We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calcification and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was suppressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron microscopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could suggest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation.
{"title":"Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice","authors":"Kyoko Osawa, Takenori Kanai, Natsumi Ushijima, Koichiro Kajiwara, Yoshihiko Sawa, Yoshiaki Sato","doi":"10.2485/jhtb.32.213","DOIUrl":"https://doi.org/10.2485/jhtb.32.213","url":null,"abstract":"We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calcification and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was suppressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron microscopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could suggest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135260751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Yamada, H. Osanai, K. Shimada, Satoshi Murakami, Kozue Mori, H. Kuroiwa, N. Sugino, A. Taguchi
{"title":"Clinical Manifestations and The Size of Each Dentigerous Cyst by Computed Tomographic Findings","authors":"S. Yamada, H. Osanai, K. Shimada, Satoshi Murakami, Kozue Mori, H. Kuroiwa, N. Sugino, A. Taguchi","doi":"10.2485/jhtb.32.139","DOIUrl":"https://doi.org/10.2485/jhtb.32.139","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"38 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mizuki Yoshida, Saki Takahashi, Nanako Tsuchimochi, H. Ishii, T. Naito, J. Ohno
{"title":"Etoposide-Induced Cellular Senescence Suppresses Autophagy in Human Keratinocytes","authors":"Mizuki Yoshida, Saki Takahashi, Nanako Tsuchimochi, H. Ishii, T. Naito, J. Ohno","doi":"10.2485/jhtb.32.183","DOIUrl":"https://doi.org/10.2485/jhtb.32.183","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"47 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68967096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuhei Nomura, S. Matsunaga, M. Yamada, N. Kasahara, S. Abe, M. Furusawa
{"title":"Age-Related Differences in Side Walls of Maxillary First Molar Pulp Cavities in Japanese Using Micro-Computed Tomography","authors":"Shuhei Nomura, S. Matsunaga, M. Yamada, N. Kasahara, S. Abe, M. Furusawa","doi":"10.2485/jhtb.32.191","DOIUrl":"https://doi.org/10.2485/jhtb.32.191","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68967146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Study on the Effect of Soft-Start Light on Microleakage in Pit and Fissure Closure","authors":"Yong Chen, Ling Guo","doi":"10.2485/jhtb.32.105","DOIUrl":"https://doi.org/10.2485/jhtb.32.105","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Low Concentration of Pingyangmycin Combined with Dexamethasone Injection in the Treatment of Superficial Lip Venous Malformations","authors":"Yanhong Ma, Ruihan Wu, Hao Zheng, Jian Meng, Kun Han, Xinbao Gao, Jingjian Wei","doi":"10.2485/jhtb.32.151","DOIUrl":"https://doi.org/10.2485/jhtb.32.151","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Study of Submandibular Gland Changes in Mice of a Murine Model of Sjögren’s Syndrome Administered Dental Pulp Stem Cell-Conditioned Medium","authors":"Seiko Usui, Haruka Takahashi, A. Tanaka","doi":"10.2485/jhtb.32.119","DOIUrl":"https://doi.org/10.2485/jhtb.32.119","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuki Hayashi, Shintaro Sakatoku, Y. Sugita, Taku Futenma, N. Iida, Keisuke Nakamura, H. Nawa
{"title":"A Potential Biomarker of Dental Pulp Regeneration: Wnt10a","authors":"Yuki Hayashi, Shintaro Sakatoku, Y. Sugita, Taku Futenma, N. Iida, Keisuke Nakamura, H. Nawa","doi":"10.2485/jhtb.32.197","DOIUrl":"https://doi.org/10.2485/jhtb.32.197","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68967154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nobutake Monai, Atsushi Kuwabara, Norishige Kawanishi, R. Ozawa, Takuya Adachi, Saki Tsunoi, Makoto Inoue, M. Saita, T. Hayakawa, Noriyuki Hoshi, K. Kimoto
{"title":"Effect of UV Photofunctionalization of HA/TiO2 Coated Implants Prepared by Dual-Target Sputtering on Bone-Implant Integration","authors":"Nobutake Monai, Atsushi Kuwabara, Norishige Kawanishi, R. Ozawa, Takuya Adachi, Saki Tsunoi, Makoto Inoue, M. Saita, T. Hayakawa, Noriyuki Hoshi, K. Kimoto","doi":"10.2485/jhtb.32.99","DOIUrl":"https://doi.org/10.2485/jhtb.32.99","url":null,"abstract":"","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":"146 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68966891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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