility, and Confinement Effectiveness Index of Fly Ash-based Geopolymer Concrete Square Columns 粉煤灰基地聚合物混凝土方柱的强度增强、延性及约束效能指标
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.11
Herwani Herwani, I. Imran, B. Budiono, Ediansjah Zulkifli
Geopolymer concrete is an environmentally friendly construction material that has the potential to be applied in building structures. It is important to understand the structural behavior of geopolymer concrete. This paper presents an experimental investigation into the performance of structural elements of geopolymer concrete under concentric axial loads. The specimens were twelve square columns with a size of 170 x 170 mm and a height of 480 mm. The study variables were the tie spacing and the compressive strength of unconfined geopolymer concrete (f’c). The test results showed that the increase in f'cc was not as significant as the increase in unconfined concrete compressive strength (f'c). The value of strength enhancement (K) tended to decrease. The column ductility (m) and confinement effectiveness index (Ic) had optimum values. The effect of increasing the tie spacing (s) decreased the K, Ic, and m values of the column. The proposed f’cc formulation for geopolymer concrete is compatible.
地质聚合物混凝土是一种环境友好的建筑材料,具有应用于建筑结构的潜力。了解地质聚合物混凝土的结构行为非常重要。本文对地质聚合物混凝土结构构件在同心轴向荷载作用下的性能进行了试验研究。试样为12根尺寸为170 x 170 mm、高度为480 mm的方柱。研究变量为无侧限地质聚合物混凝土的连接间距和抗压强度。试验结果表明,f’cc的增加不如无侧限混凝土抗压强度的增加显著。强度增强值(K)呈下降趋势。柱的延性(m)和约束有效性指数(Ic)具有最佳值。增加连接间距的影响降低了柱的K、Ic和m值。所提出的用于地质聚合物混凝土的f'cc配方是相容的。
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引用次数: 1
Microwave-assisted Synthesis of Functionalized Multiwalled Carbon Nanotube–Titanium Dioxide Hybrid Structure and Photodegradation 微波辅助合成功能化多壁碳纳米管-二氧化钛杂化结构及其光降解
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.7
Y. Irmawati, Shofia Manzalini, B. Sugeng, S. Sudirman, Harayasu Asahara, R. Yudianti
Decoration of a functionalized multiwalled carbon nanotube (f-MWCNT) surface with titanium dioxide (TiO2) was designed to improve its photocatalytic degradation performance. Structural decoration was achieved by microwave heating at various mass ratios (1:2; 1:4; 1:8; 1:16 wt.%) of titanium (IV) isopropoxide as precursor. The hybrid structure of TiO2/f-MWCNT was characterized by scanning electron microscope and transmission electron microscope (TEM). The crystallite form of the TiO2 nanoparticles was further studied by X-ray diffraction (XRD) and HR–TEM. We report the conformation of high-density TiO2 coated on an f-MWCNT surface at a mass ratio of 1:16 wt.%. XRD analysis revealed a structural transformation from mixture phase (anatase–brookite) at mass ratios of 1:2 and 1:4 wt.% to fully anatase phase for mass ratios of 1:8 and 1:16 wt.%. The transformation was also confirmed by selected area electron diffraction (SAED) and HR–TEM analysis. Our results showed that anatase phase plays a significant role in photodegradation activity.
