M. Yakubu, Adenike M Oyagoke, Luqman A. Quadri, A. O. Agboola, H. Oloyede
Background: Ethanol extract of Mirabilis jalapa leaf (EEMJL) has been used in the folk medicine of Nigeria as diuretics without any scientific evidence.Aim: Ethanol extract of Mirabilis jalapa leaf at 200, 400 and 600 mg/kg body weight was investigated for diuretic activity in male Wistar rats.Setting: Fresh leaf of M. jalapa was collected from a farmland at the Alanamu area in Ilorin, Kwara State, Nigeria, authenticated and processed for the study.Methods: Thirty male rats (231.50 g ± 13.51 g) were assigned into five groups (A–E) of six rats each. Rats in group A (control) received 1.0 mL of physiological saline (the vehicle). Animals in groups B (positive control), C, D and E received 1.0 mL equivalent to 100 mg/kg body weight of furosemide, 200, 400 and 600 mg/kg body weight of EEMJL, respectively. All administrations were done by oral gavage. The animals were monitored for indicators of diuresis for 5 h using standard methods.Results: Ethanol extract of Mirabilis jalapa leaf dose-dependently increased (p < 0.05) urine volume, urine concentrations of Na+, K+ and Cl− and decreased (p < 0.05) the body weight of the animals. Ethanol extract of Mirabilis jalapa leaf increased the urine pH, saliuretic activity, saliuretic index, Na+ index, K+ index, Cl− index, diuretic action (diuretic index), kaliuretic index, Lipschitz value and percentage saline load excreted, whereas the latency of urination, natriuretic index, carbonic anhydrase inhibitory activity and carbonic anhydrase inhibition index were decreased. The EEMJL treatment-related changes in these parameters were essentially similar to those of the furosemide-treated animals.Conclusion: This study has thus validated diuretic activity of M. jalapa leaf with the 600 mg/kg body weight of EEMJL being the most effective.
{"title":"Diuretic activity of ethanol extract of Mirabilis jalapa (Linn.) leaf in normal male Wistar rats","authors":"M. Yakubu, Adenike M Oyagoke, Luqman A. Quadri, A. O. Agboola, H. Oloyede","doi":"10.4102/JOMPED.V3I1.70","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.70","url":null,"abstract":"Background: Ethanol extract of Mirabilis jalapa leaf (EEMJL) has been used in the folk medicine of Nigeria as diuretics without any scientific evidence.Aim: Ethanol extract of Mirabilis jalapa leaf at 200, 400 and 600 mg/kg body weight was investigated for diuretic activity in male Wistar rats.Setting: Fresh leaf of M. jalapa was collected from a farmland at the Alanamu area in Ilorin, Kwara State, Nigeria, authenticated and processed for the study.Methods: Thirty male rats (231.50 g ± 13.51 g) were assigned into five groups (A–E) of six rats each. Rats in group A (control) received 1.0 mL of physiological saline (the vehicle). Animals in groups B (positive control), C, D and E received 1.0 mL equivalent to 100 mg/kg body weight of furosemide, 200, 400 and 600 mg/kg body weight of EEMJL, respectively. All administrations were done by oral gavage. The animals were monitored for indicators of diuresis for 5 h using standard methods.Results: Ethanol extract of Mirabilis jalapa leaf dose-dependently increased (p < 0.05) urine volume, urine concentrations of Na+, K+ and Cl− and decreased (p < 0.05) the body weight of the animals. Ethanol extract of Mirabilis jalapa leaf increased the urine pH, saliuretic activity, saliuretic index, Na+ index, K+ index, Cl− index, diuretic action (diuretic index), kaliuretic index, Lipschitz value and percentage saline load excreted, whereas the latency of urination, natriuretic index, carbonic anhydrase inhibitory activity and carbonic anhydrase inhibition index were decreased. The EEMJL treatment-related changes in these parameters were essentially similar to those of the furosemide-treated animals.Conclusion: This study has thus validated diuretic activity of M. jalapa leaf with the 600 mg/kg body weight of EEMJL being the most effective.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.70","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44064022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Being natural, herbal products are often perceived as safe but studies have shown that they may not be free of pathogenic microorganisms. The extent of microbial contaminants of traditional oral powdered herbal formulations (TOPHFs) in Ogbomoso, Nigeria, is not known, while the frequency of users of these drugs in the study area has not been reported.Aim: To evaluate the microbial contaminants of TOPHFs marketed in Ogbomoso Nigeria, identify the risk factors associated with their production and handling, and determine the frequency of users of the drugs.Setting: Ogbomoso, Nigeria.Methods: Fifteen manufacturers of TOPHFs listed the health conditions that are indicated for their drugs, while 125 residents provided information on their preference for those drugs. The questionnaires were analysed using descriptive statistics (frequency counts and percentages). Packaging and/or