Hrvoje Senješ MSc, Sunčica Kuzmić MSc, Ivan Jerković PhD
Inorganic gunshot residue (iGSR) analysis, crucial for linking suspects to firearm use, faces challenges from potential environmental contamination, notably in police vehicles. The present study aimed to explore the level of iGSR contamination in police vehicles from the Zagreb County Police Administration (Croatia), considering particle types and their position in vehicles, and to identify associated risk factors. From December 2021 to April 2022, 65 of 86 police vehicles (margin of error: ±6% at a 95% confidence level) were sampled with GSR stubs on the drivers' seats, back seats, and backrests and analyzed using scanning electron microscopy and energy dispersive x-ray analysis (SEM/EDX). Characteristic particles were found in 63.1% of vehicles, 33.8% on the driver's seat, and 24.6% on the back seat/backrest. Indicative particles were found in 70.77% of vehicles, with a fairly even distribution. McNemar's chi-square analysis showed no significant disparities in positive sample ratios across vehicle parts or particle types (p > 0.05). In total, 228 characteristic and 166 indicative GSR particles were identified, with no notable correlation among them (p = 0.346). Logistic regression analysis identified the transportation of individuals involved in firearms incidents as a statistically significant factor influencing the presence of characteristic particles (p = 0.030). The findings suggest a considerable prevalence of iGSR in the analyzed Police Administration unit, highlighting the need for careful contamination management in police operations to preserve evidence integrity, particularly in cases when individuals who used firearms had been transported in the vehicle.
{"title":"Police vehicle contamination by inorganic gunshot residue (iGSR) in Zagreb County Police Administration (Croatia): Analysis of characteristic and indicative particles across different vehicle parts and contributory risk factors","authors":"Hrvoje Senješ MSc, Sunčica Kuzmić MSc, Ivan Jerković PhD","doi":"10.1111/1556-4029.15628","DOIUrl":"10.1111/1556-4029.15628","url":null,"abstract":"<p>Inorganic gunshot residue (iGSR) analysis, crucial for linking suspects to firearm use, faces challenges from potential environmental contamination, notably in police vehicles. The present study aimed to explore the level of iGSR contamination in police vehicles from the Zagreb County Police Administration (Croatia), considering particle types and their position in vehicles, and to identify associated risk factors. From December 2021 to April 2022, 65 of 86 police vehicles (margin of error: ±6% at a 95% confidence level) were sampled with GSR stubs on the drivers' seats, back seats, and backrests and analyzed using scanning electron microscopy and energy dispersive x-ray analysis (SEM/EDX). Characteristic particles were found in 63.1% of vehicles, 33.8% on the driver's seat, and 24.6% on the back seat/backrest. Indicative particles were found in 70.77% of vehicles, with a fairly even distribution. McNemar's chi-square analysis showed no significant disparities in positive sample ratios across vehicle parts or particle types (<i>p</i> > 0.05). In total, 228 characteristic and 166 indicative GSR particles were identified, with no notable correlation among them (<i>p</i> = 0.346). Logistic regression analysis identified the transportation of individuals involved in firearms incidents as a statistically significant factor influencing the presence of characteristic particles (<i>p</i> = 0.030). The findings suggest a considerable prevalence of iGSR in the analyzed Police Administration unit, highlighting the need for careful contamination management in police operations to preserve evidence integrity, particularly in cases when individuals who used firearms had been transported in the vehicle.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2230-2239"},"PeriodicalIF":1.5,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ragy R. Girgis MD, Dung Hoang MA, Hannah Hesson BA, Gabriella Dishy MA, Kathryn Lee MA, Tyler Pia MA, Faizan Syed MD, Alexandra Villalobos MA, Paul S. Appelbaum MD, Gary Brucato PhD
Most research on mass murderers to date has focused on perpetrators of male sex, while research on perpetrators of female sex has been relegated to case reports and series. We aimed to more fully examine the phenomenon of female-perpetrated mass murder. We analyzed 1715 worldwide incidents of personal-cause mass murder from 1900 to 2019, identifying 105 (6%) events perpetrated by females. We defined mass murder as any event involving at least three fatalities, not including the perpetrator, using any method. We identified cases of mass murder from English-language databases of mass murder or murder in print or online. There were no significant differences in age and race between female and male perpetrators. Relative to males, female perpetrators were significantly less likely to employ firearms in their mass murders, using them in less than half of cases, compared to over 70% for males. The prevalence of psychotic signs and symptoms among female mass murderers was more than double that among males (25.7% vs. 12.5%, p < 0.01), while the rate of nonpsychotic psychiatric or neurological conditions was also much greater among female perpetrators (29.5% vs. 17.1%, p < 0.01). Over half of female perpetrators took or attempted to take their own lives. More than three-quarters of mass murders by females involved at least one family member as a victim. This study underscores sex-specific differences in the perpetration of mass murder and the need for further research to understand how insights about such dynamics might lead to the development of more effective and informed intervention policies.
