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Forensic analysis of cigarette tipping papers using ATR-FTIR spectroscopy and chemometrics 利用ATR-FTIR光谱和化学计量学对卷烟纸进行法医分析。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-17 DOI: 10.1111/1556-4029.70138
Nisha Rani MSc, Chongtham Nimi MSc, Rajinder Singh PhD

Cigarettes are addictive in nature and smokers over time develop a preference toward smoking a particular brand. Therefore, identification and discrimination of different brands could be important during forensic investigations. It could serve as corroborative evidence not only in cases of physical and sexual assaults, theft, burglary, and dacoity but also in cases of illegal trading and smuggling of illicit and counterfeit cigarettes. Whenever a cigarette (smoked or unsmoked) is recovered as evidence, the tipping paper would always be present as it is a part of cigarette butts. Thus, in the present study, 21 brands of cigarette tipping paper have been analyzed by conjugating attenuated total reflectance – Fourier transform infrared spectroscopy with chemometrics for brand discrimination and determination of geographical origin. Brand discrimination was performed using the support vector machine method and samples were discriminated with 100% training and validation accuracy. External validation was also performed where normal white and brown papers have been differentiated from tipping paper with 100% accuracy. Additionally, the geographical origin of samples was determined through PLS-DA with an R-square value of 0.998. Smoked samples were successfully linked with the unsmoked sample when projected to the already-built SVM model. 100% accuracy was achieved till 1 week; however, the accuracy was reduced to 71.42% after 1 week. This study proposes an alternative approach for analyzing cigarettes in a nondestructive and eco-friendly manner to highlight and amplify the potential of cigarettes as evidence.

香烟本质上是会上瘾的,随着时间的推移,吸烟者会对某一特定品牌的香烟产生偏好。因此,识别和区分不同的品牌在法医调查中可能是重要的。它不仅可以在人身攻击和性攻击、盗窃、入室盗窃和抢劫案件中,而且可以在非法交易和走私非法和假冒香烟案件中作为确凿证据。每当一支香烟(无论是抽过的还是未抽过的)被作为证据收回时,小费纸总会出现,因为它是烟头的一部分。因此,在本研究中,用衰减全反射-傅立叶变换红外光谱与化学计量学相结合的方法对21个品牌的卷烟纸进行了分析,用于品牌识别和地理来源的确定。采用支持向量机方法进行品牌识别,样本识别的训练和验证准确率均为100%。外部验证也进行了正常的白色和棕色的纸已经区分与小费纸100%的准确性。另外,通过PLS-DA法确定了样品的地理来源,r平方值为0.998。将烟熏样本投影到已建立的SVM模型中,成功地将烟熏样本与未烟熏样本连接起来。到第1周,准确率达到100%;但1周后,准确率降至71.42%。本研究提出了一种以非破坏性和环保的方式分析香烟的替代方法,以突出和扩大香烟作为证据的潜力。
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引用次数: 0
Source discrimination of colchicine based on carbon stable isotope analysis 基于碳稳定同位素分析的秋水仙碱来源鉴别。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-17 DOI: 10.1111/1556-4029.70134
Hanyang Zheng MSc, Jun Zhu PhD, Zhuotong Cai MSc, Zhaowei Jie PhD, Wei Wang PhD, Hanyu Zhang MSc, Can Hu PhD, Hongling Guo PhD, Hongcheng Mei PhD

Colchicine, a highly toxic alkaloid, has been frequently employed as a poisoning agent in criminal cases. Toxicant source tracing represents a critical research direction in forensic science, where the discrimination of colchicine origins holds particular significance for chemical fingerprint identification in poisoning incidents. The stable isotopic signature of colchicine serves as a crucial indicator for source comparison and traceability. However, its complexity of structure and high molecular weight present substantial challenges for precise stable isotope analysis. To enhance traceability capabilities, a method for the analysis of carbon stable isotope of colchicine was established using gas chromatography–combustion–isotope ratio mass spectrometry (GC-C-IRMS). Through systematic optimization of experimental conditions, we resolved incomplete oxidation issues in the combustion reactor caused by inherent chemical properties of colchicine, achieving precise measurements with a standard deviation below 0.3‰. Method validation confirmed that storage conditions and matrix effects exerted no significant impact on carbon stable isotope ratio determinations. Applied to colchicine from three distinct sources, this method demonstrated effective source discrimination through δ13C values. The established analytical protocol proves reliable and robust for colchicine stable carbon isotope analysis, thereby significantly increasing its traceability potential in forensic science.

