With further development of generative AI, primarily generative-adversarial networks (GAN), deepfakes are gaining in quality and accessibility. While, forensic methods designed for examination of handwriting are often applied to its digital copies, despite being possibly insensitive to cases of GAN-made forgeries (unless methods of digital forensics are co-employed). Approaching this problem from a novel perspective, we have created a translational GAN tasked with generating false handwritten signatures from limited examples, aiming to ascertain whether traditional methods of signature examination will be effective against such forgeries. We have found that traditional methods of handwriting examination are sufficient for identification of discriminative features that could result in rejection of GAN-made forgeries, however, those stemmed mostly from the lesser visual quality of the generated signatures, which could be improved in the future.
{"title":"Generative-adversarial network for falsification of handwritten signatures","authors":"Maciej Marcinowski-Prażmowski PhD","doi":"10.1111/1556-4029.15680","DOIUrl":"10.1111/1556-4029.15680","url":null,"abstract":"<p>With further development of generative AI, primarily generative-adversarial networks (GAN), deepfakes are gaining in quality and accessibility. While, forensic methods designed for examination of handwriting are often applied to its digital copies, despite being possibly insensitive to cases of GAN-made forgeries (unless methods of digital forensics are co-employed). Approaching this problem from a novel perspective, we have created a translational GAN tasked with generating false handwritten signatures from limited examples, aiming to ascertain whether traditional methods of signature examination will be effective against such forgeries. We have found that traditional methods of handwriting examination are sufficient for identification of discriminative features that could result in rejection of GAN-made forgeries, however, those stemmed mostly from the lesser visual quality of the generated signatures, which could be improved in the future.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 2","pages":"770-778"},"PeriodicalIF":1.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Forensic entomology relies on known geographic ranges and seasonal presence of forensically relevant insects. In the Northeastern United States, there is no information on species in the region in early spring. Two forensic entomology workshops took place in April of 2023 and 2024 in Milton, Massachusetts. During this workshop, practitioners were trained in the appropriate collection and storage techniques prior to a practical experience including the collection of insects from pig and rabbit remains. All insects collected were identified down to family or species level. Across both years, Phormia regina (Meigen) (Diptera: Calliphoridae) was the fly consistently colonizing remains and was the oldest immature larvae in all instances. Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae), Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae), and Muscina stabulans (Fallén) (Diptera: Muscidae) were additional flies co-colonizing remains with P. regina. Other species were found as adults surrounding the remains that included other necrophagous fly species, beetles, and ants. Variation in insects collected between years likely due to high temperature variation. This documentation of species provides context to forensically related flies expected in Eastern Massachusetts and provides research directives in the region.
{"title":"Report of forensically relevant insects collected from pig and rabbit remains during two forensic entomology workshops in Eastern Massachusetts","authors":"Samantha J. Sawyer PhD","doi":"10.1111/1556-4029.15681","DOIUrl":"10.1111/1556-4029.15681","url":null,"abstract":"<p>Forensic entomology relies on known geographic ranges and seasonal presence of forensically relevant insects. In the Northeastern United States, there is no information on species in the region in early spring. Two forensic entomology workshops took place in April of 2023 and 2024 in Milton, Massachusetts. During this workshop, practitioners were trained in the appropriate collection and storage techniques prior to a practical experience including the collection of insects from pig and rabbit remains. All insects collected were identified down to family or species level. Across both years, <i>Phormia regina</i> (Meigen) (Diptera: Calliphoridae) was the fly consistently colonizing remains and was the oldest immature larvae in all instances. <i>Calliphora vicina</i> (Robineau-Desvoidy) (Diptera: Calliphoridae), <i>Protophormia terraenovae</i> (Robineau-Desvoidy) (Diptera: Calliphoridae), and <i>Muscina stabulans</i> (Fallén) (Diptera: Muscidae) were additional flies co-colonizing remains with <i>P. regina</i>. Other species were found as adults surrounding the remains that included other necrophagous fly species, beetles, and ants. Variation in insects collected between years likely due to high temperature variation. This documentation of species provides context to forensically related flies expected in Eastern Massachusetts and provides research directives in the region.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 2","pages":"763-769"},"PeriodicalIF":1.5,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The above article, first published online 31st October 2023, on Wiley Online Library (wileyonlinelibrary.com), has been retracted at the request of the authors, and by agreement between the Editor in Chief, Michael Peat PhD, the American Academy of Forensic Science, and John Wiley & Sons Inc. This retraction has been made because of the omission of the authorship of two co-investigators and premature submission of the manuscript that prevented corrections from, and approval by, the full research team. There was also a departure from the authorizing committee's terms for acknowledgment.
