Pub Date : 2024-10-04DOI: 10.1007/s10895-024-03955-8
Srinath, Omnath Patil, Sulochana Devar, S M Hanagodimath
At room temperature, the absorption and fluorescence properties of coumarin 6-Methoxy-4-(4-nitro-phenoxy methyl)-chromen-2-one (6MNPM) are investigated in pure organic solvents and a combination of acetonitrile (ACN) and tetrahydrofuran (THF). The pure solvents' influence on spectral characteristics is examined by applying theories such as Kamlet and Catalan's multiple linear regression techniques, Reichardt's microscopic solvent polarity parameter, and the Lippert-Mataga polarity function. The significant role of solute-solvent interactions in pure solvents, particularly dielectric interaction and hydrogen bonding. However, hydrogen bonding interactions dominate the contribution of dielectric interactions. The electric dipole moments of both the ground as well as excited states had been calculated using the Solvatochromic method. The value of the excited state electric dipole moment and the redshifts of the emission spectra show that the emitting singlet state has an intramolecular charge transfer (ICT) character. From Catalan's linear regression, we found that di-polarity has a much smaller influence than polarizability. By solvation study, we conclude that Tetrahydrofuran solvent is preferred over Acetonitrile.
{"title":"Estimation of Electric Dipole Moment by Solvatochromism, Computational Method, and Study of the Effect of Solvents by Preferential Solvation of 6 - Methoxy-4-(4-Nitro-Phenoxy Methyl)-Chromen-2-One (6mnpm).","authors":"Srinath, Omnath Patil, Sulochana Devar, S M Hanagodimath","doi":"10.1007/s10895-024-03955-8","DOIUrl":"https://doi.org/10.1007/s10895-024-03955-8","url":null,"abstract":"<p><p>At room temperature, the absorption and fluorescence properties of coumarin 6-Methoxy-4-(4-nitro-phenoxy methyl)-chromen-2-one (6MNPM) are investigated in pure organic solvents and a combination of acetonitrile (ACN) and tetrahydrofuran (THF). The pure solvents' influence on spectral characteristics is examined by applying theories such as Kamlet and Catalan's multiple linear regression techniques, Reichardt's microscopic solvent polarity parameter, and the Lippert-Mataga polarity function. The significant role of solute-solvent interactions in pure solvents, particularly dielectric interaction and hydrogen bonding. However, hydrogen bonding interactions dominate the contribution of dielectric interactions. The electric dipole moments of both the ground as well as excited states had been calculated using the Solvatochromic method. The value of the excited state electric dipole moment and the redshifts of the emission spectra show that the emitting singlet state has an intramolecular charge transfer (ICT) character. From Catalan's linear regression, we found that di-polarity has a much smaller influence than polarizability. By solvation study, we conclude that Tetrahydrofuran solvent is preferred over Acetonitrile.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-03DOI: 10.1007/s10895-024-03982-5
Afrasiab Salehi Moghanlou, Mehdi Molaei, Tao Fang
In the present study, Rgo/Fe3O4/CdSe as a dark catalyst material was synthesized by a refluxing method. The synthesized magnetic nanocomposites were studied by various analyses such as Fourier transform infrared (FTIR), energy-dispersive X-ray spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffractometer (XRD), Raman, Zeta and vibrating sample magnetometer (VSM). Characterization of structural analysis showed that the nanocomposites were successfully synthesized. The absorption spectrum was used to determine the dark catalyst activity of rGO/Fe3O4/CdSe nanocomposite. Analysis of the absorption spectrum showed that the prepared nanocomposites degrade the MB organic dye completely (100%) after 2 min of stirring in the dark, also experimenting with different pH showed that the best performance for the degradation of MB occurs in neutral and alkaline media. The Raman spectrum analysis showed that the Fe3O4/CdSe quantum dots (QDs) were correctly incorporated on the reduced graphene oxide (rGO) nanosheets. Zeta potential analysis showed that rGO/Fe3O4/CdSe has a large amount of negative charge on its surface and the surface charge increased by about 16 mV compared to the Fe3O4/CdSe compound. BET and BJH techniques were used to determine the effective surface area and pore size diameter, BET results to determine the effective surface area showed that by adding graphene to the compound, the specific surface area increased from 42.877 m2g-1 to 54.1896 m2g-1. The radical scavenger experiment showed that electrons play an essential role in the degradation process. VSM analysis showed that the prepared nanocomposites have excellent superparamagnetic behavior, this advantage enables the easy collection of nanocatalysts by magnets from wastewater after dye degradation.
