首页 > 最新文献

Journal of fish diseases最新文献

英文 中文
AI-Driven Realtime Monitoring of Early Indicators for Ichthyophthirius multifiliis Infection of Rainbow Trout 人工智能驱动的虹鳟鱼多纤嗜鱼螺旋体感染早期指标实时监测。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-30 DOI: 10.1111/jfd.14027
Rikke Bonnichsen, Glenn Gunner Brink Nielsen, Jeppe Seidelin Dam, Dorte Schrøder-Petersen, Kurt Buchmann

A novel video-based real-time system based on AI (artificial intelligence) was developed to detect clinical signs in fish exposed to pathogens. We selected a White Spot Disease model involving rainbow trout as the experimental animal and the parasitic ciliate Ichthyophthirius multifiliis as a pathogen. We compared two identical fish tank systems: one tank was infected by co-habitation, whereas the other tank was kept non-infected (sham infection). The two fish tanks were separately video monitored (full top and side view) during the course of infection, during which fish were removed whenever they developed clinical signs (direct visual inspection by the observer). Image analysis (object detection, classification and tracking) was used to track behavioural changes in fish (in every recorded video frame), focusing on movement patterns and spatial localisation. Initially, the two fish groups (infected and non-infected) exhibited similar behaviour and non-infected fish did not change behaviour during the 15 d observation period (from 5 d before infection until 10 dpi). At 4, 7, 8, 9 and 10 dpi some infected fish showed clinical signs (equilibrium disturbance, gasping and lethargy) and were removed from the experiment. Anorexia occurred from 5 dpi and a gradual progression of gasping behaviour was noted, whereas the frequency of fish flashing (rubbing/scratching against objects) was low. Equilibrium disturbances and the development of white spots in the skin appeared to be a much later (8–10 dpi at this temperature) indicator of infection. The video analysis showed a general distribution of non-infected fish in all parts of the fish tank during the entire experiment, whereas infected fish already at 4–5 dpi moved towards higher water currents in the top and bottom positions. This change of fish positioning within the tank appeared as a promising early indicator of infection. The study suggests that continuous monitoring of fish behaviour using AI can potentially optimise the timing of humane endpoints, indicate disease signs earlier and thereby improve animal welfare in both animal experimentation and in aquaculture settings.

我们开发了一种基于 AI(人工智能)的新型视频实时系统,用于检测鱼类接触病原体后的临床症状。我们选择了一个白斑病模型,以虹鳟鱼为实验动物,以寄生纤毛虫Ichthyophthirius multifiliis为病原体。我们对两个相同的鱼缸系统进行了比较:一个鱼缸通过共栖感染了病原体,而另一个鱼缸则未感染病原体(假感染)。在感染过程中,分别对两个鱼缸进行视频监控(全俯视图和侧视图),在此期间,只要鱼出现临床症状(观察者直接目测),就将其移走。使用图像分析(物体检测、分类和跟踪)来跟踪鱼的行为变化(在每一帧录制的视频中),重点是运动模式和空间定位。最初,两组鱼(感染和未感染)表现出相似的行为,未感染的鱼在 15 天的观察期间(从感染前 5 天到 10 dpi)没有行为变化。在 4、7、8、9 和 10 dpi,一些受感染的鱼出现了临床症状(平衡失调、喘气和嗜睡),并被移出实验。厌食症从 5 dpi 开始出现,喘气行为逐渐加重,而鱼体闪光(摩擦/抓挠物体)的频率很低。平衡失调和皮肤出现白斑似乎是更晚些时候(在此温度下为 8-10 dpi)才出现的感染指标。视频分析表明,在整个实验过程中,未感染的鱼一般分布在鱼缸的各个部分,而在 4-5 dpi 时就已感染的鱼则向水流较高的顶部和底部位置移动。鱼在鱼缸中位置的这种变化似乎是一个很有希望的早期感染指标。这项研究表明,利用人工智能对鱼的行为进行连续监测,有可能优化人道终点的时间选择,提早显示疾病征兆,从而改善动物实验和水产养殖环境中的动物福利。
{"title":"AI-Driven Realtime Monitoring of Early Indicators for Ichthyophthirius multifiliis Infection of Rainbow Trout","authors":"Rikke Bonnichsen,&nbsp;Glenn Gunner Brink Nielsen,&nbsp;Jeppe Seidelin Dam,&nbsp;Dorte Schrøder-Petersen,&nbsp;Kurt Buchmann","doi":"10.1111/jfd.14027","DOIUrl":"10.1111/jfd.14027","url":null,"abstract":"<p>A novel video-based real-time system based on AI (artificial intelligence) was developed to detect clinical signs in fish exposed to pathogens. We selected a White Spot Disease model involving rainbow trout as the experimental animal and the parasitic ciliate <i>Ichthyophthirius multifiliis</i> as a pathogen. We compared two identical fish tank systems: one tank was infected by co-habitation, whereas the other tank was kept non-infected (sham infection). The two fish tanks were separately video monitored (full top and side view) during the course of infection, during which fish were removed whenever they developed clinical signs (direct visual inspection by the observer). Image analysis (object detection, classification and tracking) was used to track behavioural changes in fish (in every recorded video frame), focusing on movement patterns and spatial localisation. Initially, the two fish groups (infected and non-infected) exhibited similar behaviour and non-infected fish did not change behaviour during the 15 d observation period (from 5 d before infection until 10 dpi). At 4, 7, 8, 9 and 10 dpi some infected fish showed clinical signs (equilibrium disturbance, gasping and lethargy) and were removed from the experiment. Anorexia occurred from 5 dpi and a gradual progression of gasping behaviour was noted, whereas the frequency of fish flashing (rubbing/scratching against objects) was low. Equilibrium disturbances and the development of white spots in the skin appeared to be a much later (8–10 dpi at this temperature) indicator of infection. The video analysis showed a general distribution of non-infected fish in all parts of the fish tank during the entire experiment, whereas infected fish already at 4–5 dpi moved towards higher water currents in the top and bottom positions. This change of fish positioning within the tank appeared as a promising early indicator of infection. The study suggests that continuous monitoring of fish behaviour using AI can potentially optimise the timing of humane endpoints, indicate disease signs earlier and thereby improve animal welfare in both animal experimentation and in aquaculture settings.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of cardiac cell cultures from salmonids to measure the cardiotoxic effect of environmental pollutants 利用鲑鱼心脏细胞培养物测量环境污染物的心脏毒性效应。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-29 DOI: 10.1111/jfd.14018
Torben Krebs, Julia Bauer, Sarah Graff, Lukas Teich, Markus Sterneberg, Marina Gebert, Henrike Seibel, Bettina Seeger, Dieter Steinhagen, Verena Jung-Schroers, Mikolaj Adamek

Environmental stressors such as micro- and nanosized plastic particles (MNPs) or crude oil have a detrimental effect on aquatic animals; however, the impact upon the cardiovascular system of fish remains relatively under-researched. This study presents a novel approach for investigating the effect of crude oil and MNPs on the cardiac system of fish. We used salmonid larvae and cardiac cell cultures derived from hearts of salmonid fish and exposed them to environmental stressors. Following exposure to plastic particles or crude oil, the larvae exhibited some variation in contraction rate. In contrast, significant alterations in the contraction rate were observed in all cardiac cell cultures. The greatest differences between the control and treatment groups were observed in cardiac cell cultures derived from older brown trout. Following 7 days of exposure to MNPs or crude oil in Atlantic salmon larval hearts or cardiac cell cultures, there were only minor responses noted in mRNA expression of the selected marker genes. These findings show the use of a novel in vitro technique contributing to the existing body of knowledge on the impact of MNPs and crude oil on the cardiovascular system of salmonids and the associated risk.