handling and level of personal and environmental hygiene of the manufacturers were examined and analysed for significance of their influence on contamination of drugs using chi-square test. Using pour plate method and following standard procedures, 12 samples of TOPHFs were analysed to quantify their bacterial and fungal contaminants.Results: The TOPHFs were indicated for 17 different types of health conditions with 60% of the sampled population being prolonged and regular users. The personal and environmental hygiene levels of the manufacturers fell short of acceptable standard. Ten out of the 12 samples analysed were heavily contaminated (i.e. above WHO limits) with yeasts/moulds and faecal coliforms such as Enterobacteria, 9 with Streptococcus and 6 with pathogenic bacterial species such as Staphylococcus and Salmonella. There was a significant association of TOPHFs contamination with manufacturers’ level of formal education (p = 0.001), solid waste management (p = 0.015) and methods of updating knowledge and expertise (p = 0.004), thus making these 3 the risk factors associated with contamination of the drugs.Conclusion: Use of TOPHFs is popular among the residents of Ogbomoso, but there is a high rate of contamination of these drugs with some potentially pathogenic organisms because of insufficient quality control in their production and handling.
{"title":"User frequency and microbial contaminants of traditional oral powdered herbal formulations in Ogbomoso, Nigeria","authors":"J. E. Ideh, A. Ogunkunle","doi":"10.4102/JOMPED.V3I1.67","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.67","url":null,"abstract":"Background: Being natural, herbal products are often perceived as safe but studies have shown that they may not be free of pathogenic microorganisms. The extent of microbial contaminants of traditional oral powdered herbal formulations (TOPHFs) in Ogbomoso, Nigeria, is not known, while the frequency of users of these drugs in the study area has not been reported.Aim: To evaluate the microbial contaminants of TOPHFs marketed in Ogbomoso Nigeria, identify the risk factors associated with their production and handling, and determine the frequency of users of the drugs.Setting: Ogbomoso, Nigeria.Methods: Fifteen manufacturers of TOPHFs listed the health conditions that are indicated for their drugs, while 125 residents provided information on their preference for those drugs. The questionnaires were analysed using descriptive statistics (frequency counts and percentages). Packaging and/or handling and level of personal and environmental hygiene of the manufacturers were examined and analysed for significance of their influence on contamination of drugs using chi-square test. Using pour plate method and following standard procedures, 12 samples of TOPHFs were analysed to quantify their bacterial and fungal contaminants.Results: The TOPHFs were indicated for 17 different types of health conditions with 60% of the sampled population being prolonged and regular users. The personal and environmental hygiene levels of the manufacturers fell short of acceptable standard. Ten out of the 12 samples analysed were heavily contaminated (i.e. above WHO limits) with yeasts/moulds and faecal coliforms such as Enterobacteria, 9 with Streptococcus and 6 with pathogenic bacterial species such as Staphylococcus and Salmonella. There was a significant association of TOPHFs contamination with manufacturers’ level of formal education (p = 0.001), solid waste management (p = 0.015) and methods of updating knowledge and expertise (p = 0.004), thus making these 3 the risk factors associated with contamination of the drugs.Conclusion: Use of TOPHFs is popular among the residents of Ogbomoso, but there is a high rate of contamination of these drugs with some potentially pathogenic organisms because of insufficient quality control in their production and handling.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.67","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46514260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. O. Dosumu, Oluwatoyin O. Ajetumobi, O. A. Omole, P. Onocha
Background: Pergularia daemia of the Asteraceae family has wide use in ethno-medicine. It is established to have pharmacologicall activity with potential medicinal significance as an antifungal, antioxidant, antimicrobial, antiinflamatory, analgesic, fertility, hepatoprotective and antic ancer agent . Aim: This study estimated the qualitative and quantitative amount of of phytochemicals in Pergularia daemia plant and the and antioxidant capacity was also determined. Setting: The fresh plants of P. Daemia were collected from Ido-Ekiti, Ekiti State, Nigeria and was identified at Plant Biology Department of the University of Ilorin, Nigeria. The samples were air-dried, pulverized and stored properly for investigation. Method: Both qualitative and quantitative phytochemical analyses of the leaves and stem were determined through n-hexane, ethyl acetate and ethanol extracts