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Statistical analysis of fingerprint minutiae based on a large dataset and accurate minutiae detection method 基于大数据集的指纹细节统计分析和精确的细节检测方法。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-14 DOI: 10.1111/1556-4029.70216
Jiazhi Huang BEng, Yunqi Tang PhD, Kang Wang PhD, Yinxuan Qu MEng

Since the late 20th century, wrongful convictions based on fingerprint evidence and judicial scrutiny have raised questions about the scientific validity of fingerprint evidence, necessitating research into the scientific foundations of fingerprint identification. This article employs state-of-the-art AI algorithms to achieve fingerprint pattern classification, pose estimation, and minutiae detection. Based on a large-scale dataset of 620,211 fingerprint images, various analytical methods are applied to explore the relationships between fingerprint patterns, minutiae, and fingers, and statistical analysis is conducted on the quantity and spatial distribution of six types of minutiae: ridge endings, bifurcations, spurs, independent ridges, lakes, and crossovers. Compared with previous research, the average accuracy of minutiae detection is improved from 97.22% to 99.45%. Results indicate that whorls are the most common pattern, associated with thumbs and ring fingers, while loops are associated with middle and little fingers. The overall distribution ranges (in percentages) of the six types of minutiae are: ridge endings [58.288, 58.875], bifurcations [37.874, 38.421], spurs [1.301, 1.314], independent ridges [1.246, 1.260], lakes [0.415, 0.419], and crossovers [0.291, 0.295]. Spatial distribution analysis reveals that independent ridges exhibit concentrated distribution in delta regions, while the other types of minutiae are primarily concentrated in the core regions. This article quantifies the evidential value of different minutiae by analyzing the relationships among patterns, minutiae, and fingers as well as the spatial distribution of minutiae, providing a scientific statistical foundation for establishing probabilistic fingerprint identification models and contributing to improving objectivity and scientific rigor in fingerprint identification.

20世纪后期以来,基于指纹证据的错判和司法审查引发了对指纹证据科学有效性的质疑,需要对指纹识别的科学基础进行研究。本文采用最先进的人工智能算法来实现指纹模式分类、姿态估计和细节检测。基于620211张大规模指纹图像数据集,运用多种分析方法探讨指纹纹样、细部和手指之间的关系,对脊端、分岔、刺、独立脊、湖泊和交叉6种细部的数量和空间分布进行统计分析。与以往的研究相比,细微点检测的平均准确率由97.22%提高到99.45%。结果表明,环状纹是最常见的图案,与拇指和无名指有关,而环状纹与中指和小指有关。6类细部的总体分布范围(以百分比计)为:脊端[58.288、58.875]、分叉[37.874、38.421]、刺[1.301、1.314]、独立脊[1.246、1.260]、湖泊[0.415、0.419]、交叉[0.291、0.295]。空间分布分析表明,独立隆起主要集中分布在三角洲地区,其他类型的细碎主要集中在核心区。本文通过分析图案、细枝末节和手指之间的关系以及细枝末节的空间分布,量化了不同细枝末节的证据价值,为建立概率指纹识别模型提供了科学的统计基础,有助于提高指纹识别的客观性和科学性。
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引用次数: 0
DNA transfer in packaging: Investigation of mitigation strategies 包装中的DNA转移:缓解策略的调查。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-14 DOI: 10.1111/1556-4029.70217
Carl J. Stella BSc (Hons), Mariya Goray PhD, Georgina E. Meakin PhD, Roland A. H. van Oorschot PhD

