Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.11.956
D. Im, K. Lee
{"title":"LC-MS/MS Screening Method for Radical Scavenging Active Compounds in Extracts of Ulmus pumila Cortex","authors":"D. Im, K. Lee","doi":"10.5352/JLS.2020.30.11.956","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.11.956","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87371035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.4.359
Min-Kyung Park, Se-hee Cho, Tae-kyu Ahn, Do Hyun Kim, Soyeon Kim, Jinwook Lee, Jee-in Kim, E. Seo, K. Son, Jae-hwan Lim
{"title":"Immunomodulatory Effects of β-sitosterol and Daucosterol Isolated from Dioscorea batatas on LPS-stimulated RAW 264.7 and TK-1 Cells","authors":"Min-Kyung Park, Se-hee Cho, Tae-kyu Ahn, Do Hyun Kim, Soyeon Kim, Jinwook Lee, Jee-in Kim, E. Seo, K. Son, Jae-hwan Lim","doi":"10.5352/JLS.2020.30.4.359","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.4.359","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85734142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.10.886
Yong pyo Shin, Joon-Ha Lee, In-woo Kim, M. Seo, Mi-Ae Kim, H. Lee, Minhee Baek, Seong Hyun Kim, Jae‐Sam Hwang
The development of novel peptide antibiotics with potent antimicrobial activity and anti-inflammatory activity is urgently needed. In a previous work, we performed an in-silico analysis of the Papilio xuthus transcriptome to identify putative antimicrobial peptides and identified several candidates. In this study, we investigated the antibacterial and anti-inflammatory activities of papiliocin 3, which was selected bioinformatically based on its physicochemical properties against bacteria and mouse macrophage Raw264.7 cells. Papiliocin 3 showed antibacterial activities against E. coli and S. aureus without inducing hemolysis and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Moreover, ELISA and Western blot analysis revealed that papiliocin 3 reduced the expression levels of pro-inflammatory enzymes, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). In addition, we examined whether papiliocin 3 could inhibit the expression of pro-inflammatory cytokines (interleukin-6 and interleukin-1β) in LPSinduced Raw264.7 cells. We found that papiliocin 3 markedly reduced the expression level of cytokines through the regulation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa B (NF-κB) signaling. We also confirmed that papiliocin 3 binds to bacterial cell membranes via a specific interaction with lipopolysaccharides. Collectively, these findings suggest that papiliocin 3 could be a promising molecule for development as a novel peptide antibiotic.
{"title":"Anti-inflammatory Activity of Antimicrobial Peptide Papiliocin 3 Derived from the Swallowtail Butterfly, Papilio xuthus","authors":"Yong pyo Shin, Joon-Ha Lee, In-woo Kim, M. Seo, Mi-Ae Kim, H. Lee, Minhee Baek, Seong Hyun Kim, Jae‐Sam Hwang","doi":"10.5352/JLS.2020.30.10.886","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.10.886","url":null,"abstract":"The development of novel peptide antibiotics with potent antimicrobial activity and anti-inflammatory activity is urgently needed. In a previous work, we performed an in-silico analysis of the Papilio xuthus transcriptome to identify putative antimicrobial peptides and identified several candidates. In this study, we investigated the antibacterial and anti-inflammatory activities of papiliocin 3, which was selected bioinformatically based on its physicochemical properties against bacteria and mouse macrophage Raw264.7 cells. Papiliocin 3 showed antibacterial activities against E. coli and S. aureus without inducing hemolysis and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Moreover, ELISA and Western blot analysis revealed that papiliocin 3 reduced the expression levels of pro-inflammatory enzymes, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2). In addition, we examined whether papiliocin 3 could inhibit the expression of pro-inflammatory cytokines (interleukin-6 and interleukin-1β) in LPSinduced Raw264.7 cells. We found that papiliocin 3 markedly reduced the expression level of cytokines through the regulation of mitogen-activated protein kinases (MAPK) and nuclear factor kappa B (NF-κB) signaling. We also confirmed that papiliocin 3 binds to bacterial cell membranes via a specific interaction with lipopolysaccharides. Collectively, these findings suggest that papiliocin 3 could be a promising molecule for development as a novel peptide antibiotic.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80533718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.3.298