{"title":"An analysis of 105 female-perpetrated mass murders","authors":"Ragy R. Girgis MD, Dung Hoang MA, Hannah Hesson BA, Gabriella Dishy MA, Kathryn Lee MA, Tyler Pia MA, Faizan Syed MD, Alexandra Villalobos MA, Paul S. Appelbaum MD, Gary Brucato PhD","doi":"10.1111/1556-4029.15627","DOIUrl":"10.1111/1556-4029.15627","url":null,"abstract":"<p>Most research on mass murderers to date has focused on perpetrators of male sex, while research on perpetrators of female sex has been relegated to case reports and series. We aimed to more fully examine the phenomenon of female-perpetrated mass murder. We analyzed 1715 worldwide incidents of personal-cause mass murder from 1900 to 2019, identifying 105 (6%) events perpetrated by females. We defined mass murder as any event involving at least three fatalities, not including the perpetrator, using any method. We identified cases of mass murder from English-language databases of mass murder or murder in print or online. There were no significant differences in age and race between female and male perpetrators. Relative to males, female perpetrators were significantly less likely to employ firearms in their mass murders, using them in less than half of cases, compared to over 70% for males. The prevalence of psychotic signs and symptoms among female mass murderers was more than double that among males (25.7% vs. 12.5%, <i>p</i> < 0.01), while the rate of nonpsychotic psychiatric or neurological conditions was also much greater among female perpetrators (29.5% vs. 17.1%, <i>p</i> < 0.01). Over half of female perpetrators took or attempted to take their own lives. More than three-quarters of mass murders by females involved at least one family member as a victim. This study underscores sex-specific differences in the perpetration of mass murder and the need for further research to understand how insights about such dynamics might lead to the development of more effective and informed intervention policies.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2120-2126"},"PeriodicalIF":1.5,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142217126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hojeong Seo, Saehan Park, Sanghee Kim, Sookyoung Lee, Changho Choi
This study investigates the cardiac safety concerns related to TASER discharges centering on a pivotal case that marked the first TASER‐related fatality in South Korea. Employing Pratt et al.'s theoretical framework, the research evaluates the potential for ventricular fibrillation (VF) from these discharges. The methodology incorporated a high‐resolution waveform analysis using sophisticated equipment and considered specific incident details, including dart impact locations verified through a forensic examination. A human body impedance of 500 Ω, chosen based on empirical studies and coupled with non‐inductive resistance for high‐voltage handling, was utilized in the model. By applying a heart‐current factor from IEC 60479 standards, the study found a VF risk of up to 5% depending on the impact location and current pathways. In this specific case, although the calculated risk did not exceed critical thresholds, the VF risk was high enough to suggest that TASER discharges played a role in the fatal outcome. This study underscores the importance of dart impact location in TASER safety evaluations, contributing to a broader understanding of TASER cardiac risks and providing a basis to advocate for rigorous safety protocols.