秋水仙碱是一种剧毒生物碱,在刑事案件中经常被用作投毒剂。毒物来源溯源是法医学的一个重要研究方向,秋水仙碱来源的鉴别对中毒事件的化学指纹鉴定具有重要意义。秋水仙碱的稳定同位素特征是来源比较和溯源的重要指标。然而,其结构的复杂性和高分子量给精确的稳定同位素分析带来了很大的挑战。为提高秋水仙碱碳稳定同位素的溯源能力,建立了气相色谱-燃烧-同位素比质谱(GC-C-IRMS)分析秋水仙碱碳稳定同位素的方法。通过系统优化实验条件,解决了秋水仙碱固有化学性质导致的燃烧反应器不完全氧化问题,实现了标准偏差在0.3‰以下的精确测量。方法验证证实,储存条件和基质效应对碳稳定同位素比值测定没有显著影响。该方法通过δ13C值对三种不同来源的秋水仙碱进行了有效的源鉴别。所建立的秋水仙碱稳定碳同位素分析方法可靠、稳健,大大提高了秋水仙碱在法医学中的可追溯性。
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引用次数: 0
Metastatic seminoma: Initial diagnosis at autopsy due to fatal pulmonary thromboembolism 转移性精原细胞瘤:由于致命的肺血栓栓塞而在尸检中初步诊断。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-17 DOI: 10.1111/1556-4029.70137
Nadia Lee MBBS, George Paul MBBS, DMJ

Testicular cancer is the most common solid organ malignancy among young males, with a good survival rate if detected in a timely manner. We describe a case where a previously well young adult male died suddenly due to previously undiagnosed metastatic testicular cancer with retroperitoneal lymphadenopathy, resulting in compression and invasion of the inferior vena cava and development of pulmonary thromboembolism. This was attributed to both mechanical compression of the inferior vena cava and the prothrombotic effect of cancer. This is an unusual presentation of seminoma, where diagnosis was only made upon discovery of the pulmonary thromboembolism at autopsy.

睾丸癌是年轻男性中最常见的实体器官恶性肿瘤,如果及时发现,生存率很高。我们描述了一个病例,以前良好的年轻成年男性突然死亡,由于以前未确诊转移性睾丸癌伴腹膜后淋巴结病,导致压迫和侵犯下腔静脉和肺血栓栓塞的发展。这归因于下腔静脉的机械压迫和癌症的血栓形成作用。这是一种不寻常的精原细胞瘤的表现,在尸检中发现肺血栓栓塞后才做出诊断。
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引用次数: 0
Infanticide and neonaticide: How often are these victims concealed? 杀婴和杀新生儿:这些受害者经常被隐瞒吗?
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-15 DOI: 10.1111/1556-4029.70136
Gillian L. Johnson BSc, Edward B. Mondor PhD

Homicide rates in infants (under 1 year of age) and neonates (under 1 day of age) are greater than in any other childhood age group. Using data from the National Violent Death Reporting System (NVDRS) for the years 2018–2021, we sought to determine the demographics of infanticide and neonaticide perpetrators and victims, the incidence of both crimes, how often these victims were concealed, and the most common means of concealment. Here, we report that perpetrators of infanticide were predominantly male, while perpetrators of neonaticide were most often female. For infanticide, victims were most often male, whereas in neonaticide, there was no significant difference in the sex of the victims. Infanticides were far less likely to be concealed than neonaticides. When combining infanticides and neonaticides, we found that perpetrators were most often Black, aged 21–30, the parent, and also the caregiver at the time the crime was committed. Victims were most often concealed by being placed in bags or in the trash, respectively. By identifying which individuals are most likely to offend, intervention strategies can be developed for the highest risk populations. When these crimes cannot be prevented, knowing how the deaths are often concealed can be used to focus investigations to obtain justice for the young victims.

