Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.8
M. H. Khani, A. Zireh, E. A. Adegani
Hankel matrices are related to a wide range of disparate determinant computations and algorithms and some very attractive computational properties are allocated to them. Also, the Hankel determinants are crucial factors in the research of singularities and power series with integral coefficients. It is specified that the Fekete-Szego functional and the second Hankel determinant are equivalent to H 1 (2) and H 2 (2), respectively. In this study, the upper bounds were obtained for the second Hankel determinant of the subclass of bi-univalent functions, which is defined by subordination. It is worth noticing that the bounds rendered in the present paper generalize and modify some previous results.
{"title":"The Second Hankel Determinant Problem for a Class of Bi-Univalent Functions","authors":"M. H. Khani, A. Zireh, E. A. Adegani","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.8","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.8","url":null,"abstract":"Hankel matrices are related to a wide range of disparate determinant computations and algorithms and some very attractive computational properties are allocated to them. Also, the Hankel determinants are crucial factors in the research of singularities and power series with integral coefficients. It is specified that the Fekete-Szego functional and the second Hankel determinant are equivalent to H 1 (2) and H 2 (2), respectively. In this study, the upper bounds were obtained for the second Hankel determinant of the subclass of bi-univalent functions, which is defined by subordination. It is worth noticing that the bounds rendered in the present paper generalize and modify some previous results.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"17 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78022734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.3
A. Safitri, A. Srihardyastutie, A. Roosdiana, A. Aulanni’am, Eva Nur Laili Octaviana
The aim of this research was to study the antidiabetic activity of root extract of Ruellia tuberosa L. on rats ( Rattus novergicus ) induced by multiple low doses of streptozotocin. The parameters investigated were blood glucose levels, protease activities, and the histopathologic profiles of the kidneys. Three groups of rats, i.e. control, diabetic, and treatment, were used. R. tuberosa L. extracts were given orally for treatment at a dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extract of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes only. Moreover, antidiabetic activity of R. tuberosa L extract was evidenced by the 55% lower protease activity in the treatment group compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in the histopathologic profiles of the kidneys in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.
{"title":"Effects of Root Extract of Ruellia tuberosa L. on Kidneys of Diabetic Rats","authors":"A. Safitri, A. Srihardyastutie, A. Roosdiana, A. Aulanni’am, Eva Nur Laili Octaviana","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.3","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.3","url":null,"abstract":"The aim of this research was to study the antidiabetic activity of root extract of Ruellia tuberosa L. on rats ( Rattus novergicus ) induced by multiple low doses of streptozotocin. The parameters investigated were blood glucose levels, protease activities, and the histopathologic profiles of the kidneys. Three groups of rats, i.e. control, diabetic, and treatment, were used. R. tuberosa L. extracts were given orally for treatment at a dose of 250 mg/kg body weight per day for 21 consecutive days. The induced diabetes group treated with root extract of R. tuberosa L. (group III) had 60.3% lower glucose levels (119 mg/dL) compared to the group with induced diabetes only. Moreover, antidiabetic activity of R. tuberosa L extract was evidenced by the 55% lower protease activity in the treatment group compared to the untreated diabetic group. Finally, a positive effect from root extract of R. tuberosa L. was shown from the less damaged renal glomerulus and smaller gap between extracellular matrices and epithelium cells in the histopathologic profiles of the kidneys in the extract treatment group. These results show the potential of root extract of R. tuberosa L. as a natural product anti-diabetic agent.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"90 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76316819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.2
C. Retnaningdyah, E. Arisoesilaningsih
The aim of this study was to evaluate the effectivity of batch culture phytoremediation to remediate irrigation water that was contaminated with 6.4 µL/L synthetic pesticide Prevathon. This was a true experimental research study using a completely randomized design, conducted in a 30 L bucket with sand and gravel as the substrate. The treatment comprised five types of hydromacrophytes (emergent, floating leaf, submerged, polyculture and control without plants), repeated three times at the same time. The effectiveness of the phytoremediation model was determined by water physicochemical parameters, periphyton diversity, percentage of pollution tolerant values (%PTV) and trophic diatom index (TDI) on day 6, 13, 29 and 37 after incubation. The research results showed significantly decreased values of biochemical oxygen demand, turbidity, bicarbonate, nitrate, orthophosphate, total phosphate and free chlorine after incubation for 6 days; decreased concentration of chemical oxygen demand after incubation for 13 days. The water quality improved from slightly and moderately polluted (diversity index 1.6-2.8) to clean and slightly polluted (diversity index 2.8-4.2), from moderately and heavily organically polluted (PTV 40.0-93.7%) to slightly organically polluted and free of organic pollution (PTV 2.4-34.1%), and from meso-eutrophic and eutrophic (TDI 37.4-70.4) to oligo and meso-eutrophic (TDI 13.7-26.4).