设计了用二氧化钛(TiO2)修饰功能化多壁碳纳米管(f-MWCNT)表面,以提高其光催化降解性能。通过在不同质量比(1:2;1:4;1:8;1:16wt%)的异丙氧基钛(IV)作为前体的微波加热来实现结构装饰。用扫描电子显微镜和透射电子显微镜对TiO2/f-MWCNT的杂化结构进行了表征。通过X射线衍射(XRD)和HR–TEM进一步研究了TiO2纳米颗粒的微晶形式。我们报道了以1:16 wt.%的质量比涂覆在f-MWCNT表面上的高密度TiO2的构象。XRD分析显示,从质量比为1:2和1:4 wt.%时的混合相(锐钛矿-板钛矿)到质量比为1:8和1:16 wt.%时的完全锐钛矿相的结构转变。选区电子衍射(SAED)和HR–TEM分析也证实了这种转变。结果表明,锐钛矿相在光降解活性中起着重要作用。
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引用次数: 1
Modeling Salinity Propagation in Cikarang Bekasi Laut Channel, Bekasi Regency, West Java Province, Indonesia 印度尼西亚西爪哇省贝卡西县Cikarang Bekasi Laut海峡盐度传播模型
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.10
Harman Ajiwibowo, M. B. Pratama
An inland waterway will be constructed in an existing channel in West Java, Indonesia. The change of salinity in a river due to capital dredging is investigated using a finite element modeling system. The location of the research is the Cikarang Bekasi Laut (CBL) channel, Bekasi Regency, West Java, Indonesia. The aim of this study is to simulate salinity propagation in the CBL before and after capital dredging. Salinity modeling is carried out using the Surface-water Modeling System (SMS). Field surveys of the topography, bathymetry, currents and water level are conducted for the model. The model results are validated with the current and water level field measurements. The field and model results agree well. The current and salinity results show that the trends are correlated with river discharges. After the capital dredging, the salinity in the CBL increases. Mitigation is carried out by constructing a check dam, which can reduce the average salinity in the upstream region by up to 0.05 ppt during the wet season and up to 0.12 ppt during the dry season.
将在印度尼西亚西爪哇的一条现有航道上修建一条内河航道。利用有限元建模系统研究了疏浚引起的河流盐度变化。研究地点位于印度尼西亚西爪哇省贝卡西县的Cikarang Bekasi Laut(CBL)通道。本研究的目的是模拟基建疏浚前后CBL中的盐度传播。盐度建模是使用地表水建模系统(SMS)进行的。对该模型进行了地形、水深、洋流和水位的实地调查。模型结果通过电流场和水位场测量进行了验证。现场和模型结果一致。水流和盐度结果表明,趋势与河流流量相关。资本疏浚后,CBL中的盐度增加。缓解措施是通过建造一座拦水坝来实现的,这可以在雨季将上游地区的平均盐度降低0.05 ppt,在旱季将其降低0.12 ppt。
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引用次数: 0
Simulation Model of Enhancing Performance of TCP/AQM Networks by Using Matlab 基于Matlab的TCP/AQM网络性能增强仿真模型
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.4
G. Aziz, Manal Hadi Jaber, Mohammed Qasim Sulttan, S. W. Shneen
Internet networks are becoming more crowded every day due to the rapid development of modern life, which causes an increase in the demand for data circulating on the Internet. This creates several problems, such as buffer overflow of intermediate routers, and packet loss and time delay in packet delivery. The solution to these problems is to use a TCP/AQM system. The simulation results showed that there were differences in performance between the different controllers used. The proposed methods were simulated along with the required conditions in nonlinear systems to determine the best performance. It was found that the use of optimization Department of Electro-mechanical Engineering, University of Technology - Iraq tools (GA, FL) with a controller could achieve the best performance. The simulation results demonstrated the ability of the proposed methods to control the behavior of the system. The controller systems were simulated using Matlab/Simulink. The simulation results showed that the performance was better with the use of GA-PIDC compared to both FL-PIDC and PIDC in terms of stability time, height, and overrun ratio for a network with a variable queue that was targeted for comparison. The results were: the bypass ratio was 0, 3.3 and 21.8 the settling time was 0.002, 0.055, and 0.135; and the rise time was 0.001, 0.004 and 0.008 for GA-PIDC, FL-PIDC and PIDC, respectively. These results made it possible to compare the three control techniques.