of P. daemia . Antioxidant activities of the extracts were evaluated with 2,2-diphenyl-1-picryhydroxyl (DPPH). The antibacterial and antifungal assay of the extracts were determing by dissolving 1 mg of the extract in 5 mL of the extracting solvent to give a 200 µg/mL solution. Results: Flavonoids, steroids, terpenoids and glycosides were present in all the stem and leaf extracts of P. daemia . The total phenolic content of the extracts ranged from 15.898 ± 0.111 mgGA/g to 54.679 ± 0.675 mgGA/g. The leaf has higher concentration of flavonoids than the stem and the ethanol extracts contain highest content of flavonoids. The concentration of the ethanol extract of the leaf was 400.196 mg QE/g while that of ethyl acetate and n-hexane were 388.627 mg QE/g and 338.725 mg QE/g respectively at 10 mg/mL. Ethanol extracts has the highest concentration of phenolic compounds, flavonoids and antioxidant capacity. Conclusion: The results derived from this study validate the ethno-medicinal use of the aerial parts of P. daemia .
{"title":"Phytochemical composition and antioxidant and antimicrobial activities of Pergularia daemia","authors":"O. O. Dosumu, Oluwatoyin O. Ajetumobi, O. A. Omole, P. Onocha","doi":"10.4102/JOMPED.V3I1.26","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.26","url":null,"abstract":"Background: Pergularia daemia of the Asteraceae family has wide use in ethno-medicine. It is established to have pharmacologicall activity with potential medicinal significance as an antifungal, antioxidant, antimicrobial, antiinflamatory, analgesic, fertility, hepatoprotective and antic ancer agent . Aim: This study estimated the qualitative and quantitative amount of of phytochemicals in Pergularia daemia plant and the and antioxidant capacity was also determined. Setting: The fresh plants of P. Daemia were collected from Ido-Ekiti, Ekiti State, Nigeria and was identified at Plant Biology Department of the University of Ilorin, Nigeria. The samples were air-dried, pulverized and stored properly for investigation. Method: Both qualitative and quantitative phytochemical analyses of the leaves and stem were determined through n-hexane, ethyl acetate and ethanol extracts of P. daemia . Antioxidant activities of the extracts were evaluated with 2,2-diphenyl-1-picryhydroxyl (DPPH). The antibacterial and antifungal assay of the extracts were determing by dissolving 1 mg of the extract in 5 mL of the extracting solvent to give a 200 µg/mL solution. Results: Flavonoids, steroids, terpenoids and glycosides were present in all the stem and leaf extracts of P. daemia . The total phenolic content of the extracts ranged from 15.898 ± 0.111 mgGA/g to 54.679 ± 0.675 mgGA/g. The leaf has higher concentration of flavonoids than the stem and the ethanol extracts contain highest content of flavonoids. The concentration of the ethanol extract of the leaf was 400.196 mg QE/g while that of ethyl acetate and n-hexane were 388.627 mg QE/g and 338.725 mg QE/g respectively at 10 mg/mL. Ethanol extracts has the highest concentration of phenolic compounds, flavonoids and antioxidant capacity. Conclusion: The results derived from this study validate the ethno-medicinal use of the aerial parts of P. daemia .","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43899032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The foremost requirements in quality control of a herbal drug are its identity and purity. In addition, information is necessary on whether continual exploitation of medicinal herbs for traditional oral powdered herbal formulations (TOPHFs) in Ogbomoso, Nigeria, is sustainable. Aim: To botanically characterise and ethno-medicinally document the health indications of TOPHFs manufactured in Ogbomoso, as well as to examine the sustainability status of the drugs. Setting: Ogbomoso, Nigeria. Methods: Fifteen manufacturers of TOPHFs provided information on the botanical constituents and recipes of their products, the sources of raw material herbs, and types of health conditions treated with the drugs. Sustainability status of the drugs was quantified as relative percentage of the three choices of sources of raw material herbs available to the manufacturers and in terms of conservation status of the plant species as recorded by the International Union for Conservation of Nature. Results: Fifty-five medicinal plant species from 33 angiosperm families were used by traditional herbal medical practitioners to produce 68 TOPHFs that are indicated for treating 17 different health conditions. The sources of raw material herbs, in relative terms, were purchased from herbal markets (43.8%), collected from the wild (28.1%) and cultivated (28.1%). Most of the herbs can be sustainably harvested and only 3 (i.e. 5.5%) of the 55 plant species (i.e. Lophira alata Banks ex Gaertn., Khaya senegalensis A. Juss. and Garcinia kola Heckel) are under threatened (vulnerable) species. Conclusion: Production of TOPHFs in Ogbomoso is sustainable with minimal injury on the natural flora.
