The Ferronikel smelter in Drenas is one of the main industrial areas in the Kosovo and pollution by heavy metals causes serious threat for all living organisms on this area. The objective of this study was to determine the concentration of some heavy metals (Fe, Cu, Mn, Cr, Cd, Ni and Pb) in agricultural soils and in maize plants, and their potential toxic effects on this plant through some sensitive biochemical and molecular markers. Maize seedlings growth in nine soil samples from different locations of this area. The highest concentrations of heavy metals in soils and maize leaves were conducted close to the Ferronikel smelter, and in some locations, the nickel and chromium concertation in soils exceeded 800 mg kg-1. A significant effects of heavy metals induced toxicity resulted in the, build-up aminolevulinic acid and reduced activity of δ-aminolevulinic acid dehydratase, and chlorophyll content in the maize leaves. In general, maize seedlings growth in polluted locations showed an increase in nuclear DNA content and in G2M phase. We concluded that locations close to the smelter are affected by soil heavy metals pollution and these biochemical and molecular analysis would be a powerful ecotoxicological tool in biomonitoring of heavy metal pollution.
{"title":"Stress induced by soil contamination with heavy metals and their effects on some biomarkers and DNA damage in maize plants at the vicinity of Ferronikel smelter in Drenas, Kosovo.","authors":"Liridon Buqaj, Bekim Gashi, Muhamet Zogaj, Ramë Vataj, Valbona Sota, Metin Tuna","doi":"10.1080/03601234.2023.2253114","DOIUrl":"10.1080/03601234.2023.2253114","url":null,"abstract":"<p><p>The Ferronikel smelter in Drenas is one of the main industrial areas in the Kosovo and pollution by heavy metals causes serious threat for all living organisms on this area. The objective of this study was to determine the concentration of some heavy metals (Fe, Cu, Mn, Cr, Cd, Ni and Pb) in agricultural soils and in maize plants, and their potential toxic effects on this plant through some sensitive biochemical and molecular markers. Maize seedlings growth in nine soil samples from different locations of this area. The highest concentrations of heavy metals in soils and maize leaves were conducted close to the Ferronikel smelter, and in some locations, the nickel and chromium concertation in soils exceeded 800 mg kg<sup>-1</sup>. A significant effects of heavy metals induced toxicity resulted in the, build-up aminolevulinic acid and reduced activity of δ-aminolevulinic acid dehydratase, and chlorophyll content in the maize leaves. In general, maize seedlings growth in polluted locations showed an increase in nuclear DNA content and in G2M phase. We concluded that locations close to the smelter are affected by soil heavy metals pollution and these biochemical and molecular analysis would be a powerful ecotoxicological tool in biomonitoring of heavy metal pollution.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"617-627"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10163323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-10-12DOI: 10.1080/03601234.2023.2264743
Arkadeb Mukhopadhyay, Shubham Chaudhary, Jyoti Antil, Vishal S Somvanshi, Suresh M Nebapure, Neeraj Patanjali, Anirban Dutta, Subhash Babu, Chellapilla Bharadwaj, Susama Sudhishri, Man Singh, Tirthankar Banerjee, Anil Kumar, Anupama Singh
The application of biocontrol agents in farm operations for pest control programs is gaining priority and preference globally. Effective delivery, infectivity of the biocontrol agents, and quality shelf-life products containing these bioagents are vital parameters responsible for the success of biopesticides under field conditions. In the present study, moisture-retaining bio-insecticidal dustable powder formulation (SaP) of Steinernema abbasi (Sa) infective juveniles (IJs) was developed and assessed for its shelf life, physicochemical profile, and bio-efficacy against subterranean termite under field conditions. Formulation exhibited free-flowing character, with pH of 6.50-7.50, and apparent density in the range 0.50-0.70 g cm-3. The bioefficacy study for two rabi seasons (2020-2021, and 2021-2022) in wheat and chickpea grown in an experimental farm heavily infested with subterranean termites (Odontotermes obesus) revealed a significant reduction in plant damage due to pest attack in formulation-treated plots, monitored in terms of relative number of infested tillers in wheat and infested plants in chickpea fields. The reduced damage to the crop caused by termite was reflected in the relative differences in the growth and yield attributes as well. The study establishes the potential of the developed product as a biopesticide suitable for organic farming and integrated pest management operations.