由于现代生活的快速发展,互联网每天都变得越来越拥挤,这导致对互联网上流通的数据的需求增加。这会产生一些问题,例如中间路由器的缓冲区溢出,以及数据包传递中的数据包丢失和时间延迟。解决这些问题的方法是使用TCP/AQM系统。仿真结果表明,所使用的不同控制器在性能上存在差异。对所提出的方法以及非线性系统中所需的条件进行了模拟,以确定最佳性能。研究发现,使用伊拉克理工大学机电工程系优化工具(GA,FL)与控制器可以获得最佳性能。仿真结果证明了所提出的方法控制系统行为的能力。利用Matlab/Simulink对控制器系统进行了仿真。仿真结果表明,对于具有可变队列的网络,与FL-PIDC和PIDC相比,GA-PIDC的性能在稳定时间、高度和溢出率方面更好。结果表明:分流比分别为0、3.3和21.8,沉降时间分别为0.002、0.055和0.135;GA-PIDC、FL-PIDC和PIDC的上升时间分别为0.001、0.004和0.008。这些结果使得比较这三种控制技术成为可能。
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引用次数: 1
Geothermal System in Parang Wedang, Yogyakarta, Indonesia 印尼日惹Parang Wedang的地热系统
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.6
M. Iqbal, B. R. Juliarka
Geothermal manifestations in Parangtritis, Indonesia, known as Parang Wedang Hot Spring, indicate a geothermal system in the subsurface. This circumstance motivated our research to model the Parang Wedang geothermal system in order to determine its subsurface conditions. Geological mapping, the geophysical method, and geochemical analysis were integrated to produce a conceptual model of the Parang Wedang geothermal system. The study area consists of structural hills, karst hills, and eolian plains with andesite breccias, limestone, andesite, and sand deposits as lithological variations. The results of magnetic modeling indicate that the research area is associated with the presence of an andesite intrusion and shows a NE-SW trending geological structure that is thought to be a path for hydrothermal fluid to the surface. Geochemical analysis was performed at two hot springs with temperatures of 47 °C and 49 °C. Geothermometer calculation showed that the geothermal reservoir in Parang Wedang has a temperature of 100 to 120 °C, a depth of about 180 to 285 m, and can be classified as a low enthalpy geothermal system.
印尼Parangtritis的地热表现被称为Parang Wedang温泉,表明地下有地热系统。这种情况促使我们对Parang Wedang地热系统进行建模,以确定其地下条件。地质测绘、地球物理方法和地球化学分析相结合,形成了巴朗-韦当地热系统的概念模型。研究区由构造丘陵、岩溶丘陵和风成平原组成,岩性变化包括安山岩角砾岩、石灰岩、安山岩和砂矿床。磁性建模结果表明,该研究区域与安山岩侵入体的存在有关,并显示出NE-SW走向的地质结构,这被认为是热液流到达地表的路径。对两个温度分别为47°C和49°C的温泉进行了地球化学分析。地质温度计计算表明,Parang Wedang的地热储层温度为100至120°C,深度约为180至285米,可归类为低焓地热系统。
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引用次数: 0
Design of Split-Hopkinson Pressure Bar Specimen Fixture to Accommodate Punch and Double-Notch Shear Testing 适应冲孔和双缺口剪切试验的分离式霍普金森压杆试样夹具的设计
IF 0.9 Q3 Engineering Pub Date : 2022-07-18 DOI: 10.5614/j.eng.technol.sci.2022.54.4.8
Arya Dipajaya, Kemal I. Ahmad, M. A. Kariem
This study focused on the design of a specimen fixture which can be installed on the end of a conventional transmission bar so that shear testing (punch and double-notch) can be conducted with any conventional split-Hopkinson apparatus. The research was conducted by using the finite element method in Abaqus/CAE with 6061-T651 Aluminum as the specimen material. The research successfully determined the effect of the fixture’s geometry and dimensions on the split-Hopkinson shear bar testing results. The optimum double-notch fixture provides great accuracy, having only a shear stress value difference of 1.49% with the original setup, while attaining force equilibrium after only 70 μs. The punch fixture, however, could only reach force equilibrium after 100 μs, thus providing too few observable data. Future work on the punch fixture is needed.
本研究的重点是设计一种试样夹具,该夹具可以安装在传统传动杆的末端,以便可以使用任何传统的劈裂霍普金森装置进行剪切测试(冲孔和双缺口)。以6061-T651铝合金为试样材料,采用Abaqus/CAE软件进行有限元分析。研究成功地确定了夹具的几何形状和尺寸对劈裂-霍普金森剪切杆试验结果的影响。优化后的双缺口夹具精度高,剪切应力值与原夹具的差值仅为1.49%,且只需70 μs即可达到力平衡。然而,冲床夹具只能在100 μs后达到力平衡,因此提供的观测数据太少。需要对冲床夹具进行未来的工作。
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引用次数: 2
期刊
Journal of Engineering and Technological Sciences
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