Crime scene exhibits are often packaged at a crime scene and transported to a laboratory for DNA analysis. DNA-containing material may be lost from the sampling site of the exhibit to the inside of the packaging, preventing identification of a suspect, or may transfer to other parts of the exhibit complicating the interpretation of results. We sought to mitigate this DNA transfer by testing packaging that reduced direct contact with the exhibit, limited the exhibit's movement, or contained physical barriers to separate areas of the exhibit. Blood, saliva, or touch DNA were deposited onto mock exhibits that were packaged by one of four methods: unsecured, secured to bottom, secured suspended, or secured suspended with barrier separating areas. Packaged exhibits were then transported in a manner resembling casework, after which the location and amount of DNA on the exhibit and packaging were assessed. Control samples, which were not transported, were also tested. Touch and saliva deposits appeared to transfer by direct contact with the packaging and this transfer could be mitigated by suspending and/or securing the exhibits within packaging to minimize contact. Blood flaking from the exhibits meant the transfer of blood was inevitable under the conditions tested. While limiting direct contact between the exhibit and packaging minimized relocation of blood on the exhibit, the use of physical barriers prevented its transfer to other parts of the packaging. We show that while DNA transfer in packaging is not uncommon, there are strategies to mitigate this.

犯罪现场的证物通常在犯罪现场包装好,然后送到实验室进行DNA分析。含有dna的材料可能会从展品的采样地点丢失到包装内部,从而无法识别嫌疑人,或者可能转移到展品的其他部分,使结果的解释复杂化。我们试图通过测试包装来减少与展品的直接接触,限制展品的移动,或在展品的单独区域设置物理屏障来减轻这种DNA转移。血液、唾液或触摸DNA被沉积在模拟展品上,这些模拟展品被四种方法中的一种包装:不固定、固定到底部、固定悬挂或用屏障分隔区域固定悬挂。然后以类似于案件工作的方式运输包装的展品,之后评估展品和包装上DNA的位置和数量。未运输的对照样本也进行了检测。接触和唾液沉积物似乎通过与包装的直接接触而转移,可以通过将展品悬挂和/或固定在包装内以减少接触来减轻这种转移。展品上的血液剥落意味着在测试条件下血液的转移是不可避免的。虽然限制展品和包装之间的直接接触可以最大限度地减少展品上血液的迁移,但使用物理屏障可以防止血液转移到包装的其他部分。我们表明,虽然包装中的DNA转移并不罕见,但有一些策略可以减轻这种情况。
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引用次数: 0
Statistical analysis of fingerprint first-level detail using Bayesian networks 基于贝叶斯网络的指纹一级细节统计分析。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-10 DOI: 10.1111/1556-4029.70215
Keith B. Morris PhD, Jamie S. Spaulding PhD

This study presented a large-scale statistical examination of 168,974 tenprint records to evaluate whether pattern distribution across the fingers is random or exhibits structured interdependence, and whether sex-related differences in pattern frequency exist. Two Bayesian networks were empirically developed and validated to model the relationships between the pattern types of different fingers. The first network focused specifically on the occurrence of whorls and was evaluated relative to established frequencies of the Henry primary classification system, revealing expected relationships between pattern types, but also extending beyond, traditional classification approaches. The second network incorporated all major fingerprint pattern types to model probabilistic dependencies across fingers and hands. This work demonstrates and models significant inter- and intrahand relationships. Additionally, the developed Bayesian networks enable automated biometric identification system users to input their data to model finger variation for the computation of statistical conclusions. These relationships can be leveraged to predict pattern occurrences on other fingers which can be used to limit file penetration by filtering searches by finger position yielding increased search accuracy through a reduced search gallery.