生物防治剂在农场病虫害防治项目中的应用正在全球范围内获得优先权和偏好。生物防治剂的有效递送、传染性和含有这些生物制剂的保质期产品是生物农药在田间条件下成功的重要参数。在本研究中,开发了一种具有感染力的Steinerma abbasi(Sa)幼虫(IJs)的保湿生物杀虫可尘粉末制剂(SaP),并在田间条件下对其保质期、理化特性和对地下白蚁的生物功效进行了评估。配方表现出自由流动特性,pH为6.50-7.50,表观密度在0.50-0.70范围内 g cm-3。在深受地下白蚁侵扰的实验农场(Odontotermes obesus)种植的小麦和鹰嘴豆的两个拉比季节(2020-2021年和2021-2022年)的生物有效性研究表明,在配方处理的地块中,由于害虫攻击而造成的植物损害显著减少,并根据小麦受感染分蘖和鹰嘴豆田受感染植物的相对数量进行监测。白蚁对作物造成的损害减少也反映在生长和产量属性的相对差异上。该研究确定了开发产品作为一种生物杀虫剂的潜力,适用于有机农业和病虫害综合治理作业。
{"title":"Novel moisture retaining dustable powder containing <i>Steinernema abbasi</i> effectively controls damage of subterranean termite in wheat and chickpea.","authors":"Arkadeb Mukhopadhyay, Shubham Chaudhary, Jyoti Antil, Vishal S Somvanshi, Suresh M Nebapure, Neeraj Patanjali, Anirban Dutta, Subhash Babu, Chellapilla Bharadwaj, Susama Sudhishri, Man Singh, Tirthankar Banerjee, Anil Kumar, Anupama Singh","doi":"10.1080/03601234.2023.2264743","DOIUrl":"10.1080/03601234.2023.2264743","url":null,"abstract":"<p><p>The application of biocontrol agents in farm operations for pest control programs is gaining priority and preference globally. Effective delivery, infectivity of the biocontrol agents, and quality shelf-life products containing these bioagents are vital parameters responsible for the success of biopesticides under field conditions. In the present study, moisture-retaining bio-insecticidal dustable powder formulation (SaP) of <i>Steinernema abbasi</i> (<i>Sa</i>) infective juveniles (IJs) was developed and assessed for its shelf life, physicochemical profile, and bio-efficacy against subterranean termite under field conditions. Formulation exhibited free-flowing character, with pH of 6.50-7.50, and apparent density in the range 0.50-0.70 g cm<sup>-3</sup>. The bioefficacy study for two rabi seasons (2020-2021, and 2021-2022) in wheat and chickpea grown in an experimental farm heavily infested with subterranean termites (<i>Odontotermes obesus</i>) revealed a significant reduction in plant damage due to pest attack in formulation-treated plots, monitored in terms of relative number of infested tillers in wheat and infested plants in chickpea fields. The reduced damage to the crop caused by termite was reflected in the relative differences in the growth and yield attributes as well. The study establishes the potential of the developed product as a biopesticide suitable for organic farming and integrated pest management operations.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":" ","pages":"679-688"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-10-05DOI: 10.1080/03601234.2023.2262348
Elham Pournamdari, Leila Niknam
In this study, a resonance Rayleigh scattering technique-based sensing method for detecting Bentazone residual in water samples has been developed. This technique was carried out using chitosan-capped gold nanoparticles with a spectrofluorimetric method. Experimental results revealed that the developed method could allow the detection of Bentazone residual as low as a concentration of 0.02 ng mL-1 within 50-sec time. Overall results confirmed the very low detection limit for measuring the Bentazone. The chitosan-capped gold nanoparticles as an excellent sensor were applied to measure and analyze Bentazone in water samples.