微小和纳米塑料颗粒(MNPs)或原油等环境应激源会对水生动物产生有害影响,但对鱼类心血管系统影响的研究相对较少。本研究提出了一种研究原油和 MNPs 对鱼类心脏系统影响的新方法。我们使用鲑鱼幼体和来自鲑鱼心脏的心脏细胞培养物,并将它们暴露于环境应激源。暴露于塑料微粒或原油后,幼鱼的收缩率出现了一些变化。相反,在所有心脏细胞培养物中都观察到了收缩率的显著变化。对照组和处理组之间的最大差异出现在来自年长褐鳟鱼的心脏细胞培养物中。大西洋鲑幼鱼心脏或心脏细胞培养物接触 MNPs 或原油 7 天后,所选标记基因的 mRNA 表达仅出现轻微反应。这些研究结果表明,新型体外技术的使用有助于丰富关于 MNPs 和原油对鲑鱼心血管系统的影响及相关风险的现有知识。
{"title":"Use of cardiac cell cultures from salmonids to measure the cardiotoxic effect of environmental pollutants","authors":"Torben Krebs,&nbsp;Julia Bauer,&nbsp;Sarah Graff,&nbsp;Lukas Teich,&nbsp;Markus Sterneberg,&nbsp;Marina Gebert,&nbsp;Henrike Seibel,&nbsp;Bettina Seeger,&nbsp;Dieter Steinhagen,&nbsp;Verena Jung-Schroers,&nbsp;Mikolaj Adamek","doi":"10.1111/jfd.14018","DOIUrl":"10.1111/jfd.14018","url":null,"abstract":"<p>Environmental stressors such as micro- and nanosized plastic particles (MNPs) or crude oil have a detrimental effect on aquatic animals; however, the impact upon the cardiovascular system of fish remains relatively under-researched. This study presents a novel approach for investigating the effect of crude oil and MNPs on the cardiac system of fish. We used salmonid larvae and cardiac cell cultures derived from hearts of salmonid fish and exposed them to environmental stressors. Following exposure to plastic particles or crude oil, the larvae exhibited some variation in contraction rate. In contrast, significant alterations in the contraction rate were observed in all cardiac cell cultures. The greatest differences between the control and treatment groups were observed in cardiac cell cultures derived from older brown trout. Following 7 days of exposure to MNPs or crude oil in Atlantic salmon larval hearts or cardiac cell cultures, there were only minor responses noted in mRNA expression of the selected marker genes. These findings show the use of a novel in vitro technique contributing to the existing body of knowledge on the impact of MNPs and crude oil on the cardiovascular system of salmonids and the associated risk.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mucosal epithelial homeostasis: Reference intervals for skin, gill lamellae and filament for Atlantic salmon and other fish species 粘膜上皮细胞稳态:大西洋鲑鱼和其他鱼类的皮肤、鳃片和鱼丝的参考区间。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-24 DOI: 10.1111/jfd.14023
Grigory V. Merkin, Albert Girons, Mearge A. Okubamichael, Karin Pittman

Mucosal barriers are gatekeepers of health and exhibit homeostatic variation in relation to habitat and disease. Mucosal Mapping technology provides an in-depth examination of the dynamic mucous cells (MCs) in fish mucosal barriers on tangential sections, about 90° from the view of traditional histology. The method was originally developed and standardized in academia prior to the establishment of QuantiDoc AS to apply mucosal mapping, now trademarked as Veribarr™ for the analysis of skin, gills and gastrointestinal tracts. Veribarr™ uses design-based stereology for the selection and measurement of cell area (size) (μm2), the volumetric density of MCs in the epithelium (MCD, amount of the epithelia occupied by MCs, in %) and the calculated abundance of the MCs (barrier status or defence activity). MC production was mapped across the skin and gill epithelia in 12 species, discovering that gills consistently have two distinct groups of MCs, one on the lamellae where MCs are few and small and one on the filament where MCs are larger and more abundant. MCs were usually much larger in the skin than in the gills, with the latter requiring fewer and smaller cells for adequate respiration. The difference observed between MCs in gill lamella and gill filament is likely a result of functional demands. In addition, our findings also highlight a variation in the mucosal parameters between the species skin, which cannot be explained by the weight differences, and a potential link between MC distribution and species-specific lifestyles in the gill lamella. This diversity necessitates the development of species and tissue site-specific reference intervals for mucosal health evaluation. Mucosal bivariate reference intervals were developed for MC production, including size (trophy) and calculated defence activity (plasia) in the skin and gills of Atlantic salmon, to contrast new measurements against historical data patterns. The application of mucosal reference intervals demonstrates that stress from parasites and treatments can manifest as changes in mucosal architecture, as evidenced by MC hypertrophy and hyperplasia within the gill lamellae. These reference intervals also facilitate comparisons with wild Atlantic salmon, revealing a somewhat higher MC level in farmed salmon gill lamellae. These findings suggest that MC hyperplasia and hypertrophy in the gills are stress/environmental responses in aquaculture. They also advocate for developing specific mucosal bivariate homeostatic reference intervals in aquaculture to improve fish health and welfare across all farmed species.

