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Use of 16S rDNA Sequencing to Determine Procaryotic Diversity of a Remote Aviation Fuel-Polluted Lentic Ecosystem in Ibeno, Nigeria 利用16S rDNA测序测定尼日利亚伊贝诺偏远航空燃料污染生态系统的原核生物多样性
Pub Date : 2017-07-22 DOI: 10.4172/2161-0525.1000493
I. Udotong, M. Uko, J. Udotong
Ibeno, the operational base of Mobil Producing Nigeria Unlimited, a subsidiary of ExxonMobil, Nigeria remains one of the most impacted communities by oil and gas activities in the Niger Delta region of Nigeria. Lotic and lentic systems in the region which residents rely on, receive petroleum hydrocarbon inputs almost daily due to oil spills and oily wastes discharges from operators and bunkering activities. This research was carried out to determine the prokaryotic diversity in a remote aviation fuel-polluted lentic ecosystem after 16 years of pollution using metagenomic approaches. DNA extraction from the water samples was carried out using MoBio DNA extraction Kits following the manufacturer’s instructions. Extracted DNA fragments were quantified using picogreen and by recording their UV absorption spectra using NanoDrop spectrophotometer. 16S rDNA sequencing was carried out on a Miseq Illumina sequencing platform and Quantitative Insight Into Microbial Ecology (QIIME) bioinformatics pipeline. Analyses revealed the dominance of bacterial and archaeal communities in both polluted and unpolluted water samples. The polluted sample had 93.83% bacterial and 3.43% archaeal population while the control sample revealed 58.05% bacterial and 39.69% archaeal population. Dominant bacterial phyla from the polluted samples were Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria, and Chloroflexi while dominant phyla in the unpolluted samples were Proteobacteria, Firmicutes and Actinobacteria. Dominant archaeal phyla from both polluted and unpolluted waters were Euryarchaeota and Crenarchaeota. The use of 16S rDNA metagenomic approach revealed a wide variety of bacterial and archaeal diversity from both polluted and control sites, thus revealing the true ecological status of both sites.
Ibeno是埃克森美孚的子公司Mobil production Nigeria Unlimited的运营基地,是尼日利亚尼日尔三角洲地区受油气活动影响最大的地区之一。由于石油泄漏和油类废物的排放以及加油活动,该地区居民所依赖的油气系统几乎每天都要接收石油烃的输入。本研究采用宏基因组方法确定了一个偏远的航空燃料污染的生物生态系统经过16年污染后的原核生物多样性。按照制造商的说明,使用MoBio DNA提取试剂盒从水样中提取DNA。提取的DNA片段用picogreen定量,用NanoDrop分光光度计记录其紫外吸收光谱。16S rDNA测序在Miseq Illumina测序平台和QIIME生物信息学管道上进行。分析表明,在污染和未污染的水样中,细菌和古细菌群落都占主导地位。污染样品细菌总数为93.83%,古菌总数为3.43%;对照样品细菌总数为58.05%,古菌总数为39.69%。污染样品的优势菌门为变形菌门、厚壁菌门、放线菌门、蓝藻门和氯霉素门,未污染样品的优势菌门为变形菌门、厚壁菌门和放线菌门。污染水体和未污染水体的优势古菌门类均为Euryarchaeota和Crenarchaeota。利用16S rDNA宏基因组方法揭示了污染点和对照点细菌和古细菌的多样性,从而揭示了污染点和对照点的真实生态状况。
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引用次数: 0
Evaluation of Selected Numerical Weather Prediction Models for a Case of Widespread Rainfall over Central and Southern Nigeria 对尼日利亚中部和南部大范围降雨的若干数值天气预报模式的评价
Pub Date : 2017-07-20 DOI: 10.4172/2161-0525.1000491
Chibuzo N Agogbuo, M. Nwagbara, E. Bekele, A. Olusegun
Precipitation forecasts from four Numerical Weather Prediction (NWP) models are evaluated for a case of widespread rainfall event over Central and Southern Nigeria on the 21st of March 2015. The four models evaluated are the European Centre for Medium Range Weather Forecast (ECMWF) with a resolution of 25 km, The UKMET model 20 km, NCEP Global Forecast System (GFS) 50 km and the Weather Research and Forecast Model (WRF) with 10 km resolution. Precipitation forecasts are compared with observed precipitation at station and gridded observation points for different rainfall amount thresholds using the Method of Objective-based Diagnostic Evaluation (MODE), Grid statistics and Point Statistics. The global models ECMWF, UKMET and GFS underestimated the rainfall amount when compared to the WRF regional model. The global models recorded a critical success index (CSI) of less than 10% while the regional WRF model recorded a critical success index of 40% for rainfall amount greater than 25 mm. MODE analysis showed that the WRF model also recorded a 93% relationship between observed and forecast precipitation events of 21st March 2015 over Nigeria when compared with the ECMWF, UKMET and GFS models which showed 88%, 88% and 87% relationship respectively. Our findings suggest that dynamically downscaling a global model using the WRF model added value and gave a better skill of precipitation forecast for the event under study.