婴儿(1岁以下)和新生儿(1天以下)的凶杀率高于任何其他儿童年龄组。使用2018-2021年国家暴力死亡报告系统(NVDRS)的数据,我们试图确定杀婴和杀新生儿的肇事者和受害者的人口统计数据,这两种罪行的发生率,这些受害者被隐瞒的频率以及最常见的隐瞒手段。在这里,我们报告说,杀害婴儿的肇事者主要是男性,而杀害新生儿的肇事者通常是女性。在杀婴案中,受害者大多是男性,而在杀新生儿案中,受害者的性别没有显著差异。杀婴比杀新生儿更不容易被隐瞒。当把杀婴和杀新生儿结合起来时,我们发现犯罪者通常是黑人,年龄在21-30岁之间,是父母,也是犯罪发生时的照顾者。受害者通常被藏在袋子里或垃圾桶里。通过确定哪些人最有可能犯罪,可以针对最高风险人群制定干预策略。当这些罪行无法预防时,了解死亡往往是如何被隐瞒的,可以用来集中调查,为年轻的受害者伸张正义。
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引用次数: 0
“Hidden” dangers in a mass fatality aircraft crash incident: Heavy metal contamination in a forensic archaeological site in Northeast India 大规模致命飞机坠毁事件中的“隐藏”危险:印度东北部法医考古遗址中的重金属污染。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-13 DOI: 10.1111/1556-4029.70133
Aanchal Kashyap MS, Gargi Jani MS, William R. Belcher PhD, Bhoomika Patel PhD

Bone composition is affected during the lifetime of the organism by the nutrition of the individual, whereas postmortem bone composition is affected by the surrounding burial environment. Additionally, the presence of certain elements in a buried environment can affect the structural composition of bone. The accumulation of these elements can indicate various pollutants in the working environment, both past and present. Forensic archaeologists may be exposed to contaminants that include chemical as well as biological hazards. By reconstructing the land use history of this World War II-era aircraft crash site, it is possible to preliminarily identify specific occupational hazards. The aim of the current study is to identify and characterize fragmentary (non-human) bone specimens found in forensic archaeological site in northeast India. This research also examines the presence and distribution of heavy metals within these archaeological bone samples and, therefore, within the excavated work environment. The study utilizes a multidisciplinary approach combining archaeological, anthropological, radiographic, and elemental analysis techniques. Radiographic analysis indicated altered bone mineral density in four fragmented bone samples. Whereas, elemental analysis techniques viz.; energy dispersive x-ray fluorescence (EDXRF) and inductively coupled plasma mass spectrometry (ICP-MS) showed altered concentration and distribution of the heavy metals across all the specimens. Our results demonstrate the presence of heavy metals in the recovered non-human bone specimens, implying that the burial environment was affected by the hazardous pollutants and fuel, thereby posing a significant health and occupational hazard to the forensic archaeologists.

骨骼组成在生物体的一生中受到个体营养的影响,而死后骨骼组成则受到周围埋葬环境的影响。此外,埋藏环境中某些元素的存在会影响骨骼的结构组成。这些元素的积累可以表明过去和现在工作环境中的各种污染物。法医考古学家可能会接触到包括化学和生物危害在内的污染物。通过重建这个二战时期飞机失事地点的土地利用历史,可以初步确定具体的职业危害。当前研究的目的是鉴定和表征在印度东北部法医考古遗址发现的碎片(非人类)骨标本。这项研究还检查了这些考古骨骼样本中重金属的存在和分布,因此,在挖掘的工作环境中。该研究采用多学科方法,结合考古学、人类学、放射学和元素分析技术。放射学分析显示在四个破碎的骨样本中改变了骨矿物质密度。然而,元素分析技术,即;能量色散x射线荧光(EDXRF)和电感耦合等离子体质谱(ICP-MS)显示重金属在所有样品中的浓度和分布发生了变化。我们的研究结果表明,在回收的非人类骨骼标本中存在重金属,这意味着埋葬环境受到有害污染物和燃料的影响,从而对法医考古学家的健康和职业构成重大危害。
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引用次数: 0
Post-mortem cardiomegaly descriptor: Call for consistent criteria 死后心脏肿大描述:要求标准一致。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-11 DOI: 10.1111/1556-4029.70135
Mark W. Kroll PhD, FAIMBE, FACC, Dwayne A. Wolf MD, PhD, Klaus Witte MD, FACC, Hugh Calkins MD, FACC, Sebastian N. Kunz MD, Howard E. Williams PhD