{"title":"Botanical characterisation, drug indications and sustainability status of traditional oral powdered herbal formulations in Ogbomoso, Nigeria","authors":"J. E. Ideh, A. Ogunkunle, M. A. Jimoh","doi":"10.4102/JOMPED.V3I1.64","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.64","url":null,"abstract":"Background: The foremost requirements in quality control of a herbal drug are its identity and purity. In addition, information is necessary on whether continual exploitation of medicinal herbs for traditional oral powdered herbal formulations (TOPHFs) in Ogbomoso, Nigeria, is sustainable. Aim: To botanically characterise and ethno-medicinally document the health indications of TOPHFs manufactured in Ogbomoso, as well as to examine the sustainability status of the drugs. Setting: Ogbomoso, Nigeria. Methods: Fifteen manufacturers of TOPHFs provided information on the botanical constituents and recipes of their products, the sources of raw material herbs, and types of health conditions treated with the drugs. Sustainability status of the drugs was quantified as relative percentage of the three choices of sources of raw material herbs available to the manufacturers and in terms of conservation status of the plant species as recorded by the International Union for Conservation of Nature. Results: Fifty-five medicinal plant species from 33 angiosperm families were used by traditional herbal medical practitioners to produce 68 TOPHFs that are indicated for treating 17 different health conditions. The sources of raw material herbs, in relative terms, were purchased from herbal markets (43.8%), collected from the wild (28.1%) and cultivated (28.1%). Most of the herbs can be sustainably harvested and only 3 (i.e. 5.5%) of the 55 plant species (i.e. Lophira alata Banks ex Gaertn., Khaya senegalensis A. Juss. and Garcinia kola Heckel) are under threatened (vulnerable) species. Conclusion: Production of TOPHFs in Ogbomoso is sustainable with minimal injury on the natural flora.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.64","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47513921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Lawal, Hi Ajayi, Paul O. Dairo, Paul Oluwatimilehin Ogunbamowo
Background: Essential oils have been used for medical purposes because of their medicinal properties and are sourced from natural products. Aim: The comparative study of essential oils from Clausena anisata leaves obtained through two methods of extraction was undertaken. Setting: C. anisata leaves were collected from Akinsola village in Ido Local Government Area of Oyo State, Nigeria. Methods: Essential oil was extracted using the methods of solvent-free microwave extraction (SFME) and hydrodistillation (HD) and then analysed using gas chromatography coupled with mass spectrometer (GC-MS). Results: The result shows that the essential oils obtained from the leaves of C. anisata through SFME and HD are pale yellow and colourless, respectively. A higher oil yield of 5.2 mL/100 g was obtained through SFME compared to 3.7 mL/100 g obtained through HD. There was an abundance of monoterpenes in the oil obtained using HD, while sesquiterpenes were the dominant compounds in the oil extracted using SFME. Other compounds present were α-cubebene, α-copaene and α-pinene with 1091.63, 1091.64 and 979.35, respectively. Conclusion: The compounds obtained which are very important in the cosmeceutical and pharmaceutical industries, is dependent on the method of essential oil extraction.