本研究开发了一种基于共振瑞利散射技术的检测水样中苯达松残留量的传感方法。该技术是用荧光光谱法使用壳聚糖封端的金纳米粒子进行的。实验结果表明,所开发的方法可以检测浓度低至0.02的Bentazone残留 ng mL-1。总体结果证实了用于测量Bentazone的检测限非常低。将壳聚糖包覆的金纳米粒子作为一种优良的传感器应用于水样中苯达松的测定和分析。
{"title":"Resonance Rayleigh scattering technique-using chitosan-capped gold nanoparticles, approaches spectrofluorimetric method for determination of Bentazone residual in water samples.","authors":"Elham Pournamdari, Leila Niknam","doi":"10.1080/03601234.2023.2262348","DOIUrl":"https://doi.org/10.1080/03601234.2023.2262348","url":null,"abstract":"<p><p>In this study, a resonance Rayleigh scattering technique-based sensing method for detecting Bentazone residual in water samples has been developed. This technique was carried out using chitosan-capped gold nanoparticles with a spectrofluorimetric method. Experimental results revealed that the developed method could allow the detection of Bentazone residual as low as a concentration of 0.02 ng mL<sup>-1</sup> within 50-sec time. Overall results confirmed the very low detection limit for measuring the Bentazone. The chitosan-capped gold nanoparticles as an excellent sensor were applied to measure and analyze Bentazone in water samples.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 10","pages":"628-636"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-10-05DOI: 10.1080/03601234.2023.2263191
Marwa Tamim A Abdel-Wareth, Rania M Abd El-Hamid
Contamination of water and sediment with pyrethroids and triazoles residues can affect fungal diversity, and hence the aquatic system functioning. The aim of the present study is to investigate the effect of water and sediment contamination with pyrethroids and triazoles on fungal diversity. Water and sediment samples were seasonally collected along 2019 from water bodies representing Gharbeya and Qualubeya governorates. Concentrations of pyrethroids and triazoles were determined, and fungal species in water and sediment samples were molecularly identified. The results indicated that temperature and pH varied according to the season. Permethrin, deltamethrin, lambda-cyhalothrin and esfenvalerate showed the highest pyrethroids concentrations, whereas tetraconazole, tebuconazole and difenconazole were of the highest triazoles concentrations. Aspergillus niger was one of the most frequent species, in addition to Trichoderma capillare, Fusarium oxysporum, Penicillium commune and Penicillium polonicum. Principal component analysis indicated a positive correlation between temperature and different Aspergillus spp., and between pH and each of pyrethroids and triazoles. Moreover, a negative correlation was observed between triazoles and Trichoderma asperellum, Penicillium griseofulvum and Aspergillus fumigatus. In conclusion, contamination of water with pesticides affected fungal diversity. This disturbance in fungal assemblages might result in a reduction of some key organisms, or an increase and emergence of new pathogens.
{"title":"Residues of pyrethroids and triazoles pesticides in water and sediment of certain Egyptian watercourses: assessing their influence on fungal diversity.","authors":"Marwa Tamim A Abdel-Wareth, Rania M Abd El-Hamid","doi":"10.1080/03601234.2023.2263191","DOIUrl":"https://doi.org/10.1080/03601234.2023.2263191","url":null,"abstract":"<p><p>Contamination of water and sediment with pyrethroids and triazoles residues can affect fungal diversity, and hence the aquatic system functioning. The aim of the present study is to investigate the effect of water and sediment contamination with pyrethroids and triazoles on fungal diversity. Water and sediment samples were seasonally collected along 2019 from water bodies representing Gharbeya and Qualubeya governorates. Concentrations of pyrethroids and triazoles were determined, and fungal species in water and sediment samples were molecularly identified. The results indicated that temperature and pH varied according to the season. Permethrin, deltamethrin, lambda-cyhalothrin and esfenvalerate showed the highest pyrethroids concentrations, whereas tetraconazole, tebuconazole and difenconazole were of the highest triazoles concentrations. <i>Aspergillus niger</i> was one of the most frequent species, in addition to <i>Trichoderma capillare, Fusarium oxysporum</i>, <i>Penicillium commune</i> and <i>Penicillium polonicum</i>. Principal component analysis indicated a positive correlation between temperature and different <i>Aspergillus</i> spp., and between pH and each of pyrethroids and triazoles. Moreover, a negative correlation was observed between triazoles and <i>Trichoderma asperellum</i>, <i>Penicillium griseofulvum</i> and <i>Aspergillus fumigatus</i>. In conclusion, contamination of water with pesticides affected fungal diversity. This disturbance in fungal assemblages might result in a reduction of some key organisms, or an increase and emergence of new pathogens.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 10","pages":"637-650"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-06-14DOI: 10.1080/03601234.2023.2220644