Although the post-mortem descriptor of cardiomegaly is an important component of understanding a sudden death, there is no unified definition. A recent survey reported the usage of heart weight correction models of Molina or Kitzman, for example, or simple step cutoffs such as 350, 400, 450, or 500 g in common use. The goal of the present study was to determine how a diagnosis of cardiomegaly relates to these definitions and heart weight using a database of sudden deaths using 1071 autopsy reports from across the USA in which the heart weight and the presence (n = 373) or not (n = 698) of cardiomegaly were recorded. We found that medical examiners appear not to use corrections for body weight but instead rely on step weight cutoffs, predominantly of 350, 400, 450, and 500 g. The decedent's age, weight, ethnicity, and toxicology did not tend to influence a diagnosis of cardiomegaly. The term cardiomegaly is being used with increasing frequency with an average increase of 3.6% per year. Consistency in the post-mortem use of cardiomegaly is lacking.

虽然死后对心脏肥大的描述是理解猝死的一个重要组成部分,但并没有统一的定义。最近的一项调查报告了莫利纳或基茨曼的心脏重量校正模型的使用,例如,或简单的步骤截断,如350,400,450或500g在常用。本研究的目的是确定心脏肥大的诊断与这些定义和心脏重量之间的关系,使用猝死数据库,该数据库使用来自美国各地的1071例尸检报告,其中记录了心脏重量和心脏肥大的存在(n = 373)或不存在(n = 698)。我们发现,医学检查人员似乎不使用体重校正,而是依赖于步重临界值,主要是350、400、450和500克。死者的年龄、体重、种族和毒理学对心脏肥大的诊断没有影响。“心脏肥大”一词的使用频率越来越高,平均每年增加3.6%。在死后使用心脏扩张术的一致性是缺乏的。
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引用次数: 0
Evaluating novel and conventional cell-separation techniques for sexual assault investigations 评估新的和传统的细胞分离技术的性侵犯调查。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-11 DOI: 10.1111/1556-4029.70131
Janine Schulte PhD, Simon Egger MSc, Sarah Kron, Eva Scheurer MD, Iris Schulz PhD

Biological evidence from sexual assaults frequently includes few male cells (i.e., spermatozoa) and numerous female cells (i.e., epithelial cells). In practice, their genetic analysis typically involves separating the victim's cells from the perpetrator's sperm using conventional differential extraction or advanced cell enrichment/capturing techniques. A descriptive study on simulated sexual assault samples was carried out by the recruitment of 10 heterosexual, monogamous couples. Post-coital swabs were collected before and after consensual sexual intercourse, with a sampling period of up to 96 h, and subjected to analysis to detect, quantify, and genotype adhering sperm by three distinct cell-separation techniques: differential extraction, laser capture microdissection, and DEPArray™. Methods differed in sperm detection and genotyping efficacy, while foreign DNA was identifiable up to 96 h. Time since intercourse and individuals were statistically significant factors (p ≤ 0.05) on male DNA yields, while hygienic behavior was not. Prior sperm enrichment was pivotal for cell capture technologies to counteract the abundance of epithelial cells, achieved by a prior mild digestion step for laser microdissection. Evaluating the advantages and disadvantages of standard and advanced methods provided a novel, comprehensive understanding of their merits, postulating that modern applications can assist conventional ones in challenging crime samples.