{"title":"Comparative studies of essential oils of Clausena anisata (Hook) using solvent-free microwave extraction and hydrodistillation methods","authors":"I. Lawal, Hi Ajayi, Paul O. Dairo, Paul Oluwatimilehin Ogunbamowo","doi":"10.4102/JOMPED.V3I1.59","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.59","url":null,"abstract":"Background: Essential oils have been used for medical purposes because of their medicinal properties and are sourced from natural products. Aim: The comparative study of essential oils from Clausena anisata leaves obtained through two methods of extraction was undertaken. Setting: C. anisata leaves were collected from Akinsola village in Ido Local Government Area of Oyo State, Nigeria. Methods: Essential oil was extracted using the methods of solvent-free microwave extraction (SFME) and hydrodistillation (HD) and then analysed using gas chromatography coupled with mass spectrometer (GC-MS). Results: The result shows that the essential oils obtained from the leaves of C. anisata through SFME and HD are pale yellow and colourless, respectively. A higher oil yield of 5.2 mL/100 g was obtained through SFME compared to 3.7 mL/100 g obtained through HD. There was an abundance of monoterpenes in the oil obtained using HD, while sesquiterpenes were the dominant compounds in the oil extracted using SFME. Other compounds present were α-cubebene, α-copaene and α-pinene with 1091.63, 1091.64 and 979.35, respectively. Conclusion: The compounds obtained which are very important in the cosmeceutical and pharmaceutical industries, is dependent on the method of essential oil extraction.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.59","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47580981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: A substantial number of drugs are being developed from plants for the treatment of various diseases. Lannea egregia (LE) is a woody perennial plant used traditionally in the management of skin disorders and wounds.Aim: To investigate the biological activities of different solvent extracts of LE bark.Settings: Fresh stem bark of Lannea egregia was collected from Itabo Lanlate, Oyo State, Nigeria. The authentication was done in Forest Herbarium, Ibadan (FHI), Nigeria. The biological activities of the air-dried sample were carried out in the Department of Pharmacognosy, University of Ibadan, Nigeria.Materials and methods: Powdered LE bark sample was extracted by gradient extraction. Phytochemical screening was performed on the extracts using standard procedure. In vitro antimicrobial study was performed on 14 strains of bacteria and 5 fungal strains at a concentration range of 25, 50, 75 and 100 mg/mL of the extract using ciprofloxacin and itraconazole as standard. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and total phenolic content (TPC) of two active extracts were determined spectrophotometrically.Results: The phytochemical screening of LE revealed the presence of tannins, terpenoids, flavonoids, anthraquinones, saponins and alkaloids. The dichloromethane (DCM) extract exhibited the highest activity against all the bacterial strains as well as four of the fungal strains. The zones of inhibition (ZI) of bacteria ranged from 9.0 ± 2.0 to 24.6 ± 2.4 mm, MIC of 0.0008 to 12.5 mg/mL and MBC of 25 to 75 mg/mL, while ZI of the fungal strains ranged from 10.3 ± 4.6 to 18.0 ± 5.3 mm, MIC 0.391 – 0.781 and MFC of 50 mg/mL in all the strains. The TPC values of DCM and ethyl acetate (EtOAc) extracts were 1582.47 ± 6.69 and 1579.89 ± 12.77 µg GAE/mg and inhibitory antioxidant activity of 2.54 ± 0.58 and 2.44 ± 0.54, respectively.Conclusion: These findings provide scientific evidence to support the ethnomedicinal use of Lannea egregia bark for treating skin disorders and wounds.