本研究对168,974份手印记录进行了大规模的统计检查,以评估手指上的图案分布是随机的还是表现出结构性的相互依赖,以及图案频率是否存在与性别相关的差异。两个贝叶斯网络的经验开发和验证,以模拟不同手指模式类型之间的关系。第一个网络专门关注螺旋的发生,并相对于亨利主要分类系统的既定频率进行评估,揭示了模式类型之间的预期关系,但也超出了传统的分类方法。第二个网络结合了所有主要的指纹模式类型来模拟手指和手之间的概率依赖关系。这项工作证明并模拟了重要的内部和内部关系。此外,开发的贝叶斯网络使自动生物识别系统用户能够输入他们的数据来模拟手指变化,以计算统计结论。可以利用这些关系来预测其他手指上出现的模式,这可以通过按手指位置过滤搜索来限制文件渗透,从而通过减少搜索库来提高搜索精度。
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引用次数: 0
A study on quantifying the individuality of fingerprints and the 3D feature distribution of minutiae 指纹个性量化及细节三维特征分布研究。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-06 DOI: 10.1111/1556-4029.70214
Yaqi Yang MSc, Ruobing Qu BSc, Zhong Sun BSc, Bing Li PhD, Chunsheng Wu PhD, Dong Zhao PhD

The hypothesis of fingerprint individuality continues to be debated due to limited empirical verification, impacting the scientific foundation of fingerprint identification. This study proposed a quantitative model for fingerprint individuality and investigated the three-dimensional (3D) distribution of minutiae. This model considered the position and direction of minutiae as 3D feature variables. We extracted 3D feature data from 56,812,114 known fingerprints based on the automatic fingerprint identification system (AFIS). Following data calibration, translation, and error correction, we statistically analyzed the distribution density of minutiae. We developed the algorithm to calculate the individuality score of a single fingerprint through the individuality model. The experimental results showed that the minutiae distribution followed distinct patterns. The distribution density of minutiae exhibited symmetry between corresponding fingers on left/right hands. Significant variations in minutiae distribution density and central point distribution were observed across the five pattern types (whorl, left loop, right loop, arch, accidental). Minutiae with different directions exhibited symmetry along the Y-axis in both positional and quantitative distribution. Minutiae within diagonally opposite angular ranges showed similar distribution trends. The individuality scores were robust to distinguish different fingerprints. We preliminarily applied the individuality score to provide a basis for modifying the AFIS scoring mechanism, and we found that the individuality score of same-source fingerprints was greater than that of close nonmatches (CNMs). This work provides novel insights into fingerprint individuality and establishes a statistical foundation for refining AFIS scoring mechanisms and likelihood-ratio evidence evaluation frameworks.

由于经验验证的不足,指纹个性化假说一直存在争议,影响了指纹识别的科学基础。本研究提出了指纹个性的定量模型,并对指纹细节的三维分布进行了研究。该模型将细部的位置和方向作为三维特征变量。基于自动指纹识别系统(AFIS),提取了56,812,114个已知指纹的三维特征数据。经过数据校准、平移和误差校正,我们统计分析了细部的分布密度。我们开发了通过个性模型计算单个指纹个性分数的算法。实验结果表明,细部分布具有明显的规律。细部分布密度在左手/右手对应手指间呈现对称性。5种类型(螺旋型、左环型、右环型、拱形型和偶发型)的细部分布密度和中心点分布均存在显著差异。不同方向的细部在位置分布和数量分布上均沿y轴呈现对称性。对角线相反角度范围内的细部分布趋势相似。个性评分对不同指纹的区分具有较强的稳健性。我们初步应用个性评分为修改AFIS评分机制提供了依据,发现同源指纹的个性评分高于接近不匹配指纹的个性评分。这项工作提供了对指纹个性的新见解,并为完善AFIS评分机制和似然比证据评估框架奠定了统计基础。
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引用次数: 0
Enhancing malware detection and classification in network traffic using deep learning techniques 利用深度学习技术增强网络流量中的恶意软件检测和分类。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-06 DOI: 10.1111/1556-4029.70189
Pratibha Amol Tambewagh PhD, Dayanand Ingle PhD