性侵犯的生物学证据通常包括很少的男性细胞(即精子)和大量的女性细胞(即上皮细胞)。在实践中,他们的基因分析通常包括使用传统的差异提取或先进的细胞富集/捕获技术将受害者的细胞从肇事者的精子中分离出来。通过招募10对一夫一妻制的异性恋夫妇,对模拟性侵犯样本进行了描述性研究。在双方同意的性交之前和之后收集性交后拭子,采样周期长达96小时,并通过三种不同的细胞分离技术进行分析,以检测,量化和基因型粘附精子:差分提取,激光捕获显微解剖和DEPArray™。不同方法的精子检测和基因分型效果不同,外源DNA可识别至96 h。性交时间和个体对雄性DNA产率的影响有统计学意义(p≤0.05),而卫生行为对雄性DNA产率的影响无统计学意义。预先的精子富集是细胞捕获技术的关键,以抵消上皮细胞的丰度,通过预先温和的消化步骤实现激光显微解剖。评估标准和先进方法的优缺点提供了对其优点的新颖,全面的理解,假设现代应用可以协助传统方法挑战犯罪样本。
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引用次数: 0
Mycology and palynology: Preliminary results in a forensic experimental laboratory in Colombia, South America 真菌学和孢粉学:南美洲哥伦比亚法医实验室的初步结果。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-10 DOI: 10.1111/1556-4029.70116
María Cecilia Tranchida PhD, Leticia Karina Povilauskas PhD, Natalia Analía Ferreri PhD, Alejandra Baena PhD, Gretel González-Colmenares PhD, Maria Florencia Vianna PhD, Carlos Martín Molina PhD

Forensic mycology and palynology are emerging disciplines that offer valuable tools for solving crimes, particularly in cases of enforced disappearances and clandestine burials. This study, conducted in Bogotá, Colombia, aimed to explore the potential contributions of mycology and palynology in forensic investigations. Using a controlled experimental setup, we simulated clandestine graves and studied the fungal and pollen communities at different soil depths, aiming to identify decomposition markers and estimate burial times. Samples were taken from soil at 30 and 50 cm depths from both control graves and those with pig cadavers. Mycological analysis revealed differences in fungal diversity, with graves containing cadavers showing higher species richness, which is linked to cadaveric decomposition. Palynological analysis indicated that pollen grains in deeper soil layers (50 cm) correspond to species, such as Borago officinalis, Poa sp., and Croton sonderianus, linked to dry seasons, indicating the burial timeframe. On the other hand, pollen from 30 cm depth was consistent with species from the wet season, corroborating the exhumation period. This study highlights the utility of combining mycology and palynology in forensic investigations, especially in tropical or equatorial regions like Bogotá, where decomposition dynamics may differ significantly from other global contexts.

法医真菌学和孢粉学是新兴学科,为破案提供了宝贵的工具,特别是在强迫失踪和秘密埋葬案件中。本研究在哥伦比亚波哥大进行,旨在探讨真菌学和孢粉学在法医调查中的潜在贡献。利用控制实验装置,模拟地下坟墓,研究不同土壤深度下的真菌和花粉群落,旨在识别分解标记并估计埋葬时间。从对照坟墓和猪尸体坟墓的30和50厘米深度的土壤中采集样本。真菌学分析揭示了真菌多样性的差异,含有尸体的坟墓显示出更高的物种丰富度,这与尸体分解有关。孢粉学分析表明,较深土层(50 cm)的花粉粒对应的物种,如Borago officinalis、Poa sp.和Croton sonderianus,与干旱季节有关,表明埋埋时间。另一方面,30 cm深度的花粉与雨季的种类一致,证实了挖掘时间。这项研究强调了真菌学和孢粉学结合在法医调查中的效用,特别是在像波哥大这样的热带或赤道地区,那里的分解动力学可能与其他全球环境有很大不同。
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引用次数: 0
An alternate method for casting of three-dimensional footwear imprints in snow and sand 在雪和沙中铸造三维鞋印的另一种方法。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-09 DOI: 10.1111/1556-4029.70117
Hal Sherman BS, Peter Diaczuk PhD, Nicholas Petraco MS