粘膜屏障是鱼类健康的守护者,会因栖息地和疾病的不同而发生同态变化。粘膜映射技术可在切线切片上深入检查鱼类粘膜屏障中的动态粘液细胞(MC),与传统组织学的视角约成 90°。在 QuantiDoc AS 成立之前,该方法最初是在学术界开发并标准化的,用于皮肤、鳃和胃肠道分析的粘膜映射技术现已注册为 Veribarr™。Veribarr™ 采用基于设计的立体学方法来选择和测量细胞面积(大小)(μm2)、上皮细胞中 MC 的体积密度(MCD,MC 占上皮细胞的百分比)以及计算出的 MC 丰度(屏障状态或防御活动)。我们对 12 个物种的皮肤和鳃上皮细胞的 MC 产量进行了绘制,发现鳃上始终有两组不同的 MC,一组位于薄片上,MC 数量少且小,另一组位于丝状上,MC 数量多且大。皮肤上的 MC 通常比鳃上的大得多,后者需要更少、更小的细胞来进行充分的呼吸。在鳃片和鳃丝中观察到的 MCs 之间的差异可能是功能需求的结果。此外,我们的研究结果还突显了不同物种皮肤粘膜参数的差异(这不能用体重差异来解释),以及 MC 的分布与鳃盖中物种特有的生活方式之间的潜在联系。这种多样性要求为粘膜健康评估制定特定物种和组织部位的参考区间。为大西洋鲑鱼皮肤和鳃中的 MC 产量(包括体型(trophy)和计算出的防御活动(plasia))制定了粘膜双变量参考区间,以便将新的测量结果与历史数据模式进行对比。粘膜参考区间的应用表明,寄生虫和治疗造成的压力可表现为粘膜结构的变化,鳃层内的MC肥大和增生就是证明。这些参考区间还有助于与野生大西洋鲑进行比较,显示养殖鲑鱼鳃片的 MC 水平略高。这些发现表明,鳃中 MC 的增生和肥大是水产养殖中的应激/环境反应。他们还主张在水产养殖中开发特定的粘膜双变量同态参考区间,以改善所有养殖物种的鱼类健康和福利。
{"title":"Mucosal epithelial homeostasis: Reference intervals for skin, gill lamellae and filament for Atlantic salmon and other fish species","authors":"Grigory V. Merkin,&nbsp;Albert Girons,&nbsp;Mearge A. Okubamichael,&nbsp;Karin Pittman","doi":"10.1111/jfd.14023","DOIUrl":"10.1111/jfd.14023","url":null,"abstract":"<p>Mucosal barriers are gatekeepers of health and exhibit homeostatic variation in relation to habitat and disease. Mucosal Mapping technology provides an in-depth examination of the dynamic mucous cells (MCs) in fish mucosal barriers on tangential sections, about 90° from the view of traditional histology. The method was originally developed and standardized in academia prior to the establishment of QuantiDoc AS to apply mucosal mapping, now trademarked as Veribarr™ for the analysis of skin, gills and gastrointestinal tracts. Veribarr™ uses design-based stereology for the selection and measurement of cell area (size) (μm<sup>2</sup>), the volumetric density of MCs in the epithelium (MCD, amount of the epithelia occupied by MCs, in %) and the calculated abundance of the MCs (barrier status or defence activity). MC production was mapped across the skin and gill epithelia in 12 species, discovering that gills consistently have two distinct groups of MCs, one on the lamellae where MCs are few and small and one on the filament where MCs are larger and more abundant. MCs were usually much larger in the skin than in the gills, with the latter requiring fewer and smaller cells for adequate respiration. The difference observed between MCs in gill lamella and gill filament is likely a result of functional demands. In addition, our findings also highlight a variation in the mucosal parameters between the species skin, which cannot be explained by the weight differences, and a potential link between MC distribution and species-specific lifestyles in the gill lamella. This diversity necessitates the development of species and tissue site-specific reference intervals for mucosal health evaluation. Mucosal bivariate reference intervals were developed for MC production, including size (trophy) and calculated defence activity (plasia) in the skin and gills of Atlantic salmon, to contrast new measurements against historical data patterns. The application of mucosal reference intervals demonstrates that stress from parasites and treatments can manifest as changes in mucosal architecture, as evidenced by MC hypertrophy and hyperplasia within the gill lamellae. These reference intervals also facilitate comparisons with wild Atlantic salmon, revealing a somewhat higher MC level in farmed salmon gill lamellae. These findings suggest that MC hyperplasia and hypertrophy in the gills are stress/environmental responses in aquaculture. They also advocate for developing specific mucosal bivariate homeostatic reference intervals in aquaculture to improve fish health and welfare across all farmed species.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"48 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature-dependent alterations in the proteome of the emergent fish pathogen Edwardsiella piscicida. 新出现的鱼类病原体 Edwardsiella piscicida 蛋白质体随温度的变化。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-20 DOI: 10.1111/jfd.14017
Kim L Jacobsen, Matt Griffin, Brett S Phinney, Michelle Salemi, Zeinab Yazdi, Sujita Balami, Caitlin E Older, Esteban Soto

Edwardsiella piscicida is an emerging bacterial pathogen and the aetiological agent of edwardsiellosis among cultured and wild fish species globally. The increased frequency of outbreaks of this Gram-negative, facultative intracellular pathogen pose not only a threat to the aquaculture industry but also a possible foodborne/waterborne public health risk due to the ill-defined zoonotic potential. Thus, understanding the role of temperature on the virulence of this emerging pathogen is essential for comprehending the pathogenesis of piscine edwardsiellosis in the context of current warming trends associated with climate change, as well as providing insight into its zoonotic potential. In this study, significant temperature-dependent alterations in bacterial growth patterns were observed, with bacterial isolates grown at 17°C displaying higher peak growth sizes, extended lag times, and slower maximal growth rates than isolates grown at 27or 37°C. When E. piscicida isolates were grown at 37°C compared to 27 and 17°C, mass spectrometry analysis of the E. piscicida proteome revealed significant downregulation of crucial virulence proteins, such as Type VI secretion system proteins and flagellar proteins. Although in vivo models of infection are warranted, this in vitro data suggests possible temperature-associated alterations in the virulence and pathogenic potential of E. piscicida in poikilotherms and homeotherms.

鱼类爱德华氏菌(Edwardsiella piscicida)是一种新出现的细菌病原体,也是全球养殖鱼类和野生鱼类爱德华氏菌病的病原体。这种革兰氏阴性、兼性细胞内病原体的爆发频率越来越高,不仅对水产养殖业构成威胁,而且由于其人畜共患病的可能性尚不明确,还可能带来食源性/水传播的公共卫生风险。因此,了解温度对这一新兴病原体毒力的作用,对于在当前与气候变化相关的气候变暖趋势下理解鱼类爱德华氏菌病的致病机理以及深入了解其人畜共患病的可能性至关重要。在这项研究中,观察到细菌的生长模式发生了明显的温度依赖性变化,与在 27 或 37 摄氏度条件下生长的细菌相比,在 17 摄氏度条件下生长的细菌分离物显示出更高的生长峰值、更长的滞后时间和更慢的最大生长速率。与 27 和 17 摄氏度相比,在 37 摄氏度下生长的鱼腥鸥分离物的鱼腥鸥蛋白质组的质谱分析表明,关键的毒力蛋白(如 VI 型分泌系统蛋白和鞭毛蛋白)显著下调。虽然还需要建立体内感染模型,但这些体外数据表明,鱼腥藻的毒力和致病潜力可能会随着温度的变化而改变。
{"title":"Temperature-dependent alterations in the proteome of the emergent fish pathogen Edwardsiella piscicida.","authors":"Kim L Jacobsen, Matt Griffin, Brett S Phinney, Michelle Salemi, Zeinab Yazdi, Sujita Balami, Caitlin E Older, Esteban Soto","doi":"10.1111/jfd.14017","DOIUrl":"10.1111/jfd.14017","url":null,"abstract":"<p><p>Edwardsiella piscicida is an emerging bacterial pathogen and the aetiological agent of edwardsiellosis among cultured and wild fish species globally. The increased frequency of outbreaks of this Gram-negative, facultative intracellular pathogen pose not only a threat to the aquaculture industry but also a possible foodborne/waterborne public health risk due to the ill-defined zoonotic potential. Thus, understanding the role of temperature on the virulence of this emerging pathogen is essential for comprehending the pathogenesis of piscine edwardsiellosis in the context of current warming trends associated with climate change, as well as providing insight into its zoonotic potential. In this study, significant temperature-dependent alterations in bacterial growth patterns were observed, with bacterial isolates grown at 17°C displaying higher peak growth sizes, extended lag times, and slower maximal growth rates than isolates grown at 27or 37°C. When E. piscicida isolates were grown at 37°C compared to 27 and 17°C, mass spectrometry analysis of the E. piscicida proteome revealed significant downregulation of crucial virulence proteins, such as Type VI secretion system proteins and flagellar proteins. Although in vivo models of infection are warranted, this in vitro data suggests possible temperature-associated alterations in the virulence and pathogenic potential of E. piscicida in poikilotherms and homeotherms.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":" ","pages":"e14017"},"PeriodicalIF":2.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization of publicly available data to summarize spatio-temporal patterns of fish health events of Atlantic salmon (Salmo salar) reported by marine finfish industries in British Columbia (BC), Canada 利用公开数据总结加拿大不列颠哥伦比亚省(BC)海洋鱼类产业报告的大西洋鲑鱼(Salmo salar)鱼类健康事件的时空模式
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-18 DOI: 10.1111/jfd.14022
Sumit Jyoti, Beibei Jia, Sonja Saksida, Henrik Stryhn, Derek Price, Krishna Kumar Thakur