{"title":"A New Powerful Magnetic Dark Catalyst Based on rGO/Fe<sub>3</sub>O<sub>4</sub>/CdSe Nanocomposite for Ultrafast Degradation of Methylene Blue Dye.","authors":"Afrasiab Salehi Moghanlou, Mehdi Molaei, Tao Fang","doi":"10.1007/s10895-024-03982-5","DOIUrl":"https://doi.org/10.1007/s10895-024-03982-5","url":null,"abstract":"<p><p>In the present study, Rgo/Fe<sub>3</sub>O<sub>4</sub>/CdSe as a dark catalyst material was synthesized by a refluxing method. The synthesized magnetic nanocomposites were studied by various analyses such as Fourier transform infrared (FTIR), energy-dispersive X-ray spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffractometer (XRD), Raman, Zeta and vibrating sample magnetometer (VSM). Characterization of structural analysis showed that the nanocomposites were successfully synthesized. The absorption spectrum was used to determine the dark catalyst activity of rGO/Fe<sub>3</sub>O<sub>4</sub>/CdSe nanocomposite. Analysis of the absorption spectrum showed that the prepared nanocomposites degrade the MB organic dye completely (100%) after 2 min of stirring in the dark, also experimenting with different pH showed that the best performance for the degradation of MB occurs in neutral and alkaline media. The Raman spectrum analysis showed that the Fe<sub>3</sub>O<sub>4</sub>/CdSe quantum dots (QDs) were correctly incorporated on the reduced graphene oxide (rGO) nanosheets. Zeta potential analysis showed that rGO/Fe<sub>3</sub>O<sub>4</sub>/CdSe has a large amount of negative charge on its surface and the surface charge increased by about 16 mV compared to the Fe<sub>3</sub>O<sub>4</sub>/CdSe compound. BET and BJH techniques were used to determine the effective surface area and pore size diameter, BET results to determine the effective surface area showed that by adding graphene to the compound, the specific surface area increased from 42.877 m<sup>2</sup>g<sup>-1</sup> to 54.1896 m<sup>2</sup>g<sup>-1</sup>. The radical scavenger experiment showed that electrons play an essential role in the degradation process. VSM analysis showed that the prepared nanocomposites have excellent superparamagnetic behavior, this advantage enables the easy collection of nanocatalysts by magnets from wastewater after dye degradation.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1007/s10895-024-03971-8
Marco A Saraiva
The primary objective of this research is to further examine the events occurring during the active or burst phase by focusing on the aggregation of the Syn amyloid protein. Regarding this aspect, it was initially conducted rapid temperature variations using stopped-flow spectrometry and tyrosyl group fluorescence emission detection, within the initial 500 milliseconds in buffered Syn solutions at pH 7, exploring various temperature ranges to investigate protein aggregation. The results obtained were contrasted with results obtained for the Nα-acetyl-L-tyrosinamide (NAYA) parent compound in the same conditions. The utilization of the NAYA compound is suitable as it mimics the peptide bonds in proteins and contains a tyrosyl group resembling the four tyrosyl groups found in the Syn protein structure (the protein has no tryptophan residues). Furthermore, the NAYA compound adopts an intramolecularly hydrogen-bonded structure even in an aqueous solution, similar to the interactions seen in the hydrophilic face of β-sheets. Additionally, the Syn protein system can exhibit the presence of β-sheets as a result of the existence of very low abundant Syn amyloid precursor forms or nuclei during the initial stages of the protein aggregation. Thus, a relationship is present between the molecular processes in the NAYA and Syn protein systems, making the NAYA's application crucial in this