{"title":"Retraction: M. Ashton, N. Czado, M. Harrel, S. Hughes. “Genotyping strategies for tissues fixed with various embalming fluids for human identification, databasing, and traceability,” Journal of Forensic Sciences (Early View) https://doi.org/10.1111/1556-4029.15414.","authors":"","doi":"10.1111/1556-4029.15684","DOIUrl":"10.1111/1556-4029.15684","url":null,"abstract":"<p>The above article, first published online 31<sup>st</sup> October 2023, on Wiley Online Library (wileyonlinelibrary.com), has been retracted at the request of the authors, and by agreement between the Editor in Chief, Michael Peat PhD, the American Academy of Forensic Science, and John Wiley & Sons Inc. This retraction has been made because of the omission of the authorship of two co-investigators and premature submission of the manuscript that prevented corrections from, and approval by, the full research team. There was also a departure from the authorizing committee's terms for acknowledgment.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 1","pages":"410"},"PeriodicalIF":1.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15684","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kelly M. Elkins PhD, Shervonne Joseph BS, Cassandra Skrant MSFS
Training is an essential component of onboarding new hires in forensic science service provider (FSSP) laboratories. There are several DNA training standards published by the American Academy of Forensic Sciences (AAFS) Academy Standards Board (ASB) American National Standards Institute (ANSI) accredited framework. In this study, we conducted a survey of forensic DNA laboratory training programs to better understand training activities and materials. The survey was approved by the IRB and emailed to forensic laboratory directors, assistant directors, and/or DNA technical leaders and responses were submitted by them or their designees. Over thirty leaders and stakeholders responded. In this article, we report on the results of the survey. Respondents indicated that training activities included readings, writing assignments and quizzes, shadowing analysts, and mock casework and that training is documented and is a collaborative effort of the technical leader, unit supervisor, advanced forensic scientists, and other analysts and technicians. Laboratories assess competency using multiple methods including performance on mock casework, report writing, laboratory practical and competency tests, and a mock trial and testimony. The top three training activities reported are hands-on practice, shadowing, and readings. The top three focuses of the training are laboratory techniques (extraction, quantitation, amplification, and capillary electrophoresis), STR typing, and mixture analysis. Ethics violations and failure to pass the competency tests and mock trial, even after remediation, are the top reasons new hires fail training. Finally, the top items respondents would like to spend more time offering training on are troubleshooting, validation, and root cause analysis.