Atlantic salmon aquaculture companies in British Columbia (BC) must report fish health events to Fisheries and Oceans Canada (DFO) as part of their licensing conditions. Our study aimed to summarize these fish health events reported by Atlantic salmon sites in BC to identify spatial and spatio-temporal clusters. We conducted descriptive, retrospective global, and local cluster analyses using Moran's I and scan statistics. Between 2016 and 2022, 265 fish health events were reported. The annual incidence ranged from 5.60 (95% CI: 3.90–7.80) to 6.86 (95% CI: 4.70–9.60) health events per 100 active site-months. The most common events were yellow mouth (60.75%; 161/265) and salmonid rickettsial septicaemia (SRS) (15.47%; 41/265). The Moran's I index was positive and significant for yellow mouth, SRS, and overall fish health events at different distance bands. Most of the spatial and spatio-temporal clusters were identified in the west-central and southwestern parts of Vancouver Island. Our study hypothesizes that management practices, environmental conditions, and water quality parameters may have influenced the increased reporting of fish health events in these regions. Overall, the study highlights the potential of publicly available data for practical risk mapping in understanding the patterns of farmed Atlantic salmon diseases in BC.

作为许可条件的一部分,不列颠哥伦比亚省(BC)的大西洋鲑水产养殖公司必须向加拿大渔业及海洋部(DFO)报告鱼类健康事件。我们的研究旨在总结不列颠哥伦比亚省大西洋鲑养殖场报告的这些鱼类健康事件,以确定空间和时空集群。我们使用莫兰 I 统计法和扫描统计法进行了描述性、回顾性全球和局部聚类分析。2016 年至 2022 年期间,共报告了 265 起鱼类健康事件。每年每 100 个活动地点月发生 5.60(95% CI:3.90-7.80)至 6.86(95% CI:4.70-9.60)起健康事件。最常见的事件是黄口病(60.75%;161/265)和鲑鱼立克次体败血症(SRS)(15.47%;41/265)。不同距离带的黄口病、SRS 和总体鱼类健康事件的 Moran's I 指数为正且显著。在温哥华岛的中西部和西南部发现了大多数空间和时空集群。我们的研究假设,管理方法、环境条件和水质参数可能影响了这些地区鱼类健康事件报告的增加。总之,这项研究强调了公开数据在绘制实用风险图以了解不列颠哥伦比亚省养殖大西洋鲑疾病模式方面的潜力。
{"title":"Utilization of publicly available data to summarize spatio-temporal patterns of fish health events of Atlantic salmon (Salmo salar) reported by marine finfish industries in British Columbia (BC), Canada","authors":"Sumit Jyoti,&nbsp;Beibei Jia,&nbsp;Sonja Saksida,&nbsp;Henrik Stryhn,&nbsp;Derek Price,&nbsp;Krishna Kumar Thakur","doi":"10.1111/jfd.14022","DOIUrl":"10.1111/jfd.14022","url":null,"abstract":"<p>Atlantic salmon aquaculture companies in British Columbia (BC) must report fish health events to Fisheries and Oceans Canada (DFO) as part of their licensing conditions. Our study aimed to summarize these fish health events reported by Atlantic salmon sites in BC to identify spatial and spatio-temporal clusters. We conducted descriptive, retrospective global, and local cluster analyses using Moran's <i>I</i> and scan statistics. Between 2016 and 2022, 265 fish health events were reported. The annual incidence ranged from 5.60 (95% CI: 3.90–7.80) to 6.86 (95% CI: 4.70–9.60) health events per 100 active site-months. The most common events were yellow mouth (60.75%; 161/265) and salmonid rickettsial septicaemia (SRS) (15.47%; 41/265). The Moran's I index was positive and significant for yellow mouth, SRS, and overall fish health events at different distance bands. Most of the spatial and spatio-temporal clusters were identified in the west-central and southwestern parts of Vancouver Island. Our study hypothesizes that management practices, environmental conditions, and water quality parameters may have influenced the increased reporting of fish health events in these regions. Overall, the study highlights the potential of publicly available data for practical risk mapping in understanding the patterns of farmed Atlantic salmon diseases in BC.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 12","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A droplet digital PCR assay to detect Chinese rice-field eels rhabdovirus 检测中国稻田黄鳝横纹肌病毒的液滴数字 PCR 法。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-16 DOI: 10.1111/jfd.14020
Wenzhi Liu, Yong Zhou, Nan Jiang, Chen Xu, Qiwang Zhong, Yuding Fan

Chinese rice-field eels rhabdovirus (CrERV) causes haemorrhagic disease in Chinese rice-field eels (Monopterus albus), leading to significant mortality and economic losses. Sensitive detection of CrERV nucleic acids is essential to control the spread of this pathogen and to treat infected individuals. Herein, we developed an efficient and sensitive droplet digital PCR (ddPCR) method to rapidly detect and quantify CrERV. The ddPCR assay optimal conditions were an annealing temperature of 53°C, and primer and probe concentrations of 0.5 and 0.25 μM, respectively. The assay had a diagnostic sensitivity of 0.23 copies/μL, and was highly specific, showing no cross reactivity with other viruses (infectious haematopoietic necrosis virus, grass carp reovirus, spring viremia of carp virus, largemouth bass ranavirus, carp edema virus, Chinese giant salamander iridovirus, and white spot syndrome virus). Real-time quantitative PCR testing of 30 Chinese rice-field eels samples detected CrERV in 7 samples (23.3%), whereas ddPCR detected CrERV in 12 samples (40%), demonstrating its higher sensitivity. Thus, ddPCR represents an advanced method to absolutely quantify CrERV in infected fish with low virus concentrations, providing a valuable tool to manage the spread and impact of CrERV.