research. Moreover, to aid in understanding the results, it was also compared the events during the quiescent or inactive phase (30-500 milliseconds) with those in the burst phase (up to 10 milliseconds) using stopped-flow spectrometry conditions. Steady-state measurements were beneficial in comprehending the occurrences in both the quiescent and burst phases examined. Although protein aggregation and disaggregation were observed during the quiescent phase, determining these processes in the burst phase was more challenging. In the latter case, the aggregation of the Syn protein is actually initiated by the interaction of the intrinsically disordered Syn monomers. In the quiescent phase, first-order rate constants were measured and analysis showed that Syn protein aggregation and disaggregation occur simultaneously. At lower temperatures, early protein disaggregation outweighs protein aggregation whereas at higher temperatures protein disaggregation and aggregation are rather similar. It is also need to highlight that the burst phase, while distinct from the quiescent phase, can be considered as a possible structural phase for obtaining details about the aggregation of this specific disordered protein in solution on a very short timescale.
这项研究的主要目的是通过关注 Syn 淀粉样蛋白的聚集,进一步研究在活性或爆发阶段发生的事件。在这方面,研究人员首先使用停流光谱仪和酪氨酰基荧光发射检测技术,在 pH 值为 7 的缓冲 Syn 溶液中的最初 500 毫秒内进行快速温度变化,探索各种温度范围以研究蛋白质的聚集情况。获得的结果与 Nα- 乙酰基-L-酪氨酰胺(NAYA)母体化合物在相同条件下获得的结果进行了对比。使用 NAYA 化合物是合适的,因为它模仿了蛋白质中的肽键,并含有一个与 Syn 蛋白结构中的四个酪氨酰基相似的酪氨酰基(蛋白质中没有色氨酸残基)。此外,即使在水溶液中,NAYA 化合物也能形成分子内氢键结构,类似于 β 片层亲水面的相互作用。此外,在蛋白质聚集的初始阶段,由于存在含量极低的 Syn 淀粉样蛋白前体或核,Syn 蛋白系统也会显示出 β 片层的存在。因此,NAYA 和 Syn 蛋白系统中的分子过程之间存在着某种关系,这使得 NAYA 在本研究中的应用变得至关重要。此外,为了帮助理解研究结果,研究人员还利用停流光谱条件比较了静态或非活动期(30-500 毫秒)与爆发期(最多 10 毫秒)的事件。稳态测量有利于理解静态和爆发阶段的事件。虽然在静止期观察到了蛋白质聚集和分解,但在爆发期确定这些过程更具挑战性。在后一种情况下,Syn 蛋白的聚集实际上是由本质上无序的 Syn 单体相互作用引发的。在静止阶段,测量了一阶速率常数,分析表明 Syn 蛋白的聚集和分解是同时发生的。在较低温度下,早期蛋白质的解聚大于蛋白质的聚集,而在较高温度下,蛋白质的解聚和聚集相当接近。还需要强调的是,尽管猝灭阶段有别于静止阶段,但可以将其视为一种可能的结构阶段,以便在极短的时间尺度内获得这种特定无序蛋白质在溶液中聚集的细节。
{"title":"Temperature-Driven Stopped-Flow Experiments for Investigating the Initial Aggregation of the α-Synuclein Amyloid Protein, Focusing on Active and Inactive Phases.","authors":"Marco A Saraiva","doi":"10.1007/s10895-024-03971-8","DOIUrl":"https://doi.org/10.1007/s10895-024-03971-8","url":null,"abstract":"<p><p>The primary objective of this research is to further examine the events occurring during the active or burst phase by focusing on the aggregation of the Syn amyloid protein. Regarding this aspect, it was initially conducted rapid temperature variations using stopped-flow spectrometry and tyrosyl group fluorescence emission detection, within the initial 500 milliseconds in buffered Syn solutions at pH 7, exploring various temperature ranges to investigate protein aggregation. The results obtained were contrasted with results obtained for the N<sub>α</sub>-acetyl-L-tyrosinamide (NAYA) parent compound in the same conditions. The utilization of the NAYA compound is suitable as it mimics the peptide bonds in proteins and contains a tyrosyl group resembling the four tyrosyl groups found in the Syn protein structure (the protein has no tryptophan residues). Furthermore, the NAYA compound adopts an intramolecularly hydrogen-bonded structure even in an aqueous solution, similar to the interactions seen in the hydrophilic face of β-sheets. Additionally, the Syn protein system can exhibit the presence of β-sheets as a result of the existence of very low abundant Syn amyloid precursor forms or nuclei during the initial stages of the protein aggregation. Thus, a relationship is present between the molecular processes in the NAYA and Syn protein systems, making the NAYA's application crucial in this research. Moreover, to aid in understanding the results, it