{"title":"Phytochemical screening, antimicrobial and antioxidant studies of Lannea egregia Engl. and K. Krause (Anacardiaceae) stem bark","authors":"B. Rafiu, Adeola M. Sonibare, E. O. Adesanya","doi":"10.4102/JOMPED.V3I1.62","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.62","url":null,"abstract":"Background: A substantial number of drugs are being developed from plants for the treatment of various diseases. Lannea egregia (LE) is a woody perennial plant used traditionally in the management of skin disorders and wounds.Aim: To investigate the biological activities of different solvent extracts of LE bark.Settings: Fresh stem bark of Lannea egregia was collected from Itabo Lanlate, Oyo State, Nigeria. The authentication was done in Forest Herbarium, Ibadan (FHI), Nigeria. The biological activities of the air-dried sample were carried out in the Department of Pharmacognosy, University of Ibadan, Nigeria.Materials and methods: Powdered LE bark sample was extracted by gradient extraction. Phytochemical screening was performed on the extracts using standard procedure. In vitro antimicrobial study was performed on 14 strains of bacteria and 5 fungal strains at a concentration range of 25, 50, 75 and 100 mg/mL of the extract using ciprofloxacin and itraconazole as standard. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and total phenolic content (TPC) of two active extracts were determined spectrophotometrically.Results: The phytochemical screening of LE revealed the presence of tannins, terpenoids, flavonoids, anthraquinones, saponins and alkaloids. The dichloromethane (DCM) extract exhibited the highest activity against all the bacterial strains as well as four of the fungal strains. The zones of inhibition (ZI) of bacteria ranged from 9.0 ± 2.0 to 24.6 ± 2.4 mm, MIC of 0.0008 to 12.5 mg/mL and MBC of 25 to 75 mg/mL, while ZI of the fungal strains ranged from 10.3 ± 4.6 to 18.0 ± 5.3 mm, MIC 0.391 – 0.781 and MFC of 50 mg/mL in all the strains. The TPC values of DCM and ethyl acetate (EtOAc) extracts were 1582.47 ± 6.69 and 1579.89 ± 12.77 µg GAE/mg and inhibitory antioxidant activity of 2.54 ± 0.58 and 2.44 ± 0.54, respectively.Conclusion: These findings provide scientific evidence to support the ethnomedicinal use of Lannea egregia bark for treating skin disorders and wounds.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.62","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47270126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Medicinal plants are a potent source of therapeutic molecules that heal various diseases in the world.Aim: The aim of this study was to evaluate heavy metal concentrations in the leaves of some selected medicinal plants in selected locations.Setting: The leaves of Azadirachta indica, Magnifera indica and Newbouldia laevis were collected from the botanical garden, roadside and residential area in the University of Ibadan and were authenticated at the Forest Herbarium Ibadan. The samples were thoroughly washed with deionised water and air-dried at room temperature for about three weeks, then ground into powder with a mechanical grinder. The samples were subsequently stored in air-tight bottles for further work.Methods: The milled samples were subjected to wet digestion, and then lead (Pb), chromium (Cr), cadmium (Cd) and nickel (Ni) were analysed using standard methods.Results: The results obtained show that the highest concentrations of Pb, Cr and Cd in the medicinal plants studied were found in N. laevis (23.93 ppm; road side), N. laevis (4.79 ppm; road side) and M. indica (0.36 ppm; road side), respectively, while the lowest concentrations of Pb, Cr and Cd were found in A. indica (7.10 ppm; botanical garden), N. laevis (0.73 ppm; residential) and N. laevis (0.05 ppm; residential), respectively. Ni was totally undetected in all three medicinal plants studied in all the locations.Conclusion: The concentrations of heavy metals at the roadside and residential area were higher than permissible limits set for medicinal plants, but the botanical garden was the lowest and safest in terms of heavy metals accumulation in the medicinal plants studied in the three locations.