Akintunde Abiodun Ajulo, Princewill Chukwuma Asobia, Rodrigo Silva de Oliveira, Gustavo de Andrade Bezerra, Ariany Rosa Gonçalves, Marta Cristina Corsi de Filippi
Sheath blight (Rhizoctonia solani) causes significant yield losses in rice (Oryza sativa L.). Its sustainable management needs an efficient biocontrol agent. The objective was to screen bacterial isolates as an antagonist to R. solani and identify the most efficient ones as sheath blight suppressors under greenhouse conditions. Two assays (E1 and E2) were performed in a completely randomized design with three replications. E1 tested 21 bacterial isolates antagonists to R. solani in vitro. E2 was conducted under greenhouse conditions, with rice cultivar BRS Pampeira sown in plastic pots (7 kg) containing fertilized soil. Sixty old plants were inoculated with a segment of a toothpick containing fragments of R. solani, followed by spray inoculation of a bacterial suspension (108 CFU/mL). The severity of the disease was determined by calculating the relative lesion size formed on the colm. Isolates BRM32112 (Pseudomonas nitroreducens), BRM65929 (Priestia megaterium), and BRM65919 (Bacillus cereus) reduced R. solani colony radial growth by 92.8, 77.56, and 75.56%, respectively while BRM63523 (Serratia marcescens), BRM65923 and BRM65916 (P. megaterium) and BRM65919 (B. cereus) with 23.45, 23.37, 23.62, and 20.17 cm, respectively were effective at suppressing sheath blight in greenhouse, indicating their potential as a biofungicide for sheath blight suppression.
{"title":"Screening bacterial isolates for biocontrol of sheath blight in rice plants.","authors":"Akintunde Abiodun Ajulo, Princewill Chukwuma Asobia, Rodrigo Silva de Oliveira, Gustavo de Andrade Bezerra, Ariany Rosa Gonçalves, Marta Cristina Corsi de Filippi","doi":"10.1080/03601234.2023.2220644","DOIUrl":"10.1080/03601234.2023.2220644","url":null,"abstract":"<p><p>Sheath blight (<i>Rhizoctonia solani</i>) causes significant yield losses in rice (<i>Oryza sativa</i> L.). Its sustainable management needs an efficient biocontrol agent. The objective was to screen bacterial isolates as an antagonist to <i>R. solani</i> and identify the most efficient ones as sheath blight suppressors under greenhouse conditions. Two assays (E1 and E2) were performed in a completely randomized design with three replications. E1 tested 21 bacterial isolates antagonists to <i>R. solani in vitro</i>. E2 was conducted under greenhouse conditions, with rice cultivar BRS Pampeira sown in plastic pots (7 kg) containing fertilized soil. Sixty old plants were inoculated with a segment of a toothpick containing fragments of <i>R. solani</i>, followed by spray inoculation of a bacterial suspension (10<sup>8</sup> CFU/mL). The severity of the disease was determined by calculating the relative lesion size formed on the colm. Isolates BRM32112 (<i>Pseudomonas nitroreducens</i>), BRM65929 (<i>Priestia megaterium</i>), and BRM65919 (<i>Bacillus cereus</i>) reduced <i>R. solani</i> colony radial growth by 92.8, 77.56, and 75.56%, respectively while BRM63523 (<i>Serratia marcescens</i>), BRM65923 and BRM65916 (<i>P. megaterium</i>) and BRM65919 (<i>B. cereus</i>) with 23.45, 23.37, 23.62, and 20.17 cm, respectively were effective at suppressing sheath blight in greenhouse, indicating their potential as a biofungicide for sheath blight suppression.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 5","pages":"426-435"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9776152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study examined the effects of mesoporous silica nanoparticles (MSNs) on its adsorption capacity of aflatoxin B1 (AFB1). Moreover, the study evaluated the toxicity of MSNs with AFB1 using NIH3T3 cells and hemolysis test. The obtained MSNs were spherical, irregular-like in shape, having a mean size of 39.97 ± 7.85 nm and a BET surface area of 1195 m2/g. At 0.1 mg mL-1 concentration of MSN, the AFB1 adsorption capacity was 30%, which reached 70% when the MSN concentration increased to 2.0 mg mL-1. Our findings showed that AFB1 was adsorbed (∼67%) in the first few minutes on being in contact with MSNs, reaching an adsorption capacity of ∼70% after 15 min. Thereafter, the adsorption capacity remained constant in solution, demonstrating that the MSNs adsorbed toxins even beyond overnight. MSN treatment (0.5-2.0 mg mL-1) using NIH3T3 cells did not result in any reduction in cell viability. In addition, MSN treatment completely reversed the cytotoxic effect of AFB1 at all concentrations. Hemolysis test also revealed no hemolysis in MSNs evaluated alone and in those combined with AFB1. To the best of our knowledge, this study is the first to demonstrate that MSN can reduce cell toxicity produced by AFB1 due to its potential to adsorb mycotoxins.