was also compared the events during the quiescent or inactive phase (30-500 milliseconds) with those in the burst phase (up to 10 milliseconds) using stopped-flow spectrometry conditions. Steady-state measurements were beneficial in comprehending the occurrences in both the quiescent and burst phases examined. Although protein aggregation and disaggregation were observed during the quiescent phase, determining these processes in the burst phase was more challenging. In the latter case, the aggregation of the Syn protein is actually initiated by the interaction of the intrinsically disordered Syn monomers. In the quiescent phase, first-order rate constants were measured and analysis showed that Syn protein aggregation and disaggregation occur simultaneously. At lower temperatures, early protein disaggregation outweighs protein aggregation whereas at higher temperatures protein disaggregation and aggregation are rather similar. It is also need to highlight that the burst phase, while distinct from the quiescent phase, can be considered as a possible structural phase for obtaining details about the aggregation of this specific disordered protein in solution on a very short timescale.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1007/s10895-024-03951-y
Anil Kumar, C G Renuka
Temperature-dependent rotational dynamics of 3-(1 H-benzo[d]imidazol-2-yl)-7-(diethyl amino)-2 H-chromen-2-one (3HBI-7D-2HChromen-2-one) has been studied in highly viscous glycerol solvent. Over the entire studied temperature range rotational reorientation time values vary linearly with temperature. Experimental obtained rotational results are analyzed using hydrodynamic and quasi-hydrodynamic models over the entire temperature range as well as low and high viscous range. 3HBI-7D-2HChromen-2-one rotational dynamics has been described using Stoke's-Einstein-Debye (SED) slip boundary conditions.
{"title":"Influence of Viscosity and Temperature on Rotational Dynamics of 3-(1H-benzo[d]Imidazol-2-yl)-7-(Diethyl Amino)-2H-chromen-2-one (Polar Laser dye) in Highly Viscous Solvent: Low and High Viscous Range.","authors":"Anil Kumar, C G Renuka","doi":"10.1007/s10895-024-03951-y","DOIUrl":"10.1007/s10895-024-03951-y","url":null,"abstract":"<p><p>Temperature-dependent rotational dynamics of 3-(1 H-benzo[d]imidazol-2-yl)-7-(diethyl amino)-2 H-chromen-2-one (3HBI-7D-2HChromen-2-one) has been studied in highly viscous glycerol solvent. Over the entire studied temperature range rotational reorientation time values vary linearly with temperature. Experimental obtained rotational results are analyzed using hydrodynamic and quasi-hydrodynamic models over the entire temperature range as well as low and high viscous range. 3HBI-7D-2HChromen-2-one rotational dynamics has been described using Stoke's-Einstein-Debye (SED) slip boundary conditions.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1007/s10895-024-03977-2
Li Liu, Tao Zhou, Yawen Li, Tianrong Li
The number of "turn-on" fluorescent probes for Cu2+ is relatively limited, and interference from other metal cations presents a significant challenge for these sensors. In this study, we synthesized and characterized a rhodamine B-based sensor, designated as RBHP, using 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (PMBP) and rhodamine B hydrazide. Selectivity, sensitivity, solvent effects, water content, and pH of RBHP in relation to Cu²⁺ were conducted. RBHP exhibited an exceptionally low fluorescence background signal in acetonitrile and demonstrated a "turn-on" fluorescent response to Cu²⁺. The PMBP-based acylhydrazone moiety and acetonitrile as the detection solvent are crucial for the selective detection. RBHP shows potential as a highly selective and sensitive fluorescent sensor for Cu2+.