对2015年3月21日尼日利亚中南部大范围降雨事件的四个数值天气预报模式的降水预报进行了评估。评估的四个模式分别是欧洲中期天气预报中心(ECMWF),分辨率为25公里,UKMET模式为20公里,NCEP全球预报系统(GFS)为50公里,天气研究与预报模式(WRF)为10公里。采用基于目标的诊断评价方法(MODE)、网格统计和点统计方法,对不同降雨量阈值的降水预报与站点和网格观测点的观测降水进行比较。与WRF区域模式相比,全球模式ECMWF、UKMET和GFS低估了降雨量。对于大于25 mm的降雨量,全球模式的临界成功指数(CSI)低于10%,而区域WRF模式的临界成功指数为40%。MODE分析显示,WRF模式记录的2015年3月21日尼日利亚降水事件与预报事件的相关性为93%,而ECMWF、UKMET和GFS模式的相关性分别为88%、88%和87%。我们的研究结果表明,使用WRF模式的动态降尺度全球模式增加了研究事件的降水预报价值,并提供了更好的降水预报技能。
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引用次数: 3
Hydrochemical and Geochemical Characteristics of Geothermal Water in Gedong Area of Guizhou Province 贵州葛东地区地热水水化学地球化学特征
Pub Date : 2017-07-10 DOI: 10.4172/2161-0525.1000488
Ban Wen-tao, Li Bo
Geothermal energy is a green and renewable energy source. There are abundant geothermal resources in Gedong area of Guizhou Province, China. The Geothermal resources are controlled by regional structure. The shape of heat storage is zonal distribution. Inorder to know more about the characteristics of banded thermal reservoirs and provide reference for the development and utilization of banded thermal reservoirs in Qiandongnan area of Guizhou, on the basis of fully understanding the geothermal geological conditions in the Gedong area of Guizhou, the samples of geothermal fluid were collected and the hydrochemistry, hydrogen and oxygen isotopes of regional geothermal water were analyzed. The result shows that regional geothermal water circulation is very deep. The circulation depth of geothermal water is at least 1330 m. Ground water is heated continuously by large geothermal flow during circulation and finally becomes geothermal water. The source of geothermal water supply is meteoric water and its lower limit of recharge height is 926 m. The characteristics of hydrogen and oxygen isotopes of geothermal water in different geothermal wells (Springs) are different, and the main factor is elevation. The chemical composition of geothermal water is controlled by the surrounding rock of geothermal water, the dissolved specific mineral of geothermal water and the circulation of geothermal water are difference. Geothermal water in different geothermal wells (Springs) can be divided into two groups. The division is based on whether geothermal water reaches mineral equilibrium and whether it is mixed with shallow ground water.