Malware detection and classification in network traffic is a critical challenge in cybersecurity, with evolving threats that traditional methods struggle to address. As network traffic becomes more complex, accurately identifying malicious activities while minimizing false positives is essential for real-time monitoring systems. This study aims to enhance malware detection using deep learning (DL) techniques, focusing on improving accuracy, reducing false positives, and enabling real-time detection in dynamic network environments. Several advanced DL techniques are introduced to address these challenges. Entropy-Based Traffic Filtering (ETF) measures the randomness in network traffic to identify anomalies and malicious patterns, reducing noise and improving feature extraction. Self-Supervised Learning for Anomaly Detection (SSLAD) detects malware without labeled data by learning normal traffic patterns and identifying anomalies, thus improving the detection of unknown threats. Graph Neural Networks for Malware Traffic Classification (GNN-MTC) model network traffic as graphs, where devices are nodes, and communications are edges, capturing relational dependencies and anomalies to detect complex attack patterns like botnets and command-and-control (C2) communications. Context-Aware Graph Attention Networks (CA-GAT) further enhance detection by analyzing traffic as graphs while incorporating contextual factors like time and behavior, focusing on relevant interactions to improve attack detection. The proposed DL model achieves 98% accuracy, surpassing DeepMAL (95%) and an entropy-based method by Huang et al. (97.3%). Its strong precision and recall demonstrate superior performance in detecting known and novel malware, making it well-suited for real-time network security applications. The model was implemented using Python. Future research could focus on integrating real-time adaptive learning models, exploring hybrid DL architectures, and enhancing cross-platform malware detection, ensuring scalability and robustness in evolving network security environments.

网络流量中的恶意软件检测和分类是网络安全中的一个关键挑战,传统方法难以解决不断发展的威胁。随着网络流量变得越来越复杂,准确识别恶意活动,同时最大限度地减少误报对于实时监控系统至关重要。本研究旨在利用深度学习(DL)技术增强恶意软件检测,重点是提高准确性,减少误报,并在动态网络环境中实现实时检测。介绍了几种先进的深度学习技术来解决这些挑战。基于熵的流量过滤(ETF)衡量网络流量的随机性,以识别异常和恶意模式,降低噪声并改进特征提取。自监督学习异常检测(Self-Supervised Learning for Anomaly Detection, SSLAD)通过学习正常的流量模式和识别异常,在没有标记数据的情况下检测恶意软件,从而提高对未知威胁的检测能力。用于恶意软件流量分类的图形神经网络(GNN-MTC)将网络流量建模为图形,其中设备是节点,通信是边缘,捕获关系依赖性和异常,以检测复杂的攻击模式,如僵尸网络和命令与控制(C2)通信。上下文感知图注意网络(CA-GAT)通过将流量分析为图形进一步增强检测,同时结合时间和行为等上下文因素,关注相关交互以改进攻击检测。提出的深度学习模型达到98%的准确率,超过了DeepMAL(95%)和Huang等人基于熵的方法(97.3%)。其强大的精确度和召回率在检测已知和新型恶意软件方面表现出卓越的性能,使其非常适合实时网络安全应用。该模型是使用Python实现的。未来的研究可以集中在集成实时自适应学习模型,探索混合深度学习架构,增强跨平台恶意软件检测,确保在不断发展的网络安全环境中的可扩展性和鲁棒性。
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引用次数: 0
Beyond individual resilience: Organizational determinants of mental health outcomes for forensic scientists 超越个人弹性:法医科学家心理健康结果的组织决定因素。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-06 DOI: 10.1111/1556-4029.70220
Caitlin Rogers EdD
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引用次数: 0
Forensic detection of medical image manipulation using PACS and DICOM artifacts 使用PACS和DICOM伪影的医学图像处理的法医检测。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-11-02 DOI: 10.1111/1556-4029.70191
Sojung Oh MS, Eunji Lee MS, Seoyeon Lee MS, Jaehyun Park MS, Sihyun Park MS, Gibum Kim PhD