{"title":"A Novel Rhodamine B Derivative as a \"Turn-on\" Fluorescent Sensor for Cu<sup>2+</sup> with High Selectivity and Sensitivity.","authors":"Li Liu, Tao Zhou, Yawen Li, Tianrong Li","doi":"10.1007/s10895-024-03977-2","DOIUrl":"https://doi.org/10.1007/s10895-024-03977-2","url":null,"abstract":"<p><p>The number of \"turn-on\" fluorescent probes for Cu<sup>2+</sup> is relatively limited, and interference from other metal cations presents a significant challenge for these sensors. In this study, we synthesized and characterized a rhodamine B-based sensor, designated as RBHP, using 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (PMBP) and rhodamine B hydrazide. Selectivity, sensitivity, solvent effects, water content, and pH of RBHP in relation to Cu²⁺ were conducted. RBHP exhibited an exceptionally low fluorescence background signal in acetonitrile and demonstrated a \"turn-on\" fluorescent response to Cu²⁺. The PMBP-based acylhydrazone moiety and acetonitrile as the detection solvent are crucial for the selective detection. RBHP shows potential as a highly selective and sensitive fluorescent sensor for Cu<sup>2+</sup>.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1007/s10895-024-03980-7
Artem S Sherudillo, Alexander A Kalyagin, Lubov A Antina, Mikhail B Berezin, Elena V Antina
Aggregation-caused quenching effect (ACQ) greatly limits the practical use of many organic luminophores in biomedicine, optics and electronics. The comparative analysis of aggregation characteristics of CHR-bis(BODIPY) bichromophores 1-6 with R = H, Ph, MeOPh and various linking positions (α,α-; α,β-; β,β- and β',β'-) in THF-water mixtures with different water fractions or dye concentrations is first presented in this article. Both the linking style 1-4 and the arylation of the spacer with phenyl (Ph-) 5 or methoxyphenyl (MeOPh-) 6 substituents strongly affect the formation of luminophore aggregated forms in binary THF-water mixtures. The α,α-and β,β-isomers (1 and 3) form non-fluorescent H-type aggregates in THF-water mixtures with fw > 70%. The α,β-; β',β'-isomers (2, 4) and the MeOPh-substituted β,β-bichromophore 6 are characterized by predominant formation fluorescent aggregates. All bichromophores are characterized by the presence of residual amounts of non-aggregated forms in binary mixtures with maximum water content. The results are useful for controlling the aggregation behavior and spectral characteristics of CHR-bis(BODIPY) bichromophores in aqueous-organic media, which is important in the development of biomarkers and PDT agents.