地热能是一种绿色可再生能源。中国贵州省葛东地区地热资源丰富。地热资源受区域构造控制。蓄热形态呈带状分布。为进一步了解贵州黔东南地区带状热储特征,为带状热储开发利用提供参考,在充分了解贵州葛东地区地热地质条件的基础上,采集了地热流体样品,对区域地热水进行了水化学、氢、氧同位素分析。结果表明,区域地热水循环非常深。地热水循环深度至少为1330 m。地下水在循环过程中受到大地热流的不断加热,最终成为地热水。地热水补给源为大气降水,补给高度下限为926 m。不同地热井(泉)地热水氢、氧同位素特征不同,其主要影响因素是高程。地热水的化学成分受地热水围岩的控制,地热水的溶解比矿物和地热水的循环有差异。不同地热井(泉)中的地热水可分为两类。划分依据是地热水是否达到矿物平衡以及是否与浅层地下水混合。
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引用次数: 2
Ecological and Functional Evaluation of Species Candidate for Heavy Metals Phytoremediation in SIN Porto Torres (Sardinia, Italy) 意大利撒丁岛sinporto Torres重金属植物修复候选物种生态与功能评价
Pub Date : 2017-06-26 DOI: 10.4172/2161-0525.1000484
C. Guarino, R. Sciarrillo
The pollution of metals and metalloids in soils caused by industrial activities is a major issue and invests the whole planet earth. The area we investigated is present in the Petrochemical of Porto Torres (Sardinia-Italy), which has an extension of about 1100 ha, is located along the northwest coast of Sardinia, about 1.8 km west of the town and with a surface contamination (0-50 cm depth) from As, Tl and V. The aim of this study is the investigation of spontaneous species present in the areas and the possibility to find some species among those useful to the phytoremediation. We also evaluate (mesocosm) some species that spontaneously live in the Porto Torres area but not on the area under consideration such as Dittrichia viscosa and Piptatherum miliaceum which, according to literature data, showed a good attitude towards phytoremediation. In conclusion, Dittrichia viscosa, Piptatherum miliaceum and Bromus madritensis are good candidates to build a phytoremediation design.
工业活动对土壤中金属和类金属的污染是一个重大问题,对整个地球都有影响。我们调查的区域是在波尔图的石化托雷斯(Sardinia-Italy)的延伸约1100公顷,位于撒丁岛西北海岸,镇以西约1.8公里,表面污染(0-50厘米深度),Tl和v .本研究的目的是调查自然物种的出现在地区和可能发现其中一些物种有用植物修复。我们还对一些自发生长在波尔图托雷斯地区而非考虑区域的物种进行了(中观)评价,如粘胶水蚤(Dittrichia viscosa)和水蚤(Piptatherum miliaceum),根据文献资料,这些物种对植物修复表现出良好的态度。综上所述,粘毛滴虫(Dittrichia粘毛滴虫)、百里吸毛滴虫(Piptatherum milium)和马蝇(Bromus madritensis)是构建植物修复设计的理想选择。
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引用次数: 2
Pollution Threatens Human Fertility: It is Time to Make Our Gametes Great Again 污染威胁着人类的生育能力:是时候让我们的配子再次伟大了
Pub Date : 2017-06-26 DOI: 10.4172/2161-0525.1000482
J. Moreau, N. Gatimel, P. Vabre, J. Parinaud, R. Leandri
Historical examples of environmental pollution in wildlife have witnessed that mammalian reproduction is a process very highly sensitive to environmental toxins. Such example also exists in humans: it has been shown that breast-fed boys from women exposed to dioxins during the Seveso explosion have permanent reduced sperm quality. Those caricatural accidents have drawn attention on the possible links between environmental toxics and human reproduction and paved the way for studying the effects of much more insidious, chronic exposure [1-3]. The effects of toxics not only concern the exposed individuals, but also their progeny through transplacental impacts and/or epigenetic modifications with potential transgenerational inheritance, both leading to an actual problem of public health. Toxics can act on spermatogenesis as well as oogenesis, but also on embryonic and fetal development [4,5].