{"title":"Evaluation of Batch Culture Phytoremediation Process using Local Hydromacrophytes to Reduce Synthetic Pesticide Residue in Contaminated Irrigation Water","authors":"C. Retnaningdyah, E. Arisoesilaningsih","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.2","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.2","url":null,"abstract":"The aim of this study was to evaluate the effectivity of batch culture phytoremediation to remediate irrigation water that was contaminated with 6.4 µL/L synthetic pesticide Prevathon. This was a true experimental research study using a completely randomized design, conducted in a 30 L bucket with sand and gravel as the substrate. The treatment comprised five types of hydromacrophytes (emergent, floating leaf, submerged, polyculture and control without plants), repeated three times at the same time. The effectiveness of the phytoremediation model was determined by water physicochemical parameters, periphyton diversity, percentage of pollution tolerant values (%PTV) and trophic diatom index (TDI) on day 6, 13, 29 and 37 after incubation. The research results showed significantly decreased values of biochemical oxygen demand, turbidity, bicarbonate, nitrate, orthophosphate, total phosphate and free chlorine after incubation for 6 days; decreased concentration of chemical oxygen demand after incubation for 13 days. The water quality improved from slightly and moderately polluted (diversity index 1.6-2.8) to clean and slightly polluted (diversity index 2.8-4.2), from moderately and heavily organically polluted (PTV 40.0-93.7%) to slightly organically polluted and free of organic pollution (PTV 2.4-34.1%), and from meso-eutrophic and eutrophic (TDI 37.4-70.4) to oligo and meso-eutrophic (TDI 13.7-26.4).","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83638352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.5
A. O. Atagün, Hüseyin Kamacı, İsmail Taştekin, A. Sezgi̇n
In this paper, assuming that N is a near-ring and P is an ideal of N , the P -center of N , the P -center of an element in N, the P -identities of N are defined. Their properties and relations are investigated. It is shown that the set of all P -identities in N is a multiplicative subsemigroup of N . Also, P -right and P -left permutable and P -medial near-rings are defined and some properties and connections are given. P -regular and P -strongly regular near-rings are studied. P -completely prime ideals are introduced and some characterizations of -completely prime near-rings are provided. Also, some properties of P -idempotents, P -centers, P -identities in P -completely prime near-rings are investigated. The results that were obtained in this study are illustrated with many examples.
{"title":"P-properties in Near-rings","authors":"A. O. Atagün, Hüseyin Kamacı, İsmail Taştekin, A. Sezgi̇n","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.5","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.5","url":null,"abstract":"In this paper, assuming that N is a near-ring and P is an ideal of N , the P -center of N , the P -center of an element in N, the P -identities of N are defined. Their properties and relations are investigated. It is shown that the set of all P -identities in N is a multiplicative subsemigroup of N . Also, P -right and P -left permutable and P -medial near-rings are defined and some properties and connections are given. P -regular and P -strongly regular near-rings are studied. P -completely prime ideals are introduced and some characterizations of -completely prime near-rings are provided. Also, some properties of P -idempotents, P -centers, P -identities in P -completely prime near-rings are investigated. The results that were obtained in this study are illustrated with many examples.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"65 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73391896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.6
E. Julianti, S. Susanti, M. Singgih, Laida Neti Mulyani
Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.