{"title":"Aggregation Behavior of CHR-bis(BODIPY) Bichromophores in THF-water Mixtures: Effect of Linking Positions and Aryl-spacer Substituents.","authors":"Artem S Sherudillo, Alexander A Kalyagin, Lubov A Antina, Mikhail B Berezin, Elena V Antina","doi":"10.1007/s10895-024-03980-7","DOIUrl":"https://doi.org/10.1007/s10895-024-03980-7","url":null,"abstract":"<p><p>Aggregation-caused quenching effect (ACQ) greatly limits the practical use of many organic luminophores in biomedicine, optics and electronics. The comparative analysis of aggregation characteristics of CHR-bis(BODIPY) bichromophores 1-6 with R = H, Ph, MeOPh and various linking positions (α,α-; α,β-; β,β- and β',β'-) in THF-water mixtures with different water fractions or dye concentrations is first presented in this article. Both the linking style 1-4 and the arylation of the spacer with phenyl (Ph-) 5 or methoxyphenyl (MeOPh-) 6 substituents strongly affect the formation of luminophore aggregated forms in binary THF-water mixtures. The α,α-and β,β-isomers (1 and 3) form non-fluorescent H-type aggregates in THF-water mixtures with f<sub>w</sub> > 70%. The α,β-; β',β'-isomers (2, 4) and the MeOPh-substituted β,β-bichromophore 6 are characterized by predominant formation fluorescent aggregates. All bichromophores are characterized by the presence of residual amounts of non-aggregated forms in binary mixtures with maximum water content. The results are useful for controlling the aggregation behavior and spectral characteristics of CHR-bis(BODIPY) bichromophores in aqueous-organic media, which is important in the development of biomarkers and PDT agents.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The rapid and accurate detection of programmed death-ligand 1 (PD-L1) expression is of great value in the diagnosis and treatment of tumors. ELISA-based traditional method is the gold standard for protein detection, but there are still some shortcomings, especially the antigen-antibody dependence, greatly increased the detection time and cost. This work constructed a label-free fluorescent probe for rapid and sensitive detection of PD-L1 using a truncated aptamer as recognition molecules and double-stranded DNA specific dyes (SYBR Green I) as signal units. After a series of optimization conditions, this probe has good detection capability for PD-L1 in buffer solution with the detection limit as low as 0.68 ng/mL. Due to the specific recognition ability of aptamer and target, this method also has good selectivity for PD-L1 detection. The recovery of PD-L1 in human serum samples ranges from 86.20 to 96.36%. Compared with other methods, this strategy does not need to be marked, and does not need other complex design and purification process, but simple operation process and strong anti-interference ability. The whole detection process can be completed within 20 min and has good application prospect. This work will provide reference for drug dosage and prognosis evaluation of specific tumor therapy.
{"title":"Label-Free Detection of Programmed Death-Ligand 1 for Prognosis Using a Truncated Aptamer and SYBR Green I.","authors":"Ling Li, Zheng-Ling Li, Wei Wang, Xin-Xin Long, Ping Liu, Guo-Tian Wang, Shi-Qing Sun, Rong-Lian Du","doi":"10.1007/s10895-024-03960-x","DOIUrl":"https://doi.org/10.1007/s10895-024-03960-x","url":null,"abstract":"<p><p>The rapid and accurate detection of programmed death-ligand 1 (PD-L1) expression is of great value in the diagnosis and treatment of tumors. ELISA-based traditional method is the gold standard for protein detection, but there are still some shortcomings, especially the antigen-antibody dependence, greatly increased the detection time and cost. This work constructed a label-free fluorescent probe for rapid and sensitive detection of PD-L1 using a truncated aptamer as recognition molecules and double-stranded DNA specific dyes (SYBR Green I) as signal units. After a series of optimization conditions, this probe has good detection capability for PD-L1 in buffer solution with the detection limit as low as 0.68 ng/mL. Due to the specific recognition ability of aptamer and target, this method also has good selectivity for PD-L1 detection. The recovery of PD-L1 in human serum samples ranges from 86.20 to 96.36%. Compared with other methods, this strategy does not need to be marked, and does not need other complex design and purification process, but simple operation process and strong anti-interference ability. The whole detection process can be completed within 20 min and has good application prospect. This work will provide reference for drug dosage and prognosis evaluation of specific tumor therapy.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil-filled submarine cables have come to dominate the reliable delivery of electrical power today, but oil leakages still remain a challenge. This work focuses on exploring the detection of dodecylbenzene (DDB) after leakage. DDB can be dispersed as small droplets in seawater, which the diameter of DDB droplets increased from 60 nm to 200 nm with the increase of the concentration from 10 ppm to 1000 ppm. Moreover, excitation-emission matrix (EEM) spectra were employed to investigated the detection of DDB. DDB has characteristic fluorescence peaks located at λEx/λEm = 225/350 and 255/350. The FIo/w value of 50 ppm DDB was 28.869, which was significantly different from simulated seawater low as 1.3926. Contaminants such as metal ions (Cu2+, Cd2+, Zn2+) and organic matter (tetracycline) do not significantly affect the qualitative identification of DDB by EEMs. This study suggests a research basis for exploring the way for detection of DDB.
