Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.144
H. Choi, H. Kim, S. Kim, S. Han
This study aimed to analyze the content and composition of a biological amine, cadaverine, isolated from the venom of worker honeybees (Apis mellifera L.). This biological amine—which has diverse functionality, such as anti-inflammatory and antibacterial effects—has not been previously reported in bee venom. An assay completed in 13 minutes was developed for the cadaverine present in the bee venom using an ultra-performance liquid chromatograph and a Halo C18 column with acetonitrile and water as the mobile phase. The specificity, accuracy, and precision of the assay were verified, and the assay was validated. The linearity for cadaverine in the bee venom was R=0.99 or above, indicating a moderate level. The limit of detection and limit of quantification were both 0.3 μg/ml, and the rate of recovery was 97.6%-99.1%. The relative standard deviation (RSD) of the intra-day precision and inter-day precision for cadaverine was 0.25%-0.44% and 0.25%-1.25%, respectively, with an RSD that fell within 5% indicating excellent precision. Through this novel assay, it was found that the mean content of cadaverine was 1.10±0.05 mg/g. Our results indicated that the linearity, limit of detection, limit of quantification, and rate of recovery of the cadaverine assay were of a satisfactory level, and the cadaverine content of the bee venom was ably determined. This study provides basic data on cadaverine in bee venom, which will prove useful in further studies on the bioactivity of this component.
{"title":"Analysis of Cadaverine and Its Worker Honeybee Venom Content (Apis mellifera L.)","authors":"H. Choi, H. Kim, S. Kim, S. Han","doi":"10.5352/JLS.2021.31.2.144","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.144","url":null,"abstract":"This study aimed to analyze the content and composition of a biological amine, cadaverine, isolated from the venom of worker honeybees (Apis mellifera L.). This biological amine—which has diverse functionality, such as anti-inflammatory and antibacterial effects—has not been previously reported in bee venom. An assay completed in 13 minutes was developed for the cadaverine present in the bee venom using an ultra-performance liquid chromatograph and a Halo C18 column with acetonitrile and water as the mobile phase. The specificity, accuracy, and precision of the assay were verified, and the assay was validated. The linearity for cadaverine in the bee venom was R=0.99 or above, indicating a moderate level. The limit of detection and limit of quantification were both 0.3 μg/ml, and the rate of recovery was 97.6%-99.1%. The relative standard deviation (RSD) of the intra-day precision and inter-day precision for cadaverine was 0.25%-0.44% and 0.25%-1.25%, respectively, with an RSD that fell within 5% indicating excellent precision. Through this novel assay, it was found that the mean content of cadaverine was 1.10±0.05 mg/g. Our results indicated that the linearity, limit of detection, limit of quantification, and rate of recovery of the cadaverine assay were of a satisfactory level, and the cadaverine content of the bee venom was ably determined. This study provides basic data on cadaverine in bee venom, which will prove useful in further studies on the bioactivity of this component.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"17 1","pages":"144-148"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75342012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.115
Hyeon-Mi Kim, Sung-Ha Kim
Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs’ functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4°C and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.
钙依赖性蛋白激酶(CDPKs)参与调节植物对盐度、低温和脱水等非生物胁迫的反应。虽然CDPKs在水稻中构成了一个由31个基因组成的多基因家族，但目前只发现了一些水稻CDPKs的功能。因此，为了阐明OsCPK11在水稻中的功能，本研究拟重点分析这些非生物胁迫下OsCPK11在野生型和ND0001型OsCPK11突变体植株中的表达模式。在盐胁迫、冷胁迫和干旱胁迫下，幼苗分别处理200 mM NaCl、4°C和20% PEG 6000。RT-PCR和实时荧光定量PCR检测了OsCPK11在野生型和ND0001突变株中的表达模式。RT-PCR结果显示，在野生型和杂合子突变体中检测到OsCPK11转录本，而在纯合子突变体中未检测到。实时荧光定量PCR结果显示，野生型植物OsCPK11的相对表达量在盐胁迫、冷胁迫和干旱胁迫条件下分别在24小时、6小时和24小时达到最高水平。与野生型相比，ND0001纯合子植株OsCPK11的相对表达量显著降低。这些结果表明，oscpk11纯合突变体敲除oscpk11, oscpk11可能通过调控其基因表达参与盐、冷、干旱胁迫信号传导。
{"title":"Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions","authors":"Hyeon-Mi Kim, Sung-Ha Kim","doi":"10.5352/JLS.2021.31.2.115","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.115","url":null,"abstract":"Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs’ functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4°C and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"48 1","pages":"115-125"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87978661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.137
S. Choi, Mina Kim, H. H. L. Lee, J. Hur
Muscle atrophy refers to a decrease in muscle cells due to damage to muscle fibers. It is reported that muscle atrophy is caused by heart disease, diabetes, and other chronic diseases related to aging. The purpose of this study is to reveal the inhibitory effects of seaweed extracts, which are widely consumed in Korea, and alginic acid on muscle cell damage in muscle atrophy and regeneration models. We found that seaweed extracts (U) and alginic acid (A) attenuated TNF-α-induced muscle atrophy in differentiated C2C12 myoblast cells and inhibited muscle atrophy markers such as MuRF1 and MAFbx. In addition, U and A also regulated ubiquitination marker FoxO1 protein. To confirm the muscle regeneration effect in animal tissue, cardiotoxin (CTX) was used for the regeneration model. Six hours after CTX injection, gastrocnemius muscle volume was increased compared to control. Otherwise, the muscle volume of the U and A treatment groups was not changed. U and A also upregulated regeneration markers MyHC and PGC-1α in a CTX mouse model. These results indicate that seaweed extracts and alginic acid, a seaweed component, are applicable to senile sarcopenia by inhibiting muscle loss and promoting muscle regeneration.