螺旋藻是一种含有藻蓝蛋白的微藻，藻蓝蛋白是一种蓝色色素，广泛用于食品、化妆品和制药行业。本研究的目的是从干燥的螺旋藻生物量中分离藻蓝蛋白。以0.01 M pH为7的磷酸盐缓冲液进行冻融提取，提取效果最佳。采用50%硫酸铵沉淀、透析和凝胶过滤层析进行纯化。纯化产物的纯度比为1.729 (A615/A280)。利用SDS-PAGE对分离物进行了表征，得到了15-16 Kda左右的分子量，并利用红外分光光度法获得了与标准藻蓝蛋白相似的光谱。两种方法均证实该分离物为藻蓝蛋白。
{"title":"Optimization of Extraction Method and Characterization of Phycocyanin Pigment from Spirulina platensis","authors":"E. Julianti, S. Susanti, M. Singgih, Laida Neti Mulyani","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.6","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.6","url":null,"abstract":"Spirulina platensis is a microalgae species that contains phycocyanin, a blue pigment that is widely used in the food, cosmetic and pharmaceutical industries. The objective of this study was to isolate phycocyanin from dried biomass of Spirulina platensis. The optimum extraction was through freeze-thawing extraction with 0.01 M phosphate buffer pH 7. Purification was done using 50% ammonium sulphate precipitation, dialysis, and gel filtration chromatography. The purity ratio of the purified product was 1.729 (A615/A280). Characterization of isolate, carried out using SDS-PAGE, gave molecular weights of around 15-16 Kda and using infrared spectrophotometry the similarity with the standard phycocyanin spectrum was obtained. Both methods confirmed that the isolate was phycocyanin.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72522793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-06DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.7
W. Srigutomo, M. Heriyanto, M. H. Aufa
Global approaches for estimating geophysical model parameters have been proposed by several authors, including their application for gravity interpretation, which is currently limited to simple and fixed geometrical problems. This paper proposes implementation of Very Fast Simulated Annealing (VFSA) in two-dimensional gravity interpretation problems, which are still rarely addressed. The modeling domain was divided into smaller sub-domains and gravity anomaly calculation was carried out based on the Talwani formulation. To improve the uniqueness of the solution of under-determined problems, specific constraints were added in addition to the assumed known symmetry axes. The inversion of VFSA was tested on synthetic data generated by simple models and on previously published real data to evaluate the applicability of the proposed approach to the interpretation of field data.
{"title":"Gravity Inversion of Talwani Model using Very Fast Simulated Annealing","authors":"W. Srigutomo, M. Heriyanto, M. H. Aufa","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.7","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.7","url":null,"abstract":"Global approaches for estimating geophysical model parameters have been proposed by several authors, including their application for gravity interpretation, which is currently limited to simple and fixed geometrical problems. This paper proposes implementation of Very Fast Simulated Annealing (VFSA) in two-dimensional gravity interpretation problems, which are still rarely addressed. The modeling domain was divided into smaller sub-domains and gravity anomaly calculation was carried out based on the Talwani formulation. To improve the uniqueness of the solution of under-determined problems, specific constraints were added in addition to the assumed known symmetry axes. The inversion of VFSA was tested on synthetic data generated by simple models and on previously published real data to evaluate the applicability of the proposed approach to the interpretation of field data.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89754212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.4
Sajjad Ahmad, Maimoona Gull, Asma Shah, F. Khan, M. Rafiq, Ghusia Lutfullah, Alam Khan, Farhat Amin, N. Azhar, M. S. Khan
In this study, different aerial parts of Monotheca Buxifolia were screened for their antibacterial, antifungal, and anti-oxidant activity . Extract of the fruit was most active against P. mirabilis, MSSA and M. morganii strains. Moreover, the methanolic fruit extract demonstrated good antibacterial properties against VRSA, S. aureus and Acinetobacter . Despite having good antibacterial properties, Monotheca buxifolia showed low antifungal activity. The cytotoxic activity was the highest in the pet-ether fruit extract, causing 60% of lethality against Artemia salina larvae with LD 50 at 3.307. The highest scavenging activity was shown by the methanolic leaf extract in a concentration-dependent pattern. The findings of this study suggest that Monotheca Buxifolia has good antibacterial, antioxidant potential but low antifungal properties.