{"title":"Mesoporous silica nanoparticles adsorb aflatoxin B<sub>1</sub> and reduce mycotoxin-induced cell damage.","authors":"Geovana Dagostim Savi, Elton Torres Zanoni, Rahisa Scussel, Emily da Silva Córneo, Bianca Guimarães Furtado, Domingos Lusitâneo Pier Macuvele, Janaína Nones, Paulo Emilio Feuser, Ricardo Andrez Machado-de-Ávila, Elidio Angioletto","doi":"10.1080/03601234.2022.2161251","DOIUrl":"https://doi.org/10.1080/03601234.2022.2161251","url":null,"abstract":"<p><p>The present study examined the effects of mesoporous silica nanoparticles (MSNs) on its adsorption capacity of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>). Moreover, the study evaluated the toxicity of MSNs with AFB<sub>1</sub> using NIH3T3 cells and hemolysis test. The obtained MSNs were spherical, irregular-like in shape, having a mean size of 39.97 ± 7.85 nm and a BET surface area of 1195 m<sup>2</sup>/g. At 0.1 mg mL<sup>-1</sup> concentration of MSN, the AFB<sub>1</sub> adsorption capacity was 30%, which reached 70% when the MSN concentration increased to 2.0 mg mL<sup>-1</sup>. Our findings showed that AFB<sub>1</sub> was adsorbed (∼67%) in the first few minutes on being in contact with MSNs, reaching an adsorption capacity of ∼70% after 15 min. Thereafter, the adsorption capacity remained constant in solution, demonstrating that the MSNs adsorbed toxins even beyond overnight. MSN treatment (0.5-2.0 mg mL<sup>-1</sup>) using NIH3T3 cells did not result in any reduction in cell viability. In addition, MSN treatment completely reversed the cytotoxic effect of AFB<sub>1</sub> at all concentrations. Hemolysis test also revealed no hemolysis in MSNs evaluated alone and in those combined with AFB<sub>1</sub>. To the best of our knowledge, this study is the first to demonstrate that MSN can reduce cell toxicity produced by AFB<sub>1</sub> due to its potential to adsorb mycotoxins.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 1","pages":"1-9"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9095588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aquatic organisms are exposed to chemical pesticides including glyphosate (Sharp 480 SL) and atrazine (Atraforce), two phytocidal molecules used for agriculture purposes in Benin. In this study, we assessed the acute toxicity of these two herbicides with emphasis on their histopathological effects on the liver of catfish Clarias gariepinus. One hundred and eighty juveniles of C. gariepinus (mean length 7.26 ± 0.59 cm and mean weight 5.21 ± 3.22 g) were exposed over 96 h to increasing concentrations of each phytocide. The values of 96 h-LC50 were 6.175 × 103 and 3.165 ppm, respectively for Sharp 480 SL and Atraforce. This indicates that Sharp 480 SL was nontoxic, while Atraforce displayed a moderate toxicity to C. gariepinus juveniles. During the tests, the behavioral responses (hyperexcitation, lethargy, loss of balance, discoloration of skin, etc.) that usually precede death were observed in exposed fishes, confirming the neurotoxicity of these phytocides. Histological alterations observed in liver of contaminated fishes were regressive changes, such as necrosis, hepatocyte vacuolation, nuclear degeneration, hepatocytes degeneration, sinusoids dilatation, etc. These results indicate that exposure to these herbicides had destructive effects on the liver of C. gariepinus.