Su-Jin Pyo, Yun-jin Lee, Deok-Gyeong Kang, Ho-Jun Son, G. Park, Jong-Yi Park, H. Sohn
{"title":"Antimicrobial, Antioxidant, and Anti-diabetic Activities of Rodgersia podophylla","authors":"Su-Jin Pyo, Yun-jin Lee, Deok-Gyeong Kang, Ho-Jun Son, G. Park, Jong-Yi Park, H. Sohn","doi":"10.5352/JLS.2020.30.3.298","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.3.298","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79018104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.10.877
J. Lee, J. Oh, Soyoung Park, Hye-Ran Kim, K. Jung, Byung-Jin Jeon, Dong-Min Kim, C. Kong
Lentinuls edodes Jung Im Lee, Jung Hwan Oh, So Young Park, Hye Ran Kim, Kyung Im Jung, Byung-Jin Jeon, Dongmin Kim and Chang-Suk Kong* Marine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Korea Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea 3 Busan Technopark, Busan 46048, Korea
{"title":"UV-induced Photodamage - attenuating Properties of Water Extract from Lentinuls edodes","authors":"J. Lee, J. Oh, Soyoung Park, Hye-Ran Kim, K. Jung, Byung-Jin Jeon, Dong-Min Kim, C. Kong","doi":"10.5352/JLS.2020.30.10.877","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.10.877","url":null,"abstract":"Lentinuls edodes Jung Im Lee, Jung Hwan Oh, So Young Park, Hye Ran Kim, Kyung Im Jung, Byung-Jin Jeon, Dongmin Kim and Chang-Suk Kong* Marine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University, Busan 46958, Korea Department of Food and Nutrition, College of Medical and Life Sciences, Silla University, Busan 46958, Korea 3 Busan Technopark, Busan 46048, Korea","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74317025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.1.77
BeomYong Kim, Hyung-Bin Ham, Sang-Hyeon Kim, J. Son, K. Chung
{"title":"Assessing the Use of 5 ml Straws in the Cryopreservation of Boar Semen","authors":"BeomYong Kim, Hyung-Bin Ham, Sang-Hyeon Kim, J. Son, K. Chung","doi":"10.5352/JLS.2020.30.1.77","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.1.77","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75634935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.11.1007
Kwan-Woo Kim, Jinwook Lee, Eun-Do Lee, Sung-Soo Lee, You-Lim Choi, Hyunsun Lim, Yousam Kim, Sang-Hoon Lee
Monomorphic SNP Data of the Korean Native Black Goat and Crossbred Goat Kwan-Woo Kim, Jinwook Lee, Eun-Do Lee, Sung-Soo Lee, You-Lim Choi, Hyun-Tae Lim, Yousam Kim and Sang-Hoon Lee* Animal Genetics Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea Animal Genetics & Breeding Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea 3 Department of animal science, Gyeongsang National University, Jinju 52828, Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea TNT Research Co., Ltd., Anyang-si, Gyeonggi-do 14059, Korea
{"title":"Comparison and Validation of Genetic Diversity and Population Structure Using Monomorphic SNP Data of the Korean Native Black Goat and Crossbred Goat","authors":"Kwan-Woo Kim, Jinwook Lee, Eun-Do Lee, Sung-Soo Lee, You-Lim Choi, Hyunsun Lim, Yousam Kim, Sang-Hoon Lee","doi":"10.5352/JLS.2020.30.11.1007","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.11.1007","url":null,"abstract":"Monomorphic SNP Data of the Korean Native Black Goat and Crossbred Goat Kwan-Woo Kim, Jinwook Lee, Eun-Do Lee, Sung-Soo Lee, You-Lim Choi, Hyun-Tae Lim, Yousam Kim and Sang-Hoon Lee* Animal Genetics Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea Animal Genetics & Breeding Division, National Institute of Animal Science, RDA, Cheonan 31000, Korea 3 Department of animal science, Gyeongsang National University, Jinju 52828, Korea Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea TNT Research Co., Ltd., Anyang-si, Gyeonggi-do 14059, Korea","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84583575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.12.1109
Sunyoung Kim, Kyu‐Won Kwak, K. Lee, Hyeon-Jin Ko, Yong‐Soon Kim, Eun Sun Kim, K. Park, H. Yoon
{"title":"Comparative Analysis of Nutritional Components of Zophobas atratus Larvae Raised with Artificial Diet and Wheat Bran","authors":"Sunyoung Kim, Kyu‐Won Kwak, K. Lee, Hyeon-Jin Ko, Yong‐Soon Kim, Eun Sun Kim, K. Park, H. Yoon","doi":"10.5352/JLS.2020.30.12.1109","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.12.1109","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78241934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.7.581