With the digitization of medical information, illegal activities such as medical crimes and insurance fraud through tampering have increased. Medical images are particularly vulnerable due to their nature as soft copies and their transmission over networks. National research institutions such as NIST provide guidelines that define security control elements for managing medical images, primarily out of concern for system vulnerabilities. However, there is still a lack of established or standardized digital forensic methodologies specifically tailored to the medical imaging domain. This study proposes a digital forensic technique for detecting manipulation in medical images. Two widely adopted PACS (Picture Archiving and Communication System) platforms were selected, and a dataset comprising 82 samples across 40 types of tampering scenarios was constructed. Tampering behaviors such as the editing or deletion of DICOM files were categorized, and forensic analysis of DICOM tags and system artifacts enabled identification of the type and origin of changes. An automated detection module was developed and tested on 110 validation cases. The results demonstrated accurate detection in all instances, depending on whether the changes were reflected in the actual DICOM files. This research marks the first digital forensic approach to medical image tampering detection and is expected to serve as a foundation for future investigative techniques in response to medical-related crimes.

随着医疗信息的数字化,医疗犯罪、篡改保险欺诈等违法行为有所增加。医学图像由于其软拷贝的性质和通过网络传输的特性而特别容易受到攻击。NIST等国家研究机构主要出于对系统漏洞的考虑,提供了定义用于管理医学图像的安全控制元素的指导方针。然而,目前仍然缺乏专门针对医学成像领域的既定或标准化的数字法医方法。本研究提出一种用于检测医学图像操纵的数字法医技术。选取了两种被广泛采用的PACS(图片存档和通信系统)平台,构建了包含40种篡改场景的82个样本的数据集。诸如编辑或删除DICOM文件之类的篡改行为被分类,并且DICOM标记和系统工件的取证分析能够识别更改的类型和来源。开发了自动检测模块,并对110个验证用例进行了测试。结果显示在所有实例中都能准确检测到,这取决于更改是否反映在实际的DICOM文件中。这项研究标志着医学图像篡改检测的第一个数字法医方法,并有望成为未来应对医疗相关犯罪的调查技术的基础。
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引用次数: 0
1, 2, 3 crimes you're out: Ocular-motor methods for detecting deception in a multiple-issue screening protocol 一,二,三次犯罪你出局了:在多问题筛选协议中,用眼动法检测欺骗。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-31 DOI: 10.1111/1556-4029.70209
Andrew C. Potts PhD, Andrea K. Webb PhD

The ocular-motor deception test (ODT) uses pupil dilations and reading behaviors to detect deception. It questions examinees on two illicit activities but classifies deception for only one. Previous studies demonstrate that the ODT discriminates between truthful and deceptive individuals with over 80% accuracy. The present study considered a four-topic screening test and evaluated whether ocular-motor measures could discriminate between truthful and deceptive individuals and pinpoint the specific topic(s) answered deceptively. We recruited 180 participants from the community and the University of Utah. Sixty participants stole $20 (cash), 60 participants stole $20 and a VISA gift card (cash + card), and 60 participants were innocent (innocent). We asked participants about their involvement in four mock crimes: theft of $20, theft of a VISA gift card, vandalism of a parking kiosk, and filing a false police report. Cash participants were deceptive regarding cash statements, cash + card participants were deceptive regarding cash and card statements, and innocent participants were truthful regarding all statements. The computer compared reactions to cash, card, and vandalism statements to those on false report statements to detect deception. As predicted, cash and cash + card participants showed significant changes in ocular-motor measures to cash and both cash and card statements, respectively. A logistic regression model correctly classified 83.3% of innocent participants, 91.7% of cash participants, and 85.5% of cash + card participants. The model correctly classified 86.1%, 81.1%, and 88.3% of answers to the cash, VISA card, and vandalism items, respectively. The findings suggest that a multiple-issue ODT could be valuable in screening applications.