野生动物环境污染的历史实例表明,哺乳动物的繁殖是一个对环境毒素非常敏感的过程。这样的例子也存在于人类身上:有证据表明,在Seveso爆炸期间暴露于二恶英的妇女的母乳喂养的男孩会永久性地降低精子质量。这些具有讽刺意味的事故引起了人们对环境有毒物质与人类生殖之间可能存在的联系的关注,并为研究更阴险的慢性接触的影响铺平了道路[1-3]。有毒物质的影响不仅涉及接触者,还涉及他们的后代,通过经胎盘影响和/或具有潜在跨代遗传的表观遗传修饰,两者都导致实际的公共卫生问题。有毒物质不仅可以影响精子的发生,还可以影响胚胎和胎儿的发育[4,5]。
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引用次数: 0
Occurrence and Environmental Features of Heavy Metal Elements of Coal from Coalfield in the Southeast Shanxi 晋东南煤田煤中重金属元素赋存状态及环境特征
Pub Date : 2017-06-22 DOI: 10.4172/2161-0525.1000483
Duanle Li, Dong Wang, Yi Wang, Cai-fu Ren, N.-H. Yuan
The coal was sampled from No.15 coal seam in coalfield of southeast Shanxi. The Hg, As, Se, Cr and Pb occurrence in coal seam and environmental features of the heavy metal elements of coal were studied by X-ray fluorescence spectrometry (ARL Quant 'X), X-ray diffraction (XRD) and inductively couple-plasma mass spectrometry (ICP-MS). Environmental features, such as heavy metal element concentration, distribution and occurrence mode, etc., were studied by systematic determination of element concentration, inorganic element and mineral composition in coal. The results showed that the coal from No.15 coal seam had the mercury (Hg) concentration between 0.44 and 0.268 μg/g, and average Hg concentration of 0.11 μg/g; arsenic (As) concentration between 0.31 and 0.91 μg/g, 0.61 μg/g on average; selenium (Se) concentration between 0.94 and 3.56 μg/g, 2.45 μg/g on average; chromium (Cr) concentration between 9.54 and 24.58 μg/g, 17.06 μg/g on average; lead (Pb) concentration between 10.23 and 23.56 μg/g, 18.13 μg/g on average. The comparison with the Clark value showed Se was significantly enriched, As and Cr were lost, and Hg and Pb were normal. Compared with the coal from other counties and regions both at home and abroad, as was lost and other elements were normal. The elements irregularly distributed vertically. In spite of different element concentrations in adjacent coal seams, no significant enrichment occurred. The target elements mainly occurred in the form of inorganic matters except As. Hg was mainly concentrated in pyrite and clay minerals. Se, Cr and Pb were mainly concentrated in kaolinite, and Se was mainly concentrated in illite. Because of different occurrences or different forms in one mineral, the Hg, As, Se, Cr, and Pb loss rates were 99.43%, 85.91%, 77.89%, 73.81%, and 68.85% at 815°C, respectively.
煤样采自晋东南煤田15号煤层。采用X射线荧光光谱法(ARL Quant’X)、X射线衍射法(XRD)和电感耦合等离子体质谱法(ICP-MS)研究了煤层中Hg、As、Se、Cr、Pb的赋存状态及煤中重金属元素的环境特征。通过对煤中元素浓度、无机元素和矿物组成的系统测定,研究了煤中重金属元素浓度、分布和赋存方式等环境特征。结果表明:15煤层煤中汞(Hg)浓度为0.44 ~ 0.268 μg/g,平均汞(Hg)浓度为0.11 μg/g;砷(As)浓度在0.31 ~ 0.91 μg/g之间,平均0.61 μg/g;硒(Se)浓度在0.94 ~ 3.56 μg/g之间,平均为2.45 μg/g;铬(Cr)浓度在9.54 ~ 24.58 μg/g之间,平均为17.06 μg/g;铅(Pb)浓度在10.23 ~ 23.56 μg/g之间,平均18.13 μg/g。与Clark值比较,硒显著富集,砷、铬丢失,汞、铅正常。与国内外其他国家和地区的煤相比,as损失,其他元素正常。元素不规则地垂直分布。相邻煤层虽然元素含量不同,但均未发生明显富集。靶元素除As外主要以无机物形式存在。汞主要富集在黄铁矿和粘土矿物中。硒、铬、铅主要富集在高岭石中,硒主要富集在伊利石中。在815℃时,由于同一矿物的不同赋存状态或不同形态,Hg、As、Se、Cr和Pb的损失率分别为99.43%、85.91%、77.89%、73.81%和68.85%。
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引用次数: 0
Statistical Analysis of Corrosion Inhibition of Water Hyacinth on Mild Steel in an Acidic Medium 水葫芦在酸性介质中对低碳钢缓蚀性能的统计分析
Pub Date : 2017-06-20 DOI: 10.4172/2161-0525.1000481
F. Omoruwou, Okewale Ao, Owabor Cn
This present work investigated surface response modeling and optimization of corrosion inhibition of water hyacinth on mild steel surface in an acidic medium. This was achieved using the central composite design (CCD) experimental design. Response Surface Methodology was used to assess the effects of experimental process variables that influenced rate of corrosion, and for searching of optimum combinations of factors. The rate of corrosion on mild steel surface was study using weight loss method. The optimum process variables obtained from the quadratic model developed were 1.50 g/l inhibitor’s concentration, 8 hours exposure time, and temperature of 60°C with a predicted inhibitor’s efficiency value of 82.89%. The experimental result obtained from optimum value validation was 81.5% and the predicted optimum value was adequately represented. The mild steel corrosion inhibition was achieved through the double bond adsorption of carbonyl group existing in the inhibitor phytochemical constituent.