{"title":"Undaria pinnatifida Extracts and Alginic Acid Attenuated Muscle Atrophy in TNF-α Induced Myoblast Cells through MAFbx Signaling Cascade","authors":"S. Choi, Mina Kim, H. H. L. Lee, J. Hur","doi":"10.5352/JLS.2021.31.2.137","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.137","url":null,"abstract":"Muscle atrophy refers to a decrease in muscle cells due to damage to muscle fibers. It is reported that muscle atrophy is caused by heart disease, diabetes, and other chronic diseases related to aging. The purpose of this study is to reveal the inhibitory effects of seaweed extracts, which are widely consumed in Korea, and alginic acid on muscle cell damage in muscle atrophy and regeneration models. We found that seaweed extracts (U) and alginic acid (A) attenuated TNF-α-induced muscle atrophy in differentiated C2C12 myoblast cells and inhibited muscle atrophy markers such as MuRF1 and MAFbx. In addition, U and A also regulated ubiquitination marker FoxO1 protein. To confirm the muscle regeneration effect in animal tissue, cardiotoxin (CTX) was used for the regeneration model. Six hours after CTX injection, gastrocnemius muscle volume was increased compared to control. Otherwise, the muscle volume of the U and A treatment groups was not changed. U and A also upregulated regeneration markers MyHC and PGC-1α in a CTX mouse model. These results indicate that seaweed extracts and alginic acid, a seaweed component, are applicable to senile sarcopenia by inhibiting muscle loss and promoting muscle regeneration.","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"14 1","pages":"137-143"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81256040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.199
Soojung Jin, Y. Oh, Y. Son, Soobin Bae, Jung-Ha Park, B. W. Kim, H. Kwon
{"title":"Antioxidant and Anticancer Activities of Euonymus porphyreus Extract in Human Lung Cancer Cells A549","authors":"Soojung Jin, Y. Oh, Y. Son, Soobin Bae, Jung-Ha Park, B. W. Kim, H. Kwon","doi":"10.5352/JLS.2021.31.2.199","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.199","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"6 1","pages":"199-208"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81639479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.175
J. Seo, M. Ryu, Hee-Jong Yang, Su-Ji Jeong, Do-Youn Jeong
{"title":"The Antioxidant and Skin-whitening Effects of Saccharomyces cerevisiae FT4-4 Isolated from Berries Grown in Sunchang","authors":"J. Seo, M. Ryu, Hee-Jong Yang, Su-Ji Jeong, Do-Youn Jeong","doi":"10.5352/JLS.2021.31.2.175","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.175","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"110 1","pages":"175-182"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85223919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fucoidan Stimulates Glucose Uptake via the PI3K/AMPK Pathway and Increases Insulin Sensitivity in 3T3-L1 Adipocytes","authors":"Ji Hee Lee, J. E. Park, J. S. Han","doi":"10.5352/JLS.2021.31.1.1","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.1.1","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"17 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91178170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.1.47
D. Kang, Kyu-Min Kim, Ye-Seong Kim, Yuki Seo, Da-Yeong Song, Dasol Oh, Si-On Choi, J. Hwang, Sam-Woong Kim, K. Bang, S. Gal
{"title":"Inhibition of Tyrosinase by Metabolites Originating from Thrichoderma atroviride","authors":"D. Kang, Kyu-Min Kim, Ye-Seong Kim, Yuki Seo, Da-Yeong Song, Dasol Oh, Si-On Choi, J. Hwang, Sam-Woong Kim, K. Bang, S. Gal","doi":"10.5352/JLS.2021.31.1.47","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.1.47","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"20 1","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76643609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.1.28
D. Ray, Yeongwon Kim, unjeong Choe, H. Kang
{"title":"OmpR Is Essential for Growth and Expression of Virulence-related Genes in the Fish Pathogen Edwardsiella piscicida","authors":"D. Ray, Yeongwon Kim, unjeong Choe, H. Kang","doi":"10.5352/JLS.2021.31.1.28","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.1.28","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"75 1","pages":"28-36"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74500172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.237
Jihee Kim, Young-Hee Kim
{"title":"NLRP3 Inflammasome in Neuroinflammatory Disorders","authors":"Jihee Kim, Young-Hee Kim","doi":"10.5352/JLS.2021.31.2.237","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.237","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"31 1","pages":"237-247"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74022603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.2.219
Il-Suk Kim, W. Bang, K. Bang, Sam-Woong Kim
{"title":"Proteomic Study for Low Molecular Weight Peptides in the Mealworm Tenebrio molitor","authors":"Il-Suk Kim, W. Bang, K. Bang, Sam-Woong Kim","doi":"10.5352/JLS.2021.31.2.219","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.2.219","url":null,"abstract":"","PeriodicalId":16322,"journal":{"name":"Journal of Life Science","volume":"121 2 1","pages":"219-222"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80186422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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