{"title":"Evaluating cardiac risks of TASER: An in‐depth case study through probable current analysis","authors":"Hojeong Seo, Saehan Park, Sanghee Kim, Sookyoung Lee, Changho Choi","doi":"10.1111/1556-4029.15614","DOIUrl":"https://doi.org/10.1111/1556-4029.15614","url":null,"abstract":"This study investigates the cardiac safety concerns related to TASER discharges centering on a pivotal case that marked the first TASER‐related fatality in South Korea. Employing Pratt et al.'s theoretical framework, the research evaluates the potential for ventricular fibrillation (VF) from these discharges. The methodology incorporated a high‐resolution waveform analysis using sophisticated equipment and considered specific incident details, including dart impact locations verified through a forensic examination. A human body impedance of 500 Ω, chosen based on empirical studies and coupled with non‐inductive resistance for high‐voltage handling, was utilized in the model. By applying a heart‐current factor from IEC 60479 standards, the study found a VF risk of up to 5% depending on the impact location and current pathways. In this specific case, although the calculated risk did not exceed critical thresholds, the VF risk was high enough to suggest that TASER discharges played a role in the fatal outcome. This study underscores the importance of dart impact location in TASER safety evaluations, contributing to a broader understanding of TASER cardiac risks and providing a basis to advocate for rigorous safety protocols.","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"4 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142217127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anna Smędra MD, PhD, Katarzyna Wochna DMD, PhD, Jacek Gruchała MD, PhD, Jarosław Berent MD, PhD
Hypernatremia is an increase in serum sodium concentration above 145 mmol/L. There are many causes of elevated sodium levels in the blood serum. One is incorrect actions performed by medical staff. The symptoms of excessively high serum sodium levels depend on the severity of hypernatremia, the rate of its increase and the accompanying volume disorders. Severe symptoms include altered consciousness, increased muscle tone and reflexes, convulsions, psychomotor hyperactivity or drowsiness (up to coma), respiratory failure, and even death. We present the case of a 45-year-old man who took seven tablets of a blood pressure-lowering drug, and paramedics subsequently administered a concentrated solution of table salt to induce vomiting. However, vomiting did not occur, leading to hypernatremia. Ultimately, the man survived but developed persistent cognitive dysfunction, including disordered short-term memory and encoding and retrieval of information from long-term memory, weakening of attention function and fatigue, and disorders in abstract thinking. The patient's family went to the prosecutor's office to investigate the possibility of medical malpractice. Experts found that the paramedics' actions were incorrect. Although it has been known for many years that table salt solutions should not be used to induce vomiting, unfortunately, both laypeople and medical professionals are still using this technique. Iatrogenic salt poisoning may end not only in serious health complications but also in legal consequences.
{"title":"Poisoning with table salt while treating drug poisoning","authors":"Anna Smędra MD, PhD, Katarzyna Wochna DMD, PhD, Jacek Gruchała MD, PhD, Jarosław Berent MD, PhD","doi":"10.1111/1556-4029.15623","DOIUrl":"10.1111/1556-4029.15623","url":null,"abstract":"<p>Hypernatremia is an increase in serum sodium concentration above 145 mmol/L. There are many causes of elevated sodium levels in the blood serum. One is incorrect actions performed by medical staff. The symptoms of excessively high serum sodium levels depend on the severity of hypernatremia, the rate of its increase and the accompanying volume disorders. Severe symptoms include altered consciousness, increased muscle tone and reflexes, convulsions, psychomotor hyperactivity or drowsiness (up to coma), respiratory failure, and even death. We present the case of a 45-year-old man who took seven tablets of a blood pressure-lowering drug, and paramedics subsequently administered a concentrated solution of table salt to induce vomiting. However, vomiting did not occur, leading to hypernatremia. Ultimately, the man survived but developed persistent cognitive dysfunction, including disordered short-term memory and encoding and retrieval of information from long-term memory, weakening of attention function and fatigue, and disorders in abstract thinking. The patient's family went to the prosecutor's office to investigate the possibility of medical malpractice. Experts found that the paramedics' actions were incorrect. Although it has been known for many years that table salt solutions should not be used to induce vomiting, unfortunately, both laypeople and medical professionals are still using this technique. Iatrogenic salt poisoning may end not only in serious health complications but also in legal consequences.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2325-2330"},"PeriodicalIF":1.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142217141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Filicide is the act of a parent killing their own offspring. Previous studies indicate that there are both commonalities and distinctions between filicides committed by mothers and fathers. The main objective of this study was to compare maternal and paternal filicide with a major focus on clinical and sociodemographic features of perpetrators, incident details, and victims. Filicide cases were examined at the bedded unit of the Expertise Department of Observation of the Council of Forensic Medicine that were referred by the Turkish Ministry of Justice. A total of 51 perpetrators and 57 victims were analyzed from June 2014 to December 2023. The perpetrators were daily face-to-face interviewed by psychiatry and forensic medicine physicians. There were significant differences in sociodemographic characteristics and psychiatric conditions between maternal and paternal cases. Female perpetrators tended to be younger, unemployed, mostly targeted younger victims and used asphyxiation to kill, whereas males tended to target older victims and utilized firearms. The most common primary cause of filicide in maternal cases was “unwanted pregnancy,” whereas “spousal revenge” and “anger or impulsivity” were predominant in paternal filicides. All perpetrators who were not criminally responsible were mothers, mostly diagnosed with psychotic depression. Unwanted pregnancy, psychotic depression, and postpartum psychosis should be assessed when evaluating risk for mothers, especially for infanticide. The desire for revenge on a spouse, often seen with personality disorders, impulsivity and anger, access to firearms should be screened for risk of paternal filicide, particularly in older victims. Early recognition of these factors can help in implementing prevention strategies.