中国稻田黄鳝横纹肌病毒(CrERV)会导致中国稻田黄鳝(Monopterus albus)出血性疾病,造成大量死亡和经济损失。对 CrERV 核酸的灵敏检测对于控制该病原体的传播和治疗感染者至关重要。在此,我们开发了一种高效、灵敏的液滴数字 PCR(ddPCR)方法来快速检测和量化 CrERV。ddPCR 检测的最佳条件是退火温度为 53°C,引物和探针的浓度分别为 0.5 和 0.25 μM。该检测方法的诊断灵敏度为 0.23 拷贝/μL,特异性高,与其他病毒(传染性造血坏死病毒、草鱼再病毒、鲤鱼春季病毒血症、大口鲈鱼匐行病毒、鲤鱼水肿病毒、中国大鲵虹彩病毒和白斑综合征病毒)无交叉反应。在对 30 份中国稻田黄鳝样本进行实时定量 PCR 检测时,有 7 份样本(23.3%)检测出 CrERV,而 ddPCR 检测有 12 份样本(40%)检测出 CrERV,这表明 ddPCR 的灵敏度更高。因此,ddPCR 是一种先进的方法,可在病毒浓度较低的感染鱼体内绝对量化 CrERV,为控制 CrERV 的传播和影响提供了宝贵的工具。
{"title":"A droplet digital PCR assay to detect Chinese rice-field eels rhabdovirus","authors":"Wenzhi Liu,&nbsp;Yong Zhou,&nbsp;Nan Jiang,&nbsp;Chen Xu,&nbsp;Qiwang Zhong,&nbsp;Yuding Fan","doi":"10.1111/jfd.14020","DOIUrl":"10.1111/jfd.14020","url":null,"abstract":"<p>Chinese rice-field eels rhabdovirus (CrERV) causes haemorrhagic disease in Chinese rice-field eels (<i>Monopterus albus</i>), leading to significant mortality and economic losses. Sensitive detection of CrERV nucleic acids is essential to control the spread of this pathogen and to treat infected individuals. Herein, we developed an efficient and sensitive droplet digital PCR (ddPCR) method to rapidly detect and quantify CrERV. The ddPCR assay optimal conditions were an annealing temperature of 53°C, and primer and probe concentrations of 0.5 and 0.25 μM, respectively. The assay had a diagnostic sensitivity of 0.23 copies/μL, and was highly specific, showing no cross reactivity with other viruses (infectious haematopoietic necrosis virus, grass carp reovirus, spring viremia of carp virus, largemouth bass ranavirus, carp edema virus, Chinese giant salamander iridovirus, and white spot syndrome virus). Real-time quantitative PCR testing of 30 Chinese rice-field eels samples detected CrERV in 7 samples (23.3%), whereas ddPCR detected CrERV in 12 samples (40%), demonstrating its higher sensitivity. Thus, ddPCR represents an advanced method to absolutely quantify CrERV in infected fish with low virus concentrations, providing a valuable tool to manage the spread and impact of CrERV.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 12","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spontaneous exophytic tumour in Nothobranchius furzeri, an aging research model 老化研究模型 Nothobranchius furzeri 的自发性外生肿瘤。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-16 DOI: 10.1111/jfd.14021
Jakub Žák, Ľubomír Pojezdal, Iva Dyková
<p>Understanding the nature and diversity of spontaneous neoplasias in a model organism is of peculiar interest to toxicologists and biogerontologists who assess their prevalence in relation to experimental treatments (Wolf et al., <span>2015</span>). In fishes, exophytic neoplasias are frequently found due to their conspicuous appearance on the body (Roberts, <span>2012</span>; Vergneau-Grosset et al., <span>2017</span>). Papillomas are the most common exophytic epithelial fish tumours with a predominant proliferation of epidermal cells with no to little presence of connective tissue (Roberts, <span>2012</span>). Other exophytic tumours are derived from connective tissue as in fibromas, myxomas and fibromyxomas. Myxomas are soft tumours formed by immature connective tissue with extensive myxoid stroma. In contrast, a fibroma is a firm tumour derived from fibrous connective tissue (Roberts, <span>2012</span>). Fibromyxomas share some traits of myxoma and fibroma and commonly consist of proliferating connective tissue characterized by neoplastic growth of stellate and spindled cells within the loose, collagenous and myxoid matrix with a low number of mitotic figures (Manera et al., <span>1997</span>; Pittman & Montgomery, <span>2015</span>; Vergneau-Grosset et al., <span>2017</span>). They are typically well-vascularized and are connected to the subcutaneous musculature (Mocellin, <span>2021</span>; Song et al., <span>2016</span>). The prevalence of neoplasia in teleost fishes, including laboratory model organisms, is disproportionally biased due to frequent confusion with pathogen-induced lesions (Dyková et al., <span>2021</span>; Harshbarger, <span>1984</span>). Therefore, it is always important to test for the presence of known pathogens in the examined animal with neoplasia.</p><p>Here, we report a case of spontaneous fibromyxoma, in senescent laboratory-kept turquoise killifish <i>Nothobranchius furzeri</i>. Turquoise killifish are a rapidly aging species with a life expectancy of several months in the wild because they inhabit seasonally inundated pools in Southeast African savanna (Reichard & Polačik, <span>2018</span>). In captivity, they live approximately 1 year; therefore, they have become a popular model organism in biogerontology and toxicology (Cellerino et al., <span>2016</span>; Thoré et al., <span>2021</span>).</p><p>A male 12.5 months old <i>Nothobranchius furzeri</i> (50 mm SL, strain MZCS 222; Cellerino et al., <span>2016</span>) was examined. The fish was housed individually in a 2 L tank in a recirculation system (water temperature 27.5°C, water conductivity 1.5 mS × cm<sup>−2</sup>) with 188 other fish of the same age at an accredited (12,210/2018-MZE-17214) breeding facility of the Institute of Vertebrate Biology, Czechia. The fish was euthanized by an overdose of clove oil when it was unable to dive (a common symptom in senescent <i>N. furzeri</i>; Naumann et al., <span>2023</span>). The tumour tissue sample (5 × 8
我们的观察结果与 Tinca tinca(Manera &amp; Biavati,1995 年)和鲻鱼 Mugil cephalus(Lopez &amp; Raibaut,1981 年)的研究结果非常吻合。在增生的结缔组织中存在表皮细胞小体,这使我们的纤维瘤有别于之前的报告(Singaravel 等人,2016 年;Stevens 等人,2017 年)。肿瘤性结缔组织由于生长迅速,可以包裹其他类型的细胞,鲻鱼纤维瘤中的色素细胞也是如此(Lopez &amp; Raibaut, 1981)。我们推测,增殖的表皮细胞逐渐覆盖结缔组织,在肌胶原基质不断增殖的过程中,表皮细胞最终进入瘤组织内部。据我们所知,这是第一份关于诺氏鲈鱼外生性肿瘤的报告。据我们所知,这是第一份关于诺氏鳞鳉外生肿瘤的报告。在脊椎动物生物学研究所 15 年的诺氏鳞鳉养殖史上,以及 10 次野外采样活动中,从未观察到过这种肿瘤。与之前的假设(Baumgart等人,2015年;Blažek等人,2017年;Di Cicco等人,2011年)相反,即使是在高龄期,自发性肿瘤在Nothobranchius鱼类中也很罕见(Dyková等人,2021年),只有在高龄期才会出现肿瘤、Dyková等人,2020;Markofsky &amp; Milstoc, 1979a, 1979b; Stolk, 1959)。形态学诊断、内脏器官的正常外观(感染时会改变颜色或大小)以及病原体的分子、培养和染色诊断方法的阴性结果都证明了本报告中的肿瘤是自发性的。同样,由于持续的健康监测和无害的饲养条件,环境造成伤害的可能性也不大。12.5 个月的寿命是该物种中最长的寿命之一,20%的鱼群能存活到这个年龄(同时哨兵鱼体内的病原体检测结果为阴性),这表明鱼群中没有背景感染。这里报告的肿瘤是罕见的,但我们不能排除它可能与接触化学物质有关,如其他鱼类的各种肿瘤(如 Chen 等人,1996 年)。将自发性病变作为对比材料进行报告对毒理学家来说特别有意义。肿瘤没有影响标本的游泳和进食能力,这与其他纤维瘤的报告一致(Shokrpoor 等人,2017 年)。肿瘤在生长过程中,由光滑的椭圆形变为更不规则的形状,表面出现小溃疡。溃疡在大型肿瘤中很常见(Manera &amp; Biavati, 1995; Singaravel et al.)与其他报告(Manera &amp; Biavati,1995 年;Shokrpoor 等人,2017 年)一样,该纤维瘤是良性的。人工饲养的生物超过了它们在野外的自然寿命,更容易发生肿瘤(Hochberg &amp; Noble, 2017)。这一点已在小鼠身上得到证实(Gardner 等人,1973 年;Pompei 等人,2001 年)。在这里,当鱼出现衰老表型时,纤维肌瘤开始出现在鱼的高龄阶段,相当于野生N. furzeri最大寿命的三倍(Žák等人,2021年)。被诊断出的骨骼肌萎缩可能是衰老的症状,而且鱼体有(可能与衰老有关的)低体况迹象,包括肝脏空泡化缺乏和内脏脂肪储存不足。迄今为止,Nothobranchius属中无可争议的自发性肿瘤已在高龄时被发现(如Dyková等人,2020年;Markofsky &amp; Milstoc, 1979a, 1979b),但并不像以前假设的那样频繁(如Blažek等人,2017年)、人类的纤维肉瘤通常通过免疫组化方法进行诊断(Mocellin,2021;Pittman &amp; Montgomery,2015;Song 等人,2016)。我们没有采用免疫组化分析,因为这种方法很少适用于远洋鱼类的肿瘤,目前也无法与哺乳动物的研究结果进行比较(Groff,2004;Vergneau-Grosset 等人,2017)。另一方面,肿瘤的显微解剖外观明确支持我们对纤维瘤的诊断。据我们所知,本报告描述了第一个已知的绿松石鳉鱼外生纤维瘤的实例。我们认为肿瘤的形成是由于衰老,因为它是在高龄时出现的,而且与病原体无关。这些发现提供了新的证据,证明以前怀疑容易发生肿瘤的物种--毛鳞鱼(Nothobranchius furzeri)发生了自发性肿瘤:JZ;方法、验证、形式分析、调查、资源:JZ、ID、LP;数据整理:撰写-初稿:JZ、ID;撰写-审稿:LP:JZ、ID;写作-审阅和编辑:JZ、ID、LP;项目管理:JZ、ID。
{"title":"Spontaneous exophytic tumour in Nothobranchius furzeri, an aging research model","authors":"Jakub Žák,&nbsp;Ľubomír Pojezdal,&nbsp;Iva Dyková","doi":"10.1111/jfd.14021","DOIUrl":"10.1111/jfd.14021","url":null,"abstract":"&lt;p&gt;Understanding the nature and diversity of spontaneous neoplasias in a model organism is of peculiar interest to toxicologists and biogerontologists who assess their prevalence in relation to experimental treatments (Wolf et al., &lt;span&gt;2015&lt;/span&gt;). In fishes, exophytic neoplasias are frequently found due to their conspicuous appearance on the body (Roberts, &lt;span&gt;2012&lt;/span&gt;; Vergneau-Grosset et al., &lt;span&gt;2017&lt;/span&gt;). Papillomas are the most common exophytic epithelial fish tumours with a predominant proliferation of epidermal cells with no to little presence of connective tissue (Roberts, &lt;span&gt;2012&lt;/span&gt;). Other exophytic tumours are derived from connective tissue as in fibromas, myxomas and fibromyxomas. Myxomas are soft tumours formed by immature connective tissue with extensive myxoid stroma. In contrast, a fibroma is a firm tumour derived from fibrous connective tissue (Roberts, &lt;span&gt;2012&lt;/span&gt;). Fibromyxomas share some traits of myxoma and fibroma and commonly consist of proliferating connective tissue characterized by neoplastic growth of stellate and spindled cells within the loose, collagenous and myxoid matrix with a low number of mitotic figures (Manera et al., &lt;span&gt;1997&lt;/span&gt;; Pittman &amp; Montgomery, &lt;span&gt;2015&lt;/span&gt;; Vergneau-Grosset et al., &lt;span&gt;2017&lt;/span&gt;). They are typically well-vascularized and are connected to the subcutaneous musculature (Mocellin, &lt;span&gt;2021&lt;/span&gt;; Song et al., &lt;span&gt;2016&lt;/span&gt;). The prevalence of neoplasia in teleost fishes, including laboratory model organisms, is disproportionally biased due to frequent confusion with pathogen-induced lesions (Dyková et al., &lt;span&gt;2021&lt;/span&gt;; Harshbarger, &lt;span&gt;1984&lt;/span&gt;). Therefore, it is always important to test for the presence of known pathogens in the examined animal with neoplasia.&lt;/p&gt;&lt;p&gt;Here, we report a case of spontaneous fibromyxoma, in senescent laboratory-kept turquoise killifish &lt;i&gt;Nothobranchius furzeri&lt;/i&gt;. Turquoise killifish are a rapidly aging species with a life expectancy of several months in the wild because they inhabit seasonally inundated pools in Southeast African savanna (Reichard &amp; Polačik, &lt;span&gt;2018&lt;/span&gt;). In captivity, they live approximately 1 year; therefore, they have become a popular model organism in biogerontology and toxicology (Cellerino et al., &lt;span&gt;2016&lt;/span&gt;; Thoré et al., &lt;span&gt;2021&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;A male 12.5 months old &lt;i&gt;Nothobranchius furzeri&lt;/i&gt; (50 mm SL, strain MZCS 222; Cellerino et al., &lt;span&gt;2016&lt;/span&gt;) was examined. The fish was housed individually in a 2 L tank in a recirculation system (water temperature 27.5°C, water conductivity 1.5 mS × cm&lt;sup&gt;−2&lt;/sup&gt;) with 188 other fish of the same age at an accredited (12,210/2018-MZE-17214) breeding facility of the Institute of Vertebrate Biology, Czechia. The fish was euthanized by an overdose of clove oil when it was unable to dive (a common symptom in senescent &lt;i&gt;N. furzeri&lt;/i&gt;; Naumann et al., &lt;span&gt;2023&lt;/span&gt;). The tumour tissue sample (5 × 8","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 12","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jfd.14021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Droplet digital PCR for fish pathogen detection and quantification: A systematic review and meta-analysis 用于鱼类病原体检测和定量的液滴数字 PCR:系统综述和荟萃分析。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-16 DOI: 10.1111/jfd.14019
Md Afsar Ahmed Sumon, Edinson Dante Meregildo-Rodriguez, Po-Tsang Lee, Nguyen Dinh-Hung, Earl T. Larson, Patima Permpoonpattana, Hien Van Doan, Won-Kyo Jung, Nguyen Vu Linh