培训是法医学服务提供商(FSSP)实验室新员工入职培训的重要组成部分。美国法证科学院(AAFS)学院标准委员会(ASB)美国国家标准学会(ANSI)认可框架发布了多项DNA培训标准。在本研究中,我们对法医 DNA 实验室培训项目进行了调查,以更好地了解培训活动和材料。该调查经 IRB 批准,并通过电子邮件发送给法医实验室主任、助理主任和/或 DNA 技术负责人,由他们或其指定人员提交回复。三十多位领导和相关人员做出了回复。本文将报告调查结果。受访者表示,培训活动包括阅读、写作作业和测验、跟班分析师和模拟案件工作,培训有文件记录,是技术负责人、单位主管、高级法证科学家以及其他分析师和技术人员的共同努力。实验室采用多种方法评估能力,包括模拟办案、撰写报告、实验室实践和能力测试以及模拟审判和作证。据报告,排名前三位的培训活动是实际操作、跟班学习和阅读。培训的三大重点是实验室技术(提取、定量、扩增和毛细管电泳)、STR 分型和混合物分析。违反职业道德以及即使经过补救也无法通过能力测试和模拟审判是新员工培训失败的首要原因。最后,受访者希望花更多时间提供培训的首要项目是故障排除、验证和根本原因分析。
{"title":"Survey on forensic DNA biology training in forensic science service laboratories in the United States","authors":"Kelly M. Elkins PhD, Shervonne Joseph BS, Cassandra Skrant MSFS","doi":"10.1111/1556-4029.15671","DOIUrl":"10.1111/1556-4029.15671","url":null,"abstract":"<p>Training is an essential component of onboarding new hires in forensic science service provider (FSSP) laboratories. There are several DNA training standards published by the American Academy of Forensic Sciences (AAFS) Academy Standards Board (ASB) American National Standards Institute (ANSI) accredited framework. In this study, we conducted a survey of forensic DNA laboratory training programs to better understand training activities and materials. The survey was approved by the IRB and emailed to forensic laboratory directors, assistant directors, and/or DNA technical leaders and responses were submitted by them or their designees. Over thirty leaders and stakeholders responded. In this article, we report on the results of the survey. Respondents indicated that training activities included readings, writing assignments and quizzes, shadowing analysts, and mock casework and that training is documented and is a collaborative effort of the technical leader, unit supervisor, advanced forensic scientists, and other analysts and technicians. Laboratories assess competency using multiple methods including performance on mock casework, report writing, laboratory practical and competency tests, and a mock trial and testimony. The top three training activities reported are hands-on practice, shadowing, and readings. The top three focuses of the training are laboratory techniques (extraction, quantitation, amplification, and capillary electrophoresis), STR typing, and mixture analysis. Ethics violations and failure to pass the competency tests and mock trial, even after remediation, are the top reasons new hires fail training. Finally, the top items respondents would like to spend more time offering training on are troubleshooting, validation, and root cause analysis.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 1","pages":"61-72"},"PeriodicalIF":1.5,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eun-Mi Hwang MS, Kyu-Sik Jeong PhD, Seong Yeon Yoo PhD, Jihyun Kim PhD, Sanggil Choe PhD, Joo-Young Kim PhD
Lophophora williamsii, known for mescaline synthesis, has raised legal and ethical considerations. However, L. williamsii specimens that do not contain mescaline have been recently identified, necessitating the development of techniques to differentiate between mescaline-positive and mescaline-negative groups. Genetic markers have been explored to differentiate these specimens, complementing gas chromatography/mass spectrometry analysis. Here, we used a single-molecule real-time polymerase chain reaction to generate a full-length L. williamsii transcriptome and elucidate the mechanisms underlying mescaline biosynthesis. In total, 2,839,819 base pairs of highly accurate long reads were obtained using PacBio Iso-Seq and 70,945 unigenes were obtained through transcriptome sequencing. Known genes involved in the mescaline biosynthetic pathway were confirmed in the L. williamsii transcriptome, including 6 genes encoding tyrosine decarboxylases, 1 encoding tyrosine/DOPA decarboxylase, 215 encoding O-methyltransferases, and 129 encoding hydroxylases. Gene Ontology analysis revealed 2903 biological processes, 695 cellular components, and 1766 molecular functions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed two biosynthetic pathways, namely, phenylpropanoid and isoquinoline biosynthesis, associated with mescaline biosynthesis. To investigate genetic differences based on the presence of mescaline, Illumina Nova sequencing was conducted. The expression levels of genes involved in mescaline biosynthesis were compared to establish a candidate gene pool based on the presence of mescaline. Subsequently, functional annotation was performed on the identified candidate genes using a genetic database derived from PacBio sequencing. These findings underscore the need for a re-evaluation of forensic methods and legal regulations concerning newly identified L. williamsii specimens.