{"title":"Patterns in paternal and maternal filicide: A comparative analysis of filicide cases in Turkey","authors":"Tuba Özcanlı MD, İhsan Okur MD, Cana Aksoy Poyraz MD, Neşe Kocabaşoğlu MD, Hızır Aslıyüksek MD","doi":"10.1111/1556-4029.15625","DOIUrl":"10.1111/1556-4029.15625","url":null,"abstract":"<p>Filicide is the act of a parent killing their own offspring. Previous studies indicate that there are both commonalities and distinctions between filicides committed by mothers and fathers. The main objective of this study was to compare maternal and paternal filicide with a major focus on clinical and sociodemographic features of perpetrators, incident details, and victims. Filicide cases were examined at the bedded unit of the Expertise Department of Observation of the Council of Forensic Medicine that were referred by the Turkish Ministry of Justice. A total of 51 perpetrators and 57 victims were analyzed from June 2014 to December 2023. The perpetrators were daily face-to-face interviewed by psychiatry and forensic medicine physicians. There were significant differences in sociodemographic characteristics and psychiatric conditions between maternal and paternal cases. Female perpetrators tended to be younger, unemployed, mostly targeted younger victims and used asphyxiation to kill, whereas males tended to target older victims and utilized firearms. The most common primary cause of filicide in maternal cases was “unwanted pregnancy,” whereas “spousal revenge” and “anger or impulsivity” were predominant in paternal filicides. All perpetrators who were not criminally responsible were mothers, mostly diagnosed with psychotic depression. Unwanted pregnancy, psychotic depression, and postpartum psychosis should be assessed when evaluating risk for mothers, especially for infanticide. The desire for revenge on a spouse, often seen with personality disorders, impulsivity and anger, access to firearms should be screened for risk of paternal filicide, particularly in older victims. Early recognition of these factors can help in implementing prevention strategies.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2110-2119"},"PeriodicalIF":1.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15625","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142217128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this article, we have explored the use of Mueller polarimetry for the detection and enhancement of alterations in questioned documents. Erasures, obliterations (with liquid paper and by pasting an additional layer of paper), and insertions (made with several inks) were studied in both regular and glossy paper. Promising results were obtained, which depend on the type of paper and the relation between the color of the ink and that of the illuminating light source. Erasures are easier to detect in glossy paper than in regular paper. Obliterations with liquid paper produced successful results in both types of paper, while detection of obliterations made with an additional layer of paper led to higher contrast for regular paper. Regarding the insertions, the black ball-point ink could be differentiated from roller-ball and gel-pen ink, which is often difficult to achieve visually. The contrast observed between the two inks was higher for regular paper than for glossy paper. Although the results shown in this article are promising, a wider variety of papers and pen types must be tested to further develop the procedure. It has the advantage of being non-destructive and far more economic than other methods. In some cases, the results can be complementary to those obtained by other methods (e.g., fluorescence with UV excitation and illumination with transmitted and oblique light), while in other cases the method offers unique advantages.