This study provides a comprehensive summary of the findings regarding the application and diagnostic efficacy of droplet digital PCR (ddPCR) in detecting viral and bacterial pathogens in aquaculture. Utilizing a systematic search of four databases up to 6 November 2023, we identified studies where ddPCR was deployed for pathogen detection in aquaculture settings, adhering to Preferred Reporting Items for Systematic Reviews and Meta-analysis of Diagnostic Test Accuracy guidelines. From the collected data, 16 studies retrieved, seven were included in a meta-analysis, encompassing 1121 biological samples from various fish species. The detection limits reported ranged markedly from 0.07 to 34 copies/μL. A direct comparison of the diagnostic performance between ddPCR with quantitative PCR (qPCR) proved challenging due to limited data, thus only a pooled sensitivity analysis was feasible. The results showed a pooled sensitivity of 0.750 (95% confidence interval [CI]: 0.487–0.944) for ddPCR, compared to 0.461 (95% CI: 0.294–0.632) for qPCR, with no statistically significant difference in sensitivity between the two methods (p = .5884). Notably, significant heterogeneity was observed among the studies (I2 = 93%–97%, p < .01), with the year of publication significantly influencing this heterogeneity (p < .001), but not the country of origin (p = .49). No publication bias was detected, and the studies generally exhibited a low risk of bias according to QUADAS-C criteria. While ddPCR and qPCR showed comparable sensitivities in pathogen detection, ddPCR's capability to precisely quantify pathogens without the need for standard curves highlights its potential utility. This characteristic could significantly enhance the accuracy and reliability of pathogen detection in aquaculture.