{"title":"Evaluation of heavy metals in some selected medicinal plants growing within the University of Ibadan Campus","authors":"S. Rufai, M. B. Olaniyi, I. Lawal","doi":"10.4102/JOMPED.V3I1.63","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.63","url":null,"abstract":"Background: Medicinal plants are a potent source of therapeutic molecules that heal various diseases in the world.Aim: The aim of this study was to evaluate heavy metal concentrations in the leaves of some selected medicinal plants in selected locations.Setting: The leaves of Azadirachta indica, Magnifera indica and Newbouldia laevis were collected from the botanical garden, roadside and residential area in the University of Ibadan and were authenticated at the Forest Herbarium Ibadan. The samples were thoroughly washed with deionised water and air-dried at room temperature for about three weeks, then ground into powder with a mechanical grinder. The samples were subsequently stored in air-tight bottles for further work.Methods: The milled samples were subjected to wet digestion, and then lead (Pb), chromium (Cr), cadmium (Cd) and nickel (Ni) were analysed using standard methods.Results: The results obtained show that the highest concentrations of Pb, Cr and Cd in the medicinal plants studied were found in N. laevis (23.93 ppm; road side), N. laevis (4.79 ppm; road side) and M. indica (0.36 ppm; road side), respectively, while the lowest concentrations of Pb, Cr and Cd were found in A. indica (7.10 ppm; botanical garden), N. laevis (0.73 ppm; residential) and N. laevis (0.05 ppm; residential), respectively. Ni was totally undetected in all three medicinal plants studied in all the locations.Conclusion: The concentrations of heavy metals at the roadside and residential area were higher than permissible limits set for medicinal plants, but the botanical garden was the lowest and safest in terms of heavy metals accumulation in the medicinal plants studied in the three locations.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.63","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46966049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Adeoye, T. Ajibade, A. Oyagbemi, T. Omobowale, M. Yakubu, A. Adedapo, A. Ayodele, A. Adedapo
Background: Myocardial infarction has been regarded as one of the fastest killer diseases of modern-day man. Aim: The protective effect of Andrographis paniculata on isoproterenol (ISO)-induced myocardial infarction in rats was investigated. Setting: The study was carried out in a laboratory setting. Methods: Animals were randomly divided into six groups of seven animals per group, and the treatment was as follows: normal control received normal saline for 9 days, isoproterenol group; three extract-treated groups in pre-treatment phase and an extract-treated group in post-treatment phase. The doses were given at 100, 200 and 400 mg/kg body weight for pre-treatment phase respectively while 200 mg/kg dose was given to the post-treatment phase group. Blood and heart tissues were collected for biochemical assays, haematological and histological analyses. Results: Myocardial infarction was recorded in ISO group but was corrected by the extracts in both pre-treatment and post-treatment phases. The ISO group experienced a significant decrease in antioxidant parameters, whereas the extract at all doses caused a significant increase in the activities of in these parameters. The extract caused a significant decrease in malondialdehyde content and hydrogen peroxide generation, whereas reverse was the case for the ISO group. Although no significant histopathological changes were recorded for the extract, the ISO group showed marked histopathological changes. ISO caused higher expressions of cardiac C-reactive protein (CRP) and CTnI and decreased the expressions of IL-10β; but this was the opposite for the extract. Conclusion: The ethanol leaf extract of A. paniculata significantly exhibits cardioprotective effects.
{"title":"Cardioprotective effects and antioxidant status of Andrographis paniculata in isoproterenol-induced myocardial infarction in rats","authors":"B. Adeoye, T. Ajibade, A. Oyagbemi, T. Omobowale, M. Yakubu, A. Adedapo, A. Ayodele, A. Adedapo","doi":"10.4102/JOMPED.V3I1.49","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.49","url":null,"abstract":"Background: Myocardial infarction has been regarded as one of the fastest killer diseases of modern-day man. Aim: The protective effect of Andrographis paniculata on isoproterenol (ISO)-induced myocardial infarction in rats was investigated. Setting: The study was carried out in a laboratory setting. Methods: Animals were randomly divided into six groups of seven animals per group, and the treatment was as follows: normal control received normal saline for 9 days, isoproterenol group; three extract-treated groups in pre-treatment phase and an extract-treated group in post-treatment phase. The doses were given at 100, 200 and 400 mg/kg body weight for pre-treatment phase respectively while 200 mg/kg dose was given to the post-treatment phase group. Blood and heart tissues were collected for biochemical assays, haematological and histological analyses. Results: Myocardial infarction was recorded in ISO group but was corrected by the extracts in both pre-treatment and post-treatment phases. The ISO group experienced a significant decrease in antioxidant parameters, whereas the extract at all doses caused a significant increase in the activities of in these parameters. The extract caused a significant decrease in malondialdehyde content and hydrogen peroxide generation, whereas reverse was the case for the ISO group. Although no significant histopathological changes were recorded for the extract, the ISO group showed marked histopathological changes. ISO caused higher expressions of cardiac C-reactive protein (CRP) and CTnI and decreased the expressions of IL-10β; but this was the opposite for the extract. Conclusion: The ethanol leaf extract of A. paniculata significantly exhibits cardioprotective effects.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44530059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. K. Asekunowo, A. Ashafa, O. Okoh, O. Asekun, O. Familoni