{"title":"Visual detection and enhancement of alterations in questioned documents by Mueller polarimetry","authors":"Emanuel Chironi PhD, Claudio Iemmi PhD","doi":"10.1111/1556-4029.15617","DOIUrl":"10.1111/1556-4029.15617","url":null,"abstract":"<p>In this article, we have explored the use of Mueller polarimetry for the detection and enhancement of alterations in questioned documents. Erasures, obliterations (with liquid paper and by pasting an additional layer of paper), and insertions (made with several inks) were studied in both regular and glossy paper. Promising results were obtained, which depend on the type of paper and the relation between the color of the ink and that of the illuminating light source. Erasures are easier to detect in glossy paper than in regular paper. Obliterations with liquid paper produced successful results in both types of paper, while detection of obliterations made with an additional layer of paper led to higher contrast for regular paper. Regarding the insertions, the black ball-point ink could be differentiated from roller-ball and gel-pen ink, which is often difficult to achieve visually. The contrast observed between the two inks was higher for regular paper than for glossy paper. Although the results shown in this article are promising, a wider variety of papers and pen types must be tested to further develop the procedure. It has the advantage of being non-destructive and far more economic than other methods. In some cases, the results can be complementary to those obtained by other methods (e.g., fluorescence with UV excitation and illumination with transmitted and oblique light), while in other cases the method offers unique advantages.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2275-2289"},"PeriodicalIF":1.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morgan Eaton BS, M. Shane Woolf PhD, Samyuktha Pemmasani, Triniti Turner BS, Janina Golob Deeb DMD, MS, Tracey Dawson Green PhD
Disaster victim identification (DVI) refers to the forensic identification of unknown individuals following a mass disaster event. Human dental structures can contain viable DNA sources when other soft tissues are compromised. However, labor-intensive sample preparation performed by extensively trained personnel is needed to expose the nuclear material for traditional forensic DNA workflows. With this in mind, we evaluated two simplified sample preparation protocols for processing tooth samples using either a conventional forensic DNA workflow or the Applied Biosystems® RapidHIT™ ID instrument. Briefly, sample sets for both protocols included 10 deciduous teeth that were cleaned prior to either fragmentation with a claw hammer (for RapidHIT™ ID processing) or fine-powder pulverization with a consumer-grade coffee grinder (for traditional workflows). The average percentage of expected STR alleles that were detected above analytical threshold for these tooth samples were comparable between methods: RapidHIT™ ID = 99.0% and GlobalFiler™ = 99.8%. Average intralocus heterozygote peak height ratios (PHRs) were comparable: RapidHIT™ ID = 0.80 and GlobalFiler™ = 0.86. Importantly, 9 of 10 samples analyzed via the RapidHIT™ ID required analyst review for flagged artifact peaks and quality issues. Across all profiles, 91% of alleles passed quality metrics for the RapidHIT™ workflow versus 100% for conventional GlobalFiler™ analysis. Collectively, these results suggest that quick, low-tech tooth sample fragmentation followed by analysis with the RapidHIT™ ID instrument can produce complete STR profiles from aged tooth samples. Future studies should include larger samples sets, more challenging tooth samples, and further simplification of sample preparation to enable field-forward, on-scene DVI.