眼动欺骗测试(ODT)利用瞳孔扩张和阅读行为来检测欺骗。它向考生提问两项违法行为,但只将其中一项列为欺诈行为。先前的研究表明,ODT区分诚实和欺骗个体的准确率超过80%。本研究考虑了一个四主题筛选测试,并评估了眼运动测量是否可以区分诚实和欺骗的个体,并确定欺骗性回答的特定主题。我们从社区和犹他大学招募了180名参与者。60名参与者偷了20美元(现金),60名参与者偷了20美元和一张VISA礼品卡(现金+卡),60名参与者无罪(无辜)。我们向参与者询问了他们在四种模拟犯罪中的参与情况:盗窃20美元,盗窃VISA礼品卡,破坏停车亭,以及向警方提交虚假报告。现金参与者在现金报表上具有欺骗性,现金+卡参与者在现金和卡报表上具有欺骗性,而无辜的参与者在所有报表上都是真实的。计算机将人们对现金、信用卡和破坏公物陈述的反应与对虚假报告陈述的反应进行比较,以检测欺骗行为。正如预测的那样,现金和现金+卡的参与者分别在现金和现金和卡对账单的眼动测量中表现出显著的变化。逻辑回归模型正确分类了83.3%的无辜参与者、91.7%的现金参与者和85.5%的现金+卡参与者。该模型对现金、VISA卡和破坏行为的答案分别正确分类了86.1%、81.1%和88.3%。研究结果表明,多问题ODT可能在筛选应用中有价值。
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引用次数: 0
A behavioral health needs assessment and general psychological well-being of digital and multimedia forensic examiners 数字和多媒体法医审查员的行为健康需求评估和一般心理健康。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-31 DOI: 10.1111/1556-4029.70207
Sonali Tyagi MS, Kathryn C. Seigfried-Spellar PhD

Research shows that digital forensic examiners experience high stress levels due to the nature of their jobs involving exposure to disturbing media. This study conducted a needs analysis by examining digital and multimedia forensic examiners' psychological well-being, coping mechanisms, social support, and attitudes toward and experiences with barriers to counseling and mental health support. Ninety-four digital and multimedia forensic examiners (DFE) completed the anonymous online survey. Respondents were also asked to self-report their primary duty within digital forensics (e.g., image, audio, or video analysts) and whether they were also working as an investigator/detective; 53 were DFE-only, and 41 had dual roles (DFE + detective). Results examined differences in primary duties (e.g., image vs. non-image analyst) and the number of primary duties (e.g., two vs. three). Of the sample, 36% personally sought counseling due to work-related stress. Image forensic analysts reported more psychological distress and barriers toward help-seeking compared with audio and video analysts. 17% (n = 16) of the sample met the diagnostic criteria for PTSD. There were no significant differences between DFE-only and those working dual roles as detectives on psychological well-being and attitudes toward mental health support. Finally, digital forensic examiners who met the diagnostic criteria for PTSD reported 9 out of 15 mental health stigmas, many of which included fear associated with agency culture (e.g., “affect my promotion”). Findings support the need for accessible, agency-supported, and potentially mandated mental health services for DFE to improve well-being and resiliency.

研究表明,由于他们的工作性质涉及接触令人不安的媒体,数字法医审查员承受着很高的压力。本研究透过调查数位及多媒体法医的心理健康状况、应对机制、社会支持、心理咨询及心理健康支持障碍的态度及经验,进行需求分析。94名数字和多媒体法医(DFE)完成了匿名在线调查。受访者还被要求自我报告他们在数字取证中的主要职责(例如,图像、音频或视频分析师),以及他们是否同时担任调查员/侦探;53只DFE, 41双角色(DFE +侦探)。结果检查了主要职责(例如,图像与非图像分析师)和主要职责的数量(例如,两个与三个)的差异。在样本中,36%的人因工作压力而亲自寻求咨询。与音频和视频分析师相比,图像法医分析师报告了更多的心理困扰和寻求帮助的障碍。17% (n = 16)的样本符合PTSD的诊断标准。在心理健康状况和心理健康支持态度方面,双职侦探与单职侦探在心理健康状况和心理健康支持态度方面无显著差异。最后,符合创伤后应激障碍诊断标准的数字法医审查员报告了15种心理健康耻辱中的9种,其中许多包括与机构文化相关的恐惧(例如,“影响我的晋升”)。调查结果表明,有必要为DFE提供可获得的、机构支持的和潜在的强制性心理健康服务,以提高福祉和弹性。
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引用次数: 0
Impact of flunitrazepam on Calliphora vicina (Diptera: Calliphoridae) microbiome dynamics 氟硝西泮对姬蝇(双翅目:姬蝇科)微生物群动力学的影响。
IF 1.8 4区 医学 Q2 MEDICINE, LEGAL Pub Date : 2025-10-30 DOI: 10.1111/1556-4029.70208
Lavinia Iancu PhD, Ranjana Mosby BS, Andrea Bonicelli PhD, Noemi Procopio PhD