{"title":"Evaluation of acute toxicity and histology effect on liver of glyphosate and atrazine in the African catfish <i>Clarias gariepinus</i> (Burchell 1822).","authors":"Prudencio Agbohessi, Laurence Olowo, Bodelaire Degila, Gisèle Houedjissi, Ibrahim Imorou Toko","doi":"10.1080/03601234.2022.2162797","DOIUrl":"10.1080/03601234.2022.2162797","url":null,"abstract":"<p><p>Aquatic organisms are exposed to chemical pesticides including glyphosate (Sharp 480 SL) and atrazine (Atraforce), two phytocidal molecules used for agriculture purposes in Benin. In this study, we assessed the acute toxicity of these two herbicides with emphasis on their histopathological effects on the liver of catfish <i>Clarias gariepinus.</i> One hundred and eighty juveniles of <i>C. gariepinus</i> (mean length 7.26 ± 0.59 cm and mean weight 5.21 ± 3.22 g) were exposed over 96 h to increasing concentrations of each phytocide. The values of 96 h-LC<sub>50</sub> were 6.175 × 10<sup>3</sup> and 3.165 ppm, respectively for Sharp 480 SL and Atraforce. This indicates that Sharp 480 SL was nontoxic, while Atraforce displayed a moderate toxicity to <i>C. gariepinus</i> juveniles. During the tests, the behavioral responses (hyperexcitation, lethargy, loss of balance, discoloration of skin, etc.) that usually precede death were observed in exposed fishes, confirming the neurotoxicity of these phytocides. Histological alterations observed in liver of contaminated fishes were regressive changes, such as necrosis, hepatocyte vacuolation, nuclear degeneration, hepatocytes degeneration, sinusoids dilatation, etc. These results indicate that exposure to these herbicides had destructive effects on the liver of <i>C. gariepinus</i>.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 1","pages":"21-30"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9102140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chlorpyrifos (CPF) is a widely used organophosphorus pesticide. Increasing evidence has shown that exposure to CPF in early life might induce neurodevelopmental disorders, but the pathogenesis remains uncertain. Synaptic plasticity plays a crucial role in neurodevelopment. This study aimed to investigate the effect of CPF on synaptic plasticity in hippocampal neurons and establish the cellular mechanism underlying these effects. Using CPF-exposed rat and primary hippocampal neurons model, we analyzed the impact of CPF on the synaptic morphology, the expression level of a presynaptic protein, a postsynaptic protein and ionotropic glutamate receptors (iGluRs), as well as the effects on the Wnt/β-catenin pathway. We found that the synapses were shortened, the spines were decreased, and the expression of synaptophysin (Syp), postsynaptic density-95 (PSD-95), GluN1, GluA1 and Wnt7a, as well as active β-catenin in primary hippocampal neurons was decreased. Our study suggests that CPF exposure induced dysregulation of synaptic plasticity in rat hippocampal neurons, which might provide novel information regarding the mechanism of CPF-induced neurodevelopmental disorders.