灾难受害者身份鉴定(DVI)是指对大规模灾难事件后的未知人员进行法医鉴定。当其他软组织受到破坏时,人类牙齿结构中可能含有可行的 DNA 来源。然而,传统的法医 DNA 工作流程需要经过广泛培训的人员进行劳动密集型样本制备,以暴露核材料。有鉴于此,我们评估了使用传统法医 DNA 工作流程或 Applied Biosystems® RapidHIT™ ID 仪器处理牙齿样本的两种简化样本制备方案。简而言之,两种方案的样本集都包括 10 颗乳牙,在用爪锤破碎(用于 RapidHIT™ ID 处理)或用消费级咖啡研磨机细粉粉碎(用于传统工作流程)之前都要进行清洁。在这些牙齿样本中,检测到的高于分析阈值的预期 STR 等位基因的平均百分比在不同方法之间具有可比性:RapidHIT™ ID = 99.0% 和 GlobalFiler™ = 99.8%。病灶内杂合峰高比 (PHR) 平均值相当:RapidHIT™ ID = 0.80 和 GlobalFiler™ = 0.86。重要的是,在通过 RapidHIT™ ID 分析的 10 个样本中,有 9 个样本需要分析师对标记的伪峰和质量问题进行审查。在所有图谱中,RapidHIT™ 工作流程有 91% 的等位基因通过了质量指标检测,而传统 GlobalFiler™ 分析的等位基因通过率为 100%。总之,这些结果表明,快速、低技术含量的牙齿样本破碎,然后使用 RapidHIT™ ID 仪器进行分析,可以从陈旧的牙齿样本中得到完整的 STR 图谱。未来的研究应包括更大的样本集、更具挑战性的牙齿样本,并进一步简化样本制备,以实现现场直接 DVI。
{"title":"Two simplified tooth sample preparation methods for conventional laboratory and RapidHIT™ ID workflows","authors":"Morgan Eaton BS, M. Shane Woolf PhD, Samyuktha Pemmasani, Triniti Turner BS, Janina Golob Deeb DMD, MS, Tracey Dawson Green PhD","doi":"10.1111/1556-4029.15624","DOIUrl":"10.1111/1556-4029.15624","url":null,"abstract":"<p>Disaster victim identification (DVI) refers to the forensic identification of unknown individuals following a mass disaster event. Human dental structures can contain viable DNA sources when other soft tissues are compromised. However, labor-intensive sample preparation performed by extensively trained personnel is needed to expose the nuclear material for traditional forensic DNA workflows. With this in mind, we evaluated two simplified sample preparation protocols for processing tooth samples using either a conventional forensic DNA workflow or the Applied Biosystems® RapidHIT™ ID instrument. Briefly, sample sets for both protocols included 10 deciduous teeth that were cleaned prior to either fragmentation with a claw hammer (for RapidHIT™ ID processing) or fine-powder pulverization with a consumer-grade coffee grinder (for traditional workflows). The average percentage of expected STR alleles that were detected above analytical threshold for these tooth samples were comparable between methods: RapidHIT™ ID = 99.0% and GlobalFiler™ = 99.8%. Average intralocus heterozygote peak height ratios (PHRs) were comparable: RapidHIT™ ID = 0.80 and GlobalFiler™ = 0.86. Importantly, 9 of 10 samples analyzed via the RapidHIT™ ID required analyst review for flagged artifact peaks and quality issues. Across all profiles, 91% of alleles passed quality metrics for the RapidHIT™ workflow versus 100% for conventional GlobalFiler™ analysis. Collectively, these results suggest that quick, low-tech tooth sample fragmentation followed by analysis with the RapidHIT™ ID instrument can produce complete STR profiles from aged tooth samples. Future studies should include larger samples sets, more challenging tooth samples, and further simplification of sample preparation to enable field-forward, on-scene DVI.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2197-2208"},"PeriodicalIF":1.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15624","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aidan Armstrong, Kennedy O. Doro PhD, Katrina Cristino, Agathe Ribéreau-Gayon PhD, Shari L. Forbes PhD, William T. D. Wadsworth MA, Carl-Georg Bank PhD
Studies assessing the use of ground-penetrating radar (GPR) for locating unmarked human graves commonly use pigs as proxies, with recent concerns about the adequacy of pigs as substitutes for humans. Also, there is little agreement on how to identify and describe GPR signals associated with graves. Hence, this project's aim is to compare GPR signals acquired over simulated clandestine graves with pig and human remains. We established human, pig, and control graves at the REST[ES] human decomposition facility in May 2022 and monitored the graves over 17 months using a 250 MHz antenna GPR system. Our results showed the presence of perturbed and V-shaped reflectors, diffraction hyperbolas, and reflectors with amplitude loss at depth between 0.6 and 0.75 m in the radargram for graves with human and pig remains. We corroborate recent studies which concluded that the use of proxies is a viable alternative to human cadavers. The observed radar signatures were classified into five key patterns, which are characteristic of similar data collected with 250 MHz above graves reported in the literature. These classes are: V-shaped dipping reflections from grave walls (class A), small hyperbolic reflections superimposed onto a near-linear reflector (class B), hyperbolic reflections from remains within the grave (class C), new high-amplitude reflection patterns (class D) and significant loss or interruption of reflections (class E). Our proposed classification can help streamline future investigations where the goal is to interpret burials within large GPR datasets and provide language to communicate these results to the broader scientific community.