This study explored the effects of flunitrazepam, a benzodiazepine, on the microbiome diversity of Calliphora vicina Robineau-Desvoidy, 1830 (Diptera: Calliphoridae). By examining the microbial shifts throughout developmental stages, the research contributes valuable data to the field of forensic entomotoxicology. Two colonies of 500 adults were fed minced beef liver, spiked and non-spiked with 25 mg of flunitrazepam, and reared under controlled conditions (24°C, relative humidity of 45%, 12:12 light–dark cycle). Following oviposition, egg clusters were transferred, and the experiment was carried out in triplicate under the same experimental conditions. A total of 54 specimens, including all developmental stages, were collected for microbiome investigation via Illumina MiSeq. Both colonies had a 19-day development cycle from eggs to teneral. However, flunitrazepam-fed specimens were heavier, particularly during the pupa and teneral stages. Microbiome analysis revealed significant differences in diversity and composition between the colonies and across developmental stages. Pseudomonadota (Proteobacteria) dominated the control adults, while Bacteroidota and Bacillota (Firmicutes) were more prevalent in flunitrazepam-fed adults. Additionally, Enterobacterales, Lactobacillales, and Morganellaceae showed notable variations across different stages. This study highlights the significant impact of flunitrazepam on the microbiome dynamics of C. vicina, revealing notable morphological changes related to the specimens' weight toward the end of the development cycle and alterations in microbiome composition. These findings have important implications for forensic entomotoxicology, particularly in the accurate estimation of the minimum postmortem interval (mPMI).

本研究探讨了氟硝西泮(一种苯二氮卓类药物)对小蝇蛆(Calliphora vicina Robineau-Desvoidy, 1830)微生物群多样性的影响。通过检查微生物在整个发育阶段的变化,该研究为法医昆虫毒理学领域提供了有价值的数据。两群500只成虫分别饲喂牛肝碎,添加氟硝西泮和未添加氟硝西泮各25 mg,饲养在受控条件下(24°C,相对湿度45%,12:12明暗循环)。产卵后,转移卵簇,在相同的实验条件下进行三次实验。采用Illumina MiSeq软件对54份标本进行微生物组学分析,包括所有发育阶段。两个群体从卵到蛹的发育周期均为19天。然而,氟硝西泮喂养的标本更重,特别是在蛹期和幼虫期。微生物组分析显示,菌落之间和不同发育阶段的微生物组多样性和组成存在显著差异。对照组以假单胞菌(变形菌门)为主,氟硝西泮组以拟杆菌门和杆菌门(厚壁菌门)为主。此外,肠杆菌、乳酸杆菌和摩根菌科在不同阶段表现出显著的差异。本研究强调了氟硝西泮对C. vicina微生物组动力学的显著影响,揭示了发育周期结束时与标本体重相关的显著形态学变化和微生物组组成的变化。这些发现对法医昆虫毒理学具有重要意义,特别是在准确估计最小死后间隔(mPMI)方面。
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Journal of forensic sciences
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