{"title":"Chlorpyrifos-induced dysregulation of synaptic plasticity in rat hippocampal neurons.","authors":"Wenjuan Zhou, Chen Zhang, Peipei Wang, Yuanying Deng, Hongmei Dai, Jing Tian, Guojiao Wu, Lingling Zhao","doi":"10.1080/03601234.2023.2171236","DOIUrl":"https://doi.org/10.1080/03601234.2023.2171236","url":null,"abstract":"<p><p>Chlorpyrifos (CPF) is a widely used organophosphorus pesticide. Increasing evidence has shown that exposure to CPF in early life might induce neurodevelopmental disorders, but the pathogenesis remains uncertain. Synaptic plasticity plays a crucial role in neurodevelopment. This study aimed to investigate the effect of CPF on synaptic plasticity in hippocampal neurons and establish the cellular mechanism underlying these effects. Using CPF-exposed rat and primary hippocampal neurons model, we analyzed the impact of CPF on the synaptic morphology, the expression level of a presynaptic protein, a postsynaptic protein and ionotropic glutamate receptors (iGluRs), as well as the effects on the Wnt/β-catenin pathway. We found that the synapses were shortened, the spines were decreased, and the expression of synaptophysin (Syp), postsynaptic density-95 (PSD-95), GluN1, GluA1 and Wnt7a, as well as active β-catenin in primary hippocampal neurons was decreased. Our study suggests that CPF exposure induced dysregulation of synaptic plasticity in rat hippocampal neurons, which might provide novel information regarding the mechanism of CPF-induced neurodevelopmental disorders.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 2","pages":"100-109"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9136991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1080/03601234.2023.2247942
Luwen Zhang, Lixin Xu, Lei Zhang, Yan Zhang, Yuxiang Chen
In this work, vermicompost was prepared with maize stover and cattle dung in ratios of 60:40 (VC1), 50:50 (VC2) and 40:60 (VC3), and the physicochemical properties of the vermicompost were related to the ratio of the raw materials used. The effect of the vermicomposts on the adsorption kinetics, adsorption isotherms and desorption of atrazine were investigated in unamended soil (S) and soil amended with 4% (w/w) of VC1(S-VC1), VC2(S-VC2) and VC3(S-VC3). The total organic carbon (TOC) content of VC1, VC2 and VC3 was 38.46, 37.33 and 34.47%, the HA content was 43.50, 42.22 and 39.28 g/kg, and the HA/FA ratios was 1.47, 0.44 and 0.83, respectively. The adsorption of atrazine on the soil, on the vermicompost and on soils amended with vermicompost followed a pseudo-second-order kinetic model. The Freundlich equation better fitted the adsorption isotherm of atrazine. The vermicomposts enhanced atrazine adsorption and decreased atrazine desorption. Correlation analysis showed that the TOC and HA were significantly positively correlated with Kf, which indicated that TOC and HA of the vermicomposts contributed significantly to the adsorption and desorption of atrazine. This study demonstrated that vermicomposts have great potential in the bioremediation of atrazine pollution and that their role is related to the raw materials used to prepare them.
{"title":"Adsorption-desorption characteristics of atrazine on soil and vermicompost prepared with different ratios of raw materials.","authors":"Luwen Zhang, Lixin Xu, Lei Zhang, Yan Zhang, Yuxiang Chen","doi":"10.1080/03601234.2023.2247942","DOIUrl":"https://doi.org/10.1080/03601234.2023.2247942","url":null,"abstract":"<p><p>In this work, vermicompost was prepared with maize stover and cattle dung in ratios of 60:40 (VC<sub>1</sub>), 50:50 (VC<sub>2</sub>) and 40:60 (VC<sub>3</sub>), and the physicochemical properties of the vermicompost were related to the ratio of the raw materials used. The effect of the vermicomposts on the adsorption kinetics, adsorption isotherms and desorption of atrazine were investigated in unamended soil (S) and soil amended with 4% (w/w) of VC<sub>1</sub>(S-VC<sub>1</sub>), VC<sub>2</sub>(S-VC<sub>2</sub>) and VC<sub>3</sub>(S-VC<sub>3</sub>). The total organic carbon (TOC) content of VC<sub>1</sub>, VC<sub>2</sub> and VC<sub>3</sub> was 38.46, 37.33 and 34.47%, the HA content was 43.50, 42.22 and 39.28 g/kg, and the HA/FA ratios was 1.47, 0.44 and 0.83, respectively. The adsorption of atrazine on the soil, on the vermicompost and on soils amended with vermicompost followed a pseudo-second-order kinetic model. The Freundlich equation better fitted the adsorption isotherm of atrazine. The vermicomposts enhanced atrazine adsorption and decreased atrazine desorption. Correlation analysis showed that the TOC and HA were significantly positively correlated with <i>K<sub>f</sub></i>, which indicated that TOC and HA of the vermicomposts contributed significantly to the adsorption and desorption of atrazine. This study demonstrated that vermicomposts have great potential in the bioremediation of atrazine pollution and that their role is related to the raw materials used to prepare them.