Lophophora williamsii 以合成麦司卡林而闻名,这引起了法律和伦理方面的考虑。不过,最近发现了不含麦司卡林的 L. williamsii 标本,因此有必要开发区分麦司卡林阳性和麦司卡林阴性群体的技术。基因标记被用来区分这些标本,作为气相色谱/质谱分析的补充。在这里,我们使用单分子实时聚合酶链反应生成了全长的 L. williamsii 转录组,并阐明了麦司卡林生物合成的内在机制。我们使用 PacBio Iso-Seq 获得了 2,839,819 个碱基对的高精度长读数,并通过转录组测序获得了 70,945 个单基因。在 L. williamsii 的转录组中确认了参与麦司卡林生物合成途径的已知基因,包括 6 个编码酪氨酸脱羧酶的基因、1 个编码酪氨酸/多巴胺脱羧酶的基因、215 个编码 O-甲基转移酶的基因和 129 个编码羟化酶的基因。基因本体分析显示了 2903 个生物过程、695 个细胞组分和 1766 个分子功能。京都基因和基因组百科全书(KEGG)通路分析显示,与麦司卡林生物合成相关的生物合成通路有两条,即苯丙类化合物和异喹啉的生物合成。为了研究麦司卡林存在的遗传差异,进行了 Illumina Nova 测序。通过比较参与麦司卡林生物合成的基因的表达水平,根据麦司卡林的存在情况建立了候选基因库。随后,利用 PacBio 测序得到的基因数据库对确定的候选基因进行了功能注释。这些发现突出表明,有必要重新评估有关新鉴定的 L. williamsii 标本的法医方法和法律规定。
{"title":"Comparative transcriptomic analysis provides novel insights into mescaline biosynthesis by Lophophora williamsii","authors":"Eun-Mi Hwang MS, Kyu-Sik Jeong PhD, Seong Yeon Yoo PhD, Jihyun Kim PhD, Sanggil Choe PhD, Joo-Young Kim PhD","doi":"10.1111/1556-4029.15679","DOIUrl":"10.1111/1556-4029.15679","url":null,"abstract":"<p><i>Lophophora williamsii</i>, known for mescaline synthesis, has raised legal and ethical considerations. However, <i>L. williamsii</i> specimens that do not contain mescaline have been recently identified, necessitating the development of techniques to differentiate between mescaline-positive and mescaline-negative groups. Genetic markers have been explored to differentiate these specimens, complementing gas chromatography/mass spectrometry analysis. Here, we used a single-molecule real-time polymerase chain reaction to generate a full-length <i>L. williamsii</i> transcriptome and elucidate the mechanisms underlying mescaline biosynthesis. In total, 2,839,819 base pairs of highly accurate long reads were obtained using PacBio Iso-Seq and 70,945 unigenes were obtained through transcriptome sequencing. Known genes involved in the mescaline biosynthetic pathway were confirmed in the <i>L. williamsii</i> transcriptome, including 6 genes encoding tyrosine decarboxylases, 1 encoding tyrosine/DOPA decarboxylase, 215 encoding O-methyltransferases, and 129 encoding hydroxylases. Gene Ontology analysis revealed 2903 biological processes, 695 cellular components, and 1766 molecular functions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed two biosynthetic pathways, namely, phenylpropanoid and isoquinoline biosynthesis, associated with mescaline biosynthesis. To investigate genetic differences based on the presence of mescaline, Illumina Nova sequencing was conducted. The expression levels of genes involved in mescaline biosynthesis were compared to establish a candidate gene pool based on the presence of mescaline. Subsequently, functional annotation was performed on the identified candidate genes using a genetic database derived from PacBio sequencing. These findings underscore the need for a re-evaluation of forensic methods and legal regulations concerning newly identified <i>L. williamsii</i> specimens.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 2","pages":"490-503"},"PeriodicalIF":1.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142735445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}