{"title":"Laboratory Investigation of Dodecylbenzene Detection in Seawater Via Fluorometric Index of Excitation-emission Matrix Spectra.","authors":"Jianping Liao, Zhifeng Liu, Weiqi Yang, Chen Tian, Xueyu Wu, Zhiping Zhu, Zhenggang Wang","doi":"10.1007/s10895-024-03978-1","DOIUrl":"https://doi.org/10.1007/s10895-024-03978-1","url":null,"abstract":"<p><p>Oil-filled submarine cables have come to dominate the reliable delivery of electrical power today, but oil leakages still remain a challenge. This work focuses on exploring the detection of dodecylbenzene (DDB) after leakage. DDB can be dispersed as small droplets in seawater, which the diameter of DDB droplets increased from 60 nm to 200 nm with the increase of the concentration from 10 ppm to 1000 ppm. Moreover, excitation-emission matrix (EEM) spectra were employed to investigated the detection of DDB. DDB has characteristic fluorescence peaks located at λ<sub>Ex</sub>/λ<sub>Em</sub> = 225/350 and 255/350. The FI<sub>o/w</sub> value of 50 ppm DDB was 28.869, which was significantly different from simulated seawater low as 1.3926. Contaminants such as metal ions (Cu<sup>2+</sup>, Cd<sup>2+</sup>, Zn<sup>2+</sup>) and organic matter (tetracycline) do not significantly affect the qualitative identification of DDB by EEMs. This study suggests a research basis for exploring the way for detection of DDB.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1007/s10895-024-03973-6
Anil Kumar, C G Renuka
Rotational dynamics of 3-(benzo[d]thiazol-2-yl)-7-(diethylamino)-2 H-chromen-2-one (3BT7D2H-one) probe in non-polar aprotic 1,4 dioxane, toluene and cyclohexane solvents of different and comparable viscosity are studied with varying temperature using steady-state and fluorescence depolarization technique. The experimental reorientation time is compared with the hydrodynamic Stoke's-Einstein-Debye(SED). The experimentally measured rotational reorientation times are higher in 1,4 dioxane attributed to its solvent viscosity. In cyclohexane, the solute rotational dynamics behavior shows a transition from sub-slip to SED slip boundary conditions with varying temperatures. Solvent viscosity, size, and subtle changes in the molecular shape of solute play an important role in the rotational dynamics of polar probes in non-polar aprotic solvents.