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 9","pages":"583-593"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10101347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-02-12DOI: 10.1080/03601234.2023.2177462
Ana Carolina Oliveira Chapeta, Luiz Ricardo Dos Santos Tozin, Amanda Dos Santos Souza, Milena Gonçalves Costa, Jéssica Ferreira Lourenço Leal, Camila Ferreira de Pinho
Euphorbia hirta L. is a weed species that is tolerant to the most used herbicide in agriculture, glyphosate. The anatomical characteristics of plants influence the processes of absorption and translocation of herbicides. The objective of this work was to characterize the anatomy of the aerial vegetative axis (leaves and stem) of E. hirta, to support the establishment of strategies for better control of this species with herbicides. The plants were grown in a greenhouse under controlled conditions. When they reached sizes between 8 and 12 cm, samples of stems and leaves were collected, fixed in FAA 50, and stored in 70% ethanol. Subsequently, the samples were processed following usual light microscopy techniques. In cross-section, the stem of E. hirta has a circular shape. The leaf epidermis is uniseriate composed of isodiametric cells of compact arrangement and with the presence of multicellular trichomes and anthocyanin. As for the morphometric parameters evaluated, the young leaves have a lower thickness in the abaxial epidermis. Based on the anatomical characteristics observed in E. hirta, the main barriers that can act in the absorption of herbicides are the high hairiness and the high content of anthocyanin in the epidermal cells.
大戟属(Euphorbia hirta L.)是一种杂草,对农业中最常用的除草剂草甘膦具有耐受性。植物的解剖特征会影响除草剂的吸收和转移过程。这项工作的目的是描述 E. hirta 的气生无性轴(叶片和茎)的解剖特征,以帮助制定更好地用除草剂控制该物种的策略。这些植物是在受控条件下在温室中生长的。当植株长到 8 到 12 厘米时,采集茎叶样本,用 FAA 50 固定,并保存在 70% 的乙醇中。随后,按照通常的光学显微镜技术对样本进行处理。从横截面看,E. hirta 的茎呈圆形。叶表皮是单列的,由排列紧密的等轴细胞组成,存在多细胞毛状体和花青素。在形态参数评估方面,幼叶背面表皮厚度较低。根据在 E. hirta 观察到的解剖特征,表皮细胞中的高毛发和高含量花青素是除草剂吸收的主要障碍。
{"title":"Leaf and stem anatomical characterization of <i>Euphorbia hirta</i> L., a tolerant species to glyphosate.","authors":"Ana Carolina Oliveira Chapeta, Luiz Ricardo Dos Santos Tozin, Amanda Dos Santos Souza, Milena Gonçalves Costa, Jéssica Ferreira Lourenço Leal, Camila Ferreira de Pinho","doi":"10.1080/03601234.2023.2177462","DOIUrl":"10.1080/03601234.2023.2177462","url":null,"abstract":"<p><p><i>Euphorbia hirta</i> L. is a weed species that is tolerant to the most used herbicide in agriculture, glyphosate. The anatomical characteristics of plants influence the processes of absorption and translocation of herbicides. The objective of this work was to characterize the anatomy of the aerial vegetative axis (leaves and stem) of <i>E. hirta</i>, to support the establishment of strategies for better control of this species with herbicides. The plants were grown in a greenhouse under controlled conditions. When they reached sizes between 8 and 12 cm, samples of stems and leaves were collected, fixed in FAA 50, and stored in 70% ethanol. Subsequently, the samples were processed following usual light microscopy techniques. In cross-section, the stem of <i>E. hirta</i> has a circular shape. The leaf epidermis is uniseriate composed of isodiametric cells of compact arrangement and with the presence of multicellular trichomes and anthocyanin. As for the morphometric parameters evaluated, the young leaves have a lower thickness in the abaxial epidermis. Based on the anatomical characteristics observed in <i>E. hirta</i>, the main barriers that can act in the absorption of herbicides are the high hairiness and the high content of anthocyanin in the epidermal cells.</p>","PeriodicalId":15720,"journal":{"name":"Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes","volume":"58 3","pages":"203-209"},"PeriodicalIF":2.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9579394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}