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Lee LYC, Lee YS, Tan J, Lee JY, Syn CK. A study of DNA transfers onto plastic packets placed in personal bags. J Forensic Sci. 2024;69(2):430–6. https://doi.org/10.1111/1556-4029.15460
In section 2.2 DNA Processing, there is a missing character ‘1’ immediately after ‘3 to’ in the text.
The current text reads: “In general, the proportion of major to minor contributors should be at least 3 to and any profile with three or more contributors at an indistinct level would be deemed not interpretable.”
The correct text should read: “In general, the proportion of major to minor contributors should be at least 3 to 1 and any profile with three or more contributors at an indistinct level would be deemed not interpretable.”
We apologize for this error.
Lee LYC, Lee YS, Tan J, Lee JY, Syn CK.DNA转移到个人包中塑料袋上的研究。J Forensic Sci. 2024;69(2):430-6。https://doi.org/10.1111/1556-4029.15460In 第 2.2 节 DNA 处理中,"3 to "后面少了一个字符 "1"。现在的内容是:"一般来说,主要贡献者与次要贡献者的比例至少应为 3 比,任何有 3 个或更多贡献者的图谱水平不明确,将被视为不可解释。"正确的文字应为:"一般来说,主要贡献者与次要贡献者的比例至少应为 3 比:"一般来说,主要撰稿人与次要撰稿人的比例应至少为 3 比 1,任何有三个或更多撰稿人的简介,其层次不明确,将被视为不可解释。"我们对这一错误表示歉意。
{"title":"Correction to: A study of DNA transfers onto plastic packets placed in personal bags","authors":"","doi":"10.1111/1556-4029.15618","DOIUrl":"10.1111/1556-4029.15618","url":null,"abstract":"<p>Lee LYC, Lee YS, Tan J, Lee JY, Syn CK. A study of DNA transfers onto plastic packets placed in personal bags. J Forensic Sci. 2024;69(2):430–6. https://doi.org/10.1111/1556-4029.15460</p><p>In section 2.2 DNA Processing, there is a missing character ‘1’ immediately after ‘3 to’ in the text.</p><p>The current text reads: “In general, the proportion of major to minor contributors should be at least 3 to and any profile with three or more contributors at an indistinct level would be deemed not interpretable.”</p><p>The correct text should read: “In general, the proportion of major to minor contributors should be at least 3 to 1 and any profile with three or more contributors at an indistinct level would be deemed not interpretable.”</p><p>We apologize for this error.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2351"},"PeriodicalIF":1.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1556-4029.15618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruoyu Huang MD, Qiuyu Pang MD, Lexin Zheng MD, Rui Duan BSc, Ying Wang PhD, Zhiyong Wang BSc, Tao Wang MD, PhD
Diester diterpenoid alkaloids (DDAs) are the main active ingredients of herbaceous perennial plants Aconitum. DDAs possess cardiotoxic and neurotoxic properties. Although most deaths caused by DDA poisoning are accidental, a few instances of suicide and homicide have been reported. Presented is a case of an acute aconitine (AC) poisoning following the ingestion of approximately 50 mL of homemade medicinal liquor. We described the clinical manifestations after poisoning and detailed postmortem changes, and detected the concentrations of AC and hypaconitine (HA) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The decedent experienced a burning sensation in the gastrointestinal tract after poisoning, followed by flushing and paralysis of the face and limbs, and severe cardiac arrhythmia. An autopsy revealed cyanosis of the lips and nail beds; conjunctival hemorrhage in both eyes; pulmonary edema; tissue hemorrhage and congestion in multiple organs; and inflammatory cell infiltration in the stomach, duodenum, pancreas, and cardiac muscle. The concentrations of AC and HA were as follows: cardiac blood, 38.4 ng/mL and 7.1 ng/mL; pericardial fluid, 7.3 ng/mL and 41 ng/mL; urine, 28.1 ng/mL and 574 ng/mL; bile, 38.5 ng/mL and 108 ng/mL; gastric contents, 0.06 mg and 0.56 mg; liver tissue, 10.7 ng/g and 109.6 ng/g; and medicinal liquor, 0.568 mg/mL and 0.664 mg/mL, respectively. The clinical manifestations, anatomy findings, and quantitative data on the concentrations of AC and HA in body fluids and tissues will aid forensic investigations of deaths caused by acute AC poisoning.