本文研究了酸性介质中水葫芦对低碳钢表面缓蚀的表面响应建模及优化。这是通过中心复合设计(CCD)实验设计实现的。采用响应面法评估了影响腐蚀速率的实验工艺变量的影响,并寻找了各因素的最佳组合。采用失重法研究了低碳钢表面的腐蚀速率。通过建立的二次模型得到最佳工艺变量为1.50 g/l,暴露时间为8 h,温度为60°C,预测抑制剂的效率值为82.89%。最优值验证的实验结果为81.5%,预测的最优值得到了充分的代表。缓蚀剂是通过植物化学成分中羰基的双键吸附实现对低碳钢的缓蚀作用。
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引用次数: 7
Microevolution on Anthropogenically Changed Areas on the Example of Biscutella laevigata Plants from Calamine Waste Heap in Poland 人为变化地区的微进化——以波兰炉甘石堆中的laevigata植物为例
Pub Date : 2017-06-19 DOI: 10.4172/2161-0525.1000479
M. Wierzbicka, M. Pielichowska, Olga Bemowska-Kałabun, P. Wąsowicz
In the era of increasing environmental pollution, microevolutionary processes occurring in plants inhabiting anthropogenic areas play a special role. With time, these processes may lead to formation of new plant species. A good example of occurrence of microevolutionary processes on anthropogenically altered areas is the metallophyte Biscutella laevigata L. The studies have shown the existence of significant morphological, anatomical and physiological differences between two groups of the B. laevigata populations occurring in Poland – the population of calamine waste heaps in BolesA…‚aw near Olkusz (Silesian Upland) and the population inhabiting the Tatra Mountains (Western Carpathians). The demonstrated differences are the adaptation (hereditary characteristics) of the plants to the unfavorable conditions of the calamine waste heap, i. a. high concentration of heavy metals in the soil. The research has also shown theexistence of significant differences between these two groups of populations – both at the genetic and morphological levels (a clonal form of vegetative propagation, removal of heavy metals by the oldest and drying leaves, a zinc tolerant species, trichomes accumulating metals, metal detoxification at the cellular level). The demonstrated differences between the zinc-lead (calamine) waste heap population and the Tatra Mountains population indicate the existence of the new subspecies of Biscutella laevigata subsp. woycickii on the heaps in Boleslaw, in Poland.