本研究对Buxifolia不同地上部位的抗菌、抗真菌和抗氧化活性进行了筛选。果实提取物对mirabilis P. mirabilis、MSSA和morganii最有效。此外，甲醇果提取物对VRSA、金黄色葡萄球菌和不动杆菌具有良好的抗菌性能。尽管有良好的抗菌性能，但苦叶单叶的抗真菌活性较低。对盐渍蒿幼虫的细胞毒活性最高，致毒率为60%，ld50为3.307。甲醇叶提取物的清除率最高，且呈浓度依赖性。本研究结果表明，Buxifolia具有良好的抗菌、抗氧化潜力，但抗真菌性能较差。
{"title":"Antimicrobial, Antioxidant and Cytotoxic Potential of Aerial Parts of Monotheca buxifolia","authors":"Sajjad Ahmad, Maimoona Gull, Asma Shah, F. Khan, M. Rafiq, Ghusia Lutfullah, Alam Khan, Farhat Amin, N. Azhar, M. S. Khan","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.4","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.4","url":null,"abstract":"In this study, different aerial parts of Monotheca Buxifolia were screened for their antibacterial, antifungal, and anti-oxidant activity . Extract of the fruit was most active against P. mirabilis, MSSA and M. morganii strains. Moreover, the methanolic fruit extract demonstrated good antibacterial properties against VRSA, S. aureus and Acinetobacter . Despite having good antibacterial properties, Monotheca buxifolia showed low antifungal activity. The cytotoxic activity was the highest in the pet-ether fruit extract, causing 60% of lethality against Artemia salina larvae with LD 50 at 3.307. The highest scavenging activity was shown by the methanolic leaf extract in a concentration-dependent pattern. The findings of this study suggest that Monotheca Buxifolia has good antibacterial, antioxidant potential but low antifungal properties.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"52 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90991496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.5614/J.MATH.FUND.SCI.2019.51.2.1
Ervi Afifah, T. Mozef, F. Sandra, Seila Arumwardana, Dwi Davidson Rihibiha, H. Nufus, R. Rizal, A. Amalia, I. Bachtiar, Harry Murti, W. Widowati
Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON-002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP-2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON-002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.
{"title":"Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model","authors":"Ervi Afifah, T. Mozef, F. Sandra, Seila Arumwardana, Dwi Davidson Rihibiha, H. Nufus, R. Rizal, A. Amalia, I. Bachtiar, Harry Murti, W. Widowati","doi":"10.5614/J.MATH.FUND.SCI.2019.51.2.1","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.2.1","url":null,"abstract":"Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON-002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP-2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON-002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84813726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-30DOI: 10.5614/J.MATH.FUND.SCI.2019.51.1.6
I. A. Dewanti, I. Susilawati, P. Lestari, Ristya Widi Endah Lestari, Erawati Wulandari, Roedy Budirahardjo, Dyah Setyorini, Sunlip Wibisono
Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14‑, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.