二酯二萜生物碱(DDAs)是多年生草本植物乌头的主要活性成分。DDAs 具有心脏毒性和神经毒性。虽然大多数 DDA 中毒导致的死亡都是意外事故,但也有少数自杀和他杀的报道。本文介绍了一例因摄入约 50 毫升自制药酒而导致急性乌头碱(AC)中毒的病例。我们描述了中毒后的临床表现和详细的尸检变化,并通过液相色谱-串联质谱法(LC-MS/MS)检测了乌头碱和次乌头碱(HA)的浓度。死者中毒后胃肠道有灼烧感,随后面部和四肢潮红、麻痹,并出现严重的心律失常。尸检结果显示:嘴唇和甲床发绀;双眼结膜出血;肺水肿;多个器官组织出血和充血;胃、十二指肠、胰腺和心肌有炎症细胞浸润。AC 和 HA 的浓度如下:心血,38.4 纳克/毫升和 7.1 纳克/毫升;心包液,7.3 纳克/毫升和 41 纳克/毫升;尿液,28.1 纳克/毫升和 574 纳克/毫升;胆汁,38.5纳克/毫升和108纳克/毫升;胃内容物,分别为0.06毫克和0.56毫克;肝组织,分别为10.7纳克/克和109.6纳克/克;药液,分别为0.568毫克/毫升和0.664毫克/毫升。临床表现、解剖结果以及体液和组织中 AC 和 HA 浓度的定量数据将有助于对急性 AC 中毒导致的死亡进行法医调查。
{"title":"Acute aconitine poisoning resulting from the ingestion of medicinal liquor","authors":"Ruoyu Huang MD, Qiuyu Pang MD, Lexin Zheng MD, Rui Duan BSc, Ying Wang PhD, Zhiyong Wang BSc, Tao Wang MD, PhD","doi":"10.1111/1556-4029.15621","DOIUrl":"10.1111/1556-4029.15621","url":null,"abstract":"<p>Diester diterpenoid alkaloids (DDAs) are the main active ingredients of herbaceous perennial plants <i>Aconitum</i>. DDAs possess cardiotoxic and neurotoxic properties. Although most deaths caused by DDA poisoning are accidental, a few instances of suicide and homicide have been reported. Presented is a case of an acute aconitine (AC) poisoning following the ingestion of approximately 50 mL of homemade medicinal liquor. We described the clinical manifestations after poisoning and detailed postmortem changes, and detected the concentrations of AC and hypaconitine (HA) by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The decedent experienced a burning sensation in the gastrointestinal tract after poisoning, followed by flushing and paralysis of the face and limbs, and severe cardiac arrhythmia. An autopsy revealed cyanosis of the lips and nail beds; conjunctival hemorrhage in both eyes; pulmonary edema; tissue hemorrhage and congestion in multiple organs; and inflammatory cell infiltration in the stomach, duodenum, pancreas, and cardiac muscle. The concentrations of AC and HA were as follows: cardiac blood, 38.4 ng/mL and 7.1 ng/mL; pericardial fluid, 7.3 ng/mL and 41 ng/mL; urine, 28.1 ng/mL and 574 ng/mL; bile, 38.5 ng/mL and 108 ng/mL; gastric contents, 0.06 mg and 0.56 mg; liver tissue, 10.7 ng/g and 109.6 ng/g; and medicinal liquor, 0.568 mg/mL and 0.664 mg/mL, respectively. The clinical manifestations, anatomy findings, and quantitative data on the concentrations of AC and HA in body fluids and tissues will aid forensic investigations of deaths caused by acute AC poisoning.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"69 6","pages":"2317-2324"},"PeriodicalIF":1.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}