J. Ohn, Min-Kyeong Seol, E. Bae, Young-je Cho, H. Jung, Byung-Oh Kim
This study investigated the probiotic properties and physiological activities of Korean fermented foods such as sikhae, young radish kimchi, and bean-curd dregs. Among the isolated lactic acid bacteria, Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, and Leuconostoc mesenteroides YMT6 demonstrated a greater than 60% survival rate at pH 2.5, along with an excellent survival rate even at 0.3% bile acid. These five bacteria showed strong flocculation ability in autoaggregation and coaggregation tests, indirectly clustering useful micro-organisms and inhibiting the attachment of pathogenic bacteria. In a cell surface hydrophobicity test, these bacteria showed adhesion to three solvents (ethyl acetate, chloroform, and xylene) and high hydrophobicity, thereby indicating excellent indirect cell adhesion to intestinal cells. The cell-free supernatants and intracellular extracts of the five lactic acid bacteria showed antioxidative activity in the form of 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability and lipid peroxidation inhibition. Antimicrobial activities were also observed in four pathogenic bacteria, namely E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, and S. aureus KCTC 1916. These results demonstrate that these five lactic acid bacteria could be used as probiotics with antioxidant and antimicrobial properties.
{"title":"The Potential Probiotic and Functional Health Effects of Lactic Acid Bacteria Isolated from Traditional Korean Fermented Foods","authors":"J. Ohn, Min-Kyeong Seol, E. Bae, Young-je Cho, H. Jung, Byung-Oh Kim","doi":"10.5352/JLS.2020.30.7.581","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.7.581","url":null,"abstract":"This study investigated the probiotic properties and physiological activities of Korean fermented foods such as sikhae, young radish kimchi, and bean-curd dregs. Among the isolated lactic acid bacteria, Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, and Leuconostoc mesenteroides YMT6 demonstrated a greater than 60% survival rate at pH 2.5, along with an excellent survival rate even at 0.3% bile acid. These five bacteria showed strong flocculation ability in autoaggregation and coaggregation tests, indirectly clustering useful micro-organisms and inhibiting the attachment of pathogenic bacteria. In a cell surface hydrophobicity test, these bacteria showed adhesion to three solvents (ethyl acetate, chloroform, and xylene) and high hydrophobicity, thereby indicating excellent indirect cell adhesion to intestinal cells. The cell-free supernatants and intracellular extracts of the five lactic acid bacteria showed antioxidative activity in the form of 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability and lipid peroxidation inhibition. Antimicrobial activities were also observed in four pathogenic bacteria, namely E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, and S. aureus KCTC 1916. These results demonstrate that these five lactic acid bacteria could be used as probiotics with antioxidant and antimicrobial properties.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88465935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5352/JLS.2020.30.10.905
Ga-Young Lee, Min Jee Kim, So Yeon Kim, K. Lee, D. Oh, Y. Cho, Y. Yoo
The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 μ g/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 μg/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+ PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.
{"title":"Adjuvant Effect of PAMAM Dendrimer on the Antigenicity of Keyhole Limpet Hemocyanin in Balb/c Mice","authors":"Ga-Young Lee, Min Jee Kim, So Yeon Kim, K. Lee, D. Oh, Y. Cho, Y. Yoo","doi":"10.5352/JLS.2020.30.10.905","DOIUrl":"https://doi.org/10.5352/JLS.2020.30.10.905","url":null,"abstract":"The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 μ g/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 μg/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+ PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83723225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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