{"title":"Robusta Coffee (Coffeacanephora) Decreasing IL-1α (Interleukin-1α) Expression and Increasing the Number of Fibroblasts in Healing Process in Dental Pulp in Wistar Rats","authors":"I. A. Dewanti, I. Susilawati, P. Lestari, Ristya Widi Endah Lestari, Erawati Wulandari, Roedy Budirahardjo, Dyah Setyorini, Sunlip Wibisono","doi":"10.5614/J.MATH.FUND.SCI.2019.51.1.6","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.1.6","url":null,"abstract":"Fibroblasts and IL-1α are important components of inflammation and healing in dental caries. This healing allegedly could be enhanced by robusta coffee. The aim of this study was to investigate the effect of varying concentrations of robusta coffee on IL-1α expression and the number of fibroblasts. Wistar rats were used, divided into 4 groups and 3 subgroups (7-, 14‑, and 21-day rats) with each group containing 4 animals: Control group: untreated group; 25% Coffee Group: cavities + treated with 25% coffee bean paste; 50% Coffee Group: cavities + treated with 50% coffee bean paste; 75% Coffee Group: cavities + treated with 75% coffee bean paste. The 7-, 14-, and 21-day rats were sacrificed serially for analysis of the number of dental pulp fibroblasts by staining with HE and IL-1α expression by immunohistochemistry. The resulting data were analyzed descriptively and using ANOVA, followed by an LSD test. The higher the concentration of robusta coffee, the more the expression of IL-1α decreased and the more the number of fibroblasts increased. It is suspected that immunomodulatory proteins inhibit IL-1α expression. It was concluded that robusta coffee could decrease IL-1α expression and increase the number of fibroblasts during the healing process in the dental pulp of Wistar rats.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"30 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78300115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-30DOI: 10.5614/J.MATH.FUND.SCI.2019.51.1.7
Rudy Prihantoro, D. Sutarno, N. Nurhasan
As exploration is forced into more difficult areas with complex three-dimensional (3-D) structural environments, the importance of 3-D magnetotelluric (MT) modeling is essential for the correct interpretation of MT data. To reduce the complexity of the calculations introduced by 3-D models, iterative forward calculation of MT response functions is used as basis for inversion of 3-D MT data. This paper proposes an alternative procedure for making reliable 3-D MT modeling codes for forward calculation that is not only effective but also accurate. This is accomplished by using a direct method to solve the linear systems arising from the discretization process in the vector finite element approach. The vector finite element method is known for its capability of overcoming difficulties in modeling caused by possible jumps of normal components across discontinuities of material properties. Meanwhile, by using a direct method rather than an iterative method, the process of solving the linear equations does not suffer from slow convergence. Here, we present a comparison between our modeling codes and codes based on a different approach. In the resulting 3-D MT responses it was found that the proposed method has high accuracy.
{"title":"Direct Method Solution of 3-D Magnetotelluric Modeling Using Vector Finite Element Method","authors":"Rudy Prihantoro, D. Sutarno, N. Nurhasan","doi":"10.5614/J.MATH.FUND.SCI.2019.51.1.7","DOIUrl":"https://doi.org/10.5614/J.MATH.FUND.SCI.2019.51.1.7","url":null,"abstract":"As exploration is forced into more difficult areas with complex three-dimensional (3-D) structural environments, the importance of 3-D magnetotelluric (MT) modeling is essential for the correct interpretation of MT data. To reduce the complexity of the calculations introduced by 3-D models, iterative forward calculation of MT response functions is used as basis for inversion of 3-D MT data. This paper proposes an alternative procedure for making reliable 3-D MT modeling codes for forward calculation that is not only effective but also accurate. This is accomplished by using a direct method to solve the linear systems arising from the discretization process in the vector finite element approach. The vector finite element method is known for its capability of overcoming difficulties in modeling caused by possible jumps of normal components across discontinuities of material properties. Meanwhile, by using a direct method rather than an iterative method, the process of solving the linear equations does not suffer from slow convergence. Here, we present a comparison between our modeling codes and codes based on a different approach. In the resulting 3-D MT responses it was found that the proposed method has high accuracy.","PeriodicalId":16255,"journal":{"name":"Journal of Mathematical and Fundamental Sciences","volume":"123 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75945441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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