Background: Acalypha godseffiana is an important plant used as an ornamental and herbs; its leaves are used in the management of diseases like diabetes in Eastern Nigeria. Aim: The study aims at linking the hypoglycemic activity extracts of leaves of A. godseffiana to their polyphenolic contents. Setting: Fresh leaves of A. godseffiana were obtainedfrom Imo State, Nigeria, identified in University of Lagos Herbarium, air-dried, pulverized and kept for investigations. Method: The phytochemical compositions and antioxidant potentials of acetone, aqueous, ethanol and methanol extracts of A. godseffiana were determined using adopted methods. An in-vitro approach was used to evaluate the hypoglycemic potentials of the extracts on α-amylase and α-glucosidase enzymes. The mechanism of inhibitions was studied using the Lineweaver-Burk plot. Results: Antioxidant results revealed that total antioxidant capacity of the acetone extract (IC50: 0.34 mg/mL) showed better activity compared to the standards (silymarin 0.52 mg/mL; gallic acid 0.51 mg/mL). Thehypoglycemic findings confirmed that acetone extract demonstrated strong and mild inhibitory potential against α-amylase and α-glucosidase respectively, showing concentration dependent with IC 50 values of 2.33 mg/mL and 0.13 mg/mL. The observed hypoglycemic and antioxidant potentials of acetone extract of A. godseffiana correlate to its high polyphenolic contents which include phenols (133.20 mg gallic acid g -1 ), flavonoid (350.60 mg quercetin g -1 ) and tannins (264.67 mg catechin g -1 ). The mechanisms of action exhibited by the acetone extractwere uncompetitive and mixed non-competitive which can be attributed to its inhibitory properties on α-glucosidase and α-amylase respectively. Conclusion: The results obtained from this study validate the acetone leaves extract of A. godseffiana as potential agent in management of sugar related disorder.
{"title":"Polyphenolic constituents, antioxidant and hypoglycaemic potential of leaf extracts of Acalypha godseffiana from Eastern Nigeria: In vitro study","authors":"A. K. Asekunowo, A. Ashafa, O. Okoh, O. Asekun, O. Familoni","doi":"10.4102/JOMPED.V3I1.36","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.36","url":null,"abstract":"Background: Acalypha godseffiana is an important plant used as an ornamental and herbs; its leaves are used in the management of diseases like diabetes in Eastern Nigeria. Aim: The study aims at linking the hypoglycemic activity extracts of leaves of A. godseffiana to their polyphenolic contents. Setting: Fresh leaves of A. godseffiana were obtainedfrom Imo State, Nigeria, identified in University of Lagos Herbarium, air-dried, pulverized and kept for investigations. Method: The phytochemical compositions and antioxidant potentials of acetone, aqueous, ethanol and methanol extracts of A. godseffiana were determined using adopted methods. An in-vitro approach was used to evaluate the hypoglycemic potentials of the extracts on α-amylase and α-glucosidase enzymes. The mechanism of inhibitions was studied using the Lineweaver-Burk plot. Results: Antioxidant results revealed that total antioxidant capacity of the acetone extract (IC50: 0.34 mg/mL) showed better activity compared to the standards (silymarin 0.52 mg/mL; gallic acid 0.51 mg/mL). Thehypoglycemic findings confirmed that acetone extract demonstrated strong and mild inhibitory potential against α-amylase and α-glucosidase respectively, showing concentration dependent with IC 50 values of 2.33 mg/mL and 0.13 mg/mL. The observed hypoglycemic and antioxidant potentials of acetone extract of A. godseffiana correlate to its high polyphenolic contents which include phenols (133.20 mg gallic acid g -1 ), flavonoid (350.60 mg quercetin g -1 ) and tannins (264.67 mg catechin g -1 ). The mechanisms of action exhibited by the acetone extractwere uncompetitive and mixed non-competitive which can be attributed to its inhibitory properties on α-glucosidase and α-amylase respectively. Conclusion: The results obtained from this study validate the acetone leaves extract of A. godseffiana as potential agent in management of sugar related disorder.","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4102/JOMPED.V3I1.36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46047607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bananas in pyjamas","authors":"Jan Piet Salie","doi":"10.4102/JOMPED.V3I1.74","DOIUrl":"https://doi.org/10.4102/JOMPED.V3I1.74","url":null,"abstract":"","PeriodicalId":16345,"journal":{"name":"Journal of Medicinal Plants for Economic Development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45062804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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