Great Stuff™ is presented as an alternative material for the casting and preservation of three-dimensional footwear impressions located in snow and sand. This method generates a detailed, 1:1 foam cast of questioned footwear imprints. Great Stuff™ is a readily available commercial grade construction foam, normally used to fill voids and cracks in walls and interior window installations. The spray foam is applied by squirting the compound from the can into the footwear impression. A compression tool made from a wood plank fitted with two handles and covered on its base with a sheet of low-density polyethylene (LDPE) plastic is placed over the foam-filled impression. The wood plank is gently pushed downward onto the impression. After a short period, the foam cast hardens. The cast is then removed, cleaned, and trimmed. It is then placed into an evidence box and marked for I.D. The resulting questioned impression can be directly compared to the outsoles of known suspected items of footwear or impressions of known items of footwear made with Great Stuff™ foam.

Great Stuff™是一种替代材料,用于铸造和保存位于雪和沙子中的三维鞋类印痕。这种方法产生一个详细的,1:1泡沫模型的质疑鞋印。Great Stuff™是一种现成的商业级建筑泡沫,通常用于填充墙壁和室内窗户装置的空隙和裂缝。喷雾泡沫是通过将化合物从罐中喷射到鞋印中来应用的。一个由木板制成的压缩工具,配有两个手柄,并在其基础上覆盖一层低密度聚乙烯(LDPE)塑料,放置在泡沫填充的印象上。木板轻轻地向下推到印痕上。一段时间后,泡沫铸件变硬。然后取出铸件,清洗和修整。然后将其放入证据箱并标记身份。由此产生的可疑印痕可直接与已知可疑鞋类物品的外底或由Great Stuff™泡沫制成的已知鞋类物品的印痕进行比较。
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引用次数: 0
Quality issue management and disclosure in forensic science: A survey of practice and perceptions 法医学的质量问题管理和信息披露:实践和观念的调查。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-07-07 DOI: 10.1111/1556-4029.70121
Anna L. Heavey MBA, Max M. Houck PhD, Gavin R. Turbett PhD, Simon W. Lewis PhD

Addressing calls for transparency regarding errors and limitations in forensic processes is an ongoing concern for the forensic science service provider community and the stakeholders it serves worldwide. Foundational to this goal is developing a consistent approach to the identification of issues that have, or could have, an impact on the quality and reliability of forensic results. A standardized approach to the classification of quality issues detected within forensic agency management systems may be the strategic key to supporting consistent identification and disclosure, along with enhancing a positive quality culture throughout forensic service providers and building understanding of “error” in forensic science with end users of forensic information. A survey of international forensic science service providers was conducted to gain deeper insights into current systems of issue identification, classification, management, and disclosure along with perceptions on quality issues, their use and communication by forensic agency staff. The survey results demonstrate that development of a standardized approach would be of significant value to the forensic science community and its stakeholders, with potential benefit not only to improved communication and use of quality issue data but also in advancing a positive culture of quality and credibility in forensic service provision to support justice outcomes.

解决关于法医过程中错误和限制的透明度的呼吁是法医科学服务提供者社区及其在世界范围内所服务的利益攸关方持续关注的问题。实现这一目标的基础是制定一种一致的方法,以确定已经或可能对法医结果的质量和可靠性产生影响的问题。对法医机构管理系统中发现的质量问题进行标准化分类的方法可能是支持一致的鉴定和披露的战略关键,同时还可以在法医服务提供者中加强积极的质量文化,并与法医信息的最终用户建立对法医科学“错误”的理解。对国际法医科学服务提供商进行了一项调查,以更深入地了解当前的问题识别、分类、管理和披露系统,以及对质量问题的看法、它们的使用和法医机构工作人员的沟通。调查结果表明,标准化方法的发展对法医科学界及其利益相关者具有重要价值,不仅可以改善质量问题数据的沟通和使用,还可以促进法医服务提供质量和可信度的积极文化,以支持司法结果。
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引用次数: 0
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Journal of forensic sciences
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