在环境污染日益严重的时代,人类活动区域植物的微进化过程发挥着特殊的作用。随着时间的推移,这些过程可能导致新的植物物种的形成。在人为改变的地区发生微进化过程的一个很好的例子是laevigata L.的金属植物。研究表明,在波兰发生的两组B. laevigata种群之间存在显著的形态、解剖和生理差异——BolesA…Â, aw附近Olkusz(西里西亚高地)的炉甘石废物堆种群和居住在Tatra山脉(西喀尔巴阡山脉)的种群。所显示的差异是植物对炉甘石废物堆不利条件的适应性(遗传特征),即土壤中重金属浓度高。研究还表明,这两组种群之间存在显著差异——在遗传和形态水平上(无性繁殖的克隆形式,最老和干燥的叶子去除重金属,耐锌物种,毛状体积累金属,细胞水平上的金属解毒)。锌铅(炉甘石)废物堆种群与塔特拉山种群之间的差异表明存在新的laevigata亚种。woycickii在波兰波尔斯瓦夫的垃圾堆上。
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引用次数: 3
Energy Paradox: Simulation and Policy Recommendations for the Case of Solar Water Heater 能源悖论:太阳能热水器案例的模拟与政策建议
Pub Date : 2017-06-19 DOI: 10.4172/2161-0525.1000480
Omar Jridi
The objective of this paper is crystallized around the explanation of the energy paradox, that of the non adoption of cost-effective energy-saving investments. The latter is mutually driven by two major factors. Uncertainty future benefits of energy savings, which we assume that they follow a geometric Brownian motion. In this stochastic process, we take into account the lower costs of efficient equipment as a result of learning by doing. To affirm the robustness of the model, we generate simulation results for the case of solar water heaters. The minimum rate of return required by households reached 18.81%. Beyond that, the model allows the simulation of the effects of the instruments of energy policy oriented to the promotion of the adoption of this equipment. We urge policymaker’s ineffectiveness of the policy of subsidy to the purchase of energy-saving equipment and the effectiveness of energy taxation policy. The combination of these two instruments amplifies the adoptions of these devices and generates very positive externalities in terms of energy saving and emission reduction of greenhouse gases.
本文的目标是围绕能源悖论的解释,即不采用具有成本效益的节能投资。后者是由两个主要因素共同推动的。未来节能效益的不确定性,我们假设它们遵循几何布朗运动。在这个随机过程中,我们考虑了由于边做边学而获得高效设备的较低成本。为了验证模型的鲁棒性,我们给出了太阳能热水器的仿真结果。住户要求的最低收益率为18.81%。除此之外,该模型还可以模拟旨在促进采用这种设备的能源政策手段的效果。我们对节能设备购置补贴政策的有效性和能源税收政策的有效性提出了质疑。这两种工具的结合扩大了这些设备的采用,并在节约能源和减少温室气体排放方面产生了非常积极的外部性。
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引用次数: 0
Effect of Meteorological Variables on Air Pollutants Variation in Arid Climates 气象变量对干旱气候下大气污染物变化的影响
Pub Date : 2017-06-19 DOI: 10.4172/2161-0525.1000478
J. Radaideh
Air quality and climatic factors are closely linked through atmospheric chemical reactions and dynamic processes. This work based on a study conducted over an area of industrial plant located in Saudi Arabia during three consecutive weeks with noticeable varying meteorological parameters in the last week of survey with the main objective to examine the role of climatic factors on differences in air pollutant concentration. A systematic analysis of air pollutants including TVOC, CO, SO2, NO2, and O3 and meteorological parameters including temperature, wind speed (WS), and relative humanity (RH) was conducted for a continuous period of 3 weeks from March to mid-April 2015. Acquired results indicate most of observed key air pollutants increase with increase in relative humidity, except NO2, which experiences a decrease in concentrations simultaneous with increasing relative humidity. This survey study suggests that concentrations of TVOC concentrations increase by 201%, CO by 15.2%, SO2 by 21.6% and ozone by 16% as a direct contribution to relative humidity change. While NO2 experiences a decrease by 53.4% as a result of increase in relative humidity. Ozone exhibits only slightly spatial variation due to sudden change of meteorological variables.
空气质量和气候因素通过大气化学反应和动态过程紧密联系在一起。这项工作基于对位于沙特阿拉伯的一个工业工厂区域进行的连续三周的研究,在调查的最后一周有明显的气象参数变化,主要目的是检查气候因素对空气污染物浓度差异的作用。2015年3月至4月中旬连续3周,系统分析了TVOC、CO、SO2、NO2、O3等大气污染物和气温、风速(WS)、相对湿度(RH)等气象参数。实测结果表明,除NO2浓度随相对湿度的增加而降低外,其余主要大气污染物均随相对湿度的增加而增加。该调查研究表明,TVOC浓度增加了201%,CO增加了15.2%,SO2增加了21.6%,臭氧增加了16%,这是相对湿度变化的直接贡献。而NO2则因相对湿度的增加而减少53.4%。由于气象变量的突然变化,臭氧仅表现出轻微的空间变化。
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引用次数: 25
期刊
Journal of Environmental and Analytical Toxicology
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