利用稳态和荧光去极化技术,研究了 3-(苯并[d]噻唑-2-基)-7-(二乙基氨基)-2 H-色烯-2-酮(3BT7D2H-one)探针在非极性的 1,4-二氧六环、甲苯和环己烷溶剂中的旋转动力学,这些溶剂的粘度不同且相当。实验重新定向时间与流体力学斯托克-爱因斯坦-德拜(SED)进行了比较。在 1,4 二氧六环中,由于溶剂粘度的原因,实验测得的旋转重新定向时间较长。在环己烷中,随着温度的变化,溶质旋转动力学行为显示了从亚滑移到 SED 滑动边界条件的过渡。溶剂粘度、溶质大小和分子形状的微妙变化对极性探针在非极性非质子溶剂中的旋转动力学起着重要作用。
{"title":"Temperature-Dependent Rotational Dynamics of a Polar Probe in Non-Polar Aprotic Solvents: Interplay of Solvent Viscosity and Size Effect.","authors":"Anil Kumar, C G Renuka","doi":"10.1007/s10895-024-03973-6","DOIUrl":"https://doi.org/10.1007/s10895-024-03973-6","url":null,"abstract":"<p><p>Rotational dynamics of 3-(benzo[d]thiazol-2-yl)-7-(diethylamino)-2 H-chromen-2-one (3BT7D2H-one) probe in non-polar aprotic 1,4 dioxane, toluene and cyclohexane solvents of different and comparable viscosity are studied with varying temperature using steady-state and fluorescence depolarization technique. The experimental reorientation time is compared with the hydrodynamic Stoke's-Einstein-Debye(SED). The experimentally measured rotational reorientation times are higher in 1,4 dioxane attributed to its solvent viscosity. In cyclohexane, the solute rotational dynamics behavior shows a transition from sub-slip to SED slip boundary conditions with varying temperatures. Solvent viscosity, size, and subtle changes in the molecular shape of solute play an important role in the rotational dynamics of polar probes in non-polar aprotic solvents.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nitroaromatic compounds are highly explosive and illegitimate substances. Over a decade, chemists have been affianced in extensive research on the selective and sensitive detection of these nitroaromatic explosives for homeland security and environmental protection. The benzodiazepine-based enaminone (BDE) receptor has been synthesized by aqueous extract of onion catalyzed three-component reaction between o-phenylenediamine, dimedone with an aldehyde. The BDE probe is well analyzed and applied to a sensor that selectively detects picric acid (PA). UV-Vis and fluorescence spectroscopy were used to investigate the photophysical responses of the receptor (BDE). From the observed results BDE found turn-off fluorescence with the addition of picric acid and the lowest limit of detection and limit of quantification was achieved about 24.6 nM and 73.8 nM. The fluorescence quantum yield was attained about 0.28. The BDE-PA adduct formation was confirmed by 1H NMR titration analysis. The Job's plot analysis was performed through 1H NMR titrations and established the binding stoichiometry ratio of the BDE-PA adduct as 1:1 ratio. Further, DFT calculations supported the observed photophysical responses of BDE-PA adduct to confirm the molecular level interactions. The receptor was effectively applied to approximate level of picric acid in real water sample analysis.
{"title":"Selective Detection of Picric Acid by Benzodiazepine Containing Enaminone Core as Receptor and Its Application to Real Water Sample Analysis.","authors":"Rajendran Eswaran, Loganathan Selvaraj, Santhiya Ramasamy, Sivaraman Gandhi, Seenivasa Perumal Muthu","doi":"10.1007/s10895-024-03925-0","DOIUrl":"https://doi.org/10.1007/s10895-024-03925-0","url":null,"abstract":"<p><p>Nitroaromatic compounds are highly explosive and illegitimate substances. Over a decade, chemists have been affianced in extensive research on the selective and sensitive detection of these nitroaromatic explosives for homeland security and environmental protection. The benzodiazepine-based enaminone (BDE) receptor has been synthesized by aqueous extract of onion catalyzed three-component reaction between o-phenylenediamine, dimedone with an aldehyde. The BDE probe is well analyzed and applied to a sensor that selectively detects picric acid (PA). UV-Vis and fluorescence spectroscopy were used to investigate the photophysical responses of the receptor (BDE). From the observed results BDE found turn-off fluorescence with the addition of picric acid and the lowest limit of detection and limit of quantification was achieved about 24.6 nM and 73.8 nM. The fluorescence quantum yield was attained about 0.28. The BDE-PA adduct formation was confirmed by <sup>1</sup>H NMR titration analysis. The Job's plot analysis was performed through <sup>1</sup>H NMR titrations and established the binding stoichiometry ratio of the BDE-PA adduct as 1:1 ratio. Further, DFT calculations supported the observed photophysical responses of BDE-PA adduct to confirm the molecular level interactions. The receptor was effectively applied to approximate level of picric acid in real water sample analysis.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}