本研究全面总结了有关液滴数字 PCR(ddPCR)在水产养殖中检测病毒和细菌病原体的应用和诊断效果的研究结果。通过对截至 2023 年 11 月 6 日的四个数据库进行系统检索,我们确定了在水产养殖环境中应用 ddPCR 检测病原体的研究,并遵循了诊断测试准确性系统综述和荟萃分析首选报告项目指南。从收集到的数据中,检索到 16 项研究,其中 7 项被纳入荟萃分析,涵盖来自不同鱼类物种的 1121 份生物样本。报告的检测限明显不同,从 0.07 到 34 个拷贝/微升不等。由于数据有限,直接比较 ddPCR 和定量 PCR (qPCR) 的诊断性能具有挑战性,因此只能进行集合灵敏度分析。结果显示,ddPCR 的集合灵敏度为 0.750(95% 置信区间 [CI]:0.487-0.944),而 qPCR 为 0.461(95% 置信区间 [CI]:0.294-0.632),两种方法的灵敏度差异无统计学意义(p = .5884)。值得注意的是,研究之间存在明显的异质性(I2 = 93%-97%,p
{"title":"Droplet digital PCR for fish pathogen detection and quantification: A systematic review and meta-analysis","authors":"Md Afsar Ahmed Sumon,&nbsp;Edinson Dante Meregildo-Rodriguez,&nbsp;Po-Tsang Lee,&nbsp;Nguyen Dinh-Hung,&nbsp;Earl T. Larson,&nbsp;Patima Permpoonpattana,&nbsp;Hien Van Doan,&nbsp;Won-Kyo Jung,&nbsp;Nguyen Vu Linh","doi":"10.1111/jfd.14019","DOIUrl":"10.1111/jfd.14019","url":null,"abstract":"<p>This study provides a comprehensive summary of the findings regarding the application and diagnostic efficacy of droplet digital PCR (ddPCR) in detecting viral and bacterial pathogens in aquaculture. Utilizing a systematic search of four databases up to 6 November 2023, we identified studies where ddPCR was deployed for pathogen detection in aquaculture settings, adhering to Preferred Reporting Items for Systematic Reviews and Meta-analysis of Diagnostic Test Accuracy guidelines. From the collected data, 16 studies retrieved, seven were included in a meta-analysis, encompassing 1121 biological samples from various fish species. The detection limits reported ranged markedly from 0.07 to 34 copies/μL. A direct comparison of the diagnostic performance between ddPCR with quantitative PCR (qPCR) proved challenging due to limited data, thus only a pooled sensitivity analysis was feasible. The results showed a pooled sensitivity of 0.750 (95% confidence interval [CI]: 0.487–0.944) for ddPCR, compared to 0.461 (95% CI: 0.294–0.632) for qPCR, with no statistically significant difference in sensitivity between the two methods (<i>p</i> = .5884). Notably, significant heterogeneity was observed among the studies (<i>I</i><sup>2</sup> = 93%–97%, <i>p</i> &lt; .01), with the year of publication significantly influencing this heterogeneity (<i>p</i> &lt; .001), but not the country of origin (<i>p</i> = .49). No publication bias was detected, and the studies generally exhibited a low risk of bias according to QUADAS-C criteria. While ddPCR and qPCR showed comparable sensitivities in pathogen detection, ddPCR's capability to precisely quantify pathogens without the need for standard curves highlights its potential utility. This characteristic could significantly enhance the accuracy and reliability of pathogen detection in aquaculture.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 12","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Insights on the virulence and genomic features of Lactococcus garvieae isolated from giant freshwater prawn Macrobrachium rosenbergii (de Man 1879) 从巨型淡水对虾(Macrobrachium rosenbergii (de Man 1879) )中分离出的加维氏乳球菌(Lactococcus garvieae)的毒力和基因组特征的启示
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-09 DOI: 10.1111/jfd.14011
Rubicely Balan, Sudarshan Pandey, Pei-Chi Wang, Omkar Vijay Byadgi, Shih-Chu Chen

Giant freshwater prawn (Macrobrachium rosenbergii (MR)) is a significant aquafarm species commercially cultured in Taiwan. Intensive farming practices have led to the outbreak of Lactococcus garvieae (LG), which causes Lactococcosis in MR. Recently, LG has re-emerged and the number of mortalities in prawn farms has increased in Taiwan. However, there is no preventative strategy described and a lack of knowledge on virulence factors and pathogenesis from LG in MR. The most virulent strain of L. garvieae from M. rosenbergii was screened in vivo among seven isolates selected for infectivity testing injecting 0.1 mL of 108 CFU/mL bacterial concentration. Among the seven isolates screened, L. garvieae 109-6 resulted in 100% mortality within 3 days post-infection. Furthermore, 109-6 L. garvieae LD50 dosage from in MR was found to be 106 CFU/mL. Subsequently, the most virulent strain 109-6 was sequenced using MinIon Nanopore sequencing. Results indicated that the LG genome yielded a protein-coding of 3857 with 59 tRNA and 16 rRNA and no plasmid. Interestingly, the distribution of subsystems in the annotated genome revealed genes related to virulence, defence, and disease among LG 50 genes. Altogether, the virulent strain and its genome data revealed distinctive features of LG, which hinted toward its pathogenicity and could facilitate for better preventive strategies.

巨型淡水对虾(Macrobrachium rosenbergii,MR)是台湾商业养殖的重要水产养殖品种。集约化的养殖方式曾导致导致巨淡水对虾乳球菌病的 Lactococcus garvieae(LG)爆发。最近,LG 在台湾再次出现,对虾养殖场的死亡人数也有所增加。然而,目前尚无预防策略,也缺乏有关 MR 中 LG 的致病因子和致病机理的知识。通过注射0.1毫升108 CFU/毫升的细菌浓度进行感染性测试,从M. rosenbergii中筛选出7个分离株中毒性最强的L. garvieae。在筛选出的 7 个分离株中,L. garvieae 109-6 在感染后 3 天内导致 100%死亡。此外,在 MR 中发现 109-6 L. garvieae 的半数致死剂量为 106 CFU/mL。随后,使用 MinIon Nanopore 测序技术对毒性最强的菌株 109-6 进行了测序。结果表明,LG基因组产生了3857个蛋白质编码,其中有59个tRNA和16个rRNA,没有质粒。有趣的是,在 LG 50 个基因中,注释基因组的子系统分布显示了与毒力、防御和疾病有关的基因。总之,毒株及其基因组数据揭示了 LG 的显著特征,暗示了其致病性,有助于制定更好的预防策略。
{"title":"Insights on the virulence and genomic features of Lactococcus garvieae isolated from giant freshwater prawn Macrobrachium rosenbergii (de Man 1879)","authors":"Rubicely Balan,&nbsp;Sudarshan Pandey,&nbsp;Pei-Chi Wang,&nbsp;Omkar Vijay Byadgi,&nbsp;Shih-Chu Chen","doi":"10.1111/jfd.14011","DOIUrl":"10.1111/jfd.14011","url":null,"abstract":"<p>Giant freshwater prawn (<i>Macrobrachium rosenbergii</i> (MR)) is a significant aquafarm species commercially cultured in Taiwan. Intensive farming practices have led to the outbreak of <i>Lactococcus garvieae</i> (LG), which causes Lactococcosis in MR. Recently, LG has re-emerged and the number of mortalities in prawn farms has increased in Taiwan. However, there is no preventative strategy described and a lack of knowledge on virulence factors and pathogenesis from LG in MR. The most virulent strain of <i>L. garvieae</i> from <i>M. rosenbergii</i> was screened in vivo among seven isolates selected for infectivity testing injecting 0.1 mL of 10<sup>8</sup> CFU/mL bacterial concentration. Among the seven isolates screened, <i>L. garvieae</i> 109-6 resulted in 100% mortality within 3 days post-infection. Furthermore, 109-6 <i>L. garvieae</i> LD<sub>50</sub> dosage from in MR was found to be 10<sup>6</sup> CFU/mL. Subsequently, the most virulent strain 109-6 was sequenced using MinIon Nanopore sequencing. Results indicated that the LG genome yielded a protein-coding of 3857 with 59 tRNA and 16 rRNA and no plasmid. Interestingly, the distribution of subsystems in the annotated genome revealed genes related to virulence, defence, and disease among LG 50 genes. Altogether, the virulent strain and its genome data revealed distinctive features of LG, which hinted toward its pathogenicity and could facilitate for better preventive strategies.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 11","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142195983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ectopic thyroid adenoma diagnosed in a Senegal seabream (Diplodus bellottii, Steindachner 1882): Towards an accurate diagnosis 诊断出塞内加尔鲷(Diplodus bellottii, Steindachner 1882)患有异位甲状腺腺瘤:实现准确诊断。
IF 2.2 3区 农林科学 Q2 FISHERIES Pub Date : 2024-09-09 DOI: 10.1111/jfd.14015
Gabriela Fernandes da Silva, Fátima Faria, Fátima Gil, Nuno Leitão, Paulo Ribeiro, Katia Pinello, João Niza-Ribeiro, Irina Amorim

Thyroid tissue in teleosts is located mainly in the pharyngeal region, usually reaching other adjacent anatomical locations. Herein, a nodular lesion located in the left operculum of a Senegal seabream (Diplodus bellottii) was surgically excised and sent for microscopical evaluation. Microscopically, the lesion presented irregular borders and consisted in columnar epithelial cells arranged in a tubulopapillary pattern, surrounding a central lumen filled with acellular, acidophilic and homogeneous, material (‘colloid’). To determine the lesion's histogenesis, immunohistochemistry was performed employing antibodies for AE1/AE3, CK7, thyroglobulin and vimentin. The neoplastic cells presented low mitotic index and positive immunolabelling for CK7 and thyroglobulin. Therefore, a diagnosis of ectopic thyroid adenoma was made. Herein, the successful employment of antibodies classically used in mammals for accurate diagnosis of thyroid disorders is described. Proliferation of thyroid tissue in fish may reflect environmental and physiological imbalances, making the study and correct diagnosis of these tumours in this species important.

远洋鱼类的甲状腺组织主要位于咽部,通常会到达其他相邻的解剖位置。在此,我们对塞内加尔鲷(Diplodus bellottii)左侧厣的结节性病变进行了手术切除,并送去进行显微镜评估。显微镜下,病变呈不规则边界,由柱状上皮细胞组成,排列成管状乳头状,围绕着充满无细胞、嗜酸和均质物质("胶体")的中心腔。为了确定病变的组织发生机制,采用了 AE1/AE3、CK7、甲状腺球蛋白和波形蛋白抗体进行免疫组化。肿瘤细胞的有丝分裂指数较低,CK7和甲状腺球蛋白的免疫标记呈阳性。因此,诊断结果为异位甲状腺腺瘤。本文介绍了成功使用哺乳动物常用抗体准确诊断甲状腺疾病的方法。鱼类甲状腺组织的增生可能反映了环境和生理失衡,因此研究和正确诊断该物种的这些肿瘤非常重要。
{"title":"Ectopic thyroid adenoma diagnosed in a Senegal seabream (Diplodus bellottii, Steindachner 1882): Towards an accurate diagnosis","authors":"Gabriela Fernandes da Silva,&nbsp;Fátima Faria,&nbsp;Fátima Gil,&nbsp;Nuno Leitão,&nbsp;Paulo Ribeiro,&nbsp;Katia Pinello,&nbsp;João Niza-Ribeiro,&nbsp;Irina Amorim","doi":"10.1111/jfd.14015","DOIUrl":"10.1111/jfd.14015","url":null,"abstract":"<p>Thyroid tissue in teleosts is located mainly in the pharyngeal region, usually reaching other adjacent anatomical locations. Herein, a nodular lesion located in the left operculum of a Senegal seabream (<i>Diplodus bellottii</i>) was surgically excised and sent for microscopical evaluation. Microscopically, the lesion presented irregular borders and consisted in columnar epithelial cells arranged in a tubulopapillary pattern, surrounding a central lumen filled with acellular, acidophilic and homogeneous, material (‘colloid’). To determine the lesion's histogenesis, immunohistochemistry was performed employing antibodies for AE1/AE3, CK7, thyroglobulin and vimentin. The neoplastic cells presented low mitotic index and positive immunolabelling for CK7 and thyroglobulin. Therefore, a diagnosis of ectopic thyroid adenoma was made. Herein, the successful employment of antibodies classically used in mammals for accurate diagnosis of thyroid disorders is described. Proliferation of thyroid tissue in fish may reflect environmental and physiological imbalances, making the study and correct diagnosis of these tumours in this species important.</p>","PeriodicalId":15849,"journal":{"name":"Journal of fish diseases","volume":"47 12","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of fish diseases
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1